Sb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sb
Entrez Gene ID	41958
Full Name	Stubble
Synonyms	CG4316,Dmel\CG4316,SP56,Sb-sbd,c-SP56,sbd,st-sb
General protein information	Preferred Names Stubble Names CG4316 gene product from transcript CG4316-RB CG4316-PA CG4316-PB Sb-PA Sb-PB Stubble-stubbloid stubble stubbloid
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B4-89B6\|3-58.22 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057361.4	NP_476709.1	stubble, isoform A
NM_001300426.1	NP_001287355.1	stubble, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	CLIPD8	XP_312135.5
Drosophila melanogaster (fruit fly)	Sb	NP_476709.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Helps coordinate proteolytic modification of extracellular matrix attachments with cytoskeletal changes in both legs and bristles.
Title: Mutational analysis of Stubble-stubbloid gene structure and function in Drosophila leg and bristle morphogenesis.

RhoA and Sb-sbd act in a common pathway during leg morphogenesis
Title: Genetic interactions between the RhoA and Stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis.

The following Sb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05089	NM_057361.4 Latest version!	Drosophila melanogaster stubble, transcript variant A (Sb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05089	NM_001300426.1 Latest version!	Drosophila melanogaster stubble, transcript variant B (Sb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sb ( NM_057361.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476709.1 Drosophila melanogaster stubble, transcript variant A (Sb), mRNA.

