Gfat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfat2
Entrez Gene ID	43373
Full Name	Glutamine:fructose-6-phosphate aminotransferase 2
Synonyms	CG1345,Dmel/GFAT2,Dmel\CG1345,gfat2
General protein information	Preferred Names Glutamine:fructose-6-phosphate aminotransferase 2 Names CG1345 gene product from transcript CG1345-RA CG1345-PA Gfat2-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98C4-98C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_143360.4	NP_651617.1	Glutamine:fructose-6-phosphate aminotransferase 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GFPT2	XP_003642114.1
Drosophila melanogaster (fruit fly)	Gfat1	NP_001015157.2
Saccharomyces cerevisiae (baker's yeast)	GFA1	NP_012818.1
Eremothecium gossypii	AGOS_ABL036C	NP_982911.2
Oryza sativa (rice)	Os12g0131100	NP_001066074.1
	GFPT2	XP_001106226.2
Canis lupus familiaris (dog)	GFPT2	XP_005626343.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011352	XP_309298.2
Neurospora crassa	NCU11350	XP_957994.2
Pan troglodytes (chimpanzee)	GFPT2	XP_518158.4
Rattus norvegicus (Norway rat)	Gfpt2	NP_001002819.2
Caenorhabditis elegans (roundworm)	F22B3.4	NP_502156.1
Arabidopsis thaliana (thale cress)	AT3G24090	NP_189051.3
Drosophila melanogaster (fruit fly)	Gfat2	NP_651617.1
Caenorhabditis elegans (roundworm)	F07A11.2	NP_496479.1
Kluyveromyces lactis	KLLA0F13596g	XP_455692.1
Homo sapiens (human)	GFPT2	NP_005101.1
Bos taurus (cattle)	GFPT2	NP_001070351.1
Mus musculus (house mouse)	Gfpt2	NP_038557.1
Danio rerio (zebrafish)	gfpt2	NP_001029093.1
Schizosaccharomyces pombe (fission yeast)	SPBC12C2.11	NP_596011.1
Magnaporthe oryzae (rice blast fungus)	MGG_11597	XP_003718755.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gfat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06388	NM_143360.4 Latest version!	Drosophila melanogaster Glutamine:fructose-6-phosphate aminotransferase 2 (Gfat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfat2 ( NM_143360.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651617.1 Drosophila melanogaster Glutamine:fructose-6-phosphate aminotransferase 2 (Gfat2), mRNA.

Price & Availability↑

CloneID	OFa06388
Clone ID Related Accession (Same CDS sequence)	NM_143360.4
Accession Version	NM_143360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamine:fructose-6-phosphate aminotransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143360.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGGCA TATTCGCGTA CCTAAACTAC TTGACGCCCA AGTCGCGACA GGAGGTCCTG 
GATCTCCTGT TGCAGGGACT GAAGCGGCTG GAGTACCGCG GCTACGACTC CACGGGCATA 
GCCATCGATG CGCTGAACTC TGGCGAGGCA CAGTCCATAA TGCTGGTGAA GCGCACCGGG 
AAGGTGAAGG TGCTGGAGGA TGCGGTGGCG GAGGTGTGTC GCGGCCAGGA CTACTCGCTG 
