Ret cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ret
Entrez Gene ID	43875
Full Name	Ret oncogene
Synonyms	CG1061,CG14396,D-ret,DRET,DRet,DmHD-59,DmRet,Dmel\CG14396,Dret,HD-59,MEN2,RET,Reto,dRET,dRet,dret,ret
General protein information	Preferred Names Ret oncogene Names CG14396 gene product from transcript CG14396-RA CG14396-PA CG14396-PB CG14396-PC Ret-PA Ret-PB Ret-PC dret
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39B1-39B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165358.2	NP_724315.1	Ret oncogene, isoform B
NM_057696.4	NP_477044.1	Ret oncogene, isoform A
NM_165359.2	NP_724316.1	Ret oncogene, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ret	NP_477045.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008899	XP_319646.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanistically, Ret is engaged in a positive feedback loop with Wnt/Wingless signalling, modulated by Src and Fak kinases.
Title: Ret receptor tyrosine kinase sustains proliferation and tissue maturation in intestinal epithelia.

We demonstrate that this phospho-S909 arises from an intrinsic RET dual-specificity kinase activity and show that an equivalent serine is required for RET signaling in Drosophila. Our findings reveal dual-specificity and allosteric components for the mechanism of RET activation and signaling with direct implications for drug discovery
Title: RET Functions as a Dual-Specificity Kinase that Requires Allosteric Inputs from Juxtamembrane Elements.

Ret as a regulator in dendrite patterning and adhesion distinct from its role in axon guidance, is proposed.
Title: The Ret receptor regulates sensory neuron dendrite growth and integrin mediated adhesion.

A signaling active version of Ret (Ret(MENB) rescues muscle degeneration, disintegration of mitochondria and ATP content of Pink1 mutants.
Title: Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants.

strong propensity to self-association in the RET-transmembrane underlies - and may be required for - dimer formation and oncogenic activation of juxtamembrane cysteine mutants of RET
Title: Self-association of the transmembrane domain of RET underlies oncogenic activation by MEN2A mutations.

The following Ret gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ret cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15175	NM_165359.2 Latest version!	Drosophila melanogaster Ret oncogene (Ret), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15175	NM_057696.4 Latest version!	Drosophila melanogaster Ret oncogene (Ret), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15175	NM_165358.2 Latest version!	Drosophila melanogaster Ret oncogene (Ret), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ret ( NM_165358.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724315.1 Drosophila melanogaster Ret oncogene (Ret), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15175
Clone ID Related Accession (Same CDS sequence)	NM_165358.2 , NM_057696.4 , NM_165359.2
Accession Version	NM_165358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ret oncogene, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165358.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724315.1
CDS	337..4044
Translation	MESTTIVFVTLLTIITQRKHCAAVDVYFPTTSVKFNMPINEESESIFSKIPLAQFQVLRMEDNRLASDYLYSLEQNPLLRINSSSGEIYMRTDYRSPNSSATFLVTAFPRDQPDHELLNVSHLSLEVTPQPLEEYCSELEHICFWSSAQYTIAESHGPYRRKDFFEPVLIGALNSRAAKYLCPHVSLEYSLNAGSSHFVLKQNRLYTRQTLDHDELNGLNAKAGQLQARITCTVKLSSRDQRKFSRILDIKLLDRNDNGPKLQESSSKFDFYLEQPYFQADEEAGKKVIYVDKDTLEANAHLVYAVHNDSHGLFRPDCHAYEADHTGRPHTIVSCQLRFSRNGVFRETPYCVSLEARDLTIVSRVDAMSATANVCYHINLSKLHESEQELPQALPLRARQHRIFESEEFNGDSAGRSLSPPTVDYDKDVSVYRSAASNFRVVQPDSFLDLMRLRSIRFDIVEDKLGAFGITSTSGIVFVKNPQVLEEAPETIYFLNVTWIDQQRLSHVRVINVHLVHGRPENTSCELKVKSRSQTCAQIKYQSQCVRYCGLATGGGSCQWRGSNSAMFGTRYGSCVPESRYCPDHVCDPLEELNPMACPQDCTPAGRIVGPHSSNENKRGIYSASGTCICEDNGKCSCAPLDEEPKMKKPRKRKNETEAEPLLGVRRGTPPNQPLQDPMLLGVLNVAGFECDRSCMFFVITCPLLFVLLLLCLLIAQRKMLQRRLGKQSMTTSSKQALPESGGGDFALMPLQSGFRFESGDAKWEFPREKLQLDTVLGEGEFGQVLKGFATEIAGLPGITTVAVKMLKKGSNSVEYMALLSEFQLLQEVSHPNVIKLLGACTSSEAPLLIIEYARYGSLRSYLRLSRKIECAGVDFADGVEPVNVKMVLTFAWQICKGMAYLSELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRDRVPVKWMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNLWSLLKTGYRMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLASEFEKLLGNNAKYIDLETNAVSNPLYCGDDSALITTELGEPESLQHLWSPPKIAYDIHDQATSYDQSEEEMPVTSTAPPGYDLPRPLLDATANGQVLRYENDLRFPLNIRKSSCTPSYSNMTSEPPATTSLPHYSVPVKRGRSYLDMTNKSLIPDNLDSREFEKHLSKTISFRFSSLLNLSETKEVSPGWQAEDAV

