MKRN1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001291663.1	NP_001278592.1	E3 ubiquitin-protein ligase makorin-1 isoform 3
NM_013446.3	NP_038474.2	E3 ubiquitin-protein ligase makorin-1 isoform 1
XM_011515997.3	XP_011514299.1	E3 ubiquitin-protein ligase makorin-1 isoform X2
XM_011515996.1	XP_011514298.1	E3 ubiquitin-protein ligase makorin-1 isoform X1
XM_011515996.2	XP_011514298.1	E3 ubiquitin-protein ligase makorin-1 isoform X1
XM_011515998.1	XP_011514300.1	E3 ubiquitin-protein ligase makorin-1 isoform X2
NM_001145125.1	NP_001138597.1	E3 ubiquitin-protein ligase makorin-1 isoform 2
NM_013446.4	NP_038474.2	E3 ubiquitin-protein ligase makorin-1 isoform 1
XM_011515997.2	XP_011514299.1	E3 ubiquitin-protein ligase makorin-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17037	NM_013446.4 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17042	NM_001145125.1 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu66359	XM_011515997.3 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu66359	XM_011515998.1 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu24559	NM_001291663.1 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu66358	XM_011515996.2 Latest version!	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu66358	XM_011515996.1	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu17037	NM_013446.3	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu66359	XM_011515997.2	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

MKRN1 ( NM_013446.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_038474.2 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17037
Clone ID Related Accession (Same CDS sequence)	NM_013446.3 , NM_013446.4
Accession Version	NM_013446.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069335.17, BC037400.1 and AI828363.1. On Feb 12, 2009 this sequence version replaced NM_013446.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.89511.1, SRR1660809.61132.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTCC TGCCCAGAAT GCCGGATCAC ATCTAACTTT 
GTCATTCCAA GTGAGTACTG GGTGGAGGAG AAAGAAGAGA AGCAGAAACT CATTCTGAAA 
TACAAGGAGG CAATGAGCAA CAAGGCGTGC AGGTATTTTG ATGAAGGACG TGGGAGCTGC 
CCATTTGGAG GGAACTGTTT TTACAAGCAT GCGTACCCTG ATGGCCGTAG AGAGGAGCCA 
CAGAGACAGA AAGTGGGAAC ATCAAGCAGA TACCGGGCCC AACGAAGGAA CCACTTCTGG 
GAACTCATTG AGGAAAGAGA GAACAGCAAC CCCTTTGACA ACGATGAAGA AGAGGTTGTC 
ACCTTTGAGC TGGGCGAGAT GTTGCTTATG CTTTTGGCTG CAGGTGGGGA CGACGAACTA 
ACAGACTCTG AAGATGAGTG GGACTTGTTT CATGATGAGC TGGAAGATTT TTATGACTTG 
GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038474.2
CDS	226..1674
Translation	MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	NM_013446.3
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013446.3

ORF Insert Sequence:

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTCC TGCCCAGAAT GCCGGATCAC ATCTAACTTT 
GTCATTCCAA GTGAGTACTG GGTGGAGGAG AAAGAAGAGA AGCAGAAACT CATTCTGAAA 
TACAAGGAGG CAATGAGCAA CAAGGCGTGC AGGTATTTTG ATGAAGGACG TGGGAGCTGC 
CCATTTGGAG GGAACTGTTT TTACAAGCAT GCGTACCCTG ATGGCCGTAG AGAGGAGCCA 
CAGAGACAGA AAGTGGGAAC ATCAAGCAGA TACCGGGCCC AACGAAGGAA CCACTTCTGG 
GAACTCATTG AGGAAAGAGA GAACAGCAAC CCCTTTGACA ACGATGAAGA AGAGGTTGTC 
ACCTTTGAGC TGGGCGAGAT GTTGCTTATG CTTTTGGCTG CAGGTGGGGA CGACGAACTA 
ACAGACTCTG AAGATGAGTG GGACTTGTTT CATGATGAGC TGGAAGATTT TTATGACTTG 
GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( NM_013446.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_038474.2 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17037
Clone ID Related Accession (Same CDS sequence)	NM_013446.3 , NM_013446.4
Accession Version	NM_013446.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069335.17, BC037400.1 and AI828363.1. On Nov 22, 2018 this sequence version replaced NM_013446.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.255709.1, BC025955.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255977.7/ ENSP00000255977.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTCC TGCCCAGAAT GCCGGATCAC ATCTAACTTT 
GTCATTCCAA GTGAGTACTG GGTGGAGGAG AAAGAAGAGA AGCAGAAACT CATTCTGAAA 
TACAAGGAGG CAATGAGCAA CAAGGCGTGC AGGTATTTTG ATGAAGGACG TGGGAGCTGC 
CCATTTGGAG GGAACTGTTT TTACAAGCAT GCGTACCCTG ATGGCCGTAG AGAGGAGCCA 
CAGAGACAGA AAGTGGGAAC ATCAAGCAGA TACCGGGCCC AACGAAGGAA CCACTTCTGG 
GAACTCATTG AGGAAAGAGA GAACAGCAAC CCCTTTGACA ACGATGAAGA AGAGGTTGTC 
ACCTTTGAGC TGGGCGAGAT GTTGCTTATG CTTTTGGCTG CAGGTGGGGA CGACGAACTA 
ACAGACTCTG AAGATGAGTG GGACTTGTTT CATGATGAGC TGGAAGATTT TTATGACTTG 
GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038474.2
CDS	162..1610
Translation	MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	NM_013446.4
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013446.4

