Gfpt2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfpt2
Entrez Gene ID	14584
Full Name	glutamine fructose-6-phosphate transaminase 2
Synonyms	AI480523,GFAT2
General protein information	Preferred Names glutamine fructose-6-phosphate transaminase 2 Names glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 D-fructose-6-phosphate amidotransferase 2 GFAT 2 glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 glutamine:fructose-6-phosphate amidotransferase 2 hexosephosphate aminotransferase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B1.2\|11 29.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013529.3	NP_038557.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GFPT2	XP_003642114.1
Drosophila melanogaster (fruit fly)	Gfat1	NP_001015157.2
Saccharomyces cerevisiae (baker's yeast)	GFA1	NP_012818.1
Eremothecium gossypii	AGOS_ABL036C	NP_982911.2
Oryza sativa (rice)	Os12g0131100	NP_001066074.1
	GFPT2	XP_001106226.2
Canis lupus familiaris (dog)	GFPT2	XP_005626343.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011352	XP_309298.2
Neurospora crassa	NCU11350	XP_957994.2
Pan troglodytes (chimpanzee)	GFPT2	XP_518158.4
Rattus norvegicus (Norway rat)	Gfpt2	NP_001002819.2
Caenorhabditis elegans (roundworm)	F22B3.4	NP_502156.1
Arabidopsis thaliana (thale cress)	AT3G24090	NP_189051.3
Drosophila melanogaster (fruit fly)	Gfat2	NP_651617.1
Caenorhabditis elegans (roundworm)	F07A11.2	NP_496479.1
Kluyveromyces lactis	KLLA0F13596g	XP_455692.1
Homo sapiens (human)	GFPT2	NP_005101.1
Bos taurus (cattle)	GFPT2	NP_001070351.1
Mus musculus (house mouse)	Gfpt2	NP_038557.1
Danio rerio (zebrafish)	gfpt2	NP_001029093.1
Schizosaccharomyces pombe (fission yeast)	SPBC12C2.11	NP_596011.1
Magnaporthe oryzae (rice blast fungus)	MGG_11597	XP_003718755.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

Differential mRNA processing in hematopoietic stem cells.
Bowman TV, McCooey AJ, Merchant AA, Ramos CA, Fonseca P, Poindexter A, Bradfute SB, Oliveira DM, Green R, Zheng Y, Jackson KA, Chambers SM, McKinney-Freeman SL, Norwood KG, Darlington G, Gunaratne PH, Steffen D, Goodell MA
Stem cells (Dayton, Ohio)24(3)662-70(2006 Mar)

Nalp1b controls mouse macrophage susceptibility to anthrax lethal toxin.
Boyden ED, Dietrich WF
Nature genetics38(2)240-4(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mouse glutamine-fructose-6-phosphate amidotransferase 2 (GFAT2) is phosphorylated by cAMP-dependent protein kinase, which increases the enzyme activity
Title: Phosphorylation of mouse glutamine-fructose-6-phosphate amidotransferase 2 (GFAT2) by cAMP-dependent protein kinase increases the enzyme activity.

The following Gfpt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfpt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09549	NM_013529.3 Latest version!	Mus musculus glutamine fructose-6-phosphate transaminase 2 (Gfpt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfpt2 ( NM_013529.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038557.1 Mus musculus glutamine fructose-6-phosphate transaminase 2 (Gfpt2), mRNA.

