unc-5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-5
Entrez Gene ID	36703
Full Name	CG8166 gene product from transcript CG8166-RB
Synonyms	AAF7419,CG8166,CG8168,CT20824,Dmel\CG8166,Dunc5,UNC-5,UNC5,UNC5D,Unc-5,Unc5,Unc5d,dUnc5,unc5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51F7-51F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299521.1	NP_001286450.1	unc-5, isoform B
NM_137189.3	NP_611033.2	unc-5, isoform A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	unc5c	XP_004911160.1
Pan troglodytes (chimpanzee)	UNC5C	XP_526636.2
	UNC5C	XP_002804202.1
Gallus gallus (chicken)	UNC5C	NP_989782.1
Danio rerio (zebrafish)	unc5c	NP_001093454.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009061	XP_319812.4
Homo sapiens (human)	UNC5C	NP_003719.3
Canis lupus familiaris (dog)	UNC5C	XP_005639270.1
Bos taurus (cattle)	UNC5C	XP_005207793.1
Rattus norvegicus (Norway rat)	Unc5c	NP_955439.1
Drosophila melanogaster (fruit fly)	unc-5	NP_611033.2
Mus musculus (house mouse)	Unc5c	NP_033498.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data strongly suggests that the eve-grn transcriptional code controls axon guidance, in part, by regulating the level of unc-5 expression.
Title: A GATA/homeodomain transcriptional code regulates axon guidance through the Unc-5 receptor.

these findings support the idea that Unc5 is crucial for lumen formation and thereby represents a repulsive cue acting during Drosophila heart tube formation.
Title: The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.

The following unc-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10086	NM_001299521.1 Latest version!	Drosophila melanogaster unc-5, transcript variant B (unc-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10086	NM_137189.3 Latest version!	Drosophila melanogaster unc-5, transcript variant A (unc-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-5 ( NM_001299521.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286450.1 Drosophila melanogaster unc-5, transcript variant B (unc-5), mRNA.

Price & Availability↑

CloneID	OFa10086
Clone ID Related Accession (Same CDS sequence)	NM_001299521.1 , NM_137189.3
Accession Version	NM_001299521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG 
TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC 
TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC 
AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG 
GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC 
GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC 
AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG 
TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG 
GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC 
GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT 
CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG 
CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC 
GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG 
ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC 
TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC 
GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC 
AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG 
GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG 
GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC 
TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG 
AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG 
CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA 
GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA 
TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT 
GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC 
TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT 
TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG 
GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT 
CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG 
CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC 
GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC 
GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG 
GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG 
CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC 
AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC 
CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT 
CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC 
AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG 
CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG 
GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA 
CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC 
AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG 
GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC 
GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC 
ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT 
GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG 
TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC 
TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG 
ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG 
AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG 
ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT 
TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA 
GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286450.1
CDS	872..4090
Translation	MAVINKAGNVIALLLVKLQLILLFTLSVSGELPQLDYGSLSASLEEDAIDPLTAMAPFANSLVTEESAQHKNNAELLGNSSEDENVRPQQGSSSSGLGSSGAAGGSGILLEEFNSGKLSPGEASNTLPIFLIEPESVFVVKNRPAVLKCKASHSLQVIFKCSGSSQPPPSTHETHVDPHTGVNMEEVTATIHRDLVDEFFGDGPFKCECHAWSSRGVVKSQAATVHIAYIRKSFNQSPTSLRLELGSRAELRCEPPGGFPEPKLTWHKNNAVITADSEPGITVSAGTLIFRQVALQHMANYSCSAENIAGRRVSDSAVLIVYVNGGWSTWSPWRECKCAGKPSQGRKRSRTCNNPMPLNGGAQCPGPQIQKSADCAACPEDTQIVSPDGFDISSSKRMARWSAWSDWSICSAECIQVRRRKCLTQGQTQISSEAEEAGDLLLGAPGVGMAALIAAAGVGAVGSPSEATGSSSDIIPGYGKSLCAGKDIQTAECRGEQCQIGKDDFDWTLYLGLAFITAVCFAFGTALICCARRGIRTNPHYNMARSVMDADYMPGVVEKKEMRMHIEASNMGYDYVNPGHRYLPGEHIMGMGIGCGGVTEHHYDVPNLSANYTNPIDHLSVDYLSETGESSTADTSNSTFDMNGKLSILNASKSSTYEMLGSAGGQLRLYGGELLLFVPEHAIGKHVKKHVSLLLLSDECSRVSCATESSILCSSVVHSAPRNYSFVKPVILKIPHCLVAPEQWHVHIYHADSEHDELSVNWRRAVSVGEETINTPMFVQLEATHVFIMTEQLGHFTVVAEPRIQQPSIKMKLLAFSQHTPPSNANCSLRIYVVKDFPNSRDICANVEAKLGGSFLGESQVFAFTLNSRNLNIRVRSADVEAAAPYEHAIPYQHILSNNSILHCEFSLRRQDQNSLCVDFGQGSEDDYYTFNIPAHSMSGSAEELASTTNTTISIDRQGNYVNESCVMDFVQLPHATKRLICGALDPPRADERDWRLLAKKLNTDRYIAYFATKASPTEQILNLWECRANSSPGSSSNSVSHTIMALLLTLKEMGRQDVLDIIVETIGPLWI