Target ORF information:

RefSeq Version	NM_165358.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ret oncogene (Ret), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165358.2

ORF Insert Sequence:

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ret ( NM_057696.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477044.1 Drosophila melanogaster Ret oncogene (Ret), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15175
Clone ID Related Accession (Same CDS sequence)	NM_165358.2 , NM_057696.4 , NM_165359.2
Accession Version	NM_057696.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ret oncogene, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057696.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477044.1
CDS	257..3964
Translation	MESTTIVFVTLLTIITQRKHCAAVDVYFPTTSVKFNMPINEESESIFSKIPLAQFQVLRMEDNRLASDYLYSLEQNPLLRINSSSGEIYMRTDYRSPNSSATFLVTAFPRDQPDHELLNVSHLSLEVTPQPLEEYCSELEHICFWSSAQYTIAESHGPYRRKDFFEPVLIGALNSRAAKYLCPHVSLEYSLNAGSSHFVLKQNRLYTRQTLDHDELNGLNAKAGQLQARITCTVKLSSRDQRKFSRILDIKLLDRNDNGPKLQESSSKFDFYLEQPYFQADEEAGKKVIYVDKDTLEANAHLVYAVHNDSHGLFRPDCHAYEADHTGRPHTIVSCQLRFSRNGVFRETPYCVSLEARDLTIVSRVDAMSATANVCYHINLSKLHESEQELPQALPLRARQHRIFESEEFNGDSAGRSLSPPTVDYDKDVSVYRSAASNFRVVQPDSFLDLMRLRSIRFDIVEDKLGAFGITSTSGIVFVKNPQVLEEAPETIYFLNVTWIDQQRLSHVRVINVHLVHGRPENTSCELKVKSRSQTCAQIKYQSQCVRYCGLATGGGSCQWRGSNSAMFGTRYGSCVPESRYCPDHVCDPLEELNPMACPQDCTPAGRIVGPHSSNENKRGIYSASGTCICEDNGKCSCAPLDEEPKMKKPRKRKNETEAEPLLGVRRGTPPNQPLQDPMLLGVLNVAGFECDRSCMFFVITCPLLFVLLLLCLLIAQRKMLQRRLGKQSMTTSSKQALPESGGGDFALMPLQSGFRFESGDAKWEFPREKLQLDTVLGEGEFGQVLKGFATEIAGLPGITTVAVKMLKKGSNSVEYMALLSEFQLLQEVSHPNVIKLLGACTSSEAPLLIIEYARYGSLRSYLRLSRKIECAGVDFADGVEPVNVKMVLTFAWQICKGMAYLSELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRDRVPVKWMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNLWSLLKTGYRMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLASEFEKLLGNNAKYIDLETNAVSNPLYCGDDSALITTELGEPESLQHLWSPPKIAYDIHDQATSYDQSEEEMPVTSTAPPGYDLPRPLLDATANGQVLRYENDLRFPLNIRKSSCTPSYSNMTSEPPATTSLPHYSVPVKRGRSYLDMTNKSLIPDNLDSREFEKHLSKTISFRFSSLLNLSETKEVSPGWQAEDAV

Target ORF information:

RefSeq Version	NM_057696.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ret oncogene (Ret), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057696.4

ORF Insert Sequence:

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ret ( NM_165359.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724316.1 Drosophila melanogaster Ret oncogene (Ret), transcript variant C, mRNA.

