Nost cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nost
Entrez Gene ID	8673992
Full Name	Nostrin
Synonyms	CG15364,CG33224,CG4040,CG42388,Dmel\CG42388,Dmel_CG15364,Dmel_CG33224,Dmel_CG4040,NOSTRIN,nost
General protein information	Preferred Names Nostrin Names CG42388 gene product from transcript CG42388-RH CG42388-PC CG42388-PD CG42388-PE CG42388-PF CG42388-PG CG42388-PH Nost-PC Nost-PD Nost-PE Nost-PF Nost-PG Nost-PH nostrin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8C4-8C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169240.2	NP_001162711.1	nostrin, isoform E
NM_001169241.1	NP_001162712.1	nostrin, isoform F
NM_001201638.2	NP_001188567.1	nostrin, isoform G
NM_001298100.1	NP_001285029.1	nostrin, isoform H
NM_001201637.1	NP_001188566.1	nostrin, isoform C
NM_132287.3	NP_572515.2	nostrin, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	NOSTRIN	NP_001034813.2
Mus musculus (house mouse)	Nostrin	NP_853525.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010676	XP_311392.4
Xenopus tropicalis (tropical clawed frog)	nostrin	XP_002936800.2
Danio rerio (zebrafish)	nostrin	NP_001107076.1
Pan troglodytes (chimpanzee)	NOSTRIN	XP_001155546.1
	NOSTRIN	XP_001103102.1
Bos taurus (cattle)	NOSTRIN	NP_001039722.1
Canis lupus familiaris (dog)	NOSTRIN	XP_535945.3
Rattus norvegicus (Norway rat)	Nostrin	NP_001019431.1
Drosophila melanogaster (fruit fly)	CG42388	NP_001162711.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cip4 initially promotes membrane invagination and early-actin-based endosomal motility, and Nostrin makes contacts with microtubules through the kinesin Khc-73 for trafficking of recycling endosomes.
Title: Cooperative functions of the two F-BAR proteins Cip4 and Nostrin in the regulation of E-cadherin in epithelial morphogenesis.

The following Nost gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nost cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02657	NM_001201637.1 Latest version!	Drosophila melanogaster nostrin, transcript variant C (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02658	NM_001169241.1 Latest version!	Drosophila melanogaster nostrin, transcript variant F (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03106	NM_132287.3 Latest version!	Drosophila melanogaster nostrin, transcript variant D (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02659	NM_001201638.2 Latest version!	Drosophila melanogaster nostrin, transcript variant G (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa02659	NM_001169240.2 Latest version!	Drosophila melanogaster nostrin, transcript variant E (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa22440	NM_001298100.1 Latest version!	Drosophila melanogaster nostrin, transcript variant H (Nost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nost ( NM_001201637.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188566.1 Drosophila melanogaster nostrin, transcript variant C (Nost), mRNA.

Price & Availability↑

CloneID	OFa02657
Clone ID Related Accession (Same CDS sequence)	NM_001201637.1
Accession Version	NM_001201637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACAAT TCCGCGATAA TTCATGGCTC GCCAATGCGC GATCATACGG CGTTTTGAAG 
AGTTTGAGAA GCTCGGCACG TTATGCCAAG GCCAAATCCA AGTCGGCCAT TAATCTCAAT 
GCCCATCTGA AGAAGCAGCA GAAGCTGGAG GAAGGACATC CCGAAAAGAA GAGGCAGGAT 
AAGAAAATAC TCAGACAGAG TGTCAGTGAG GATCAGCCGA TCTATGCGAA TGTGGACGAG 
GTTATACCGA TCCATTTAGA ACCGGAAACC GGAACCGAAG TCGATGCTCT AATGGGTAAT 
GCACAGGGAC AGAACGGCTT CGAGGAACTC CGGCGATATG TGAAGCAGGG CGGTGATTTT 
AGCAAGGAGC TCATCTTTGT CCTGCAGGAG AGAGCCGATT CGGAGCTGAT CTACTCCAAG 
TCGCTATCGA AACTGGCCAA CAAGCTGAAC AAAGCGGGCA GGGAAATTCC CGGGAGCGTG 
GCCGACGCCT GGCGTGGAGT GGCTACGGAG ATGGAGAGCC GCAGCGACAT CCATCGCCAG 
TTGGCAGCCT CGCTAACGGA TGAGCTGGTC AAGCCGCTCA AGATCGTCGT CGAAGGACAT 
CACAAGGCGC GCAAGGCGGT GGAAAGCAAC GTGGACAAGG CGGCGCGAGT ACTGGGCGAA 
TGGCGAGCCA GCGAGGCGAA AGCCAAGAAG GCCTCCCATA CGGCGGCGCG CGAGAACGAG 
AAGCTGCAGG ACGCAATGCT GGACGTGCGC ATCCAGAAGT CACCATCGAT TGCCCTGCTC 
CACCAGGGAC CCAACAAGCA GGCCGCAGAA AAGGAGCTCA AGTCAGCGGA GAAGGATTGC 
GTCAAGCTGG ATAACAAGCG CAAGAAGGCC GAGGAGGCGG TGAAGCGTGC GGATGTGGAA 
TACTACACCC TGTGCGTGCG TTCGGAGCGA GCACGCGTAG ACTGGGAGAT GGCCGTGCTC 
CGGGGAAGTG CCCAACTGCA GAGCAGCGAA CAGCAGCGGC TGGGCAATAT GCACAACTTT 
GCCCAGCAGT ATGCACGCCT CATCTCGGAC ATGAATCCGA TACTCGGTGG CCTGAGCACA 
CGCCTGCAGC CGCAACTCGA TGCTTGCAAT GTGGCTAAGG ATATGCAAGT GGTGCGCCAC 
ATCCGCCGCA ATTCGGAGGG ACCCAGTGAG CAACTCCTAC CGGACTTTTA CTGCGAACAC 
ACCACGCTGG CCATGAATCG GGAGCGTCGC AAGCACGCTC TGATCAAGCT GCTGCAGCTG 
GTCAAGACGG ATCTGGAGCG GGAGCGACGA TCCCGCGACG GATTGAGGGG ACTGTCGCAA 
TCGCTTAACC ATCAGGAGCA TCAGAACATC ACCGATAAGC TGTACCACAT TCGTTCCATG 
CTGACCTATT TGGAGGGCGC CCGTCTCAAG CTGCACTCTG CCCTCTTGGA GCTGGACCAC 
AAGCCCAGAG CCACTCACCC ACTGGCCCAG CACATTCATA TCACCCGTGA CCGCACTGGT 
TTGCAGCAGA GCATCCTGAA GGTGCCCAAC TGGCTGAAGA ACAACGACAA GTCGCAGACG 
CAGCAATCGA CCAGTATGCT GGCCCACGAC ATCACCGACA TAACCACTAA CCACGAGGAT 
GAGCTTCCCG ACGAGAACTG CTCCCACCTG CACAGCAGTA GCCATAGCAA CAATAATAGC 
AACGGCTCCT GCACAGATGT CAGTTCGGTG GTGAAGCCTT TTAACCGAAG CAAAAGCAAC 
ATCGAGACCA ATTTCACCAG TCAACCAAAG ATAATCTCGA CGACAAATGC CGCGATTGCC 
GTGTCCGTTA AGTCCAAAAC CCTGGCCAAT CTGCAGGATG GCCATCACAA TAACCAACAG 
CACCATCATC ATCATCATAA TCATCACAGC GATCGCGGCC AGGCGGATGG TGGATCCAAT 
CAGCAGGACT CTGATTTCGA TGAGTTCAGC TCGCAGGACG AGGACGACGA GCCGCAGCAG 
CCGCAGCAAC AACCGCAGCC GTTGCAATCG CAGCAGCAGC AACAGCAGCC GTTGCTGCAA 
AATCCAACCA TCAATGGCCA AGTGCAGACG CAGCATTTCT ATCAGAATGC GCAGGAACTG 
CAGAAGGGCC AAAAGAACGG ATCCGTTCCG ATACTGGGAC GCTGCAAGGC ACTGTATAGC 
TACACCCCGA AGCTCTACGA CGAGCTGGAA CTGAGTCCCG GCGACATCAT CGAGGTGCAT 
GCCAAACAGG ACGACGGCTG GTGGCTGGGC GCCCTGCGCA ATCACATCGG CATCTTTCCG 
GCCACCTATG TGGAGGAGTG CGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188566.1
CDS	666..2972
Translation	MSQFRDNSWLANARSYGVLKSLRSSARYAKAKSKSAINLNAHLKKQQKLEEGHPEKKRQDKKILRQSVSEDQPIYANVDEVIPIHLEPETGTEVDALMGNAQGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_001201637.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant C (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201637.1

ORF Insert Sequence:

ATGTCACAAT TCCGCGATAA TTCATGGCTC GCCAATGCGC GATCATACGG CGTTTTGAAG 
AGTTTGAGAA GCTCGGCACG TTATGCCAAG GCCAAATCCA AGTCGGCCAT TAATCTCAAT 
GCCCATCTGA AGAAGCAGCA GAAGCTGGAG GAAGGACATC CCGAAAAGAA GAGGCAGGAT 
AAGAAAATAC TCAGACAGAG TGTCAGTGAG GATCAGCCGA TCTATGCGAA TGTGGACGAG 
GTTATACCGA TCCATTTAGA ACCGGAAACC GGAACCGAAG TCGATGCTCT AATGGGTAAT 
GCACAGGGAC AGAACGGCTT CGAGGAACTC CGGCGATATG TGAAGCAGGG CGGTGATTTT 
AGCAAGGAGC TCATCTTTGT CCTGCAGGAG AGAGCCGATT CGGAGCTGAT CTACTCCAAG 
TCGCTATCGA AACTGGCCAA CAAGCTGAAC AAAGCGGGCA GGGAAATTCC CGGGAGCGTG 
GCCGACGCCT GGCGTGGAGT GGCTACGGAG ATGGAGAGCC GCAGCGACAT CCATCGCCAG 
TTGGCAGCCT CGCTAACGGA TGAGCTGGTC AAGCCGCTCA AGATCGTCGT CGAAGGACAT 
CACAAGGCGC GCAAGGCGGT GGAAAGCAAC GTGGACAAGG CGGCGCGAGT ACTGGGCGAA 
TGGCGAGCCA GCGAGGCGAA AGCCAAGAAG GCCTCCCATA CGGCGGCGCG CGAGAACGAG 
AAGCTGCAGG ACGCAATGCT GGACGTGCGC ATCCAGAAGT CACCATCGAT TGCCCTGCTC 
CACCAGGGAC CCAACAAGCA GGCCGCAGAA AAGGAGCTCA AGTCAGCGGA GAAGGATTGC 
GTCAAGCTGG ATAACAAGCG CAAGAAGGCC GAGGAGGCGG TGAAGCGTGC GGATGTGGAA 
TACTACACCC TGTGCGTGCG TTCGGAGCGA GCACGCGTAG ACTGGGAGAT GGCCGTGCTC 
CGGGGAAGTG CCCAACTGCA GAGCAGCGAA CAGCAGCGGC TGGGCAATAT GCACAACTTT 
GCCCAGCAGT ATGCACGCCT CATCTCGGAC ATGAATCCGA TACTCGGTGG CCTGAGCACA 
CGCCTGCAGC CGCAACTCGA TGCTTGCAAT GTGGCTAAGG ATATGCAAGT GGTGCGCCAC 
ATCCGCCGCA ATTCGGAGGG ACCCAGTGAG CAACTCCTAC CGGACTTTTA CTGCGAACAC 
ACCACGCTGG CCATGAATCG GGAGCGTCGC AAGCACGCTC TGATCAAGCT GCTGCAGCTG 
GTCAAGACGG ATCTGGAGCG GGAGCGACGA TCCCGCGACG GATTGAGGGG ACTGTCGCAA 
TCGCTTAACC ATCAGGAGCA TCAGAACATC ACCGATAAGC TGTACCACAT TCGTTCCATG 
CTGACCTATT TGGAGGGCGC CCGTCTCAAG CTGCACTCTG CCCTCTTGGA GCTGGACCAC 
AAGCCCAGAG CCACTCACCC ACTGGCCCAG CACATTCATA TCACCCGTGA CCGCACTGGT 
TTGCAGCAGA GCATCCTGAA GGTGCCCAAC TGGCTGAAGA ACAACGACAA GTCGCAGACG 
CAGCAATCGA CCAGTATGCT GGCCCACGAC ATCACCGACA TAACCACTAA CCACGAGGAT 
GAGCTTCCCG ACGAGAACTG CTCCCACCTG CACAGCAGTA GCCATAGCAA CAATAATAGC 
AACGGCTCCT GCACAGATGT CAGTTCGGTG GTGAAGCCTT TTAACCGAAG CAAAAGCAAC 
ATCGAGACCA ATTTCACCAG TCAACCAAAG ATAATCTCGA CGACAAATGC CGCGATTGCC 
GTGTCCGTTA AGTCCAAAAC CCTGGCCAAT CTGCAGGATG GCCATCACAA TAACCAACAG 
CACCATCATC ATCATCATAA TCATCACAGC GATCGCGGCC AGGCGGATGG TGGATCCAAT 
CAGCAGGACT CTGATTTCGA TGAGTTCAGC TCGCAGGACG AGGACGACGA GCCGCAGCAG 
CCGCAGCAAC AACCGCAGCC GTTGCAATCG CAGCAGCAGC AACAGCAGCC GTTGCTGCAA 
AATCCAACCA TCAATGGCCA AGTGCAGACG CAGCATTTCT ATCAGAATGC GCAGGAACTG 
CAGAAGGGCC AAAAGAACGG ATCCGTTCCG ATACTGGGAC GCTGCAAGGC ACTGTATAGC 
TACACCCCGA AGCTCTACGA CGAGCTGGAA CTGAGTCCCG GCGACATCAT CGAGGTGCAT 
GCCAAACAGG ACGACGGCTG GTGGCTGGGC GCCCTGCGCA ATCACATCGG CATCTTTCCG 
GCCACCTATG TGGAGGAGTG CGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nost ( NM_001169241.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162712.1 Drosophila melanogaster nostrin, transcript variant F (Nost), mRNA.

