RhoGEF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following RhoGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa10304 NM_206146.4
Latest version!
Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa10304 NM_057969.6
Latest version!
Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa10305 NM_206147.4
Latest version!
Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa12368 NM_001259452.2
Latest version!
Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa21838 NM_001299586.1
Latest version!
Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa10304
    Clone ID Related Accession (Same CDS sequence) NM_206146.4 , NM_057969.6
    Accession Version NM_206146.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Rho guanine nucleotide exchange factor 2, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206146.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC
    AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG
    AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT
    GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC
    TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA
    GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG
    CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA
    GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG
    CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG
    AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG
    GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG
    GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG
    TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT
    CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC
    GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC
    CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA
    AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG
    CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG
    GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG
    GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT
    CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT
    AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG
    AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG
    CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA
    GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA
    AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT
    AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG
    AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC
    GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA
    TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG
    GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG
    CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA
    AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA
    CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT
    CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT
    GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG
    AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC
    AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC
    TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC
    CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG
    AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG
    CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT
    TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT
    TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG
    ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA
    CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC
    AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA
    CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG
    GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA
    ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG
    GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG
    GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA
    GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT
    AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA
    TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG
    CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG
    CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT
    CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG
    TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC
    ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC
    AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995868.1
    CDS474..8153
    Translation

    Target ORF information:

    RefSeq Version NM_206146.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206146.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC
    AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG
    AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT
    GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC
    TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA
    GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG
    CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA
    GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG
    CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG
    AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG
    GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG
    GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG
    TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT
    CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC
    GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC
    CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA
    AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG
    CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG
    GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG
    GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT
    CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT
    AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG
    AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG
    CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA
    GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA
    AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT
    AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG
    AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC
    GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA
    TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG
    GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG
    CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA
    AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA
    CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT
    CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT
    GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG
    AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC
    AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC
    TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC
    CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG
    AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG
    CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT
    TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT
    TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG
    ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA
    CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC
    AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA
    CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG
    GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA
    ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG
    GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG
    GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA
    GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT
    AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA
    TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG
    CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG
    CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT
    CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG
    TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC
    ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC
    AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa10304
    Clone ID Related Accession (Same CDS sequence) NM_206146.4 , NM_057969.6
    Accession Version NM_057969.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Rho guanine nucleotide exchange factor 2, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057969.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC
    AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG
    AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT
    GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC
    TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA
    GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG
    CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA
    GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG
    CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG
    AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG
    GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG
    GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG
    TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT
    CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC
    GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC
    CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA
    AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG
    CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG
    GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG
    GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT
    CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT
    AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG
    AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG
    CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA
    GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA
    AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT
    AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG
    AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC
    GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA
    TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG
    GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG
    CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA
    AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA
    CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT
    CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT
    GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG
    AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC
    AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC
    TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC
    CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG
    AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG
    CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT
    TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT
    TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG
    ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA
    CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC
    AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA
    CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG
    GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA
    ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG
    GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG
    GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA
    GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT
    AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA
    TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG
    CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG
    CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT
    CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG
    TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC
    ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC
    AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477317.1
    CDS463..8142
    Translation

    Target ORF information:

    RefSeq Version NM_057969.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057969.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC
    AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG
    AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT
    GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC
    TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA
    GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG
    CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA
    GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG
    CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG
    AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG
    GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG
    GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG
    TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT
    CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC
    GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC
    CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA
    AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG
    CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG
    GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG
    GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT
    CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT
    AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG
    AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG
    CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA
    GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA
    AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT
    AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG
    AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC
    GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA
    TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG
    GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG
    CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA
    AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA
    CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT
    CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT
    GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG
    AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC
    AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC
    TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC
    CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG
    AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG
    CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT
    TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT
    TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG
    ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA
    CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC
    AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA
    CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG
    GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA
    ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG
    GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG
    GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA
    GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT
    AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA
    TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG
    CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG
    CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT
    CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG
    TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC
    ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC
    AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa10305
    Clone ID Related Accession (Same CDS sequence) NM_206147.4
    Accession Version NM_206147.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Rho guanine nucleotide exchange factor 2, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206147.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGCTCTG GGACAGACTC CGAATTTGGG AAAAAACAAG
    CATCGACGCG TTGGTTCTTC ACCGGACAAT ATGCATCCAC GTCATCCAGA TCGGATAACG
    AAAACGACTT CGGGCTCGTG GGAGATTGTT GAAAAGGATG GCGAATCCTC CCCGCCCGGA
    ACACCGCCGC CTCCATATCT ATCCAGCTCC CACATGACCG TGCTGGAAGA TCCGAATGAG
    AACAATCGTG GAGCAGCAGC AGCCGGACCT GGAGTCTTTA TCGAGTCGCA TCAGTTTACG
    CCGATGGCGG GAGCCTCTTC TCCGATCCCG ATATCCTTAC ATTCCAGCCA CATGCATGCG
    GCCCAGTCGA ACGATACGCA GAAGGAGATC ATCTCGATGG AGGACGAAAA CTCGGACTTG
    GATGAGCCCT TCATTGACGA GAACGGACCC TTTAACAATC TAACTCGTTT GTTAGAGGCC
    GAGAACGTCA CTTTCCTAGC CATCTTCCTA AACTACGTGA TCTCAAACTC GGATCCCGCG
    CCACTTCTGT TTTACCTGAT TACTGAGTTG TACAAGGAGG GCACCTCCAA GGACATGCGG
    AAATGGGCCT ACGAAATCCA CTCCACATTC CTCGTGCCGC GGGCTCCATT GTCATGGTAT
    CGCCAAGATG AATCGCTGGC CCGCGAGGTG GATAATGTCC TGCAGTTGGA GTATGACAAA
    GTGGAGATCC TAAGGACAGT TTTTCTGCGT AGCCGAAAGC GGGCCAAGGA CCTAATCAGT
    GAGCAGCTGC GTGAGTTTCA GCAGAAGCGC ACCGCCGGCC TGGGAACCAT TTACGGACCC
    ACGGACGACA AGCTGGCCGA GGCGAAGACG GATAAGCTAA GGGAGCAAAT CATCGACAAA
    TATTTAATGC CCAATCTTCA CGCGCTTATT GAGGATGAGA ACGGTTCACC GCCGGAGGAT
    GTACGCAAGG TGGCGTTGTG TTCTGCTCTT TCCACCGTCA TCTACCGTAT TTTCAACACT
    CGTCCGCCTC CAAGCAGCAT CGTTGAGCGG GTCCATCACT TTGTGAGCAG GGACAAGAGT
    TTCAAGTCGC GTATTATGGG CAAAAATCGC AAGATGAATG TTCGTGGTCA TCCATTGGTA
    TTGCGTCAAT ACTATGAAGT GACGCACTGC AATCATTGTC AGACGATTAT CTGGGGCGTG
    AGCCCGCAAG GTTATCATTG TACAGACTGT AAATTGAACA TACACCGTCA GTGCTCGAAA
    GTAGTGGACG AGAGTTGTCC CGGTCCCTTG CCCCAGGCAA AACGTCTCGC CCACAACGAC
    AAGATCAGTA AATTCATGGG CAAAATTCGA CCGCGTACCA GCGACGTCAT TGGAAATGAA
    AAGCGAAGTC GTCAAGATGA GGAATTGGAT GTTGAGTTGA CTCCAGACCG TGGTCAGGCG
    TCGATTGTGC GTCAACCCTC TGATCGGCGA CCGGATGCGA ACATATCGAT AAGGTCGAAT
    GGAAATACCT CCTGCAACAC TTCGGGGCTG AACACCACCG ACCTGCAAAG TTCTTTTCAC
    GGCAGCTGTG CCAACGACAG TATTAACCCC GGCGGTGGAG CCGGATGCAA CATGGATTTA
    TCCACGAGCG TGGCGTCAAC GACTCCGTCG ACCAGTGGAT CCGTGGCAGC CGGTCTGAGT
    GCTTTTGCCG AACTGAACGC CCTGGATACA GTGGATAAAG AAGCGCGTAG GGAGCGTTAC
    AGTCAGCATC CGAAGCACAA AAGTGCACCA GTCTCTGTGA ATCGGTCGGA ATCCTACAAG
    GAGCGCTTGT CCAACAAGAG GAACCGCAAC AGTCGCCGCA AGACCTCTGA TCCAAGCTTG
    TCGTCGCGTC CCAATGATGA ACAACTGGAC TTGGGTCTAT CGAATGCCAC TTATGTGGGC
    AGTTCGAATT CTAGTCTCTC TTCAGCTGGC GGCAGCGAGA GTCCCAGCAC GTCAATGGAG
    CACTTTGCTG CACCCGGAGC AGCGGGTGGC GTCCAGGTGC CGCCAATGGG ATTGAACCAG
    AACCAGCACC CCCATCTGCT CATCCAGCAG CACGCCCAGC AGTACTGCCA GCAGGATTCC
    TTTCAGGCGG GTTTGGCAGG GGCCGCTGGG AGCAGTGCAG CTAGCAACTC TAGTTTCTGG
    AATGCTGGCC ACCCATTGCC TGTAGCTCGT TGGACGCTGG AGAGCGAGGA TGAAGACGAC
    GTGAACGAGG CGGACTGGAG TTCCATGGTG GCCGCAGAGG TGTTGGCAGC CTTAACGGAC
    GCTGAGAAGA AGCGTCAGGA GATTATAAAT GAAATCTATC AAACTGAACG CAACCATGTG
    CGCACCCTAA AGCTGCTGGA TCGATTATTC TTCCTGCCAC TCTACGAGAG TGGATTGCTG
    TCCCAGGATC ATTTGCTGTT GTTGTTCCCG CCCGCCTTGC TGTCGCTCCG TGAGATTCAT
    GGCGCCTTCG AGCAGAGTCT TAAGCAACGA CGCATCGAGC ATAACCACGT GGTGAACACC
    ATAGGGGATC TGCTCGCCGA CATGTTCGAT GGTCAGTCTG GAGTTGTTCT TTGCGAGTTT
    GCGGCTCAGT TCTGTGCCCG CCAGCAAATC GCCTTGGAGG CACTCAAGGA GAAGCGCAAC
    AAGGACGAGA TGCTGCAAAA GCTATTGAAA AAGTCGGAGT CACACAAGGC ATGTCGCCGC
    CTCGAGCTAA AGGACTTGCT GCCCACCGTG CTGCAGCGCC TCACCAAGTA TCCGCTTTTG
    TTTGAGAACC TTTACAAGGT GACCGTGCGC TTGCTGCCAG AAAACACCAC CGAGGCGGAA
    GCCATTCAAC GGGCAGTGGA ATCCTCTAAA AGGATTCTTG TCGAGGTCAA CCAGGCAGTA
    AAGACAGCAG AGGATGCTCA CAAGCTGCAA AACATTCAGC GTAAGTTAGA CAGATCCTCC
    TATGACAAGG AAGAGTTTAA GAAATTGGAC CTGACCCAAC ATCACCTCAT CCATGACGGC
    AATCTGACGA TCAAGAAGAA TCCTAGCGTG CAGCTACATG GACTTCTGTT TGAAAACATG
    ATTGTTTTGC TGACCAAGCA GGATGATAAA TATTATCTAA AGAACTTGCA CACCCCGCTA
    TCGATCACCA ATAAGCCAGT CAGTCCAATT ATGAGCATTG ATGCGGACAC TTTGATCCGG
    CAGGAGGCGG CTGATAAAAA TTCCTTTTTC CTCATCAAGA TGAAGACATC ACAAATGTTG
    GAGCTACGCG CGCCTAGTAG CTCGGAGTGC AAGACATGGT TTAAACACTT CTCGGATGTG
    GCTGCTCGTC AGTCTAAGAA TCGTTCAAAG AACGCATCAA GCAACCATGA CACGAGTATT
    AGTGATCCAG CTCTCGCCGC TATTCCGCAT TCCAACACCA AAGAGTCGTT GGAGTTGAGC
    ACTGATACAG TACAGCCATT GGCTGCGACA GCCACATTGA CTACCACACC ATTGGCCCCA
    ATGCTGCCTA TAGCCACGGT TACACCGGCT CCAGCGACCA ATAATAGTAA CGTTAGCTCT
    CTTACTGGAG TTCAGTTGCG AAACCCTCAA CGGGATGCGA CAGCAAGTGA ATCTGATGCG
    GATTATGTAA ACACACCAAA GCCGCGTTCG AGCCAAAATG AAGTTAATCG CACTATGTCC
    ATAAGAAGCA CTGGCGAACC CATTCAGAAG TATTCGGCGA ATGGGACGGA AGCAAACGAC
    GTTACTTTAC GACACTCTCA GTCGACTAGG GAATCGGTTA GACCAGGATC TACTGGGGAG
    GAGCGAAACT CCACGTATGG TATGGTTGGA GGTAACTCCA AACGCGACAG CGCCAGCATT
    GTCTGCTCGA ACAACTCGAA CAACACGCGC ACCCTTCTGA TGCAGAGTCC CTTGGTGGAC
    CCTACGGCCA TTCAGGTCAG CATTAGTCCC GCTCACACAG CGGAACCTGT GTTGACACCA
    GGAGAGAAGT TACGCCGCTT GGACGCCTCC ATTAGGAATG ATTTGCTGGA GAAGCAGAAA
    ATCATTTGTG ATATCTTCCG TTTGCCAGTG GAACACTACG ACCAGATTGT GGACATTGCC
    ATGATGCCAG AGGCGCCGAA AGACAGTGCA GATATTGCTT TAGCTGCTTA CGATCAGATT
    CAAACCCTGA CCAAGATGCT GAACGAGTAC ATGCACGTCA CGCCTGAGCA AGAGGTCTCA
    GCGGTGTCCA CGGCGGTTTG TGGCCACTGT CACGAGAAGG AAAAGCTTCG TAAGAAGGTG
