Sirt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sirt2
Entrez Gene ID	42414
Full Name	Sirtuin 2
Synonyms	CG5085,D.mel2,Dmel\CG5085,SIRT2,dSIRT2
General protein information	Preferred Names Sirtuin 2 Names CG5085 gene product from transcript CG5085-RB CG5085-PA CG5085-PB Sirt2-PA Sirt2-PB sirtuin 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92E3-92E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300493.1	NP_001287422.1	sirtuin 2, isoform B
NM_142623.4	NP_650880.2	sirtuin 2, isoform A

Pathways from BioSystems

Homologies

	LOC100425019	XP_002801273.1
Drosophila melanogaster (fruit fly)	Sirt2	NP_650880.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002943	XP_311957.5
Schizosaccharomyces pombe (fission yeast)	hst2	NP_588147.1
Pan troglodytes (chimpanzee)	SIRT2	XP_001168375.1
Danio rerio (zebrafish)	sirt2	NP_955890.1
Bos taurus (cattle)	SIRT2	NP_001107003.1
Mus musculus (house mouse)	Sirt2	NP_071877.3
Rattus norvegicus (Norway rat)	Sirt2	NP_001008369.1
Canis lupus familiaris (dog)	SIRT2	XP_855382.2
Kluyveromyces lactis	KLLA0F11033g	XP_455583.1
Magnaporthe oryzae (rice blast fungus)	MGG_17827	XP_003720356.1
Xenopus tropicalis (tropical clawed frog)	LOC100489469	XP_002935827.2
Homo sapiens (human)	SIRT2	NP_036369.2
Gallus gallus (chicken)	SIRT2	NP_001017414.1
Saccharomyces cerevisiae (baker's yeast)	HST2	NP_015310.1
Eremothecium gossypii	AGOS_AGL018C	NP_986648.1
Neurospora crassa	NCU00523	XP_963725.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sirt2 has an role in the regulation of mitochondrial energy metabolism.
Title: Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase ? and regulates complex V activity.

The following Sirt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sirt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05464	NM_142623.4 Latest version!	Drosophila melanogaster sirtuin 2, transcript variant A (Sirt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05464	NM_001300493.1 Latest version!	Drosophila melanogaster sirtuin 2, transcript variant B (Sirt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sirt2 ( NM_001300493.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287422.1 Drosophila melanogaster sirtuin 2, transcript variant B (Sirt2), mRNA.

Price & Availability↑

CloneID	OFa05464
Clone ID Related Accession (Same CDS sequence)	NM_001300493.1 , NM_142623.4
Accession Version	NM_001300493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sirtuin 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAAGG TTCGACGCTT CTTTGCAAAC ACTCTACATC TCGGCGGCTC CTCGGACGCA 
AAGGAGGAAG TTAAAGTAGA GAAGGTTATT CCAGATTTGA GTTTTGACGG CTTTGCCGAA 
CATTGGCGTG TGCATGGTTT CCGCAAGATT GTGACCATGG TGGGAGCCGG CATATCTACA 
TCTGCTGGCA TTCCGGATTT CAGATCCCCA GGTTCCGGGT TATACAGCAA TCTGAAGAAG 
TACGAGCTGC CACATCCTAC GGCCATATTC GATCTGGACT ACTTCGAAAA GAATCCAGCG 
CCGTTTTTTG CACTGGCCAA GGAACTTTAC CCTGGATCCT TCATTCCCAC GCCGGCCCAC 
TACTTTATCC GTTTGCTTAA CGACAAGGGA CTACTGCAGC GCCACTACAC CCAGAACATA 
GACACGCTAG ACCGGCTTAC TGGCCTGCCC GAGGACAAGA TCATTGAGGC CCATGGCAGC 
TTCCACACCA ACCACTGCAT TAAGTGCCGC AAGGAGTACG ACATGGACTG GATGAAGGCG 
GAGATCTTCG CCGATCGTCT GCCCAAATGC CAAAAGTGCC AAGGCGTTGT TAAACCAGAC 
ATTGTTTTCT TTGGCGAAAA CTTGCCAAAG AGATTTTACT CCAGTCCTGA AGAGGATTTC 
CAGGATTGCG ATCTGCTGAT CATCATGGGC ACATCGTTGG AAGTGCAACC GTTTGCTTCA 
CTGGTGTGGA GACCAGGACC ACGTTGCATT CGCCTCTTGA TCAATCGCGA TGCAGTGGGT 
CAGGCCAGCT GTGTGCTCTT TATGGATCCC AACACGCGAT CGCTACTCTT CGATAAGCCC 
AATAACACTA GGGATGTGGC CTTTCTGGGC GACTGTGATG CTGGCGTAAT GGCTCTGGCC 
AAAGCCTTGG GCTGGGACCA AGAGCTGCAG CAGCTAATTA CAAGTGAAAG GAAGAAACTG 
AGCGGCAGCC AGAATAGTGA GGAGCTGCAA CAAGGCAAAG AGAAACCGCA ATCGGACCCG 
GATAAGATGA CTTCGGGCGA TAGGGACAAG AAGGATGCTT CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287422.1
CDS	218..1285
Translation	MDKVRRFFANTLHLGGSSDAKEEVKVEKVIPDLSFDGFAEHWRVHGFRKIVTMVGAGISTSAGIPDFRSPGSGLYSNLKKYELPHPTAIFDLDYFEKNPAPFFALAKELYPGSFIPTPAHYFIRLLNDKGLLQRHYTQNIDTLDRLTGLPEDKIIEAHGSFHTNHCIKCRKEYDMDWMKAEIFADRLPKCQKCQGVVKPDIVFFGENLPKRFYSSPEEDFQDCDLLIIMGTSLEVQPFASLVWRPGPRCIRLLINRDAVGQASCVLFMDPNTRSLLFDKPNNTRDVAFLGDCDAGVMALAKALGWDQELQQLITSERKKLSGSQNSEELQQGKEKPQSDPDKMTSGDRDKKDASL

