RhoGEF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGEF2
Entrez Gene ID	36915
Full Name	Rho guanine nucleotide exchange factor 2
Synonyms	CG9635,DRhoGEF,DRhoGEF2,Dmel\CG9635,DrhoGEF2,Gef2,RG2,Rho-GEF2,RhoGEF,RhoGF2,RhoGef2,Su(Rho1)2B,T2,dRhoGEF,dRhoGEF2,l(2)04291,rhoGEF2,rhogef2,shar
General protein information	Preferred Names Rho guanine nucleotide exchange factor 2 Names CG9635 gene product from transcript CG9635-RF CG9635-PD CG9635-PE CG9635-PF CG9635-PG CG9635-PH RhoGEF2-PD RhoGEF2-PE RhoGEF2-PF RhoGEF2-PG RhoGEF2-PH akkordion guanine nucleotide exchange factor 2 shar pei transcription unit 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53E4-53F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206147.4	NP_995869.1	Rho guanine nucleotide exchange factor 2, isoform E
NM_206146.4	NP_995868.1	Rho guanine nucleotide exchange factor 2, isoform F
NM_057969.6	NP_477317.1	Rho guanine nucleotide exchange factor 2, isoform D
NM_001259452.2	NP_001246381.1	Rho guanine nucleotide exchange factor 2, isoform G
NM_001299586.1	NP_001286515.1	Rho guanine nucleotide exchange factor 2, isoform H

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RhoGEF2	NP_995869.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010767	XP_318541.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.

Overexpression of DRhoGEF2, and it's functional mammalian ortholog PDZ-RhoGEF (ArhGEF11), at various stages of eye development, resulted in both dPKB/Akt-dependent and -independent phenotypes in Drosophila
Title: The Rho Guanine Nucleotide Exchange Factor DRhoGEF2 Is a Genetic Modifier of the PI3K Pathway in Drosophila.

RhoGEF2 also cooperates with an activated allele of a downstream effector of Ras, Raf (Raf(GOF)).
Title: In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.

Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Title: The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.

early Drosophila embryogenesis is primed to form furrows at either overlapping astral microtubules or the central spindle; the shift to the latter is driven by a corresponding shift from RhoGEF2 to Pebble in controlling furrow formation.
Title: RhoGEF and positioning of rappaport-like furrows in the early Drosophila embryo.

The following RhoGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10304	NM_206146.4 Latest version!	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa10304	NM_057969.6 Latest version!	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa10305	NM_206147.4 Latest version!	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa12368	NM_001259452.2 Latest version!	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa21838	NM_001299586.1 Latest version!	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGEF2 ( NM_206146.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995868.1 Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa10304
Clone ID Related Accession (Same CDS sequence)	NM_206146.4 , NM_057969.6
Accession Version	NM_206146.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho guanine nucleotide exchange factor 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206146.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC 
AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG 
AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT 
GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC 
TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA 
GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG 
CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA 
GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG 
CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG 
AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG 
GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG 
GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG 
TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT 
CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC 
GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC 
CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA 
AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG 
CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG 
GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG 
GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT 
CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT 
AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG 
AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG 
CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA 
GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA 
AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT 
AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG 
AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC 
GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA 
TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG 
GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG 
CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA 
AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA 
CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT 
CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT 
GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG 
AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC 
AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC 
TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC 
CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG 
AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG 
CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT 
TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT 
TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG 
ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA 
CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC 
AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA 
CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG 
GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA 
ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG 
GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG 
GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA 
GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT 
AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA 
TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG 
CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG 
CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT 
CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG 
TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC 
ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC 
AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995868.1
CDS	474..8153
Translation	MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSVPELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVEFDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEE

Target ORF information:

RefSeq Version	NM_206146.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206146.4

ORF Insert Sequence:

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC 
AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG 
AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT 
GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC 
TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA 
GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG 
CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA 
GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG 
CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG 
AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG 
GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG 
GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG 
TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT 
CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC 
GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC 
CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA 
AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG 
CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG 
GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG 
GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT 
CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT 
AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG 
AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG 
CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA 
GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA 
AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT 
AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG 
AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC 
GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA 
TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG 
GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG 
CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA 
AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA 
CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT 
CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT 
GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG 
AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC 
AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC 
TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC 
CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG 
AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG 
CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT 
TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT 
TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG 
ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA 
CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC 
AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA 
CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG 
GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA 
ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG 
GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG 
GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA 
GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT 
AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA 
TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG 
CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG 
CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT 
CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG 
TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC 
ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC 
AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGEF2 ( NM_057969.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477317.1 Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant D, mRNA.

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CloneID	OFa10304
Clone ID Related Accession (Same CDS sequence)	NM_206146.4 , NM_057969.6
Accession Version	NM_057969.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho guanine nucleotide exchange factor 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057969.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC 
AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG 
AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT 
GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC 
TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA 
GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG 
CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA 
GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG 
CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG 
AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG 
GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG 
GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG 
TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT 
CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC 
GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC 
CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA 
AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG 
CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG 
GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG 
GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT 
CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT 
AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG 
AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG 
CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA 
GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA 
AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT 
AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG 
AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC 
GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA 
TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG 
GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG 
CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA 
AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA 
CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT 
CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT 
GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG 
AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC 
AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC 
TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC 
CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG 
AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG 
CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT 
TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT 
TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG 
ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA 
CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC 
AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA 
CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG 
GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA 
ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG 
GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG 
GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA 
GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT 
AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA 
TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG 
CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG 
CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT 
CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG 
TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC 
ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC 
AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477317.1
CDS	463..8142
Translation	MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSVPELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVEFDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEE

Target ORF information:

RefSeq Version	NM_057969.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057969.6

ORF Insert Sequence:

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTT ACAGTCAGCA TCCGAAGCAC 
AAAAGTGCAC CAGTCTCTGT GAATCGGTCG GAATCCTACA AGGAGCGCTT GTCCAACAAG 
AGGAACCGCA ACAGTCGCCG CAAGACCTCT GATCCAAGCT TGTCGTCGCG TCCCAATGAT 
GAACAACTGG ACTTGGGTCT ATCGAATGCC ACTTATGTGG GCAGTTCGAA TTCTAGTCTC 
TCTTCAGCTG GCGGCAGCGA GAGTCCCAGC ACGTCAATGG AGCACTTTGC TGCACCCGGA 
GCAGCGGGTG GCGTCCAGGT GCCGCCAATG GGATTGAACC AGAACCAGCA CCCCCATCTG 
CTCATCCAGC AGCACGCCCA GCAGTACTGC CAGCAGGATT CCTTTCAGGC GGGTTTGGCA 
GGGGCCGCTG GGAGCAGTGC AGCTAGCAAC TCTAGTTTCT GGAATGCTGG CCACCCATTG 
CCTGTAGCTC GTTGGACGCT GGAGAGCGAG GATGAAGACG ACGTGAACGA GGCGGACTGG 
AGTTCCATGG TGGCCGCAGA GGTGTTGGCA GCCTTAACGG ACGCTGAGAA GAAGCGTCAG 
GAGATTATAA ATGAAATCTA TCAAACTGAA CGCAACCATG TGCGCACCCT AAAGCTGCTG 
GATCGATTAT TCTTCCTGCC ACTCTACGAG AGTGGATTGC TGTCCCAGGA TCATTTGCTG 
TTGTTGTTCC CGCCCGCCTT GCTGTCGCTC CGTGAGATTC ATGGCGCCTT CGAGCAGAGT 
CTTAAGCAAC GACGCATCGA GCATAACCAC GTGGTGAACA CCATAGGGGA TCTGCTCGCC 
GACATGTTCG ATGGTCAGTC TGGAGTTGTT CTTTGCGAGT TTGCGGCTCA GTTCTGTGCC 
CGCCAGCAAA TCGCCTTGGA GGCACTCAAG GAGAAGCGCA ACAAGGACGA GATGCTGCAA 
AAGCTATTGA AAAAGTCGGA GTCACACAAG GCATGTCGCC GCCTCGAGCT AAAGGACTTG 
CTGCCCACCG TGCTGCAGCG CCTCACCAAG TATCCGCTTT TGTTTGAGAA CCTTTACAAG 
GTGACCGTGC GCTTGCTGCC AGAAAACACC ACCGAGGCGG AAGCCATTCA ACGGGCAGTG 
GAATCCTCTA AAAGGATTCT TGTCGAGGTC AACCAGGCAG TAAAGACAGC AGAGGATGCT 
CACAAGCTGC AAAACATTCA GCGTAAGTTA GACAGATCCT CCTATGACAA GGAAGAGTTT 
AAGAAATTGG ACCTGACCCA ACATCACCTC ATCCATGACG GCAATCTGAC GATCAAGAAG 
AATCCTAGCG TGCAGCTACA TGGACTTCTG TTTGAAAACA TGATTGTTTT GCTGACCAAG 
CAGGATGATA AATATTATCT AAAGAACTTG CACACCCCGC TATCGATCAC CAATAAGCCA 
GTCAGTCCAA TTATGAGCAT TGATGCGGAC ACTTTGATCC GGCAGGAGGC GGCTGATAAA 
AATTCCTTTT TCCTCATCAA GATGAAGACA TCACAAATGT TGGAGCTACG CGCGCCTAGT 
AGCTCGGAGT GCAAGACATG GTTTAAACAC TTCTCGGATG TGGCTGCTCG TCAGTCTAAG 
AATCGTTCAA AGAACGCATC AAGCAACCAT GACACGAGTA TTAGTGATCC AGCTCTCGCC 
GCTATTCCGC ATTCCAACAC CAAAGAGTCG TTGGAGTTGA GCACTGATAC AGTACAGCCA 
TTGGCTGCGA CAGCCACATT GACTACCACA CCATTGGCCC CAATGCTGCC TATAGCCACG 
GTTACACCGG CTCCAGCGAC CAATAATAGT AACGTTAGCT CTCTTACTGG AGTTCAGTTG 
CGAAACCCTC AACGGGATGC GACAGCAAGT GAATCTGATG CGGATTATGT AAACACACCA 
AAGCCGCGTT CGAGCCAAAA TGAAGTTAAT CGCACTATGT CCATAAGAAG CACTGGCGAA 
CCCATTCAGA AGTATTCGGC GAATGGGACG GAAGCAAACG ACGTTACTTT ACGACACTCT 
CAGTCGACTA GGGAATCGGT TAGACCAGGA TCTACTGGGG AGGAGCGAAA CTCCACGTAT 
GGTATGGTTG GAGGTAACTC CAAACGCGAC AGCGCCAGCA TTGTCTGCTC GAACAACTCG 
AACAACACGC GCACCCTTCT GATGCAGAGT CCCTTGGTGG ACCCTACGGC CATTCAGGTC 
AGCATTAGTC CCGCTCACAC AGCGGAACCT GTGTTGACAC CAGGAGAGAA GTTACGCCGC 
TTGGACGCCT CCATTAGGAA TGATTTGCTG GAGAAGCAGA AAATCATTTG TGATATCTTC 
CGTTTGCCAG TGGAACACTA CGACCAGATT GTGGACATTG CCATGATGCC AGAGGCGCCG 
AAAGACAGTG CAGATATTGC TTTAGCTGCT TACGATCAGA TTCAAACCCT GACCAAGATG 
CTGAACGAGT ACATGCACGT CACGCCTGAG CAAGAGGTCT CAGCGGTGTC CACGGCGGTT 
TGTGGCCACT GTCACGAGAA GGAAAAGCTT CGTAAGAAGG TGGCACCATC CTCATCGTTT 
TCTTCATCAC CACCACCGCT GCCGCCTCCC AATAGGCAGC ATGCCCAGGC TCAGGCCCAG 
ATACCGCCAT CGCGGCTGAT GCCCAAACTA CAAACTCTTG ATCTTGACGA AGTTGCCATA 
CACGAAGACG ATGACGGATA CTGTGAGATC GACGAACTGC GCTTACCGGC TATTCCGTCC 
AAACCACATG AGCGGCCCAC AACGCCACTG GCTCCTTTCA ATACTGAGCC GAAAACTTCA 
CAATCTGTTA TAGATGCCTC GAAACGTCAA TCCACTGATG CCGTTCCGGA GGGATTACTG 
GAACAAGAAC CACTCGAAGG CGATAAGACG GAGACCAAGG GTGAAGATAA TGAAGTGAAA 
ACTGTGCCGT CAGATAAGCT AAGTGAATCA TGCAATGAAG AGAGGCAATG TGTGGAGGCG 
GATATCACAA AGGAAGTGGC AGATCCAACG ACCTCTAAGA ATGAAGCTGC AGCATCGGTG 
GATGAATTAC CAAGCCAGAG CCGGGAGATA AAAACGGCTG AAAACGCAAG CAAATCTGTA 
GCTGACAAAA AGGAAGACAA CGAGGAAACC ATCGAAGAAG GTGTGGCATC CACGGTCGAT 
AGCTCCACTC AAACATCACC AACTGAATCG CCGAAAGAGA CGGATAAGTT AACTGGAGGA 
TCGAGCAGCA CCTGTGGGCC GAATCGCATT CAGCACGCTA GTGTGCTGGA GCCGAGTGTG 
CCCTGCCATG CACTCAGCAG CATTGTAACA ATACTGAATG AGCAGATTTC CATGCTTTTG 
CCAAAAATTA ACGAACGCGA TATGGAAAGG GAGCGATTGC GTAAAGAGAA TCAACACCTT 
CGCGAGCTCT TGAGTGCGCT GCATGATCGA CAGCGAGTTG ATGAAGTAAA GGAAACTCCG 
TTTGATCTAA AGAAGCTGAT GCATGCTGAG GATGTAGAGT TTGACGATGA TATTGACGCC 
ATTTCCAACA GTTCGCTGAC GCCAACGCCT ACGCCGATTC CCACAGCATC ACCAAGCGCC 
AGCGGCCAGG TAGAGACAGC GGAAGCCATG AGGATTACTA GCACTGAGGA TGAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGEF2 ( NM_206147.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995869.1 Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant E, mRNA.

