mkrn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkrn1
Entrez Gene ID	170782
Full Name	makorin, ring finger protein, 1
Synonyms	fl25h08,wu:fl25h08,zgc:110403
General protein information	Preferred Names makorin, ring finger protein, 1 Names probable E3 ubiquitin-protein ligase makorin-1 RING-type E3 ubiquitin transferase makorin-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_152978.1	NP_694510.1	probable E3 ubiquitin-protein ligase makorin-1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MKRN1	NP_990073.1
Drosophila melanogaster (fruit fly)	Mkrn1	NP_001246867.1
Drosophila melanogaster (fruit fly)	CG5347	NP_572970.1
Xenopus tropicalis (tropical clawed frog)	mkrn1	XP_004912513.1
Mus musculus (house mouse)	Mkrn1	NP_061280.2
Homo sapiens (human)	MKRN1	NP_038474.2
Drosophila melanogaster (fruit fly)	CG5334	NP_572969.1
Caenorhabditis elegans (roundworm)	Y55F3AM.6	NP_001023510.1
Pan troglodytes (chimpanzee)	MKRN1	XP_519424.2
	MKRN1	XP_001107668.2
Danio rerio (zebrafish)	mkrn1	NP_694510.1
Canis lupus familiaris (dog)	MKRN1	XP_851764.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
B?hne A, Darras A, D'Cotta H, Baroiller JF, Galiana-Arnoux D, Volff JN
BMC genomics11721(2010 Dec)

Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene.
Gray TA, Azama K, Whitmore K, Min A, Abe S, Nicholls RD
Genomics77(3)119-26(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mkrn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkrn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37505	NM_152978.1 Latest version!	Danio rerio makorin, ring finger protein, 1 (mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkrn1 ( NM_152978.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694510.1 Danio rerio makorin, ring finger protein, 1 (mkrn1), mRNA.

Price & Availability↑

CloneID	ODa37505
Clone ID Related Accession (Same CDS sequence)	NM_152978.1
Accession Version	NM_152978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase makorin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095243.1. ##Evidence-Data-START## Transcript exon combination :: BC095243.1, GDQH01000978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG CAGCGGCAGC GTCAACAGCG GCTCCAGCAG TTATCGGAGG CTGGACGAAG 
CACGTAACCT GCAGGTATTT CATGCACGGG CTTTGCAAAG AGGGTGAGAA CTGTCGATAT 
TCACACGACC TCAGCAGCTG CAAGCAAACC ATGATCTGCA AGTTCTTCCA AAAGGGATGC 
TGTGCTTTCG GGGACCGTTG CAGGTATGAA CATACCAAAC CTTCCAAGCA AGACGAAGTC 
CCCAGTTCGA AGCCGTCGAT GCCGCTGACC GCTGCTCCCC TTGCTGGCAC TCCTGAACCC 
GTGTCTGATG GGCCAGGTGG CACGACCGGC GCCCAGGAGA AGCCCCAAGG CTCAGGGGCA 
GTGGACTGGG TCAATGCCGC CGAGTTCGTG CCAGGGCAGC CCTACTGCGG GAGGGCCGAC 
CCAGTGCTGT GTGAGGGGCC CGGTCCTCTC ATCGAAGAAG AGTACGAGAA AGAACAAGCC 
AACAAAGAAA TGAAGAAGCA GCTTTGCCCA TACGCCGCTG TTGGCGAATG TCGCTATGGC 
CTTAACTGTG CCTACCTCCA CGGCGACGTT TGCGACATGT GCGGCCTACA GGTGCTCCAC 
CCGTCCGACA CCTCTCAGCG CTCGCAACAC ATCAGGGCTT GCATCGAAGC TCACGAGAAG 
GACATGGAAA TCTCGTTTGC CATCCAGCGT AGCAAGGACA TGATGTGCGG CGTGTGTATG 
GAGGTGGTGT TCGAGAAAAC CAACCCTAGC GAACGCCGTT TCGGCATCCT CTCCAACTGC 
TGCCACTGCT ACTGCCTCAA GTGCATCAGG AAATGGCGAA GCGCCAAGCA GTTCGAGAGC 
AAGATCATAA AGTCCTGCCC AGAGTGTCGA ATTACATCAA ACTTTGTCAT ACCAAGCGAA 
TACTGGGTGG AGGACAAGGA GGAAAAGCAG CAACTCATTC AGAAGTACAA GGATGGCATG 
GGAACTAAAC CATGCCGATA TTTTGATGAA GGCCGTGGGA CCTGTCCGTT TGGAGCCAAC 
TGCTTTTACA AGCACGCTTT CCCTGACGGG CGTCTGGAAG AGCCTCAACC CCAGAGGAGA 
CAGAACGGCT CCAATGGAAG AAACCGGAAC ACCAGACGGA CGCATCTCTG GGATCTTTTA 
GACGAGCGGG AGAACAGCGA CTCCTTCGAC AACGAAGACG AGGAGATGGT GAGGTTCGAG 
CTGAGCGAGA TGCTCCTAAT GCTTCTGGCT GCGGGAACCG ACGACGACGT CACCGACTCC 
GAGGACGAGT GGGATTTGTT CCACGAAGAG CTGGACGATT ACTATGAGCT GTACCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694510.1
CDS	14..1333
Translation	MAEAAAASTAAPAVIGGWTKHVTCRYFMHGLCKEGENCRYSHDLSSCKQTMICKFFQKGCCAFGDRCRYEHTKPSKQDEVPSSKPSMPLTAAPLAGTPEPVSDGPGGTTGAQEKPQGSGAVDWVNAAEFVPGQPYCGRADPVLCEGPGPLIEEEYEKEQANKEMKKQLCPYAAVGECRYGLNCAYLHGDVCDMCGLQVLHPSDTSQRSQHIRACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSERRFGILSNCCHCYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEDKEEKQQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDGRLEEPQPQRRQNGSNGRNRNTRRTHLWDLLDERENSDSFDNEDEEMVRFELSEMLLMLLAAGTDDDVTDSEDEWDLFHEELDDYYELYL

