Rho1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rho1
Entrez Gene ID	36775
Full Name	CG8416 gene product from transcript CG8416-RB
Synonyms	19549712,8416,AAF01186,CG8416,D-Rho1,DRho,DRho1,DRhoA,Dm Rho1,DmRHO-A,Dmel\CG8416,Drho1,DrhoA,E3.10/J3.8,P48148,RHO,RHO1,Rho,Rho A,Rho GTPase,Rho kinase,RhoA,RhoN19,dRho1,dRhoA,dnRho,l(2)52Fa,l(2)k02107b,n(2)k07236,rho,rho1,rhoA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E5-52E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_057750.4	NP_477098.1	Rho1, isoform A
NM_134308.3	NP_599135.1	Rho1, isoform B
NM_166139.3	NP_725524.1	Rho1, isoform D
NM_134309.2	NP_599136.1	Rho1, isoform C
NM_206128.2	NP_995850.1	Rho1, isoform F
NM_206127.2	NP_995849.1	Rho1, isoform G

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RHOC	XP_540336.2
Danio rerio (zebrafish)	rhoad	NP_001002445.1
Danio rerio (zebrafish)	rhoac	NP_998515.1
Danio rerio (zebrafish)	rhoae	NP_957444.1
Bos taurus (cattle)	RHOC	NP_001039603.1
Xenopus tropicalis (tropical clawed frog)	rhoc	NP_001016569.1
Homo sapiens (human)	RHOC	NP_001036143.1
Pan troglodytes (chimpanzee)	RHOC	XP_003339043.1
Mus musculus (house mouse)	Rhoc	NP_031510.2
Rattus norvegicus (Norway rat)	Rhoc	NP_001099931.1
Gallus gallus (chicken)	RHOC	NP_001025020.1
Danio rerio (zebrafish)	rhoaa	NP_998302.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005160	XP_001688510.1
Caenorhabditis elegans (roundworm)	rho-1	NP_502959.1
Eremothecium gossypii	AGOS_ABR182W	NP_983130.1
Drosophila melanogaster (fruit fly)	Rho1	NP_995851.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis
Title: Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis.

Study identified circadian rhythms in Rho1 GTPase activity in s-LNv axons that are regulated by rhythmic transcription of Pura, a Rho1 GEF. Pura activates Rho1 to retract s-LNv axons, decrease active zone numbers and reduce s-LNvs' influence on the clock network. Thus, study links transcription, plasticity, network hierarchy and behavior.
Title: Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy.

Developmental hemocyte migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein.
Title: Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.

impaired Hippo signaling induces JNK activation through Rho1.
Title: Impaired Hippo signaling promotes Rho1-JNK-dependent growth.

This work identifies spatially distinct functions for Rho1 in the regulation of DE-cadherin-containing vesicular trafficking during adherens junctions remodeling in live epithelia.
Title: Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II.

The following Rho1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rho1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10163	NM_134308.3 Latest version!	Drosophila melanogaster Rho1, transcript variant B (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10163	NM_206128.2 Latest version!	Drosophila melanogaster Rho1, transcript variant F (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10163	NM_206127.2 Latest version!	Drosophila melanogaster Rho1, transcript variant G (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10163	NM_166139.3 Latest version!	Drosophila melanogaster Rho1, transcript variant D (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10163	NM_134309.2 Latest version!	Drosophila melanogaster Rho1, transcript variant C (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10163	NM_057750.4 Latest version!	Drosophila melanogaster Rho1, transcript variant A (Rho1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rho1 ( NM_057750.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477098.1 Drosophila melanogaster Rho1, transcript variant A (Rho1), mRNA.

Price & Availability↑

CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_057750.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057750.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477098.1
CDS	272..850
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_057750.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant A (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057750.4

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rho1 ( NM_134308.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599135.1 Drosophila melanogaster Rho1, transcript variant B (Rho1), mRNA.

Price & Availability↑

CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_134308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_134308.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599135.1
CDS	272..850
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_134308.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant B (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134308.3

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rho1 ( NM_166139.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725524.1 Drosophila melanogaster Rho1, transcript variant D (Rho1), mRNA.

Price & Availability↑

CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_166139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166139.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725524.1
CDS	154..732
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_166139.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant D (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166139.3

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rho1 ( NM_134309.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599136.1 Drosophila melanogaster Rho1, transcript variant C (Rho1), mRNA.

Price & Availability↑

CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_134309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_134309.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599136.1
CDS	143..721
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_134309.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant C (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134309.2

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rho1 ( NM_206128.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995850.1 Drosophila melanogaster Rho1, transcript variant F (Rho1), mRNA.

Price & Availability↑

CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_206128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206128.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995850.1
CDS	215..793
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_206128.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant F (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206128.2

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rho1 ( NM_206127.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995849.1 Drosophila melanogaster Rho1, transcript variant G (Rho1), mRNA.

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CloneID	OFa10163
Clone ID Related Accession (Same CDS sequence)	NM_057750.4 , NM_134308.3 , NM_166139.3 , NM_134309.2 , NM_206128.2 , NM_206127.2
Accession Version	NM_206127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206127.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995849.1
CDS	221..799
Translation	MTTIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPNTIRDLAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVRDVFETATRAALQVKKRKKTRCLLL

Target ORF information:

RefSeq Version	NM_206127.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho1, transcript variant G (Rho1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206127.2

ORF Insert Sequence:

ATGACGACGA TTCGCAAGAA ATTGGTAATT GTCGGCGACG GTGCCTGCGG TAAAACTTGC 
CTTCTGATTG TCTTCAGCAA AGATCAGTTC CCCGAGGTCT ATGTGCCCAC CGTATTCGAG 
AATTATGTGG CCGACATCGA GGTGGATGGC AAACAGGTGG AGCTGGCCTT GTGGGATACG 
GCCGGGCAGG AGGACTACGA CAGACTACGA CCGCTGAGCT ATCCCGACAC TGACGTCATA 
CTGATGTGTT TCTCAGTGGA TTCACCCGAT TCGCTAGAAA ATATTCCTGA AAAATGGACC 
CCAGAGGTCA AACACTTTTG TCCAAATGTT CCAATCATTT TGGTAGGAAA TAAGAAAGAT 
TTGCGAAATG ATCCCAACAC AATTCGGGAT CTAGCAAAAA TGAAGCAGGA GCCGGTGAAG 
CCGCAGGAGG GTCGCGCCAT GGCCGAGAAG ATTAATGCCT TTGCCTATTT GGAGTGTTCG 
GCTAAGTCCA AGGAGGGTGT GCGAGATGTT TTCGAGACGG CAACTAGGGC CGCGCTGCAA 
GTCAAAAAGA GGAAGAAGAC CAGATGCCTT TTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rho1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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