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CloneID	OFa10305
Clone ID Related Accession (Same CDS sequence)	NM_206147.4
Accession Version	NM_206147.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho guanine nucleotide exchange factor 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206147.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGCTCTG GGACAGACTC CGAATTTGGG AAAAAACAAG 
CATCGACGCG TTGGTTCTTC ACCGGACAAT ATGCATCCAC GTCATCCAGA TCGGATAACG 
AAAACGACTT CGGGCTCGTG GGAGATTGTT GAAAAGGATG GCGAATCCTC CCCGCCCGGA 
ACACCGCCGC CTCCATATCT ATCCAGCTCC CACATGACCG TGCTGGAAGA TCCGAATGAG 
AACAATCGTG GAGCAGCAGC AGCCGGACCT GGAGTCTTTA TCGAGTCGCA TCAGTTTACG 
CCGATGGCGG GAGCCTCTTC TCCGATCCCG ATATCCTTAC ATTCCAGCCA CATGCATGCG 
GCCCAGTCGA ACGATACGCA GAAGGAGATC ATCTCGATGG AGGACGAAAA CTCGGACTTG 
GATGAGCCCT TCATTGACGA GAACGGACCC TTTAACAATC TAACTCGTTT GTTAGAGGCC 
GAGAACGTCA CTTTCCTAGC CATCTTCCTA AACTACGTGA TCTCAAACTC GGATCCCGCG 
CCACTTCTGT TTTACCTGAT TACTGAGTTG TACAAGGAGG GCACCTCCAA GGACATGCGG 
AAATGGGCCT ACGAAATCCA CTCCACATTC CTCGTGCCGC GGGCTCCATT GTCATGGTAT 
CGCCAAGATG AATCGCTGGC CCGCGAGGTG GATAATGTCC TGCAGTTGGA GTATGACAAA 
GTGGAGATCC TAAGGACAGT TTTTCTGCGT AGCCGAAAGC GGGCCAAGGA CCTAATCAGT 
GAGCAGCTGC GTGAGTTTCA GCAGAAGCGC ACCGCCGGCC TGGGAACCAT TTACGGACCC 
ACGGACGACA AGCTGGCCGA GGCGAAGACG GATAAGCTAA GGGAGCAAAT CATCGACAAA 
TATTTAATGC CCAATCTTCA CGCGCTTATT GAGGATGAGA ACGGTTCACC GCCGGAGGAT 
GTACGCAAGG TGGCGTTGTG TTCTGCTCTT TCCACCGTCA TCTACCGTAT TTTCAACACT 
CGTCCGCCTC CAAGCAGCAT CGTTGAGCGG GTCCATCACT TTGTGAGCAG GGACAAGAGT 
TTCAAGTCGC GTATTATGGG CAAAAATCGC AAGATGAATG TTCGTGGTCA TCCATTGGTA 
TTGCGTCAAT ACTATGAAGT GACGCACTGC AATCATTGTC AGACGATTAT CTGGGGCGTG 
AGCCCGCAAG GTTATCATTG TACAGACTGT AAATTGAACA TACACCGTCA GTGCTCGAAA 
GTAGTGGACG AGAGTTGTCC CGGTCCCTTG CCCCAGGCAA AACGTCTCGC CCACAACGAC 
AAGATCAGTA AATTCATGGG CAAAATTCGA CCGCGTACCA GCGACGTCAT TGGAAATGAA 
AAGCGAAGTC GTCAAGATGA GGAATTGGAT GTTGAGTTGA CTCCAGACCG TGGTCAGGCG 
TCGATTGTGC GTCAACCCTC TGATCGGCGA CCGGATGCGA ACATATCGAT AAGGTCGAAT 
GGAAATACCT CCTGCAACAC TTCGGGGCTG AACACCACCG ACCTGCAAAG TTCTTTTCAC 
GGCAGCTGTG CCAACGACAG TATTAACCCC GGCGGTGGAG CCGGATGCAA CATGGATTTA 
TCCACGAGCG TGGCGTCAAC GACTCCGTCG ACCAGTGGAT CCGTGGCAGC CGGTCTGAGT 
GCTTTTGCCG AACTGAACGC CCTGGATACA GTGGATAAAG AAGCGCGTAG GGAGCGTTAC 
AGTCAGCATC CGAAGCACAA AAGTGCACCA GTCTCTGTGA ATCGGTCGGA ATCCTACAAG 
GAGCGCTTGT CCAACAAGAG GAACCGCAAC AGTCGCCGCA AGACCTCTGA TCCAAGCTTG 
TCGTCGCGTC CCAATGATGA ACAACTGGAC TTGGGTCTAT CGAATGCCAC TTATGTGGGC 
AGTTCGAATT CTAGTCTCTC TTCAGCTGGC GGCAGCGAGA GTCCCAGCAC GTCAATGGAG 
CACTTTGCTG CACCCGGAGC AGCGGGTGGC GTCCAGGTGC CGCCAATGGG ATTGAACCAG 
AACCAGCACC CCCATCTGCT CATCCAGCAG CACGCCCAGC AGTACTGCCA GCAGGATTCC 
TTTCAGGCGG GTTTGGCAGG GGCCGCTGGG AGCAGTGCAG CTAGCAACTC TAGTTTCTGG 
AATGCTGGCC ACCCATTGCC TGTAGCTCGT TGGACGCTGG AGAGCGAGGA TGAAGACGAC 
GTGAACGAGG CGGACTGGAG TTCCATGGTG GCCGCAGAGG TGTTGGCAGC CTTAACGGAC 
GCTGAGAAGA AGCGTCAGGA GATTATAAAT GAAATCTATC AAACTGAACG CAACCATGTG 
CGCACCCTAA AGCTGCTGGA TCGATTATTC TTCCTGCCAC TCTACGAGAG TGGATTGCTG 
TCCCAGGATC ATTTGCTGTT GTTGTTCCCG CCCGCCTTGC TGTCGCTCCG TGAGATTCAT 
GGCGCCTTCG AGCAGAGTCT TAAGCAACGA CGCATCGAGC ATAACCACGT GGTGAACACC 
ATAGGGGATC TGCTCGCCGA CATGTTCGAT GGTCAGTCTG GAGTTGTTCT TTGCGAGTTT 
GCGGCTCAGT TCTGTGCCCG CCAGCAAATC GCCTTGGAGG CACTCAAGGA GAAGCGCAAC 
AAGGACGAGA TGCTGCAAAA GCTATTGAAA AAGTCGGAGT CACACAAGGC ATGTCGCCGC 
CTCGAGCTAA AGGACTTGCT GCCCACCGTG CTGCAGCGCC TCACCAAGTA TCCGCTTTTG 
TTTGAGAACC TTTACAAGGT GACCGTGCGC TTGCTGCCAG AAAACACCAC CGAGGCGGAA 
GCCATTCAAC GGGCAGTGGA ATCCTCTAAA AGGATTCTTG TCGAGGTCAA CCAGGCAGTA 
AAGACAGCAG AGGATGCTCA CAAGCTGCAA AACATTCAGC GTAAGTTAGA CAGATCCTCC 
TATGACAAGG AAGAGTTTAA GAAATTGGAC CTGACCCAAC ATCACCTCAT CCATGACGGC 
AATCTGACGA TCAAGAAGAA TCCTAGCGTG CAGCTACATG GACTTCTGTT TGAAAACATG 
ATTGTTTTGC TGACCAAGCA GGATGATAAA TATTATCTAA AGAACTTGCA CACCCCGCTA 
TCGATCACCA ATAAGCCAGT CAGTCCAATT ATGAGCATTG ATGCGGACAC TTTGATCCGG 
CAGGAGGCGG CTGATAAAAA TTCCTTTTTC CTCATCAAGA TGAAGACATC ACAAATGTTG 
GAGCTACGCG CGCCTAGTAG CTCGGAGTGC AAGACATGGT TTAAACACTT CTCGGATGTG 
GCTGCTCGTC AGTCTAAGAA TCGTTCAAAG AACGCATCAA GCAACCATGA CACGAGTATT 
AGTGATCCAG CTCTCGCCGC TATTCCGCAT TCCAACACCA AAGAGTCGTT GGAGTTGAGC 
ACTGATACAG TACAGCCATT GGCTGCGACA GCCACATTGA CTACCACACC ATTGGCCCCA 
ATGCTGCCTA TAGCCACGGT TACACCGGCT CCAGCGACCA ATAATAGTAA CGTTAGCTCT 
CTTACTGGAG TTCAGTTGCG AAACCCTCAA CGGGATGCGA CAGCAAGTGA ATCTGATGCG 
GATTATGTAA ACACACCAAA GCCGCGTTCG AGCCAAAATG AAGTTAATCG CACTATGTCC 
ATAAGAAGCA CTGGCGAACC CATTCAGAAG TATTCGGCGA ATGGGACGGA AGCAAACGAC 
GTTACTTTAC GACACTCTCA GTCGACTAGG GAATCGGTTA GACCAGGATC TACTGGGGAG 
GAGCGAAACT CCACGTATGG TATGGTTGGA GGTAACTCCA AACGCGACAG CGCCAGCATT 
GTCTGCTCGA ACAACTCGAA CAACACGCGC ACCCTTCTGA TGCAGAGTCC CTTGGTGGAC 
CCTACGGCCA TTCAGGTCAG CATTAGTCCC GCTCACACAG CGGAACCTGT GTTGACACCA 
GGAGAGAAGT TACGCCGCTT GGACGCCTCC ATTAGGAATG ATTTGCTGGA GAAGCAGAAA 
ATCATTTGTG ATATCTTCCG TTTGCCAGTG GAACACTACG ACCAGATTGT GGACATTGCC 
ATGATGCCAG AGGCGCCGAA AGACAGTGCA GATATTGCTT TAGCTGCTTA CGATCAGATT 
CAAACCCTGA CCAAGATGCT GAACGAGTAC ATGCACGTCA CGCCTGAGCA AGAGGTCTCA 
GCGGTGTCCA CGGCGGTTTG TGGCCACTGT CACGAGAAGG AAAAGCTTCG TAAGAAGGTG 
GCACCATCCT CATCGTTTTC TTCATCACCA CCACCGCTGC CGCCTCCCAA TAGGCAGCAT 
GCCCAGGCTC AGGCCCAGAT ACCGCCATCG CGGCTGATGC CCAAACTACA AACTCTTGAT 
CTTGACGAAG TTGCCATACA CGAAGACGAT GACGGATACT GTGAGATCGA CGAACTGCGC 
TTACCGGCTA TTCCGTCCAA ACCACATGAG CGGCCCACAA CGCCACTGGC TCCTTTCAAT 
ACTGAGCCGA AAACTTCACA ATCTGTTATA GATGCCTCGA AACGTCAATC CACTGATGCC 
GTTCCGGAGG GATTACTGGA ACAAGAACCA CTCGAAGGCG ATAAGACGGA GACCAAGGGT 
GAAGATAATG AAGTGAAAAC TGTGCCGTCA GATAAGCTAA GTGAATCATG CAATGAAGAG 
AGGCAATGTG TGGAGGCGGA TATCACAAAG GAAGTGGCAG ATCCAACGAC CTCTAAGAAT 
GAAGCTGCAG CATCGGTGGA TGAATTACCA AGCCAGAGCC GGGAGATAAA AACGGCTGAA 
AACGCAAGCA AATCTGTAGC TGACAAAAAG GAAGACAACG AGGAAACCAT CGAAGAAGGT 
GTGGCATCCA CGGTCGATAG CTCCACTCAA ACATCACCAA CTGAATCGCC GAAAGAGACG 
GATAAGTTAA CTGGAGGATC GAGCAGCACC TGTGGGCCGA ATCGCATTCA GCACGCTAGT 
GTGCTGGAGC CGAGTGTGCC CTGCCATGCA CTCAGCAGCA TTGTAACAAT ACTGAATGAG 
CAGATTTCCA TGCTTTTGCC AAAAATTAAC GAACGCGATA TGGAAAGGGA GCGATTGCGT 
AAAGAGAATC AACACCTTCG CGAGCTCTTG AGTGCGCTGC ATGATCGACA GCGAGTTGAT 
GAAGTAAAGG AAACTCCGTT TGATCTAAAG AAGCTGATGC ATGCTGAGGA TGTAGAGTTT 
GACGATGATA TTGACGCCAT TTCCAACAGT TCGCTGACGC CAACGCCTAC GCCGATTCCC 
ACAGCATCAC CAAGCGCCAG CGGCCAGGTA GAGACAGCGG AAGCCATGAG GATTACTAGC 
ACTGAGGATG AGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995869.1
CDS	474..7091
Translation	MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSVPELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVEFDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEE

Target ORF information:

RefSeq Version	NM_206147.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206147.4

ORF Insert Sequence:

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGCTCTG GGACAGACTC CGAATTTGGG AAAAAACAAG 
CATCGACGCG TTGGTTCTTC ACCGGACAAT ATGCATCCAC GTCATCCAGA TCGGATAACG 
AAAACGACTT CGGGCTCGTG GGAGATTGTT GAAAAGGATG GCGAATCCTC CCCGCCCGGA 
ACACCGCCGC CTCCATATCT ATCCAGCTCC CACATGACCG TGCTGGAAGA TCCGAATGAG 
AACAATCGTG GAGCAGCAGC AGCCGGACCT GGAGTCTTTA TCGAGTCGCA TCAGTTTACG 
CCGATGGCGG GAGCCTCTTC TCCGATCCCG ATATCCTTAC ATTCCAGCCA CATGCATGCG 
GCCCAGTCGA ACGATACGCA GAAGGAGATC ATCTCGATGG AGGACGAAAA CTCGGACTTG 
GATGAGCCCT TCATTGACGA GAACGGACCC TTTAACAATC TAACTCGTTT GTTAGAGGCC 
GAGAACGTCA CTTTCCTAGC CATCTTCCTA AACTACGTGA TCTCAAACTC GGATCCCGCG 
CCACTTCTGT TTTACCTGAT TACTGAGTTG TACAAGGAGG GCACCTCCAA GGACATGCGG 
AAATGGGCCT ACGAAATCCA CTCCACATTC CTCGTGCCGC GGGCTCCATT GTCATGGTAT 
CGCCAAGATG AATCGCTGGC CCGCGAGGTG GATAATGTCC TGCAGTTGGA GTATGACAAA 
GTGGAGATCC TAAGGACAGT TTTTCTGCGT AGCCGAAAGC GGGCCAAGGA CCTAATCAGT 
GAGCAGCTGC GTGAGTTTCA GCAGAAGCGC ACCGCCGGCC TGGGAACCAT TTACGGACCC 
ACGGACGACA AGCTGGCCGA GGCGAAGACG GATAAGCTAA GGGAGCAAAT CATCGACAAA 
TATTTAATGC CCAATCTTCA CGCGCTTATT GAGGATGAGA ACGGTTCACC GCCGGAGGAT 
GTACGCAAGG TGGCGTTGTG TTCTGCTCTT TCCACCGTCA TCTACCGTAT TTTCAACACT 
CGTCCGCCTC CAAGCAGCAT CGTTGAGCGG GTCCATCACT TTGTGAGCAG GGACAAGAGT 
TTCAAGTCGC GTATTATGGG CAAAAATCGC AAGATGAATG TTCGTGGTCA TCCATTGGTA 
TTGCGTCAAT ACTATGAAGT GACGCACTGC AATCATTGTC AGACGATTAT CTGGGGCGTG 
AGCCCGCAAG GTTATCATTG TACAGACTGT AAATTGAACA TACACCGTCA GTGCTCGAAA 
GTAGTGGACG AGAGTTGTCC CGGTCCCTTG CCCCAGGCAA AACGTCTCGC CCACAACGAC 
AAGATCAGTA AATTCATGGG CAAAATTCGA CCGCGTACCA GCGACGTCAT TGGAAATGAA 
AAGCGAAGTC GTCAAGATGA GGAATTGGAT GTTGAGTTGA CTCCAGACCG TGGTCAGGCG 
TCGATTGTGC GTCAACCCTC TGATCGGCGA CCGGATGCGA ACATATCGAT AAGGTCGAAT 
GGAAATACCT CCTGCAACAC TTCGGGGCTG AACACCACCG ACCTGCAAAG TTCTTTTCAC 
GGCAGCTGTG CCAACGACAG TATTAACCCC GGCGGTGGAG CCGGATGCAA CATGGATTTA 
TCCACGAGCG TGGCGTCAAC GACTCCGTCG ACCAGTGGAT CCGTGGCAGC CGGTCTGAGT 
GCTTTTGCCG AACTGAACGC CCTGGATACA GTGGATAAAG AAGCGCGTAG GGAGCGTTAC 
AGTCAGCATC CGAAGCACAA AAGTGCACCA GTCTCTGTGA ATCGGTCGGA ATCCTACAAG 
GAGCGCTTGT CCAACAAGAG GAACCGCAAC AGTCGCCGCA AGACCTCTGA TCCAAGCTTG 
TCGTCGCGTC CCAATGATGA ACAACTGGAC TTGGGTCTAT CGAATGCCAC TTATGTGGGC 
AGTTCGAATT CTAGTCTCTC TTCAGCTGGC GGCAGCGAGA GTCCCAGCAC GTCAATGGAG 
CACTTTGCTG CACCCGGAGC AGCGGGTGGC GTCCAGGTGC CGCCAATGGG ATTGAACCAG 
AACCAGCACC CCCATCTGCT CATCCAGCAG CACGCCCAGC AGTACTGCCA GCAGGATTCC 
TTTCAGGCGG GTTTGGCAGG GGCCGCTGGG AGCAGTGCAG CTAGCAACTC TAGTTTCTGG 
AATGCTGGCC ACCCATTGCC TGTAGCTCGT TGGACGCTGG AGAGCGAGGA TGAAGACGAC 
GTGAACGAGG CGGACTGGAG TTCCATGGTG GCCGCAGAGG TGTTGGCAGC CTTAACGGAC 
GCTGAGAAGA AGCGTCAGGA GATTATAAAT GAAATCTATC AAACTGAACG CAACCATGTG 
CGCACCCTAA AGCTGCTGGA TCGATTATTC TTCCTGCCAC TCTACGAGAG TGGATTGCTG 
TCCCAGGATC ATTTGCTGTT GTTGTTCCCG CCCGCCTTGC TGTCGCTCCG TGAGATTCAT 
GGCGCCTTCG AGCAGAGTCT TAAGCAACGA CGCATCGAGC ATAACCACGT GGTGAACACC 
ATAGGGGATC TGCTCGCCGA CATGTTCGAT GGTCAGTCTG GAGTTGTTCT TTGCGAGTTT 
GCGGCTCAGT TCTGTGCCCG CCAGCAAATC GCCTTGGAGG CACTCAAGGA GAAGCGCAAC 
AAGGACGAGA TGCTGCAAAA GCTATTGAAA AAGTCGGAGT CACACAAGGC ATGTCGCCGC 
CTCGAGCTAA AGGACTTGCT GCCCACCGTG CTGCAGCGCC TCACCAAGTA TCCGCTTTTG 
TTTGAGAACC TTTACAAGGT GACCGTGCGC TTGCTGCCAG AAAACACCAC CGAGGCGGAA 
GCCATTCAAC GGGCAGTGGA ATCCTCTAAA AGGATTCTTG TCGAGGTCAA CCAGGCAGTA 
AAGACAGCAG AGGATGCTCA CAAGCTGCAA AACATTCAGC GTAAGTTAGA CAGATCCTCC 
TATGACAAGG AAGAGTTTAA GAAATTGGAC CTGACCCAAC ATCACCTCAT CCATGACGGC 
AATCTGACGA TCAAGAAGAA TCCTAGCGTG CAGCTACATG GACTTCTGTT TGAAAACATG 
ATTGTTTTGC TGACCAAGCA GGATGATAAA TATTATCTAA AGAACTTGCA CACCCCGCTA 
TCGATCACCA ATAAGCCAGT CAGTCCAATT ATGAGCATTG ATGCGGACAC TTTGATCCGG 
CAGGAGGCGG CTGATAAAAA TTCCTTTTTC CTCATCAAGA TGAAGACATC ACAAATGTTG 
GAGCTACGCG CGCCTAGTAG CTCGGAGTGC AAGACATGGT TTAAACACTT CTCGGATGTG 
GCTGCTCGTC AGTCTAAGAA TCGTTCAAAG AACGCATCAA GCAACCATGA CACGAGTATT 
AGTGATCCAG CTCTCGCCGC TATTCCGCAT TCCAACACCA AAGAGTCGTT GGAGTTGAGC 
ACTGATACAG TACAGCCATT GGCTGCGACA GCCACATTGA CTACCACACC ATTGGCCCCA 
ATGCTGCCTA TAGCCACGGT TACACCGGCT CCAGCGACCA ATAATAGTAA CGTTAGCTCT 
CTTACTGGAG TTCAGTTGCG AAACCCTCAA CGGGATGCGA CAGCAAGTGA ATCTGATGCG 
GATTATGTAA ACACACCAAA GCCGCGTTCG AGCCAAAATG AAGTTAATCG CACTATGTCC 
ATAAGAAGCA CTGGCGAACC CATTCAGAAG TATTCGGCGA ATGGGACGGA AGCAAACGAC 
GTTACTTTAC GACACTCTCA GTCGACTAGG GAATCGGTTA GACCAGGATC TACTGGGGAG 
GAGCGAAACT CCACGTATGG TATGGTTGGA GGTAACTCCA AACGCGACAG CGCCAGCATT 
GTCTGCTCGA ACAACTCGAA CAACACGCGC ACCCTTCTGA TGCAGAGTCC CTTGGTGGAC 
CCTACGGCCA TTCAGGTCAG CATTAGTCCC GCTCACACAG CGGAACCTGT GTTGACACCA 
GGAGAGAAGT TACGCCGCTT GGACGCCTCC ATTAGGAATG ATTTGCTGGA GAAGCAGAAA 
ATCATTTGTG ATATCTTCCG TTTGCCAGTG GAACACTACG ACCAGATTGT GGACATTGCC 
ATGATGCCAG AGGCGCCGAA AGACAGTGCA GATATTGCTT TAGCTGCTTA CGATCAGATT 
CAAACCCTGA CCAAGATGCT GAACGAGTAC ATGCACGTCA CGCCTGAGCA AGAGGTCTCA 
GCGGTGTCCA CGGCGGTTTG TGGCCACTGT CACGAGAAGG AAAAGCTTCG TAAGAAGGTG 
GCACCATCCT CATCGTTTTC TTCATCACCA CCACCGCTGC CGCCTCCCAA TAGGCAGCAT 
GCCCAGGCTC AGGCCCAGAT ACCGCCATCG CGGCTGATGC CCAAACTACA AACTCTTGAT 
CTTGACGAAG TTGCCATACA CGAAGACGAT GACGGATACT GTGAGATCGA CGAACTGCGC 
TTACCGGCTA TTCCGTCCAA ACCACATGAG CGGCCCACAA CGCCACTGGC TCCTTTCAAT 
ACTGAGCCGA AAACTTCACA ATCTGTTATA GATGCCTCGA AACGTCAATC CACTGATGCC 
GTTCCGGAGG GATTACTGGA ACAAGAACCA CTCGAAGGCG ATAAGACGGA GACCAAGGGT 
GAAGATAATG AAGTGAAAAC TGTGCCGTCA GATAAGCTAA GTGAATCATG CAATGAAGAG 
AGGCAATGTG TGGAGGCGGA TATCACAAAG GAAGTGGCAG ATCCAACGAC CTCTAAGAAT 
GAAGCTGCAG CATCGGTGGA TGAATTACCA AGCCAGAGCC GGGAGATAAA AACGGCTGAA 
AACGCAAGCA AATCTGTAGC TGACAAAAAG GAAGACAACG AGGAAACCAT CGAAGAAGGT 
GTGGCATCCA CGGTCGATAG CTCCACTCAA ACATCACCAA CTGAATCGCC GAAAGAGACG 
GATAAGTTAA CTGGAGGATC GAGCAGCACC TGTGGGCCGA ATCGCATTCA GCACGCTAGT 
GTGCTGGAGC CGAGTGTGCC CTGCCATGCA CTCAGCAGCA TTGTAACAAT ACTGAATGAG 
CAGATTTCCA TGCTTTTGCC AAAAATTAAC GAACGCGATA TGGAAAGGGA GCGATTGCGT 
AAAGAGAATC AACACCTTCG CGAGCTCTTG AGTGCGCTGC ATGATCGACA GCGAGTTGAT 
GAAGTAAAGG AAACTCCGTT TGATCTAAAG AAGCTGATGC ATGCTGAGGA TGTAGAGTTT 
GACGATGATA TTGACGCCAT TTCCAACAGT TCGCTGACGC CAACGCCTAC GCCGATTCCC 
ACAGCATCAC CAAGCGCCAG CGGCCAGGTA GAGACAGCGG AAGCCATGAG GATTACTAGC 
ACTGAGGATG AGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGEF2 ( NM_001259452.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246381.1 Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant G, mRNA.

