Diap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Diap1
Entrez Gene ID	39753
Full Name	Death-associated inhibitor of apoptosis 1
Synonyms	0736/01,1065/03,CG12284,D-IAP1,D-iap1,DIAP,DIAP I,DIAP-1,DIAP1,DIAPI,Diap,Diap-1,Diap-1/Th,Dmel\CG12284,E(rpr)3-1,IAP,IAP1,Iap1,S048915,TH,Thread,dIAP,dIAP-1,dIAP1,dIap1,diap,diap 1,diap-1,diap1,diap1/thread,l(3)72CDe,l(3)72Dc,l(3)S030605,l(3)S030605b,l(3)S106503,l(3)j5C8,th,thread
General protein information	Preferred Names Death-associated inhibitor of apoptosis 1 Names CG12284 gene product from transcript CG12284-RF CG12284-PA CG12284-PB CG12284-PC CG12284-PD CG12284-PE CG12284-PF DIAP1 Diap1-PA Diap1-PB Diap1-PC Diap1-PD Diap1-PE Diap1-PF degradation of inhibitors of apoptosis drosophila IAP1 drosophila inhibitor of apoptosis drosophila inhibitor of apoptosis 1 drosophila inhibitor of apoptosis Protein-1 drosophila inhibitor of apoptosis protein drosophila inhibitor of apoptosis protein 1 drosophila inhibitor-of-apoptosis protein 1 drosophila thread/inhibitor of apoptosis protein-1 inhibitor of apoptosis inhibitor of apoptosis 1 inhibitor of apoptosis protein 1 inhibitor of apoptosis-1 inhibitor of apoptotic protein 1 inhibitors of apoptosis protein lethal (3) 72CDe thread
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72D1-72D5\|3-43.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079377.3	NP_524101.2	Death-associated inhibitor of apoptosis 1, isoform A
NM_168645.2	NP_730098.1	Death-associated inhibitor of apoptosis 1, isoform C
NM_001274988.1	NP_001261917.1	Death-associated inhibitor of apoptosis 1, isoform E
NM_001274989.1	NP_001261918.1	Death-associated inhibitor of apoptosis 1, isoform F
NM_168644.2	NP_730097.1	Death-associated inhibitor of apoptosis 1, isoform B
NM_001274987.1	NP_001261916.1	Death-associated inhibitor of apoptosis 1, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	th	NP_730098.1
Anopheles gambiae (African malaria mosquito)	IAP1	XP_308527.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Accumulation of Mof at the Diap1 promoter 800 bp upstream of the transcription start site in wild type larvae is significantly higher (up to two folds) compared to mof1 mutants.
Title: Drosophila MOF regulates DIAP1 and induces apoptosis in a JNK dependent pathway.

Both germline and somatic stem cells in the adult Drosophila testis are more resistant to apoptosis-inducing stimuli than their differentiating daughters. Niche signaling causes enhanced expression of DIAP1 in both germline stem cells and somatic cyst stem cells, and the resistance to apoptosis is mediated, at least in part, by this enhanced expression.
Title: Niche signaling promotes stem cell survival in the Drosophila testis via the JAK-STAT target DIAP1.

DIAP1 contains distinct instability motifs that regulate inhibitor-of-apoptosis turnover and apoptotic consequences.
Title: Insect inhibitor-of-apoptosis (IAP) proteins are negatively regulated by signal-induced N-terminal degrons absent within viral IAP proteins.

Ubr3 interacts with DIAP1 and positively regulates DIAP1 activity, possibly by maintaining its active conformation in the apoptosis pathway.
Title: Ubr3 E3 ligase regulates apoptosis by controlling the activity of DIAP1 in Drosophila.

epithelial tube size regulation defines new non-apoptotic roles for Yki, DIAP1 and Ice
Title: Non-canonical roles for Yorkie and Drosophila Inhibitor of Apoptosis 1 in epithelial tube size control.

