Mkrn1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mkrn1
Entrez Gene ID	44131
Full Name	Makorin 1
Synonyms	CG7184,Dmel\CG7184,MKRN1
General protein information	Preferred Names Makorin 1 Names CG7184 gene product from transcript CG7184-RA CG7184-PA CG7184-PB CG7184-PC Mkrn1-PA Mkrn1-PB Mkrn1-PC makorin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D5-78D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259938.2	NP_001246867.1	makorin 1, isoform C
NM_079965.4	NP_524704.1	makorin 1, isoform A
NM_168922.3	NP_730653.1	makorin 1, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MKRN1	NP_990073.1
Drosophila melanogaster (fruit fly)	Mkrn1	NP_001246867.1
Drosophila melanogaster (fruit fly)	CG5347	NP_572970.1
Xenopus tropicalis (tropical clawed frog)	mkrn1	XP_004912513.1
Mus musculus (house mouse)	Mkrn1	NP_061280.2
Homo sapiens (human)	MKRN1	NP_038474.2
Drosophila melanogaster (fruit fly)	CG5334	NP_572969.1
Caenorhabditis elegans (roundworm)	Y55F3AM.6	NP_001023510.1
Pan troglodytes (chimpanzee)	MKRN1	XP_519424.2
	MKRN1	XP_001107668.2
Danio rerio (zebrafish)	mkrn1	NP_694510.1
Canis lupus familiaris (dog)	MKRN1	XP_851764.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mkrn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mkrn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19368	NM_001259938.2 Latest version!	Drosophila melanogaster makorin 1, transcript variant C (Mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19368	NM_079965.4 Latest version!	Drosophila melanogaster makorin 1, transcript variant A (Mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19368	NM_168922.3 Latest version!	Drosophila melanogaster makorin 1, transcript variant B (Mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mkrn1 ( NM_001259938.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246867.1 Drosophila melanogaster makorin 1, transcript variant C (Mkrn1), mRNA.

Price & Availability↑

CloneID	OFa19368
Clone ID Related Accession (Same CDS sequence)	NM_001259938.2 , NM_079965.4 , NM_168922.3
Accession Version	NM_001259938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	makorin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259938.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246867.1
CDS	206..1366
Translation	MSAVTSSDTAISGMALGRSQTICRYYVRGICRFGELCRFSHDLSRGRPECEEQVATDVLPKPSTSSSSTIGSRSASISSQQRNWANAPVFVPSQKRYTAHEQSEFETTVDPEAVMEAQAGASYDTLAPGVSWAEVVGGPSSLNKEDYGEENSSCAWGEFSAYPIHMELCEMCDQYCLHPTDQVQRRSHNRECLQQHEQAMELSFAIARSKDKTCGICFDTIMEKAGREKRFGILPNCNHIFCLECIRTWRQAKQFENKITRACPECRVCSDFVCPSAFWMETKEEKDKLLNDYRAALGAKDCKYFKKGEGKCPFGNKCFYKHALPNGDIVDVGLPKRTRKLQSQNEIIDLLDIYLWDYVDRRDYHWLEMISSDITSSESSDYSDED

Target ORF information:

RefSeq Version	NM_001259938.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster makorin 1, transcript variant C (Mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259938.2

ORF Insert Sequence:

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkrn1 ( NM_079965.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524704.1 Drosophila melanogaster makorin 1, transcript variant A (Mkrn1), mRNA.

Price & Availability↑

CloneID	OFa19368
Clone ID Related Accession (Same CDS sequence)	NM_001259938.2 , NM_079965.4 , NM_168922.3
Accession Version	NM_079965.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	makorin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079965.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524704.1
CDS	214..1374
Translation	MSAVTSSDTAISGMALGRSQTICRYYVRGICRFGELCRFSHDLSRGRPECEEQVATDVLPKPSTSSSSTIGSRSASISSQQRNWANAPVFVPSQKRYTAHEQSEFETTVDPEAVMEAQAGASYDTLAPGVSWAEVVGGPSSLNKEDYGEENSSCAWGEFSAYPIHMELCEMCDQYCLHPTDQVQRRSHNRECLQQHEQAMELSFAIARSKDKTCGICFDTIMEKAGREKRFGILPNCNHIFCLECIRTWRQAKQFENKITRACPECRVCSDFVCPSAFWMETKEEKDKLLNDYRAALGAKDCKYFKKGEGKCPFGNKCFYKHALPNGDIVDVGLPKRTRKLQSQNEIIDLLDIYLWDYVDRRDYHWLEMISSDITSSESSDYSDED

Target ORF information:

RefSeq Version	NM_079965.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster makorin 1, transcript variant A (Mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079965.4

ORF Insert Sequence:

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkrn1 ( NM_168922.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730653.1 Drosophila melanogaster makorin 1, transcript variant B (Mkrn1), mRNA.

