CkIIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CkIIalpha
Entrez Gene ID	48448
Full Name	casein kinase IIalpha
Synonyms	0958/08,CG17520,CJIIalpha,CK II,CK-2,CK-II,CK-II alpha,CK2,CK2 alpha,CK2-alpha,CK2a,CK2alpha,CKII,CKII alpha,CKII-alpha,CKIIa,CKIIalfa,CKIIalpha,Cask-II-a,Ck2alpha,CkII,DmCK2alpha,DmCKIIalpha,Dmel\CG17520,Tik,alphaCK2,anon-WO02059370.53,ck2alpha,ckIIalpha,dCK2alpha,dCKII,dCKII alpha,l(3)S095808
General protein information	Preferred Names casein kinase IIalpha Names CG17520 gene product from transcript CG17520-RE CG17520-PA CG17520-PB CG17520-PC CG17520-PD CG17520-PE CG17520-PF CG17520-PG CkIIalpha-PA CkIIalpha-PB CkIIalpha-PC CkIIalpha-PD CkIIalpha-PE CkIIalpha-PF CkIIalpha-PG NAP1-kinase alpha subunit of drosophila casein kinase II casein kinase 2 casein kinase II casein kinase II alpha casein kinase II alpha subunit casein kinase II-alpha subunit lethal (3) S095808 protein kinase CK2 timekeeper
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80D1-80D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300223.1	NP_001287152.1	casein kinase IIalpha, isoform G
NM_001043159.3	NP_001036624.1	casein kinase IIalpha, isoform D
NM_080179.5	NP_524918.1	casein kinase IIalpha, isoform B
NM_168986.2	NP_730775.1	casein kinase IIalpha, isoform C
NM_001300222.1	NP_001287151.1	casein kinase IIalpha, isoform F
NM_001300224.1	NP_001287153.1	casein kinase IIalpha, isoform E
NM_168985.2	NP_730774.1	casein kinase IIalpha, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CSNK2A1	XP_005634959.1
Rattus norvegicus (Norway rat)	Csnk2a1	NP_446276.1
Drosophila melanogaster (fruit fly)	CkIIalpha	NP_730775.1
Saccharomyces cerevisiae (baker's yeast)	CKA2	NP_014704.1
Magnaporthe oryzae (rice blast fungus)	MGG_03696	XP_003716185.1
Mus musculus (house mouse)	Csnk2a1	NP_031814.2
Gallus gallus (chicken)	CSNK2A1	NP_001002242.1
Arabidopsis thaliana (thale cress)	AT2G23070	NP_179889.1
Xenopus tropicalis (tropical clawed frog)	LOC100498392	XP_004920495.1
Homo sapiens (human)	CSNK2A1	NP_808227.1
Danio rerio (zebrafish)	LOC100535532	XP_003199454.1
Eremothecium gossypii	AGOS_ADR204W	NP_984300.1
Neurospora crassa	NCU03124	XP_964983.2
Bos taurus (cattle)	CSNK2A1	NP_777060.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005569	XP_315576.4
Caenorhabditis elegans (roundworm)	kin-3	NP_492811.1
Pan troglodytes (chimpanzee)	CSNK2A1	XP_003953729.1
	CSNK2A1	XP_002798227.1
Kluyveromyces lactis	KLLA0F16467g	XP_455820.1
Schizosaccharomyces pombe (fission yeast)	cka1	NP_593642.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CK2alpha might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2alpha is a novel regulator of the active zone protein, BRP, in Drosophila.
Title: CK2? regulates the transcription of BRP in Drosophila.

Coexpressing CK2alpha and CK2beta increases Smo accumulation and induces ectopic Hh target gene expression.
Title: Casein kinase 2 promotes Hedgehog signaling by regulating both smoothened and Cubitus interruptus.

overexpression of Tik elicits ectopic bristles in N(+) flies and suppresses the retinal defects of the gain-of-function allele N(spl).
Title: Functional dissection of Timekeeper (Tik) implicates opposite roles for CK2 and PP2A during Drosophila neurogenesis.