Price & Availability↑

CloneID	OFa02658
Clone ID Related Accession (Same CDS sequence)	NM_001169241.1
Accession Version	NM_001169241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAATG CACAGGGACA GAACGGCTTC GAGGAACTCC GGCGATATGT GAAGCAGGGC 
GGTGATTTTA GCAAGGAGCT CATCTTTGTC CTGCAGGAGA GAGCCGATTC GGAGCTGATC 
TACTCCAAGT CGCTATCGAA ACTGGCCAAC AAGCTGAACA AAGCGGGCAG GGAAATTCCC 
GGGAGCGTGG CCGACGCCTG GCGTGGAGTG GCTACGGAGA TGGAGAGCCG CAGCGACATC 
CATCGCCAGT TGGCAGCCTC GCTAACGGAT GAGCTGGTCA AGCCGCTCAA GATCGTCGTC 
GAAGGACATC ACAAGGCGCG CAAGGCGGTG GAAAGCAACG TGGACAAGGC GGCGCGAGTA 
CTGGGCGAAT GGCGAGCCAG CGAGGCGAAA GCCAAGAAGG CCTCCCATAC GGCGGCGCGC 
GAGAACGAGA AGCTGCAGGA CGCAATGCTG GACGTGCGCA TCCAGAAGTC ACCATCGATT 
GCCCTGCTCC ACCAGGGACC CAACAAGCAG GCCGCAGAAA AGGAGCTCAA GTCAGCGGAG 
AAGGATTGCG TCAAGCTGGA TAACAAGCGC AAGAAGGCCG AGGAGGCGGT GAAGCGTGCG 
GATGTGGAAT ACTACACCCT GTGCGTGCGT TCGGAGCGAG CACGCGTAGA CTGGGAGATG 
GCCGTGCTCC GGGGAAGTGC CCAACTGCAG AGCAGCGAAC AGCAGCGGCT GGGCAATATG 
CACAACTTTG CCCAGCAGTA TGCACGCCTC ATCTCGGACA TGAATCCGAT ACTCGGTGGC 
CTGAGCACAC GCCTGCAGCC GCAACTCGAT GCTTGCAATG TGGCTAAGGA TATGCAAGTG 
GTGCGCCACA TCCGCCGCAA TTCGGAGGGA CCCAGTGAGC AACTCCTACC GGACTTTTAC 
TGCGAACACA CCACGCTGGC CATGAATCGG GAGCGTCGCA AGCACGCTCT GATCAAGCTG 
CTGCAGCTGG TCAAGACGGA TCTGGAGCGG GAGCGACGAT CCCGCGACGG ATTGAGGGGA 
CTGTCGCAAT CGCTTAACCA TCAGGAGCAT CAGAACATCA CCGATAAGCT GTACCACATT 
CGTTCCATGC TGACCTATTT GGAGGGCGCC CGTCTCAAGC TGCACTCTGC CCTCTTGGAG 
CTGGACCACA AGCCCAGAGC CACTCACCCA CTGGCCCAGC ACATTCATAT CACCCGTGAC 
CGCACTGGTT TGCAGCAGAG CATCCTGAAG GTGCCCAACT GGCTGAAGAA CAACGACAAG 
TCGCAGACGC AGCAATCGAC CAGTATGCTG GCCCACGACA TCACCGACAT AACCACTAAC 
CACGAGGATG AGCTTCCCGA CGAGAACTGC TCCCACCTGC ACAGCAGTAG CCATAGCAAC 
AATAATAGCA ACGGCTCCTG CACAGATGTC AGTTCGGTGG TGAAGCCTTT TAACCGAAGC 
AAAAGCAACA TCGAGACCAA TTTCACCAGT CAACCAAAGA TAATCTCGAC GACAAATGCC 
GCGATTGCCG TGTCCGTTAA GTCCAAAACC CTGGCCAATC TGCAGGATGG CCATCACAAT 
AACCAACAGC ACCATCATCA TCATCATAAT CATCACAGCG ATCGCGGCCA GGCGGATGGT 
GGATCCAATC AGCAGGACTC TGATTTCGAT GAGTTCAGCT CGCAGGACGA GGACGACGAG 
CCGCAGCAGC CGCAGCAACA ACCGCAGCCG TTGCAATCGC AGCAGCAGCA ACAGCAGCCG 
TTGCTGCAAA ATCCAACCAT CAATGGCCAA GTGCAGACGC AGCATTTCTA TCAGAATGCG 
CAGGAACTGC AGAAGGGCCA AAAGAACGGA TCCGTTCCGA TACTGGGACG CTGCAAGGCA 
CTGTATAGCT ACACCCCGAA GCTCTACGAC GAGCTGGAAC TGAGTCCCGG CGACATCATC 
GAGGTGCATG CCAAACAGGA CGACGGCTGG TGGCTGGGCG CCCTGCGCAA TCACATCGGC 
ATCTTTCCGG CCACCTATGT GGAGGAGTGC GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162712.1
CDS	451..2466
Translation	MGNAQGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_001169241.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant F (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169241.1

ORF Insert Sequence:

ATGGGTAATG CACAGGGACA GAACGGCTTC GAGGAACTCC GGCGATATGT GAAGCAGGGC 
GGTGATTTTA GCAAGGAGCT CATCTTTGTC CTGCAGGAGA GAGCCGATTC GGAGCTGATC 
TACTCCAAGT CGCTATCGAA ACTGGCCAAC AAGCTGAACA AAGCGGGCAG GGAAATTCCC 
GGGAGCGTGG CCGACGCCTG GCGTGGAGTG GCTACGGAGA TGGAGAGCCG CAGCGACATC 
CATCGCCAGT TGGCAGCCTC GCTAACGGAT GAGCTGGTCA AGCCGCTCAA GATCGTCGTC 
GAAGGACATC ACAAGGCGCG CAAGGCGGTG GAAAGCAACG TGGACAAGGC GGCGCGAGTA 
CTGGGCGAAT GGCGAGCCAG CGAGGCGAAA GCCAAGAAGG CCTCCCATAC GGCGGCGCGC 
GAGAACGAGA AGCTGCAGGA CGCAATGCTG GACGTGCGCA TCCAGAAGTC ACCATCGATT 
GCCCTGCTCC ACCAGGGACC CAACAAGCAG GCCGCAGAAA AGGAGCTCAA GTCAGCGGAG 
AAGGATTGCG TCAAGCTGGA TAACAAGCGC AAGAAGGCCG AGGAGGCGGT GAAGCGTGCG 
GATGTGGAAT ACTACACCCT GTGCGTGCGT TCGGAGCGAG CACGCGTAGA CTGGGAGATG 
GCCGTGCTCC GGGGAAGTGC CCAACTGCAG AGCAGCGAAC AGCAGCGGCT GGGCAATATG 
CACAACTTTG CCCAGCAGTA TGCACGCCTC ATCTCGGACA TGAATCCGAT ACTCGGTGGC 
CTGAGCACAC GCCTGCAGCC GCAACTCGAT GCTTGCAATG TGGCTAAGGA TATGCAAGTG 
GTGCGCCACA TCCGCCGCAA TTCGGAGGGA CCCAGTGAGC AACTCCTACC GGACTTTTAC 
TGCGAACACA CCACGCTGGC CATGAATCGG GAGCGTCGCA AGCACGCTCT GATCAAGCTG 
CTGCAGCTGG TCAAGACGGA TCTGGAGCGG GAGCGACGAT CCCGCGACGG ATTGAGGGGA 
CTGTCGCAAT CGCTTAACCA TCAGGAGCAT CAGAACATCA CCGATAAGCT GTACCACATT 
CGTTCCATGC TGACCTATTT GGAGGGCGCC CGTCTCAAGC TGCACTCTGC CCTCTTGGAG 
CTGGACCACA AGCCCAGAGC CACTCACCCA CTGGCCCAGC ACATTCATAT CACCCGTGAC 
CGCACTGGTT TGCAGCAGAG CATCCTGAAG GTGCCCAACT GGCTGAAGAA CAACGACAAG 
TCGCAGACGC AGCAATCGAC CAGTATGCTG GCCCACGACA TCACCGACAT AACCACTAAC 
CACGAGGATG AGCTTCCCGA CGAGAACTGC TCCCACCTGC ACAGCAGTAG CCATAGCAAC 
AATAATAGCA ACGGCTCCTG CACAGATGTC AGTTCGGTGG TGAAGCCTTT TAACCGAAGC 
AAAAGCAACA TCGAGACCAA TTTCACCAGT CAACCAAAGA TAATCTCGAC GACAAATGCC 
GCGATTGCCG TGTCCGTTAA GTCCAAAACC CTGGCCAATC TGCAGGATGG CCATCACAAT 
AACCAACAGC ACCATCATCA TCATCATAAT CATCACAGCG ATCGCGGCCA GGCGGATGGT 
GGATCCAATC AGCAGGACTC TGATTTCGAT GAGTTCAGCT CGCAGGACGA GGACGACGAG 
CCGCAGCAGC CGCAGCAACA ACCGCAGCCG TTGCAATCGC AGCAGCAGCA ACAGCAGCCG 
TTGCTGCAAA ATCCAACCAT CAATGGCCAA GTGCAGACGC AGCATTTCTA TCAGAATGCG 
CAGGAACTGC AGAAGGGCCA AAAGAACGGA TCCGTTCCGA TACTGGGACG CTGCAAGGCA 
CTGTATAGCT ACACCCCGAA GCTCTACGAC GAGCTGGAAC TGAGTCCCGG CGACATCATC 
GAGGTGCATG CCAAACAGGA CGACGGCTGG TGGCTGGGCG CCCTGCGCAA TCACATCGGC 
ATCTTTCCGG CCACCTATGT GGAGGAGTGC GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nost ( NM_132287.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572515.2 Drosophila melanogaster nostrin, transcript variant D (Nost), mRNA.