Price & Availability↑

CloneID	OFa19368
Clone ID Related Accession (Same CDS sequence)	NM_001259938.2 , NM_079965.4 , NM_168922.3
Accession Version	NM_168922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	makorin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168922.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730653.1
CDS	140..1300
Translation	MSAVTSSDTAISGMALGRSQTICRYYVRGICRFGELCRFSHDLSRGRPECEEQVATDVLPKPSTSSSSTIGSRSASISSQQRNWANAPVFVPSQKRYTAHEQSEFETTVDPEAVMEAQAGASYDTLAPGVSWAEVVGGPSSLNKEDYGEENSSCAWGEFSAYPIHMELCEMCDQYCLHPTDQVQRRSHNRECLQQHEQAMELSFAIARSKDKTCGICFDTIMEKAGREKRFGILPNCNHIFCLECIRTWRQAKQFENKITRACPECRVCSDFVCPSAFWMETKEEKDKLLNDYRAALGAKDCKYFKKGEGKCPFGNKCFYKHALPNGDIVDVGLPKRTRKLQSQNEIIDLLDIYLWDYVDRRDYHWLEMISSDITSSESSDYSDED

Target ORF information:

RefSeq Version	NM_168922.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster makorin 1, transcript variant B (Mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168922.3

ORF Insert Sequence:

ATGAGTGCCG TCACCAGCAG CGATACAGCC ATCAGCGGCA TGGCTCTTGG CCGGAGCCAG 
ACCATCTGCC GCTACTACGT GCGCGGCATC TGCCGCTTTG GGGAACTTTG CCGCTTCTCC 
CACGACCTGA GCCGCGGTCG TCCCGAGTGC GAGGAGCAGG TGGCCACCGA CGTGCTGCCG 
AAGCCTAGTA CAAGCAGCAG CAGCACCATT GGCAGCCGCA GCGCCAGCAT CTCCAGTCAG 
CAGAGGAACT GGGCGAACGC CCCTGTGTTT GTGCCTAGCC AAAAGCGCTA TACTGCCCAC 
GAACAAAGCG AATTTGAGAC CACTGTGGAC CCTGAGGCGG TGATGGAGGC GCAGGCTGGA 
GCCAGCTACG ATACATTAGC CCCGGGTGTC TCCTGGGCCG AAGTAGTGGG CGGACCTTCG 
TCTCTGAACA AAGAGGACTA TGGCGAGGAG AACAGTTCCT GCGCCTGGGG CGAATTCTCC 
GCTTACCCCA TTCACATGGA GCTGTGCGAA ATGTGCGACC AGTATTGCCT GCATCCCACG 
GATCAAGTTC AGCGGAGGAG TCACAACCGT GAGTGCCTTC AGCAGCACGA GCAGGCCATG 
GAGCTGTCCT TCGCCATTGC CAGATCCAAG GACAAGACGT GCGGCATCTG CTTTGACACG 
ATTATGGAAA AGGCGGGAAG AGAAAAGCGC TTCGGCATTC TCCCCAACTG CAACCACATA 
TTCTGCTTGG AGTGCATTCG CACATGGCGT CAGGCCAAAC AGTTTGAGAA CAAAATAACG 
CGAGCTTGCC CCGAATGTCG CGTGTGCTCG GATTTTGTCT GTCCCAGTGC GTTCTGGATG 
GAGACCAAGG AGGAAAAGGA CAAATTGCTC AACGATTACC GCGCCGCTTT GGGAGCAAAG 
GACTGCAAAT ACTTTAAAAA AGGAGAGGGC AAGTGCCCCT TTGGTAACAA GTGTTTCTAC 
AAGCACGCTC TGCCCAACGG AGATATCGTC GATGTGGGTT TGCCGAAGCG CACCCGAAAG 
TTACAGAGCC AGAATGAAAT AATCGATTTA CTTGATATAT ATCTCTGGGA CTACGTAGAT 
CGGCGAGATT ATCATTGGCT GGAGATGATT TCAAGTGATA TTACTAGTTC CGAAAGTTCA 
GATTACTCGG ATGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mkrn1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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