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CloneID	OFa15175
Clone ID Related Accession (Same CDS sequence)	NM_165358.2 , NM_057696.4 , NM_165359.2
Accession Version	NM_165359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ret oncogene, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165359.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724316.1
CDS	478..4185
Translation	MESTTIVFVTLLTIITQRKHCAAVDVYFPTTSVKFNMPINEESESIFSKIPLAQFQVLRMEDNRLASDYLYSLEQNPLLRINSSSGEIYMRTDYRSPNSSATFLVTAFPRDQPDHELLNVSHLSLEVTPQPLEEYCSELEHICFWSSAQYTIAESHGPYRRKDFFEPVLIGALNSRAAKYLCPHVSLEYSLNAGSSHFVLKQNRLYTRQTLDHDELNGLNAKAGQLQARITCTVKLSSRDQRKFSRILDIKLLDRNDNGPKLQESSSKFDFYLEQPYFQADEEAGKKVIYVDKDTLEANAHLVYAVHNDSHGLFRPDCHAYEADHTGRPHTIVSCQLRFSRNGVFRETPYCVSLEARDLTIVSRVDAMSATANVCYHINLSKLHESEQELPQALPLRARQHRIFESEEFNGDSAGRSLSPPTVDYDKDVSVYRSAASNFRVVQPDSFLDLMRLRSIRFDIVEDKLGAFGITSTSGIVFVKNPQVLEEAPETIYFLNVTWIDQQRLSHVRVINVHLVHGRPENTSCELKVKSRSQTCAQIKYQSQCVRYCGLATGGGSCQWRGSNSAMFGTRYGSCVPESRYCPDHVCDPLEELNPMACPQDCTPAGRIVGPHSSNENKRGIYSASGTCICEDNGKCSCAPLDEEPKMKKPRKRKNETEAEPLLGVRRGTPPNQPLQDPMLLGVLNVAGFECDRSCMFFVITCPLLFVLLLLCLLIAQRKMLQRRLGKQSMTTSSKQALPESGGGDFALMPLQSGFRFESGDAKWEFPREKLQLDTVLGEGEFGQVLKGFATEIAGLPGITTVAVKMLKKGSNSVEYMALLSEFQLLQEVSHPNVIKLLGACTSSEAPLLIIEYARYGSLRSYLRLSRKIECAGVDFADGVEPVNVKMVLTFAWQICKGMAYLSELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRDRVPVKWMAPESLADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNLWSLLKTGYRMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLASEFEKLLGNNAKYIDLETNAVSNPLYCGDDSALITTELGEPESLQHLWSPPKIAYDIHDQATSYDQSEEEMPVTSTAPPGYDLPRPLLDATANGQVLRYENDLRFPLNIRKSSCTPSYSNMTSEPPATTSLPHYSVPVKRGRSYLDMTNKSLIPDNLDSREFEKHLSKTISFRFSSLLNLSETKEVSPGWQAEDAV

Target ORF information:

RefSeq Version	NM_165359.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ret oncogene (Ret), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165359.2

ORF Insert Sequence:

ATGGAGTCAA CTACTATTGT TTTTGTGACT CTGCTCACAA TTATAACCCA ACGTAAACAC 
TGTGCGGCCG TCGATGTTTA CTTTCCCACC ACGTCGGTGA AATTCAATAT GCCCATCAAT 
GAGGAATCGG AGAGCATATT CTCCAAAATC CCGCTAGCCC AGTTCCAAGT GCTGCGGATG 
GAGGACAACC GGTTAGCCAG TGATTACTTG TATAGCCTGG AGCAGAATCC ACTACTCCGA 
ATAAACAGTT CCTCCGGCGA GATATATATG CGCACTGACT ACCGCTCACC AAACTCAAGT 
GCCACATTCT TGGTGACCGC ATTTCCCAGA GATCAACCGG ATCACGAGCT GCTGAATGTT 
TCGCATCTTT CGTTGGAGGT TACACCTCAG CCCCTGGAGG AGTACTGTTC GGAACTGGAG 
CACATTTGCT TCTGGAGCAG TGCTCAGTAC ACTATAGCAG AGTCGCACGG TCCATATCGG 
CGGAAGGATT TTTTTGAACC CGTACTTATC GGCGCCCTTA ATTCCCGCGC TGCGAAGTAT 
CTGTGTCCTC ATGTATCCCT GGAATATTCC CTGAACGCTG GTAGTTCCCA TTTTGTTTTG 
AAACAAAATC GACTCTACAC CCGACAAACC TTGGATCACG ACGAGCTCAA TGGACTGAAT 
GCCAAGGCAG GGCAGCTGCA GGCCAGGATT ACCTGCACGG TTAAATTGTC CAGCAGGGAT 
CAGAGAAAAT TCTCGCGCAT CTTGGATATC AAGTTACTGG ATCGCAATGA TAATGGACCC 
AAGTTGCAGG AGAGTAGCTC TAAGTTTGAT TTCTATCTGG AGCAGCCCTA CTTCCAAGCG 
GACGAGGAGG CGGGAAAAAA AGTAATCTAC GTGGACAAGG ATACATTGGA GGCAAATGCT 
CACCTTGTCT ACGCCGTCCA CAATGACTCT CATGGTCTGT TTCGGCCCGA CTGCCACGCC 
TACGAGGCGG ATCACACGGG CAGACCACAT ACCATCGTCA GTTGTCAACT GCGATTCTCC 
CGAAACGGTG TCTTCCGGGA AACCCCCTAT TGTGTGTCCT TGGAGGCTCG GGATCTGACC 
ATTGTAAGCC GTGTCGATGC CATGTCAGCG ACAGCCAATG TTTGCTATCA TATTAATCTG 
AGTAAGCTTC ACGAATCTGA GCAAGAATTA CCGCAAGCTC TTCCCCTACG GGCACGTCAA 
CATCGAATAT TCGAGAGCGA AGAATTCAAT GGAGATTCTG CAGGCCGATC TCTAAGTCCT 
CCGACCGTGG ATTACGATAA GGATGTTTCC GTATACAGAT CGGCTGCTTC TAATTTTCGA 
GTTGTCCAGC CTGACAGTTT TTTGGACTTG ATGCGGTTAC GATCTATTCG ATTCGATATT 
GTGGAGGATA AACTTGGAGC TTTTGGTATT ACCTCAACAT CGGGTATTGT CTTTGTGAAG 
AACCCACAGG TTTTGGAGGA GGCACCGGAA ACCATATACT TCCTGAATGT CACCTGGATC 
GATCAGCAAA GGCTGTCGCA CGTGAGAGTG ATCAATGTGC ACTTGGTTCA TGGTAGACCC 
GAGAATACTA GTTGCGAACT GAAGGTCAAG TCTCGATCAC AGACATGTGC CCAGATTAAA 
TACCAATCGC AATGCGTTCG ATATTGCGGC TTGGCCACAG GTGGTGGATC TTGCCAGTGG 
AGGGGGTCCA ACTCAGCCAT GTTCGGCACT AGATATGGTT CCTGTGTACC CGAATCTCGT 
TACTGTCCAG ATCATGTCTG TGATCCCCTA GAGGAACTGA ATCCTATGGC CTGTCCGCAG 
GATTGCACGC CAGCTGGAAG AATCGTGGGT CCCCATTCAA GTAATGAGAA TAAGAGAGGG 
ATATACAGTG CCTCGGGTAC CTGCATTTGC GAGGATAATG GCAAGTGCTC GTGCGCTCCG 
