Ssk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ssk
Entrez Gene ID	40207
Full Name	Snakeskin
Synonyms	CG6981,CT21613,Dmel\CG6981,ssk
General protein information	Preferred Names Snakeskin Names CG6981 gene product from transcript CG6981-RB CG6981-PA CG6981-PB Ssk-PA Ssk-PB snakeskin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77A1-77A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140927.5	NP_649184.1	snakeskin, isoform A
NM_168831.3	NP_730487.1	snakeskin, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010471	XP_311473.3
Drosophila melanogaster (fruit fly)	Ssk	NP_730487.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together with Tsp2A, Ssk and Mesh form a protein complex, and all three proteins are required for smooth septate junctions (sSJs) formation, as well as intestinal barrier function.
Title: Molecular dissection of smooth septate junctions: understanding their roles in arthropod physiology.

Thus, a novel protein complex comprising Mesh and Ssk has an important role in sSJ formation and in intestinal barrier function in Drosophila
Title: A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut.

Ssk(CG6981) is required for the intestinal barrier function in Drosophila.
Title: Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila.

The following Ssk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ssk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19064	NM_140927.5 Latest version!	Drosophila melanogaster snakeskin, transcript variant A (Ssk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19064	NM_168831.3 Latest version!	Drosophila melanogaster snakeskin, transcript variant B (Ssk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ssk ( NM_140927.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649184.1 Drosophila melanogaster snakeskin, transcript variant A (Ssk), mRNA.

Price & Availability↑

CloneID	OFa19064
Clone ID Related Accession (Same CDS sequence)	NM_140927.5 , NM_168831.3
Accession Version	NM_140927.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	snakeskin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140927.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCCG TGGAGACTGT AGGCTCTATT TTCATCAAGG CCCTGAAGCT GATCATCAAT 
CTGGTGATCA TCTTCTTGTA CCGTTGGGGC GATGGTGGCG AATTCCTGGG CATCGGCGGC 
ACGTGGAACT TAAACGAGGA GAAGAGCGCC GATGCGGAAA TCGTAGCCTC AGGCGTCATG 
GTTGGATTCT TGATTTACAC TGGATGCCAC ACCATTGCCT TTGCCTTCGG CACAACCAAA 
CATAAGGGAG AGCTGTGCGA CACCATCATG AACGTGGTTG GCTGCATCAT GTGGATCGCC 
GTGGGCGGAG TGGCACTCCA CTACTGGAAG GGTTACATGT CCGACGAGGG ATTCCTGTAT 
GTGAACTCGG AGCGTCAAGT GGGCATTGCC ATGGGCTCGC TGTGCGTTAT CGAGGGCGCA 
CTGTACCTGC TGGACACCGT GTTGGCTTGC ATACACTATT CGAAGGGCGA CACCGACTAC 
ACGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649184.1
CDS	130..618
Translation	MVSVETVGSIFIKALKLIINLVIIFLYRWGDGGEFLGIGGTWNLNEEKSADAEIVASGVMVGFLIYTGCHTIAFAFGTTKHKGELCDTIMNVVGCIMWIAVGGVALHYWKGYMSDEGFLYVNSERQVGIAMGSLCVIEGALYLLDTVLACIHYSKGDTDYTQ

Target ORF information:

RefSeq Version	NM_140927.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster snakeskin, transcript variant A (Ssk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140927.5

ORF Insert Sequence:

ATGGTGTCCG TGGAGACTGT AGGCTCTATT TTCATCAAGG CCCTGAAGCT GATCATCAAT 
CTGGTGATCA TCTTCTTGTA CCGTTGGGGC GATGGTGGCG AATTCCTGGG CATCGGCGGC 
ACGTGGAACT TAAACGAGGA GAAGAGCGCC GATGCGGAAA TCGTAGCCTC AGGCGTCATG 
GTTGGATTCT TGATTTACAC TGGATGCCAC ACCATTGCCT TTGCCTTCGG CACAACCAAA 
CATAAGGGAG AGCTGTGCGA CACCATCATG AACGTGGTTG GCTGCATCAT GTGGATCGCC 
GTGGGCGGAG TGGCACTCCA CTACTGGAAG GGTTACATGT CCGACGAGGG ATTCCTGTAT 
GTGAACTCGG AGCGTCAAGT GGGCATTGCC ATGGGCTCGC TGTGCGTTAT CGAGGGCGCA 
CTGTACCTGC TGGACACCGT GTTGGCTTGC ATACACTATT CGAAGGGCGA CACCGACTAC 
ACGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssk ( NM_168831.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730487.1 Drosophila melanogaster snakeskin, transcript variant B (Ssk), mRNA.

Price & Availability↑

CloneID	OFa19064
Clone ID Related Accession (Same CDS sequence)	NM_140927.5 , NM_168831.3
Accession Version	NM_168831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	snakeskin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168831.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCCG TGGAGACTGT AGGCTCTATT TTCATCAAGG CCCTGAAGCT GATCATCAAT 
CTGGTGATCA TCTTCTTGTA CCGTTGGGGC GATGGTGGCG AATTCCTGGG CATCGGCGGC 
ACGTGGAACT TAAACGAGGA GAAGAGCGCC GATGCGGAAA TCGTAGCCTC AGGCGTCATG 
GTTGGATTCT TGATTTACAC TGGATGCCAC ACCATTGCCT TTGCCTTCGG CACAACCAAA 
CATAAGGGAG AGCTGTGCGA CACCATCATG AACGTGGTTG GCTGCATCAT GTGGATCGCC 
GTGGGCGGAG TGGCACTCCA CTACTGGAAG GGTTACATGT CCGACGAGGG ATTCCTGTAT 
GTGAACTCGG AGCGTCAAGT GGGCATTGCC ATGGGCTCGC TGTGCGTTAT CGAGGGCGCA 
CTGTACCTGC TGGACACCGT GTTGGCTTGC ATACACTATT CGAAGGGCGA CACCGACTAC 
ACGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730487.1
CDS	191..679
Translation	MVSVETVGSIFIKALKLIINLVIIFLYRWGDGGEFLGIGGTWNLNEEKSADAEIVASGVMVGFLIYTGCHTIAFAFGTTKHKGELCDTIMNVVGCIMWIAVGGVALHYWKGYMSDEGFLYVNSERQVGIAMGSLCVIEGALYLLDTVLACIHYSKGDTDYTQ

Target ORF information:

RefSeq Version	NM_168831.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster snakeskin, transcript variant B (Ssk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168831.3

ORF Insert Sequence:

ATGGTGTCCG TGGAGACTGT AGGCTCTATT TTCATCAAGG CCCTGAAGCT GATCATCAAT 
CTGGTGATCA TCTTCTTGTA CCGTTGGGGC GATGGTGGCG AATTCCTGGG CATCGGCGGC 
ACGTGGAACT TAAACGAGGA GAAGAGCGCC GATGCGGAAA TCGTAGCCTC AGGCGTCATG 
GTTGGATTCT TGATTTACAC TGGATGCCAC ACCATTGCCT TTGCCTTCGG CACAACCAAA 
CATAAGGGAG AGCTGTGCGA CACCATCATG AACGTGGTTG GCTGCATCAT GTGGATCGCC 
GTGGGCGGAG TGGCACTCCA CTACTGGAAG GGTTACATGT CCGACGAGGG ATTCCTGTAT 
GTGAACTCGG AGCGTCAAGT GGGCATTGCC ATGGGCTCGC TGTGCGTTAT CGAGGGCGCA 
CTGTACCTGC TGGACACCGT GTTGGCTTGC ATACACTATT CGAAGGGCGA CACCGACTAC 
ACGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ssk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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