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CloneID	OFa03106
Clone ID Related Accession (Same CDS sequence)	NM_132287.3
Accession Version	NM_132287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_132287.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACAAT TCCGCGATAA TTCATGGGGA CAGAACGGCT TCGAGGAACT CCGGCGATAT 
GTGAAGCAGG GCGGTGATTT TAGCAAGGAG CTCATCTTTG TCCTGCAGGA GAGAGCCGAT 
TCGGAGCTGA TCTACTCCAA GTCGCTATCG AAACTGGCCA ACAAGCTGAA CAAAGCGGGC 
AGGGAAATTC CCGGGAGCGT GGCCGACGCC TGGCGTGGAG TGGCTACGGA GATGGAGAGC 
CGCAGCGACA TCCATCGCCA GTTGGCAGCC TCGCTAACGG ATGAGCTGGT CAAGCCGCTC 
AAGATCGTCG TCGAAGGACA TCACAAGGCG CGCAAGGCGG TGGAAAGCAA CGTGGACAAG 
GCGGCGCGAG TACTGGGCGA ATGGCGAGCC AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT 
ACGGCGGCGC GCGAGAACGA GAAGCTGCAG GACGCAATGC TGGACGTGCG CATCCAGAAG 
TCACCATCGA TTGCCCTGCT CCACCAGGGA CCCAACAAGC AGGCCGCAGA AAAGGAGCTC 
AAGTCAGCGG AGAAGGATTG CGTCAAGCTG GATAACAAGC GCAAGAAGGC CGAGGAGGCG 
GTGAAGCGTG CGGATGTGGA ATACTACACC CTGTGCGTGC GTTCGGAGCG AGCACGCGTA 
GACTGGGAGA TGGCCGTGCT CCGGGGAAGT GCCCAACTGC AGAGCAGCGA ACAGCAGCGG 
CTGGGCAATA TGCACAACTT TGCCCAGCAG TATGCACGCC TCATCTCGGA CATGAATCCG 
ATACTCGGTG GCCTGAGCAC ACGCCTGCAG CCGCAACTCG ATGCTTGCAA TGTGGCTAAG 
GATATGCAAG TGGTGCGCCA CATCCGCCGC AATTCGGAGG GACCCAGTGA GCAACTCCTA 
CCGGACTTTT ACTGCGAACA CACCACGCTG GCCATGAATC GGGAGCGTCG CAAGCACGCT 
CTGATCAAGC TGCTGCAGCT GGTCAAGACG GATCTGGAGC GGGAGCGACG ATCCCGCGAC 
GGATTGAGGG GACTGTCGCA ATCGCTTAAC CATCAGGAGC ATCAGAACAT CACCGATAAG 
CTGTACCACA TTCGTTCCAT GCTGACCTAT TTGGAGGGCG CCCGTCTCAA GCTGCACTCT 
GCCCTCTTGG AGCTGGACCA CAAGCCCAGA GCCACTCACC CACTGGCCCA GCACATTCAT 
ATCACCCGTG ACCGCACTGG TTTGCAGCAG AGCATCCTGA AGGTGCCCAA CTGGCTGAAG 
AACAACGACA AGTCGCAGAC GCAGCAATCG ACCAGTATGC TGGCCCACGA CATCACCGAC 
ATAACCACTA ACCACGAGGA TGAGCTTCCC GACGAGAACT GCTCCCACCT GCACAGCAGT 
AGCCATAGCA ACAATAATAG CAACGGCTCC TGCACAGATG TCAGTTCGGT GGTGAAGCCT 
TTTAACCGAA GCAAAAGCAA CATCGAGACC AATTTCACCA GTCAACCAAA GATAATCTCG 
ACGACAAATG CCGCGATTGC CGTGTCCGTT AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT 
GGCCATCACA ATAACCAACA GCACCATCAT CATCATCATA ATCATCACAG CGATCGCGGC 
CAGGCGGATG GTGGATCCAA TCAGCAGGAC TCTGATTTCG ATGAGTTCAG CTCGCAGGAC 
GAGGACGACG AGCCGCAGCA GCCGCAGCAA CAACCGCAGC CGTTGCAATC GCAGCAGCAG 
CAACAGCAGC CGTTGCTGCA AAATCCAACC ATCAATGGCC AAGTGCAGAC GCAGCATTTC 
TATCAGAATG CGCAGGAACT GCAGAAGGGC CAAAAGAACG GATCCGTTCC GATACTGGGA 
CGCTGCAAGG CACTGTATAG CTACACCCCG AAGCTCTACG ACGAGCTGGA ACTGAGTCCC 
GGCGACATCA TCGAGGTGCA TGCCAAACAG GACGACGGCT GGTGGCTGGG CGCCCTGCGC 
AATCACATCG GCATCTTTCC GGCCACCTAT GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572515.2
CDS	666..2693
Translation	MSQFRDNSWGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_132287.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant D (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132287.3

ORF Insert Sequence:

ATGTCACAAT TCCGCGATAA TTCATGGGGA CAGAACGGCT TCGAGGAACT CCGGCGATAT 
GTGAAGCAGG GCGGTGATTT TAGCAAGGAG CTCATCTTTG TCCTGCAGGA GAGAGCCGAT 
TCGGAGCTGA TCTACTCCAA GTCGCTATCG AAACTGGCCA ACAAGCTGAA CAAAGCGGGC 
AGGGAAATTC CCGGGAGCGT GGCCGACGCC TGGCGTGGAG TGGCTACGGA GATGGAGAGC 
CGCAGCGACA TCCATCGCCA GTTGGCAGCC TCGCTAACGG ATGAGCTGGT CAAGCCGCTC 
AAGATCGTCG TCGAAGGACA TCACAAGGCG CGCAAGGCGG TGGAAAGCAA CGTGGACAAG 
GCGGCGCGAG TACTGGGCGA ATGGCGAGCC AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT 
ACGGCGGCGC GCGAGAACGA GAAGCTGCAG GACGCAATGC TGGACGTGCG CATCCAGAAG 
TCACCATCGA TTGCCCTGCT CCACCAGGGA CCCAACAAGC AGGCCGCAGA AAAGGAGCTC 
AAGTCAGCGG AGAAGGATTG CGTCAAGCTG GATAACAAGC GCAAGAAGGC CGAGGAGGCG 
GTGAAGCGTG CGGATGTGGA ATACTACACC CTGTGCGTGC GTTCGGAGCG AGCACGCGTA 
GACTGGGAGA TGGCCGTGCT CCGGGGAAGT GCCCAACTGC AGAGCAGCGA ACAGCAGCGG 
CTGGGCAATA TGCACAACTT TGCCCAGCAG TATGCACGCC TCATCTCGGA CATGAATCCG 
ATACTCGGTG GCCTGAGCAC ACGCCTGCAG CCGCAACTCG ATGCTTGCAA TGTGGCTAAG 
GATATGCAAG TGGTGCGCCA CATCCGCCGC AATTCGGAGG GACCCAGTGA GCAACTCCTA 
CCGGACTTTT ACTGCGAACA CACCACGCTG GCCATGAATC GGGAGCGTCG CAAGCACGCT 
CTGATCAAGC TGCTGCAGCT GGTCAAGACG GATCTGGAGC GGGAGCGACG ATCCCGCGAC 
GGATTGAGGG GACTGTCGCA ATCGCTTAAC CATCAGGAGC ATCAGAACAT CACCGATAAG 
CTGTACCACA TTCGTTCCAT GCTGACCTAT TTGGAGGGCG CCCGTCTCAA GCTGCACTCT 
GCCCTCTTGG AGCTGGACCA CAAGCCCAGA GCCACTCACC CACTGGCCCA GCACATTCAT 
ATCACCCGTG ACCGCACTGG TTTGCAGCAG AGCATCCTGA AGGTGCCCAA CTGGCTGAAG 
AACAACGACA AGTCGCAGAC GCAGCAATCG ACCAGTATGC TGGCCCACGA CATCACCGAC 
ATAACCACTA ACCACGAGGA TGAGCTTCCC GACGAGAACT GCTCCCACCT GCACAGCAGT 
AGCCATAGCA ACAATAATAG CAACGGCTCC TGCACAGATG TCAGTTCGGT GGTGAAGCCT 
TTTAACCGAA GCAAAAGCAA CATCGAGACC AATTTCACCA GTCAACCAAA GATAATCTCG 
ACGACAAATG CCGCGATTGC CGTGTCCGTT AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT 
GGCCATCACA ATAACCAACA GCACCATCAT CATCATCATA ATCATCACAG CGATCGCGGC 
CAGGCGGATG GTGGATCCAA TCAGCAGGAC TCTGATTTCG ATGAGTTCAG CTCGCAGGAC 
GAGGACGACG AGCCGCAGCA GCCGCAGCAA CAACCGCAGC CGTTGCAATC GCAGCAGCAG 
CAACAGCAGC CGTTGCTGCA AAATCCAACC ATCAATGGCC AAGTGCAGAC GCAGCATTTC 
TATCAGAATG CGCAGGAACT GCAGAAGGGC CAAAAGAACG GATCCGTTCC GATACTGGGA 
CGCTGCAAGG CACTGTATAG CTACACCCCG AAGCTCTACG ACGAGCTGGA ACTGAGTCCC 
GGCGACATCA TCGAGGTGCA TGCCAAACAG GACGACGGCT GGTGGCTGGG CGCCCTGCGC 
AATCACATCG GCATCTTTCC GGCCACCTAT GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nost ( NM_001169240.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162711.1 Drosophila melanogaster nostrin, transcript variant E (Nost), mRNA.