CCCATCGACA CCCACATAGG CATAGCACAC ACGCGCTGGG CCACCCACGG TGTGCCCTCC 
GAGGTCAACT CGCATCCGCA GCGATCCGAT GAGGATAATA GCTTTGTGGT GGTGCACAAT 
GGCATTATAA CCAACTACAA GGATGTGAAG ACGCTGCTGG AGAAGCGCGG ATATGTATTT 
GAGTCCGACA CGGACACGGA GGTGATTGCC AAGCTGGTAC ACCACCTGTG GCAGCAGCAT 
CCTGGCTACA CTTTCGGCGA GCTCGTGGAG CAGGCCATTC AGCAGCTGGA GGGCGCCTTC 
GCCATCGCCT TCAAGTCGAA GCACTTCCCG GGCGAGTGCG TGGCCTCCAG GCGAGGCTCA 
CCGCTTCTGG TGGGCATCAA GGCCAAGACC AAGCTGGCCA CCGATCACAT ACCCATCCTG 
TACGCCAAGG CCCATCGGCC ACATGGTCAG CCGCAGCAGC AGGCCTATCA ACTATTGCCG 
CCCGGCGATT GCAACGCCGA GTTTCAACCG CTGGAGCGCA AGGAAGTGGA GTACTTCTTC 
GCCTCGGACG CATCGGCTGT TATAGAGCAC ACGAATCGGG TGATTTACCT GGAAGATGAC 
GACGTGGCCG CCGTCAAGAG CGATGGCACG CTCAGCATTC ATCGCCTAAA CAAATCATCC 
GATGATCCGC ACGCCCGCGA GATCATCACG CTGAAAATGG AGATCCAGCA GATCATGAAG 
GGCAACTACG ACTACTTTAT GCTGAAGGAG ATCTTCGAGC AGCCGGAATC CGTGGTCAAC 
ACCATGCGCG GCAGGATGAG GTTCGATACG CAGACCGTGG TTCTGGGTGG CATCAAGGAG 
TACATACCGG AGATCAAGCG CTGCCGCCGC TTGATGCTAA TTGCGTGCGG CACATCCTAC 
CACAGTGCGG TGGCCACCAG GCAGCTGCTG GAGGAACTGA CCGAACTGCC CGTTATGGTG 
GAACTGGCCT CCGATTTTCT GGACAGAAAC ACGCCCATTT TCCGGGACGA CGTGTGCTTC 
TTCATCTCCC AATCGGGCGA GACAGCGGAC ACCCTGATGG CGCTCAGATA CTGCAAGCAG 
CGAGGAGCGC TCATCGTGGG CGTTACCAAC ACGGTGGGCA GCAGCATCTG CCGCGAGTCG 
CACTGTGGCG TCCACATCAA CGCTGGACCC GAGATCGGAG TGGCCTCCAC GAAGGCCTAC 
ACCTCCCAGT TCATTTCGCT GGTTATGTTC GCCTTGGTCA TGTCGGAGGA TCGTCTCTCG 
CTGCAACAGC GCCGCTTGGA GATCATCGAT GGCCTATCGC AGCTGGACGA GCACATTAGG 
ACTGTCCTCA AGCTGAACTC GCAGGTGCAG CAGCTTGCCA AGGAGTTGTA CGAGCACAAG 
TCGCTGCTCA TTATGGGCAG GGGCTTCAAC TTCGCCACCT GTTTGGAGGG CGCACTGAAA 
GTCAAGGAGC TGACGTACAT GCACAGCGAA GGAATCCTGG CTGGAGAACT GAAACACGGA 
CCACTGGCCC TGGTGGACAA GGAGATGCCC GTGCTGATGA TCGTGCTGCG GGATCCGGTG 
TACACCAAGT GCATGAATGC ACTGCAGCAG GTGACGTCCC GCAAGGGCAG ACCGATCCTA 
ATCTGCGAGG AGGGCGACAA CGAGACCATG TCCTTCTCCA CGCGTTCGCT GCAGATCCCG 
AGGACCGTGG ACTGCCTGCA GGGCGTGCTC ACGGTGATTC CGCTCCAGCT GCTGTCCTAC 
CACATCGCCG TGCTCCGCGG CTGCGACGTG GACTGCCCCA GGAACCTGGC CAAGTCGGTG 
ACCGTGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651617.1
CDS	220..2271
Translation	MCGIFAYLNYLTPKSRQEVLDLLLQGLKRLEYRGYDSTGIAIDALNSGEAQSIMLVKRTGKVKVLEDAVAEVCRGQDYSLPIDTHIGIAHTRWATHGVPSEVNSHPQRSDEDNSFVVVHNGIITNYKDVKTLLEKRGYVFESDTDTEVIAKLVHHLWQQHPGYTFGELVEQAIQQLEGAFAIAFKSKHFPGECVASRRGSPLLVGIKAKTKLATDHIPILYAKAHRPHGQPQQQAYQLLPPGDCNAEFQPLERKEVEYFFASDASAVIEHTNRVIYLEDDDVAAVKSDGTLSIHRLNKSSDDPHAREIITLKMEIQQIMKGNYDYFMLKEIFEQPESVVNTMRGRMRFDTQTVVLGGIKEYIPEIKRCRRLMLIACGTSYHSAVATRQLLEELTELPVMVELASDFLDRNTPIFRDDVCFFISQSGETADTLMALRYCKQRGALIVGVTNTVGSSICRESHCGVHINAGPEIGVASTKAYTSQFISLVMFALVMSEDRLSLQQRRLEIIDGLSQLDEHIRTVLKLNSQVQQLAKELYEHKSLLIMGRGFNFATCLEGALKVKELTYMHSEGILAGELKHGPLALVDKEMPVLMIVLRDPVYTKCMNALQQVTSRKGRPILICEEGDNETMSFSTRSLQIPRTVDCLQGVLTVIPLQLLSYHIAVLRGCDVDCPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_143360.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamine:fructose-6-phosphate aminotransferase 2 (Gfat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143360.4

