bs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bs
Entrez Gene ID	37890
Full Name	blistered
Synonyms	BS,Bs,CG3411,D-SRF,DSRF,DSrf,DSrf/bs,DmSRF,Dmel\CG3411,Group IIc,Mal,SRF,Serf,Srf,ba,bs/DSRF,bs/Dsrf,dSRF,dsrf,l(2)03267,pruned/DSRF,px,srf
General protein information	Preferred Names blistered Names CG3411 gene product from transcript CG3411-RC CG3411-PA CG3411-PB CG3411-PC SRF balloon blistered/DSRF blistered/Drosophila serum response factor bs-PA bs-PB bs-PC drosophila serum response factor pruned serum response factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C6-60C7\|2-107.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299908.1	NP_001286837.1	blistered, isoform C
NM_001144279.3	NP_001137751.1	blistered, isoform B
NM_166667.3	NP_726438.1	blistered, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that DSRF acts as an amplifying step to sustain the progression of terminal branch elongation of the trachael system
Title: The Drosophila homologue of SRF acts as a boosting mechanism to sustain FGF-induced terminal branching in the tracheal system.

Data describe a myocardin-related transcription factor from Drosophila, DMRTF, which is shares homology with mammalian myocardin and MRTFs and stimulates the activity of Drosophila serum response factor.
Title: A myocardin-related transcription factor regulates activity of serum response factor in Drosophila.

Border cell migration requires the activity of serum response factor and nuclear accumulation of MAL-D is induced by cell stretching.
Title: Evidence for tension-based regulation of Drosophila MAL and SRF during invasive cell migration.

The following bs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11157	NM_001299908.1 Latest version!	Drosophila melanogaster blistered (bs), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa11157	NM_166667.3 Latest version!	Drosophila melanogaster blistered (bs), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa13226	NM_001144279.3 Latest version!	Drosophila melanogaster blistered (bs), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bs ( NM_001299908.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286837.1 Drosophila melanogaster blistered (bs), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11157
Clone ID Related Accession (Same CDS sequence)	NM_001299908.1 , NM_166667.3
Accession Version	NM_001299908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistered, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGATGAC 
CGATCGCCTA CCTCGTCGGG CAACGAGTCG GATGATTCGT CGGACGTGGA GATGCCGGCG 
GAGGCTGCTG AGGTGGCAAC CAAGTTGCCG GCCAGCAAAA CAGAGGTATC AGCGCCTCCA 
GCAGCCTCAT GCTCGGCGGC CACCGCTTCC TCTGGCCACA AAACCATGCC AGCATTGAAC 
TATCAGACTG ACACCAACAG CGGACCCTCA ACGTCCACCG CCGCCGGAGG AGGAGGCTCG 
GCGGACAGCA AGTACGTCTA CTCCGCTGCC TCGATTGCCA ACATACCGCA GAAGATGCTG 
AGGCAGCTCA TCCAGTCAGG CCACTTGCAG GTGCACGCCG AAGAGGATGG CAACCAGTAT 
GTGACCATAC CGCTCAGCTC CACAGCAGCC AATCTGATAA AGAGCAATAA GCTAACAGCC 
TCTGGATCAG GAGCCTCAGG ATCGGGAACG CCCGTCAAGA ACGATGCCAG TGCCGATAAG 
CCCTTGACCA TCAAACAGGA GTTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286837.1
CDS	1230..2579
Translation	MDPTRGDSRYNLNYSMSSIGLSLADPADMYGNPALGAGRPPSGGLLQNMGGVPPMQRPNPAAGAPPAPQCQTLHSPQHASQQQQQQQQQQQQHQQQQQQQQQHPQQQRGLKRSGSDCYEDHHRSSGGLTLQGLDNVASGAVDDSVDNYNPLPNKKSPPANGKKTKGRVKIKMEYIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVMLLVASETGHVYTFATRKLQPMITSEAGKQLIQTCLNSPDPPSVGGGDQRMSATGFEETELSYNIADEDSKDDRSPTSSGNESDDSSDVEMPAEAAEVATKLPASKTEVSAPPAASCSAATASSGHKTMPALNYQTDTNSGPSTSTAAGGGGSADSKYVYSAASIANIPQKMLRQLIQSGHLQVHAEEDGNQYVTIPLSSTAANLIKSNKLTASGSGASGSGTPVKNDASADKPLTIKQEFD

Target ORF information:

RefSeq Version	NM_001299908.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistered (bs), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299908.1

ORF Insert Sequence:

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGATGAC 
CGATCGCCTA CCTCGTCGGG CAACGAGTCG GATGATTCGT CGGACGTGGA GATGCCGGCG 
GAGGCTGCTG AGGTGGCAAC CAAGTTGCCG GCCAGCAAAA CAGAGGTATC AGCGCCTCCA 
GCAGCCTCAT GCTCGGCGGC CACCGCTTCC TCTGGCCACA AAACCATGCC AGCATTGAAC 
TATCAGACTG ACACCAACAG CGGACCCTCA ACGTCCACCG CCGCCGGAGG AGGAGGCTCG 
GCGGACAGCA AGTACGTCTA CTCCGCTGCC TCGATTGCCA ACATACCGCA GAAGATGCTG 
AGGCAGCTCA TCCAGTCAGG CCACTTGCAG GTGCACGCCG AAGAGGATGG CAACCAGTAT 
GTGACCATAC CGCTCAGCTC CACAGCAGCC AATCTGATAA AGAGCAATAA GCTAACAGCC 
TCTGGATCAG GAGCCTCAGG ATCGGGAACG CCCGTCAAGA ACGATGCCAG TGCCGATAAG 
CCCTTGACCA TCAAACAGGA GTTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bs ( NM_166667.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726438.1 Drosophila melanogaster blistered (bs), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11157
Clone ID Related Accession (Same CDS sequence)	NM_001299908.1 , NM_166667.3
Accession Version	NM_166667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistered, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166667.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGATGAC 
CGATCGCCTA CCTCGTCGGG CAACGAGTCG GATGATTCGT CGGACGTGGA GATGCCGGCG 
GAGGCTGCTG AGGTGGCAAC CAAGTTGCCG GCCAGCAAAA CAGAGGTATC AGCGCCTCCA 
GCAGCCTCAT GCTCGGCGGC CACCGCTTCC TCTGGCCACA AAACCATGCC AGCATTGAAC 
TATCAGACTG ACACCAACAG CGGACCCTCA ACGTCCACCG CCGCCGGAGG AGGAGGCTCG 
GCGGACAGCA AGTACGTCTA CTCCGCTGCC TCGATTGCCA ACATACCGCA GAAGATGCTG 
AGGCAGCTCA TCCAGTCAGG CCACTTGCAG GTGCACGCCG AAGAGGATGG CAACCAGTAT 
GTGACCATAC CGCTCAGCTC CACAGCAGCC AATCTGATAA AGAGCAATAA GCTAACAGCC 
TCTGGATCAG GAGCCTCAGG ATCGGGAACG CCCGTCAAGA ACGATGCCAG TGCCGATAAG 
CCCTTGACCA TCAAACAGGA GTTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726438.1
CDS	389..1738
Translation	MDPTRGDSRYNLNYSMSSIGLSLADPADMYGNPALGAGRPPSGGLLQNMGGVPPMQRPNPAAGAPPAPQCQTLHSPQHASQQQQQQQQQQQQHQQQQQQQQQHPQQQRGLKRSGSDCYEDHHRSSGGLTLQGLDNVASGAVDDSVDNYNPLPNKKSPPANGKKTKGRVKIKMEYIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVMLLVASETGHVYTFATRKLQPMITSEAGKQLIQTCLNSPDPPSVGGGDQRMSATGFEETELSYNIADEDSKDDRSPTSSGNESDDSSDVEMPAEAAEVATKLPASKTEVSAPPAASCSAATASSGHKTMPALNYQTDTNSGPSTSTAAGGGGSADSKYVYSAASIANIPQKMLRQLIQSGHLQVHAEEDGNQYVTIPLSSTAANLIKSNKLTASGSGASGSGTPVKNDASADKPLTIKQEFD

Target ORF information:

RefSeq Version	NM_166667.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistered (bs), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166667.3

ORF Insert Sequence:

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGATGAC 
CGATCGCCTA CCTCGTCGGG CAACGAGTCG GATGATTCGT CGGACGTGGA GATGCCGGCG 
GAGGCTGCTG AGGTGGCAAC CAAGTTGCCG GCCAGCAAAA CAGAGGTATC AGCGCCTCCA 
GCAGCCTCAT GCTCGGCGGC CACCGCTTCC TCTGGCCACA AAACCATGCC AGCATTGAAC 
TATCAGACTG ACACCAACAG CGGACCCTCA ACGTCCACCG CCGCCGGAGG AGGAGGCTCG 
GCGGACAGCA AGTACGTCTA CTCCGCTGCC TCGATTGCCA ACATACCGCA GAAGATGCTG 
AGGCAGCTCA TCCAGTCAGG CCACTTGCAG GTGCACGCCG AAGAGGATGG CAACCAGTAT 
GTGACCATAC CGCTCAGCTC CACAGCAGCC AATCTGATAA AGAGCAATAA GCTAACAGCC 
TCTGGATCAG GAGCCTCAGG ATCGGGAACG CCCGTCAAGA ACGATGCCAG TGCCGATAAG 
CCCTTGACCA TCAAACAGGA GTTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bs ( NM_001144279.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137751.1 Drosophila melanogaster blistered (bs), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13226
Clone ID Related Accession (Same CDS sequence)	NM_001144279.3
Accession Version	NM_001144279.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistered, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144279.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGTAAGA 
CAATTGATAT ACGGTCACCA CGGGCACGCT CATCTAGGTC ACCCGCCGGC GCCGCCCTCG 
CACTACAGCC TCGAGCAGAG CCTGATGCAG TCGGGCTACA GCGGCGGCCA GGCCTCGCCG 
CTTTCCCACG CCCAGTACGG CGGACACGGG CACCACGCCC ACGTGCCGCA CGTGCCGCCG 
CCGCATTCGA CGCACTCGCA CATGTCCCAC ACGCATGCGC AGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137751.1
CDS	1230..2297
Translation	MDPTRGDSRYNLNYSMSSIGLSLADPADMYGNPALGAGRPPSGGLLQNMGGVPPMQRPNPAAGAPPAPQCQTLHSPQHASQQQQQQQQQQQQHQQQQQQQQQHPQQQRGLKRSGSDCYEDHHRSSGGLTLQGLDNVASGAVDDSVDNYNPLPNKKSPPANGKKTKGRVKIKMEYIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVMLLVASETGHVYTFATRKLQPMITSEAGKQLIQTCLNSPDPPSVGGGDQRMSATGFEETELSYNIADEDSKVRQLIYGHHGHAHLGHPPAPPSHYSLEQSLMQSGYSGGQASPLSHAQYGGHGHHAHVPHVPPPHSTHSHMSHTHAQR

Target ORF information:

RefSeq Version	NM_001144279.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistered (bs), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144279.3

ORF Insert Sequence:

ATGGATCCGA CGCGAGGTGA CTCGCGGTAC AATCTCAACT ACTCGATGAG TAGCATAGGG 
CTGAGCCTGG CGGATCCGGC GGACATGTAC GGCAACCCGG CCCTGGGGGC GGGCCGCCCG 
CCCTCCGGTG GCCTGCTCCA GAACATGGGC GGCGTTCCGC CGATGCAGCG CCCCAATCCC 
GCGGCAGGAG CACCCCCTGC CCCGCAGTGC CAAACGCTCC ACAGCCCACA GCACGCCTCC 
CAGCAGCAGC AGCAGCAACA ACAGCAGCAG CAGCAACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGCATC CCCAGCAGCA ACGCGGCCTT AAGCGCTCCG GCAGCGACTG CTACGAGGAT 
CACCACCGCT CGTCCGGCGG ACTCACCCTC CAAGGACTGG ACAACGTGGC CAGCGGAGCC 
GTCGACGACA GCGTAGACAA CTACAACCCG CTGCCCAACA AGAAGTCTCC GCCTGCCAAC 
GGCAAAAAGA CAAAGGGACG TGTCAAAATC AAAATGGAAT ACATCGACAA TAAGCTGCGT 
CGCTACACGA CCTTCTCCAA GCGCAAGACG GGCATAATGA AGAAGGCCTA CGAGCTGTCC 
ACGCTGACCG GGACGCAGGT GATGCTGCTG GTGGCCAGCG AGACGGGCCA CGTGTACACA 
TTTGCCACGC GCAAGCTGCA GCCGATGATC ACCTCGGAGG CGGGAAAGCA GCTCATCCAG 
ACCTGCCTCA ACTCGCCGGA TCCGCCCAGT GTGGGCGGCG GCGACCAACG GATGTCGGCC 
ACCGGGTTCG AGGAGACGGA GCTCAGCTAC AACATCGCCG ACGAGGACTC GAAAGTAAGA 
CAATTGATAT ACGGTCACCA CGGGCACGCT CATCTAGGTC ACCCGCCGGC GCCGCCCTCG 
CACTACAGCC TCGAGCAGAG CCTGATGCAG TCGGGCTACA GCGGCGGCCA GGCCTCGCCG 
CTTTCCCACG CCCAGTACGG CGGACACGGG CACCACGCCC ACGTGCCGCA CGTGCCGCCG 
CCGCATTCGA CGCACTCGCA CATGTCCCAC ACGCATGCGC AGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bs cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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