ORF Insert Sequence:

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTCC TGCCCAGAAT GCCGGATCAC ATCTAACTTT 
GTCATTCCAA GTGAGTACTG GGTGGAGGAG AAAGAAGAGA AGCAGAAACT CATTCTGAAA 
TACAAGGAGG CAATGAGCAA CAAGGCGTGC AGGTATTTTG ATGAAGGACG TGGGAGCTGC 
CCATTTGGAG GGAACTGTTT TTACAAGCAT GCGTACCCTG ATGGCCGTAG AGAGGAGCCA 
CAGAGACAGA AAGTGGGAAC ATCAAGCAGA TACCGGGCCC AACGAAGGAA CCACTTCTGG 
GAACTCATTG AGGAAAGAGA GAACAGCAAC CCCTTTGACA ACGATGAAGA AGAGGTTGTC 
ACCTTTGAGC TGGGCGAGAT GTTGCTTATG CTTTTGGCTG CAGGTGGGGA CGACGAACTA 
ACAGACTCTG AAGATGAGTG GGACTTGTTT CATGATGAGC TGGAAGATTT TTATGACTTG 
GATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( NM_001145125.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138597.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17042
Clone ID Related Accession (Same CDS sequence)	NM_001145125.1
Accession Version	NM_001145125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069335.17, AM236048.1 and CA438497.1. Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site that is used in variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117233.1, SRR1660805.18450.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138597.1
CDS	226..1215
Translation	MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIK

Target ORF information:

RefSeq Version	NM_001145125.1
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145125.1

ORF Insert Sequence:

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGGTATT TTATGCATGG GGTTTGTAAG GAAGGAGACA ACTGTCGCTA CTCGCATGAC 
CTCTCTGACA GTCCGTATAG TGTAGTGTGC AAGTATTTTC AGCGAGGGTA CTGTATTTAT 
GGAGACCGCT GCAGATATGA ACATAGCAAA CCATTGAAAC AGGAAGAAGC AACTGCTACA 
GAGCTAACTA CAAAGTCATC CCTTGCTGCT TCCTCAAGTC TCTCATCGAT AGTTGGACCA 
CTTGTTGAAA TGAATACAGG CGAAGCTGAG TCAAGAAATT CAAACTTTGC AACTGTAGGA 
GCAGGTTCAG AGGACTGGGT GAATGCTATT GAGTTTGTTC CTGGGCAACC CTACTGTGGC 
CGTACTGCGC CTTCCTGCAC TGAAGCACCC CTGCAGGGCT CAGTGACCAA GGAAGAATCA 
GAGAAAGAGC AAACCGCCGT GGAGACAAAG AAGCAGCTGT GCCCCTATGC TGCAGTGGGA 
GAGTGCCGAT ACGGGGAGAA CTGTGTGTAT CTCCACGGAG ATTCTTGTGA CATGTGTGGG 
CTGCAGGTCC TGCATCCAAT GGATGCTGCC CAGAGATCGC AGCATATCAA ATCGTGCATT 
GAGGCCCATG AGAAGGACAT GGAGCTCTCA TTTGCCGTGC AGCGCAGCAA GGACATGGTG 
TGTGGGATCT GCATGGAGGT GGTCTATGAG AAAGCCAACC CCAGTGAGCG CCGCTTCGGG 
ATCCTCTCCA ACTGCAACCA CACCTACTGT CTCAAGTGCA TTCGCAAGTG GAGGAGTGCT 
AAGCAATTTG AGAGCAAGAT CATAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( XM_011515997.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514299.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66359
Clone ID Related Accession (Same CDS sequence)	XM_011515997.3 , XM_011515998.1 , XM_011515997.2
Accession Version	XM_011515997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514299.1
CDS	446..1702
Translation	MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	XM_011515997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515997.3

ORF Insert Sequence:

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( XM_011515998.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514300.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X3, mRNA.

