kn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kn
Entrez Gene ID	45318
Full Name	knot
Synonyms	CG10197,Col,Col/Kn,DmCOE,Dmel\CG10197,Kn,Knot,coe,col,collier
General protein information	Preferred Names knot Names CG10197 gene product from transcript CG10197-RC CG10197-PA CG10197-PB CG10197-PC CG10197-PD CG10197-PE Collier-Knot KNOT colier collier collier/knot early B cell factor kn-PA kn-PB kn-PC kn-PD kn-PE knotted
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51C2-51C3\|2-72.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274051.1	NP_001260980.1	knot, isoform E
NM_080074.4	NP_524813.2	knot, isoform A
NM_166070.3	NP_725419.2	knot, isoform B
NM_001103847.2	NP_001097317.1	knot, isoform C
NM_137146.3	NP_610990.2	knot, isoform D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ebf3	NP_001074071.1
Drosophila melanogaster (fruit fly)	kn	NP_610990.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011437	XP_317866.4
	LOC713536	XP_002805907.1
Rattus norvegicus (Norway rat)	Ebf3	NP_001101976.1
Xenopus tropicalis (tropical clawed frog)	ebf3	XP_004915876.1
Pan troglodytes (chimpanzee)	EBF3	XP_003312875.1
Homo sapiens (human)	EBF3	NP_001005463.1
Mus musculus (house mouse)	Ebf3	NP_001106886.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two independent sites of Collier/Early B Cell Factor expression, hematopoietic progenitors and the PSC, achieve control of hematopoiesis
Title: Two Independent Functions of Collier/Early B Cell Factor in the Control of Drosophila Blood Cell Homeostasis.

Col expression is required in the prohemocytes to prevent their differentiation.
Title: The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche.

Col direct control of expression of the transcription regulators apterous and eyes-absent is critical to specifying neuronal identities. cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity
Title: Genome-Wide Mapping of Collier In Vivo Binding Sites Highlights Its Hierarchical Position in Different Transcription Regulatory Networks.

The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively.
Title: Sensory-neuron subtype-specific transcriptional programs controlling dendrite morphogenesis: genome-wide analysis of Abrupt and Knot/Collier.

Collier controls hemocyte homeostasis via coordinate regulation of PSC cell number and PSC signaling to prohemocytes.
Title: Size control of the Drosophila hematopoietic niche by bone morphogenetic protein signaling reveals parallels with mammals.

The following kn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11390	NM_166070.3 Latest version!	Drosophila melanogaster knot (kn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11392	NM_137146.3 Latest version!	Drosophila melanogaster knot (kn), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11393	NM_080074.4 Latest version!	Drosophila melanogaster knot (kn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12363	NM_001274051.1 Latest version!	Drosophila melanogaster knot (kn), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11391	NM_001103847.2 Latest version!	Drosophila melanogaster knot (kn), transcript variant C, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kn ( NM_166070.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725419.2 Drosophila melanogaster knot (kn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11390
Clone ID Related Accession (Same CDS sequence)	NM_166070.3
Accession Version	NM_166070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knot, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166070.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCAG CTCTCAACGA ACCCACAATC GACTACGGTT TCCAGCGCCT GCAGAAGCTC 
ATTCCCCGGC ATCCCGGCGA TCCCGAGAAG CTCCAGAAAG AGATAATCCT CAAGAGGGCT 
GCCGATCTGG TCGAGGCGCT GTACTCCATG CCCAGATCTC CGGGCGGCTC GACGGGCTTC 
AATTCCTATG CCGGTCAACT GGCGGTCAGT GTCCAGGATG GCTCCGGCCA GTGGACCGAG 
GACGATTACC AACGGGCGCA GTCGAGCAGC GTGAGTCCAC GTGGTGGCTA CTGCAGCAGC 
GCCTCCACGC CGCACAGCTC GGGAGGATCC TACGGTGCCA CGGCGGCCAG TGCAGCGGTG 
GCAGCCACGG CCAATGGCTA TGCACCTGCA CCCAACATGG GCACACTCTC CTCGTCGCCC 
GGCAGCGTCT TCAATTCCAC GTCAATGTCC GCCGTGTCGT CGACGTGGCA CCAGGCGTTC 
GTGCAGCACC ACCACGCGGC AACGGCCCAC CCGCACCACC ACTACCCACA TCCACATCAG 
CCGTGGCACA ATCCGGCCGT GTCAGCAGCC ACGGCGGCGG CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725419.2
CDS	518..2245
Translation	MEWGRKLYPSAVSGPRSAGGLMFGLPPTAAVDMNQPRGPMTSLKEEPLGSRWAMQPVVDQSNLGIGRAHFEKQPPSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQEQDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVISTQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGTGNTSLSISGHPLAPDSTYDGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGTMLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSALNEPTIDYGFQRLQKLIPRHPGDPEKLQKEIILKRAADLVEALYSMPRSPGGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATAASAAVAATANGYAPAPNMGTLSSSPGSVFNSTSMSAVSSTWHQAFVQHHHAATAHPHHHYPHPHQPWHNPAVSAATAAAV