Price & Availability↑

CloneID	OMu09549
Clone ID Related Accession (Same CDS sequence)	NM_013529.3
Accession Version	NM_013529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ163487.1, AK132821.1, DY247913.1, BE198515.1 and BM204562.2. On Apr 16, 2009 this sequence version replaced NM_013529.2. ##Evidence-Data-START## Transcript exon combination :: BC031928.1, SRR1660815.213604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCGGAA TCTTTGCCTA CATGAATTAC AGAGTTCCCA AGACAAGGAA AGAGATTTTC 
GAAACCCTTA TCAGGGGTCT GCAGCGGCTG GAGTACCGGG GCTATGACTC TGCGGGGGTT 
GCCATTGATG GGAATAACCA CGAAGTCAAA GAAAGACACA TCCATCTTGT GAAGAAAAGG 
GGGAAAGTAA AGGCTCTGGA TGAAGAACTT TACAAGCAAG ATAGCATGGA CTTGAAGGTG 
GAGTTTGAGA CACACTTCGG CATTGCCCAC ACACGTTGGG CCACCCACGG GGTTCCCAAT 
GCTGTCAACA GTCACCCGCA GCGTTCGGAC AAAGACAATG AATTTGTTGT CATCCACAAC 
GGGATCATCA CTAATTACAA GGATCTAAGG AAGTTTCTGG AAAGCAAAGG CTACGAGTTT 
GAGTCAGAAA CAGACACGGA GACCATCGCC AAGCTGATTA AATATGTATT TGACAACAGA 
GAGACTGAGG ACATAACGTT TTCCACATTG GTCGAAAGAG TCATTCAGCA GTTGGAAGGC 
GCCTTTGCAC TGGTTTTCAA GAGTATTCAC TACCCGGGAG AAGCTGTCGC CACGAGGAGA 
GGCAGCCCCT TGCTCATCGG GGTACGAAGC AAATACAAAC TCTCCACAGA GCAGATCCCC 
GTCTTATATC CGACATGCAA TATCGAGAAT GTGAAGAATA TCTGCAAGAC TAGGATGAAG 
AGACTGGACA GCTCCACCTG CCTGCACGCT GTGGGCGATA AAGCTGTGGA ATTCTTCTTT 
GCTTCTGATG CAAGTGCCAT CATAGAACAC ACCAACCGGG TCATCTTCTT AGAAGATGAT 
GATATCGCTG CAGTGGCTGA TGGGAAACTC TCCATTCACC GAGTCAAGCG CTCAGCTACT 
GATGACCCCT CCCGAGCCAT CCAGACCTTG CAGATGGAAC TGCAGCAAAT AATGAAAGGT 
AACTTCAGCG CATTTATGCA GAAGGAGATC TTCGAGCAGC CAGAATCAGT TTTTAATACC 
ATGAGAGGTC GGGTGAATTT TGAGACCAAC ACAGTGCTCC TGGGTGGCTT GAAGGACCAT 
TTGAAAGAGA TCCGACGATG CCGAAGGCTC ATTGTGATTG GCTGTGGAAC CAGCTACCAT 
GCCGCTGTGG CTACACGGCA AGTCTTAGAG GAACTGACCG AGCTGCCTGT GATGGTTGAA 
CTTGCCAGTG ACTTTCTGGA CAGGAACACA CCTGTGTTCA GGGATGACGT TTGCTTTTTC 
ATAAGCCAAT CAGGTGAGAC TGCAGACACG CTCCTGGCGC TGCGATACTG TAAGGATCGA 
GGTGCGCTGA CCGTGGGCAT CACCAACACC GTGGGTAGCT CCATCTCCCG GGAGACTGAC 
TGTGGCGTCC ACATCAACGC AGGGCCCGAG ATTGGGGTGG CCAGCACCAA GGCGTACACC 
AGCCAGTTCA TCTCTCTGGT GATGTTTGGT TTGATGATGT CTGAAGATCG AATTTCTCTA 
CAGAACAGGA GACAAGAGAT CATCCGTGGC CTCAGATCTT TACCGGAGCT GATCAAAGAA 
GTGCTGTCCC TGGATGAGAA GATCCATGAC TTGGCCCTGG AGCTCTACAC ACAAAGGTCT 
CTCCTCGTGA TGGGACGGGG ATATAACTAT GCCACATGTC TGGAAGGTGC CTTGAAAATT 
AAGGAGATAA CCTACATGCA TTCAGAAGGT ATCCTAGCCG GAGAGCTGAA GCACGGGCCC 
CTTGCTCTCG TCGACAAGCA GATGCCAGTC ATCATGGTCA TCATGAAGGA TCCTTGCTTT 
GCCAAGTGCC AGAATGCCCT GCAGCAGGTC ACTGCCCGCC AGGGTCGCCC AATCATACTG 
TGTTCCAAGG ATGACACCGA GAGCTCCAAG TTTGCATATA AAACCATTGA ACTTCCCCAC 
ACAGTGGACT GTCTCCAGGG TATCCTGAGC GTGATTCCAC TCCAGCTTCT GTCCTTCCAC 
CTGGCTGTCC TCCGAGGTTA TGATGTTGAC TTCCCCAGAA ACCTAGCCAA GTCTGTCACT 
GTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038557.1
CDS	83..2131
Translation	MCGIFAYMNYRVPKTRKEIFETLIRGLQRLEYRGYDSAGVAIDGNNHEVKERHIHLVKKRGKVKALDEELYKQDSMDLKVEFETHFGIAHTRWATHGVPNAVNSHPQRSDKDNEFVVIHNGIITNYKDLRKFLESKGYEFESETDTETIAKLIKYVFDNRETEDITFSTLVERVIQQLEGAFALVFKSIHYPGEAVATRRGSPLLIGVRSKYKLSTEQIPVLYPTCNIENVKNICKTRMKRLDSSTCLHAVGDKAVEFFFASDASAIIEHTNRVIFLEDDDIAAVADGKLSIHRVKRSATDDPSRAIQTLQMELQQIMKGNFSAFMQKEIFEQPESVFNTMRGRVNFETNTVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFISLVMFGLMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLDEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_013529.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamine fructose-6-phosphate transaminase 2 (Gfpt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013529.3

ORF Insert Sequence:

ATGTGCGGAA TCTTTGCCTA CATGAATTAC AGAGTTCCCA AGACAAGGAA AGAGATTTTC 
GAAACCCTTA TCAGGGGTCT GCAGCGGCTG GAGTACCGGG GCTATGACTC TGCGGGGGTT 
GCCATTGATG GGAATAACCA CGAAGTCAAA GAAAGACACA TCCATCTTGT GAAGAAAAGG 
GGGAAAGTAA AGGCTCTGGA TGAAGAACTT TACAAGCAAG ATAGCATGGA CTTGAAGGTG 
GAGTTTGAGA CACACTTCGG CATTGCCCAC ACACGTTGGG CCACCCACGG GGTTCCCAAT 
GCTGTCAACA GTCACCCGCA GCGTTCGGAC AAAGACAATG AATTTGTTGT CATCCACAAC 
GGGATCATCA CTAATTACAA GGATCTAAGG AAGTTTCTGG AAAGCAAAGG CTACGAGTTT 
GAGTCAGAAA CAGACACGGA GACCATCGCC AAGCTGATTA AATATGTATT TGACAACAGA 
GAGACTGAGG ACATAACGTT TTCCACATTG GTCGAAAGAG TCATTCAGCA GTTGGAAGGC 
GCCTTTGCAC TGGTTTTCAA GAGTATTCAC TACCCGGGAG AAGCTGTCGC CACGAGGAGA 
GGCAGCCCCT TGCTCATCGG GGTACGAAGC AAATACAAAC TCTCCACAGA GCAGATCCCC 
GTCTTATATC CGACATGCAA TATCGAGAAT GTGAAGAATA TCTGCAAGAC TAGGATGAAG 
AGACTGGACA GCTCCACCTG CCTGCACGCT GTGGGCGATA AAGCTGTGGA ATTCTTCTTT 
GCTTCTGATG CAAGTGCCAT CATAGAACAC ACCAACCGGG TCATCTTCTT AGAAGATGAT 
GATATCGCTG CAGTGGCTGA TGGGAAACTC TCCATTCACC GAGTCAAGCG CTCAGCTACT 
GATGACCCCT CCCGAGCCAT CCAGACCTTG CAGATGGAAC TGCAGCAAAT AATGAAAGGT 
AACTTCAGCG CATTTATGCA GAAGGAGATC TTCGAGCAGC CAGAATCAGT TTTTAATACC 
ATGAGAGGTC GGGTGAATTT TGAGACCAAC ACAGTGCTCC TGGGTGGCTT GAAGGACCAT 
TTGAAAGAGA TCCGACGATG CCGAAGGCTC ATTGTGATTG GCTGTGGAAC CAGCTACCAT 
GCCGCTGTGG CTACACGGCA AGTCTTAGAG GAACTGACCG AGCTGCCTGT GATGGTTGAA 
CTTGCCAGTG ACTTTCTGGA CAGGAACACA CCTGTGTTCA GGGATGACGT TTGCTTTTTC 
ATAAGCCAAT CAGGTGAGAC TGCAGACACG CTCCTGGCGC TGCGATACTG TAAGGATCGA 
GGTGCGCTGA CCGTGGGCAT CACCAACACC GTGGGTAGCT CCATCTCCCG GGAGACTGAC 
TGTGGCGTCC ACATCAACGC AGGGCCCGAG ATTGGGGTGG CCAGCACCAA GGCGTACACC 
AGCCAGTTCA TCTCTCTGGT GATGTTTGGT TTGATGATGT CTGAAGATCG AATTTCTCTA 
CAGAACAGGA GACAAGAGAT CATCCGTGGC CTCAGATCTT TACCGGAGCT GATCAAAGAA 
GTGCTGTCCC TGGATGAGAA GATCCATGAC TTGGCCCTGG AGCTCTACAC ACAAAGGTCT 
CTCCTCGTGA TGGGACGGGG ATATAACTAT GCCACATGTC TGGAAGGTGC CTTGAAAATT 
AAGGAGATAA CCTACATGCA TTCAGAAGGT ATCCTAGCCG GAGAGCTGAA GCACGGGCCC 
CTTGCTCTCG TCGACAAGCA GATGCCAGTC ATCATGGTCA TCATGAAGGA TCCTTGCTTT 
GCCAAGTGCC AGAATGCCCT GCAGCAGGTC ACTGCCCGCC AGGGTCGCCC AATCATACTG 
TGTTCCAAGG ATGACACCGA GAGCTCCAAG TTTGCATATA AAACCATTGA ACTTCCCCAC 
ACAGTGGACT GTCTCCAGGG TATCCTGAGC GTGATTCCAC TCCAGCTTCT GTCCTTCCAC 
CTGGCTGTCC TCCGAGGTTA TGATGTTGAC TTCCCCAGAA ACCTAGCCAA GTCTGTCACT 
GTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

Differential mRNA processing in hematopoietic stem cells.
Stem cells (Dayton, Ohio)24(3)662-70(2006 Mar)
Bowman TV,McCooey AJ,Merchant AA,Ramos CA,Fonseca P,Poindexter A,Bradfute SB,Oliveira DM,Green R,Zheng Y,Jackson KA,Chambers SM,McKinney-Freeman SL,Norwood KG,Darlington G,Gunaratne PH,Steffen D,Goodell MA

Nalp1b controls mouse macrophage susceptibility to anthrax lethal toxin.
Nature genetics38(2)240-4(2006 Feb)
Boyden ED,Dietrich WF