    GCACCATCCT CATCGTTTTC TTCATCACCA CCACCGCTGC CGCCTCCCAA TAGGCAGCAT
    GCCCAGGCTC AGGCCCAGAT ACCGCCATCG CGGCTGATGC CCAAACTACA AACTCTTGAT
    CTTGACGAAG TTGCCATACA CGAAGACGAT GACGGATACT GTGAGATCGA CGAACTGCGC
    TTACCGGCTA TTCCGTCCAA ACCACATGAG CGGCCCACAA CGCCACTGGC TCCTTTCAAT
    ACTGAGCCGA AAACTTCACA ATCTGTTATA GATGCCTCGA AACGTCAATC CACTGATGCC
    GTTCCGGAGG GATTACTGGA ACAAGAACCA CTCGAAGGCG ATAAGACGGA GACCAAGGGT
    GAAGATAATG AAGTGAAAAC TGTGCCGTCA GATAAGCTAA GTGAATCATG CAATGAAGAG
    AGGCAATGTG TGGAGGCGGA TATCACAAAG GAAGTGGCAG ATCCAACGAC CTCTAAGAAT
    GAAGCTGCAG CATCGGTGGA TGAATTACCA AGCCAGAGCC GGGAGATAAA AACGGCTGAA
    AACGCAAGCA AATCTGTAGC TGACAAAAAG GAAGACAACG AGGAAACCAT CGAAGAAGGT
    GTGGCATCCA CGGTCGATAG CTCCACTCAA ACATCACCAA CTGAATCGCC GAAAGAGACG
    GATAAGTTAA CTGGAGGATC GAGCAGCACC TGTGGGCCGA ATCGCATTCA GCACGCTAGT
    GTGCTGGAGC CGAGTGTGCC CTGCCATGCA CTCAGCAGCA TTGTAACAAT ACTGAATGAG
    CAGATTTCCA TGCTTTTGCC AAAAATTAAC GAACGCGATA TGGAAAGGGA GCGATTGCGT
    AAAGAGAATC AACACCTTCG CGAGCTCTTG AGTGCGCTGC ATGATCGACA GCGAGTTGAT
    GAAGTAAAGG AAACTCCGTT TGATCTAAAG AAGCTGATGC ATGCTGAGGA TGTAGAGTTT
    GACGATGATA TTGACGCCAT TTCCAACAGT TCGCTGACGC CAACGCCTAC GCCGATTCCC
    ACAGCATCAC CAAGCGCCAG CGGCCAGGTA GAGACAGCGG AAGCCATGAG GATTACTAGC
    ACTGAGGATG AGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995869.1
    CDS474..7091
    Translation

    Target ORF information:

    RefSeq Version NM_206147.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206147.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGCTCTG GGACAGACTC CGAATTTGGG AAAAAACAAG
    CATCGACGCG TTGGTTCTTC ACCGGACAAT ATGCATCCAC GTCATCCAGA TCGGATAACG
    AAAACGACTT CGGGCTCGTG GGAGATTGTT GAAAAGGATG GCGAATCCTC CCCGCCCGGA
    ACACCGCCGC CTCCATATCT ATCCAGCTCC CACATGACCG TGCTGGAAGA TCCGAATGAG
    AACAATCGTG GAGCAGCAGC AGCCGGACCT GGAGTCTTTA TCGAGTCGCA TCAGTTTACG
    CCGATGGCGG GAGCCTCTTC TCCGATCCCG ATATCCTTAC ATTCCAGCCA CATGCATGCG
    GCCCAGTCGA ACGATACGCA GAAGGAGATC ATCTCGATGG AGGACGAAAA CTCGGACTTG
    GATGAGCCCT TCATTGACGA GAACGGACCC TTTAACAATC TAACTCGTTT GTTAGAGGCC
    GAGAACGTCA CTTTCCTAGC CATCTTCCTA AACTACGTGA TCTCAAACTC GGATCCCGCG
    CCACTTCTGT TTTACCTGAT TACTGAGTTG TACAAGGAGG GCACCTCCAA GGACATGCGG
    AAATGGGCCT ACGAAATCCA CTCCACATTC CTCGTGCCGC GGGCTCCATT GTCATGGTAT
    CGCCAAGATG AATCGCTGGC CCGCGAGGTG GATAATGTCC TGCAGTTGGA GTATGACAAA
    GTGGAGATCC TAAGGACAGT TTTTCTGCGT AGCCGAAAGC GGGCCAAGGA CCTAATCAGT
    GAGCAGCTGC GTGAGTTTCA GCAGAAGCGC ACCGCCGGCC TGGGAACCAT TTACGGACCC
    ACGGACGACA AGCTGGCCGA GGCGAAGACG GATAAGCTAA GGGAGCAAAT CATCGACAAA
    TATTTAATGC CCAATCTTCA CGCGCTTATT GAGGATGAGA ACGGTTCACC GCCGGAGGAT
    GTACGCAAGG TGGCGTTGTG TTCTGCTCTT TCCACCGTCA TCTACCGTAT TTTCAACACT
    CGTCCGCCTC CAAGCAGCAT CGTTGAGCGG GTCCATCACT TTGTGAGCAG GGACAAGAGT
    TTCAAGTCGC GTATTATGGG CAAAAATCGC AAGATGAATG TTCGTGGTCA TCCATTGGTA
    TTGCGTCAAT ACTATGAAGT GACGCACTGC AATCATTGTC AGACGATTAT CTGGGGCGTG
    AGCCCGCAAG GTTATCATTG TACAGACTGT AAATTGAACA TACACCGTCA GTGCTCGAAA
    GTAGTGGACG AGAGTTGTCC CGGTCCCTTG CCCCAGGCAA AACGTCTCGC CCACAACGAC
    AAGATCAGTA AATTCATGGG CAAAATTCGA CCGCGTACCA GCGACGTCAT TGGAAATGAA
    AAGCGAAGTC GTCAAGATGA GGAATTGGAT GTTGAGTTGA CTCCAGACCG TGGTCAGGCG
    TCGATTGTGC GTCAACCCTC TGATCGGCGA CCGGATGCGA ACATATCGAT AAGGTCGAAT
    GGAAATACCT CCTGCAACAC TTCGGGGCTG AACACCACCG ACCTGCAAAG TTCTTTTCAC
    GGCAGCTGTG CCAACGACAG TATTAACCCC GGCGGTGGAG CCGGATGCAA CATGGATTTA
    TCCACGAGCG TGGCGTCAAC GACTCCGTCG ACCAGTGGAT CCGTGGCAGC CGGTCTGAGT
    GCTTTTGCCG AACTGAACGC CCTGGATACA GTGGATAAAG AAGCGCGTAG GGAGCGTTAC
    AGTCAGCATC CGAAGCACAA AAGTGCACCA GTCTCTGTGA ATCGGTCGGA ATCCTACAAG
    GAGCGCTTGT CCAACAAGAG GAACCGCAAC AGTCGCCGCA AGACCTCTGA TCCAAGCTTG
    TCGTCGCGTC CCAATGATGA ACAACTGGAC TTGGGTCTAT CGAATGCCAC TTATGTGGGC
    AGTTCGAATT CTAGTCTCTC TTCAGCTGGC GGCAGCGAGA GTCCCAGCAC GTCAATGGAG
    CACTTTGCTG CACCCGGAGC AGCGGGTGGC GTCCAGGTGC CGCCAATGGG ATTGAACCAG
    AACCAGCACC CCCATCTGCT CATCCAGCAG CACGCCCAGC AGTACTGCCA GCAGGATTCC
    TTTCAGGCGG GTTTGGCAGG GGCCGCTGGG AGCAGTGCAG CTAGCAACTC TAGTTTCTGG
    AATGCTGGCC ACCCATTGCC TGTAGCTCGT TGGACGCTGG AGAGCGAGGA TGAAGACGAC
    GTGAACGAGG CGGACTGGAG TTCCATGGTG GCCGCAGAGG TGTTGGCAGC CTTAACGGAC
    GCTGAGAAGA AGCGTCAGGA GATTATAAAT GAAATCTATC AAACTGAACG CAACCATGTG
    CGCACCCTAA AGCTGCTGGA TCGATTATTC TTCCTGCCAC TCTACGAGAG TGGATTGCTG
    TCCCAGGATC ATTTGCTGTT GTTGTTCCCG CCCGCCTTGC TGTCGCTCCG TGAGATTCAT
    GGCGCCTTCG AGCAGAGTCT TAAGCAACGA CGCATCGAGC ATAACCACGT GGTGAACACC
    ATAGGGGATC TGCTCGCCGA CATGTTCGAT GGTCAGTCTG GAGTTGTTCT TTGCGAGTTT
    GCGGCTCAGT TCTGTGCCCG CCAGCAAATC GCCTTGGAGG CACTCAAGGA GAAGCGCAAC
    AAGGACGAGA TGCTGCAAAA GCTATTGAAA AAGTCGGAGT CACACAAGGC ATGTCGCCGC
    CTCGAGCTAA AGGACTTGCT GCCCACCGTG CTGCAGCGCC TCACCAAGTA TCCGCTTTTG
    TTTGAGAACC TTTACAAGGT GACCGTGCGC TTGCTGCCAG AAAACACCAC CGAGGCGGAA
    GCCATTCAAC GGGCAGTGGA ATCCTCTAAA AGGATTCTTG TCGAGGTCAA CCAGGCAGTA
    AAGACAGCAG AGGATGCTCA CAAGCTGCAA AACATTCAGC GTAAGTTAGA CAGATCCTCC
    TATGACAAGG AAGAGTTTAA GAAATTGGAC CTGACCCAAC ATCACCTCAT CCATGACGGC
    AATCTGACGA TCAAGAAGAA TCCTAGCGTG CAGCTACATG GACTTCTGTT TGAAAACATG
    ATTGTTTTGC TGACCAAGCA GGATGATAAA TATTATCTAA AGAACTTGCA CACCCCGCTA
    TCGATCACCA ATAAGCCAGT CAGTCCAATT ATGAGCATTG ATGCGGACAC TTTGATCCGG
    CAGGAGGCGG CTGATAAAAA TTCCTTTTTC CTCATCAAGA TGAAGACATC ACAAATGTTG
    GAGCTACGCG CGCCTAGTAG CTCGGAGTGC AAGACATGGT TTAAACACTT CTCGGATGTG
    GCTGCTCGTC AGTCTAAGAA TCGTTCAAAG AACGCATCAA GCAACCATGA CACGAGTATT
    AGTGATCCAG CTCTCGCCGC TATTCCGCAT TCCAACACCA AAGAGTCGTT GGAGTTGAGC
    ACTGATACAG TACAGCCATT GGCTGCGACA GCCACATTGA CTACCACACC ATTGGCCCCA
    ATGCTGCCTA TAGCCACGGT TACACCGGCT CCAGCGACCA ATAATAGTAA CGTTAGCTCT
    CTTACTGGAG TTCAGTTGCG AAACCCTCAA CGGGATGCGA CAGCAAGTGA ATCTGATGCG
    GATTATGTAA ACACACCAAA GCCGCGTTCG AGCCAAAATG AAGTTAATCG CACTATGTCC
    ATAAGAAGCA CTGGCGAACC CATTCAGAAG TATTCGGCGA ATGGGACGGA AGCAAACGAC
    GTTACTTTAC GACACTCTCA GTCGACTAGG GAATCGGTTA GACCAGGATC TACTGGGGAG
    GAGCGAAACT CCACGTATGG TATGGTTGGA GGTAACTCCA AACGCGACAG CGCCAGCATT
    GTCTGCTCGA ACAACTCGAA CAACACGCGC ACCCTTCTGA TGCAGAGTCC CTTGGTGGAC
    CCTACGGCCA TTCAGGTCAG CATTAGTCCC GCTCACACAG CGGAACCTGT GTTGACACCA
    GGAGAGAAGT TACGCCGCTT GGACGCCTCC ATTAGGAATG ATTTGCTGGA GAAGCAGAAA
    ATCATTTGTG ATATCTTCCG TTTGCCAGTG GAACACTACG ACCAGATTGT GGACATTGCC
    ATGATGCCAG AGGCGCCGAA AGACAGTGCA GATATTGCTT TAGCTGCTTA CGATCAGATT
    CAAACCCTGA CCAAGATGCT GAACGAGTAC ATGCACGTCA CGCCTGAGCA AGAGGTCTCA
    GCGGTGTCCA CGGCGGTTTG TGGCCACTGT CACGAGAAGG AAAAGCTTCG TAAGAAGGTG
    GCACCATCCT CATCGTTTTC TTCATCACCA CCACCGCTGC CGCCTCCCAA TAGGCAGCAT
    GCCCAGGCTC AGGCCCAGAT ACCGCCATCG CGGCTGATGC CCAAACTACA AACTCTTGAT
    CTTGACGAAG TTGCCATACA CGAAGACGAT GACGGATACT GTGAGATCGA CGAACTGCGC
    TTACCGGCTA TTCCGTCCAA ACCACATGAG CGGCCCACAA CGCCACTGGC TCCTTTCAAT
    ACTGAGCCGA AAACTTCACA ATCTGTTATA GATGCCTCGA AACGTCAATC CACTGATGCC
    GTTCCGGAGG GATTACTGGA ACAAGAACCA CTCGAAGGCG ATAAGACGGA GACCAAGGGT
    GAAGATAATG AAGTGAAAAC TGTGCCGTCA GATAAGCTAA GTGAATCATG CAATGAAGAG
    AGGCAATGTG TGGAGGCGGA TATCACAAAG GAAGTGGCAG ATCCAACGAC CTCTAAGAAT
    GAAGCTGCAG CATCGGTGGA TGAATTACCA AGCCAGAGCC GGGAGATAAA AACGGCTGAA