Target ORF information:

RefSeq Version	NM_166070.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knot (kn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166070.3

ORF Insert Sequence:

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCAG CTCTCAACGA ACCCACAATC GACTACGGTT TCCAGCGCCT GCAGAAGCTC 
ATTCCCCGGC ATCCCGGCGA TCCCGAGAAG CTCCAGAAAG AGATAATCCT CAAGAGGGCT 
GCCGATCTGG TCGAGGCGCT GTACTCCATG CCCAGATCTC CGGGCGGCTC GACGGGCTTC 
AATTCCTATG CCGGTCAACT GGCGGTCAGT GTCCAGGATG GCTCCGGCCA GTGGACCGAG 
GACGATTACC AACGGGCGCA GTCGAGCAGC GTGAGTCCAC GTGGTGGCTA CTGCAGCAGC 
GCCTCCACGC CGCACAGCTC GGGAGGATCC TACGGTGCCA CGGCGGCCAG TGCAGCGGTG 
GCAGCCACGG CCAATGGCTA TGCACCTGCA CCCAACATGG GCACACTCTC CTCGTCGCCC 
GGCAGCGTCT TCAATTCCAC GTCAATGTCC GCCGTGTCGT CGACGTGGCA CCAGGCGTTC 
GTGCAGCACC ACCACGCGGC AACGGCCCAC CCGCACCACC ACTACCCACA TCCACATCAG 
CCGTGGCACA ATCCGGCCGT GTCAGCAGCC ACGGCGGCGG CCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kn ( NM_137146.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610990.2 Drosophila melanogaster knot (kn), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11392
Clone ID Related Accession (Same CDS sequence)	NM_137146.3
Accession Version	NM_137146.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knot, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137146.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCTC TCAACGAACC CACAATCGAC TACGGTTTCC AGCGCCTGCA GAAGCTCATT 
CCCCGGCATC CCGGCGATCC CGAGAAGCTC CAGAAAGAGA TAATCCTCAA GAGGGCTGCC 
GATCTGGTCG AGGCGCTGTA CTCCATGCCC AGATCTCCGG GCGGCTCGAC GGGCTTCAAT 
TCCTATGCCG GTCAACTGGC GGTCAGTGTC CAGGATGGCT CCGGCCAGTG GACCGAGGAC 
GATTACCAAC GGGCGCAGTC GAGCAGCGTG AGTCCACGTG GTGGCTACTG CAGCAGCGCC 
TCCACGCCGC ACAGCTCGGG AGGATCCTAC GGTGCCACGG CGGCCAGTGC AGCGGTGGCA 
GCCACGGCCA ATGGCTATGC ACCTGCACCC AACATGGGCA CACTCTCCTC GTCGCCCGGC 
AGCGTCTTCA ATTCCACGTC AAGGGTCAGC AGCCTGAGCT TCAATCCCTT CGCCCTGCCC 
ACCTGCAATA CACAGGGCTA TAGCACCCAA CTGGTGACGT CAACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610990.2
CDS	518..2188
Translation	MEWGRKLYPSAVSGPRSAGGLMFGLPPTAAVDMNQPRGPMTSLKEEPLGSRWAMQPVVDQSNLGIGRAHFEKQPPSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQEQDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVISTQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGTGNTSLSISGHPLAPDSTYDGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGTMLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSLNEPTIDYGFQRLQKLIPRHPGDPEKLQKEIILKRAADLVEALYSMPRSPGGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATAASAAVAATANGYAPAPNMGTLSSSPGSVFNSTSRVSSLSFNPFALPTCNTQGYSTQLVTSTK

Target ORF information:

RefSeq Version	NM_137146.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knot (kn), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137146.3

ORF Insert Sequence:

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCTC TCAACGAACC CACAATCGAC TACGGTTTCC AGCGCCTGCA GAAGCTCATT 
CCCCGGCATC CCGGCGATCC CGAGAAGCTC CAGAAAGAGA TAATCCTCAA GAGGGCTGCC 
GATCTGGTCG AGGCGCTGTA CTCCATGCCC AGATCTCCGG GCGGCTCGAC GGGCTTCAAT 
TCCTATGCCG GTCAACTGGC GGTCAGTGTC CAGGATGGCT CCGGCCAGTG GACCGAGGAC 
GATTACCAAC GGGCGCAGTC GAGCAGCGTG AGTCCACGTG GTGGCTACTG CAGCAGCGCC 
TCCACGCCGC ACAGCTCGGG AGGATCCTAC GGTGCCACGG CGGCCAGTGC AGCGGTGGCA 
GCCACGGCCA ATGGCTATGC ACCTGCACCC AACATGGGCA CACTCTCCTC GTCGCCCGGC 
AGCGTCTTCA ATTCCACGTC AAGGGTCAGC AGCCTGAGCT TCAATCCCTT CGCCCTGCCC 
ACCTGCAATA CACAGGGCTA TAGCACCCAA CTGGTGACGT CAACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kn ( NM_080074.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524813.2 Drosophila melanogaster knot (kn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11393
Clone ID Related Accession (Same CDS sequence)	NM_080074.4
Accession Version	NM_080074.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knot, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080074.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCAG CTCTCAACGA ACCCACAATC GACTACGGTT TCCAGCGCCT GCAGAAGCTC 
ATTCCCCGGC ATCCCGGCGA TCCCGAGAAG CTCCAGAAAG AGATAATCCT CAAGAGGGCT 
GCCGATCTGG TCGAGGCGCT GTACTCCATG CCCAGATCTC CGGGCGGCTC GACGGGCTTC 
AATTCCTATG CCGGTCAACT GGCGGTCAGT GTCCAGGATG GCTCCGGCCA GTGGACCGAG 
GACGATTACC AACGGGCGCA GTCGAGCAGC GTGAGTCCAC GTGGTGGCTA CTGCAGCAGC 
GCCTCCACGC CGCACAGCTC GGGAGGATCC TACGGTGCCA CGGCGGCCAG TGCAGCGGTG 
GCAGCCACGG CCAATGGCTA TGCACCTGCA CCCAACATGG GCACACTCTC CTCGTCGCCC 
GGCAGCGTCT TCAATTCCAC GTCAAGGGTC AGCAGCCTGA GCTTCAATCC CTTCGCCCTG 
CCCACCTGCA ATACACAGGG CTATAGCACC CAACTGGTGA CGTCAACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524813.2
CDS	518..2191
Translation	MEWGRKLYPSAVSGPRSAGGLMFGLPPTAAVDMNQPRGPMTSLKEEPLGSRWAMQPVVDQSNLGIGRAHFEKQPPSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQEQDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVISTQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGTGNTSLSISGHPLAPDSTYDGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGTMLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSALNEPTIDYGFQRLQKLIPRHPGDPEKLQKEIILKRAADLVEALYSMPRSPGGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATAASAAVAATANGYAPAPNMGTLSSSPGSVFNSTSRVSSLSFNPFALPTCNTQGYSTQLVTSTK

Target ORF information:

RefSeq Version	NM_080074.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knot (kn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080074.4

ORF Insert Sequence:

ATGGAGTGGG GCCGGAAGCT GTACCCCAGT GCCGTTTCCG GTCCGCGATC CGCCGGCGGC 
CTGATGTTCG GCCTGCCGCC CACCGCCGCT GTGGACATGA ACCAGCCGCG CGGTCCGATG 
ACCTCGCTGA AGGAGGAGCC GCTGGGCAGC CGGTGGGCCA TGCAGCCAGT CGTTGACCAG 
AGCAATTTGG GCATTGGCCG CGCCCACTTT GAAAAGCAGC CGCCCAGCAA TTTGCGCAAG 
TCGAACTTCT TTCACTTCGT GATCGCCTTA TATGATCGGG CTGGACAACC GATCGAAATC 
GAGCGGACGG CTTTCATTGG ATTCATCGAG AAGGACTCGG AATCGGATGC CACCAAGACG 
AACAATGGCA TCCAGTACCG GCTGCAGTTA CTCTACGCAA ATGGAGCTCG CCAGGAGCAG 
GACATTTTCG TGCGTCTCAT CGATTCGGTG ACCAAGCAGG CCATCATATA TGAGGGTCAG 
GACAAGAATC CCGAGATGTG TCGAGTGCTC CTAACGCACG AGGTGATGTG CAGCCGCTGC 
TGTGATAAGA AGAGCTGTGG TAACCGCAAC GAGACGCCAT CGGATCCCGT CATTATTGAT 
CGCTTCTTCC TGAAATTCTT CTTGAAATGC AATCAAAACT GTCTGAAAAA CGCTGGCAAT 
CCGCGGGATA TGCGCCGATT TCAGGTGGTA ATTTCTACGC AGGTGGCCGT GGATGGACCA 
CTATTGGCCA TCTCCGACAA CATGTTCGTG CATAACAATT CGAAGCACGG ACGGAGGGCC 
AAGCGGCTGG ACACCACGGA AGGTACAGGC AACACATCCC TGTCCATTTC CGGTCACCCC 
CTAGCGCCCG ACAGTACCTA CGATGGTCTC TACCCACCGC TGCCAGTGGC CACGCCCTGC 
ATCAAGGCGA TCTCGCCCAG CGAAGGCTGG ACAACCGGTG GCGCCACCGT GATCATAGTG 
GGCGACAACT TCTTCGATGG CCTGCAGGTT GTGTTCGGCA CCATGCTGGT CTGGAGCGAG 
CTAATTACCT CGCATGCGAT CCGGGTGCAG ACCCCGCCAA GGCACATCCC CGGCGTGGTG 
GAGGTGACGC TATCCTATAA GAGCAAACAG TTCTGCAAGG GATCACCCGG TCGCTTCGTC 
TATGTCTCAG CTCTCAACGA ACCCACAATC GACTACGGTT TCCAGCGCCT GCAGAAGCTC 
ATTCCCCGGC ATCCCGGCGA TCCCGAGAAG CTCCAGAAAG AGATAATCCT CAAGAGGGCT 
GCCGATCTGG TCGAGGCGCT GTACTCCATG CCCAGATCTC CGGGCGGCTC GACGGGCTTC 
AATTCCTATG CCGGTCAACT GGCGGTCAGT GTCCAGGATG GCTCCGGCCA GTGGACCGAG 
GACGATTACC AACGGGCGCA GTCGAGCAGC GTGAGTCCAC GTGGTGGCTA CTGCAGCAGC 
GCCTCCACGC CGCACAGCTC GGGAGGATCC TACGGTGCCA CGGCGGCCAG TGCAGCGGTG 
GCAGCCACGG CCAATGGCTA TGCACCTGCA CCCAACATGG GCACACTCTC CTCGTCGCCC 
GGCAGCGTCT TCAATTCCAC GTCAAGGGTC AGCAGCCTGA GCTTCAATCC CTTCGCCCTG 
CCCACCTGCA ATACACAGGG CTATAGCACC CAACTGGTGA CGTCAACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kn ( NM_001274051.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260980.1 Drosophila melanogaster knot (kn), transcript variant E, mRNA.