Target ORF information:

RefSeq Version	NM_001300493.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sirtuin 2, transcript variant B (Sirt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300493.1

ORF Insert Sequence:

ATGGATAAGG TTCGACGCTT CTTTGCAAAC ACTCTACATC TCGGCGGCTC CTCGGACGCA 
AAGGAGGAAG TTAAAGTAGA GAAGGTTATT CCAGATTTGA GTTTTGACGG CTTTGCCGAA 
CATTGGCGTG TGCATGGTTT CCGCAAGATT GTGACCATGG TGGGAGCCGG CATATCTACA 
TCTGCTGGCA TTCCGGATTT CAGATCCCCA GGTTCCGGGT TATACAGCAA TCTGAAGAAG 
TACGAGCTGC CACATCCTAC GGCCATATTC GATCTGGACT ACTTCGAAAA GAATCCAGCG 
CCGTTTTTTG CACTGGCCAA GGAACTTTAC CCTGGATCCT TCATTCCCAC GCCGGCCCAC 
TACTTTATCC GTTTGCTTAA CGACAAGGGA CTACTGCAGC GCCACTACAC CCAGAACATA 
GACACGCTAG ACCGGCTTAC TGGCCTGCCC GAGGACAAGA TCATTGAGGC CCATGGCAGC 
TTCCACACCA ACCACTGCAT TAAGTGCCGC AAGGAGTACG ACATGGACTG GATGAAGGCG 
GAGATCTTCG CCGATCGTCT GCCCAAATGC CAAAAGTGCC AAGGCGTTGT TAAACCAGAC 
ATTGTTTTCT TTGGCGAAAA CTTGCCAAAG AGATTTTACT CCAGTCCTGA AGAGGATTTC 
CAGGATTGCG ATCTGCTGAT CATCATGGGC ACATCGTTGG AAGTGCAACC GTTTGCTTCA 
CTGGTGTGGA GACCAGGACC ACGTTGCATT CGCCTCTTGA TCAATCGCGA TGCAGTGGGT 
CAGGCCAGCT GTGTGCTCTT TATGGATCCC AACACGCGAT CGCTACTCTT CGATAAGCCC 
AATAACACTA GGGATGTGGC CTTTCTGGGC GACTGTGATG CTGGCGTAAT GGCTCTGGCC 
AAAGCCTTGG GCTGGGACCA AGAGCTGCAG CAGCTAATTA CAAGTGAAAG GAAGAAACTG 
AGCGGCAGCC AGAATAGTGA GGAGCTGCAA CAAGGCAAAG AGAAACCGCA ATCGGACCCG 
GATAAGATGA CTTCGGGCGA TAGGGACAAG AAGGATGCTT CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirt2 ( NM_142623.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650880.2 Drosophila melanogaster sirtuin 2, transcript variant A (Sirt2), mRNA.