    AACGCAAGCA AATCTGTAGC TGACAAAAAG GAAGACAACG AGGAAACCAT CGAAGAAGGT
    GTGGCATCCA CGGTCGATAG CTCCACTCAA ACATCACCAA CTGAATCGCC GAAAGAGACG
    GATAAGTTAA CTGGAGGATC GAGCAGCACC TGTGGGCCGA ATCGCATTCA GCACGCTAGT
    GTGCTGGAGC CGAGTGTGCC CTGCCATGCA CTCAGCAGCA TTGTAACAAT ACTGAATGAG
    CAGATTTCCA TGCTTTTGCC AAAAATTAAC GAACGCGATA TGGAAAGGGA GCGATTGCGT
    AAAGAGAATC AACACCTTCG CGAGCTCTTG AGTGCGCTGC ATGATCGACA GCGAGTTGAT
    GAAGTAAAGG AAACTCCGTT TGATCTAAAG AAGCTGATGC ATGCTGAGGA TGTAGAGTTT
    GACGATGATA TTGACGCCAT TTCCAACAGT TCGCTGACGC CAACGCCTAC GCCGATTCCC
    ACAGCATCAC CAAGCGCCAG CGGCCAGGTA GAGACAGCGG AAGCCATGAG GATTACTAGC
    ACTGAGGATG AGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12368
    Clone ID Related Accession (Same CDS sequence) NM_001259452.2
    Accession Version NM_001259452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Rho guanine nucleotide exchange factor 2, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259452.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTC AGCATCCGAA GCACAAAAGT
    GCACCAGTCT CTGTGAATCG GTCGGAATCC TACAAGGAGC GCTTGTCCAA CAAGAGGAAC
    CGCAACAGTC GCCGCAAGAC CTCTGATCCA AGCTTGTCGT CGCGTCCCAA TGATGAACAA
    CTGGACTTGG GTCTATCGAA TGCCACTTAT GTGGGCAGTT CGAATTCTAG TCTCTCTTCA
    GCTGGCGGCA GCGAGAGTCC CAGCACGTCA ATGGAGCACT TTGCTGCACC CGGAGCAGCG
    GGTGGCGTCC AGGTGCCGCC AATGGGATTG AACCAGAACC AGCACCCCCA TCTGCTCATC
    CAGCAGCACG CCCAGCAGTA CTGCCAGCAG GATTCCTTTC AGGCGGGTTT GGCAGGGGCC
    GCTGGGAGCA GTGCAGCTAG CAACTCTAGT TTCTGGAATG CTGGCCACCC ATTGCCTGTA
    GCTCGTTGGA CGCTGGAGAG CGAGGATGAA GACGACGTGA ACGAGGCGGA CTGGAGTTCC
    ATGGTGGCCG CAGAGGTGTT GGCAGCCTTA ACGGACGCTG AGAAGAAGCG TCAGGAGATT
    ATAAATGAAA TCTATCAAAC TGAACGCAAC CATGTGCGCA CCCTAAAGCT GCTGGATCGA
    TTATTCTTCC TGCCACTCTA CGAGAGTGGA TTGCTGTCCC AGGATCATTT GCTGTTGTTG
    TTCCCGCCCG CCTTGCTGTC GCTCCGTGAG ATTCATGGCG CCTTCGAGCA GAGTCTTAAG
    CAACGACGCA TCGAGCATAA CCACGTGGTG AACACCATAG GGGATCTGCT CGCCGACATG
    TTCGATGGTC AGTCTGGAGT TGTTCTTTGC GAGTTTGCGG CTCAGTTCTG TGCCCGCCAG
    CAAATCGCCT TGGAGGCACT CAAGGAGAAG CGCAACAAGG ACGAGATGCT GCAAAAGCTA
    TTGAAAAAGT CGGAGTCACA CAAGGCATGT CGCCGCCTCG AGCTAAAGGA CTTGCTGCCC
    ACCGTGCTGC AGCGCCTCAC CAAGTATCCG CTTTTGTTTG AGAACCTTTA CAAGGTGACC
    GTGCGCTTGC TGCCAGAAAA CACCACCGAG GCGGAAGCCA TTCAACGGGC AGTGGAATCC
    TCTAAAAGGA TTCTTGTCGA GGTCAACCAG GCAGTAAAGA CAGCAGAGGA TGCTCACAAG
    CTGCAAAACA TTCAGCGTAA GTTAGACAGA TCCTCCTATG ACAAGGAAGA GTTTAAGAAA
    TTGGACCTGA CCCAACATCA CCTCATCCAT GACGGCAATC TGACGATCAA GAAGAATCCT
    AGCGTGCAGC TACATGGACT TCTGTTTGAA AACATGATTG TTTTGCTGAC CAAGCAGGAT
    GATAAATATT ATCTAAAGAA CTTGCACACC CCGCTATCGA TCACCAATAA GCCAGTCAGT
    CCAATTATGA GCATTGATGC GGACACTTTG ATCCGGCAGG AGGCGGCTGA TAAAAATTCC
    TTTTTCCTCA TCAAGATGAA GACATCACAA ATGTTGGAGC TACGCGCGCC TAGTAGCTCG
    GAGTGCAAGA CATGGTTTAA ACACTTCTCG GATGTGGCTG CTCGTCAGTC TAAGAATCGT
    TCAAAGAACG CATCAAGCAA CCATGACACG AGTATTAGTG ATCCAGCTCT CGCCGCTATT
    CCGCATTCCA ACACCAAAGA GTCGTTGGAG TTGAGCACTG ATACAGTACA GCCATTGGCT
    GCGACAGCCA CATTGACTAC CACACCATTG GCCCCAATGC TGCCTATAGC CACGGTTACA
    CCGGCTCCAG CGACCAATAA TAGTAACGTT AGCTCTCTTA CTGGAGTTCA GTTGCGAAAC
    CCTCAACGGG ATGCGACAGC AAGTGAATCT GATGCGGATT ATGTAAACAC ACCAAAGCCG
    CGTTCGAGCC AAAATGAAGT TAATCGCACT ATGTCCATAA GAAGCACTGG CGAACCCATT
    CAGAAGTATT CGGCGAATGG GACGGAAGCA AACGACGTTA CTTTACGACA CTCTCAGTCG
    ACTAGGGAAT CGGTTAGACC AGGATCTACT GGGGAGGAGC GAAACTCCAC GTATGGTATG
    GTTGGAGGTA ACTCCAAACG CGACAGCGCC AGCATTGTCT GCTCGAACAA CTCGAACAAC
    ACGCGCACCC TTCTGATGCA GAGTCCCTTG GTGGACCCTA CGGCCATTCA GGTCAGCATT
    AGTCCCGCTC ACACAGCGGA ACCTGTGTTG ACACCAGGAG AGAAGTTACG CCGCTTGGAC
    GCCTCCATTA GGAATGATTT GCTGGAGAAG CAGAAAATCA TTTGTGATAT CTTCCGTTTG
    CCAGTGGAAC ACTACGACCA GATTGTGGAC ATTGCCATGA TGCCAGAGGC GCCGAAAGAC
    AGTGCAGATA TTGCTTTAGC TGCTTACGAT CAGATTCAAA CCCTGACCAA GATGCTGAAC
    GAGTACATGC ACGTCACGCC TGAGCAAGAG GTCTCAGCGG TGTCCACGGC GGTTTGTGGC
    CACTGTCACG AGAAGGAAAA GCTTCGTAAG AAGGTGGCAC CATCCTCATC GTTTTCTTCA
    TCACCACCAC CGCTGCCGCC TCCCAATAGG CAGCATGCCC AGGCTCAGGC CCAGATACCG
    CCATCGCGGC TGATGCCCAA ACTACAAACT CTTGATCTTG ACGAAGTTGC CATACACGAA
    GACGATGACG GATACTGTGA GATCGACGAA CTGCGCTTAC CGGCTATTCC GTCCAAACCA
    CATGAGCGGC CCACAACGCC ACTGGCTCCT TTCAATACTG AGCCGAAAAC TTCACAATCT
    GTTATAGATG CCTCGAAACG TCAATCCACT GATGCCGTTC CGGAGGGATT ACTGGAACAA
    GAACCACTCG AAGGCGATAA GACGGAGACC AAGGGTGAAG ATAATGAAGT GAAAACTGTG
    CCGTCAGATA AGCTAAGTGA ATCATGCAAT GAAGAGAGGC AATGTGTGGA GGCGGATATC
    ACAAAGGAAG TGGCAGATCC AACGACCTCT AAGAATGAAG CTGCAGCATC GGTGGATGAA
    TTACCAAGCC AGAGCCGGGA GATAAAAACG GCTGAAAACG CAAGCAAATC TGTAGCTGAC
    AAAAAGGAAG ACAACGAGGA AACCATCGAA GAAGGTGTGG CATCCACGGT CGATAGCTCC
    ACTCAAACAT CACCAACTGA ATCGCCGAAA GAGACGGATA AGTTAACTGG AGGATCGAGC
    AGCACCTGTG GGCCGAATCG CATTCAGCAC GCTAGTGTGC TGGAGCCGAG TGTGCCCTGC
    CATGCACTCA GCAGCATTGT AACAATACTG AATGAGCAGA TTTCCATGCT TTTGCCAAAA
    ATTAACGAAC GCGATATGGA AAGGGAGCGA TTGCGTAAAG AGAATCAACA CCTTCGCGAG
    CTCTTGAGTG CGCTGCATGA TCGACAGCGA GTTGATGAAG TAAAGGAAAC TCCGTTTGAT
    CTAAAGAAGC TGATGCATGC TGAGGATGTA GAGTTTGACG ATGATATTGA CGCCATTTCC
    AACAGTTCGC TGACGCCAAC GCCTACGCCG ATTCCCACAG CATCACCAAG CGCCAGCGGC
    CAGGTAGAGA CAGCGGAAGC CATGAGGATT ACTAGCACTG AGGATGAGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246381.1
    CDS474..8147
    Translation

    Target ORF information:

    RefSeq Version NM_001259452.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC
    AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT
    CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT
    CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC
    CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA
    TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG
    TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT
    CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG
    TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT
    CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT
    CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA
    CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC
    AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG
    GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA
    CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG
    AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA
    ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT
    GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC
    ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT
    TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG
    TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT
    CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA
    GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT
    TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG
    CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC
    GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA
    GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG
    ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC
    CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG
    GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA
    GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT
    CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC
    GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT
    CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG
    TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC
    ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG
    GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA
    GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT
    TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT
    CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG
    GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT
    GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC
    TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC
    ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC
    AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA
    ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC
    GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTC AGCATCCGAA GCACAAAAGT
    GCACCAGTCT CTGTGAATCG GTCGGAATCC TACAAGGAGC GCTTGTCCAA CAAGAGGAAC
    CGCAACAGTC GCCGCAAGAC CTCTGATCCA AGCTTGTCGT CGCGTCCCAA TGATGAACAA
    CTGGACTTGG GTCTATCGAA TGCCACTTAT GTGGGCAGTT CGAATTCTAG TCTCTCTTCA
    GCTGGCGGCA GCGAGAGTCC CAGCACGTCA ATGGAGCACT TTGCTGCACC CGGAGCAGCG
    GGTGGCGTCC AGGTGCCGCC AATGGGATTG AACCAGAACC AGCACCCCCA TCTGCTCATC
    CAGCAGCACG CCCAGCAGTA CTGCCAGCAG GATTCCTTTC AGGCGGGTTT GGCAGGGGCC
    GCTGGGAGCA GTGCAGCTAG CAACTCTAGT TTCTGGAATG CTGGCCACCC ATTGCCTGTA
    GCTCGTTGGA CGCTGGAGAG CGAGGATGAA GACGACGTGA ACGAGGCGGA CTGGAGTTCC
    ATGGTGGCCG CAGAGGTGTT GGCAGCCTTA ACGGACGCTG AGAAGAAGCG TCAGGAGATT
    ATAAATGAAA TCTATCAAAC TGAACGCAAC CATGTGCGCA CCCTAAAGCT GCTGGATCGA
    TTATTCTTCC TGCCACTCTA CGAGAGTGGA TTGCTGTCCC AGGATCATTT GCTGTTGTTG
    TTCCCGCCCG CCTTGCTGTC GCTCCGTGAG ATTCATGGCG CCTTCGAGCA GAGTCTTAAG
    CAACGACGCA TCGAGCATAA CCACGTGGTG AACACCATAG GGGATCTGCT CGCCGACATG
    TTCGATGGTC AGTCTGGAGT TGTTCTTTGC GAGTTTGCGG CTCAGTTCTG TGCCCGCCAG
    CAAATCGCCT TGGAGGCACT CAAGGAGAAG CGCAACAAGG ACGAGATGCT GCAAAAGCTA
    TTGAAAAAGT CGGAGTCACA CAAGGCATGT CGCCGCCTCG AGCTAAAGGA CTTGCTGCCC
    ACCGTGCTGC AGCGCCTCAC CAAGTATCCG CTTTTGTTTG AGAACCTTTA CAAGGTGACC
    GTGCGCTTGC TGCCAGAAAA CACCACCGAG GCGGAAGCCA TTCAACGGGC AGTGGAATCC
    TCTAAAAGGA TTCTTGTCGA GGTCAACCAG GCAGTAAAGA CAGCAGAGGA TGCTCACAAG
    CTGCAAAACA TTCAGCGTAA GTTAGACAGA TCCTCCTATG ACAAGGAAGA GTTTAAGAAA
    TTGGACCTGA CCCAACATCA CCTCATCCAT GACGGCAATC TGACGATCAA GAAGAATCCT
    AGCGTGCAGC TACATGGACT TCTGTTTGAA AACATGATTG TTTTGCTGAC CAAGCAGGAT
    GATAAATATT ATCTAAAGAA CTTGCACACC CCGCTATCGA TCACCAATAA GCCAGTCAGT
    CCAATTATGA GCATTGATGC GGACACTTTG ATCCGGCAGG AGGCGGCTGA TAAAAATTCC
    TTTTTCCTCA TCAAGATGAA GACATCACAA ATGTTGGAGC TACGCGCGCC TAGTAGCTCG
    GAGTGCAAGA CATGGTTTAA ACACTTCTCG GATGTGGCTG CTCGTCAGTC TAAGAATCGT
    TCAAAGAACG CATCAAGCAA CCATGACACG AGTATTAGTG ATCCAGCTCT CGCCGCTATT
    CCGCATTCCA ACACCAAAGA GTCGTTGGAG TTGAGCACTG ATACAGTACA GCCATTGGCT
    GCGACAGCCA CATTGACTAC CACACCATTG GCCCCAATGC TGCCTATAGC CACGGTTACA
    CCGGCTCCAG CGACCAATAA TAGTAACGTT AGCTCTCTTA CTGGAGTTCA GTTGCGAAAC
    CCTCAACGGG ATGCGACAGC AAGTGAATCT GATGCGGATT ATGTAAACAC ACCAAAGCCG
    CGTTCGAGCC AAAATGAAGT TAATCGCACT ATGTCCATAA GAAGCACTGG CGAACCCATT
    CAGAAGTATT CGGCGAATGG GACGGAAGCA AACGACGTTA CTTTACGACA CTCTCAGTCG
    ACTAGGGAAT CGGTTAGACC AGGATCTACT GGGGAGGAGC GAAACTCCAC GTATGGTATG
    GTTGGAGGTA ACTCCAAACG CGACAGCGCC AGCATTGTCT GCTCGAACAA CTCGAACAAC
    ACGCGCACCC TTCTGATGCA GAGTCCCTTG GTGGACCCTA CGGCCATTCA GGTCAGCATT
    AGTCCCGCTC ACACAGCGGA ACCTGTGTTG ACACCAGGAG AGAAGTTACG CCGCTTGGAC
    GCCTCCATTA GGAATGATTT GCTGGAGAAG CAGAAAATCA TTTGTGATAT CTTCCGTTTG
    CCAGTGGAAC ACTACGACCA GATTGTGGAC ATTGCCATGA TGCCAGAGGC GCCGAAAGAC
    AGTGCAGATA TTGCTTTAGC TGCTTACGAT CAGATTCAAA CCCTGACCAA GATGCTGAAC
    GAGTACATGC ACGTCACGCC TGAGCAAGAG GTCTCAGCGG TGTCCACGGC GGTTTGTGGC
    CACTGTCACG AGAAGGAAAA GCTTCGTAAG AAGGTGGCAC CATCCTCATC GTTTTCTTCA
    TCACCACCAC CGCTGCCGCC TCCCAATAGG CAGCATGCCC AGGCTCAGGC CCAGATACCG
    CCATCGCGGC TGATGCCCAA ACTACAAACT CTTGATCTTG ACGAAGTTGC CATACACGAA
    GACGATGACG GATACTGTGA GATCGACGAA CTGCGCTTAC CGGCTATTCC GTCCAAACCA
    CATGAGCGGC CCACAACGCC ACTGGCTCCT TTCAATACTG AGCCGAAAAC TTCACAATCT
    GTTATAGATG CCTCGAAACG TCAATCCACT GATGCCGTTC CGGAGGGATT ACTGGAACAA
    GAACCACTCG AAGGCGATAA GACGGAGACC AAGGGTGAAG ATAATGAAGT GAAAACTGTG
    CCGTCAGATA AGCTAAGTGA ATCATGCAAT GAAGAGAGGC AATGTGTGGA GGCGGATATC
    ACAAAGGAAG TGGCAGATCC AACGACCTCT AAGAATGAAG CTGCAGCATC GGTGGATGAA
    TTACCAAGCC AGAGCCGGGA GATAAAAACG GCTGAAAACG CAAGCAAATC TGTAGCTGAC
    AAAAAGGAAG ACAACGAGGA AACCATCGAA GAAGGTGTGG CATCCACGGT CGATAGCTCC
    ACTCAAACAT CACCAACTGA ATCGCCGAAA GAGACGGATA AGTTAACTGG AGGATCGAGC
    AGCACCTGTG GGCCGAATCG CATTCAGCAC GCTAGTGTGC TGGAGCCGAG TGTGCCCTGC
    CATGCACTCA GCAGCATTGT AACAATACTG AATGAGCAGA TTTCCATGCT TTTGCCAAAA
    ATTAACGAAC GCGATATGGA AAGGGAGCGA TTGCGTAAAG AGAATCAACA CCTTCGCGAG
    CTCTTGAGTG CGCTGCATGA TCGACAGCGA GTTGATGAAG TAAAGGAAAC TCCGTTTGAT
    CTAAAGAAGC TGATGCATGC TGAGGATGTA GAGTTTGACG ATGATATTGA CGCCATTTCC
    AACAGTTCGC TGACGCCAAC GCCTACGCCG ATTCCCACAG CATCACCAAG CGCCAGCGGC
    CAGGTAGAGA CAGCGGAAGC CATGAGGATT ACTAGCACTG AGGATGAGGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21838
    Clone ID Related Accession (Same CDS sequence) NM_001299586.1
    Accession Version NM_001299586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Rho guanine nucleotide exchange factor 2, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGCAGCAA CGGATGAGCC ACCAAGCGGA GTCGATGTCT
    CAATCGATGC ATCAGCACAC CAGCACTCCG ACCTCGCAGC AGTTCTTCCA TCCGCATCAG
    CAACAACATC GCTTTAAGGA AACTGGACCG ACGTCGAAAG GCAAGAACAA GTTCCTAATT
    TCGAGGAGTT TGATCGAGGA GGATGTGCCG CCACCACTGC CGCAGAGGAA TCCGCCCAGG
    CAGTTAAATT TGGACCTGAA GAACGGAAAC GCGTCGCCGG GTGGGTCACA TCTAGTGGCT
    CCAGTTTCCG ATCTGGATCG CGCCACAAGT CCCCAATTAA ATAGATCCCA ACAACAGCAA
    CTGCCTAGCA GCACTGACAA CAGCCCGAGC AATGCCAAGT CCAAGCGTTC CAAGATCAAA
    ACGAAGGCCC TATCGGATCC TAAGATGTCC ACTCAGATGT TCCTGCAAAT GGAATCGGCG
    AGTGCAGCTG GAGCAGCCGG AGGTTCCATC GAGGTAGATG GGGGTCCACC ACCGCTACCG
    CCTCGCTTGC CTGGCATGAT GACGGAGGAT ATGAGCCGCG GCAGCTGTCA GAATCTCGCT
    CAACCCAATT CCGTGGGCAC TGCCTTCAAC TATCCTCTGG TGTCCACAAC AACAGCGGTG
    CAGAACGATA ACCTGAACAT TGCCTTTCCT TTGTCCCAAC GACCCAACAT TGTCCAGCAG
    CTGCAGCAAT ATCAACAGCA GCAGCAGCAT CAAATGAGCG GTGGCCAGGC CACAGGCGCT
    CTGGGACAGA CTCCGAATTT GGGAAAAAAC AAGCATCGAC GCGTTGGTTC TTCACCGGAC
    AATATGCATC CACGTCATCC AGATCGGATA ACGAAAACGA CTTCGGGCTC GTGGGAGATT
    GTTGAAAAGG ATGGCGAATC CTCCCCGCCC GGAACACCGC CGCCTCCATA TCTATCCAGC
    TCCCACATGA CCGTGCTGGA AGATCCGAAT GAGAACAATC GTGGAGCAGC AGCAGCCGGA
    CCTGGAGTCT TTATCGAGTC GCATCAGTTT ACGCCGATGG CGGGAGCCTC TTCTCCGATC
    CCGATATCCT TACATTCCAG CCACATGCAT GCGGCCCAGT CGAACGATAC GCAGAAGGAG
    ATCATCTCGA TGGAGGACGA AAACTCGGAC TTGGATGAGC CCTTCATTGA CGAGAACGGA
    CCCTTTAACA ATCTAACTCG TTTGTTAGAG GCCGAGAACG TCACTTTCCT AGCCATCTTC
    CTAAACTACG TGATCTCAAA CTCGGATCCC GCGCCACTTC TGTTTTACCT GATTACTGAG
