Smg6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg6
Entrez Gene ID	42994
Full Name	CG6369 gene product from transcript CG6369-RA
Synonyms	CG6369,Dmel\CG6369,SMG6,dSMG6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B17-96B17

mRNA and Protein(s)

mRNA	Protein	Name
NM_143064.2	NP_651321.1	Smg6

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000894	XP_316872.5
Drosophila melanogaster (fruit fly)	Smg6	NP_651321.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SMG6 is a conserved endonuclease that degrades mRNAs terminating translation prematurely in metazoa
Title: SMG6 is the catalytic endonuclease that cleaves mRNAs containing nonsense codons in metazoan.

The following Smg6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08787	NM_143064.2 Latest version!	Drosophila melanogaster Smg6 (Smg6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg6 ( NM_143064.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651321.1 Drosophila melanogaster Smg6 (Smg6), mRNA.

Price & Availability↑

CloneID	OFa08787
Clone ID Related Accession (Same CDS sequence)	NM_143064.2
Accession Version	NM_143064.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Smg6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143064.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGAA AGGGAACACG AGAACAAAAT GATATATCGC TGATAATTGG GACGCTGCCG 
AAACGCCTGC AGAACAAAAT CGCGGCCAGC TGCAGCCACG CAGCAGCAGC GGCAACAACA 
ACAAAGGCGT TGGAGACGGA AAACGCTGAG AATGCGGAGC CCGTGGCTAC GACAGCAAAT 
GCTTCAAGGC CAGTCGAAAT CCTGAGCAAT GCTAGCACAG GAGGAGGCAA ACCCGATGCC 
CAGTCAAAGC AACCCGTTTT GGATACCAGC GGGAAAATGG CGTGGGAGGG CGGGATTCCA 
GGCATCCTGC GCGTGACCAC CTCGGTGGCG GGCGATGTTA ACAGGAACGG ACAGGGTCAG 
GGCAAGAACA AGAGCGGTAA CAGTGCTCGG CGCAGGGGTC GCAAGGACAA GGAGCACGCT 
ACGCAGTCGC CAGTGACGCG CACGCGCTTG ACCACCGAGC ATCCGGATTG GTTTGAGCCG 
CTGTTGGGTC TGCCCCATGC CAAGAATATA GTCCATCTGT ACGATCAGCT CATGGTCCTC 
AATCAGAGGA ATCTCATACT GATAAACTGG AAGAACTTCT GCTACATTCA CGACCAGCTG 
CAGGACGCTT TCAAGCAACT TCTTTTGGAG CAACTTAAGT TCGTGTGCGA GCACAAGGTT 
GATGTCTTCT TTTGGAAACT ACTCTTCTAC AATGTGCGGA ATTACTTGAA GCGCCAGCAA 
ACGGACCAGG CGCACACACA TACGCTCCTT TTGATTGAAC AGGCCATTAA GTTTTACCGC 
ATGCTCTACG ACAAGCTGAT GGCCAAGTGC GTGGCATCGT CGCGCTGTGA CAGCGCACTT 
AAAGTGGTGG CCCAGAGGCT GCTCATCTGT TTGGGCGATC TGACGCGCTA CCGGGTTAAC 
CATGTCAAGG CCACAGATTA CATGGAGGCG GCCAGGTACT ATCAGAGGGC GCAGGAACTG 
GTACCTGGCA ACGGGGCACC TTTCAACCAG CTGGCGGTCA TTTCCATTTA TCACCACAAG 
CGCTTCGATG CAGTTTATTA CTATGTCCGC AGCTTGTTGA CTTCGAACTC TATCCAATCT 
GCCAAGGAAA GCCTGCTGGA TTTGTTCGAC GAGATTCGGC GTAAGTACGA GGAGACCGAG 
ATGAAGCAAT CTCCCATGCA CTATGCAGCT GGATCGAAGA ATCAAAAGTC GAAGCAGATG 
CGCAAGGAGG TATGGATCTA TCCGGATGGG ATCAGACGCC TACATCGCAC CGATGACAAG 
GGCAACAAGG CGAAGGGCAA CAAGGCCACT ATGGCAGAGG TCAATCGTTA CGAGGAGATG 