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CloneID	OFa12363
Clone ID Related Accession (Same CDS sequence)	NM_001274051.1
Accession Version	NM_001274051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knot, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGCGG GATTTGTTCA TTCAGCGGCG GCAAATCAGC ACTCCCATCC ACACGGACAC 
CCCCCACATC ACCATCTCGA TAATCACATC CAGAGGCAGG ACAATCCTAA TCAGAACCAT 
AATCACAATC AGACATTAGA CAATAAGACA ATGCGCTGTG ATACGAGTTT GGGCATTGGC 
CGCGCCCACT TTGAAAAGCA GCCGCCCAGC AATTTGCGCA AGTCGAACTT CTTTCACTTC 
GTGATCGCCT TATATGATCG GGCTGGACAA CCGATCGAAA TCGAGCGGAC GGCTTTCATT 
GGATTCATCG AGAAGGACTC GGAATCGGAT GCCACCAAGA CGAACAATGG CATCCAGTAC 
CGGCTGCAGT TACTCTACGC AAATGGAGCT CGCCAGGAGC AGGACATTTT CGTGCGTCTC 
ATCGATTCGG TGACCAAGCA GGCCATCATA TATGAGGGTC AGGACAAGAA TCCCGAGATG 
TGTCGAGTGC TCCTAACGCA CGAGGTGATG TGCAGCCGCT GCTGTGATAA GAAGAGCTGT 
GGTAACCGCA ACGAGACGCC ATCGGATCCC GTCATTATTG ATCGCTTCTT CCTGAAATTC 
TTCTTGAAAT GCAATCAAAA CTGTCTGAAA AACGCTGGCA ATCCGCGGGA TATGCGCCGA 
TTTCAGGTGG CCGTGGATGG ACCACTATTG GCCATCTCCG ACAACATGTT CGTGCATAAC 
AATTCGAAGC ACGGACGGAG GGCCAAGCGG CTGGACACCA CGGAAGGTCT CTACCCACCG 
CTGCCAGTGG CCACGCCCTG CATCAAGGCG ATCTCGCCCA GCGAAGGCTG GACAACCGGT 
GGCGCCACCG TGATCATAGT GGGCGACAAC TTCTTCGATG GCCTGCAGGT TGTGTTCGGC 
ACCATGCTGG TCTGGAGCGA GCTAATTACC TCGCATGCGA TCCGGGTGCA GACCCCGCCA 
AGGCACATCC CCGGCGTGGT GGAGGTGACG CTATCCTATA AGAGCAAACA GTTCTGCAAG 
GGATCACCCG GTCGCTTCGT CTATGTCTCT CTCAACGAAC CCACAATCGA CTACGGTTTC 
CAGCGCCTGC AGAAGCTCAT TCCCCGGCAT CCCGGCGATC CCGAGAAGCT CCAGAAAGAG 
ATAATCCTCA AGAGGGCTGC CGATCTGGTC GAGGCGCTGT ACTCCATGCC CAGCAGATCT 
CCGGGCGGCT CGACGGGCTT CAATTCCTAT GCCGGTCAAC TGGCGGTCAG TGTCCAGGAT 
GGCTCCGGCC AGTGGACCGA GGACGATTAC CAACGGGCGC AGTCGAGCAG CGTGAGTCCA 
CGTGGTGGCT ACTGCAGCAG CGCCTCCACG CCGCACAGCT CGGGAGGATC CTACGGTGCC 
ACGGCGGCCA GTGCAGCGGT GGCAGCCACG GCCAATGGCT ATGCACCTGC ACCCAACATG 
GGCACACTCT CCTCGTCGCC CGGCAGCGTC TTCAATTCCA CGTCAAGGGT CAGCAGCCTG 
AGCTTCAATC CCTTCGCCCT GCCCACCTGC AATACACAGG GCTATAGCAC CCAACTGGTG 
ACGTCAACCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260980.1
CDS	84..1658
Translation	MTAGFVHSAAANQHSHPHGHPPHHHLDNHIQRQDNPNQNHNHNQTLDNKTMRCDTSLGIGRAHFEKQPPSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQEQDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGTMLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSLNEPTIDYGFQRLQKLIPRHPGDPEKLQKEIILKRAADLVEALYSMPSRSPGGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATAASAAVAATANGYAPAPNMGTLSSSPGSVFNSTSRVSSLSFNPFALPTCNTQGYSTQLVTSTK

Target ORF information:

RefSeq Version	NM_001274051.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knot (kn), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274051.1

ORF Insert Sequence:

ATGACAGCGG GATTTGTTCA TTCAGCGGCG GCAAATCAGC ACTCCCATCC ACACGGACAC 
CCCCCACATC ACCATCTCGA TAATCACATC CAGAGGCAGG ACAATCCTAA TCAGAACCAT 
AATCACAATC AGACATTAGA CAATAAGACA ATGCGCTGTG ATACGAGTTT GGGCATTGGC 
CGCGCCCACT TTGAAAAGCA GCCGCCCAGC AATTTGCGCA AGTCGAACTT CTTTCACTTC 
GTGATCGCCT TATATGATCG GGCTGGACAA CCGATCGAAA TCGAGCGGAC GGCTTTCATT 
GGATTCATCG AGAAGGACTC GGAATCGGAT GCCACCAAGA CGAACAATGG CATCCAGTAC 
CGGCTGCAGT TACTCTACGC AAATGGAGCT CGCCAGGAGC AGGACATTTT CGTGCGTCTC 
ATCGATTCGG TGACCAAGCA GGCCATCATA TATGAGGGTC AGGACAAGAA TCCCGAGATG 
TGTCGAGTGC TCCTAACGCA CGAGGTGATG TGCAGCCGCT GCTGTGATAA GAAGAGCTGT 
GGTAACCGCA ACGAGACGCC ATCGGATCCC GTCATTATTG ATCGCTTCTT CCTGAAATTC 
TTCTTGAAAT GCAATCAAAA CTGTCTGAAA AACGCTGGCA ATCCGCGGGA TATGCGCCGA 
TTTCAGGTGG CCGTGGATGG ACCACTATTG GCCATCTCCG ACAACATGTT CGTGCATAAC 
AATTCGAAGC ACGGACGGAG GGCCAAGCGG CTGGACACCA CGGAAGGTCT CTACCCACCG 
CTGCCAGTGG CCACGCCCTG CATCAAGGCG ATCTCGCCCA GCGAAGGCTG GACAACCGGT 
GGCGCCACCG TGATCATAGT GGGCGACAAC TTCTTCGATG GCCTGCAGGT TGTGTTCGGC 
ACCATGCTGG TCTGGAGCGA GCTAATTACC TCGCATGCGA TCCGGGTGCA GACCCCGCCA 
AGGCACATCC CCGGCGTGGT GGAGGTGACG CTATCCTATA AGAGCAAACA GTTCTGCAAG 
GGATCACCCG GTCGCTTCGT CTATGTCTCT CTCAACGAAC CCACAATCGA CTACGGTTTC 
CAGCGCCTGC AGAAGCTCAT TCCCCGGCAT CCCGGCGATC CCGAGAAGCT CCAGAAAGAG 
ATAATCCTCA AGAGGGCTGC CGATCTGGTC GAGGCGCTGT ACTCCATGCC CAGCAGATCT 
CCGGGCGGCT CGACGGGCTT CAATTCCTAT GCCGGTCAAC TGGCGGTCAG TGTCCAGGAT 
GGCTCCGGCC AGTGGACCGA GGACGATTAC CAACGGGCGC AGTCGAGCAG CGTGAGTCCA 
CGTGGTGGCT ACTGCAGCAG CGCCTCCACG CCGCACAGCT CGGGAGGATC CTACGGTGCC 
ACGGCGGCCA GTGCAGCGGT GGCAGCCACG GCCAATGGCT ATGCACCTGC ACCCAACATG 
GGCACACTCT CCTCGTCGCC CGGCAGCGTC TTCAATTCCA CGTCAAGGGT CAGCAGCCTG 
AGCTTCAATC CCTTCGCCCT GCCCACCTGC AATACACAGG GCTATAGCAC CCAACTGGTG 
ACGTCAACCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kn ( NM_001103847.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097317.1 Drosophila melanogaster knot (kn), transcript variant C, partial mRNA.