Price & Availability↑

CloneID	OFa02659
Clone ID Related Accession (Same CDS sequence)	NM_001169240.2 , NM_001201638.2
Accession Version	NM_001169240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_001169240.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCACC TGCGGGTGCA CAAATTCAAG CTGGGCGGCA AAGATAATGC CGCCATGGCC 
GATTTGCCCC AATCGGAAAA CACCACCAAC AATAACAATA ATATCGGAAG CAATAATAAT 
CAAAAAGGCG GTGCAAAAGG ATTTCTGGGA AGGATTAAGC GCTACTCAGT GTTGGGAAAT 
AAACTGGGAC GTCGTAATCT CGGCAGTATG AATTTGAATC CGCCGAATAA TGTCGTTGAT 
AATTCCGTTT CGCCCACTAA ACTCCATACT GGAAGCTACA ATATGACCGG TAGCCATTCC 
GATGATCATC GCCTGGAAAT CGGAGCTCCG ATTTTAATAT CGACTACAAC ATTGGACACC 
GACCGTTTCG ATGTCACGGA GGCGAGACTC AAACAGATTG GCGGCGGCAT AGCCCAGACC 
TCCAGTATAG TAAGAACCCT GACGCCCAGG AGTTCGGATG AGGAGGAGTT TGTGGACGCC 
AGGAGTAGTC CGCAACAAAT GGAAAGGGAT GAGGATCTGC AAATCGCTGA TGATCAGGTC 
GAAAAGAACG ATCGCGATCA GTATGGTCAT CAATTTCAGC TTCCTAAGAA ACAACATCGT 
GAAGAAGAGA TGGGGGACTT AAAATATCTA GACGATCTGG AGGAACCAGA ACAATTTGAG 
ACGCCCAAAC ACGAGGTGGA GGAAAAGCTC CTGACTGCCA ACCAGGATGT GAAGCGCCCC 
ATAACGCCCA TAACCATTGC CATAGATCCC CCAATCAGAC GACCACGTAT TGCCCGCCAG 
CAAAGGCAAT CGCAATCCGT TCAGAATCTG CATCGCAGCG AACTGAAGGT ATACCTGCAC 
AAGTCCAACT CCGTGGCGCT GGACATGAAT GTCACGGCTC CGGCAAAGCT GGGCTTGGAC 
TTGAATCTGC CCGAGTTGCC GGAACTCTAT GGAGCCAGCG AGCTCAGTTT GGCGGCCAGT 
GATAATAAGG AAAATCTGCC AGCGGTGAAG CCATCCGCTG AGCGATCGCC CATACCAAAT 
GGTGGCTTCA TGGCCCAGCA GCCACATTTC AAGAGCCTAG ACTCGTTCCA CCTGAACACC 
CGCTCCCATT CGAATCAGAG CTCCCAACAG AGTTCCCGCC AAAGCTTGGC CTGCAGCAGC 
AGCAATGGGG AATACGACTT TGATCTGAAG TCGGTGAGCT ATCAGAGCCT GAATGCCCAG 
AATCTGTTCG TTTCGATTGA CGAACTGCAG GAGCTAACGC GTCAGATCAA CGAAACGGAG 
GATTTTGGCA AGGAGATCGA TCTGGAGTAC TGCGCGCATC GCGATCAATT GAGACCCAGT 
GAACGCAGGA TCACCCTGCT GAAGAACAAA AACCAAACGT TGATCAACTT CAATCACAAC 
AAGGAGAAGC TGCGTAAGGG CTGGCACGGC ATGAAGCACT GGCTGGGCGA GGAGGGCTCC 
AAGATTAAGG AGGCAGTGCG TCAACAGACG CCCCTCAAAC GTCTGGCCCA ATCACGGAGC 
AATCTTAACC AGTCAACTGC GGATGCCAGT CGCCAGTCAA TGTCGCCGGA ACGCACTCAA 
CGCGATGTAA CCGAGAGCTG TGAGGATGTC ACCGAACGCA CCGAAATGGA GTCGTCCATG 
TCGCACCGAA ACAGCGAGGA GGATCTGTCG CCGCATGCCA AGCGGTTCAA GGATGAGGGA 
CAGAACGGCT TCGAGGAACT CCGGCGATAT GTGAAGCAGG GCGGTGATTT TAGCAAGGAG 
CTCATCTTTG TCCTGCAGGA GAGAGCCGAT TCGGAGCTGA TCTACTCCAA GTCGCTATCG 
AAACTGGCCA ACAAGCTGAA CAAAGCGGGC AGGGAAATTC CCGGGAGCGT GGCCGACGCC 
TGGCGTGGAG TGGCTACGGA GATGGAGAGC CGCAGCGACA TCCATCGCCA GTTGGCAGCC 
TCGCTAACGG ATGAGCTGGT CAAGCCGCTC AAGATCGTCG TCGAAGGACA TCACAAGGCG 
CGCAAGGCGG TGGAAAGCAA CGTGGACAAG GCGGCGCGAG TACTGGGCGA ATGGCGAGCC 
AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT ACGGCGGCGC GCGAGAACGA GAAGCTGCAG 
GACGCAATGC TGGACGTGCG CATCCAGAAG TCACCATCGA TTGCCCTGCT CCACCAGGGA 
CCCAACAAGC AGGCCGCAGA AAAGGAGCTC AAGTCAGCGG AGAAGGATTG CGTCAAGCTG 
GATAACAAGC GCAAGAAGGC CGAGGAGGCG GTGAAGCGTG CGGATGTGGA ATACTACACC 
CTGTGCGTGC GTTCGGAGCG AGCACGCGTA GACTGGGAGA TGGCCGTGCT CCGGGGAAGT 
GCCCAACTGC AGAGCAGCGA ACAGCAGCGG CTGGGCAATA TGCACAACTT TGCCCAGCAG 
TATGCACGCC TCATCTCGGA CATGAATCCG ATACTCGGTG GCCTGAGCAC ACGCCTGCAG 
CCGCAACTCG ATGCTTGCAA TGTGGCTAAG GATATGCAAG TGGTGCGCCA CATCCGCCGC 
AATTCGGAGG GACCCAGTGA GCAACTCCTA CCGGACTTTT ACTGCGAACA CACCACGCTG 
GCCATGAATC GGGAGCGTCG CAAGCACGCT CTGATCAAGC TGCTGCAGCT GGTCAAGACG 
GATCTGGAGC GGGAGCGACG ATCCCGCGAC GGATTGAGGG GACTGTCGCA ATCGCTTAAC 
CATCAGGAGC ATCAGAACAT CACCGATAAG CTGTACCACA TTCGTTCCAT GCTGACCTAT 
TTGGAGGGCG CCCGTCTCAA GCTGCACTCT GCCCTCTTGG AGCTGGACCA CAAGCCCAGA 
GCCACTCACC CACTGGCCCA GCACATTCAT ATCACCCGTG ACCGCACTGG TTTGCAGCAG 
AGCATCCTGA AGGTGCCCAA CTGGCTGAAG AACAACGACA AGTCGCAGAC GCAGCAATCG 
ACCAGTATGC TGGCCCACGA CATCACCGAC ATAACCACTA ACCACGAGGA TGAGCTTCCC 
GACGAGAACT GCTCCCACCT GCACAGCAGT AGCCATAGCA ACAATAATAG CAACGGCTCC 
TGCACAGATG TCAGTTCGGT GGTGAAGCCT TTTAACCGAA GCAAAAGCAA CATCGAGACC 
AATTTCACCA GTCAACCAAA GATAATCTCG ACGACAAATG CCGCGATTGC CGTGTCCGTT 
AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT GGCCATCACA ATAACCAACA GCACCATCAT 
CATCATCATA ATCATCACAG CGATCGCGGC CAGGCGGATG GTGGATCCAA TCAGCAGGAC 
TCTGATTTCG ATGAGTTCAG CTCGCAGGAC GAGGACGACG AGCCGCAGCA GCCGCAGCAA 
CAACCGCAGC CGTTGCAATC GCAGCAGCAG CAACAGCAGC CGTTGCTGCA AAATCCAACC 
ATCAATGGCC AAGTGCAGAC GCAGCATTTC TATCAGAATG CGCAGGAACT GCAGAAGGGC 
CAAAAGAACG GATCCGTTCC GATACTGGGA CGCTGCAAGG CACTGTATAG CTACACCCCG 
AAGCTCTACG ACGAGCTGGA ACTGAGTCCC GGCGACATCA TCGAGGTGCA TGCCAAACAG 
GACGACGGCT GGTGGCTGGG CGCCCTGCGC AATCACATCG GCATCTTTCC GGCCACCTAT 
GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162711.1
CDS	197..3874
Translation	MNHLRVHKFKLGGKDNAAMADLPQSENTTNNNNNIGSNNNQKGGAKGFLGRIKRYSVLGNKLGRRNLGSMNLNPPNNVVDNSVSPTKLHTGSYNMTGSHSDDHRLEIGAPILISTTTLDTDRFDVTEARLKQIGGGIAQTSSIVRTLTPRSSDEEEFVDARSSPQQMERDEDLQIADDQVEKNDRDQYGHQFQLPKKQHREEEMGDLKYLDDLEEPEQFETPKHEVEEKLLTANQDVKRPITPITIAIDPPIRRPRIARQQRQSQSVQNLHRSELKVYLHKSNSVALDMNVTAPAKLGLDLNLPELPELYGASELSLAASDNKENLPAVKPSAERSPIPNGGFMAQQPHFKSLDSFHLNTRSHSNQSSQQSSRQSLACSSSNGEYDFDLKSVSYQSLNAQNLFVSIDELQELTRQINETEDFGKEIDLEYCAHRDQLRPSERRITLLKNKNQTLINFNHNKEKLRKGWHGMKHWLGEEGSKIKEAVRQQTPLKRLAQSRSNLNQSTADASRQSMSPERTQRDVTESCEDVTERTEMESSMSHRNSEEDLSPHAKRFKDEGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_001169240.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant E (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169240.2

ORF Insert Sequence:

ATGAACCACC TGCGGGTGCA CAAATTCAAG CTGGGCGGCA AAGATAATGC CGCCATGGCC 
GATTTGCCCC AATCGGAAAA CACCACCAAC AATAACAATA ATATCGGAAG CAATAATAAT 
CAAAAAGGCG GTGCAAAAGG ATTTCTGGGA AGGATTAAGC GCTACTCAGT GTTGGGAAAT 
AAACTGGGAC GTCGTAATCT CGGCAGTATG AATTTGAATC CGCCGAATAA TGTCGTTGAT 
AATTCCGTTT CGCCCACTAA ACTCCATACT GGAAGCTACA ATATGACCGG TAGCCATTCC 
GATGATCATC GCCTGGAAAT CGGAGCTCCG ATTTTAATAT CGACTACAAC ATTGGACACC 
GACCGTTTCG ATGTCACGGA GGCGAGACTC AAACAGATTG GCGGCGGCAT AGCCCAGACC 
TCCAGTATAG TAAGAACCCT GACGCCCAGG AGTTCGGATG AGGAGGAGTT TGTGGACGCC 
AGGAGTAGTC CGCAACAAAT GGAAAGGGAT GAGGATCTGC AAATCGCTGA TGATCAGGTC 
GAAAAGAACG ATCGCGATCA GTATGGTCAT CAATTTCAGC TTCCTAAGAA ACAACATCGT 
GAAGAAGAGA TGGGGGACTT AAAATATCTA GACGATCTGG AGGAACCAGA ACAATTTGAG 
ACGCCCAAAC ACGAGGTGGA GGAAAAGCTC CTGACTGCCA ACCAGGATGT GAAGCGCCCC 
ATAACGCCCA TAACCATTGC CATAGATCCC CCAATCAGAC GACCACGTAT TGCCCGCCAG 
CAAAGGCAAT CGCAATCCGT TCAGAATCTG CATCGCAGCG AACTGAAGGT ATACCTGCAC 
AAGTCCAACT CCGTGGCGCT GGACATGAAT GTCACGGCTC CGGCAAAGCT GGGCTTGGAC 
TTGAATCTGC CCGAGTTGCC GGAACTCTAT GGAGCCAGCG AGCTCAGTTT GGCGGCCAGT 
GATAATAAGG AAAATCTGCC AGCGGTGAAG CCATCCGCTG AGCGATCGCC CATACCAAAT 
GGTGGCTTCA TGGCCCAGCA GCCACATTTC AAGAGCCTAG ACTCGTTCCA CCTGAACACC 
CGCTCCCATT CGAATCAGAG CTCCCAACAG AGTTCCCGCC AAAGCTTGGC CTGCAGCAGC 
AGCAATGGGG AATACGACTT TGATCTGAAG TCGGTGAGCT ATCAGAGCCT GAATGCCCAG 
AATCTGTTCG TTTCGATTGA CGAACTGCAG GAGCTAACGC GTCAGATCAA CGAAACGGAG 
GATTTTGGCA AGGAGATCGA TCTGGAGTAC TGCGCGCATC GCGATCAATT GAGACCCAGT 
GAACGCAGGA TCACCCTGCT GAAGAACAAA AACCAAACGT TGATCAACTT CAATCACAAC 
AAGGAGAAGC TGCGTAAGGG CTGGCACGGC ATGAAGCACT GGCTGGGCGA GGAGGGCTCC 
AAGATTAAGG AGGCAGTGCG TCAACAGACG CCCCTCAAAC GTCTGGCCCA ATCACGGAGC 
AATCTTAACC AGTCAACTGC GGATGCCAGT CGCCAGTCAA TGTCGCCGGA ACGCACTCAA 
CGCGATGTAA CCGAGAGCTG TGAGGATGTC ACCGAACGCA CCGAAATGGA GTCGTCCATG 
TCGCACCGAA ACAGCGAGGA GGATCTGTCG CCGCATGCCA AGCGGTTCAA GGATGAGGGA 
CAGAACGGCT TCGAGGAACT CCGGCGATAT GTGAAGCAGG GCGGTGATTT TAGCAAGGAG 
CTCATCTTTG TCCTGCAGGA GAGAGCCGAT TCGGAGCTGA TCTACTCCAA GTCGCTATCG 
AAACTGGCCA ACAAGCTGAA CAAAGCGGGC AGGGAAATTC CCGGGAGCGT GGCCGACGCC 
TGGCGTGGAG TGGCTACGGA GATGGAGAGC CGCAGCGACA TCCATCGCCA GTTGGCAGCC 
TCGCTAACGG ATGAGCTGGT CAAGCCGCTC AAGATCGTCG TCGAAGGACA TCACAAGGCG 
CGCAAGGCGG TGGAAAGCAA CGTGGACAAG GCGGCGCGAG TACTGGGCGA ATGGCGAGCC 
AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT ACGGCGGCGC GCGAGAACGA GAAGCTGCAG 
GACGCAATGC TGGACGTGCG CATCCAGAAG TCACCATCGA TTGCCCTGCT CCACCAGGGA 
CCCAACAAGC AGGCCGCAGA AAAGGAGCTC AAGTCAGCGG AGAAGGATTG CGTCAAGCTG 
GATAACAAGC GCAAGAAGGC CGAGGAGGCG GTGAAGCGTG CGGATGTGGA ATACTACACC 
CTGTGCGTGC GTTCGGAGCG AGCACGCGTA GACTGGGAGA TGGCCGTGCT CCGGGGAAGT 
GCCCAACTGC AGAGCAGCGA ACAGCAGCGG CTGGGCAATA TGCACAACTT TGCCCAGCAG 
TATGCACGCC TCATCTCGGA CATGAATCCG ATACTCGGTG GCCTGAGCAC ACGCCTGCAG 
CCGCAACTCG ATGCTTGCAA TGTGGCTAAG GATATGCAAG TGGTGCGCCA CATCCGCCGC 
AATTCGGAGG GACCCAGTGA GCAACTCCTA CCGGACTTTT ACTGCGAACA CACCACGCTG 
GCCATGAATC GGGAGCGTCG CAAGCACGCT CTGATCAAGC TGCTGCAGCT GGTCAAGACG 
GATCTGGAGC GGGAGCGACG ATCCCGCGAC GGATTGAGGG GACTGTCGCA ATCGCTTAAC 
CATCAGGAGC ATCAGAACAT CACCGATAAG CTGTACCACA TTCGTTCCAT GCTGACCTAT 
TTGGAGGGCG CCCGTCTCAA GCTGCACTCT GCCCTCTTGG AGCTGGACCA CAAGCCCAGA 
GCCACTCACC CACTGGCCCA GCACATTCAT ATCACCCGTG ACCGCACTGG TTTGCAGCAG 
AGCATCCTGA AGGTGCCCAA CTGGCTGAAG AACAACGACA AGTCGCAGAC GCAGCAATCG 
ACCAGTATGC TGGCCCACGA CATCACCGAC ATAACCACTA ACCACGAGGA TGAGCTTCCC 
GACGAGAACT GCTCCCACCT GCACAGCAGT AGCCATAGCA ACAATAATAG CAACGGCTCC 
TGCACAGATG TCAGTTCGGT GGTGAAGCCT TTTAACCGAA GCAAAAGCAA CATCGAGACC 
AATTTCACCA GTCAACCAAA GATAATCTCG ACGACAAATG CCGCGATTGC CGTGTCCGTT 
AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT GGCCATCACA ATAACCAACA GCACCATCAT 
CATCATCATA ATCATCACAG CGATCGCGGC CAGGCGGATG GTGGATCCAA TCAGCAGGAC 
TCTGATTTCG ATGAGTTCAG CTCGCAGGAC GAGGACGACG AGCCGCAGCA GCCGCAGCAA 
CAACCGCAGC CGTTGCAATC GCAGCAGCAG CAACAGCAGC CGTTGCTGCA AAATCCAACC 
ATCAATGGCC AAGTGCAGAC GCAGCATTTC TATCAGAATG CGCAGGAACT GCAGAAGGGC 
CAAAAGAACG GATCCGTTCC GATACTGGGA CGCTGCAAGG CACTGTATAG CTACACCCCG 
AAGCTCTACG ACGAGCTGGA ACTGAGTCCC GGCGACATCA TCGAGGTGCA TGCCAAACAG 
GACGACGGCT GGTGGCTGGG CGCCCTGCGC AATCACATCG GCATCTTTCC GGCCACCTAT 
GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nost ( NM_001201638.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188567.1 Drosophila melanogaster nostrin, transcript variant G (Nost), mRNA.

Price & Availability↑

CloneID	OFa02659
Clone ID Related Accession (Same CDS sequence)	NM_001169240.2 , NM_001201638.2
Accession Version	NM_001201638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201638.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCACC TGCGGGTGCA CAAATTCAAG CTGGGCGGCA AAGATAATGC CGCCATGGCC 
GATTTGCCCC AATCGGAAAA CACCACCAAC AATAACAATA ATATCGGAAG CAATAATAAT 
CAAAAAGGCG GTGCAAAAGG ATTTCTGGGA AGGATTAAGC GCTACTCAGT GTTGGGAAAT 
AAACTGGGAC GTCGTAATCT CGGCAGTATG AATTTGAATC CGCCGAATAA TGTCGTTGAT 
AATTCCGTTT CGCCCACTAA ACTCCATACT GGAAGCTACA ATATGACCGG TAGCCATTCC 
GATGATCATC GCCTGGAAAT CGGAGCTCCG ATTTTAATAT CGACTACAAC ATTGGACACC 
GACCGTTTCG ATGTCACGGA GGCGAGACTC AAACAGATTG GCGGCGGCAT AGCCCAGACC 
TCCAGTATAG TAAGAACCCT GACGCCCAGG AGTTCGGATG AGGAGGAGTT TGTGGACGCC 
AGGAGTAGTC CGCAACAAAT GGAAAGGGAT GAGGATCTGC AAATCGCTGA TGATCAGGTC 
GAAAAGAACG ATCGCGATCA GTATGGTCAT CAATTTCAGC TTCCTAAGAA ACAACATCGT 
GAAGAAGAGA TGGGGGACTT AAAATATCTA GACGATCTGG AGGAACCAGA ACAATTTGAG 
ACGCCCAAAC ACGAGGTGGA GGAAAAGCTC CTGACTGCCA ACCAGGATGT GAAGCGCCCC 
ATAACGCCCA TAACCATTGC CATAGATCCC CCAATCAGAC GACCACGTAT TGCCCGCCAG 
CAAAGGCAAT CGCAATCCGT TCAGAATCTG CATCGCAGCG AACTGAAGGT ATACCTGCAC 
AAGTCCAACT CCGTGGCGCT GGACATGAAT GTCACGGCTC CGGCAAAGCT GGGCTTGGAC 
TTGAATCTGC CCGAGTTGCC GGAACTCTAT GGAGCCAGCG AGCTCAGTTT GGCGGCCAGT 
GATAATAAGG AAAATCTGCC AGCGGTGAAG CCATCCGCTG AGCGATCGCC CATACCAAAT 
GGTGGCTTCA TGGCCCAGCA GCCACATTTC AAGAGCCTAG ACTCGTTCCA CCTGAACACC 
CGCTCCCATT CGAATCAGAG CTCCCAACAG AGTTCCCGCC AAAGCTTGGC CTGCAGCAGC 
AGCAATGGGG AATACGACTT TGATCTGAAG TCGGTGAGCT ATCAGAGCCT GAATGCCCAG 
AATCTGTTCG TTTCGATTGA CGAACTGCAG GAGCTAACGC GTCAGATCAA CGAAACGGAG 
GATTTTGGCA AGGAGATCGA TCTGGAGTAC TGCGCGCATC GCGATCAATT GAGACCCAGT 
GAACGCAGGA TCACCCTGCT GAAGAACAAA AACCAAACGT TGATCAACTT CAATCACAAC 
AAGGAGAAGC TGCGTAAGGG CTGGCACGGC ATGAAGCACT GGCTGGGCGA GGAGGGCTCC 
AAGATTAAGG AGGCAGTGCG TCAACAGACG CCCCTCAAAC GTCTGGCCCA ATCACGGAGC 
AATCTTAACC AGTCAACTGC GGATGCCAGT CGCCAGTCAA TGTCGCCGGA ACGCACTCAA 
CGCGATGTAA CCGAGAGCTG TGAGGATGTC ACCGAACGCA CCGAAATGGA GTCGTCCATG 
TCGCACCGAA ACAGCGAGGA GGATCTGTCG CCGCATGCCA AGCGGTTCAA GGATGAGGGA 
CAGAACGGCT TCGAGGAACT CCGGCGATAT GTGAAGCAGG GCGGTGATTT TAGCAAGGAG 
CTCATCTTTG TCCTGCAGGA GAGAGCCGAT TCGGAGCTGA TCTACTCCAA GTCGCTATCG 
AAACTGGCCA ACAAGCTGAA CAAAGCGGGC AGGGAAATTC CCGGGAGCGT GGCCGACGCC 
TGGCGTGGAG TGGCTACGGA GATGGAGAGC CGCAGCGACA TCCATCGCCA GTTGGCAGCC 
TCGCTAACGG ATGAGCTGGT CAAGCCGCTC AAGATCGTCG TCGAAGGACA TCACAAGGCG 
CGCAAGGCGG TGGAAAGCAA CGTGGACAAG GCGGCGCGAG TACTGGGCGA ATGGCGAGCC 
AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT ACGGCGGCGC GCGAGAACGA GAAGCTGCAG 
GACGCAATGC TGGACGTGCG CATCCAGAAG TCACCATCGA TTGCCCTGCT CCACCAGGGA 
CCCAACAAGC AGGCCGCAGA AAAGGAGCTC AAGTCAGCGG AGAAGGATTG CGTCAAGCTG 
GATAACAAGC GCAAGAAGGC CGAGGAGGCG GTGAAGCGTG CGGATGTGGA ATACTACACC 
CTGTGCGTGC GTTCGGAGCG AGCACGCGTA GACTGGGAGA TGGCCGTGCT CCGGGGAAGT 
GCCCAACTGC AGAGCAGCGA ACAGCAGCGG CTGGGCAATA TGCACAACTT TGCCCAGCAG 
TATGCACGCC TCATCTCGGA CATGAATCCG ATACTCGGTG GCCTGAGCAC ACGCCTGCAG 
CCGCAACTCG ATGCTTGCAA TGTGGCTAAG GATATGCAAG TGGTGCGCCA CATCCGCCGC 
AATTCGGAGG GACCCAGTGA GCAACTCCTA CCGGACTTTT ACTGCGAACA CACCACGCTG 
GCCATGAATC GGGAGCGTCG CAAGCACGCT CTGATCAAGC TGCTGCAGCT GGTCAAGACG 
GATCTGGAGC GGGAGCGACG ATCCCGCGAC GGATTGAGGG GACTGTCGCA ATCGCTTAAC 
CATCAGGAGC ATCAGAACAT CACCGATAAG CTGTACCACA TTCGTTCCAT GCTGACCTAT 
TTGGAGGGCG CCCGTCTCAA GCTGCACTCT GCCCTCTTGG AGCTGGACCA CAAGCCCAGA 
GCCACTCACC CACTGGCCCA GCACATTCAT ATCACCCGTG ACCGCACTGG TTTGCAGCAG 
AGCATCCTGA AGGTGCCCAA CTGGCTGAAG AACAACGACA AGTCGCAGAC GCAGCAATCG 
ACCAGTATGC TGGCCCACGA CATCACCGAC ATAACCACTA ACCACGAGGA TGAGCTTCCC 
GACGAGAACT GCTCCCACCT GCACAGCAGT AGCCATAGCA ACAATAATAG CAACGGCTCC 
TGCACAGATG TCAGTTCGGT GGTGAAGCCT TTTAACCGAA GCAAAAGCAA CATCGAGACC 
AATTTCACCA GTCAACCAAA GATAATCTCG ACGACAAATG CCGCGATTGC CGTGTCCGTT 
AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT GGCCATCACA ATAACCAACA GCACCATCAT 
CATCATCATA ATCATCACAG CGATCGCGGC CAGGCGGATG GTGGATCCAA TCAGCAGGAC 
TCTGATTTCG ATGAGTTCAG CTCGCAGGAC GAGGACGACG AGCCGCAGCA GCCGCAGCAA 
CAACCGCAGC CGTTGCAATC GCAGCAGCAG CAACAGCAGC CGTTGCTGCA AAATCCAACC 
ATCAATGGCC AAGTGCAGAC GCAGCATTTC TATCAGAATG CGCAGGAACT GCAGAAGGGC 
CAAAAGAACG GATCCGTTCC GATACTGGGA CGCTGCAAGG CACTGTATAG CTACACCCCG 
AAGCTCTACG ACGAGCTGGA ACTGAGTCCC GGCGACATCA TCGAGGTGCA TGCCAAACAG 
GACGACGGCT GGTGGCTGGG CGCCCTGCGC AATCACATCG GCATCTTTCC GGCCACCTAT 
GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188567.1
CDS	158..3835
Translation	MNHLRVHKFKLGGKDNAAMADLPQSENTTNNNNNIGSNNNQKGGAKGFLGRIKRYSVLGNKLGRRNLGSMNLNPPNNVVDNSVSPTKLHTGSYNMTGSHSDDHRLEIGAPILISTTTLDTDRFDVTEARLKQIGGGIAQTSSIVRTLTPRSSDEEEFVDARSSPQQMERDEDLQIADDQVEKNDRDQYGHQFQLPKKQHREEEMGDLKYLDDLEEPEQFETPKHEVEEKLLTANQDVKRPITPITIAIDPPIRRPRIARQQRQSQSVQNLHRSELKVYLHKSNSVALDMNVTAPAKLGLDLNLPELPELYGASELSLAASDNKENLPAVKPSAERSPIPNGGFMAQQPHFKSLDSFHLNTRSHSNQSSQQSSRQSLACSSSNGEYDFDLKSVSYQSLNAQNLFVSIDELQELTRQINETEDFGKEIDLEYCAHRDQLRPSERRITLLKNKNQTLINFNHNKEKLRKGWHGMKHWLGEEGSKIKEAVRQQTPLKRLAQSRSNLNQSTADASRQSMSPERTQRDVTESCEDVTERTEMESSMSHRNSEEDLSPHAKRFKDEGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_001201638.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant G (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201638.2