Price & Availability↑

CloneID	OFa05464
Clone ID Related Accession (Same CDS sequence)	NM_001300493.1 , NM_142623.4
Accession Version	NM_142623.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sirtuin 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142623.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAAGG TTCGACGCTT CTTTGCAAAC ACTCTACATC TCGGCGGCTC CTCGGACGCA 
AAGGAGGAAG TTAAAGTAGA GAAGGTTATT CCAGATTTGA GTTTTGACGG CTTTGCCGAA 
CATTGGCGTG TGCATGGTTT CCGCAAGATT GTGACCATGG TGGGAGCCGG CATATCTACA 
TCTGCTGGCA TTCCGGATTT CAGATCCCCA GGTTCCGGGT TATACAGCAA TCTGAAGAAG 
TACGAGCTGC CACATCCTAC GGCCATATTC GATCTGGACT ACTTCGAAAA GAATCCAGCG 
CCGTTTTTTG CACTGGCCAA GGAACTTTAC CCTGGATCCT TCATTCCCAC GCCGGCCCAC 
TACTTTATCC GTTTGCTTAA CGACAAGGGA CTACTGCAGC GCCACTACAC CCAGAACATA 
GACACGCTAG ACCGGCTTAC TGGCCTGCCC GAGGACAAGA TCATTGAGGC CCATGGCAGC 
TTCCACACCA ACCACTGCAT TAAGTGCCGC AAGGAGTACG ACATGGACTG GATGAAGGCG 
GAGATCTTCG CCGATCGTCT GCCCAAATGC CAAAAGTGCC AAGGCGTTGT TAAACCAGAC 
ATTGTTTTCT TTGGCGAAAA CTTGCCAAAG AGATTTTACT CCAGTCCTGA AGAGGATTTC 
CAGGATTGCG ATCTGCTGAT CATCATGGGC ACATCGTTGG AAGTGCAACC GTTTGCTTCA 
CTGGTGTGGA GACCAGGACC ACGTTGCATT CGCCTCTTGA TCAATCGCGA TGCAGTGGGT 
CAGGCCAGCT GTGTGCTCTT TATGGATCCC AACACGCGAT CGCTACTCTT CGATAAGCCC 
AATAACACTA GGGATGTGGC CTTTCTGGGC GACTGTGATG CTGGCGTAAT GGCTCTGGCC 
AAAGCCTTGG GCTGGGACCA AGAGCTGCAG CAGCTAATTA CAAGTGAAAG GAAGAAACTG 
AGCGGCAGCC AGAATAGTGA GGAGCTGCAA CAAGGCAAAG AGAAACCGCA ATCGGACCCG 
GATAAGATGA CTTCGGGCGA TAGGGACAAG AAGGATGCTT CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650880.2
CDS	218..1285
Translation	MDKVRRFFANTLHLGGSSDAKEEVKVEKVIPDLSFDGFAEHWRVHGFRKIVTMVGAGISTSAGIPDFRSPGSGLYSNLKKYELPHPTAIFDLDYFEKNPAPFFALAKELYPGSFIPTPAHYFIRLLNDKGLLQRHYTQNIDTLDRLTGLPEDKIIEAHGSFHTNHCIKCRKEYDMDWMKAEIFADRLPKCQKCQGVVKPDIVFFGENLPKRFYSSPEEDFQDCDLLIIMGTSLEVQPFASLVWRPGPRCIRLLINRDAVGQASCVLFMDPNTRSLLFDKPNNTRDVAFLGDCDAGVMALAKALGWDQELQQLITSERKKLSGSQNSEELQQGKEKPQSDPDKMTSGDRDKKDASL

Target ORF information:

RefSeq Version	NM_142623.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sirtuin 2, transcript variant A (Sirt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142623.4

ORF Insert Sequence:

ATGGATAAGG TTCGACGCTT CTTTGCAAAC ACTCTACATC TCGGCGGCTC CTCGGACGCA 
AAGGAGGAAG TTAAAGTAGA GAAGGTTATT CCAGATTTGA GTTTTGACGG CTTTGCCGAA 
CATTGGCGTG TGCATGGTTT CCGCAAGATT GTGACCATGG TGGGAGCCGG CATATCTACA 
TCTGCTGGCA TTCCGGATTT CAGATCCCCA GGTTCCGGGT TATACAGCAA TCTGAAGAAG 
TACGAGCTGC CACATCCTAC GGCCATATTC GATCTGGACT ACTTCGAAAA GAATCCAGCG 
CCGTTTTTTG CACTGGCCAA GGAACTTTAC CCTGGATCCT TCATTCCCAC GCCGGCCCAC 
TACTTTATCC GTTTGCTTAA CGACAAGGGA CTACTGCAGC GCCACTACAC CCAGAACATA 
GACACGCTAG ACCGGCTTAC TGGCCTGCCC GAGGACAAGA TCATTGAGGC CCATGGCAGC 
TTCCACACCA ACCACTGCAT TAAGTGCCGC AAGGAGTACG ACATGGACTG GATGAAGGCG 
GAGATCTTCG CCGATCGTCT GCCCAAATGC CAAAAGTGCC AAGGCGTTGT TAAACCAGAC 
ATTGTTTTCT TTGGCGAAAA CTTGCCAAAG AGATTTTACT CCAGTCCTGA AGAGGATTTC 
CAGGATTGCG ATCTGCTGAT CATCATGGGC ACATCGTTGG AAGTGCAACC GTTTGCTTCA 
CTGGTGTGGA GACCAGGACC ACGTTGCATT CGCCTCTTGA TCAATCGCGA TGCAGTGGGT 
CAGGCCAGCT GTGTGCTCTT TATGGATCCC AACACGCGAT CGCTACTCTT CGATAAGCCC 
AATAACACTA GGGATGTGGC CTTTCTGGGC GACTGTGATG CTGGCGTAAT GGCTCTGGCC 
AAAGCCTTGG GCTGGGACCA AGAGCTGCAG CAGCTAATTA CAAGTGAAAG GAAGAAACTG 
AGCGGCAGCC AGAATAGTGA GGAGCTGCAA CAAGGCAAAG AGAAACCGCA ATCGGACCCG 
GATAAGATGA CTTCGGGCGA TAGGGACAAG AAGGATGCTT CGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sirt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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