Target ORF information:

RefSeq Version	NM_152978.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio makorin, ring finger protein, 1 (mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152978.1

ORF Insert Sequence:

ATGGCGGAGG CAGCGGCAGC GTCAACAGCG GCTCCAGCAG TTATCGGAGG CTGGACGAAG 
CACGTAACCT GCAGGTATTT CATGCACGGG CTTTGCAAAG AGGGTGAGAA CTGTCGATAT 
TCACACGACC TCAGCAGCTG CAAGCAAACC ATGATCTGCA AGTTCTTCCA AAAGGGATGC 
TGTGCTTTCG GGGACCGTTG CAGGTATGAA CATACCAAAC CTTCCAAGCA AGACGAAGTC 
CCCAGTTCGA AGCCGTCGAT GCCGCTGACC GCTGCTCCCC TTGCTGGCAC TCCTGAACCC 
GTGTCTGATG GGCCAGGTGG CACGACCGGC GCCCAGGAGA AGCCCCAAGG CTCAGGGGCA 
GTGGACTGGG TCAATGCCGC CGAGTTCGTG CCAGGGCAGC CCTACTGCGG GAGGGCCGAC 
CCAGTGCTGT GTGAGGGGCC CGGTCCTCTC ATCGAAGAAG AGTACGAGAA AGAACAAGCC 
AACAAAGAAA TGAAGAAGCA GCTTTGCCCA TACGCCGCTG TTGGCGAATG TCGCTATGGC 
CTTAACTGTG CCTACCTCCA CGGCGACGTT TGCGACATGT GCGGCCTACA GGTGCTCCAC 
CCGTCCGACA CCTCTCAGCG CTCGCAACAC ATCAGGGCTT GCATCGAAGC TCACGAGAAG 
GACATGGAAA TCTCGTTTGC CATCCAGCGT AGCAAGGACA TGATGTGCGG CGTGTGTATG 
GAGGTGGTGT TCGAGAAAAC CAACCCTAGC GAACGCCGTT TCGGCATCCT CTCCAACTGC 
TGCCACTGCT ACTGCCTCAA GTGCATCAGG AAATGGCGAA GCGCCAAGCA GTTCGAGAGC 
AAGATCATAA AGTCCTGCCC AGAGTGTCGA ATTACATCAA ACTTTGTCAT ACCAAGCGAA 
TACTGGGTGG AGGACAAGGA GGAAAAGCAG CAACTCATTC AGAAGTACAA GGATGGCATG 
GGAACTAAAC CATGCCGATA TTTTGATGAA GGCCGTGGGA CCTGTCCGTT TGGAGCCAAC 
TGCTTTTACA AGCACGCTTT CCCTGACGGG CGTCTGGAAG AGCCTCAACC CCAGAGGAGA 
CAGAACGGCT CCAATGGAAG AAACCGGAAC ACCAGACGGA CGCATCTCTG GGATCTTTTA 
GACGAGCGGG AGAACAGCGA CTCCTTCGAC AACGAAGACG AGGAGATGGT GAGGTTCGAG 
CTGAGCGAGA TGCTCCTAAT GCTTCTGGCT GCGGGAACCG ACGACGACGT CACCGACTCC 
GAGGACGAGT GGGATTTGTT CCACGAAGAG CTGGACGATT ACTATGAGCT GTACCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
BMC genomics11721(2010 Dec)
B?hne A,Darras A,D'Cotta H,Baroiller JF,Galiana-Arnoux D,Volff JN

Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene.
Genomics77(3)119-26(2001 Oct)
Gray TA,Azama K,Whitmore K,Min A,Abe S,Nicholls RD

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mkrn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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