ORF Insert Sequence:

ATGAACCACC TGCGGGTGCA CAAATTCAAG CTGGGCGGCA AAGATAATGC CGCCATGGCC 
GATTTGCCCC AATCGGAAAA CACCACCAAC AATAACAATA ATATCGGAAG CAATAATAAT 
CAAAAAGGCG GTGCAAAAGG ATTTCTGGGA AGGATTAAGC GCTACTCAGT GTTGGGAAAT 
AAACTGGGAC GTCGTAATCT CGGCAGTATG AATTTGAATC CGCCGAATAA TGTCGTTGAT 
AATTCCGTTT CGCCCACTAA ACTCCATACT GGAAGCTACA ATATGACCGG TAGCCATTCC 
GATGATCATC GCCTGGAAAT CGGAGCTCCG ATTTTAATAT CGACTACAAC ATTGGACACC 
GACCGTTTCG ATGTCACGGA GGCGAGACTC AAACAGATTG GCGGCGGCAT AGCCCAGACC 
TCCAGTATAG TAAGAACCCT GACGCCCAGG AGTTCGGATG AGGAGGAGTT TGTGGACGCC 
AGGAGTAGTC CGCAACAAAT GGAAAGGGAT GAGGATCTGC AAATCGCTGA TGATCAGGTC 
GAAAAGAACG ATCGCGATCA GTATGGTCAT CAATTTCAGC TTCCTAAGAA ACAACATCGT 
GAAGAAGAGA TGGGGGACTT AAAATATCTA GACGATCTGG AGGAACCAGA ACAATTTGAG 
ACGCCCAAAC ACGAGGTGGA GGAAAAGCTC CTGACTGCCA ACCAGGATGT GAAGCGCCCC 
ATAACGCCCA TAACCATTGC CATAGATCCC CCAATCAGAC GACCACGTAT TGCCCGCCAG 
CAAAGGCAAT CGCAATCCGT TCAGAATCTG CATCGCAGCG AACTGAAGGT ATACCTGCAC 
AAGTCCAACT CCGTGGCGCT GGACATGAAT GTCACGGCTC CGGCAAAGCT GGGCTTGGAC 
TTGAATCTGC CCGAGTTGCC GGAACTCTAT GGAGCCAGCG AGCTCAGTTT GGCGGCCAGT 
GATAATAAGG AAAATCTGCC AGCGGTGAAG CCATCCGCTG AGCGATCGCC CATACCAAAT 
GGTGGCTTCA TGGCCCAGCA GCCACATTTC AAGAGCCTAG ACTCGTTCCA CCTGAACACC 
CGCTCCCATT CGAATCAGAG CTCCCAACAG AGTTCCCGCC AAAGCTTGGC CTGCAGCAGC 
AGCAATGGGG AATACGACTT TGATCTGAAG TCGGTGAGCT ATCAGAGCCT GAATGCCCAG 
AATCTGTTCG TTTCGATTGA CGAACTGCAG GAGCTAACGC GTCAGATCAA CGAAACGGAG 
GATTTTGGCA AGGAGATCGA TCTGGAGTAC TGCGCGCATC GCGATCAATT GAGACCCAGT 
GAACGCAGGA TCACCCTGCT GAAGAACAAA AACCAAACGT TGATCAACTT CAATCACAAC 
AAGGAGAAGC TGCGTAAGGG CTGGCACGGC ATGAAGCACT GGCTGGGCGA GGAGGGCTCC 
AAGATTAAGG AGGCAGTGCG TCAACAGACG CCCCTCAAAC GTCTGGCCCA ATCACGGAGC 
AATCTTAACC AGTCAACTGC GGATGCCAGT CGCCAGTCAA TGTCGCCGGA ACGCACTCAA 
CGCGATGTAA CCGAGAGCTG TGAGGATGTC ACCGAACGCA CCGAAATGGA GTCGTCCATG 
TCGCACCGAA ACAGCGAGGA GGATCTGTCG CCGCATGCCA AGCGGTTCAA GGATGAGGGA 
CAGAACGGCT TCGAGGAACT CCGGCGATAT GTGAAGCAGG GCGGTGATTT TAGCAAGGAG 
CTCATCTTTG TCCTGCAGGA GAGAGCCGAT TCGGAGCTGA TCTACTCCAA GTCGCTATCG 
AAACTGGCCA ACAAGCTGAA CAAAGCGGGC AGGGAAATTC CCGGGAGCGT GGCCGACGCC 
TGGCGTGGAG TGGCTACGGA GATGGAGAGC CGCAGCGACA TCCATCGCCA GTTGGCAGCC 
TCGCTAACGG ATGAGCTGGT CAAGCCGCTC AAGATCGTCG TCGAAGGACA TCACAAGGCG 
CGCAAGGCGG TGGAAAGCAA CGTGGACAAG GCGGCGCGAG TACTGGGCGA ATGGCGAGCC 
AGCGAGGCGA AAGCCAAGAA GGCCTCCCAT ACGGCGGCGC GCGAGAACGA GAAGCTGCAG 
GACGCAATGC TGGACGTGCG CATCCAGAAG TCACCATCGA TTGCCCTGCT CCACCAGGGA 
CCCAACAAGC AGGCCGCAGA AAAGGAGCTC AAGTCAGCGG AGAAGGATTG CGTCAAGCTG 
GATAACAAGC GCAAGAAGGC CGAGGAGGCG GTGAAGCGTG CGGATGTGGA ATACTACACC 
CTGTGCGTGC GTTCGGAGCG AGCACGCGTA GACTGGGAGA TGGCCGTGCT CCGGGGAAGT 
GCCCAACTGC AGAGCAGCGA ACAGCAGCGG CTGGGCAATA TGCACAACTT TGCCCAGCAG 
TATGCACGCC TCATCTCGGA CATGAATCCG ATACTCGGTG GCCTGAGCAC ACGCCTGCAG 
CCGCAACTCG ATGCTTGCAA TGTGGCTAAG GATATGCAAG TGGTGCGCCA CATCCGCCGC 
AATTCGGAGG GACCCAGTGA GCAACTCCTA CCGGACTTTT ACTGCGAACA CACCACGCTG 
GCCATGAATC GGGAGCGTCG CAAGCACGCT CTGATCAAGC TGCTGCAGCT GGTCAAGACG 
GATCTGGAGC GGGAGCGACG ATCCCGCGAC GGATTGAGGG GACTGTCGCA ATCGCTTAAC 
CATCAGGAGC ATCAGAACAT CACCGATAAG CTGTACCACA TTCGTTCCAT GCTGACCTAT 
TTGGAGGGCG CCCGTCTCAA GCTGCACTCT GCCCTCTTGG AGCTGGACCA CAAGCCCAGA 
GCCACTCACC CACTGGCCCA GCACATTCAT ATCACCCGTG ACCGCACTGG TTTGCAGCAG 
AGCATCCTGA AGGTGCCCAA CTGGCTGAAG AACAACGACA AGTCGCAGAC GCAGCAATCG 
ACCAGTATGC TGGCCCACGA CATCACCGAC ATAACCACTA ACCACGAGGA TGAGCTTCCC 
GACGAGAACT GCTCCCACCT GCACAGCAGT AGCCATAGCA ACAATAATAG CAACGGCTCC 
TGCACAGATG TCAGTTCGGT GGTGAAGCCT TTTAACCGAA GCAAAAGCAA CATCGAGACC 
AATTTCACCA GTCAACCAAA GATAATCTCG ACGACAAATG CCGCGATTGC CGTGTCCGTT 
AAGTCCAAAA CCCTGGCCAA TCTGCAGGAT GGCCATCACA ATAACCAACA GCACCATCAT 
CATCATCATA ATCATCACAG CGATCGCGGC CAGGCGGATG GTGGATCCAA TCAGCAGGAC 
TCTGATTTCG ATGAGTTCAG CTCGCAGGAC GAGGACGACG AGCCGCAGCA GCCGCAGCAA 
CAACCGCAGC CGTTGCAATC GCAGCAGCAG CAACAGCAGC CGTTGCTGCA AAATCCAACC 
ATCAATGGCC AAGTGCAGAC GCAGCATTTC TATCAGAATG CGCAGGAACT GCAGAAGGGC 
CAAAAGAACG GATCCGTTCC GATACTGGGA CGCTGCAAGG CACTGTATAG CTACACCCCG 
AAGCTCTACG ACGAGCTGGA ACTGAGTCCC GGCGACATCA TCGAGGTGCA TGCCAAACAG 
GACGACGGCT GGTGGCTGGG CGCCCTGCGC AATCACATCG GCATCTTTCC GGCCACCTAT 
GTGGAGGAGT GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nost ( NM_001298100.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285029.1 Drosophila melanogaster nostrin, transcript variant H (Nost), mRNA.

