MED1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa19226 NM_001275254.1
Latest version!
Drosophila melanogaster mediator complex subunit 1, transcript variant C (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa19227 NM_001202224.2
Latest version!
Drosophila melanogaster mediator complex subunit 1, transcript variant B (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa21195 NM_141084.3
Latest version!
Drosophila melanogaster mediator complex subunit 1, transcript variant A (MED1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa19226
    Clone ID Related Accession (Same CDS sequence) NM_001275254.1
    Accession Version NM_001275254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mediator complex subunit 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGTTTGATGA CGTCGACGGC
    AGCTTTAGCG CCAACAATTC CGGCACCAAC AACAACAGTG GCAGCGGGCT CGGCAGCGTC
    ATTGGTGTCT CCAGTCTCAG CGGTAGTGGG TCAGGGGCAG GAAACAGCGG GAGCAGCGGC
    AGCAGCAACG CTGGCAACAG CAACAATTCT GCAGCAGCAG CCACAGCCGG GAGCAGCGCC
    AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262183.1
    CDS152..3577
    Translation

    Target ORF information:

    RefSeq Version NM_001275254.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mediator complex subunit 1, transcript variant C (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGTTTGATGA CGTCGACGGC
    AGCTTTAGCG CCAACAATTC CGGCACCAAC AACAACAGTG GCAGCGGGCT CGGCAGCGTC
    ATTGGTGTCT CCAGTCTCAG CGGTAGTGGG TCAGGGGCAG GAAACAGCGG GAGCAGCGGC
    AGCAGCAACG CTGGCAACAG CAACAATTCT GCAGCAGCAG CCACAGCCGG GAGCAGCGCC
    AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19227
    Clone ID Related Accession (Same CDS sequence) NM_001202224.2
    Accession Version NM_001202224.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mediator complex subunit 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202224.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTAAGTCC GTTTTCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001189153.1
    CDS152..3394
    Translation

    Target ORF information:

    RefSeq Version NM_001202224.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mediator complex subunit 1, transcript variant B (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001202224.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTAAGTCC GTTTTCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21195
    Clone ID Related Accession (Same CDS sequence) NM_141084.3
    Accession Version NM_141084.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mediator complex subunit 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141084.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTTAGTTT GATGACGTCG
    ACGGCAGCTT TAGCGCCAAC AATTCCGGCA CCAACAACAA CAGTGGCAGC GGGCTCGGCA
    GCGTCATTGG TGTCTCCAGT CTCAGCGGTA GTGGGTCAGG GGCAGGAAAC AGCGGGAGCA
    GCGGCAGCAG CAACGCTGGC AACAGCAACA ATTCTGCAGC AGCAGCCACA GCCGGGAGCA
    GCGCCAACTA GTAGCTGCTT AACCACCAGC GGGGGCAGCA GCGACTCCGC CGGGAGCATC
    AATCCGGCCG GAGCCTCCAC GGAATACATG GTGAAGCCCA GCAGCCAAGA GGGCCTCAAG
    CTGACCATCA ACAAGACGGG AAGCAGCAAG AGCTCCGGCA CCGGCTCCGG CTCTAGTTCC
    TCCTCTGGCC TCCAAGCGAA GGCAAAGAGC AGCAGCAGCG GCGCCACGAG CTTCGCTGGG
    TCCACTGGAT CCACGAAGAA GCAGCATACC GGACTCAAGC CGGGCGTTAA CAGCGGACCC
    GCTTCCAAGA AGGCCACCGC AGCGGTCTCG TCCGCCACGG CATCATCCAG CAAGCATTTC
    TTCCAGAAGG CAAACTCCTC GGGCAATCTC AGCAGCAAGC TCAGTGGGTC GGGATCAGGT
    GGCGGAATTC CGCTGACTAA GAGCAACAGC ACTAACTCCT TCCAAGAGCA CAATGCCCCC
    AGGCGGCGTC CCAGCATGGG AGCCTTGGCT AGCGGCAGCA GTGGAGGAGG CAGCGGGCAG
    CGAAAGTTAG GCTCCGCTTC GGGAGGAGGC AGCGGATCTT CGGGGTCGGT ATCGCCGGCC
    CTTAGCGGCT CAATGTCGCA GCCACCGCCG CGATTCGATC ACCACACGGA TATGATGACC
    ATCCTCCAAT ACGCTTCTCC GACAATGGCC GCAAGCATGG AAGGCTTCAT CAAGGGGCTC
    CACAACAAAT TCCAGATCCC AAAGTTATCG CAGAGAGGAA GCGGAGGCAA TACAACCAGT
    GGACGCAGCA CGCCAAGTGG CAGTTCAGAA CCGGCTTTAG CGGGAACATC ATCTTCGATA
    TTAGGACCTA TTGCCAGCTC TACTGGACTG ACGGAGCCGG AAGCGAAGCC GCCGGTGCCT
    CCTTCGCAGT CTGGCAATGA AGGACTGCTC AATCTTAGCA GCACGGCGGG TACACCATCG
    GCGGATGGGA TAGATGAGGA GCTTTTGGCT AGCCTGGCTG GCGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649341.1
    CDS152..4579
    Translation