Price & Availability↑

CloneID	OFa05089
Clone ID Related Accession (Same CDS sequence)	NM_057361.4 , NM_001300426.1
Accession Version	NM_057361.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stubble, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057361.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCAGC CAACTTTAAT CAGACCCAGA CTCAGACACA GAAGAAGCAC ACCAGCGGCA 
GCCACCAAGA TGTGTCCCAA AAGGCATTGG CTAGTCAACA ACAGAGCAGC GGGATCGCGA 
GGATCAGGAG GAGCTGCGGC GCGGAGCAGG CGCAGTCTAG ACCAAATAGT AGAAGTATTA 
GTAGCCTTAA TCGTAGTCAA TTGCCTGGCC ACAGCAGCAG CGGCGCTGAT CACACCGCCC 
GACAGCCTCG AGTCACTGGG CTCCCTGGGA ATCCCGTCAT CTTCCGCCTC CTCCTCCGAG 
GACGACGATG ACATGAGTAG CGGCTTCTAC CGCATCCCGC ACCGCCTGGA GGGCTATCCG 
CAGTTGCAGC AACTGCAGCG CGGCCAGAAC TTCAAGATCA GCCCCAAGCC ATGCTCCTTT 
GGCCGCGTCG AGGGCACCTG CATGTTCGTG TGGGAGTGCA TCAAGTCCGA GGGCAAGCAC 
GTGGGCATGT GCGTCGACTC CTTCATGTTC GGCTCCTGCT GCACGCACAA CTACACCGAC 
AACATTGTCC TGCCCCAGAC GGCCTTCTCC TACACGAGGC CCACCAAGCC GCTCACGCTC 
CGCCCGCGAC CGCCGGCAGC GCCCTACAAG CCGATGATCA GCGGCATGAC CACCATCGAG 
AGGCCTCATG GCGCTGGCAC CCTTGTGATT CGTCCTTCGG GTCCGCACCA CCAGGGCACT 
CTGGCCCGCC CGCATCCGCC GCCCTACCAG AGCAAGCCCA CCACTGCCTC GGATCTGCAT 
GGCTCAGCCT CGCATCCCAG CTCCAGTTCC AGCTCCAGCT CTAGTTCGAA TCCCAATAGC 
ATTTGGCATA CATCGACCCA GCAGCAGCAG CAGCAGCAGC ATCAGCAGAA TCAGCAGAAC 
CACTGGCAGA TGACCACCGA GCCGAGCTTT ATTACCAAGC CGCGACCCAC CGGCTGGACC 
AAGCCCGGCA TCGTCAATTT GCCAATGCCC GCGCGACCCT CCAAGCCGTC GAAGCCCACA 
AAGAAACCGA TTGTCTACGA CCGGACACCG CCACCGCCGC CGTCCGTCCC ACCGTCCACA 
TCCACCTCGA CGACATCAAC GTCGCTGATT TGGCCGGCCC AGACGCATCC CCCGCAGCCG 
CATCGCCCCA CCAGGCCGCA GCTGTCGCCG GGCACATCAT TAGCCGCATC CTCGTCCTCG 
CATTGGCCAT CGTCAACCAC CTCAACCACC TCATCCACCA CATCCACAAC CACAACAACA 
ACAACCACAC GTCGGACAAC CACACCGACG ACAACAACAA GGAGGACCAC GACAAACAAG 
CCCACCAGGC CCTACCAGCG GCCCACCACG GCGACCTCCA GCTCGAGCAC CTCCACCACA 
