unc-5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

  • Gene

  • Clones

  • gRNAs

The following unc-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa10086 NM_001299521.1
Latest version!
Drosophila melanogaster unc-5, transcript variant B (unc-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa10086 NM_137189.3
Latest version!
Drosophila melanogaster unc-5, transcript variant A (unc-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa10086
    Clone ID Related Accession (Same CDS sequence) NM_001299521.1 , NM_137189.3
    Accession Version NM_001299521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product unc-5, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
    ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG
    TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC
    TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC
    AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG
    GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC
    GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC
    AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG
    TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG
    GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC
    GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT
    CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG
    CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC
    GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG
    ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC
    TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC
    GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC
    AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG
    GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG
    GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC
    TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG
    AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG
    CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA
    GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA
    TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT
    GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC
    TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT
    TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG
    GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT
    CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG
    CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC
    GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC
    GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG
    GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG
    CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC
    AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC
    CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT
    CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC
    AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG
    CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG
    GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA
    CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC
    AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG
    GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC
    GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC
    ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT
    GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG
    TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC
    TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG
    ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG
    AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG
    ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT
    TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA
    GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286450.1
    CDS872..4090
    Translation

    Target ORF information:

    RefSeq Version NM_001299521.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster unc-5, transcript variant B (unc-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
    ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG
    TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC
    TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC
    AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG
    GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC
    GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC
    AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG
    TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG
    GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC
    GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT
    CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG
    CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC
    GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG
    ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC
    TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC
    GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC
    AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG
    GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG
    GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC
    TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG
    AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG
    CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA
    GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA
    TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT
    GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC
    TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT
    TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG
    GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT
    CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG
    CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC
    GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC
    GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG
    GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG
    CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC
    AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC
    CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT
    CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC
    AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG
    CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG
    GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA
    CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC
    AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG
    GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC
    GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC
    ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT
    GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG
    TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC
    TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG
    ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG
    AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG
    ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT
    TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA
    GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa10086
    Clone ID Related Accession (Same CDS sequence) NM_001299521.1 , NM_137189.3
    Accession Version NM_137189.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product unc-5, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
    ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG
    TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC
    TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC
    AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG
    GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC
    GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC
    AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG
    TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG
    GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC
    GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT
    CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG
    CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC
    GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG
    ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC
    TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC
    GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC
    AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG
    GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG
    GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC
    TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG
    AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG
    CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA
    GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA
    TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT
    GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC
    TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT
    TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG
    GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT
    CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG
    CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC
    GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC
    GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG
    GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG
    CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC
    AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC
    CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT
    CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC
    AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG
    CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG
    GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA
    CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC
    AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG
    GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC
    GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC
    ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT
    GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG
    TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC
    TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG
    ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG
    AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG
    ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT
    TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA
    GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_611033.2
    CDS670..3888
    Translation

    Target ORF information:

    RefSeq Version NM_137189.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster unc-5, transcript variant A (unc-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_137189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
    ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG
    TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC
    TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC
    AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG
    GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC
    GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC
    AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG
    TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG
    GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC
    GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT
    CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG
    CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC
    GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG
    ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC
    TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC
    GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC
    AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG
    GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG
    GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC
    TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG
    AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG
    CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA
    GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA
    TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT
    GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC
    TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT
    TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG
    GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT
    CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG
    CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC
    GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC
    GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG
    GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG
    CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC
    AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC
    CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT
    CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC
    AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG
    CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG
    GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA
    CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC
    AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG
    GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC
    GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC
    ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT
    GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG
    TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC
    TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG
    ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG
    AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG
    ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT
    TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA
    GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.