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CloneID	OHu66359
Clone ID Related Accession (Same CDS sequence)	XM_011515997.3 , XM_011515998.1 , XM_011515997.2
Accession Version	XM_011515998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514300.1
CDS	160..1416
Translation	MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	XM_011515998.1
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515998.1

ORF Insert Sequence:

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( XM_011515997.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514299.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.

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CloneID	OHu66359
Clone ID Related Accession (Same CDS sequence)	XM_011515997.3 , XM_011515998.1 , XM_011515997.2
Accession Version	XM_011515997.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514299.1
CDS	475..1731
Translation	MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	XM_011515997.2
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515997.2

ORF Insert Sequence:

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTCCTG CCCAGAATGC CGGATCACAT CTAACTTTGT CATTCCAAGT 
GAGTACTGGG TGGAGGAGAA AGAAGAGAAG CAGAAACTCA TTCTGAAATA CAAGGAGGCA 
ATGAGCAACA AGGCGTGCAG GTATTTTGAT GAAGGACGTG GGAGCTGCCC ATTTGGAGGG 
AACTGTTTTT ACAAGCATGC GTACCCTGAT GGCCGTAGAG AGGAGCCACA GAGACAGAAA 
GTGGGAACAT CAAGCAGATA CCGGGCCCAA CGAAGGAACC ACTTCTGGGA ACTCATTGAG 
GAAAGAGAGA ACAGCAACCC CTTTGACAAC GATGAAGAAG AGGTTGTCAC CTTTGAGCTG 
GGCGAGATGT TGCTTATGCT TTTGGCTGCA GGTGGGGACG ACGAACTAAC AGACTCTGAA 
GATGAGTGGG ACTTGTTTCA TGATGAGCTG GAAGATTTTT ATGACTTGGA TCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( NM_001291663.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278592.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24559
Clone ID Related Accession (Same CDS sequence)	NM_001291663.1
Accession Version	NM_001291663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC400746.1, AC069335.17, BC051821.1 and CA438497.1. Transcript Variant: This variant (3) has multiple differences compared to variant 1. These differences result in distinct 5' and 3' UTRs and cause translation initiation at a downstream start codon compared to variant 1. The 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, and a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051821.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278592.1
CDS	580..1377
Translation	MHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIK

Target ORF information:

RefSeq Version	NM_001291663.1
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291663.1

ORF Insert Sequence:

ATGCATGGGG TTTGTAAGGA AGGAGACAAC TGTCGCTACT CGCATGACCT CTCTGACAGT 
CCGTATAGTG TAGTGTGCAA GTATTTTCAG CGAGGGTACT GTATTTATGG AGACCGCTGC 
AGATATGAAC ATAGCAAACC ATTGAAACAG GAAGAAGCAA CTGCTACAGA GCTAACTACA 
AAGTCATCCC TTGCTGCTTC CTCAAGTCTC TCATCGATAG TTGGACCACT TGTTGAAATG 
AATACAGGCG AAGCTGAGTC AAGAAATTCA AACTTTGCAA CTGTAGGAGC AGGTTCAGAG 
GACTGGGTGA ATGCTATTGA GTTTGTTCCT GGGCAACCCT ACTGTGGCCG TACTGCGCCT 
TCCTGCACTG AAGCACCCCT GCAGGGCTCA GTGACCAAGG AAGAATCAGA GAAAGAGCAA 
ACCGCCGTGG AGACAAAGAA GCAGCTGTGC CCCTATGCTG CAGTGGGAGA GTGCCGATAC 
GGGGAGAACT GTGTGTATCT CCACGGAGAT TCTTGTGACA TGTGTGGGCT GCAGGTCCTG 
CATCCAATGG ATGCTGCCCA GAGATCGCAG CATATCAAAT CGTGCATTGA GGCCCATGAG 
AAGGACATGG AGCTCTCATT TGCCGTGCAG CGCAGCAAGG ACATGGTGTG TGGGATCTGC 
ATGGAGGTGG TCTATGAGAA AGCCAACCCC AGTGAGCGCC GCTTCGGGAT CCTCTCCAAC 
TGCAACCACA CCTACTGTCT CAAGTGCATT CGCAAGTGGA GGAGTGCTAA GCAATTTGAG 
AGCAAGATCA TAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( XM_011515996.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514298.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66358
Clone ID Related Accession (Same CDS sequence)	XM_011515996.1 , XM_011515996.2
Accession Version	XM_011515996.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGATATG AACATAGCAA ACCATTGAAA CAGGAAGAAG CAACTGCTAC AGAGCTAACT 
ACAAAGTCAT CCCTTGCTGC TTCCTCAAGT CTCTCATCGA TAGTTGGACC ACTTGTTGAA 
ATGAATACAG GCGAAGCTGA GTCAAGAAAT TCAAACTTTG CAACTGTAGG AGCAGGTTCA 
GAGGACTGGG TGAATGCTAT TGAGTTTGTT CCTGGGCAAC CCTACTGTGG CCGTACTGCG 
CCTTCCTGCA CTGAAGCACC CCTGCAGGGC TCAGTGACCA AGGAAGAATC AGAGAAAGAG 
CAAACCGCCG TGGAGACAAA GAAGCAGCTG TGCCCCTATG CTGCAGTGGG AGAGTGCCGA 
TACGGGGAGA ACTGTGTGTA TCTCCACGGA GATTCTTGTG ACATGTGTGG GCTGCAGGTC 
CTGCATCCAA TGGATGCTGC CCAGAGATCG CAGCATATCA AATCGTGCAT TGAGGCCCAT 
GAGAAGGACA TGGAGCTCTC ATTTGCCGTG CAGCGCAGCA AGGACATGGT GTGTGGGATC 
TGCATGGAGG TGGTCTATGA GAAAGCCAAC CCCAGTGAGC GCCGCTTCGG GATCCTCTCC 
AACTGCAACC ACACCTACTG TCTCAAGTGC ATTCGCAAGT GGAGGAGTGC TAAGCAATTT 
GAGAGCAAGA TCATAAAGTC CTGCCCAGAA TGCCGGATCA CATCTAACTT TGTCATTCCA 
AGTGAGTACT GGGTGGAGGA GAAAGAAGAG AAGCAGAAAC TCATTCTGAA ATACAAGGAG 
GCAATGAGCA ACAAGGCGTG CAGGTATTTT GATGAAGGAC GTGGGAGCTG CCCATTTGGA 
GGGAACTGTT TTTACAAGCA TGCGTACCCT GATGGCCGTA GAGAGGAGCC ACAGAGACAG 
AAAGTGGGAA CATCAAGCAG ATACCGGGCC CAACGAAGGA ACCACTTCTG GGAACTCATT 
GAGGAAAGAG AGAACAGCAA CCCCTTTGAC AACGATGAAG AAGAGGTTGT CACCTTTGAG 
CTGGGCGAGA TGTTGCTTAT GCTTTTGGCT GCAGGTGGGG ACGACGAACT AACAGACTCT 
GAAGATGAGT GGGACTTGTT TCATGATGAG CTGGAAGATT TTTATGACTT GGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514298.1
CDS	144..1463
Translation	MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	XM_011515996.1
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515996.1

ORF Insert Sequence:

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGATATG AACATAGCAA ACCATTGAAA CAGGAAGAAG CAACTGCTAC AGAGCTAACT 
ACAAAGTCAT CCCTTGCTGC TTCCTCAAGT CTCTCATCGA TAGTTGGACC ACTTGTTGAA 
ATGAATACAG GCGAAGCTGA GTCAAGAAAT TCAAACTTTG CAACTGTAGG AGCAGGTTCA 
GAGGACTGGG TGAATGCTAT TGAGTTTGTT CCTGGGCAAC CCTACTGTGG CCGTACTGCG 
CCTTCCTGCA CTGAAGCACC CCTGCAGGGC TCAGTGACCA AGGAAGAATC AGAGAAAGAG 
CAAACCGCCG TGGAGACAAA GAAGCAGCTG TGCCCCTATG CTGCAGTGGG AGAGTGCCGA 
TACGGGGAGA ACTGTGTGTA TCTCCACGGA GATTCTTGTG ACATGTGTGG GCTGCAGGTC 
CTGCATCCAA TGGATGCTGC CCAGAGATCG CAGCATATCA AATCGTGCAT TGAGGCCCAT 
GAGAAGGACA TGGAGCTCTC ATTTGCCGTG CAGCGCAGCA AGGACATGGT GTGTGGGATC 
TGCATGGAGG TGGTCTATGA GAAAGCCAAC CCCAGTGAGC GCCGCTTCGG GATCCTCTCC 
AACTGCAACC ACACCTACTG TCTCAAGTGC ATTCGCAAGT GGAGGAGTGC TAAGCAATTT 
GAGAGCAAGA TCATAAAGTC CTGCCCAGAA TGCCGGATCA CATCTAACTT TGTCATTCCA 
AGTGAGTACT GGGTGGAGGA GAAAGAAGAG AAGCAGAAAC TCATTCTGAA ATACAAGGAG 
GCAATGAGCA ACAAGGCGTG CAGGTATTTT GATGAAGGAC GTGGGAGCTG CCCATTTGGA 
GGGAACTGTT TTTACAAGCA TGCGTACCCT GATGGCCGTA GAGAGGAGCC ACAGAGACAG 
AAAGTGGGAA CATCAAGCAG ATACCGGGCC CAACGAAGGA ACCACTTCTG GGAACTCATT 
GAGGAAAGAG AGAACAGCAA CCCCTTTGAC AACGATGAAG AAGAGGTTGT CACCTTTGAG 
CTGGGCGAGA TGTTGCTTAT GCTTTTGGCT GCAGGTGGGG ACGACGAACT AACAGACTCT 
GAAGATGAGT GGGACTTGTT TCATGATGAG CTGGAAGATT TTTATGACTT GGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN1 ( XM_011515996.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514298.1 Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66358
Clone ID Related Accession (Same CDS sequence)	XM_011515996.1 , XM_011515996.2
Accession Version	XM_011515996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase makorin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGATATG AACATAGCAA ACCATTGAAA CAGGAAGAAG CAACTGCTAC AGAGCTAACT 
ACAAAGTCAT CCCTTGCTGC TTCCTCAAGT CTCTCATCGA TAGTTGGACC ACTTGTTGAA 
ATGAATACAG GCGAAGCTGA GTCAAGAAAT TCAAACTTTG CAACTGTAGG AGCAGGTTCA 
GAGGACTGGG TGAATGCTAT TGAGTTTGTT CCTGGGCAAC CCTACTGTGG CCGTACTGCG 
CCTTCCTGCA CTGAAGCACC CCTGCAGGGC TCAGTGACCA AGGAAGAATC AGAGAAAGAG 
CAAACCGCCG TGGAGACAAA GAAGCAGCTG TGCCCCTATG CTGCAGTGGG AGAGTGCCGA 
TACGGGGAGA ACTGTGTGTA TCTCCACGGA GATTCTTGTG ACATGTGTGG GCTGCAGGTC 
CTGCATCCAA TGGATGCTGC CCAGAGATCG CAGCATATCA AATCGTGCAT TGAGGCCCAT 
GAGAAGGACA TGGAGCTCTC ATTTGCCGTG CAGCGCAGCA AGGACATGGT GTGTGGGATC 
TGCATGGAGG TGGTCTATGA GAAAGCCAAC CCCAGTGAGC GCCGCTTCGG GATCCTCTCC 
AACTGCAACC ACACCTACTG TCTCAAGTGC ATTCGCAAGT GGAGGAGTGC TAAGCAATTT 
GAGAGCAAGA TCATAAAGTC CTGCCCAGAA TGCCGGATCA CATCTAACTT TGTCATTCCA 
AGTGAGTACT GGGTGGAGGA GAAAGAAGAG AAGCAGAAAC TCATTCTGAA ATACAAGGAG 
GCAATGAGCA ACAAGGCGTG CAGGTATTTT GATGAAGGAC GTGGGAGCTG CCCATTTGGA 
GGGAACTGTT TTTACAAGCA TGCGTACCCT GATGGCCGTA GAGAGGAGCC ACAGAGACAG 
AAAGTGGGAA CATCAAGCAG ATACCGGGCC CAACGAAGGA ACCACTTCTG GGAACTCATT 
GAGGAAAGAG AGAACAGCAA CCCCTTTGAC AACGATGAAG AAGAGGTTGT CACCTTTGAG 
CTGGGCGAGA TGTTGCTTAT GCTTTTGGCT GCAGGTGGGG ACGACGAACT AACAGACTCT 
GAAGATGAGT GGGACTTGTT TCATGATGAG CTGGAAGATT TTTATGACTT GGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514298.1
CDS	24..1343
Translation	MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Target ORF information:

RefSeq Version	XM_011515996.2
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515996.2

