Hsp23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp23
Entrez Gene ID	39077
Full Name	Heat shock protein 23
Synonyms	23,CG4463,DmHSP23,DmHsp23,Dmel20.6,Dmel\CG4463,HSP23,HSP23/IAP,Hsp-23,hsp 22K,hsp 23,hsp 23K,hsp23,hsp23/22,small hsp locus 67B
General protein information	Preferred Names Heat shock protein 23 Names CG4463 gene product from transcript CG4463-RB CG4463-PA CG4463-PB Hsp 23 Hsp23-PA Hsp23-PB heat shock protein 23 heat-shock protein 23 protein 23
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B3-67B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079275.4	NP_523999.1	heat shock protein 23, isoform A
NM_001259765.2	NP_001246694.1	heat shock protein 23, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp23	NP_001246694.1
Drosophila melanogaster (fruit fly)	Hsp27	NP_524000.1
Drosophila melanogaster (fruit fly)	Hsp22	NP_001027114.1
Drosophila melanogaster (fruit fly)	Hsp26	NP_523997.1
Drosophila melanogaster (fruit fly)	Hsp67Ba	NP_523998.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hsp22 and Hsp23 play important roles in the recovery from chill coma in adult males, and suggest that these contribute to adaptive responses to fluctuating thermal conditions.
Title: Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster.

We conclude that histone acetylation stimulates the transcription initiation and promotes the transcription elongation, thereby up-regulating both basal and inducible expression of hsp22 in D. melanogaster.
Title: Histone acetylation regulates both transcription initiation and elongation of hsp22 gene in Drosophila.

The data demonstrate a specific over-expression phenotype for hsp22 and suggest that hsp22 interacts with heat and oxidative stress resistance pathways.
Title: Doxycycline-regulated over-expression of hsp22 has negative effects on stress resistance and life span in adult Drosophila melanogaster.

The following Hsp23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18023	NM_001259765.2 Latest version!	Drosophila melanogaster heat shock protein 23, transcript variant B (Hsp23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18023	NM_079275.4 Latest version!	Drosophila melanogaster heat shock protein 23, transcript variant A (Hsp23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp23 ( NM_079275.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523999.1 Drosophila melanogaster heat shock protein 23, transcript variant A (Hsp23), mRNA.

Price & Availability↑

CloneID	OFa18023
Clone ID Related Accession (Same CDS sequence)	NM_079275.4 , NM_001259765.2
Accession Version	NM_079275.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 23, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079275.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAATA TTCCATTGTT GTTGAGCCTT GCCGACGATT TGGGCCGAAT GTCGATGGTG 
CCCTTCTATG AGCCCTACTA CTGCCAGCGC CAGAGGAATC CCTACTTGGC CCTGGTTGGA 
CCGATGGAGC AGCAGCTGCG CCAGCTGGAG AAACAGGTGG GCGCCTCGTC GGGATCGTCG 
GGAGCCGTGT CGAAAATCGG AAAGGATGGC TTCCAGGTCT GCATGGATGT GTCGCACTTC 
AAGCCCAGCG AACTGGTGGT CAAAGTGCAG GACAACTCCG TCCTGGTGGA GGGCAACCAT 
GAGGAGCGCG AAGATGACCA TGGCTTCATC ACTCGTCACT TTGTCCGCCG CTATGCTCTG 
CCACCCGGTT ATGAGGCTGA TAAGGTGGCC TCCACCTTGT CCTCCGATGG TGTCCTGACC 
ATCAAGGTGC CCAAGCCACC GGCAATCGAG GATAAGGGCA ACGAGCGCAT CGTTCAGATC 
CAGCAGGTGG GACCCGCCCA TCTCAATGTG AAGGAGAATC CCAAGGAGGC GGTGGAGCAG 
GACAATGGCA ACGATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523999.1
CDS	117..677
Translation	MANIPLLLSLADDLGRMSMVPFYEPYYCQRQRNPYLALVGPMEQQLRQLEKQVGASSGSSGAVSKIGKDGFQVCMDVSHFKPSELVVKVQDNSVLVEGNHEEREDDHGFITRHFVRRYALPPGYEADKVASTLSSDGVLTIKVPKPPAIEDKGNERIVQIQQVGPAHLNVKENPKEAVEQDNGNDK

Target ORF information:

RefSeq Version	NM_079275.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 23, transcript variant A (Hsp23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079275.4

ORF Insert Sequence:

ATGGCAAATA TTCCATTGTT GTTGAGCCTT GCCGACGATT TGGGCCGAAT GTCGATGGTG 
CCCTTCTATG AGCCCTACTA CTGCCAGCGC CAGAGGAATC CCTACTTGGC CCTGGTTGGA 
CCGATGGAGC AGCAGCTGCG CCAGCTGGAG AAACAGGTGG GCGCCTCGTC GGGATCGTCG 
GGAGCCGTGT CGAAAATCGG AAAGGATGGC TTCCAGGTCT GCATGGATGT GTCGCACTTC 
AAGCCCAGCG AACTGGTGGT CAAAGTGCAG GACAACTCCG TCCTGGTGGA GGGCAACCAT 
GAGGAGCGCG AAGATGACCA TGGCTTCATC ACTCGTCACT TTGTCCGCCG CTATGCTCTG 
CCACCCGGTT ATGAGGCTGA TAAGGTGGCC TCCACCTTGT CCTCCGATGG TGTCCTGACC 
ATCAAGGTGC CCAAGCCACC GGCAATCGAG GATAAGGGCA ACGAGCGCAT CGTTCAGATC 
CAGCAGGTGG GACCCGCCCA TCTCAATGTG AAGGAGAATC CCAAGGAGGC GGTGGAGCAG 
GACAATGGCA ACGATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsp23 ( NM_001259765.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246694.1 Drosophila melanogaster heat shock protein 23, transcript variant B (Hsp23), mRNA.

Price & Availability↑

CloneID	OFa18023
Clone ID Related Accession (Same CDS sequence)	NM_079275.4 , NM_001259765.2
Accession Version	NM_001259765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 23, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259765.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAATA TTCCATTGTT GTTGAGCCTT GCCGACGATT TGGGCCGAAT GTCGATGGTG 
CCCTTCTATG AGCCCTACTA CTGCCAGCGC CAGAGGAATC CCTACTTGGC CCTGGTTGGA 
CCGATGGAGC AGCAGCTGCG CCAGCTGGAG AAACAGGTGG GCGCCTCGTC GGGATCGTCG 
GGAGCCGTGT CGAAAATCGG AAAGGATGGC TTCCAGGTCT GCATGGATGT GTCGCACTTC 
AAGCCCAGCG AACTGGTGGT CAAAGTGCAG GACAACTCCG TCCTGGTGGA GGGCAACCAT 
GAGGAGCGCG AAGATGACCA TGGCTTCATC ACTCGTCACT TTGTCCGCCG CTATGCTCTG 
CCACCCGGTT ATGAGGCTGA TAAGGTGGCC TCCACCTTGT CCTCCGATGG TGTCCTGACC 
ATCAAGGTGC CCAAGCCACC GGCAATCGAG GATAAGGGCA ACGAGCGCAT CGTTCAGATC 
CAGCAGGTGG GACCCGCCCA TCTCAATGTG AAGGAGAATC CCAAGGAGGC GGTGGAGCAG 
GACAATGGCA ACGATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246694.1
CDS	117..677
Translation	MANIPLLLSLADDLGRMSMVPFYEPYYCQRQRNPYLALVGPMEQQLRQLEKQVGASSGSSGAVSKIGKDGFQVCMDVSHFKPSELVVKVQDNSVLVEGNHEEREDDHGFITRHFVRRYALPPGYEADKVASTLSSDGVLTIKVPKPPAIEDKGNERIVQIQQVGPAHLNVKENPKEAVEQDNGNDK

Target ORF information:

RefSeq Version	NM_001259765.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 23, transcript variant B (Hsp23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259765.2

ORF Insert Sequence:

ATGGCAAATA TTCCATTGTT GTTGAGCCTT GCCGACGATT TGGGCCGAAT GTCGATGGTG 
CCCTTCTATG AGCCCTACTA CTGCCAGCGC CAGAGGAATC CCTACTTGGC CCTGGTTGGA 
CCGATGGAGC AGCAGCTGCG CCAGCTGGAG AAACAGGTGG GCGCCTCGTC GGGATCGTCG 
GGAGCCGTGT CGAAAATCGG AAAGGATGGC TTCCAGGTCT GCATGGATGT GTCGCACTTC 
AAGCCCAGCG AACTGGTGGT CAAAGTGCAG GACAACTCCG TCCTGGTGGA GGGCAACCAT 
GAGGAGCGCG AAGATGACCA TGGCTTCATC ACTCGTCACT TTGTCCGCCG CTATGCTCTG 
CCACCCGGTT ATGAGGCTGA TAAGGTGGCC TCCACCTTGT CCTCCGATGG TGTCCTGACC 
ATCAAGGTGC CCAAGCCACC GGCAATCGAG GATAAGGGCA ACGAGCGCAT CGTTCAGATC 
CAGCAGGTGG GACCCGCCCA TCTCAATGTG AAGGAGAATC CCAAGGAGGC GGTGGAGCAG 
GACAATGGCA ACGATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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