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CloneID	OFa12368
Clone ID Related Accession (Same CDS sequence)	NM_001259452.2
Accession Version	NM_001259452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho guanine nucleotide exchange factor 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259452.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTC AGCATCCGAA GCACAAAAGT 
GCACCAGTCT CTGTGAATCG GTCGGAATCC TACAAGGAGC GCTTGTCCAA CAAGAGGAAC 
CGCAACAGTC GCCGCAAGAC CTCTGATCCA AGCTTGTCGT CGCGTCCCAA TGATGAACAA 
CTGGACTTGG GTCTATCGAA TGCCACTTAT GTGGGCAGTT CGAATTCTAG TCTCTCTTCA 
GCTGGCGGCA GCGAGAGTCC CAGCACGTCA ATGGAGCACT TTGCTGCACC CGGAGCAGCG 
GGTGGCGTCC AGGTGCCGCC AATGGGATTG AACCAGAACC AGCACCCCCA TCTGCTCATC 
CAGCAGCACG CCCAGCAGTA CTGCCAGCAG GATTCCTTTC AGGCGGGTTT GGCAGGGGCC 
GCTGGGAGCA GTGCAGCTAG CAACTCTAGT TTCTGGAATG CTGGCCACCC ATTGCCTGTA 
GCTCGTTGGA CGCTGGAGAG CGAGGATGAA GACGACGTGA ACGAGGCGGA CTGGAGTTCC 
ATGGTGGCCG CAGAGGTGTT GGCAGCCTTA ACGGACGCTG AGAAGAAGCG TCAGGAGATT 
ATAAATGAAA TCTATCAAAC TGAACGCAAC CATGTGCGCA CCCTAAAGCT GCTGGATCGA 
TTATTCTTCC TGCCACTCTA CGAGAGTGGA TTGCTGTCCC AGGATCATTT GCTGTTGTTG 
TTCCCGCCCG CCTTGCTGTC GCTCCGTGAG ATTCATGGCG CCTTCGAGCA GAGTCTTAAG 
CAACGACGCA TCGAGCATAA CCACGTGGTG AACACCATAG GGGATCTGCT CGCCGACATG 
TTCGATGGTC AGTCTGGAGT TGTTCTTTGC GAGTTTGCGG CTCAGTTCTG TGCCCGCCAG 
CAAATCGCCT TGGAGGCACT CAAGGAGAAG CGCAACAAGG ACGAGATGCT GCAAAAGCTA 
TTGAAAAAGT CGGAGTCACA CAAGGCATGT CGCCGCCTCG AGCTAAAGGA CTTGCTGCCC 
ACCGTGCTGC AGCGCCTCAC CAAGTATCCG CTTTTGTTTG AGAACCTTTA CAAGGTGACC 
GTGCGCTTGC TGCCAGAAAA CACCACCGAG GCGGAAGCCA TTCAACGGGC AGTGGAATCC 
TCTAAAAGGA TTCTTGTCGA GGTCAACCAG GCAGTAAAGA CAGCAGAGGA TGCTCACAAG 
CTGCAAAACA TTCAGCGTAA GTTAGACAGA TCCTCCTATG ACAAGGAAGA GTTTAAGAAA 
TTGGACCTGA CCCAACATCA CCTCATCCAT GACGGCAATC TGACGATCAA GAAGAATCCT 
AGCGTGCAGC TACATGGACT TCTGTTTGAA AACATGATTG TTTTGCTGAC CAAGCAGGAT 
GATAAATATT ATCTAAAGAA CTTGCACACC CCGCTATCGA TCACCAATAA GCCAGTCAGT 
CCAATTATGA GCATTGATGC GGACACTTTG ATCCGGCAGG AGGCGGCTGA TAAAAATTCC 
TTTTTCCTCA TCAAGATGAA GACATCACAA ATGTTGGAGC TACGCGCGCC TAGTAGCTCG 
GAGTGCAAGA CATGGTTTAA ACACTTCTCG GATGTGGCTG CTCGTCAGTC TAAGAATCGT 
TCAAAGAACG CATCAAGCAA CCATGACACG AGTATTAGTG ATCCAGCTCT CGCCGCTATT 
CCGCATTCCA ACACCAAAGA GTCGTTGGAG TTGAGCACTG ATACAGTACA GCCATTGGCT 
GCGACAGCCA CATTGACTAC CACACCATTG GCCCCAATGC TGCCTATAGC CACGGTTACA 
CCGGCTCCAG CGACCAATAA TAGTAACGTT AGCTCTCTTA CTGGAGTTCA GTTGCGAAAC 
CCTCAACGGG ATGCGACAGC AAGTGAATCT GATGCGGATT ATGTAAACAC ACCAAAGCCG 
CGTTCGAGCC AAAATGAAGT TAATCGCACT ATGTCCATAA GAAGCACTGG CGAACCCATT 
CAGAAGTATT CGGCGAATGG GACGGAAGCA AACGACGTTA CTTTACGACA CTCTCAGTCG 
ACTAGGGAAT CGGTTAGACC AGGATCTACT GGGGAGGAGC GAAACTCCAC GTATGGTATG 
GTTGGAGGTA ACTCCAAACG CGACAGCGCC AGCATTGTCT GCTCGAACAA CTCGAACAAC 
ACGCGCACCC TTCTGATGCA GAGTCCCTTG GTGGACCCTA CGGCCATTCA GGTCAGCATT 
AGTCCCGCTC ACACAGCGGA ACCTGTGTTG ACACCAGGAG AGAAGTTACG CCGCTTGGAC 
GCCTCCATTA GGAATGATTT GCTGGAGAAG CAGAAAATCA TTTGTGATAT CTTCCGTTTG 
CCAGTGGAAC ACTACGACCA GATTGTGGAC ATTGCCATGA TGCCAGAGGC GCCGAAAGAC 
AGTGCAGATA TTGCTTTAGC TGCTTACGAT CAGATTCAAA CCCTGACCAA GATGCTGAAC 
GAGTACATGC ACGTCACGCC TGAGCAAGAG GTCTCAGCGG TGTCCACGGC GGTTTGTGGC 
CACTGTCACG AGAAGGAAAA GCTTCGTAAG AAGGTGGCAC CATCCTCATC GTTTTCTTCA 
TCACCACCAC CGCTGCCGCC TCCCAATAGG CAGCATGCCC AGGCTCAGGC CCAGATACCG 
CCATCGCGGC TGATGCCCAA ACTACAAACT CTTGATCTTG ACGAAGTTGC CATACACGAA 
GACGATGACG GATACTGTGA GATCGACGAA CTGCGCTTAC CGGCTATTCC GTCCAAACCA 
CATGAGCGGC CCACAACGCC ACTGGCTCCT TTCAATACTG AGCCGAAAAC TTCACAATCT 
GTTATAGATG CCTCGAAACG TCAATCCACT GATGCCGTTC CGGAGGGATT ACTGGAACAA 
GAACCACTCG AAGGCGATAA GACGGAGACC AAGGGTGAAG ATAATGAAGT GAAAACTGTG 
CCGTCAGATA AGCTAAGTGA ATCATGCAAT GAAGAGAGGC AATGTGTGGA GGCGGATATC 
ACAAAGGAAG TGGCAGATCC AACGACCTCT AAGAATGAAG CTGCAGCATC GGTGGATGAA 
TTACCAAGCC AGAGCCGGGA GATAAAAACG GCTGAAAACG CAAGCAAATC TGTAGCTGAC 
AAAAAGGAAG ACAACGAGGA AACCATCGAA GAAGGTGTGG CATCCACGGT CGATAGCTCC 
ACTCAAACAT CACCAACTGA ATCGCCGAAA GAGACGGATA AGTTAACTGG AGGATCGAGC 
AGCACCTGTG GGCCGAATCG CATTCAGCAC GCTAGTGTGC TGGAGCCGAG TGTGCCCTGC 
CATGCACTCA GCAGCATTGT AACAATACTG AATGAGCAGA TTTCCATGCT TTTGCCAAAA 
ATTAACGAAC GCGATATGGA AAGGGAGCGA TTGCGTAAAG AGAATCAACA CCTTCGCGAG 
CTCTTGAGTG CGCTGCATGA TCGACAGCGA GTTGATGAAG TAAAGGAAAC TCCGTTTGAT 
CTAAAGAAGC TGATGCATGC TGAGGATGTA GAGTTTGACG ATGATATTGA CGCCATTTCC 
AACAGTTCGC TGACGCCAAC GCCTACGCCG ATTCCCACAG CATCACCAAG CGCCAGCGGC 
CAGGTAGAGA CAGCGGAAGC CATGAGGATT ACTAGCACTG AGGATGAGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246381.1
CDS	474..8147
Translation	MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSVPELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVEFDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEE

Target ORF information:

RefSeq Version	NM_001259452.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259452.2

ORF Insert Sequence:

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGGATGCA CCGACTGTTA AACTTCAGGC GGCCGCTGGC 
AATAAGAACA CAGCATTACT AACGCCCAAC CAAATCCAAC ACTTGTCCGC GTCCGCCACT 
CACAGCAATC AGCAATTCCA TCATCTTCAC CACCACCACA ATCTCCACAA CAACAATTAT 
CCGCCACAGC AACAGCCAGC TAGCACCTCA CCCGCATTCC TGTCCCTCCT GCCGCGTTCC 
CTTTCGTCCT TGTCGCTGGG CACGCGCAAA AACAAGACCG AAAAGGACCT AACGACTTCA 
TCTCCCTTTG GCCTAACGAC GGATTTCCTG CAGCAGCAAC GGATGAGCCA CCAAGCGGAG 
TCGATGTCTC AATCGATGCA TCAGCACACC AGCACTCCGA CCTCGCAGCA GTTCTTCCAT 
CCGCATCAGC AACAACATCG CTTTAAGGAA ACTGGACCGA CGTCGAAAGG CAAGAACAAG 
TTCCTAATTT CGAGGAGTTT GATCGAGGAG GATGTGCCGC CACCACTGCC GCAGAGGAAT 
CCGCCCAGGC AGTTAAATTT GGACCTGAAG AACGGAAACG CGTCGCCGGG TGGGTCACAT 
CTAGTGGCTC CAGTTTCCGA TCTGGATCGC GCCACAAGTC CCCAATTAAA TAGATCCCAA 
CAACAGCAAC TGCCTAGCAG CACTGACAAC AGCCCGAGCA ATGCCAAGTC CAAGCGTTCC 
AAGATCAAAA CGAAGGCCCT ATCGGATCCT AAGATGTCCA CTCAGATGTT CCTGCAAATG 
GAATCGGCGA GTGCAGCTGG AGCAGCCGGA GGTTCCATCG AGGTAGATGG GGGTCCACCA 
CCGCTACCGC CTCGCTTGCC TGGCATGATG ACGGAGGATA TGAGCCGCGG CAGCTGTCAG 
AATCTCGCTC AACCCAATTC CGTGGGCACT GCCTTCAACT ATCCTCTGGT GTCCACAACA 
ACAGCGGTGC AGAACGATAA CCTGAACATT GCCTTTCCTT TGTCCCAACG ACCCAACATT 
GTCCAGCAGC TGCAGCAATA TCAACAGCAG CAGCAGCATC AAATGAGCGG TGGCCAGGCC 
ACAGGCGCTC TGGGACAGAC TCCGAATTTG GGAAAAAACA AGCATCGACG CGTTGGTTCT 
TCACCGGACA ATATGCATCC ACGTCATCCA GATCGGATAA CGAAAACGAC TTCGGGCTCG 
TGGGAGATTG TTGAAAAGGA TGGCGAATCC TCCCCGCCCG GAACACCGCC GCCTCCATAT 
CTATCCAGCT CCCACATGAC CGTGCTGGAA GATCCGAATG AGAACAATCG TGGAGCAGCA 
GCAGCCGGAC CTGGAGTCTT TATCGAGTCG CATCAGTTTA CGCCGATGGC GGGAGCCTCT 
TCTCCGATCC CGATATCCTT ACATTCCAGC CACATGCATG CGGCCCAGTC GAACGATACG 
CAGAAGGAGA TCATCTCGAT GGAGGACGAA AACTCGGACT TGGATGAGCC CTTCATTGAC 
GAGAACGGAC CCTTTAACAA TCTAACTCGT TTGTTAGAGG CCGAGAACGT CACTTTCCTA 
GCCATCTTCC TAAACTACGT GATCTCAAAC TCGGATCCCG CGCCACTTCT GTTTTACCTG 
ATTACTGAGT TGTACAAGGA GGGCACCTCC AAGGACATGC GGAAATGGGC CTACGAAATC 
CACTCCACAT TCCTCGTGCC GCGGGCTCCA TTGTCATGGT ATCGCCAAGA TGAATCGCTG 
GCCCGCGAGG TGGATAATGT CCTGCAGTTG GAGTATGACA AAGTGGAGAT CCTAAGGACA 
GTTTTTCTGC GTAGCCGAAA GCGGGCCAAG GACCTAATCA GTGAGCAGCT GCGTGAGTTT 
CAGCAGAAGC GCACCGCCGG CCTGGGAACC ATTTACGGAC CCACGGACGA CAAGCTGGCC 
GAGGCGAAGA CGGATAAGCT AAGGGAGCAA ATCATCGACA AATATTTAAT GCCCAATCTT 
CACGCGCTTA TTGAGGATGA GAACGGTTCA CCGCCGGAGG ATGTACGCAA GGTGGCGTTG 
TGTTCTGCTC TTTCCACCGT CATCTACCGT ATTTTCAACA CTCGTCCGCC TCCAAGCAGC 
ATCGTTGAGC GGGTCCATCA CTTTGTGAGC AGGGACAAGA GTTTCAAGTC GCGTATTATG 
GGCAAAAATC GCAAGATGAA TGTTCGTGGT CATCCATTGG TATTGCGTCA ATACTATGAA 
GTGACGCACT GCAATCATTG TCAGACGATT ATCTGGGGCG TGAGCCCGCA AGGTTATCAT 
TGTACAGACT GTAAATTGAA CATACACCGT CAGTGCTCGA AAGTAGTGGA CGAGAGTTGT 
CCCGGTCCCT TGCCCCAGGC AAAACGTCTC GCCCACAACG ACAAGATCAG TAAATTCATG 
GGCAAAATTC GACCGCGTAC CAGCGACGTC ATTGGAAATG AAAAGCGAAG TCGTCAAGAT 
GAGGAATTGG ATGTTGAGTT GACTCCAGAC CGTGGTCAGG CGTCGATTGT GCGTCAACCC 
TCTGATCGGC GACCGGATGC GAACATATCG ATAAGGTCGA ATGGAAATAC CTCCTGCAAC 
ACTTCGGGGC TGAACACCAC CGACCTGCAA AGTTCTTTTC ACGGCAGCTG TGCCAACGAC 
AGTATTAACC CCGGCGGTGG AGCCGGATGC AACATGGATT TATCCACGAG CGTGGCGTCA 
ACGACTCCGT CGACCAGTGG ATCCGTGGCA GCCGGTCTGA GTGCTTTTGC CGAACTGAAC 
GCCCTGGATA CAGTGGATAA AGAAGCGCGT AGGGAGCGTC AGCATCCGAA GCACAAAAGT 
GCACCAGTCT CTGTGAATCG GTCGGAATCC TACAAGGAGC GCTTGTCCAA CAAGAGGAAC 
CGCAACAGTC GCCGCAAGAC CTCTGATCCA AGCTTGTCGT CGCGTCCCAA TGATGAACAA 
CTGGACTTGG GTCTATCGAA TGCCACTTAT GTGGGCAGTT CGAATTCTAG TCTCTCTTCA 
GCTGGCGGCA GCGAGAGTCC CAGCACGTCA ATGGAGCACT TTGCTGCACC CGGAGCAGCG 
GGTGGCGTCC AGGTGCCGCC AATGGGATTG AACCAGAACC AGCACCCCCA TCTGCTCATC 
CAGCAGCACG CCCAGCAGTA CTGCCAGCAG GATTCCTTTC AGGCGGGTTT GGCAGGGGCC 
GCTGGGAGCA GTGCAGCTAG CAACTCTAGT TTCTGGAATG CTGGCCACCC ATTGCCTGTA 
GCTCGTTGGA CGCTGGAGAG CGAGGATGAA GACGACGTGA ACGAGGCGGA CTGGAGTTCC 
ATGGTGGCCG CAGAGGTGTT GGCAGCCTTA ACGGACGCTG AGAAGAAGCG TCAGGAGATT 
ATAAATGAAA TCTATCAAAC TGAACGCAAC CATGTGCGCA CCCTAAAGCT GCTGGATCGA 
TTATTCTTCC TGCCACTCTA CGAGAGTGGA TTGCTGTCCC AGGATCATTT GCTGTTGTTG 
TTCCCGCCCG CCTTGCTGTC GCTCCGTGAG ATTCATGGCG CCTTCGAGCA GAGTCTTAAG 
CAACGACGCA TCGAGCATAA CCACGTGGTG AACACCATAG GGGATCTGCT CGCCGACATG 
TTCGATGGTC AGTCTGGAGT TGTTCTTTGC GAGTTTGCGG CTCAGTTCTG TGCCCGCCAG 
CAAATCGCCT TGGAGGCACT CAAGGAGAAG CGCAACAAGG ACGAGATGCT GCAAAAGCTA 
TTGAAAAAGT CGGAGTCACA CAAGGCATGT CGCCGCCTCG AGCTAAAGGA CTTGCTGCCC 
ACCGTGCTGC AGCGCCTCAC CAAGTATCCG CTTTTGTTTG AGAACCTTTA CAAGGTGACC 
GTGCGCTTGC TGCCAGAAAA CACCACCGAG GCGGAAGCCA TTCAACGGGC AGTGGAATCC 
TCTAAAAGGA TTCTTGTCGA GGTCAACCAG GCAGTAAAGA CAGCAGAGGA TGCTCACAAG 
CTGCAAAACA TTCAGCGTAA GTTAGACAGA TCCTCCTATG ACAAGGAAGA GTTTAAGAAA 
TTGGACCTGA CCCAACATCA CCTCATCCAT GACGGCAATC TGACGATCAA GAAGAATCCT 
AGCGTGCAGC TACATGGACT TCTGTTTGAA AACATGATTG TTTTGCTGAC CAAGCAGGAT 
GATAAATATT ATCTAAAGAA CTTGCACACC CCGCTATCGA TCACCAATAA GCCAGTCAGT 
CCAATTATGA GCATTGATGC GGACACTTTG ATCCGGCAGG AGGCGGCTGA TAAAAATTCC 
TTTTTCCTCA TCAAGATGAA GACATCACAA ATGTTGGAGC TACGCGCGCC TAGTAGCTCG 
GAGTGCAAGA CATGGTTTAA ACACTTCTCG GATGTGGCTG CTCGTCAGTC TAAGAATCGT 
TCAAAGAACG CATCAAGCAA CCATGACACG AGTATTAGTG ATCCAGCTCT CGCCGCTATT 
CCGCATTCCA ACACCAAAGA GTCGTTGGAG TTGAGCACTG ATACAGTACA GCCATTGGCT 
GCGACAGCCA CATTGACTAC CACACCATTG GCCCCAATGC TGCCTATAGC CACGGTTACA 
CCGGCTCCAG CGACCAATAA TAGTAACGTT AGCTCTCTTA CTGGAGTTCA GTTGCGAAAC 
CCTCAACGGG ATGCGACAGC AAGTGAATCT GATGCGGATT ATGTAAACAC ACCAAAGCCG 
CGTTCGAGCC AAAATGAAGT TAATCGCACT ATGTCCATAA GAAGCACTGG CGAACCCATT 
CAGAAGTATT CGGCGAATGG GACGGAAGCA AACGACGTTA CTTTACGACA CTCTCAGTCG 
ACTAGGGAAT CGGTTAGACC AGGATCTACT GGGGAGGAGC GAAACTCCAC GTATGGTATG 
GTTGGAGGTA ACTCCAAACG CGACAGCGCC AGCATTGTCT GCTCGAACAA CTCGAACAAC 
ACGCGCACCC TTCTGATGCA GAGTCCCTTG GTGGACCCTA CGGCCATTCA GGTCAGCATT 
AGTCCCGCTC ACACAGCGGA ACCTGTGTTG ACACCAGGAG AGAAGTTACG CCGCTTGGAC 
GCCTCCATTA GGAATGATTT GCTGGAGAAG CAGAAAATCA TTTGTGATAT CTTCCGTTTG 
CCAGTGGAAC ACTACGACCA GATTGTGGAC ATTGCCATGA TGCCAGAGGC GCCGAAAGAC 
AGTGCAGATA TTGCTTTAGC TGCTTACGAT CAGATTCAAA CCCTGACCAA GATGCTGAAC 
GAGTACATGC ACGTCACGCC TGAGCAAGAG GTCTCAGCGG TGTCCACGGC GGTTTGTGGC 
CACTGTCACG AGAAGGAAAA GCTTCGTAAG AAGGTGGCAC CATCCTCATC GTTTTCTTCA 
TCACCACCAC CGCTGCCGCC TCCCAATAGG CAGCATGCCC AGGCTCAGGC CCAGATACCG 
CCATCGCGGC TGATGCCCAA ACTACAAACT CTTGATCTTG ACGAAGTTGC CATACACGAA 
GACGATGACG GATACTGTGA GATCGACGAA CTGCGCTTAC CGGCTATTCC GTCCAAACCA 
CATGAGCGGC CCACAACGCC ACTGGCTCCT TTCAATACTG AGCCGAAAAC TTCACAATCT 
GTTATAGATG CCTCGAAACG TCAATCCACT GATGCCGTTC CGGAGGGATT ACTGGAACAA 
GAACCACTCG AAGGCGATAA GACGGAGACC AAGGGTGAAG ATAATGAAGT GAAAACTGTG 
CCGTCAGATA AGCTAAGTGA ATCATGCAAT GAAGAGAGGC AATGTGTGGA GGCGGATATC 
ACAAAGGAAG TGGCAGATCC AACGACCTCT AAGAATGAAG CTGCAGCATC GGTGGATGAA 
TTACCAAGCC AGAGCCGGGA GATAAAAACG GCTGAAAACG CAAGCAAATC TGTAGCTGAC 
AAAAAGGAAG ACAACGAGGA AACCATCGAA GAAGGTGTGG CATCCACGGT CGATAGCTCC 
ACTCAAACAT CACCAACTGA ATCGCCGAAA GAGACGGATA AGTTAACTGG AGGATCGAGC 
AGCACCTGTG GGCCGAATCG CATTCAGCAC GCTAGTGTGC TGGAGCCGAG TGTGCCCTGC 
CATGCACTCA GCAGCATTGT AACAATACTG AATGAGCAGA TTTCCATGCT TTTGCCAAAA 
ATTAACGAAC GCGATATGGA AAGGGAGCGA TTGCGTAAAG AGAATCAACA CCTTCGCGAG 
CTCTTGAGTG CGCTGCATGA TCGACAGCGA GTTGATGAAG TAAAGGAAAC TCCGTTTGAT 
CTAAAGAAGC TGATGCATGC TGAGGATGTA GAGTTTGACG ATGATATTGA CGCCATTTCC 
AACAGTTCGC TGACGCCAAC GCCTACGCCG ATTCCCACAG CATCACCAAG CGCCAGCGGC 
CAGGTAGAGA CAGCGGAAGC CATGAGGATT ACTAGCACTG AGGATGAGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGEF2 ( NM_001299586.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286515.1 Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant H, mRNA.