TCGAGCAAGA CTCCCACCAC GACCAGGCCG ATTAGCTCCA GCAGTTCCAG CAGCAGCGGA 
ATCGTCACCA GCTCCCAACG GCCAACGCAG CCTACGCACC GTACACCGGT TCTGGCCACC 
TCCGGCATCG AGACCAACGA GATATCAGAC TCCTCCATCC CCGACGCCGG AGCTCTGGGC 
CATGTGAAGA CCATTTCGGC GGCTCGCAGC GAGTGCGGAG TACCGACTTT GGCGCGGCCC 
GAGACGCGAA TCGTGGGCGG TAAGAGCGCG GCCTTCGGTC GTTGGCCCTG GCAGGTGTCG 
GTGCGGCGCA CCTCCTTCTT TGGATTCTCG AGCACCCACC GTTGCGGTGG CGCTTTGATC 
AACGAGAACT GGATAGCCAC CGCCGGACAT TGCGTGGACG ACCTGCTCAT CTCGCAGATA 
CGCATCCGCG TGGGCGAGTA CGACTTCTCG CATGTGCAGG AACAGCTGCC CTACATAGAG 
CGCGGGGTGG CCAAGAAGGT GGTGCATCCC AAGTATAGCT TCCTCACGTA CGAATACGAC 
CTGGCGCTGG TCAAGCTGGA GCAGCCGCTC GAATTCGCGC CGCATGTCAG TCCCATTTGC 
CTGCCCGAGA CGGACAGCCT CCTAATTGGC ATGAACGCCA CGGTCACCGG CTGGGGTCGT 
CTCAGCGAGG GCGGCACCCT GCCCTCCGTC CTCCAAGAGG TCTCCGTGCC GATTGTAAGT 
AACGATAATT GCAAATCGAT GTTCATGCGA GCTGGTCGCC AGGAGTTCAT TCCGGATATA 
TTCCTGTGCG CGGGCTATGA AACGGGAGGG CAGGACTCCT GTCAGGGCGA TTCAGGAGGT 
CCACTGCAGG CCAAGTCGCA GGATGGGCGC TTCTTTCTGG CCGGGATCAT CTCCTGGGGC 
ATTGGCTGTG CCGAGGCCAA TTTGCCCGGA GTCTGTACGA GAATCTCCAA GTTTACGCCG 
TGGATACTGG AGCATGTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476709.1
CDS	896..3259
Translation	MKQPTLIRPRLRHRRSTPAAATKMCPKRHWLVNNRAAGSRGSGGAAARSRRSLDQIVEVLVALIVVNCLATAAAALITPPDSLESLGSLGIPSSSASSSEDDDDMSSGFYRIPHRLEGYPQLQQLQRGQNFKISPKPCSFGRVEGTCMFVWECIKSEGKHVGMCVDSFMFGSCCTHNYTDNIVLPQTAFSYTRPTKPLTLRPRPPAAPYKPMISGMTTIERPHGAGTLVIRPSGPHHQGTLARPHPPPYQSKPTTASDLHGSASHPSSSSSSSSSSNPNSIWHTSTQQQQQQQHQQNQQNHWQMTTEPSFITKPRPTGWTKPGIVNLPMPARPSKPSKPTKKPIVYDRTPPPPPSVPPSTSTSTTSTSLIWPAQTHPPQPHRPTRPQLSPGTSLAASSSSHWPSSTTSTTSSTTSTTTTTTTTRRTTTPTTTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSSSSGIVTSSQRPTQPTHRTPVLATSGIETNEISDSSIPDAGALGHVKTISAARSECGVPTLARPETRIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCVDDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANLPGVCTRISKFTPWILEHVR

Target ORF information:

RefSeq Version	NM_057361.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stubble, transcript variant A (Sb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057361.4

ORF Insert Sequence:

ATGAAGCAGC CAACTTTAAT CAGACCCAGA CTCAGACACA GAAGAAGCAC ACCAGCGGCA 
GCCACCAAGA TGTGTCCCAA AAGGCATTGG CTAGTCAACA ACAGAGCAGC GGGATCGCGA 
GGATCAGGAG GAGCTGCGGC GCGGAGCAGG CGCAGTCTAG ACCAAATAGT AGAAGTATTA 
GTAGCCTTAA TCGTAGTCAA TTGCCTGGCC ACAGCAGCAG CGGCGCTGAT CACACCGCCC 
GACAGCCTCG AGTCACTGGG CTCCCTGGGA ATCCCGTCAT CTTCCGCCTC CTCCTCCGAG 
GACGACGATG ACATGAGTAG CGGCTTCTAC CGCATCCCGC ACCGCCTGGA GGGCTATCCG 
CAGTTGCAGC AACTGCAGCG CGGCCAGAAC TTCAAGATCA GCCCCAAGCC ATGCTCCTTT 
GGCCGCGTCG AGGGCACCTG CATGTTCGTG TGGGAGTGCA TCAAGTCCGA GGGCAAGCAC 
GTGGGCATGT GCGTCGACTC CTTCATGTTC GGCTCCTGCT GCACGCACAA CTACACCGAC 
AACATTGTCC TGCCCCAGAC GGCCTTCTCC TACACGAGGC CCACCAAGCC GCTCACGCTC 
CGCCCGCGAC CGCCGGCAGC GCCCTACAAG CCGATGATCA GCGGCATGAC CACCATCGAG 
AGGCCTCATG GCGCTGGCAC CCTTGTGATT CGTCCTTCGG GTCCGCACCA CCAGGGCACT 
CTGGCCCGCC CGCATCCGCC GCCCTACCAG AGCAAGCCCA CCACTGCCTC GGATCTGCAT 
GGCTCAGCCT CGCATCCCAG CTCCAGTTCC AGCTCCAGCT CTAGTTCGAA TCCCAATAGC 
ATTTGGCATA CATCGACCCA GCAGCAGCAG CAGCAGCAGC ATCAGCAGAA TCAGCAGAAC 
CACTGGCAGA TGACCACCGA GCCGAGCTTT ATTACCAAGC CGCGACCCAC CGGCTGGACC 
AAGCCCGGCA TCGTCAATTT GCCAATGCCC GCGCGACCCT CCAAGCCGTC GAAGCCCACA 
AAGAAACCGA TTGTCTACGA CCGGACACCG CCACCGCCGC CGTCCGTCCC ACCGTCCACA 
TCCACCTCGA CGACATCAAC GTCGCTGATT TGGCCGGCCC AGACGCATCC CCCGCAGCCG 
CATCGCCCCA CCAGGCCGCA GCTGTCGCCG GGCACATCAT TAGCCGCATC CTCGTCCTCG 
CATTGGCCAT CGTCAACCAC CTCAACCACC TCATCCACCA CATCCACAAC CACAACAACA 
ACAACCACAC GTCGGACAAC CACACCGACG ACAACAACAA GGAGGACCAC GACAAACAAG 
CCCACCAGGC CCTACCAGCG GCCCACCACG GCGACCTCCA GCTCGAGCAC CTCCACCACA 
TCGAGCAAGA CTCCCACCAC GACCAGGCCG ATTAGCTCCA GCAGTTCCAG CAGCAGCGGA 
ATCGTCACCA GCTCCCAACG GCCAACGCAG CCTACGCACC GTACACCGGT TCTGGCCACC 
TCCGGCATCG AGACCAACGA GATATCAGAC TCCTCCATCC CCGACGCCGG AGCTCTGGGC 
CATGTGAAGA CCATTTCGGC GGCTCGCAGC GAGTGCGGAG TACCGACTTT GGCGCGGCCC 
GAGACGCGAA TCGTGGGCGG TAAGAGCGCG GCCTTCGGTC GTTGGCCCTG GCAGGTGTCG 
GTGCGGCGCA CCTCCTTCTT TGGATTCTCG AGCACCCACC GTTGCGGTGG CGCTTTGATC 
AACGAGAACT GGATAGCCAC CGCCGGACAT TGCGTGGACG ACCTGCTCAT CTCGCAGATA 
CGCATCCGCG TGGGCGAGTA CGACTTCTCG CATGTGCAGG AACAGCTGCC CTACATAGAG 
CGCGGGGTGG CCAAGAAGGT GGTGCATCCC AAGTATAGCT TCCTCACGTA CGAATACGAC 
CTGGCGCTGG TCAAGCTGGA GCAGCCGCTC GAATTCGCGC CGCATGTCAG TCCCATTTGC 
CTGCCCGAGA CGGACAGCCT CCTAATTGGC ATGAACGCCA CGGTCACCGG CTGGGGTCGT 
CTCAGCGAGG GCGGCACCCT GCCCTCCGTC CTCCAAGAGG TCTCCGTGCC GATTGTAAGT 
AACGATAATT GCAAATCGAT GTTCATGCGA GCTGGTCGCC AGGAGTTCAT TCCGGATATA 
TTCCTGTGCG CGGGCTATGA AACGGGAGGG CAGGACTCCT GTCAGGGCGA TTCAGGAGGT 
CCACTGCAGG CCAAGTCGCA GGATGGGCGC TTCTTTCTGG CCGGGATCAT CTCCTGGGGC 
ATTGGCTGTG CCGAGGCCAA TTTGCCCGGA GTCTGTACGA GAATCTCCAA GTTTACGCCG 
TGGATACTGG AGCATGTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sb ( NM_001300426.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287355.1 Drosophila melanogaster stubble, transcript variant B (Sb), mRNA.