The following Diap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18627	NM_001274987.1 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18627	NM_168645.2 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18627	NM_001274989.1 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18627	NM_168644.2 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18627	NM_079377.3 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18627	NM_001274988.1 Latest version!	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diap1 ( NM_079377.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524101.2 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_079377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079377.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524101.2
CDS	440..1756
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_079377.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079377.3

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap1 ( NM_168645.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730098.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_168645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168645.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730098.1
CDS	242..1558
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_168645.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168645.2

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap1 ( NM_001274988.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261917.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_001274988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261917.1
CDS	726..2042
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_001274988.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274988.1

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap1 ( NM_001274989.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261918.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_001274989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261918.1
CDS	655..1971
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_001274989.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274989.1

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap1 ( NM_168644.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730097.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_168644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168644.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730097.1
CDS	292..1608
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_168644.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168644.2

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diap1 ( NM_001274987.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261916.1 Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18627
Clone ID Related Accession (Same CDS sequence)	NM_079377.3 , NM_168645.2 , NM_001274988.1 , NM_001274989.1 , NM_168644.2 , NM_001274987.1
Accession Version	NM_001274987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Death-associated inhibitor of apoptosis 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261916.1
CDS	176..1492
Translation	MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS

Target ORF information:

RefSeq Version	NM_001274987.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Death-associated inhibitor of apoptosis 1 (Diap1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274987.1

ORF Insert Sequence:

ATGGCATCTG TTGTAGCTGA TCTTCCGTCT TATGGACCTA TCGCTTTTGA TCAGGTGGAT 
AACAACACGA ACGCGACCCA GCTATTCAAA AATAATATAA ACAAAACCAG AATGAACGAT 
TTAAACCGCG AGGAGACGCG ATTAAAGACC TTCACCGACT GGCCGCTGGA CTGGCTGGAT 
AAACGCCAAT TGGCCCAAAC CGGCATGTAC TTCACACACG CCGGCGACAA AGTTAAATGC 
TTTTTCTGCG GCGTGGAAAT CGGTTGCTGG GAGCAGGAGG ATCAGCCCGT GCCGGAACAT 
CAGCGATGGT CGCCCAACTG TCCACTGTTG CGCCGGCGCA CTACCAACAA TGTGCCGATC 
AATGCCGAAG CATTAGATCG CATCCTGCCG CCAATAAGCT ACGATATCTG CGGCGCCAAC 
GACTCGACGC TGGAGATGAG GGAGCATGCC TACGCAGAAG GCGTCATACC CATGTCGCAG 
CTAATTCAGT CGATTGGCAT GAATGCAGTA AATGCGGCAG GCAGTGTAAC TGGAACCGCA 
GCCCCGCAGC CGAGGGTAAC GGTCGCCACC CATGCGTCGA CGGCGACACA GGCCACTGGC 
GATGTCCAGC CGGAGACGTG TCGTCCTTCA GCCGCCAGTG GCAATTATTT TCCCCAGTAT 
CCCGAATACG CCATCGAGAC GGCACGCCTG CGCACCTTCG AGGCTTGGCC GAGGAACCTG 
AAACAGAAGC CCCACCAGCT GGCCGAGGCG GGTTTCTTCT ATACAGGCGT TGGGGATCGC 
GTCCGCTGCT TCAGTTGCGG CGGTGGTCTC ATGGATTGGA ACGACAACGA CGAGCCCTGG 
GAACAGCACG CTCTCTGGCT AAGTCAGTGC CGATTCGTCA AGCTGATGAA GGGTCAGCTC 
TATATCGATA CGGTGGCCGC CAAACCAGTG CTGGCCGAGG AGAAGGAGGA GAGCTCTTCG 
ATTGGAGGGG TCGCGGTGGC CAGCACACAG GCTTCAGAGG AAGAGCAGCA GACATCACTC 
TCATCGGAGG AGGCCGTTTC GGGGGATGTG GCTCCGTCCG TAGCTCCCAC GGCAGCCACA 
CGCATCTTCA ACAAGATCGT CGAGGCGACA GCGGTGGCTA CTCCCTCGAC AAACAGCAGC 
GGCTCCACCT CCATACCCGA GGAAAAGTTG TGCAAGATCT GCTACGGCGC CGAGTACAAT 
ACGGCATTCC TGCCATGCGG TCATGTGGTG GCCTGCGCCA AGTGCGCCTC CTCTGTGACA 
AAGTGTCCGC TGTGCCGGAA GCCCTTCACC GATGTGATGC GCGTATATTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Diap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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