CCGCCGGAGG AGATTCTTCC TCGCATTGTT TCCCTTTATC TGTACTTATT AGGCAAACTC 
TACACCGGCA CAGATGTGGA CTCGTTATAC CCGTTGCTAA GGAAACTGCA AATCCAAATA 
GGCGTCGCTT TAAGGCACGA AAACCTGTTG TCACGCTCCA AACTGCTGAA AATAGTAGCA 
CTCAACTTGT TCGTCGTCGA GCACAACAAA CCCAAAACGT CACGCCGCGA GATGCGTTAT 
CACAGCTTCA ATTTTGCCAA CTCATTCTTT GGACTCATGA TGAAGAAAAC GAACCAGCTG 
CTGGCTGGTT TTGTAGAGGA TTCTACAAAT GTCCAGTGTC TCCCAGAAGA AGACTTTGCG 
ACTGTGAACA CATACCTACA GTTCGTGAAT GTTTATGTAC GTTGGTTGAG CATCAGTTTG 
GACGTGTGGG AACCCGTCAG ATCGGAAGAA CACTCTTTCA TCGATTGCTG GGCTGAGCTA 
ACAATCCTTT TTGAGTACAT TGAATGCTTA ATGGGGAAGC ACAAACTTGA GAAAAACGAA 
ATACTGTTGG ATGAGGATGT GGCCCTTAGA GGTTTCACTC CGTTGGGTCG TGAAACGCTG 
AGCATGGACT ATCTCAAAAG AGGATCCGAA CGTCTTCAGT TTTTCGAACG TGTTCGTAGG 
ATTGGTCAGT TCCAGGAATA CTACGTCCAG CATCAAGAGG CTCTCCAGCA GCCGTTGGAA 
TCAAGCTTCA CTGTCGAGCA CCTAAACGCA GCGATGAAGA ACGCATTGGA AAACTATGAT 
GCTGAAAGTT GTGGTCTTGC GGCGAACGTT TCAAAGATTC AATGTAAAGC CGTTGAGGAA 
TTGTTGTGTC GTTCAACTGA TTCCGATGTT TCCGAGCTGT GCAAGCTGAA GAAAGAGCTT 
GAGGCCAAGG CTAGGGTTAA GCAGATATGC ACCAGCAAGT TGGAGGACAT TCTCAAGTTC 
GTGGACACGA AAATATACAT CGAGGTGCGA CCGCGCTATC TTCTACCGGA TACCAACTGC 
TTTATCGACT GCCTTGAAGA CTTTGAAAAA CTGTCCACGG AGTTTAAACG CTACACACTT 
ATTATACCAC TGACAGTGGT TAAGGAACTG GATGGCCTCT CCAAGGGCGT TAAGTTGGAC 
TCGTACCGCA GTTCGAAGCA GACGCAGCGC ATCCACCACT TTGACGAGGT GTCGTCGCGG 
GCCAAAAAGT CGTTAGAGTT CATCAAGTCG GCCAAAGGCA ATGTGAAATG TGCTACCACA 
AAGGGCTCGT TTGTCAATGC ATCGGTGTTT GCCCTAGTTG AGGAGGAATA TTTGTCCAAT 
GACGACAAGA TTCTGGCAAC GGCAATGGCT GTCTCGAAAA CGGCAAAAAC CGAACAATGC 
ACAGATGGCA AGTGTTTCAT TCAAACGGAA TTGGTGCTCA TTACAACCGA TCGTAATCTG 
CGTGTGAAGG CTCTTTCTCG CAATTTGGCC GTCAGCGCGT TGGATGAGTT TCTGCAATGG 
GCCAAGGATT GCCGCGAATC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651321.1
CDS	114..2960
Translation	MSRKGTREQNDISLIIGTLPKRLQNKIAASCSHAAAAATTTKALETENAENAEPVATTANASRPVEILSNASTGGGKPDAQSKQPVLDTSGKMAWEGGIPGILRVTTSVAGDVNRNGQGQGKNKSGNSARRRGRKDKEHATQSPVTRTRLTTEHPDWFEPLLGLPHAKNIVHLYDQLMVLNQRNLILINWKNFCYIHDQLQDAFKQLLLEQLKFVCEHKVDVFFWKLLFYNVRNYLKRQQTDQAHTHTLLLIEQAIKFYRMLYDKLMAKCVASSRCDSALKVVAQRLLICLGDLTRYRVNHVKATDYMEAARYYQRAQELVPGNGAPFNQLAVISIYHHKRFDAVYYYVRSLLTSNSIQSAKESLLDLFDEIRRKYEETEMKQSPMHYAAGSKNQKSKQMRKEVWIYPDGIRRLHRTDDKGNKAKGNKATMAEVNRYEEMPPEEILPRIVSLYLYLLGKLYTGTDVDSLYPLLRKLQIQIGVALRHENLLSRSKLLKIVALNLFVVEHNKPKTSRREMRYHSFNFANSFFGLMMKKTNQLLAGFVEDSTNVQCLPEEDFATVNTYLQFVNVYVRWLSISLDVWEPVRSEEHSFIDCWAELTILFEYIECLMGKHKLEKNEILLDEDVALRGFTPLGRETLSMDYLKRGSERLQFFERVRRIGQFQEYYVQHQEALQQPLESSFTVEHLNAAMKNALENYDAESCGLAANVSKIQCKAVEELLCRSTDSDVSELCKLKKELEAKARVKQICTSKLEDILKFVDTKIYIEVRPRYLLPDTNCFIDCLEDFEKLSTEFKRYTLIIPLTVVKELDGLSKGVKLDSYRSSKQTQRIHHFDEVSSRAKKSLEFIKSAKGNVKCATTKGSFVNASVFALVEEEYLSNDDKILATAMAVSKTAKTEQCTDGKCFIQTELVLITTDRNLRVKALSRNLAVSALDEFLQWAKDCREST