Price & Availability↑

CloneID	OFa05089
Clone ID Related Accession (Same CDS sequence)	NM_057361.4 , NM_001300426.1
Accession Version	NM_001300426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stubble, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCAGC CAACTTTAAT CAGACCCAGA CTCAGACACA GAAGAAGCAC ACCAGCGGCA 
GCCACCAAGA TGTGTCCCAA AAGGCATTGG CTAGTCAACA ACAGAGCAGC GGGATCGCGA 
GGATCAGGAG GAGCTGCGGC GCGGAGCAGG CGCAGTCTAG ACCAAATAGT AGAAGTATTA 
GTAGCCTTAA TCGTAGTCAA TTGCCTGGCC ACAGCAGCAG CGGCGCTGAT CACACCGCCC 
GACAGCCTCG AGTCACTGGG CTCCCTGGGA ATCCCGTCAT CTTCCGCCTC CTCCTCCGAG 
GACGACGATG ACATGAGTAG CGGCTTCTAC CGCATCCCGC ACCGCCTGGA GGGCTATCCG 
CAGTTGCAGC AACTGCAGCG CGGCCAGAAC TTCAAGATCA GCCCCAAGCC ATGCTCCTTT 
GGCCGCGTCG AGGGCACCTG CATGTTCGTG TGGGAGTGCA TCAAGTCCGA GGGCAAGCAC 
GTGGGCATGT GCGTCGACTC CTTCATGTTC GGCTCCTGCT GCACGCACAA CTACACCGAC 
AACATTGTCC TGCCCCAGAC GGCCTTCTCC TACACGAGGC CCACCAAGCC GCTCACGCTC 
CGCCCGCGAC CGCCGGCAGC GCCCTACAAG CCGATGATCA GCGGCATGAC CACCATCGAG 
AGGCCTCATG GCGCTGGCAC CCTTGTGATT CGTCCTTCGG GTCCGCACCA CCAGGGCACT 
CTGGCCCGCC CGCATCCGCC GCCCTACCAG AGCAAGCCCA CCACTGCCTC GGATCTGCAT 
GGCTCAGCCT CGCATCCCAG CTCCAGTTCC AGCTCCAGCT CTAGTTCGAA TCCCAATAGC 
ATTTGGCATA CATCGACCCA GCAGCAGCAG CAGCAGCAGC ATCAGCAGAA TCAGCAGAAC 
CACTGGCAGA TGACCACCGA GCCGAGCTTT ATTACCAAGC CGCGACCCAC CGGCTGGACC 
AAGCCCGGCA TCGTCAATTT GCCAATGCCC GCGCGACCCT CCAAGCCGTC GAAGCCCACA 
AAGAAACCGA TTGTCTACGA CCGGACACCG CCACCGCCGC CGTCCGTCCC ACCGTCCACA 
TCCACCTCGA CGACATCAAC GTCGCTGATT TGGCCGGCCC AGACGCATCC CCCGCAGCCG 
CATCGCCCCA CCAGGCCGCA GCTGTCGCCG GGCACATCAT TAGCCGCATC CTCGTCCTCG 
CATTGGCCAT CGTCAACCAC CTCAACCACC TCATCCACCA CATCCACAAC CACAACAACA 
ACAACCACAC GTCGGACAAC CACACCGACG ACAACAACAA GGAGGACCAC GACAAACAAG 
CCCACCAGGC CCTACCAGCG GCCCACCACG GCGACCTCCA GCTCGAGCAC CTCCACCACA 
TCGAGCAAGA CTCCCACCAC GACCAGGCCG ATTAGCTCCA GCAGTTCCAG CAGCAGCGGA 
