PI31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI31
Entrez Gene ID	36277
Full Name	Proteasome inhibitor 31 kDa
Synonyms	8979,CG8979,DmPI31,Dmel\CG8979,cg8979,dmPI31
General protein information	Preferred Names Proteasome inhibitor 31 kDa Names CG8979 gene product from transcript CG8979-RD CG8979-PA CG8979-PB CG8979-PC CG8979-PD CG8979-PF PI31-PA PI31-PB PI31-PC PI31-PD PI31-PF proteasome inhibitor 31 kDa
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48D5-48D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_165855.3	NP_725093.1	proteasome inhibitor 31 kDa, isoform C
NM_001273978.2	NP_001260907.1	proteasome inhibitor 31 kDa, isoform F
NM_136871.4	NP_610715.1	proteasome inhibitor 31 kDa, isoform A
NM_165854.3	NP_725092.1	proteasome inhibitor 31 kDa, isoform B
NM_001273976.2	NP_001260905.1	proteasome inhibitor 31 kDa, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSMF1	NP_848693.2
Bos taurus (cattle)	PSMF1	NP_001069946.1
	PSMF1	XP_001112708.1
Canis lupus familiaris (dog)	PSMF1	XP_534374.2
Rattus norvegicus (Norway rat)	Psmf1	NP_001094475.1
Danio rerio (zebrafish)	psmf1	NP_001002547.2
Drosophila melanogaster (fruit fly)	PI31	NP_725093.1
Mus musculus (house mouse)	Psmf1	NP_997611.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007418	XP_308420.3
Pan troglodytes (chimpanzee)	PSMF1	XP_001152935.1
Gallus gallus (chicken)	PSMF1	NP_001026753.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified DmPI31 as a binding partner of the F box protein Nutcracker, a component of an SCF ubiquitin ligase (E3) required for caspase activation during sperm differentiation in Drosophila.
Title: A conserved F box regulatory complex controls proteasome activity in Drosophila.

The following PI31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09660	NM_165854.3 Latest version!	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant B (PI31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09660	NM_001273976.2 Latest version!	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant D (PI31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09660	NM_136871.4 Latest version!	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant A (PI31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09660	NM_001273978.2 Latest version!	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant F (PI31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09660	NM_165855.3 Latest version!	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant C (PI31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI31 ( NM_165855.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725093.1 Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant C (PI31), mRNA.

Price & Availability↑

CloneID	OFa09660
Clone ID Related Accession (Same CDS sequence)	NM_165855.3 , NM_001273978.2 , NM_136871.4 , NM_165854.3 , NM_001273976.2
Accession Version	NM_165855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome inhibitor 31 kDa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165855.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725093.1
CDS	137..949
Translation	MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Target ORF information:

RefSeq Version	NM_165855.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant C (PI31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165855.3

ORF Insert Sequence:

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI31 ( NM_001273978.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260907.1 Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant F (PI31), mRNA.

Price & Availability↑

CloneID	OFa09660
Clone ID Related Accession (Same CDS sequence)	NM_165855.3 , NM_001273978.2 , NM_136871.4 , NM_165854.3 , NM_001273976.2
Accession Version	NM_001273978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome inhibitor 31 kDa, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273978.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260907.1
CDS	173..985
Translation	MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Target ORF information:

RefSeq Version	NM_001273978.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant F (PI31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273978.2

ORF Insert Sequence:

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI31 ( NM_136871.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610715.1 Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant A (PI31), mRNA.

Price & Availability↑

CloneID	OFa09660
Clone ID Related Accession (Same CDS sequence)	NM_165855.3 , NM_001273978.2 , NM_136871.4 , NM_165854.3 , NM_001273976.2
Accession Version	NM_136871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome inhibitor 31 kDa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136871.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610715.1
CDS	140..952
Translation	MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Target ORF information:

RefSeq Version	NM_136871.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant A (PI31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136871.4

ORF Insert Sequence:

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI31 ( NM_165854.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725092.1 Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant B (PI31), mRNA.

Price & Availability↑

CloneID	OFa09660
Clone ID Related Accession (Same CDS sequence)	NM_165855.3 , NM_001273978.2 , NM_136871.4 , NM_165854.3 , NM_001273976.2
Accession Version	NM_165854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome inhibitor 31 kDa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165854.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725092.1
CDS	204..1016
Translation	MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Target ORF information:

RefSeq Version	NM_165854.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant B (PI31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165854.3

ORF Insert Sequence:

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI31 ( NM_001273976.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260905.1 Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant D (PI31), mRNA.

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CloneID	OFa09660
Clone ID Related Accession (Same CDS sequence)	NM_165855.3 , NM_001273978.2 , NM_136871.4 , NM_165854.3 , NM_001273976.2
Accession Version	NM_001273976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome inhibitor 31 kDa, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273976.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260905.1
CDS	119..931
Translation	MESGSTAKTGDFFYGWDLLYKTVKADVSKKSDLLIALVHFLLTKHYNFRCVGVGDDKTLPEEEGSELLPDSWNDDDTKYSLRYVHDKMLYLLLGHITEGSLLINLLDINTKKVSNICVEPETLVPEVKGGITTIMPSASEIVERYRRELLDPVFTGNSREVTTQTTNSPRPIGSDPDPLRIGEPRRGGSFIPSAFEPRPFGFPDVGRGDLDPLGRGGHGNLFSFPSRPNMGPGPVPRFDPFNPLNPNRPGQGGINPDHMRPPDWNPDYYM

Target ORF information:

RefSeq Version	NM_001273976.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome inhibitor 31 kDa, transcript variant D (PI31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273976.2

ORF Insert Sequence:

ATGGAAAGTG GGTCGACTGC CAAGACGGGC GATTTCTTTT ACGGTTGGGA TCTGCTGTAC 
AAGACCGTTA AGGCTGATGT CTCCAAGAAG TCAGACCTTT TGATTGCCCT CGTCCATTTT 
CTTTTGACCA AGCATTACAA CTTCCGATGC GTTGGCGTTG GCGATGATAA AACCTTGCCG 
GAGGAGGAGG GCAGTGAGCT GCTGCCGGAC AGCTGGAATG ATGACGATAC CAAGTACTCG 
CTGCGCTACG TGCACGATAA GATGCTGTAT TTGCTGCTGG GCCACATCAC CGAGGGCTCC 
CTGCTGATCA ACCTGTTGGA CATCAACACT AAGAAGGTAT CCAACATCTG CGTAGAACCA 
GAGACCCTGG TACCTGAGGT CAAGGGAGGC ATCACCACCA TAATGCCGAG TGCCTCAGAG 
ATTGTTGAAC GCTACCGTAG GGAGCTTCTA GACCCTGTAT TCACGGGAAA CTCGCGCGAA 
GTTACCACGC AGACGACCAA CTCACCACGC CCCATTGGAT CGGATCCAGA TCCCTTGCGC 
ATCGGCGAGC CAAGGCGTGG CGGATCCTTC ATTCCAAGTG CATTCGAGCC TCGCCCTTTT 
GGATTTCCGG ACGTTGGACG CGGGGATCTC GATCCACTGG GTCGCGGTGG TCATGGTAAC 
CTGTTCAGCT TTCCATCACG CCCAAATATG GGCCCGGGAC CCGTGCCCCG CTTCGACCCG 
TTCAATCCCC TGAACCCGAA TAGGCCGGGA CAGGGTGGAA TCAATCCAGA TCATATGCGC 
CCACCAGATT GGAATCCCGA TTACTACATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PI31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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