ORF Insert Sequence:

ATGGCGGAGG CTGCAACTCC CGGAACAACA GCCACAACAT CAGGAGCAGG AGCGGCAGCG 
GCGACGGCGG CAGCAGCCTC CCCCACCCCG ATCCCCACAG TCACCGCCCC GTCCCTGGGG 
GCGGGCGGAG GGGGCGGCGG CAGCGACGGC AGCGGCGGCG GCTGGACTAA ACAGGTCACC 
TGCAGATATG AACATAGCAA ACCATTGAAA CAGGAAGAAG CAACTGCTAC AGAGCTAACT 
ACAAAGTCAT CCCTTGCTGC TTCCTCAAGT CTCTCATCGA TAGTTGGACC ACTTGTTGAA 
ATGAATACAG GCGAAGCTGA GTCAAGAAAT TCAAACTTTG CAACTGTAGG AGCAGGTTCA 
GAGGACTGGG TGAATGCTAT TGAGTTTGTT CCTGGGCAAC CCTACTGTGG CCGTACTGCG 
CCTTCCTGCA CTGAAGCACC CCTGCAGGGC TCAGTGACCA AGGAAGAATC AGAGAAAGAG 
CAAACCGCCG TGGAGACAAA GAAGCAGCTG TGCCCCTATG CTGCAGTGGG AGAGTGCCGA 
TACGGGGAGA ACTGTGTGTA TCTCCACGGA GATTCTTGTG ACATGTGTGG GCTGCAGGTC 
CTGCATCCAA TGGATGCTGC CCAGAGATCG CAGCATATCA AATCGTGCAT TGAGGCCCAT 
GAGAAGGACA TGGAGCTCTC ATTTGCCGTG CAGCGCAGCA AGGACATGGT GTGTGGGATC 
TGCATGGAGG TGGTCTATGA GAAAGCCAAC CCCAGTGAGC GCCGCTTCGG GATCCTCTCC 
AACTGCAACC ACACCTACTG TCTCAAGTGC ATTCGCAAGT GGAGGAGTGC TAAGCAATTT 
GAGAGCAAGA TCATAAAGTC CTGCCCAGAA TGCCGGATCA CATCTAACTT TGTCATTCCA 
AGTGAGTACT GGGTGGAGGA GAAAGAAGAG AAGCAGAAAC TCATTCTGAA ATACAAGGAG 
GCAATGAGCA ACAAGGCGTG CAGGTATTTT GATGAAGGAC GTGGGAGCTG CCCATTTGGA 
GGGAACTGTT TTTACAAGCA TGCGTACCCT GATGGCCGTA GAGAGGAGCC ACAGAGACAG 
AAAGTGGGAA CATCAAGCAG ATACCGGGCC CAACGAAGGA ACCACTTCTG GGAACTCATT 
GAGGAAAGAG AGAACAGCAA CCCCTTTGAC AACGATGAAG AAGAGGTTGT CACCTTTGAG 
CTGGGCGAGA TGTTGCTTAT GCTTTTGGCT GCAGGTGGGG ACGACGAACT AACAGACTCT 
GAAGATGAGT GGGACTTGTT TCATGATGAG CTGGAAGATT TTTATGACTT GGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MKRN1
Entrez Gene ID	23608
Full Name	makorin ring finger protein 1
Synonyms	RNF61
General protein information	Preferred Names makorin ring finger protein 1 Names E3 ubiquitin-protein ligase makorin-1 RING finger protein 61 RING-type E3 ubiquitin transferase makorin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q34
Summary	This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014].
Disorder MIM:	607754

Gallus gallus (chicken)	MKRN1	NP_990073.1
Drosophila melanogaster (fruit fly)	Mkrn1	NP_001246867.1
Drosophila melanogaster (fruit fly)	CG5347	NP_572970.1
Xenopus tropicalis (tropical clawed frog)	mkrn1	XP_004912513.1
Mus musculus (house mouse)	Mkrn1	NP_061280.2
Homo sapiens (human)	MKRN1	NP_038474.2
Drosophila melanogaster (fruit fly)	CG5334	NP_572969.1
Caenorhabditis elegans (roundworm)	Y55F3AM.6	NP_001023510.1
Pan troglodytes (chimpanzee)	MKRN1	XP_519424.2
	MKRN1	XP_001107668.2
Danio rerio (zebrafish)	mkrn1	NP_694510.1
Canis lupus familiaris (dog)	MKRN1	XP_851764.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MKRN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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