Target ORF information:

RefSeq Version	NM_143064.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Smg6 (Smg6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143064.2

ORF Insert Sequence:

ATGAGTCGAA AGGGAACACG AGAACAAAAT GATATATCGC TGATAATTGG GACGCTGCCG 
AAACGCCTGC AGAACAAAAT CGCGGCCAGC TGCAGCCACG CAGCAGCAGC GGCAACAACA 
ACAAAGGCGT TGGAGACGGA AAACGCTGAG AATGCGGAGC CCGTGGCTAC GACAGCAAAT 
GCTTCAAGGC CAGTCGAAAT CCTGAGCAAT GCTAGCACAG GAGGAGGCAA ACCCGATGCC 
CAGTCAAAGC AACCCGTTTT GGATACCAGC GGGAAAATGG CGTGGGAGGG CGGGATTCCA 
GGCATCCTGC GCGTGACCAC CTCGGTGGCG GGCGATGTTA ACAGGAACGG ACAGGGTCAG 
GGCAAGAACA AGAGCGGTAA CAGTGCTCGG CGCAGGGGTC GCAAGGACAA GGAGCACGCT 
ACGCAGTCGC CAGTGACGCG CACGCGCTTG ACCACCGAGC ATCCGGATTG GTTTGAGCCG 
CTGTTGGGTC TGCCCCATGC CAAGAATATA GTCCATCTGT ACGATCAGCT CATGGTCCTC 
AATCAGAGGA ATCTCATACT GATAAACTGG AAGAACTTCT GCTACATTCA CGACCAGCTG 
CAGGACGCTT TCAAGCAACT TCTTTTGGAG CAACTTAAGT TCGTGTGCGA GCACAAGGTT 
GATGTCTTCT TTTGGAAACT ACTCTTCTAC AATGTGCGGA ATTACTTGAA GCGCCAGCAA 
ACGGACCAGG CGCACACACA TACGCTCCTT TTGATTGAAC AGGCCATTAA GTTTTACCGC 
ATGCTCTACG ACAAGCTGAT GGCCAAGTGC GTGGCATCGT CGCGCTGTGA CAGCGCACTT 
AAAGTGGTGG CCCAGAGGCT GCTCATCTGT TTGGGCGATC TGACGCGCTA CCGGGTTAAC 
CATGTCAAGG CCACAGATTA CATGGAGGCG GCCAGGTACT ATCAGAGGGC GCAGGAACTG 
GTACCTGGCA ACGGGGCACC TTTCAACCAG CTGGCGGTCA TTTCCATTTA TCACCACAAG 
CGCTTCGATG CAGTTTATTA CTATGTCCGC AGCTTGTTGA CTTCGAACTC TATCCAATCT 
GCCAAGGAAA GCCTGCTGGA TTTGTTCGAC GAGATTCGGC GTAAGTACGA GGAGACCGAG 
ATGAAGCAAT CTCCCATGCA CTATGCAGCT GGATCGAAGA ATCAAAAGTC GAAGCAGATG 
CGCAAGGAGG TATGGATCTA TCCGGATGGG ATCAGACGCC TACATCGCAC CGATGACAAG 
GGCAACAAGG CGAAGGGCAA CAAGGCCACT ATGGCAGAGG TCAATCGTTA CGAGGAGATG 
CCGCCGGAGG AGATTCTTCC TCGCATTGTT TCCCTTTATC TGTACTTATT AGGCAAACTC 