Target ORF information:

RefSeq Version	NM_001299521.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-5, transcript variant B (unc-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299521.1

ORF Insert Sequence:

ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG 
TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC 
TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC 
AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG 
GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC 
GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC 
AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG 
TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG 
GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC 
GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT 
CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG 
CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC 
GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG 
ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC 
TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC 
GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC 
AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG 
GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG 
GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC 
TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG 
AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG 
CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA 
GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA 
TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT 
GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC 
TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT 
TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG 
GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT 
CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG 
CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC 
GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC 
GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG 
GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG 
CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC 
AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC 
CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT 
CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC 
AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG 
CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG 
GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA 
CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC 
AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG 
GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC 
GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC 
ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT 
GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG 
TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC 
TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG 
ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG 
AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG 
ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT 
TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA 
GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-5 ( NM_137189.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611033.2 Drosophila melanogaster unc-5, transcript variant A (unc-5), mRNA.

Price & Availability↑

CloneID	OFa10086
Clone ID Related Accession (Same CDS sequence)	NM_001299521.1 , NM_137189.3
Accession Version	NM_137189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG 
TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC 
TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC 
AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG 
GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC 
GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC 
AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG 
TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG 
GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC 
GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT 
CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG 
CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC 
GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG 
ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC 
TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC 
GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC 
AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG 
GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG 
GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC 
TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG 
AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG 
CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA 
GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA 
TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT 
GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC 
TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT 
TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG 
GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT 
CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG 
CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC 
GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC 
GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG 
GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG 
CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC 
AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC 
CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT 
CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC 
AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG 
CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG 
GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA 
CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC 
AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG 
GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC 
GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC 
ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT 
GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG 
TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC 
TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG 
ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG 
AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG 
ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT 
TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA 
GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611033.2
CDS	670..3888
Translation	MAVINKAGNVIALLLVKLQLILLFTLSVSGELPQLDYGSLSASLEEDAIDPLTAMAPFANSLVTEESAQHKNNAELLGNSSEDENVRPQQGSSSSGLGSSGAAGGSGILLEEFNSGKLSPGEASNTLPIFLIEPESVFVVKNRPAVLKCKASHSLQVIFKCSGSSQPPPSTHETHVDPHTGVNMEEVTATIHRDLVDEFFGDGPFKCECHAWSSRGVVKSQAATVHIAYIRKSFNQSPTSLRLELGSRAELRCEPPGGFPEPKLTWHKNNAVITADSEPGITVSAGTLIFRQVALQHMANYSCSAENIAGRRVSDSAVLIVYVNGGWSTWSPWRECKCAGKPSQGRKRSRTCNNPMPLNGGAQCPGPQIQKSADCAACPEDTQIVSPDGFDISSSKRMARWSAWSDWSICSAECIQVRRRKCLTQGQTQISSEAEEAGDLLLGAPGVGMAALIAAAGVGAVGSPSEATGSSSDIIPGYGKSLCAGKDIQTAECRGEQCQIGKDDFDWTLYLGLAFITAVCFAFGTALICCARRGIRTNPHYNMARSVMDADYMPGVVEKKEMRMHIEASNMGYDYVNPGHRYLPGEHIMGMGIGCGGVTEHHYDVPNLSANYTNPIDHLSVDYLSETGESSTADTSNSTFDMNGKLSILNASKSSTYEMLGSAGGQLRLYGGELLLFVPEHAIGKHVKKHVSLLLLSDECSRVSCATESSILCSSVVHSAPRNYSFVKPVILKIPHCLVAPEQWHVHIYHADSEHDELSVNWRRAVSVGEETINTPMFVQLEATHVFIMTEQLGHFTVVAEPRIQQPSIKMKLLAFSQHTPPSNANCSLRIYVVKDFPNSRDICANVEAKLGGSFLGESQVFAFTLNSRNLNIRVRSADVEAAAPYEHAIPYQHILSNNSILHCEFSLRRQDQNSLCVDFGQGSEDDYYTFNIPAHSMSGSAEELASTTNTTISIDRQGNYVNESCVMDFVQLPHATKRLICGALDPPRADERDWRLLAKKLNTDRYIAYFATKASPTEQILNLWECRANSSPGSSSNSVSHTIMALLLTLKEMGRQDVLDIIVETIGPLWI