    Target ORF information:

    RefSeq Version NM_141084.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mediator complex subunit 1, transcript variant A (MED1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_141084.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
    GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC
    CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG
    AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG
    CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG
    CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG
    TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC
    GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT
    GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC
    AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA
    TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG
    GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG
    CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG
    GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC
    AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT
    CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT
    CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT
    CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA
    ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA
    GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA
    CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT
    CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG
    GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG
    CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC
    ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC
    ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT
    GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC
    GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG
    TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC
    ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT
    ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG
    TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC
    AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA
    AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT
    GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT
    GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT
    ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC
    GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT
    TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC
    TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA
    CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA
    ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT
    GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG
    TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC
    TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA
    GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT
    TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC
    AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT
    ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC
    ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG
    GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT
    TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA
    TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA
    CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTTAGTTT GATGACGTCG
    ACGGCAGCTT TAGCGCCAAC AATTCCGGCA CCAACAACAA CAGTGGCAGC GGGCTCGGCA
    GCGTCATTGG TGTCTCCAGT CTCAGCGGTA GTGGGTCAGG GGCAGGAAAC AGCGGGAGCA
    GCGGCAGCAG CAACGCTGGC AACAGCAACA ATTCTGCAGC AGCAGCCACA GCCGGGAGCA
    GCGCCAACTA GTAGCTGCTT AACCACCAGC GGGGGCAGCA GCGACTCCGC CGGGAGCATC
    AATCCGGCCG GAGCCTCCAC GGAATACATG GTGAAGCCCA GCAGCCAAGA GGGCCTCAAG
    CTGACCATCA ACAAGACGGG AAGCAGCAAG AGCTCCGGCA CCGGCTCCGG CTCTAGTTCC
    TCCTCTGGCC TCCAAGCGAA GGCAAAGAGC AGCAGCAGCG GCGCCACGAG CTTCGCTGGG
    TCCACTGGAT CCACGAAGAA GCAGCATACC GGACTCAAGC CGGGCGTTAA CAGCGGACCC
    GCTTCCAAGA AGGCCACCGC AGCGGTCTCG TCCGCCACGG CATCATCCAG CAAGCATTTC
    TTCCAGAAGG CAAACTCCTC GGGCAATCTC AGCAGCAAGC TCAGTGGGTC GGGATCAGGT
    GGCGGAATTC CGCTGACTAA GAGCAACAGC ACTAACTCCT TCCAAGAGCA CAATGCCCCC
    AGGCGGCGTC CCAGCATGGG AGCCTTGGCT AGCGGCAGCA GTGGAGGAGG CAGCGGGCAG
    CGAAAGTTAG GCTCCGCTTC GGGAGGAGGC AGCGGATCTT CGGGGTCGGT ATCGCCGGCC
    CTTAGCGGCT CAATGTCGCA GCCACCGCCG CGATTCGATC ACCACACGGA TATGATGACC
    ATCCTCCAAT ACGCTTCTCC GACAATGGCC GCAAGCATGG AAGGCTTCAT CAAGGGGCTC
    CACAACAAAT TCCAGATCCC AAAGTTATCG CAGAGAGGAA GCGGAGGCAA TACAACCAGT
    GGACGCAGCA CGCCAAGTGG CAGTTCAGAA CCGGCTTTAG CGGGAACATC ATCTTCGATA
    TTAGGACCTA TTGCCAGCTC TACTGGACTG ACGGAGCCGG AAGCGAAGCC GCCGGTGCCT
    CCTTCGCAGT CTGGCAATGA AGGACTGCTC AATCTTAGCA GCACGGCGGG TACACCATCG
    GCGGATGGGA TAGATGAGGA GCTTTTGGCT AGCCTGGCTG GCGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.