    TTGTACAAGG AGGGCACCTC CAAGGACATG CGGAAATGGG CCTACGAAAT CCACTCCACA
    TTCCTCGTGC CGCGGGCTCC ATTGTCATGG TATCGCCAAG ATGAATCGCT GGCCCGCGAG
    GTGGATAATG TCCTGCAGTT GGAGTATGAC AAAGTGGAGA TCCTAAGGAC AGTTTTTCTG
    CGTAGCCGAA AGCGGGCCAA GGACCTAATC AGTGAGCAGC TGCGTGAGTT TCAGCAGAAG
    CGCACCGCCG GCCTGGGAAC CATTTACGGA CCCACGGACG ACAAGCTGGC CGAGGCGAAG
    ACGGATAAGC TAAGGGAGCA AATCATCGAC AAATATTTAA TGCCCAATCT TCACGCGCTT
    ATTGAGGATG AGAACGGTTC ACCGCCGGAG GATGTACGCA AGGTGGCGTT GTGTTCTGCT
    CTTTCCACCG TCATCTACCG TATTTTCAAC ACTCGTCCGC CTCCAAGCAG CATCGTTGAG
    CGGGTCCATC ACTTTGTGAG CAGGGACAAG AGTTTCAAGT CGCGTATTAT GGGCAAAAAT
    CGCAAGATGA ATGTTCGTGG TCATCCATTG GTATTGCGTC AATACTATGA AGTGACGCAC
    TGCAATCATT GTCAGACGAT TATCTGGGGC GTGAGCCCGC AAGGTTATCA TTGTACAGAC
    TGTAAATTGA ACATACACCG TCAGTGCTCG AAAGTAGTGG ACGAGAGTTG TCCCGGTCCC
    TTGCCCCAGG CAAAACGTCT CGCCCACAAC GACAAGATCA GTAAATTCAT GGGCAAAATT
    CGACCGCGTA CCAGCGACGT CATTGGAAAT GAAAAGCGAA GTCGTCAAGA TGAGGAATTG
    GATGTTGAGT TGACTCCAGA CCGTGGTCAG GCGTCGATTG TGCGTCAACC CTCTGATCGG
    CGACCGGATG CGAACATATC GATAAGGTCG AATGGAAATA CCTCCTGCAA CACTTCGGGG
    CTGAACACCA CCGACCTGCA AAGTTCTTTT CACGGCAGCT GTGCCAACGA CAGTATTAAC
    CCCGGCGGTG GAGCCGGATG CAACATGGAT TTATCCACGA GCGTGGCGTC AACGACTCCG
    TCGACCAGTG GATCCGTGGC AGCCGGTCTG AGTGCTTTTG CCGAACTGAA CGCCCTGGAT
    ACAGTGGATA AAGAAGCGCG TAGGGAGCGT TACAGTCAGC ATCCGAAGCA CAAAAGTGCA
    CCAGTCTCTG TGAATCGGTC GGAATCCTAC AAGGAGCGCT TGTCCAACAA GAGGAACCGC
    AACAGTCGCC GCAAGACCTC TGATCCAAGC TTGTCGTCGC GTCCCAATGA TGAACAACTG
    GACTTGGGTC TATCGAATGC CACTTATGTG GGCAGTTCGA ATTCTAGTCT CTCTTCAGCT
    GGCGGCAGCG AGAGTCCCAG CACGTCAATG GAGCACTTTG CTGCACCCGG AGCAGCGGGT
    GGCGTCCAGG TGCCGCCAAT GGGATTGAAC CAGAACCAGC ACCCCCATCT GCTCATCCAG
    CAGCACGCCC AGCAGTACTG CCAGCAGGAT TCCTTTCAGG CGGGTTTGGC AGGGGCCGCT
    GGGAGCAGTG CAGCTAGCAA CTCTAGTTTC TGGAATGCTG GCCACCCATT GCCTGTAGCT
    CGTTGGACGC TGGAGAGCGA GGATGAAGAC GACGTGAACG AGGCGGACTG GAGTTCCATG
    GTGGCCGCAG AGGTGTTGGC AGCCTTAACG GACGCTGAGA AGAAGCGTCA GGAGATTATA
    AATGAAATCT ATCAAACTGA ACGCAACCAT GTGCGCACCC TAAAGCTGCT GGATCGATTA
    TTCTTCCTGC CACTCTACGA GAGTGGATTG CTGTCCCAGG ATCATTTGCT GTTGTTGTTC
    CCGCCCGCCT TGCTGTCGCT CCGTGAGATT CATGGCGCCT TCGAGCAGAG TCTTAAGCAA
    CGACGCATCG AGCATAACCA CGTGGTGAAC ACCATAGGGG ATCTGCTCGC CGACATGTTC
    GATGGTCAGT CTGGAGTTGT TCTTTGCGAG TTTGCGGCTC AGTTCTGTGC CCGCCAGCAA
    ATCGCCTTGG AGGCACTCAA GGAGAAGCGC AACAAGGACG AGATGCTGCA AAAGCTATTG
    AAAAAGTCGG AGTCACACAA GGCATGTCGC CGCCTCGAGC TAAAGGACTT GCTGCCCACC
    GTGCTGCAGC GCCTCACCAA GTATCCGCTT TTGTTTGAGA ACCTTTACAA GGTGACCGTG
    CGCTTGCTGC CAGAAAACAC CACCGAGGCG GAAGCCATTC AACGGGCAGT GGAATCCTCT
    AAAAGGATTC TTGTCGAGGT CAACCAGGCA GTAAAGACAG CAGAGGATGC TCACAAGCTG
    CAAAACATTC AGCGTAAGTT AGACAGATCC TCCTATGACA AGGAAGAGTT TAAGAAATTG
    GACCTGACCC AACATCACCT CATCCATGAC GGCAATCTGA CGATCAAGAA GAATCCTAGC
    GTGCAGCTAC ATGGACTTCT GTTTGAAAAC ATGATTGTTT TGCTGACCAA GCAGGATGAT
    AAATATTATC TAAAGAACTT GCACACCCCG CTATCGATCA CCAATAAGCC AGTCAGTCCA
    ATTATGAGCA TTGATGCGGA CACTTTGATC CGGCAGGAGG CGGCTGATAA AAATTCCTTT
    TTCCTCATCA AGATGAAGAC ATCACAAATG TTGGAGCTAC GCGCGCCTAG TAGCTCGGAG
    TGCAAGACAT GGTTTAAACA CTTCTCGGAT GTGGCTGCTC GTCAGTCTAA GAATCGTTCA
    AAGAACGCAT CAAGCAACCA TGACACGAGT ATTAGTGATC CAGCTCTCGC CGCTATTCCG
    CATTCCAACA CCAAAGAGTC GTTGGAGTTG AGCACTGATA CAGTACAGCC ATTGGCTGCG
    ACAGCCACAT TGACTACCAC ACCATTGGCC CCAATGCTGC CTATAGCCAC GGTTACACCG
    GCTCCAGCGA CCAATAATAG TAACGTTAGC TCTCTTACTG GAGTTCAGTT GCGAAACCCT
    CAACGGGATG CGACAGCAAG TGAATCTGAT GCGGATTATG TAAACACACC AAAGCCGCGT
    TCGAGCCAAA ATGAAGTTAA TCGCACTATG TCCATAAGAA GCACTGGCGA ACCCATTCAG
    AAGTATTCGG CGAATGGGAC GGAAGCAAAC GACGTTACTT TACGACACTC TCAGTCGACT
    AGGGAATCGG TTAGACCAGG ATCTACTGGG GAGGAGCGAA ACTCCACGTA TGGTATGGTT
    GGAGGTAACT CCAAACGCGA CAGCGCCAGC ATTGTCTGCT CGAACAACTC GAACAACACG
    CGCACCCTTC TGATGCAGAG TCCCTTGGTG GACCCTACGG CCATTCAGGT CAGCATTAGT
    CCCGCTCACA CAGCGGAACC TGTGTTGACA CCAGGAGAGA AGTTACGCCG CTTGGACGCC
    TCCATTAGGA ATGATTTGCT GGAGAAGCAG AAAATCATTT GTGATATCTT CCGTTTGCCA
    GTGGAACACT ACGACCAGAT TGTGGACATT GCCATGATGC CAGAGGCGCC GAAAGACAGT
    GCAGATATTG CTTTAGCTGC TTACGATCAG ATTCAAACCC TGACCAAGAT GCTGAACGAG
    TACATGCACG TCACGCCTGA GCAAGAGGTC TCAGCGGTGT CCACGGCGGT TTGTGGCCAC
    TGTCACGAGA AGGAAAAGCT TCGTAAGAAG GTGGCACCAT CCTCATCGTT TTCTTCATCA
    CCACCACCGC TGCCGCCTCC CAATAGGCAG CATGCCCAGG CTCAGGCCCA GATACCGCCA
    TCGCGGCTGA TGCCCAAACT ACAAACTCTT GATCTTGACG AAGTTGCCAT ACACGAAGAC
    GATGACGGAT ACTGTGAGAT CGACGAACTG CGCTTACCGG CTATTCCGTC CAAACCACAT
    GAGCGGCCCA CAACGCCACT GGCTCCTTTC AATACTGAGC CGAAAACTTC ACAATCTGTT
    ATAGATGCCT CGAAACGTCA ATCCACTGAT GCCGTTCCGG AGGGATTACT GGAACAAGAA
    CCACTCGAAG GCGATAAGAC GGAGACCAAG GGTGAAGATA ATGAAGTGAA AACTGTGCCG
    TCAGATAAGC TAAGTGAATC ATGCAATGAA GAGAGGCAAT GTGTGGAGGC GGATATCACA
    AAGGAAGTGG CAGATCCAAC GACCTCTAAG AATGAAGCTG CAGCATCGGT GGATGAATTA
    CCAAGCCAGA GCCGGGAGAT AAAAACGGCT GAAAACGCAA GCAAATCTGT AGCTGACAAA
    AAGGAAGACA ACGAGGAAAC CATCGAAGAA GGTGTGGCAT CCACGGTCGA TAGCTCCACT
    CAAACATCAC CAACTGAATC GCCGAAAGAG ACGGATAAGT TAACTGGAGG ATCGAGCAGC
    ACCTGTGGGC CGAATCGCAT TCAGCACGCT AGTGTGCTGG AGCCGAGTGT GCCCTGCCAT
    GCACTCAGCA GCATTGTAAC AATACTGAAT GAGCAGATTT CCATGCTTTT GCCAAAAATT
    AACGAACGCG ATATGGAAAG GGAGCGATTG CGTAAAGAGA ATCAACACCT TCGCGAGCTC
    TTGAGTGCGC TGCATGATCG ACAGCGAGTT GATGAAGTAA AGGAAACTCC GTTTGATCTA
    AAGAAGCTGA TGCATGCTGA GGATGTAGAG TTTGACGATG ATATTGACGC CATTTCCAAC
    AGTTCGCTGA CGCCAACGCC TACGCCGATT CCCACAGCAT CACCAAGCGC CAGCGGCCAG
    GTAGAGACAG CGGAAGCCAT GAGGATTACT AGCACTGAGG ATGAGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286515.1
    CDS463..7833
    Translation

    Target ORF information:

    RefSeq Version NM_001299586.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
    GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC
    CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG
    ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT
    CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA
    AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT
    AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG
    GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC
    CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC
    ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG
    CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC
    CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG
    CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC
    CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG
    TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT
    ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT
    CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA
    CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC
    GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC
    AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA
    AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG
    CAACTTCATC AAGTGGGAGC TGAGCAGCAA CGGATGAGCC ACCAAGCGGA GTCGATGTCT