TACACCGGCA CAGATGTGGA CTCGTTATAC CCGTTGCTAA GGAAACTGCA AATCCAAATA 
GGCGTCGCTT TAAGGCACGA AAACCTGTTG TCACGCTCCA AACTGCTGAA AATAGTAGCA 
CTCAACTTGT TCGTCGTCGA GCACAACAAA CCCAAAACGT CACGCCGCGA GATGCGTTAT 
CACAGCTTCA ATTTTGCCAA CTCATTCTTT GGACTCATGA TGAAGAAAAC GAACCAGCTG 
CTGGCTGGTT TTGTAGAGGA TTCTACAAAT GTCCAGTGTC TCCCAGAAGA AGACTTTGCG 
ACTGTGAACA CATACCTACA GTTCGTGAAT GTTTATGTAC GTTGGTTGAG CATCAGTTTG 
GACGTGTGGG AACCCGTCAG ATCGGAAGAA CACTCTTTCA TCGATTGCTG GGCTGAGCTA 
ACAATCCTTT TTGAGTACAT TGAATGCTTA ATGGGGAAGC ACAAACTTGA GAAAAACGAA 
ATACTGTTGG ATGAGGATGT GGCCCTTAGA GGTTTCACTC CGTTGGGTCG TGAAACGCTG 
AGCATGGACT ATCTCAAAAG AGGATCCGAA CGTCTTCAGT TTTTCGAACG TGTTCGTAGG 
ATTGGTCAGT TCCAGGAATA CTACGTCCAG CATCAAGAGG CTCTCCAGCA GCCGTTGGAA 
TCAAGCTTCA CTGTCGAGCA CCTAAACGCA GCGATGAAGA ACGCATTGGA AAACTATGAT 
GCTGAAAGTT GTGGTCTTGC GGCGAACGTT TCAAAGATTC AATGTAAAGC CGTTGAGGAA 
TTGTTGTGTC GTTCAACTGA TTCCGATGTT TCCGAGCTGT GCAAGCTGAA GAAAGAGCTT 
GAGGCCAAGG CTAGGGTTAA GCAGATATGC ACCAGCAAGT TGGAGGACAT TCTCAAGTTC 
GTGGACACGA AAATATACAT CGAGGTGCGA CCGCGCTATC TTCTACCGGA TACCAACTGC 
TTTATCGACT GCCTTGAAGA CTTTGAAAAA CTGTCCACGG AGTTTAAACG CTACACACTT 
ATTATACCAC TGACAGTGGT TAAGGAACTG GATGGCCTCT CCAAGGGCGT TAAGTTGGAC 
TCGTACCGCA GTTCGAAGCA GACGCAGCGC ATCCACCACT TTGACGAGGT GTCGTCGCGG 
GCCAAAAAGT CGTTAGAGTT CATCAAGTCG GCCAAAGGCA ATGTGAAATG TGCTACCACA 
AAGGGCTCGT TTGTCAATGC ATCGGTGTTT GCCCTAGTTG AGGAGGAATA TTTGTCCAAT 
GACGACAAGA TTCTGGCAAC GGCAATGGCT GTCTCGAAAA CGGCAAAAAC CGAACAATGC 
ACAGATGGCA AGTGTTTCAT TCAAACGGAA TTGGTGCTCA TTACAACCGA TCGTAATCTG 
CGTGTGAAGG CTCTTTCTCG CAATTTGGCC GTCAGCGCGT TGGATGAGTT TCTGCAATGG 
GCCAAGGATT GCCGCGAATC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Smg6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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