Hsp22 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp22
Entrez Gene ID	3772576
Full Name	Heat shock protein 22
Synonyms	22,CG32041,CG4460,DmHsp22,Dmel20.8,Dmel\CG4460,EP(3)3247,HSP22,anon-WO0118547.50,anon-WO0140519.138,hsp22,small hsp locus 67B
General protein information	Preferred Names Heat shock protein 22 Names CG4460 gene product from transcript CG4460-RA CG4460-PA CG4460-PB Hsp22-PA Hsp22-PB heat shock protein 22 heat shock protein hsp22 heat-shock protein 22 protein 22
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B2-67B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031943.3	NP_001027114.1	heat shock protein 22, isoform B
NM_001031944.2	NP_001027115.1	heat shock protein 22, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp23	NP_001246694.1
Drosophila melanogaster (fruit fly)	Hsp27	NP_524000.1
Drosophila melanogaster (fruit fly)	Hsp22	NP_001027114.1
Drosophila melanogaster (fruit fly)	Hsp26	NP_523997.1
Drosophila melanogaster (fruit fly)	Hsp67Ba	NP_523998.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together the results are consistent with a role of Hsp22 in the UPR(MT) and in mitochondrial proteostasis.
Title: Changes in Drosophila mitochondrial proteins following chaperone-mediated lifespan extension confirm a role of Hsp22 in mitochondrial UPR and reveal a mitochondrial localization for cathepsin D.

hsp22 could play an important role in relation to stress resistance and adaptation.
Title: Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants.

Hsp22 and Hsp23 play important roles in the recovery from chill coma in adult males, and suggest that these contribute to adaptive responses to fluctuating thermal conditions.
Title: Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster.

The up-regulation of genes of the OXPHOS system in Hsp22+ flies suggest that mitochondrial homeostasis is at the center of Hsp22 beneficial effects on lifespan.
Title: Gene expression profiling implicates OXPHOS complexes in lifespan extension of flies over-expressing a small mitochondrial chaperone, Hsp22.

Data show that the DmHsp22 interacts and inactivates wild type tumor suppressor protein p53, which may be one possible way of its functioning in human cells.
Title: Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells.

The following Hsp22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21302	NM_001031944.2 Latest version!	Drosophila melanogaster heat shock protein 22, transcript variant A (Hsp22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa21302	NM_001031943.3 Latest version!	Drosophila melanogaster heat shock protein 22, transcript variant B (Hsp22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp22 ( NM_001031943.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027114.1 Drosophila melanogaster heat shock protein 22, transcript variant B (Hsp22), mRNA.

Price & Availability↑

CloneID	OFa21302
Clone ID Related Accession (Same CDS sequence)	NM_001031943.3 , NM_001031944.2
Accession Version	NM_001031943.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 22, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001031943.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTTCCT TACCGATGTT TTGGCGCATG GCCGAGGAGA TGGCACGGAT GCCACGCCTC 
TCCTCGCCCT TTCACGCCTT CTTCCACGAG CCGCCCGTTT GGAGTGTGGC GCTACCGAGG 
AACTGGCAGC AGATTGCCCG CTGGCAGGAG CAGGAGTTCG CTCCGCCGGC CACCGTCAAC 
AAGGATGGCT ACAAACTCAC CCTGGACGTC AAGGACTACA GCGAGCTAAA GGTCAAGGTG 
CTGGACGAGA GCGTTGTCCT GGTGGAGGGA AAATCAGAGC AGCAGGAGGC CGAACAAGGT 
GGCTATAGCT CCAGGCACTT CCTCCGCCGC TTCGTCCTGC CGGAAGGATA CGAGGCGGAC 
AAGGTGACCT CAACGCTGAG CAGCGACGGC GTCCTGACCA TCAGTGTGCC CAATCCTCCA 
GGCGTGCAGG AGACACTCAA GGAGCGTGAG GTGACCATCG AGCAGACTGG CGAGCCGGCA 
AAGAAGTCCG CCGAGGAGCC AAATGACAAA GCCGCCAGTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027114.1
CDS	255..779
Translation	MRSLPMFWRMAEEMARMPRLSSPFHAFFHEPPVWSVALPRNWQQIARWQEQEFAPPATVNKDGYKLTLDVKDYSELKVKVLDESVVLVEGKSEQQEAEQGGYSSRHFLRRFVLPEGYEADKVTSTLSSDGVLTISVPNPPGVQETLKEREVTIEQTGEPAKKSAEEPNDKAASQ

Target ORF information:

RefSeq Version	NM_001031943.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 22, transcript variant B (Hsp22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031943.3

ORF Insert Sequence:

ATGCGTTCCT TACCGATGTT TTGGCGCATG GCCGAGGAGA TGGCACGGAT GCCACGCCTC 
TCCTCGCCCT TTCACGCCTT CTTCCACGAG CCGCCCGTTT GGAGTGTGGC GCTACCGAGG 
AACTGGCAGC AGATTGCCCG CTGGCAGGAG CAGGAGTTCG CTCCGCCGGC CACCGTCAAC 
AAGGATGGCT ACAAACTCAC CCTGGACGTC AAGGACTACA GCGAGCTAAA GGTCAAGGTG 
CTGGACGAGA GCGTTGTCCT GGTGGAGGGA AAATCAGAGC AGCAGGAGGC CGAACAAGGT 
GGCTATAGCT CCAGGCACTT CCTCCGCCGC TTCGTCCTGC CGGAAGGATA CGAGGCGGAC 
AAGGTGACCT CAACGCTGAG CAGCGACGGC GTCCTGACCA TCAGTGTGCC CAATCCTCCA 
GGCGTGCAGG AGACACTCAA GGAGCGTGAG GTGACCATCG AGCAGACTGG CGAGCCGGCA 
AAGAAGTCCG CCGAGGAGCC AAATGACAAA GCCGCCAGTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsp22 ( NM_001031944.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027115.1 Drosophila melanogaster heat shock protein 22, transcript variant A (Hsp22), mRNA.

Price & Availability↑

CloneID	OFa21302
Clone ID Related Accession (Same CDS sequence)	NM_001031943.3 , NM_001031944.2
Accession Version	NM_001031944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 22, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_001031944.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTTCCT TACCGATGTT TTGGCGCATG GCCGAGGAGA TGGCACGGAT GCCACGCCTC 
TCCTCGCCCT TTCACGCCTT CTTCCACGAG CCGCCCGTTT GGAGTGTGGC GCTACCGAGG 
AACTGGCAGC AGATTGCCCG CTGGCAGGAG CAGGAGTTCG CTCCGCCGGC CACCGTCAAC 
AAGGATGGCT ACAAACTCAC CCTGGACGTC AAGGACTACA GCGAGCTAAA GGTCAAGGTG 
CTGGACGAGA GCGTTGTCCT GGTGGAGGGA AAATCAGAGC AGCAGGAGGC CGAACAAGGT 
GGCTATAGCT CCAGGCACTT CCTCCGCCGC TTCGTCCTGC CGGAAGGATA CGAGGCGGAC 
AAGGTGACCT CAACGCTGAG CAGCGACGGC GTCCTGACCA TCAGTGTGCC CAATCCTCCA 
GGCGTGCAGG AGACACTCAA GGAGCGTGAG GTGACCATCG AGCAGACTGG CGAGCCGGCA 
AAGAAGTCCG CCGAGGAGCC AAATGACAAA GCCGCCAGTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027115.1
CDS	1187..1711
Translation	MRSLPMFWRMAEEMARMPRLSSPFHAFFHEPPVWSVALPRNWQQIARWQEQEFAPPATVNKDGYKLTLDVKDYSELKVKVLDESVVLVEGKSEQQEAEQGGYSSRHFLRRFVLPEGYEADKVTSTLSSDGVLTISVPNPPGVQETLKEREVTIEQTGEPAKKSAEEPNDKAASQ

Target ORF information:

RefSeq Version	NM_001031944.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 22, transcript variant A (Hsp22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031944.2

ORF Insert Sequence:

ATGCGTTCCT TACCGATGTT TTGGCGCATG GCCGAGGAGA TGGCACGGAT GCCACGCCTC 
TCCTCGCCCT TTCACGCCTT CTTCCACGAG CCGCCCGTTT GGAGTGTGGC GCTACCGAGG 
AACTGGCAGC AGATTGCCCG CTGGCAGGAG CAGGAGTTCG CTCCGCCGGC CACCGTCAAC 
AAGGATGGCT ACAAACTCAC CCTGGACGTC AAGGACTACA GCGAGCTAAA GGTCAAGGTG 
CTGGACGAGA GCGTTGTCCT GGTGGAGGGA AAATCAGAGC AGCAGGAGGC CGAACAAGGT 
GGCTATAGCT CCAGGCACTT CCTCCGCCGC TTCGTCCTGC CGGAAGGATA CGAGGCGGAC 
AAGGTGACCT CAACGCTGAG CAGCGACGGC GTCCTGACCA TCAGTGTGCC CAATCCTCCA 
GGCGTGCAGG AGACACTCAA GGAGCGTGAG GTGACCATCG AGCAGACTGG CGAGCCGGCA 
AAGAAGTCCG CCGAGGAGCC AAATGACAAA GCCGCCAGTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp22 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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