Price & Availability↑

CloneID	OFa22440
Clone ID Related Accession (Same CDS sequence)	NM_001298100.1
Accession Version	NM_001298100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nostrin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACAAT TCCGCGATAA TTCATGGCTC GCCAATGCGC GATCATACGG CGTTTTGAAG 
AGTTTGAGAA GCTCGGCACG TTATGCCAAG GCCAAATCCA AGTCGGCCAT TAATCTCAAT 
GCCCATCTGA AGAAGCAGCA GAAGCTGGAG GAAGGACATC CCGAAAAGAA GAGGCAGGAT 
AAGAAAATAC TCAGACAGAG TGTCAGTGAG GATCAGCCGA TCTATGCGAA TGTGGACGAG 
GTTATACCGA TCCATTTAGA ACCGGAAACC GGAACCGAAG TCGATGCTCT AATGGGTAAT 
GCACAGCCGT CAAAGATGAA CCACCTGCGG GTGCACAAAT TCAAGCTGGG CGGCAAAGAT 
AATGCCGCCA TGGCCGATTT GCCCCAATCG GAAAACACCA CCAACAATAA CAATAATATC 
GGAAGCAATA ATAATCAAAA AGGCGGTGCA AAAGGATTTC TGGGAAGGAT TAAGCGCTAC 
TCAGTGTTGG GAAATAAACT GGGACGTCGT AATCTCGGCA GTATGAATTT GAATCCGCCG 
AATAATGTCG TTGATAATTC CGTTTCGCCC ACTAAACTCC ATACTGGAAG CTACAATATG 
ACCGGTAGCC ATTCCGATGA TCATCGCCTG GAAATCGGAG CTCCGATTTT AATATCGACT 
ACAACATTGG ACACCGACCG TTTCGATGTC ACGGAGGCGA GACTCAAACA GATTGGCGGC 
GGCATAGCCC AGACCTCCAG TATAGTAAGA ACCCTGACGC CCAGGAGTTC GGATGAGGAG 
GAGTTTGTGG ACGCCAGGAG TAGTCCGCAA CAAATGGAAA GGGATGAGGA TCTGCAAATC 
GCTGATGATC AGGTCGAAAA GAACGATCGC GATCAGTATG GTCATCAATT TCAGCTTCCT 
AAGAAACAAC ATCGTGAAGA AGAGATGGGG GACTTAAAAT ATCTAGACGA TCTGGAGGAA 
CCAGAACAAT TTGAGACGCC CAAACACGAG GTGGAGGAAA AGCTCCTGAC TGCCAACCAG 
GATGTGAAGC GCCCCATAAC GCCCATAACC ATTGCCATAG ATCCCCCAAT CAGACGACCA 
CGTATTGCCC GCCAGCAAAG GCAATCGCAA TCCGTTCAGA ATCTGCATCG CAGCGAACTG 
AAGGTATACC TGCACAAGTC CAACTCCGTG GCGCTGGACA TGAATGTCAC GGCTCCGGCA 
AAGCTGGGCT TGGACTTGAA TCTGCCCGAG TTGCCGGAAC TCTATGGAGC CAGCGAGCTC 
AGTTTGGCGG CCAGTGATAA TAAGGAAAAT CTGCCAGCGG TGAAGCCATC CGCTGAGCGA 
TCGCCCATAC CAAATGGTGG CTTCATGGCC CAGCAGCCAC ATTTCAAGAG CCTAGACTCG 
TTCCACCTGA ACACCCGCTC CCATTCGAAT CAGAGCTCCC AACAGAGTTC CCGCCAAAGC 
TTGGCCTGCA GCAGCAGCAA TGGGGAATAC GACTTTGATC TGAAGTCGGT GAGCTATCAG 
AGCCTGAATG CCCAGAATCT GTTCGTTTCG ATTGACGAAC TGCAGGAGCT AACGCGTCAG 
ATCAACGAAA CGGAGGATTT TGGCAAGGAG ATCGATCTGG AGTACTGCGC GCATCGCGAT 
CAATTGAGAC CCAGTGAACG CAGGATCACC CTGCTGAAGA ACAAAAACCA AACGTTGATC 
AACTTCAATC ACAACAAGGA GAAGCTGCGT AAGGGCTGGC ACGGCATGAA GCACTGGCTG 
GGCGAGGAGG GCTCCAAGAT TAAGGAGGCA GTGCGTCAAC AGACGCCCCT CAAACGTCTG 
GCCCAATCAC GGAGCAATCT TAACCAGTCA ACTGCGGATG CCAGTCGCCA GTCAATGTCG 
CCGGAACGCA CTCAACGCGA TGTAACCGAG AGCTGTGAGG ATGTCACCGA ACGCACCGAA 
ATGGAGTCGT CCATGTCGCA CCGAAACAGC GAGGAGGATC TGTCGCCGCA TGCCAAGCGG 
TTCAAGGATG AGGGACAGAA CGGCTTCGAG GAACTCCGGC GATATGTGAA GCAGGGCGGT 
GATTTTAGCA AGGAGCTCAT CTTTGTCCTG CAGGAGAGAG CCGATTCGGA GCTGATCTAC 
TCCAAGTCGC TATCGAAACT GGCCAACAAG CTGAACAAAG CGGGCAGGGA AATTCCCGGG 
AGCGTGGCCG ACGCCTGGCG TGGAGTGGCT ACGGAGATGG AGAGCCGCAG CGACATCCAT 
CGCCAGTTGG CAGCCTCGCT AACGGATGAG CTGGTCAAGC CGCTCAAGAT CGTCGTCGAA 
GGACATCACA AGGCGCGCAA GGCGGTGGAA AGCAACGTGG ACAAGGCGGC GCGAGTACTG 
GGCGAATGGC GAGCCAGCGA GGCGAAAGCC AAGAAGGCCT CCCATACGGC GGCGCGCGAG 
AACGAGAAGC TGCAGGACGC AATGCTGGAC GTGCGCATCC AGAAGTCACC ATCGATTGCC 
CTGCTCCACC AGGGACCCAA CAAGCAGGCC GCAGAAAAGG AGCTCAAGTC AGCGGAGAAG 
GATTGCGTCA AGCTGGATAA CAAGCGCAAG AAGGCCGAGG AGGCGGTGAA GCGTGCGGAT 
GTGGAATACT ACACCCTGTG CGTGCGTTCG GAGCGAGCAC GCGTAGACTG GGAGATGGCC 
GTGCTCCGGG GAAGTGCCCA ACTGCAGAGC AGCGAACAGC AGCGGCTGGG CAATATGCAC 
AACTTTGCCC AGCAGTATGC ACGCCTCATC TCGGACATGA ATCCGATACT CGGTGGCCTG 
AGCACACGCC TGCAGCCGCA ACTCGATGCT TGCAATGTGG CTAAGGATAT GCAAGTGGTG 
CGCCACATCC GCCGCAATTC GGAGGGACCC AGTGAGCAAC TCCTACCGGA CTTTTACTGC 
GAACACACCA CGCTGGCCAT GAATCGGGAG CGTCGCAAGC ACGCTCTGAT CAAGCTGCTG 
CAGCTGGTCA AGACGGATCT GGAGCGGGAG CGACGATCCC GCGACGGATT GAGGGGACTG 
TCGCAATCGC TTAACCATCA GGAGCATCAG AACATCACCG ATAAGCTGTA CCACATTCGT 
TCCATGCTGA CCTATTTGGA GGGCGCCCGT CTCAAGCTGC ACTCTGCCCT CTTGGAGCTG 
GACCACAAGC CCAGAGCCAC TCACCCACTG GCCCAGCACA TTCATATCAC CCGTGACCGC 
ACTGGTTTGC AGCAGAGCAT CCTGAAGGTG CCCAACTGGC TGAAGAACAA CGACAAGTCG 
CAGACGCAGC AATCGACCAG TATGCTGGCC CACGACATCA CCGACATAAC CACTAACCAC 
GAGGATGAGC TTCCCGACGA GAACTGCTCC CACCTGCACA GCAGTAGCCA TAGCAACAAT 
AATAGCAACG GCTCCTGCAC AGATGTCAGT TCGGTGGTGA AGCCTTTTAA CCGAAGCAAA 
AGCAACATCG AGACCAATTT CACCAGTCAA CCAAAGATAA TCTCGACGAC AAATGCCGCG 
ATTGCCGTGT CCGTTAAGTC CAAAACCCTG GCCAATCTGC AGGATGGCCA TCACAATAAC 
CAACAGCACC ATCATCATCA TCATAATCAT CACAGCGATC GCGGCCAGGC GGATGGTGGA 
TCCAATCAGC AGGACTCTGA TTTCGATGAG TTCAGCTCGC AGGACGAGGA CGACGAGCCG 
CAGCAGCCGC AGCAACAACC GCAGCCGTTG CAATCGCAGC AGCAGCAACA GCAGCCGTTG 
CTGCAAAATC CAACCATCAA TGGCCAAGTG CAGACGCAGC ATTTCTATCA GAATGCGCAG 
GAACTGCAGA AGGGCCAAAA GAACGGATCC GTTCCGATAC TGGGACGCTG CAAGGCACTG 
TATAGCTACA CCCCGAAGCT CTACGACGAG CTGGAACTGA GTCCCGGCGA CATCATCGAG 
GTGCATGCCA AACAGGACGA CGGCTGGTGG CTGGGCGCCC TGCGCAATCA CATCGGCATC 
TTTCCGGCCA CCTATGTGGA GGAGTGCGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285029.1
CDS	666..4658
Translation	MSQFRDNSWLANARSYGVLKSLRSSARYAKAKSKSAINLNAHLKKQQKLEEGHPEKKRQDKKILRQSVSEDQPIYANVDEVIPIHLEPETGTEVDALMGNAQPSKMNHLRVHKFKLGGKDNAAMADLPQSENTTNNNNNIGSNNNQKGGAKGFLGRIKRYSVLGNKLGRRNLGSMNLNPPNNVVDNSVSPTKLHTGSYNMTGSHSDDHRLEIGAPILISTTTLDTDRFDVTEARLKQIGGGIAQTSSIVRTLTPRSSDEEEFVDARSSPQQMERDEDLQIADDQVEKNDRDQYGHQFQLPKKQHREEEMGDLKYLDDLEEPEQFETPKHEVEEKLLTANQDVKRPITPITIAIDPPIRRPRIARQQRQSQSVQNLHRSELKVYLHKSNSVALDMNVTAPAKLGLDLNLPELPELYGASELSLAASDNKENLPAVKPSAERSPIPNGGFMAQQPHFKSLDSFHLNTRSHSNQSSQQSSRQSLACSSSNGEYDFDLKSVSYQSLNAQNLFVSIDELQELTRQINETEDFGKEIDLEYCAHRDQLRPSERRITLLKNKNQTLINFNHNKEKLRKGWHGMKHWLGEEGSKIKEAVRQQTPLKRLAQSRSNLNQSTADASRQSMSPERTQRDVTESCEDVTERTEMESSMSHRNSEEDLSPHAKRFKDEGQNGFEELRRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIALLHQGPNKQAAEKELKSAEKDCVKLDNKRKKAEEAVKRADVEYYTLCVRSERARVDWEMAVLRGSAQLQSSEQQRLGNMHNFAQQYARLISDMNPILGGLSTRLQPQLDACNVAKDMQVVRHIRRNSEGPSEQLLPDFYCEHTTLAMNRERRKHALIKLLQLVKTDLERERRSRDGLRGLSQSLNHQEHQNITDKLYHIRSMLTYLEGARLKLHSALLELDHKPRATHPLAQHIHITRDRTGLQQSILKVPNWLKNNDKSQTQQSTSMLAHDITDITTNHEDELPDENCSHLHSSSHSNNNSNGSCTDVSSVVKPFNRSKSNIETNFTSQPKIISTTNAAIAVSVKSKTLANLQDGHHNNQQHHHHHHNHHSDRGQADGGSNQQDSDFDEFSSQDEDDEPQQPQQQPQPLQSQQQQQQPLLQNPTINGQVQTQHFYQNAQELQKGQKNGSVPILGRCKALYSYTPKLYDELELSPGDIIEVHAKQDDGWWLGALRNHIGIFPATYVEECA