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CloneID	OFa11391
Clone ID Related Accession (Same CDS sequence)	NM_001103847.2
Accession Version	NM_001103847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knot, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_001103847.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGTGACAG AGCAAAATGC TGACCGCCGC CTACTAGGAA AAATAATCCC CAGCCGATTT 
GAACGATTTA TTATTTGGCC CAGCCAACTC GGAGCCTGTC CCGGAATACC CGGAACCATT 
AATCATCCGA GTCCAGATCC AAGTCCGAGT CCGAGTGGTG GACAGGCGGC ATTGCAATTG 
GTGGACATTT TGGAGTGGAA GAAACTTTTG CGCGCAGGAT TAGGAGGAGA CGTAGCCGCG 
TACGCTGATC TCCACCAGCA CTCGCATTCG CACCATCCGA ATCCGCATCC ACATCCGCAT 
CCCCATCCGT TCGCCACCGA CGGCTTCAAG CTGCGTTCCG AAGAGCCGGT GCCGGGCTTC 
AGCTCGGCCT CCCCCTGGCC CACCCTGGAG CTGGGCATGG AGTGGGGCCG GAAGCTGTAC 
CCCAGTGCCG TTTCCGGTCC GCGATCCGCC GGCGGCCTGA TGTTCGGCCT GCCGCCCACC 
GCCGCTGTGG ACATGAACCA GCCGCGCGGT CCGATGACCT CGCTGAAGGA GGAGCCGCTG 
GGCAGCCGGT GGGCCATGCA GCCAGTCGTT GACCAGAGCA ATTTGGGCAT TGGCCGCGCC 
CACTTTGAAA AGCAGCCGCC CAGCAATTTG CGCAAGTCGA ACTTCTTTCA CTTCGTGATC 
GCCTTATATG ATCGGGCTGG ACAACCGATC GAAATCGAGC GGACGGCTTT CATTGGATTC 
ATCGAGAAGG ACTCGGAATC GGATGCCACC AAGACGAACA ATGGCATCCA GTACCGGCTG 
CAGTTACTCT ACGCAAATGG AGCTCGCCAG GAGCAGGACA TTTTCGTGCG TCTCATCGAT 
TCGGTGACCA AGCAGGCCAT CATATATGAG GGTCAGGACA AGAATCCCGA GATGTGTCGA 
GTGCTCCTAA CGCACGAGGT GATGTGCAGC CGCTGCTGTG ATAAGAAGAG CTGTGGTAAC 
CGCAACGAGA CGCCATCGGA TCCCGTCATT ATTGATCGCT TCTTCCTGAA ATTCTTCTTG 
AAATGCAATC AAAACTGTCT GAAAAACGCT GGCAATCCGC GGGATATGCG CCGATTTCAG 
GTGGTAATTT CTACGCAGGT GGCCGTGGAT GGACCACTAT TGGCCATCTC CGACAACATG 
TTCGTGCATA ACAATTCGAA GCACGGACGG AGGGCCAAGC GGCTGGACAC CACGGAAGGT 
ACAGGCAACA CATCCCTGTC CATTTCCGGT CACCCCCTAG CGCCCGACAG TACCTACGAT 
GGTCTCTACC CACCGCTGCC AGTGGCCACG CCCTGCATCA AGGCGATCTC GCCCAGCGAA 
GGCTGGACAA CCGGTGGCGC CACCGTGATC ATAGTGGGCG ACAACTTCTT CGATGGCCTG 
CAGGTTGTGT TCGGCACCAT GCTGGTCTGG AGCGAGCTAA TTACCTCGCA TGCGATCCGG 
GTGCAGACCC CGCCAAGGCA CATCCCCGGC GTGGTGGAGG TGACGCTATC CTATAAGAGC 
AAACAGTTCT GCAAGGGATC ACCCGGTCGC TTCGTCTATG TCTCAGCTCT CAACGAACCC 
ACAATCGACT ACGGTTTCCA GCGCCTGCAG AAGCTCATTC CCCGGCATCC CGGCGATCCC 
GAGAAGCTCC AGAAAGAGAT AATCCTCAAG AGGGCTGCCG ATCTGGTCGA GGCGCTGTAC 
TCCATGCCCA GATCTCCGGG CGGCTCGACG GGCTTCAATT CCTATGCCGG TCAACTGGCG 
GTCAGTGTCC AGGATGGCTC CGGCCAGTGG ACCGAGGACG ATTACCAACG GGCGCAGTCG 
AGCAGCGTGA GTCCACGTGG TGGCTACTGC AGCAGCGCCT CCACGCCGCA CAGCTCGGGA 
GGATCCTACG GTGCCACGGC GGCCAGTGCA GCGGTGGCAG CCACGGCCAA TGGCTATGCA 
CCTGCACCCA ACATGGGCAC ACTCTCCTCG TCGCCCGGCA GCGTCTTCAA TTCCACGTCA 
AGGGTCAGCA GCCTGAGCTT CAATCCCTTC GCCCTGCCCA CCTGCAATAC ACAGGGCTAT 
AGCACCCAAC TGGTGACGTC AACCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097317.1
CDS	1..2070
Translation	MVTEQNADRRLLGKIIPSRFERFIIWPSQLGACPGIPGTINHPSPDPSPSPSGGQAALQLVDILEWKKLLRAGLGGDVAAYADLHQHSHSHHPNPHPHPHPHPFATDGFKLRSEEPVPGFSSASPWPTLELGMEWGRKLYPSAVSGPRSAGGLMFGLPPTAAVDMNQPRGPMTSLKEEPLGSRWAMQPVVDQSNLGIGRAHFEKQPPSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQEQDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVISTQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGTGNTSLSISGHPLAPDSTYDGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGTMLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSALNEPTIDYGFQRLQKLIPRHPGDPEKLQKEIILKRAADLVEALYSMPRSPGGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATAASAAVAATANGYAPAPNMGTLSSSPGSVFNSTSRVSSLSFNPFALPTCNTQGYSTQLVTSTK

Target ORF information:

RefSeq Version	NM_001103847.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knot (kn), transcript variant C, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103847.2

ORF Insert Sequence:

ATGGTGACAG AGCAAAATGC TGACCGCCGC CTACTAGGAA AAATAATCCC CAGCCGATTT 
GAACGATTTA TTATTTGGCC CAGCCAACTC GGAGCCTGTC CCGGAATACC CGGAACCATT 
AATCATCCGA GTCCAGATCC AAGTCCGAGT CCGAGTGGTG GACAGGCGGC ATTGCAATTG 
GTGGACATTT TGGAGTGGAA GAAACTTTTG CGCGCAGGAT TAGGAGGAGA CGTAGCCGCG 
TACGCTGATC TCCACCAGCA CTCGCATTCG CACCATCCGA ATCCGCATCC ACATCCGCAT 
CCCCATCCGT TCGCCACCGA CGGCTTCAAG CTGCGTTCCG AAGAGCCGGT GCCGGGCTTC 
AGCTCGGCCT CCCCCTGGCC CACCCTGGAG CTGGGCATGG AGTGGGGCCG GAAGCTGTAC 
CCCAGTGCCG TTTCCGGTCC GCGATCCGCC GGCGGCCTGA TGTTCGGCCT GCCGCCCACC 
GCCGCTGTGG ACATGAACCA GCCGCGCGGT CCGATGACCT CGCTGAAGGA GGAGCCGCTG 
GGCAGCCGGT GGGCCATGCA GCCAGTCGTT GACCAGAGCA ATTTGGGCAT TGGCCGCGCC 
CACTTTGAAA AGCAGCCGCC CAGCAATTTG CGCAAGTCGA ACTTCTTTCA CTTCGTGATC 
GCCTTATATG ATCGGGCTGG ACAACCGATC GAAATCGAGC GGACGGCTTT CATTGGATTC 
ATCGAGAAGG ACTCGGAATC GGATGCCACC AAGACGAACA ATGGCATCCA GTACCGGCTG 
CAGTTACTCT ACGCAAATGG AGCTCGCCAG GAGCAGGACA TTTTCGTGCG TCTCATCGAT 
TCGGTGACCA AGCAGGCCAT CATATATGAG GGTCAGGACA AGAATCCCGA GATGTGTCGA 
GTGCTCCTAA CGCACGAGGT GATGTGCAGC CGCTGCTGTG ATAAGAAGAG CTGTGGTAAC 
CGCAACGAGA CGCCATCGGA TCCCGTCATT ATTGATCGCT TCTTCCTGAA ATTCTTCTTG 
AAATGCAATC AAAACTGTCT GAAAAACGCT GGCAATCCGC GGGATATGCG CCGATTTCAG 
GTGGTAATTT CTACGCAGGT GGCCGTGGAT GGACCACTAT TGGCCATCTC CGACAACATG 
TTCGTGCATA ACAATTCGAA GCACGGACGG AGGGCCAAGC GGCTGGACAC CACGGAAGGT 
ACAGGCAACA CATCCCTGTC CATTTCCGGT CACCCCCTAG CGCCCGACAG TACCTACGAT 
GGTCTCTACC CACCGCTGCC AGTGGCCACG CCCTGCATCA AGGCGATCTC GCCCAGCGAA 
GGCTGGACAA CCGGTGGCGC CACCGTGATC ATAGTGGGCG ACAACTTCTT CGATGGCCTG 
CAGGTTGTGT TCGGCACCAT GCTGGTCTGG AGCGAGCTAA TTACCTCGCA TGCGATCCGG 
GTGCAGACCC CGCCAAGGCA CATCCCCGGC GTGGTGGAGG TGACGCTATC CTATAAGAGC 
AAACAGTTCT GCAAGGGATC ACCCGGTCGC TTCGTCTATG TCTCAGCTCT CAACGAACCC 
ACAATCGACT ACGGTTTCCA GCGCCTGCAG AAGCTCATTC CCCGGCATCC CGGCGATCCC 
GAGAAGCTCC AGAAAGAGAT AATCCTCAAG AGGGCTGCCG ATCTGGTCGA GGCGCTGTAC 
TCCATGCCCA GATCTCCGGG CGGCTCGACG GGCTTCAATT CCTATGCCGG TCAACTGGCG 
GTCAGTGTCC AGGATGGCTC CGGCCAGTGG ACCGAGGACG ATTACCAACG GGCGCAGTCG 
AGCAGCGTGA GTCCACGTGG TGGCTACTGC AGCAGCGCCT CCACGCCGCA CAGCTCGGGA 
GGATCCTACG GTGCCACGGC GGCCAGTGCA GCGGTGGCAG CCACGGCCAA TGGCTATGCA 
CCTGCACCCA ACATGGGCAC ACTCTCCTCG TCGCCCGGCA GCGTCTTCAA TTCCACGTCA 
AGGGTCAGCA GCCTGAGCTT CAATCCCTTC GCCCTGCCCA CCTGCAATAC ACAGGGCTAT 
AGCACCCAAC TGGTGACGTC AACCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kn cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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