Coimmunoprecipitation experiments revealed that the alpha-subunit of the protein kinase CK2 (CK2alpha) was associated with soluble Stellate.
Title: Genetically Derepressed Nucleoplasmic Stellate Protein in Spermatocytes of D. melanogaster interacts with the catalytic subunit of protein kinase 2 and carries histone-like lysine-methylated mark.

Results describe the isolation of temperature-sensitive alleles of Drosophila CK2alpha.
Title: Drosophila protein kinase CK2 is rendered temperature-sensitive by mutations of highly conserved residues flanking the activation segment.

The following CkIIalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CkIIalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20256	NM_001043159.3 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_080179.5 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_168986.2 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_001300224.1 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_168985.2 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_001300222.1 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20256	NM_001300223.1 Latest version!	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CkIIalpha ( NM_001300223.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287152.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_001300223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287152.1
CDS	221..1231
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_001300223.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300223.1

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_001043159.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036624.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_001043159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001043159.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036624.1
CDS	373..1383
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_001043159.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043159.3

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_080179.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524918.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_080179.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080179.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524918.1
CDS	353..1363
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_080179.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080179.5

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_168986.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730775.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_168986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168986.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730775.1
CDS	215..1225
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_168986.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168986.2

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_001300222.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287151.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_001300222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287151.1
CDS	273..1283
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_001300222.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300222.1

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_001300224.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287153.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_001300224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287153.1
CDS	845..1855
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_001300224.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300224.1

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIIalpha ( NM_168985.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730774.1 Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20256
Clone ID Related Accession (Same CDS sequence)	NM_001300223.1 , NM_001043159.3 , NM_080179.5 , NM_168986.2 , NM_001300222.1 , NM_001300224.1 , NM_168985.2
Accession Version	NM_168985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase IIalpha, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168985.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730774.1
CDS	328..1338
Translation	MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ

Target ORF information:

RefSeq Version	NM_168985.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase IIalpha (CkIIalpha), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168985.2

ORF Insert Sequence:

ATGACACTTC CTAGTGCGGC TCGCGTGTAC ACAGATGTCA ATGCGCACAA ACCGGATGAA 
TATTGGGACT ATGAAAATTA TGTGGTTGAT TGGGGCAATC AAGACGATTA TCAGTTGGTC 
CGTAAATTAG GCCGTGGAAA GTATTCTGAG GTCTTCGAGG CCATTAATAT TACGACCACG 
GAAAAGTGCG TTGTTAAAAT TCTGAAACCT GTTAAAAAAA AGAAGATAAA GCGTGAAATC 
AAAATTTTGG AGAACTTGCG TGGAGGAACT AATATAATAA CACTTTTAGC CGTTGTCAAG 
GACCCAGTTT CTCGAACACC AGCGTTGATT TTTGAGCACG TCAACAACAC AGATTTCAAG 
CAACTTTACC AAACATTAAC TGATTATGAG ATTCGTTACT ACTTGTTTGA GCTTCTTAAG 
GCACTTGACT ACTGCCACAG CATGGGAATA ATGCATCGTG ATGTAAAGCC CCACAATGTT 
ATGATAGATC ACGAAAATCG AAAATTGCGC CTTATAGATT GGGGACTTGC CGAATTTTAC 
CATCCTGGTC AAGAATATAA TGTTCGTGTG GCTTCGAGAT ACTTTAAAGG TCCCGAATTA 
CTGGTAGATT ACCAGATGTA TGATTACTCA CTGGATATGT GGTCACTAGG TTGTATGTTG 
GCGTCGATGA TATTCCGAAA AGAGCCATTT TTCCACGGAC ATGATAACTA TGATCAATTG 
GTACGCATTG CCAAGGTGCT GGGCACCGAA GAACTCTACG CATATTTGGA TAAATACAAT 
ATTGACCTCG ATCCAAGATT TCACGACATT CTACAGCGTC ACTCACGAAA GCGATGGGAA 
AGATTTGTCC ATTCTGACAA CCAACATCTA GTTTCTCCTG AAGCACTAGA CTTTCTTGAT 
AAACTTTTAC GCTATGATCA CGTTGATCGA CTCACAGCTC GCGAAGCAAT GGCCCATCCA 
TATTTCTTAC CTATTGTCAA TGGTCAAATG AATCCCAATA ATCAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CkIIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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