ATCGTCACCA GCTCCCAACG GCCAACGCAG CCTACGCACC GTACACCGGT TCTGGCCACC 
TCCGGCATCG AGACCAACGA GATATCAGAC TCCTCCATCC CCGACGCCGG AGCTCTGGGC 
CATGTGAAGA CCATTTCGGC GGCTCGCAGC GAGTGCGGAG TACCGACTTT GGCGCGGCCC 
GAGACGCGAA TCGTGGGCGG TAAGAGCGCG GCCTTCGGTC GTTGGCCCTG GCAGGTGTCG 
GTGCGGCGCA CCTCCTTCTT TGGATTCTCG AGCACCCACC GTTGCGGTGG CGCTTTGATC 
AACGAGAACT GGATAGCCAC CGCCGGACAT TGCGTGGACG ACCTGCTCAT CTCGCAGATA 
CGCATCCGCG TGGGCGAGTA CGACTTCTCG CATGTGCAGG AACAGCTGCC CTACATAGAG 
CGCGGGGTGG CCAAGAAGGT GGTGCATCCC AAGTATAGCT TCCTCACGTA CGAATACGAC 
CTGGCGCTGG TCAAGCTGGA GCAGCCGCTC GAATTCGCGC CGCATGTCAG TCCCATTTGC 
CTGCCCGAGA CGGACAGCCT CCTAATTGGC ATGAACGCCA CGGTCACCGG CTGGGGTCGT 
CTCAGCGAGG GCGGCACCCT GCCCTCCGTC CTCCAAGAGG TCTCCGTGCC GATTGTAAGT 
AACGATAATT GCAAATCGAT GTTCATGCGA GCTGGTCGCC AGGAGTTCAT TCCGGATATA 
TTCCTGTGCG CGGGCTATGA AACGGGAGGG CAGGACTCCT GTCAGGGCGA TTCAGGAGGT 
CCACTGCAGG CCAAGTCGCA GGATGGGCGC TTCTTTCTGG CCGGGATCAT CTCCTGGGGC 
ATTGGCTGTG CCGAGGCCAA TTTGCCCGGA GTCTGTACGA GAATCTCCAA GTTTACGCCG 
TGGATACTGG AGCATGTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287355.1
CDS	1140..3503
Translation	MKQPTLIRPRLRHRRSTPAAATKMCPKRHWLVNNRAAGSRGSGGAAARSRRSLDQIVEVLVALIVVNCLATAAAALITPPDSLESLGSLGIPSSSASSSEDDDDMSSGFYRIPHRLEGYPQLQQLQRGQNFKISPKPCSFGRVEGTCMFVWECIKSEGKHVGMCVDSFMFGSCCTHNYTDNIVLPQTAFSYTRPTKPLTLRPRPPAAPYKPMISGMTTIERPHGAGTLVIRPSGPHHQGTLARPHPPPYQSKPTTASDLHGSASHPSSSSSSSSSSNPNSIWHTSTQQQQQQQHQQNQQNHWQMTTEPSFITKPRPTGWTKPGIVNLPMPARPSKPSKPTKKPIVYDRTPPPPPSVPPSTSTSTTSTSLIWPAQTHPPQPHRPTRPQLSPGTSLAASSSSHWPSSTTSTTSSTTSTTTTTTTTRRTTTPTTTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSSSSGIVTSSQRPTQPTHRTPVLATSGIETNEISDSSIPDAGALGHVKTISAARSECGVPTLARPETRIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCVDDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANLPGVCTRISKFTPWILEHVR

Target ORF information:

RefSeq Version	NM_001300426.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stubble, transcript variant B (Sb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300426.1

ORF Insert Sequence:

ATGAAGCAGC CAACTTTAAT CAGACCCAGA CTCAGACACA GAAGAAGCAC ACCAGCGGCA 
GCCACCAAGA TGTGTCCCAA AAGGCATTGG CTAGTCAACA ACAGAGCAGC GGGATCGCGA 
GGATCAGGAG GAGCTGCGGC GCGGAGCAGG CGCAGTCTAG ACCAAATAGT AGAAGTATTA 
GTAGCCTTAA TCGTAGTCAA TTGCCTGGCC ACAGCAGCAG CGGCGCTGAT CACACCGCCC 
GACAGCCTCG AGTCACTGGG CTCCCTGGGA ATCCCGTCAT CTTCCGCCTC CTCCTCCGAG 
GACGACGATG ACATGAGTAG CGGCTTCTAC CGCATCCCGC ACCGCCTGGA GGGCTATCCG 
CAGTTGCAGC AACTGCAGCG CGGCCAGAAC TTCAAGATCA GCCCCAAGCC ATGCTCCTTT 
GGCCGCGTCG AGGGCACCTG CATGTTCGTG TGGGAGTGCA TCAAGTCCGA GGGCAAGCAC 
GTGGGCATGT GCGTCGACTC CTTCATGTTC GGCTCCTGCT GCACGCACAA CTACACCGAC 
AACATTGTCC TGCCCCAGAC GGCCTTCTCC TACACGAGGC CCACCAAGCC GCTCACGCTC 
CGCCCGCGAC CGCCGGCAGC GCCCTACAAG CCGATGATCA GCGGCATGAC CACCATCGAG 
AGGCCTCATG GCGCTGGCAC CCTTGTGATT CGTCCTTCGG GTCCGCACCA CCAGGGCACT 
CTGGCCCGCC CGCATCCGCC GCCCTACCAG AGCAAGCCCA CCACTGCCTC GGATCTGCAT 
GGCTCAGCCT CGCATCCCAG CTCCAGTTCC AGCTCCAGCT CTAGTTCGAA TCCCAATAGC 
ATTTGGCATA CATCGACCCA GCAGCAGCAG CAGCAGCAGC ATCAGCAGAA TCAGCAGAAC 
CACTGGCAGA TGACCACCGA GCCGAGCTTT ATTACCAAGC CGCGACCCAC CGGCTGGACC 
AAGCCCGGCA TCGTCAATTT GCCAATGCCC GCGCGACCCT CCAAGCCGTC GAAGCCCACA 
AAGAAACCGA TTGTCTACGA CCGGACACCG CCACCGCCGC CGTCCGTCCC ACCGTCCACA 
TCCACCTCGA CGACATCAAC GTCGCTGATT TGGCCGGCCC AGACGCATCC CCCGCAGCCG 
CATCGCCCCA CCAGGCCGCA GCTGTCGCCG GGCACATCAT TAGCCGCATC CTCGTCCTCG 
CATTGGCCAT CGTCAACCAC CTCAACCACC TCATCCACCA CATCCACAAC CACAACAACA 
ACAACCACAC GTCGGACAAC CACACCGACG ACAACAACAA GGAGGACCAC GACAAACAAG 
CCCACCAGGC CCTACCAGCG GCCCACCACG GCGACCTCCA GCTCGAGCAC CTCCACCACA 
TCGAGCAAGA CTCCCACCAC GACCAGGCCG ATTAGCTCCA GCAGTTCCAG CAGCAGCGGA 
ATCGTCACCA GCTCCCAACG GCCAACGCAG CCTACGCACC GTACACCGGT TCTGGCCACC 
TCCGGCATCG AGACCAACGA GATATCAGAC TCCTCCATCC CCGACGCCGG AGCTCTGGGC 
CATGTGAAGA CCATTTCGGC GGCTCGCAGC GAGTGCGGAG TACCGACTTT GGCGCGGCCC 
GAGACGCGAA TCGTGGGCGG TAAGAGCGCG GCCTTCGGTC GTTGGCCCTG GCAGGTGTCG 
GTGCGGCGCA CCTCCTTCTT TGGATTCTCG AGCACCCACC GTTGCGGTGG CGCTTTGATC 
AACGAGAACT GGATAGCCAC CGCCGGACAT TGCGTGGACG ACCTGCTCAT CTCGCAGATA 
CGCATCCGCG TGGGCGAGTA CGACTTCTCG CATGTGCAGG AACAGCTGCC CTACATAGAG 
CGCGGGGTGG CCAAGAAGGT GGTGCATCCC AAGTATAGCT TCCTCACGTA CGAATACGAC 
CTGGCGCTGG TCAAGCTGGA GCAGCCGCTC GAATTCGCGC CGCATGTCAG TCCCATTTGC 
CTGCCCGAGA CGGACAGCCT CCTAATTGGC ATGAACGCCA CGGTCACCGG CTGGGGTCGT 
CTCAGCGAGG GCGGCACCCT GCCCTCCGTC CTCCAAGAGG TCTCCGTGCC GATTGTAAGT 
AACGATAATT GCAAATCGAT GTTCATGCGA GCTGGTCGCC AGGAGTTCAT TCCGGATATA 
TTCCTGTGCG CGGGCTATGA AACGGGAGGG CAGGACTCCT GTCAGGGCGA TTCAGGAGGT 
CCACTGCAGG CCAAGTCGCA GGATGGGCGC TTCTTTCTGG CCGGGATCAT CTCCTGGGGC 
ATTGGCTGTG CCGAGGCCAA TTTGCCCGGA GTCTGTACGA GAATCTCCAA GTTTACGCCG 
TGGATACTGG AGCATGTCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sb cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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