Target ORF information:

RefSeq Version	NM_137189.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-5, transcript variant A (unc-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137189.3

ORF Insert Sequence:

ATGGCGGTGA TTAATAAAGC CGGAAATGTG ATTGCCCTCC TGCTGGTTAA GCTTCAGCTA 
ATCCTGCTGT TCACGCTGTC AGTTTCCGGA GAGTTGCCGC AGCTGGACTA CGGCAGCTTG 
TCCGCATCCC TGGAGGAGGA TGCTATTGAC CCACTCACGG CGATGGCACC CTTCGCCAAC 
TCCCTGGTCA CCGAAGAATC GGCGCAGCAC AAGAACAACG CCGAGCTGCT TGGCAATAGC 
AGTGAGGATG AGAATGTGAG ACCGCAGCAA GGGTCCTCTT CCTCGGGACT AGGATCATCG 
GGAGCAGCAG GTGGATCAGG CATACTGCTC GAGGAGTTTA ACAGCGGCAA ACTGAGTCCC 
GGAGAGGCGA GTAATACGCT GCCCATATTC CTCATCGAGC CGGAGAGCGT GTTCGTGGTC 
AAGAACCGGC CGGCGGTGCT TAAGTGCAAG GCCTCCCACT CGCTGCAGGT GATCTTCAAG 
TGTAGCGGCA GTTCGCAACC ACCGCCGTCC ACGCACGAAA CTCACGTGGA TCCGCACACG 
GGGGTCAACA TGGAGGAGGT CACCGCCACC ATCCACCGCG ACCTGGTCGA CGAGTTCTTC 
GGCGACGGAC CCTTCAAGTG CGAGTGCCAC GCGTGGTCGT CGCGTGGCGT GGTCAAGAGT 
CAGGCGGCCA CCGTGCACAT TGCTTATATT CGCAAGTCCT TCAACCAGTC GCCCACCTCG 
CTGCGCCTGG AGCTGGGCAG TCGGGCGGAA CTGCGCTGCG AACCACCCGG CGGTTTCCCC 
GAACCGAAGC TCACCTGGCA CAAAAACAAC GCGGTCATCA CGGCGGACAG CGAGCCGGGG 
ATCACCGTTT CGGCCGGCAC ACTCATCTTC CGCCAGGTGG CCCTGCAGCA TATGGCCAAC 
TATAGCTGCA GTGCGGAGAA TATCGCTGGC AGACGCGTCT CCGATTCCGC CGTGCTCATC 
GTTTATGTCA ACGGTGGCTG GAGCACCTGG AGTCCATGGC GCGAATGCAA GTGTGCGGGC 
AAGCCCAGCC AGGGAAGGAA GCGTTCGCGC ACCTGCAACA ATCCGATGCC ATTGAATGGG 
GGCGCCCAGT GTCCGGGCCC CCAGATCCAG AAGTCCGCCG ACTGCGCCGC ATGTCCAGAG 
GACACTCAAA TCGTGAGCCC TGATGGATTT GACATTTCGT CGAGTAAGCG CATGGCCCGC 
TGGTCGGCGT GGAGTGACTG GAGCATCTGC TCCGCGGAGT GCATTCAAGT GCGTCGCCGG 
AAGTGCCTGA CTCAGGGCCA GACTCAGATT TCCTCCGAGG CGGAGGAAGC CGGTGATCTG 
CTCCTGGGAG CTCCCGGAGT GGGCATGGCC GCCCTCATCG CCGCTGCAGG AGTGGGCGCA 
GTAGGAAGCC CCAGCGAAGC GACTGGCTCC AGTTCCGACA TTATCCCGGG ATATGGCAAA 
TCATTGTGCG CCGGAAAAGA CATACAAACG GCCGAATGCC GCGGCGAACA GTGCCAGATT 
GGCAAGGATG ATTTCGATTG GACCCTCTAT CTGGGATTGG CCTTCATAAC GGCGGTGTGC 