ORF Insert Sequence:

ATGTGTGGCA TATTCGCGTA CCTAAACTAC TTGACGCCCA AGTCGCGACA GGAGGTCCTG 
GATCTCCTGT TGCAGGGACT GAAGCGGCTG GAGTACCGCG GCTACGACTC CACGGGCATA 
GCCATCGATG CGCTGAACTC TGGCGAGGCA CAGTCCATAA TGCTGGTGAA GCGCACCGGG 
AAGGTGAAGG TGCTGGAGGA TGCGGTGGCG GAGGTGTGTC GCGGCCAGGA CTACTCGCTG 
CCCATCGACA CCCACATAGG CATAGCACAC ACGCGCTGGG CCACCCACGG TGTGCCCTCC 
GAGGTCAACT CGCATCCGCA GCGATCCGAT GAGGATAATA GCTTTGTGGT GGTGCACAAT 
GGCATTATAA CCAACTACAA GGATGTGAAG ACGCTGCTGG AGAAGCGCGG ATATGTATTT 
GAGTCCGACA CGGACACGGA GGTGATTGCC AAGCTGGTAC ACCACCTGTG GCAGCAGCAT 
CCTGGCTACA CTTTCGGCGA GCTCGTGGAG CAGGCCATTC AGCAGCTGGA GGGCGCCTTC 
GCCATCGCCT TCAAGTCGAA GCACTTCCCG GGCGAGTGCG TGGCCTCCAG GCGAGGCTCA 
CCGCTTCTGG TGGGCATCAA GGCCAAGACC AAGCTGGCCA CCGATCACAT ACCCATCCTG 
TACGCCAAGG CCCATCGGCC ACATGGTCAG CCGCAGCAGC AGGCCTATCA ACTATTGCCG 
CCCGGCGATT GCAACGCCGA GTTTCAACCG CTGGAGCGCA AGGAAGTGGA GTACTTCTTC 
GCCTCGGACG CATCGGCTGT TATAGAGCAC ACGAATCGGG TGATTTACCT GGAAGATGAC 
GACGTGGCCG CCGTCAAGAG CGATGGCACG CTCAGCATTC ATCGCCTAAA CAAATCATCC 
GATGATCCGC ACGCCCGCGA GATCATCACG CTGAAAATGG AGATCCAGCA GATCATGAAG 
GGCAACTACG ACTACTTTAT GCTGAAGGAG ATCTTCGAGC AGCCGGAATC CGTGGTCAAC 
ACCATGCGCG GCAGGATGAG GTTCGATACG CAGACCGTGG TTCTGGGTGG CATCAAGGAG 
TACATACCGG AGATCAAGCG CTGCCGCCGC TTGATGCTAA TTGCGTGCGG CACATCCTAC 
CACAGTGCGG TGGCCACCAG GCAGCTGCTG GAGGAACTGA CCGAACTGCC CGTTATGGTG 
GAACTGGCCT CCGATTTTCT GGACAGAAAC ACGCCCATTT TCCGGGACGA CGTGTGCTTC 
TTCATCTCCC AATCGGGCGA GACAGCGGAC ACCCTGATGG CGCTCAGATA CTGCAAGCAG 
CGAGGAGCGC TCATCGTGGG CGTTACCAAC ACGGTGGGCA GCAGCATCTG CCGCGAGTCG 
CACTGTGGCG TCCACATCAA CGCTGGACCC GAGATCGGAG TGGCCTCCAC GAAGGCCTAC 
ACCTCCCAGT TCATTTCGCT GGTTATGTTC GCCTTGGTCA TGTCGGAGGA TCGTCTCTCG 
CTGCAACAGC GCCGCTTGGA GATCATCGAT GGCCTATCGC AGCTGGACGA GCACATTAGG 
ACTGTCCTCA AGCTGAACTC GCAGGTGCAG CAGCTTGCCA AGGAGTTGTA CGAGCACAAG 
TCGCTGCTCA TTATGGGCAG GGGCTTCAAC TTCGCCACCT GTTTGGAGGG CGCACTGAAA 
GTCAAGGAGC TGACGTACAT GCACAGCGAA GGAATCCTGG CTGGAGAACT GAAACACGGA 
CCACTGGCCC TGGTGGACAA GGAGATGCCC GTGCTGATGA TCGTGCTGCG GGATCCGGTG 
TACACCAAGT GCATGAATGC ACTGCAGCAG GTGACGTCCC GCAAGGGCAG ACCGATCCTA 
ATCTGCGAGG AGGGCGACAA CGAGACCATG TCCTTCTCCA CGCGTTCGCT GCAGATCCCG 
AGGACCGTGG ACTGCCTGCA GGGCGTGCTC ACGGTGATTC CGCTCCAGCT GCTGTCCTAC 
CACATCGCCG TGCTCCGCGG CTGCGACGTG GACTGCCCCA GGAACCTGGC CAAGTCGGTG 
ACCGTGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gfat2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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