Target ORF information:

RefSeq Version	NM_001298100.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nostrin, transcript variant H (Nost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298100.1

ORF Insert Sequence:

ATGTCACAAT TCCGCGATAA TTCATGGCTC GCCAATGCGC GATCATACGG CGTTTTGAAG 
AGTTTGAGAA GCTCGGCACG TTATGCCAAG GCCAAATCCA AGTCGGCCAT TAATCTCAAT 
GCCCATCTGA AGAAGCAGCA GAAGCTGGAG GAAGGACATC CCGAAAAGAA GAGGCAGGAT 
AAGAAAATAC TCAGACAGAG TGTCAGTGAG GATCAGCCGA TCTATGCGAA TGTGGACGAG 
GTTATACCGA TCCATTTAGA ACCGGAAACC GGAACCGAAG TCGATGCTCT AATGGGTAAT 
GCACAGCCGT CAAAGATGAA CCACCTGCGG GTGCACAAAT TCAAGCTGGG CGGCAAAGAT 
AATGCCGCCA TGGCCGATTT GCCCCAATCG GAAAACACCA CCAACAATAA CAATAATATC 
GGAAGCAATA ATAATCAAAA AGGCGGTGCA AAAGGATTTC TGGGAAGGAT TAAGCGCTAC 
TCAGTGTTGG GAAATAAACT GGGACGTCGT AATCTCGGCA GTATGAATTT GAATCCGCCG 
AATAATGTCG TTGATAATTC CGTTTCGCCC ACTAAACTCC ATACTGGAAG CTACAATATG 
ACCGGTAGCC ATTCCGATGA TCATCGCCTG GAAATCGGAG CTCCGATTTT AATATCGACT 
ACAACATTGG ACACCGACCG TTTCGATGTC ACGGAGGCGA GACTCAAACA GATTGGCGGC 
GGCATAGCCC AGACCTCCAG TATAGTAAGA ACCCTGACGC CCAGGAGTTC GGATGAGGAG 
GAGTTTGTGG ACGCCAGGAG TAGTCCGCAA CAAATGGAAA GGGATGAGGA TCTGCAAATC 
GCTGATGATC AGGTCGAAAA GAACGATCGC GATCAGTATG GTCATCAATT TCAGCTTCCT 
AAGAAACAAC ATCGTGAAGA AGAGATGGGG GACTTAAAAT ATCTAGACGA TCTGGAGGAA 
CCAGAACAAT TTGAGACGCC CAAACACGAG GTGGAGGAAA AGCTCCTGAC TGCCAACCAG 
GATGTGAAGC GCCCCATAAC GCCCATAACC ATTGCCATAG ATCCCCCAAT CAGACGACCA 
CGTATTGCCC GCCAGCAAAG GCAATCGCAA TCCGTTCAGA ATCTGCATCG CAGCGAACTG 
AAGGTATACC TGCACAAGTC CAACTCCGTG GCGCTGGACA TGAATGTCAC GGCTCCGGCA 
AAGCTGGGCT TGGACTTGAA TCTGCCCGAG TTGCCGGAAC TCTATGGAGC CAGCGAGCTC 
AGTTTGGCGG CCAGTGATAA TAAGGAAAAT CTGCCAGCGG TGAAGCCATC CGCTGAGCGA 
TCGCCCATAC CAAATGGTGG CTTCATGGCC CAGCAGCCAC ATTTCAAGAG CCTAGACTCG 
TTCCACCTGA ACACCCGCTC CCATTCGAAT CAGAGCTCCC AACAGAGTTC CCGCCAAAGC 
TTGGCCTGCA GCAGCAGCAA TGGGGAATAC GACTTTGATC TGAAGTCGGT GAGCTATCAG 
AGCCTGAATG CCCAGAATCT GTTCGTTTCG ATTGACGAAC TGCAGGAGCT AACGCGTCAG 
ATCAACGAAA CGGAGGATTT TGGCAAGGAG ATCGATCTGG AGTACTGCGC GCATCGCGAT 
CAATTGAGAC CCAGTGAACG CAGGATCACC CTGCTGAAGA ACAAAAACCA AACGTTGATC 
AACTTCAATC ACAACAAGGA GAAGCTGCGT AAGGGCTGGC ACGGCATGAA GCACTGGCTG 
GGCGAGGAGG GCTCCAAGAT TAAGGAGGCA GTGCGTCAAC AGACGCCCCT CAAACGTCTG 
GCCCAATCAC GGAGCAATCT TAACCAGTCA ACTGCGGATG CCAGTCGCCA GTCAATGTCG 
CCGGAACGCA CTCAACGCGA TGTAACCGAG AGCTGTGAGG ATGTCACCGA ACGCACCGAA 
ATGGAGTCGT CCATGTCGCA CCGAAACAGC GAGGAGGATC TGTCGCCGCA TGCCAAGCGG 
TTCAAGGATG AGGGACAGAA CGGCTTCGAG GAACTCCGGC GATATGTGAA GCAGGGCGGT 
GATTTTAGCA AGGAGCTCAT CTTTGTCCTG CAGGAGAGAG CCGATTCGGA GCTGATCTAC 
TCCAAGTCGC TATCGAAACT GGCCAACAAG CTGAACAAAG CGGGCAGGGA AATTCCCGGG 
AGCGTGGCCG ACGCCTGGCG TGGAGTGGCT ACGGAGATGG AGAGCCGCAG CGACATCCAT 
CGCCAGTTGG CAGCCTCGCT AACGGATGAG CTGGTCAAGC CGCTCAAGAT CGTCGTCGAA 
GGACATCACA AGGCGCGCAA GGCGGTGGAA AGCAACGTGG ACAAGGCGGC GCGAGTACTG 
GGCGAATGGC GAGCCAGCGA GGCGAAAGCC AAGAAGGCCT CCCATACGGC GGCGCGCGAG 
AACGAGAAGC TGCAGGACGC AATGCTGGAC GTGCGCATCC AGAAGTCACC ATCGATTGCC 
CTGCTCCACC AGGGACCCAA CAAGCAGGCC GCAGAAAAGG AGCTCAAGTC AGCGGAGAAG 
GATTGCGTCA AGCTGGATAA CAAGCGCAAG AAGGCCGAGG AGGCGGTGAA GCGTGCGGAT 
GTGGAATACT ACACCCTGTG CGTGCGTTCG GAGCGAGCAC GCGTAGACTG GGAGATGGCC 
GTGCTCCGGG GAAGTGCCCA ACTGCAGAGC AGCGAACAGC AGCGGCTGGG CAATATGCAC 
AACTTTGCCC AGCAGTATGC ACGCCTCATC TCGGACATGA ATCCGATACT CGGTGGCCTG 
AGCACACGCC TGCAGCCGCA ACTCGATGCT TGCAATGTGG CTAAGGATAT GCAAGTGGTG 
CGCCACATCC GCCGCAATTC GGAGGGACCC AGTGAGCAAC TCCTACCGGA CTTTTACTGC 
GAACACACCA CGCTGGCCAT GAATCGGGAG CGTCGCAAGC ACGCTCTGAT CAAGCTGCTG 
CAGCTGGTCA AGACGGATCT GGAGCGGGAG CGACGATCCC GCGACGGATT GAGGGGACTG 
TCGCAATCGC TTAACCATCA GGAGCATCAG AACATCACCG ATAAGCTGTA CCACATTCGT 
TCCATGCTGA CCTATTTGGA GGGCGCCCGT CTCAAGCTGC ACTCTGCCCT CTTGGAGCTG 
GACCACAAGC CCAGAGCCAC TCACCCACTG GCCCAGCACA TTCATATCAC CCGTGACCGC 
ACTGGTTTGC AGCAGAGCAT CCTGAAGGTG CCCAACTGGC TGAAGAACAA CGACAAGTCG 
CAGACGCAGC AATCGACCAG TATGCTGGCC CACGACATCA CCGACATAAC CACTAACCAC 
GAGGATGAGC TTCCCGACGA GAACTGCTCC CACCTGCACA GCAGTAGCCA TAGCAACAAT 
AATAGCAACG GCTCCTGCAC AGATGTCAGT TCGGTGGTGA AGCCTTTTAA CCGAAGCAAA 
AGCAACATCG AGACCAATTT CACCAGTCAA CCAAAGATAA TCTCGACGAC AAATGCCGCG 
ATTGCCGTGT CCGTTAAGTC CAAAACCCTG GCCAATCTGC AGGATGGCCA TCACAATAAC 
CAACAGCACC ATCATCATCA TCATAATCAT CACAGCGATC GCGGCCAGGC GGATGGTGGA 
TCCAATCAGC AGGACTCTGA TTTCGATGAG TTCAGCTCGC AGGACGAGGA CGACGAGCCG 
CAGCAGCCGC AGCAACAACC GCAGCCGTTG CAATCGCAGC AGCAGCAACA GCAGCCGTTG 
CTGCAAAATC CAACCATCAA TGGCCAAGTG CAGACGCAGC ATTTCTATCA GAATGCGCAG 
GAACTGCAGA AGGGCCAAAA GAACGGATCC GTTCCGATAC TGGGACGCTG CAAGGCACTG 
TATAGCTACA CCCCGAAGCT CTACGACGAG CTGGAACTGA GTCCCGGCGA CATCATCGAG 
GTGCATGCCA AACAGGACGA CGGCTGGTGG CTGGGCGCCC TGCGCAATCA CATCGGCATC 
TTTCCGGCCA CCTATGTGGA GGAGTGCGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nost cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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