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CloneID	OFa21838
Clone ID Related Accession (Same CDS sequence)	NM_001299586.1
Accession Version	NM_001299586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho guanine nucleotide exchange factor 2, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGCAGCAA CGGATGAGCC ACCAAGCGGA GTCGATGTCT 
CAATCGATGC ATCAGCACAC CAGCACTCCG ACCTCGCAGC AGTTCTTCCA TCCGCATCAG 
CAACAACATC GCTTTAAGGA AACTGGACCG ACGTCGAAAG GCAAGAACAA GTTCCTAATT 
TCGAGGAGTT TGATCGAGGA GGATGTGCCG CCACCACTGC CGCAGAGGAA TCCGCCCAGG 
CAGTTAAATT TGGACCTGAA GAACGGAAAC GCGTCGCCGG GTGGGTCACA TCTAGTGGCT 
CCAGTTTCCG ATCTGGATCG CGCCACAAGT CCCCAATTAA ATAGATCCCA ACAACAGCAA 
CTGCCTAGCA GCACTGACAA CAGCCCGAGC AATGCCAAGT CCAAGCGTTC CAAGATCAAA 
ACGAAGGCCC TATCGGATCC TAAGATGTCC ACTCAGATGT TCCTGCAAAT GGAATCGGCG 
AGTGCAGCTG GAGCAGCCGG AGGTTCCATC GAGGTAGATG GGGGTCCACC ACCGCTACCG 
CCTCGCTTGC CTGGCATGAT GACGGAGGAT ATGAGCCGCG GCAGCTGTCA GAATCTCGCT 
CAACCCAATT CCGTGGGCAC TGCCTTCAAC TATCCTCTGG TGTCCACAAC AACAGCGGTG 
CAGAACGATA ACCTGAACAT TGCCTTTCCT TTGTCCCAAC GACCCAACAT TGTCCAGCAG 
CTGCAGCAAT ATCAACAGCA GCAGCAGCAT CAAATGAGCG GTGGCCAGGC CACAGGCGCT 
CTGGGACAGA CTCCGAATTT GGGAAAAAAC AAGCATCGAC GCGTTGGTTC TTCACCGGAC 
AATATGCATC CACGTCATCC AGATCGGATA ACGAAAACGA CTTCGGGCTC GTGGGAGATT 
GTTGAAAAGG ATGGCGAATC CTCCCCGCCC GGAACACCGC CGCCTCCATA TCTATCCAGC 
TCCCACATGA CCGTGCTGGA AGATCCGAAT GAGAACAATC GTGGAGCAGC AGCAGCCGGA 
CCTGGAGTCT TTATCGAGTC GCATCAGTTT ACGCCGATGG CGGGAGCCTC TTCTCCGATC 
CCGATATCCT TACATTCCAG CCACATGCAT GCGGCCCAGT CGAACGATAC GCAGAAGGAG 
ATCATCTCGA TGGAGGACGA AAACTCGGAC TTGGATGAGC CCTTCATTGA CGAGAACGGA 
CCCTTTAACA ATCTAACTCG TTTGTTAGAG GCCGAGAACG TCACTTTCCT AGCCATCTTC 
CTAAACTACG TGATCTCAAA CTCGGATCCC GCGCCACTTC TGTTTTACCT GATTACTGAG 
TTGTACAAGG AGGGCACCTC CAAGGACATG CGGAAATGGG CCTACGAAAT CCACTCCACA 
TTCCTCGTGC CGCGGGCTCC ATTGTCATGG TATCGCCAAG ATGAATCGCT GGCCCGCGAG 
GTGGATAATG TCCTGCAGTT GGAGTATGAC AAAGTGGAGA TCCTAAGGAC AGTTTTTCTG 
CGTAGCCGAA AGCGGGCCAA GGACCTAATC AGTGAGCAGC TGCGTGAGTT TCAGCAGAAG 
CGCACCGCCG GCCTGGGAAC CATTTACGGA CCCACGGACG ACAAGCTGGC CGAGGCGAAG 
ACGGATAAGC TAAGGGAGCA AATCATCGAC AAATATTTAA TGCCCAATCT TCACGCGCTT 
ATTGAGGATG AGAACGGTTC ACCGCCGGAG GATGTACGCA AGGTGGCGTT GTGTTCTGCT 
CTTTCCACCG TCATCTACCG TATTTTCAAC ACTCGTCCGC CTCCAAGCAG CATCGTTGAG 
CGGGTCCATC ACTTTGTGAG CAGGGACAAG AGTTTCAAGT CGCGTATTAT GGGCAAAAAT 
CGCAAGATGA ATGTTCGTGG TCATCCATTG GTATTGCGTC AATACTATGA AGTGACGCAC 
TGCAATCATT GTCAGACGAT TATCTGGGGC GTGAGCCCGC AAGGTTATCA TTGTACAGAC 
TGTAAATTGA ACATACACCG TCAGTGCTCG AAAGTAGTGG ACGAGAGTTG TCCCGGTCCC 
TTGCCCCAGG CAAAACGTCT CGCCCACAAC GACAAGATCA GTAAATTCAT GGGCAAAATT 
CGACCGCGTA CCAGCGACGT CATTGGAAAT GAAAAGCGAA GTCGTCAAGA TGAGGAATTG 
GATGTTGAGT TGACTCCAGA CCGTGGTCAG GCGTCGATTG TGCGTCAACC CTCTGATCGG 
CGACCGGATG CGAACATATC GATAAGGTCG AATGGAAATA CCTCCTGCAA CACTTCGGGG 
CTGAACACCA CCGACCTGCA AAGTTCTTTT CACGGCAGCT GTGCCAACGA CAGTATTAAC 
CCCGGCGGTG GAGCCGGATG CAACATGGAT TTATCCACGA GCGTGGCGTC AACGACTCCG 
TCGACCAGTG GATCCGTGGC AGCCGGTCTG AGTGCTTTTG CCGAACTGAA CGCCCTGGAT 
ACAGTGGATA AAGAAGCGCG TAGGGAGCGT TACAGTCAGC ATCCGAAGCA CAAAAGTGCA 
CCAGTCTCTG TGAATCGGTC GGAATCCTAC AAGGAGCGCT TGTCCAACAA GAGGAACCGC 
AACAGTCGCC GCAAGACCTC TGATCCAAGC TTGTCGTCGC GTCCCAATGA TGAACAACTG 
GACTTGGGTC TATCGAATGC CACTTATGTG GGCAGTTCGA ATTCTAGTCT CTCTTCAGCT 
GGCGGCAGCG AGAGTCCCAG CACGTCAATG GAGCACTTTG CTGCACCCGG AGCAGCGGGT 
GGCGTCCAGG TGCCGCCAAT GGGATTGAAC CAGAACCAGC ACCCCCATCT GCTCATCCAG 
CAGCACGCCC AGCAGTACTG CCAGCAGGAT TCCTTTCAGG CGGGTTTGGC AGGGGCCGCT 
GGGAGCAGTG CAGCTAGCAA CTCTAGTTTC TGGAATGCTG GCCACCCATT GCCTGTAGCT 
CGTTGGACGC TGGAGAGCGA GGATGAAGAC GACGTGAACG AGGCGGACTG GAGTTCCATG 
GTGGCCGCAG AGGTGTTGGC AGCCTTAACG GACGCTGAGA AGAAGCGTCA GGAGATTATA 
AATGAAATCT ATCAAACTGA ACGCAACCAT GTGCGCACCC TAAAGCTGCT GGATCGATTA 
TTCTTCCTGC CACTCTACGA GAGTGGATTG CTGTCCCAGG ATCATTTGCT GTTGTTGTTC 
CCGCCCGCCT TGCTGTCGCT CCGTGAGATT CATGGCGCCT TCGAGCAGAG TCTTAAGCAA 
CGACGCATCG AGCATAACCA CGTGGTGAAC ACCATAGGGG ATCTGCTCGC CGACATGTTC 
GATGGTCAGT CTGGAGTTGT TCTTTGCGAG TTTGCGGCTC AGTTCTGTGC CCGCCAGCAA 
ATCGCCTTGG AGGCACTCAA GGAGAAGCGC AACAAGGACG AGATGCTGCA AAAGCTATTG 
AAAAAGTCGG AGTCACACAA GGCATGTCGC CGCCTCGAGC TAAAGGACTT GCTGCCCACC 
GTGCTGCAGC GCCTCACCAA GTATCCGCTT TTGTTTGAGA ACCTTTACAA GGTGACCGTG 
CGCTTGCTGC CAGAAAACAC CACCGAGGCG GAAGCCATTC AACGGGCAGT GGAATCCTCT 
AAAAGGATTC TTGTCGAGGT CAACCAGGCA GTAAAGACAG CAGAGGATGC TCACAAGCTG 
CAAAACATTC AGCGTAAGTT AGACAGATCC TCCTATGACA AGGAAGAGTT TAAGAAATTG 
GACCTGACCC AACATCACCT CATCCATGAC GGCAATCTGA CGATCAAGAA GAATCCTAGC 
GTGCAGCTAC ATGGACTTCT GTTTGAAAAC ATGATTGTTT TGCTGACCAA GCAGGATGAT 
AAATATTATC TAAAGAACTT GCACACCCCG CTATCGATCA CCAATAAGCC AGTCAGTCCA 
ATTATGAGCA TTGATGCGGA CACTTTGATC CGGCAGGAGG CGGCTGATAA AAATTCCTTT 
TTCCTCATCA AGATGAAGAC ATCACAAATG TTGGAGCTAC GCGCGCCTAG TAGCTCGGAG 
TGCAAGACAT GGTTTAAACA CTTCTCGGAT GTGGCTGCTC GTCAGTCTAA GAATCGTTCA 
AAGAACGCAT CAAGCAACCA TGACACGAGT ATTAGTGATC CAGCTCTCGC CGCTATTCCG 
CATTCCAACA CCAAAGAGTC GTTGGAGTTG AGCACTGATA CAGTACAGCC ATTGGCTGCG 
ACAGCCACAT TGACTACCAC ACCATTGGCC CCAATGCTGC CTATAGCCAC GGTTACACCG 
GCTCCAGCGA CCAATAATAG TAACGTTAGC TCTCTTACTG GAGTTCAGTT GCGAAACCCT 
CAACGGGATG CGACAGCAAG TGAATCTGAT GCGGATTATG TAAACACACC AAAGCCGCGT 
TCGAGCCAAA ATGAAGTTAA TCGCACTATG TCCATAAGAA GCACTGGCGA ACCCATTCAG 
AAGTATTCGG CGAATGGGAC GGAAGCAAAC GACGTTACTT TACGACACTC TCAGTCGACT 
AGGGAATCGG TTAGACCAGG ATCTACTGGG GAGGAGCGAA ACTCCACGTA TGGTATGGTT 
GGAGGTAACT CCAAACGCGA CAGCGCCAGC ATTGTCTGCT CGAACAACTC GAACAACACG 
CGCACCCTTC TGATGCAGAG TCCCTTGGTG GACCCTACGG CCATTCAGGT CAGCATTAGT 
CCCGCTCACA CAGCGGAACC TGTGTTGACA CCAGGAGAGA AGTTACGCCG CTTGGACGCC 
TCCATTAGGA ATGATTTGCT GGAGAAGCAG AAAATCATTT GTGATATCTT CCGTTTGCCA 
GTGGAACACT ACGACCAGAT TGTGGACATT GCCATGATGC CAGAGGCGCC GAAAGACAGT 
GCAGATATTG CTTTAGCTGC TTACGATCAG ATTCAAACCC TGACCAAGAT GCTGAACGAG 
TACATGCACG TCACGCCTGA GCAAGAGGTC TCAGCGGTGT CCACGGCGGT TTGTGGCCAC 
TGTCACGAGA AGGAAAAGCT TCGTAAGAAG GTGGCACCAT CCTCATCGTT TTCTTCATCA 
CCACCACCGC TGCCGCCTCC CAATAGGCAG CATGCCCAGG CTCAGGCCCA GATACCGCCA 
TCGCGGCTGA TGCCCAAACT ACAAACTCTT GATCTTGACG AAGTTGCCAT ACACGAAGAC 
GATGACGGAT ACTGTGAGAT CGACGAACTG CGCTTACCGG CTATTCCGTC CAAACCACAT 
GAGCGGCCCA CAACGCCACT GGCTCCTTTC AATACTGAGC CGAAAACTTC ACAATCTGTT 
ATAGATGCCT CGAAACGTCA ATCCACTGAT GCCGTTCCGG AGGGATTACT GGAACAAGAA 
CCACTCGAAG GCGATAAGAC GGAGACCAAG GGTGAAGATA ATGAAGTGAA AACTGTGCCG 
TCAGATAAGC TAAGTGAATC ATGCAATGAA GAGAGGCAAT GTGTGGAGGC GGATATCACA 
AAGGAAGTGG CAGATCCAAC GACCTCTAAG AATGAAGCTG CAGCATCGGT GGATGAATTA 
CCAAGCCAGA GCCGGGAGAT AAAAACGGCT GAAAACGCAA GCAAATCTGT AGCTGACAAA 
AAGGAAGACA ACGAGGAAAC CATCGAAGAA GGTGTGGCAT CCACGGTCGA TAGCTCCACT 
CAAACATCAC CAACTGAATC GCCGAAAGAG ACGGATAAGT TAACTGGAGG ATCGAGCAGC 
ACCTGTGGGC CGAATCGCAT TCAGCACGCT AGTGTGCTGG AGCCGAGTGT GCCCTGCCAT 
GCACTCAGCA GCATTGTAAC AATACTGAAT GAGCAGATTT CCATGCTTTT GCCAAAAATT 
AACGAACGCG ATATGGAAAG GGAGCGATTG CGTAAAGAGA ATCAACACCT TCGCGAGCTC 
TTGAGTGCGC TGCATGATCG ACAGCGAGTT GATGAAGTAA AGGAAACTCC GTTTGATCTA 
AAGAAGCTGA TGCATGCTGA GGATGTAGAG TTTGACGATG ATATTGACGC CATTTCCAAC 
AGTTCGCTGA CGCCAACGCC TACGCCGATT CCCACAGCAT CACCAAGCGC CAGCGGCCAG 
GTAGAGACAG CGGAAGCCAT GAGGATTACT AGCACTGAGG ATGAGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286515.1
CDS	463..7833
Translation	MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSVPELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVEFDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEE

Target ORF information:

RefSeq Version	NM_001299586.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho guanine nucleotide exchange factor 2 (RhoGEF2), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299586.1

ORF Insert Sequence:

ATGGATGACC CATCAATCAA AAAACGGTTA CTAGATTTAT ATACTGACGA ACATGAATAC 
GATGAGGTTC AAGAGATACC AGAAGAGTCA AGCATTCAGC CCCCGGAGAC ATCAACGAGC 
CATACCAGCA CAAACGGATC CAGCCACTCA GGCCCTGGAA CTGCAACTGG ACCAGGAGCG 
ACATCTGCAG GCCCGTCAGC GGGTGCGCCA CAGTCACCAG TAATTGTTGT GGACTCGGTT 
CCCGAGCTGC CAGCGCCCAA GCAGAAATCT GTTAAGAACT CGAAGAGCAA ACAGAAGCAA 
AAGCAGTTGG CGAACAAATC GAAGATTCCA CGATCTCCTT CGTTGGCGAG CAGCCTGAGT 
AGTCTGGCCA GCAGCCTTAG TGGTCATAGG GATCGGGACA AAGATCGGGA CAAGGATCGG 
GAGAACCAGA ACGCCGTGCC GCCGCAGACG CCGCCGTTGC CACCGAGCTA CAAGCAGAAC 
CAAATGAATG GTGACTCTAC GGCTGCAGCT GGTGGTGGTG TTTCTGCCCC AGCCACGCCC 
ACCACCGCCA ATAATAACAA TGCGAGCCAC AACAACGGCA GCATAATGGG CGGAGGCGTG 
CAATTGAATC AATCGGACAA CTCCAACCCC GTTCTCCAGG CGCCGGGGGA GCGCAGTAGC 
CTCAATCTGA CTCCCCTCTC CCGGGATCTG AGTGGTGGCC ACACCCAGGA GTCCACGACG 
CCAGCGACCA CGCCGAGTAC GCCAAGCCTA GCATTACCAA AAAATTTTCA GTATTTAACC 
CTGACTGTGC GAAAAGATAG CAACGGATAC GGGATGAAGG TTTCCGGAGA TAATCCTGTG 
TTTGTGGAGA GCGTTAAACC CGGAGGCGCA GCGGAGATTG CAGGCCTGGT TGCTGGCGAT 
ATGATACTAA GGGTAAACGG CCATGAAGTG CGACTAGAGA AGCATCCAAC TGTAGTGGGT 
CTTATAAAAG CCTCGACAAC TGTTGAGCTG GCGGTGAAGC GAAGTCAGAA GCTAACGCGA 
CCCTCTTCGG TATCAGTGGT AACGCCCTCG ACACCCATTC TCTCTGGACG AGATCGTACC 
GCTTCAATAA CTGGGCCACA GCCGGTGGAC AGTATTAAAC GAAGGGAGAT GGAGACTTAC 
AAAATTCAGA CGTTGCAGAA AATGCTGGAG CAGGAGAAAC TAAATCTGGA GCGATTAAAA 
AGCGATCAAA ATAATCCGAG CTACAAGCTA TCTGAGGCGA ATATCCGTAA GCTGCGCGAG 
CAACTTCATC AAGTGGGAGC TGAGCAGCAA CGGATGAGCC ACCAAGCGGA GTCGATGTCT 
CAATCGATGC ATCAGCACAC CAGCACTCCG ACCTCGCAGC AGTTCTTCCA TCCGCATCAG 
CAACAACATC GCTTTAAGGA AACTGGACCG ACGTCGAAAG GCAAGAACAA GTTCCTAATT 
TCGAGGAGTT TGATCGAGGA GGATGTGCCG CCACCACTGC CGCAGAGGAA TCCGCCCAGG 
CAGTTAAATT TGGACCTGAA GAACGGAAAC GCGTCGCCGG GTGGGTCACA TCTAGTGGCT 
CCAGTTTCCG ATCTGGATCG CGCCACAAGT CCCCAATTAA ATAGATCCCA ACAACAGCAA 
CTGCCTAGCA GCACTGACAA CAGCCCGAGC AATGCCAAGT CCAAGCGTTC CAAGATCAAA 
ACGAAGGCCC TATCGGATCC TAAGATGTCC ACTCAGATGT TCCTGCAAAT GGAATCGGCG 
AGTGCAGCTG GAGCAGCCGG AGGTTCCATC GAGGTAGATG GGGGTCCACC ACCGCTACCG 
CCTCGCTTGC CTGGCATGAT GACGGAGGAT ATGAGCCGCG GCAGCTGTCA GAATCTCGCT 
CAACCCAATT CCGTGGGCAC TGCCTTCAAC TATCCTCTGG TGTCCACAAC AACAGCGGTG 
CAGAACGATA ACCTGAACAT TGCCTTTCCT TTGTCCCAAC GACCCAACAT TGTCCAGCAG 
CTGCAGCAAT ATCAACAGCA GCAGCAGCAT CAAATGAGCG GTGGCCAGGC CACAGGCGCT 
CTGGGACAGA CTCCGAATTT GGGAAAAAAC AAGCATCGAC GCGTTGGTTC TTCACCGGAC 
AATATGCATC CACGTCATCC AGATCGGATA ACGAAAACGA CTTCGGGCTC GTGGGAGATT 
GTTGAAAAGG ATGGCGAATC CTCCCCGCCC GGAACACCGC CGCCTCCATA TCTATCCAGC 
TCCCACATGA CCGTGCTGGA AGATCCGAAT GAGAACAATC GTGGAGCAGC AGCAGCCGGA 
CCTGGAGTCT TTATCGAGTC GCATCAGTTT ACGCCGATGG CGGGAGCCTC TTCTCCGATC 
CCGATATCCT TACATTCCAG CCACATGCAT GCGGCCCAGT CGAACGATAC GCAGAAGGAG 
ATCATCTCGA TGGAGGACGA AAACTCGGAC TTGGATGAGC CCTTCATTGA CGAGAACGGA 
CCCTTTAACA ATCTAACTCG TTTGTTAGAG GCCGAGAACG TCACTTTCCT AGCCATCTTC 
CTAAACTACG TGATCTCAAA CTCGGATCCC GCGCCACTTC TGTTTTACCT GATTACTGAG 
TTGTACAAGG AGGGCACCTC CAAGGACATG CGGAAATGGG CCTACGAAAT CCACTCCACA 
TTCCTCGTGC CGCGGGCTCC ATTGTCATGG TATCGCCAAG ATGAATCGCT GGCCCGCGAG 
GTGGATAATG TCCTGCAGTT GGAGTATGAC AAAGTGGAGA TCCTAAGGAC AGTTTTTCTG 
CGTAGCCGAA AGCGGGCCAA GGACCTAATC AGTGAGCAGC TGCGTGAGTT TCAGCAGAAG 
CGCACCGCCG GCCTGGGAAC CATTTACGGA CCCACGGACG ACAAGCTGGC CGAGGCGAAG 
ACGGATAAGC TAAGGGAGCA AATCATCGAC AAATATTTAA TGCCCAATCT TCACGCGCTT 
ATTGAGGATG AGAACGGTTC ACCGCCGGAG GATGTACGCA AGGTGGCGTT GTGTTCTGCT 
CTTTCCACCG TCATCTACCG TATTTTCAAC ACTCGTCCGC CTCCAAGCAG CATCGTTGAG 
CGGGTCCATC ACTTTGTGAG CAGGGACAAG AGTTTCAAGT CGCGTATTAT GGGCAAAAAT 
CGCAAGATGA ATGTTCGTGG TCATCCATTG GTATTGCGTC AATACTATGA AGTGACGCAC 
TGCAATCATT GTCAGACGAT TATCTGGGGC GTGAGCCCGC AAGGTTATCA TTGTACAGAC 
TGTAAATTGA ACATACACCG TCAGTGCTCG AAAGTAGTGG ACGAGAGTTG TCCCGGTCCC 
TTGCCCCAGG CAAAACGTCT CGCCCACAAC GACAAGATCA GTAAATTCAT GGGCAAAATT 
CGACCGCGTA CCAGCGACGT CATTGGAAAT GAAAAGCGAA GTCGTCAAGA TGAGGAATTG 
GATGTTGAGT TGACTCCAGA CCGTGGTCAG GCGTCGATTG TGCGTCAACC CTCTGATCGG 
CGACCGGATG CGAACATATC GATAAGGTCG AATGGAAATA CCTCCTGCAA CACTTCGGGG 
CTGAACACCA CCGACCTGCA AAGTTCTTTT CACGGCAGCT GTGCCAACGA CAGTATTAAC 
CCCGGCGGTG GAGCCGGATG CAACATGGAT TTATCCACGA GCGTGGCGTC AACGACTCCG 