TTAGATGAGG AACCCAAGAT GAAGAAACCG CGAAAACGAA AAAACGAAAC AGAGGCGGAA 
CCTCTGCTGG GGGTACGAAG GGGCACTCCT CCGAATCAGC CACTTCAGGA TCCCATGCTT 
CTGGGTGTCC TAAATGTGGC CGGTTTCGAA TGCGATCGCT CCTGCATGTT CTTCGTGATC 
ACGTGCCCTC TATTGTTCGT TCTCCTGCTC CTCTGTTTGC TGATTGCGCA GAGAAAGATG 
CTCCAACGTC GCTTGGGCAA GCAATCAATG ACCACGTCGT CGAAACAAGC TCTTCCGGAA 
TCAGGAGGCG GAGATTTCGC CTTGATGCCG CTGCAGAGTG GCTTCAGGTT CGAAAGTGGG 
GATGCCAAAT GGGAGTTTCC CAGGGAAAAA CTGCAACTAG ATACGGTCTT GGGAGAGGGT 
GAATTTGGTC AGGTGCTAAA GGGCTTTGCC ACCGAGATCG CTGGCTTGCC GGGAATAACC 
ACGGTGGCCG TAAAGATGCT CAAGAAGGGC TCCAATTCAG TGGAGTACAT GGCCCTGCTT 
TCGGAGTTTC AGCTCCTCCA GGAGGTCTCT CACCCGAATG TGATCAAGCT GCTAGGCGCC 
TGCACCTCCT CCGAAGCACC TCTCCTGATC ATCGAGTATG CTCGGTATGG CTCTCTGAGG 
AGCTATCTTC GACTCAGTCG GAAGATCGAG TGTGCCGGCG TAGATTTCGC AGATGGAGTG 
GAGCCTGTTA ATGTTAAGAT GGTACTTACC TTTGCTTGGC AGATTTGCAA GGGTATGGCT 
TACCTTTCTG AGTTAAAGTT GGTTCATCGT GATTTGGCTG CTAGAAATGT GCTCCTTGCG 
GATGGCAAGA TATGCAAAAT ATCAGATTTC GGACTGACTC GAGATGTTTA CGAGGACGAT 
GCCTATTTAA AGAGATCCCG AGATCGTGTG CCCGTCAAGT GGATGGCTCC GGAATCTTTA 
GCGGATCATG TGTATACCAG CAAATCGGAT GTGTGGTCCT TTGGCGTTCT CTGCTGGGAA 
CTAATCACTC TGGGAGCCTC TCCGTATCCT GGCATTGCTC CCCAGAATTT GTGGTCCTTG 
CTGAAGACGG GCTACCGCAT GGACAGACCA GAAAACTGTT CGGAGGCTGT CTACTCTATA 
GTTCGAACTT GCTGGGCAGA CGAGCCAAAT GGAAGACCCT CATTCAAGTT TTTAGCATCG 
GAGTTTGAGA AGCTATTGGG AAACAATGCC AAGTACATAG ATCTGGAAAC GAATGCCGTT 
TCGAATCCCC TTTATTGTGG GGATGATTCC GCCTTAATAA CCACGGAATT GGGCGAACCA 
GAATCGTTGC AGCACCTTTG GTCACCTCCC AAAATAGCCT ACGACATCCA TGACCAGGCC 
ACCAGCTACG ATCAGTCTGA GGAGGAGATG CCAGTGACTT CAACGGCTCC GCCGGGTTAC 
GACTTACCAC GACCTTTGCT TGATGCTACC GCCAACGGGC AGGTTTTGCG ATACGAAAAC 
GATTTGCGAT TCCCCTTAAA TATTCGGAAA TCCAGTTGTA CTCCAAGCTA CAGCAACATG 
ACCAGTGAAC CTCCAGCGAC CACCTCACTG CCACATTATT CTGTCCCCGT GAAGAGGGGT 
CGATCCTACC TGGATATGAC CAACAAGAGT CTCATCCCAG ACAACCTGGA CAGCAGGGAG 
TTTGAAAAGC ATCTGTCCAA GACCATCTCG TTCCGTTTCT CTAGTTTGCT GAATCTCAGT 
GAAACGAAGG AGGTGAGTCC AGGATGGCAA GCTGAGGATG CAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ret cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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