    CAATCGATGC ATCAGCACAC CAGCACTCCG ACCTCGCAGC AGTTCTTCCA TCCGCATCAG
    CAACAACATC GCTTTAAGGA AACTGGACCG ACGTCGAAAG GCAAGAACAA GTTCCTAATT
    TCGAGGAGTT TGATCGAGGA GGATGTGCCG CCACCACTGC CGCAGAGGAA TCCGCCCAGG
    CAGTTAAATT TGGACCTGAA GAACGGAAAC GCGTCGCCGG GTGGGTCACA TCTAGTGGCT
    CCAGTTTCCG ATCTGGATCG CGCCACAAGT CCCCAATTAA ATAGATCCCA ACAACAGCAA
    CTGCCTAGCA GCACTGACAA CAGCCCGAGC AATGCCAAGT CCAAGCGTTC CAAGATCAAA
    ACGAAGGCCC TATCGGATCC TAAGATGTCC ACTCAGATGT TCCTGCAAAT GGAATCGGCG
    AGTGCAGCTG GAGCAGCCGG AGGTTCCATC GAGGTAGATG GGGGTCCACC ACCGCTACCG
    CCTCGCTTGC CTGGCATGAT GACGGAGGAT ATGAGCCGCG GCAGCTGTCA GAATCTCGCT
    CAACCCAATT CCGTGGGCAC TGCCTTCAAC TATCCTCTGG TGTCCACAAC AACAGCGGTG
    CAGAACGATA ACCTGAACAT TGCCTTTCCT TTGTCCCAAC GACCCAACAT TGTCCAGCAG
    CTGCAGCAAT ATCAACAGCA GCAGCAGCAT CAAATGAGCG GTGGCCAGGC CACAGGCGCT
    CTGGGACAGA CTCCGAATTT GGGAAAAAAC AAGCATCGAC GCGTTGGTTC TTCACCGGAC
    AATATGCATC CACGTCATCC AGATCGGATA ACGAAAACGA CTTCGGGCTC GTGGGAGATT
    GTTGAAAAGG ATGGCGAATC CTCCCCGCCC GGAACACCGC CGCCTCCATA TCTATCCAGC
    TCCCACATGA CCGTGCTGGA AGATCCGAAT GAGAACAATC GTGGAGCAGC AGCAGCCGGA
    CCTGGAGTCT TTATCGAGTC GCATCAGTTT ACGCCGATGG CGGGAGCCTC TTCTCCGATC
    CCGATATCCT TACATTCCAG CCACATGCAT GCGGCCCAGT CGAACGATAC GCAGAAGGAG
    ATCATCTCGA TGGAGGACGA AAACTCGGAC TTGGATGAGC CCTTCATTGA CGAGAACGGA
    CCCTTTAACA ATCTAACTCG TTTGTTAGAG GCCGAGAACG TCACTTTCCT AGCCATCTTC
    CTAAACTACG TGATCTCAAA CTCGGATCCC GCGCCACTTC TGTTTTACCT GATTACTGAG
    TTGTACAAGG AGGGCACCTC CAAGGACATG CGGAAATGGG CCTACGAAAT CCACTCCACA
    TTCCTCGTGC CGCGGGCTCC ATTGTCATGG TATCGCCAAG ATGAATCGCT GGCCCGCGAG
    GTGGATAATG TCCTGCAGTT GGAGTATGAC AAAGTGGAGA TCCTAAGGAC AGTTTTTCTG
    CGTAGCCGAA AGCGGGCCAA GGACCTAATC AGTGAGCAGC TGCGTGAGTT TCAGCAGAAG
    CGCACCGCCG GCCTGGGAAC CATTTACGGA CCCACGGACG ACAAGCTGGC CGAGGCGAAG
    ACGGATAAGC TAAGGGAGCA AATCATCGAC AAATATTTAA TGCCCAATCT TCACGCGCTT
    ATTGAGGATG AGAACGGTTC ACCGCCGGAG GATGTACGCA AGGTGGCGTT GTGTTCTGCT
    CTTTCCACCG TCATCTACCG TATTTTCAAC ACTCGTCCGC CTCCAAGCAG CATCGTTGAG
    CGGGTCCATC ACTTTGTGAG CAGGGACAAG AGTTTCAAGT CGCGTATTAT GGGCAAAAAT
    CGCAAGATGA ATGTTCGTGG TCATCCATTG GTATTGCGTC AATACTATGA AGTGACGCAC
    TGCAATCATT GTCAGACGAT TATCTGGGGC GTGAGCCCGC AAGGTTATCA TTGTACAGAC
    TGTAAATTGA ACATACACCG TCAGTGCTCG AAAGTAGTGG ACGAGAGTTG TCCCGGTCCC
    TTGCCCCAGG CAAAACGTCT CGCCCACAAC GACAAGATCA GTAAATTCAT GGGCAAAATT
    CGACCGCGTA CCAGCGACGT CATTGGAAAT GAAAAGCGAA GTCGTCAAGA TGAGGAATTG
    GATGTTGAGT TGACTCCAGA CCGTGGTCAG GCGTCGATTG TGCGTCAACC CTCTGATCGG
    CGACCGGATG CGAACATATC GATAAGGTCG AATGGAAATA CCTCCTGCAA CACTTCGGGG
    CTGAACACCA CCGACCTGCA AAGTTCTTTT CACGGCAGCT GTGCCAACGA CAGTATTAAC
    CCCGGCGGTG GAGCCGGATG CAACATGGAT TTATCCACGA GCGTGGCGTC AACGACTCCG
    TCGACCAGTG GATCCGTGGC AGCCGGTCTG AGTGCTTTTG CCGAACTGAA CGCCCTGGAT
    ACAGTGGATA AAGAAGCGCG TAGGGAGCGT TACAGTCAGC ATCCGAAGCA CAAAAGTGCA
    CCAGTCTCTG TGAATCGGTC GGAATCCTAC AAGGAGCGCT TGTCCAACAA GAGGAACCGC
    AACAGTCGCC GCAAGACCTC TGATCCAAGC TTGTCGTCGC GTCCCAATGA TGAACAACTG
    GACTTGGGTC TATCGAATGC CACTTATGTG GGCAGTTCGA ATTCTAGTCT CTCTTCAGCT
    GGCGGCAGCG AGAGTCCCAG CACGTCAATG GAGCACTTTG CTGCACCCGG AGCAGCGGGT
    GGCGTCCAGG TGCCGCCAAT GGGATTGAAC CAGAACCAGC ACCCCCATCT GCTCATCCAG
    CAGCACGCCC AGCAGTACTG CCAGCAGGAT TCCTTTCAGG CGGGTTTGGC AGGGGCCGCT
    GGGAGCAGTG CAGCTAGCAA CTCTAGTTTC TGGAATGCTG GCCACCCATT GCCTGTAGCT
    CGTTGGACGC TGGAGAGCGA GGATGAAGAC GACGTGAACG AGGCGGACTG GAGTTCCATG
    GTGGCCGCAG AGGTGTTGGC AGCCTTAACG GACGCTGAGA AGAAGCGTCA GGAGATTATA
    AATGAAATCT ATCAAACTGA ACGCAACCAT GTGCGCACCC TAAAGCTGCT GGATCGATTA
    TTCTTCCTGC CACTCTACGA GAGTGGATTG CTGTCCCAGG ATCATTTGCT GTTGTTGTTC
    CCGCCCGCCT TGCTGTCGCT CCGTGAGATT CATGGCGCCT TCGAGCAGAG TCTTAAGCAA
    CGACGCATCG AGCATAACCA CGTGGTGAAC ACCATAGGGG ATCTGCTCGC CGACATGTTC
    GATGGTCAGT CTGGAGTTGT TCTTTGCGAG TTTGCGGCTC AGTTCTGTGC CCGCCAGCAA
    ATCGCCTTGG AGGCACTCAA GGAGAAGCGC AACAAGGACG AGATGCTGCA AAAGCTATTG
    AAAAAGTCGG AGTCACACAA GGCATGTCGC CGCCTCGAGC TAAAGGACTT GCTGCCCACC
    GTGCTGCAGC GCCTCACCAA GTATCCGCTT TTGTTTGAGA ACCTTTACAA GGTGACCGTG
    CGCTTGCTGC CAGAAAACAC CACCGAGGCG GAAGCCATTC AACGGGCAGT GGAATCCTCT
    AAAAGGATTC TTGTCGAGGT CAACCAGGCA GTAAAGACAG CAGAGGATGC TCACAAGCTG
    CAAAACATTC AGCGTAAGTT AGACAGATCC TCCTATGACA AGGAAGAGTT TAAGAAATTG
    GACCTGACCC AACATCACCT CATCCATGAC GGCAATCTGA CGATCAAGAA GAATCCTAGC
    GTGCAGCTAC ATGGACTTCT GTTTGAAAAC ATGATTGTTT TGCTGACCAA GCAGGATGAT
    AAATATTATC TAAAGAACTT GCACACCCCG CTATCGATCA CCAATAAGCC AGTCAGTCCA
    ATTATGAGCA TTGATGCGGA CACTTTGATC CGGCAGGAGG CGGCTGATAA AAATTCCTTT
    TTCCTCATCA AGATGAAGAC ATCACAAATG TTGGAGCTAC GCGCGCCTAG TAGCTCGGAG
    TGCAAGACAT GGTTTAAACA CTTCTCGGAT GTGGCTGCTC GTCAGTCTAA GAATCGTTCA
    AAGAACGCAT CAAGCAACCA TGACACGAGT ATTAGTGATC CAGCTCTCGC CGCTATTCCG
    CATTCCAACA CCAAAGAGTC GTTGGAGTTG AGCACTGATA CAGTACAGCC ATTGGCTGCG
    ACAGCCACAT TGACTACCAC ACCATTGGCC CCAATGCTGC CTATAGCCAC GGTTACACCG
    GCTCCAGCGA CCAATAATAG TAACGTTAGC TCTCTTACTG GAGTTCAGTT GCGAAACCCT
    CAACGGGATG CGACAGCAAG TGAATCTGAT GCGGATTATG TAAACACACC AAAGCCGCGT
    TCGAGCCAAA ATGAAGTTAA TCGCACTATG TCCATAAGAA GCACTGGCGA ACCCATTCAG
    AAGTATTCGG CGAATGGGAC GGAAGCAAAC GACGTTACTT TACGACACTC TCAGTCGACT
    AGGGAATCGG TTAGACCAGG ATCTACTGGG GAGGAGCGAA ACTCCACGTA TGGTATGGTT
    GGAGGTAACT CCAAACGCGA CAGCGCCAGC ATTGTCTGCT CGAACAACTC GAACAACACG
    CGCACCCTTC TGATGCAGAG TCCCTTGGTG GACCCTACGG CCATTCAGGT CAGCATTAGT
    CCCGCTCACA CAGCGGAACC TGTGTTGACA CCAGGAGAGA AGTTACGCCG CTTGGACGCC
    TCCATTAGGA ATGATTTGCT GGAGAAGCAG AAAATCATTT GTGATATCTT CCGTTTGCCA
    GTGGAACACT ACGACCAGAT TGTGGACATT GCCATGATGC CAGAGGCGCC GAAAGACAGT
    GCAGATATTG CTTTAGCTGC TTACGATCAG ATTCAAACCC TGACCAAGAT GCTGAACGAG
    TACATGCACG TCACGCCTGA GCAAGAGGTC TCAGCGGTGT CCACGGCGGT TTGTGGCCAC
    TGTCACGAGA AGGAAAAGCT TCGTAAGAAG GTGGCACCAT CCTCATCGTT TTCTTCATCA
    CCACCACCGC TGCCGCCTCC CAATAGGCAG CATGCCCAGG CTCAGGCCCA GATACCGCCA
    TCGCGGCTGA TGCCCAAACT ACAAACTCTT GATCTTGACG AAGTTGCCAT ACACGAAGAC
    GATGACGGAT ACTGTGAGAT CGACGAACTG CGCTTACCGG CTATTCCGTC CAAACCACAT
    GAGCGGCCCA CAACGCCACT GGCTCCTTTC AATACTGAGC CGAAAACTTC ACAATCTGTT
    ATAGATGCCT CGAAACGTCA ATCCACTGAT GCCGTTCCGG AGGGATTACT GGAACAAGAA
    CCACTCGAAG GCGATAAGAC GGAGACCAAG GGTGAAGATA ATGAAGTGAA AACTGTGCCG
    TCAGATAAGC TAAGTGAATC ATGCAATGAA GAGAGGCAAT GTGTGGAGGC GGATATCACA
    AAGGAAGTGG CAGATCCAAC GACCTCTAAG AATGAAGCTG CAGCATCGGT GGATGAATTA
    CCAAGCCAGA GCCGGGAGAT AAAAACGGCT GAAAACGCAA GCAAATCTGT AGCTGACAAA
    AAGGAAGACA ACGAGGAAAC CATCGAAGAA GGTGTGGCAT CCACGGTCGA TAGCTCCACT
    CAAACATCAC CAACTGAATC GCCGAAAGAG ACGGATAAGT TAACTGGAGG ATCGAGCAGC
    ACCTGTGGGC CGAATCGCAT TCAGCACGCT AGTGTGCTGG AGCCGAGTGT GCCCTGCCAT
    GCACTCAGCA GCATTGTAAC AATACTGAAT GAGCAGATTT CCATGCTTTT GCCAAAAATT
    AACGAACGCG ATATGGAAAG GGAGCGATTG CGTAAAGAGA ATCAACACCT TCGCGAGCTC
    TTGAGTGCGC TGCATGATCG ACAGCGAGTT GATGAAGTAA AGGAAACTCC GTTTGATCTA
    AAGAAGCTGA TGCATGCTGA GGATGTAGAG TTTGACGATG ATATTGACGC CATTTCCAAC
    AGTTCGCTGA CGCCAACGCC TACGCCGATT CCCACAGCAT CACCAAGCGC CAGCGGCCAG
    GTAGAGACAG CGGAAGCCAT GAGGATTACT AGCACTGAGG ATGAGGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.