TTCGCCTTCG GGACCGCACT GATCTGCTGC GCTCGCCGTG GCATCCGCAC CAATCCCCAT 
TACAACATGG CCCGTTCAGT AATGGACGCG GATTATATGC CCGGCGTTGT GGAGAAGAAG 
GAGATGCGCA TGCACATCGA GGCCAGCAAC ATGGGATACG ATTACGTCAA TCCCGGACAT 
CGTTATTTGC CCGGCGAGCA CATCATGGGC ATGGGCATCG GTTGTGGAGG CGTCACCGAG 
CACCATTACG ACGTGCCCAA TCTGTCCGCC AACTACACTA ATCCAATAGA TCATCTAAGC 
GTGGACTATT TGTCAGAGAC CGGGGAATCC TCCACAGCTG ATACATCGAA CTCAACCTTC 
GATATGAACG GCAAGCTGTC CATACTCAAT GCCTCAAAGA GCAGCACATA TGAGATGCTG 
GGAAGCGCAG GAGGACAGCT CCGCCTCTAT GGCGGCGAGC TGCTGCTCTT TGTCCCGGAG 
CACGCCATTG GAAAGCACGT CAAGAAGCAC GTATCCCTGC TGCTGCTGAG CGACGAGTGC 
AGTCGCGTGT CCTGCGCCAC CGAGAGCTCC ATTCTGTGCA GCAGTGTGGT TCATAGTGCC 
CCGCGGAACT ACAGCTTCGT GAAACCAGTG ATCCTGAAGA TTCCCCACTG CCTGGTGGCT 
CCGGAGCAGT GGCATGTTCA CATCTATCAC GCGGACAGCG AACACGACGA GCTGAGTGTC 
AACTGGAGGC GGGCAGTTTC TGTCGGCGAG GAAACCATCA ACACACCCAT GTTCGTACAG 
CTGGAGGCGA CGCATGTGTT CATTATGACT GAGCAACTGG GTCACTTCAC CGTGGTGGCG 
GAGCCGAGGA TTCAACAACC CTCGATCAAG ATGAAGCTGC TGGCCTTCAG TCAACATACA 
CCACCCAGCA ACGCAAATTG CAGTTTGCGG ATTTACGTGG TCAAGGACTT CCCCAATAGC 
AGGGATATCT GCGCCAATGT GGAGGCCAAG CTGGGTGGCA GTTTTCTTGG CGAGAGCCAG 
GTCTTTGCGT TTACCCTGAA TAGCCGAAAT CTAAACATCC GGGTGAGAAG CGCGGATGTC 
GAGGCAGCAG CTCCCTACGA ACATGCCATA CCCTACCAGC ACATCCTCAG CAACAACTCC 
ATTCTGCACT GCGAGTTTAG CTTGAGGAGG CAGGATCAGA ACAGCCTGTG CGTGGACTTT 
GGCCAGGGCA GCGAGGACGA CTACTACACC TTTAACATTC CAGCGCACTC TATGAGCGGG 
TCGGCAGAGG AACTGGCATC CACCACAAAC ACCACCATCT CAATTGACCG CCAGGGCAAC 
TATGTAAACG AAAGCTGTGT GATGGACTTC GTCCAGCTGC CGCACGCCAC GAAGCGTTTG 
ATCTGCGGCG CCTTGGATCC TCCACGGGCG GACGAGCGGG ATTGGCGATT ACTGGCCAAG 
AAGCTGAATA CCGACCGATA TATTGCGTAC TTTGCCACCA AAGCCTCGCC CACCGAACAG 
ATCCTGAATC TGTGGGAGTG CCGAGCGAAT AGCAGTCCTG GTAGTAGTTC CAACTCAGTT 
TCACATACGA TAATGGCACT CCTGCTGACG CTTAAGGAGA TGGGACGTCA GGATGTGCTA 
GACATCATAG TTGAAACCAT AGGCCCTTTG TGGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

unc-5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報