TCGACCAGTG GATCCGTGGC AGCCGGTCTG AGTGCTTTTG CCGAACTGAA CGCCCTGGAT 
ACAGTGGATA AAGAAGCGCG TAGGGAGCGT TACAGTCAGC ATCCGAAGCA CAAAAGTGCA 
CCAGTCTCTG TGAATCGGTC GGAATCCTAC AAGGAGCGCT TGTCCAACAA GAGGAACCGC 
AACAGTCGCC GCAAGACCTC TGATCCAAGC TTGTCGTCGC GTCCCAATGA TGAACAACTG 
GACTTGGGTC TATCGAATGC CACTTATGTG GGCAGTTCGA ATTCTAGTCT CTCTTCAGCT 
GGCGGCAGCG AGAGTCCCAG CACGTCAATG GAGCACTTTG CTGCACCCGG AGCAGCGGGT 
GGCGTCCAGG TGCCGCCAAT GGGATTGAAC CAGAACCAGC ACCCCCATCT GCTCATCCAG 
CAGCACGCCC AGCAGTACTG CCAGCAGGAT TCCTTTCAGG CGGGTTTGGC AGGGGCCGCT 
GGGAGCAGTG CAGCTAGCAA CTCTAGTTTC TGGAATGCTG GCCACCCATT GCCTGTAGCT 
CGTTGGACGC TGGAGAGCGA GGATGAAGAC GACGTGAACG AGGCGGACTG GAGTTCCATG 
GTGGCCGCAG AGGTGTTGGC AGCCTTAACG GACGCTGAGA AGAAGCGTCA GGAGATTATA 
AATGAAATCT ATCAAACTGA ACGCAACCAT GTGCGCACCC TAAAGCTGCT GGATCGATTA 
TTCTTCCTGC CACTCTACGA GAGTGGATTG CTGTCCCAGG ATCATTTGCT GTTGTTGTTC 
CCGCCCGCCT TGCTGTCGCT CCGTGAGATT CATGGCGCCT TCGAGCAGAG TCTTAAGCAA 
CGACGCATCG AGCATAACCA CGTGGTGAAC ACCATAGGGG ATCTGCTCGC CGACATGTTC 
GATGGTCAGT CTGGAGTTGT TCTTTGCGAG TTTGCGGCTC AGTTCTGTGC CCGCCAGCAA 
ATCGCCTTGG AGGCACTCAA GGAGAAGCGC AACAAGGACG AGATGCTGCA AAAGCTATTG 
AAAAAGTCGG AGTCACACAA GGCATGTCGC CGCCTCGAGC TAAAGGACTT GCTGCCCACC 
GTGCTGCAGC GCCTCACCAA GTATCCGCTT TTGTTTGAGA ACCTTTACAA GGTGACCGTG 
CGCTTGCTGC CAGAAAACAC CACCGAGGCG GAAGCCATTC AACGGGCAGT GGAATCCTCT 
AAAAGGATTC TTGTCGAGGT CAACCAGGCA GTAAAGACAG CAGAGGATGC TCACAAGCTG 
CAAAACATTC AGCGTAAGTT AGACAGATCC TCCTATGACA AGGAAGAGTT TAAGAAATTG 
GACCTGACCC AACATCACCT CATCCATGAC GGCAATCTGA CGATCAAGAA GAATCCTAGC 
GTGCAGCTAC ATGGACTTCT GTTTGAAAAC ATGATTGTTT TGCTGACCAA GCAGGATGAT 
AAATATTATC TAAAGAACTT GCACACCCCG CTATCGATCA CCAATAAGCC AGTCAGTCCA 
ATTATGAGCA TTGATGCGGA CACTTTGATC CGGCAGGAGG CGGCTGATAA AAATTCCTTT 
TTCCTCATCA AGATGAAGAC ATCACAAATG TTGGAGCTAC GCGCGCCTAG TAGCTCGGAG 
TGCAAGACAT GGTTTAAACA CTTCTCGGAT GTGGCTGCTC GTCAGTCTAA GAATCGTTCA 
AAGAACGCAT CAAGCAACCA TGACACGAGT ATTAGTGATC CAGCTCTCGC CGCTATTCCG 
CATTCCAACA CCAAAGAGTC GTTGGAGTTG AGCACTGATA CAGTACAGCC ATTGGCTGCG 
ACAGCCACAT TGACTACCAC ACCATTGGCC CCAATGCTGC CTATAGCCAC GGTTACACCG 
GCTCCAGCGA CCAATAATAG TAACGTTAGC TCTCTTACTG GAGTTCAGTT GCGAAACCCT 
CAACGGGATG CGACAGCAAG TGAATCTGAT GCGGATTATG TAAACACACC AAAGCCGCGT 
TCGAGCCAAA ATGAAGTTAA TCGCACTATG TCCATAAGAA GCACTGGCGA ACCCATTCAG 
AAGTATTCGG CGAATGGGAC GGAAGCAAAC GACGTTACTT TACGACACTC TCAGTCGACT 
AGGGAATCGG TTAGACCAGG ATCTACTGGG GAGGAGCGAA ACTCCACGTA TGGTATGGTT 
GGAGGTAACT CCAAACGCGA CAGCGCCAGC ATTGTCTGCT CGAACAACTC GAACAACACG 
CGCACCCTTC TGATGCAGAG TCCCTTGGTG GACCCTACGG CCATTCAGGT CAGCATTAGT 
CCCGCTCACA CAGCGGAACC TGTGTTGACA CCAGGAGAGA AGTTACGCCG CTTGGACGCC 
TCCATTAGGA ATGATTTGCT GGAGAAGCAG AAAATCATTT GTGATATCTT CCGTTTGCCA 
GTGGAACACT ACGACCAGAT TGTGGACATT GCCATGATGC CAGAGGCGCC GAAAGACAGT 
GCAGATATTG CTTTAGCTGC TTACGATCAG ATTCAAACCC TGACCAAGAT GCTGAACGAG 
TACATGCACG TCACGCCTGA GCAAGAGGTC TCAGCGGTGT CCACGGCGGT TTGTGGCCAC 
TGTCACGAGA AGGAAAAGCT TCGTAAGAAG GTGGCACCAT CCTCATCGTT TTCTTCATCA 
CCACCACCGC TGCCGCCTCC CAATAGGCAG CATGCCCAGG CTCAGGCCCA GATACCGCCA 
TCGCGGCTGA TGCCCAAACT ACAAACTCTT GATCTTGACG AAGTTGCCAT ACACGAAGAC 
GATGACGGAT ACTGTGAGAT CGACGAACTG CGCTTACCGG CTATTCCGTC CAAACCACAT 
GAGCGGCCCA CAACGCCACT GGCTCCTTTC AATACTGAGC CGAAAACTTC ACAATCTGTT 
ATAGATGCCT CGAAACGTCA ATCCACTGAT GCCGTTCCGG AGGGATTACT GGAACAAGAA 
CCACTCGAAG GCGATAAGAC GGAGACCAAG GGTGAAGATA ATGAAGTGAA AACTGTGCCG 
TCAGATAAGC TAAGTGAATC ATGCAATGAA GAGAGGCAAT GTGTGGAGGC GGATATCACA 
AAGGAAGTGG CAGATCCAAC GACCTCTAAG AATGAAGCTG CAGCATCGGT GGATGAATTA 
CCAAGCCAGA GCCGGGAGAT AAAAACGGCT GAAAACGCAA GCAAATCTGT AGCTGACAAA 
AAGGAAGACA ACGAGGAAAC CATCGAAGAA GGTGTGGCAT CCACGGTCGA TAGCTCCACT 
CAAACATCAC CAACTGAATC GCCGAAAGAG ACGGATAAGT TAACTGGAGG ATCGAGCAGC 
ACCTGTGGGC CGAATCGCAT TCAGCACGCT AGTGTGCTGG AGCCGAGTGT GCCCTGCCAT 
GCACTCAGCA GCATTGTAAC AATACTGAAT GAGCAGATTT CCATGCTTTT GCCAAAAATT 
AACGAACGCG ATATGGAAAG GGAGCGATTG CGTAAAGAGA ATCAACACCT TCGCGAGCTC 
TTGAGTGCGC TGCATGATCG ACAGCGAGTT GATGAAGTAA AGGAAACTCC GTTTGATCTA 
AAGAAGCTGA TGCATGCTGA GGATGTAGAG TTTGACGATG ATATTGACGC CATTTCCAAC 
AGTTCGCTGA CGCCAACGCC TACGCCGATT CCCACAGCAT CACCAAGCGC CAGCGGCCAG 
GTAGAGACAG CGGAAGCCAT GAGGATTACT AGCACTGAGG ATGAGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGEF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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