Psmf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psmf1
Entrez Gene ID	228769
Full Name	proteasome (prosome, macropain) inhibitor subunit 1
Synonyms	AW048666,BC012260,PI31
General protein information	Preferred Names proteasome (prosome, macropain) inhibitor subunit 1 Names proteasome inhibitor PI31 subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_212446.2	NP_997611.1	proteasome inhibitor PI31 subunit isoform 1
XM_006499306.4	XP_006499369.1	proteasome inhibitor PI31 subunit isoform X1
XM_006499306.3	XP_006499369.1	proteasome inhibitor PI31 subunit isoform X1
NM_001305244.1	NP_001292173.1	proteasome inhibitor PI31 subunit isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSMF1	NP_848693.2
Bos taurus (cattle)	PSMF1	NP_001069946.1
	PSMF1	XP_001112708.1
Canis lupus familiaris (dog)	PSMF1	XP_534374.2
Rattus norvegicus (Norway rat)	Psmf1	NP_001094475.1
Danio rerio (zebrafish)	psmf1	NP_001002547.2
Drosophila melanogaster (fruit fly)	PI31	NP_725093.1
Mus musculus (house mouse)	Psmf1	NP_997611.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007418	XP_308420.3
Pan troglodytes (chimpanzee)	PSMF1	XP_001152935.1
Gallus gallus (chicken)	PSMF1	NP_001026753.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

PI31 is a modulator of proteasome formation and antigen processing.
Zaiss DM, Standera S, Kloetzel PM, Sijts AJ
Proceedings of the National Academy of Sciences of the United States of America99(22)14344-9(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PI31 acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing
Title: PI31 is a modulator of proteasome formation and antigen processing.

The following Psmf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psmf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73419	NM_001305244.1 Latest version!	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu14676	NM_212446.2 Latest version!	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu30340	XM_006499306.4 Latest version!	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu30340	XM_006499306.3	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psmf1 ( NM_001305244.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292173.1 Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu73419
Clone ID Related Accession (Same CDS sequence)	NM_001305244.1
Accession Version	NM_001305244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	proteasome inhibitor PI31 subunit isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE649179.1, AK083431.1 and AL928988.14. On Mar 5, 2015 this sequence version replaced XM_006499307.2. Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate start codon, and lacks two exons in the 5' coding region compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK083431.1, BB621704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTTGGG CACGGGCGAC CAGGACCTAT AAGAACAGTG AGGAGCTTCG GTCTCAGATC 
AGATCTGGAA TTATCACACC TATCCATGAG CAGTGGGAGA AGGCTCGTGC AAACAGTCCT 
CCGAGGGAGT TCCCACCTGC CACTGCCAGA GAGGTGGACC CACTCCAGAT TTCCTCACAC 
CGTCCTCATA CTAGCAGGCA GCCTGCTTGG CGTGATCCCC TGAGCCCATT TGCTGTCGGG 
GGAGATGACC TAGACCCTTT TGGGTGTCAG AGAGGTGGCA TGATTGTGGA TCCCCTGAGA 
TCCGGCTTCC CAAGAGTACT TATTGACCCA TCCTCAGGCC TCCCAAACCG GCTTCCTCCT 
GGTGCTGTGC CCCCAGGAGC TCGCTTTGAC CCCTTTGGAC CCATTGGGAC CAGCCCATCT 
GGACCTAACC CTGACCATCT TCCCCCACCG GGCTACGATG ACATGTACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292173.1
CDS	278..751
Translation	MPWARATRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL

Target ORF information:

RefSeq Version	NM_001305244.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305244.1

ORF Insert Sequence:

ATGCCTTGGG CACGGGCGAC CAGGACCTAT AAGAACAGTG AGGAGCTTCG GTCTCAGATC 
AGATCTGGAA TTATCACACC TATCCATGAG CAGTGGGAGA AGGCTCGTGC AAACAGTCCT 
CCGAGGGAGT TCCCACCTGC CACTGCCAGA GAGGTGGACC CACTCCAGAT TTCCTCACAC 
CGTCCTCATA CTAGCAGGCA GCCTGCTTGG CGTGATCCCC TGAGCCCATT TGCTGTCGGG 
GGAGATGACC TAGACCCTTT TGGGTGTCAG AGAGGTGGCA TGATTGTGGA TCCCCTGAGA 
TCCGGCTTCC CAAGAGTACT TATTGACCCA TCCTCAGGCC TCCCAAACCG GCTTCCTCCT 
GGTGCTGTGC CCCCAGGAGC TCGCTTTGAC CCCTTTGGAC CCATTGGGAC CAGCCCATCT 
GGACCTAACC CTGACCATCT TCCCCCACCG GGCTACGATG ACATGTACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psmf1 ( XM_006499306.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499369.1 Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30340
Clone ID Related Accession (Same CDS sequence)	XM_006499306.4 , XM_006499306.3
Accession Version	XM_006499306.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	proteasome inhibitor PI31 subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499306.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC CGCAAACATC CATGGCGGGT CTCGAGGTTC TCTTCGCGTC GGCCGCTCCA 
TCCATGAGCT GCCCGCAGGA CGCACTCGTC TGCTTCCTGC ACTGGGAAGT GGTGACAAAC 
GGCTACTATG CCTTGGGCAC GGGCGACCAG CCAGGTCCCA GTGATAAGAA ATCAGAACTA 
CTGCCAGCTA AGTGGAACAG CAATAAAGAA CTGTATGCCC TCCGGTATGA GTCTAAGGAT 
GGAGCCAGAA AACTCCTTCT GAAAGCTGTC TCTGTGGAGA ACGGCATGAT CATCAACGTG 
CTGGAACTTG GCACACAGCA GGTGGCAGAC TTGACCCTGA ACTTAGATGA TTATATTGAT 
GCAGAAGACC TGAGTGACTT CCACAGGACC TATAAGAACA GTGAGGAGCT TCGGTCTCAG 
ATCAGATCTG GAATTATCAC ACCTATCCAT GAGCAGTGGG AGAAGGCTCG TGCAAACAGT 
CCTCCGAGGG AGTTCCCACC TGCCACTGCC AGAGAGGTGG ACCCACTCCA GATTTCCTCA 
CACCGTCCTC ATACTAGCAG GCAGCCTGCT TGGCGTGATC CCCTGAGCCC ATTTGCTGTC 
GGGGGAGATG ACCTAGACCC TTTTGGGTGT CAGAGAGGTG GCATGATTGT GGATCCCCTG 
AGATCCGGCT TCCCAAGAGT ACTTATTGAC CCATCCTCAG GCCTCCCAAA CCGGCTTCCT 
CCTGGTGCTG TGCCCCCAGG AGCTCGCTTT GACCCCTTTG GACCCATTGG GACCAGCCCA 
TCTGGACCTA ACCCTGACCA TCTTCCCCCA CCGGGCTACG ATGACATGTA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499369.1
CDS	182..1018
Translation	MKKPQTSMAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL

Target ORF information:

RefSeq Version	XM_006499306.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499306.3

ORF Insert Sequence:

ATGAAGAAGC CGCAAACATC CATGGCGGGT CTCGAGGTTC TCTTCGCGTC GGCCGCTCCA 
TCCATGAGCT GCCCGCAGGA CGCACTCGTC TGCTTCCTGC ACTGGGAAGT GGTGACAAAC 
GGCTACTATG CCTTGGGCAC GGGCGACCAG CCAGGTCCCA GTGATAAGAA ATCAGAACTA 
CTGCCAGCTA AGTGGAACAG CAATAAAGAA CTGTATGCCC TCCGGTATGA GTCTAAGGAT 
GGAGCCAGAA AACTCCTTCT GAAAGCTGTC TCTGTGGAGA ACGGCATGAT CATCAACGTG 
CTGGAACTTG GCACACAGCA GGTGGCAGAC TTGACCCTGA ACTTAGATGA TTATATTGAT 
GCAGAAGACC TGAGTGACTT CCACAGGACC TATAAGAACA GTGAGGAGCT TCGGTCTCAG 
ATCAGATCTG GAATTATCAC ACCTATCCAT GAGCAGTGGG AGAAGGCTCG TGCAAACAGT 
CCTCCGAGGG AGTTCCCACC TGCCACTGCC AGAGAGGTGG ACCCACTCCA GATTTCCTCA 
CACCGTCCTC ATACTAGCAG GCAGCCTGCT TGGCGTGATC CCCTGAGCCC ATTTGCTGTC 
GGGGGAGATG ACCTAGACCC TTTTGGGTGT CAGAGAGGTG GCATGATTGT GGATCCCCTG 
AGATCCGGCT TCCCAAGAGT ACTTATTGAC CCATCCTCAG GCCTCCCAAA CCGGCTTCCT 
CCTGGTGCTG TGCCCCCAGG AGCTCGCTTT GACCCCTTTG GACCCATTGG GACCAGCCCA 
TCTGGACCTA ACCCTGACCA TCTTCCCCCA CCGGGCTACG ATGACATGTA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psmf1 ( NM_212446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997611.1 Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14676
Clone ID Related Accession (Same CDS sequence)	NM_212446.2
Accession Version	NM_212446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	proteasome inhibitor PI31 subunit isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE649179.1, AK053924.1 and AK083431.1. On Mar 5, 2015 this sequence version replaced NM_212446.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660817.208633.1, AK031363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTC TCGAGGTTCT CTTCGCGTCG GCCGCTCCAT CCATGAGCTG CCCGCAGGAC 
GCACTCGTCT GCTTCCTGCA CTGGGAAGTG GTGACAAACG GCTACTATGC CTTGGGCACG 
GGCGACCAGC CAGGTCCCAG TGATAAGAAA TCAGAACTAC TGCCAGCTAA GTGGAACAGC 
AATAAAGAAC TGTATGCCCT CCGGTATGAG TCTAAGGATG GAGCCAGAAA ACTCCTTCTG 
AAAGCTGTCT CTGTGGAGAA CGGCATGATC ATCAACGTGC TGGAACTTGG CACACAGCAG 
GTGGCAGACT TGACCCTGAA CTTAGATGAT TATATTGATG CAGAAGACCT GAGTGACTTC 
CACAGGACCT ATAAGAACAG TGAGGAGCTT CGGTCTCAGA TCAGATCTGG AATTATCACA 
CCTATCCATG AGCAGTGGGA GAAGGCTCGT GCAAACAGTC CTCCGAGGGA GTTCCCACCT 
GCCACTGCCA GAGAGGTGGA CCCACTCCAG ATTTCCTCAC ACCGTCCTCA TACTAGCAGG 
CAGCCTGCTT GGCGTGATCC CCTGAGCCCA TTTGCTGTCG GGGGAGATGA CCTAGACCCT 
TTTGGGTGTC AGAGAGGTGG CATGATTGTG GATCCCCTGA GATCCGGCTT CCCAAGAGTA 
CTTATTGACC CATCCTCAGG CCTCCCAAAC CGGCTTCCTC CTGGTGCTGT GCCCCCAGGA 
GCTCGCTTTG ACCCCTTTGG ACCCATTGGG ACCAGCCCAT CTGGACCTAA CCCTGACCAT 
CTTCCCCCAC CGGGCTACGA TGACATGTAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997611.1
CDS	172..987
Translation	MAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL

Target ORF information:

RefSeq Version	NM_212446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212446.2

ORF Insert Sequence:

ATGGCGGGTC TCGAGGTTCT CTTCGCGTCG GCCGCTCCAT CCATGAGCTG CCCGCAGGAC 
GCACTCGTCT GCTTCCTGCA CTGGGAAGTG GTGACAAACG GCTACTATGC CTTGGGCACG 
GGCGACCAGC CAGGTCCCAG TGATAAGAAA TCAGAACTAC TGCCAGCTAA GTGGAACAGC 
AATAAAGAAC TGTATGCCCT CCGGTATGAG TCTAAGGATG GAGCCAGAAA ACTCCTTCTG 
AAAGCTGTCT CTGTGGAGAA CGGCATGATC ATCAACGTGC TGGAACTTGG CACACAGCAG 
GTGGCAGACT TGACCCTGAA CTTAGATGAT TATATTGATG CAGAAGACCT GAGTGACTTC 
CACAGGACCT ATAAGAACAG TGAGGAGCTT CGGTCTCAGA TCAGATCTGG AATTATCACA 
CCTATCCATG AGCAGTGGGA GAAGGCTCGT GCAAACAGTC CTCCGAGGGA GTTCCCACCT 
GCCACTGCCA GAGAGGTGGA CCCACTCCAG ATTTCCTCAC ACCGTCCTCA TACTAGCAGG 
CAGCCTGCTT GGCGTGATCC CCTGAGCCCA TTTGCTGTCG GGGGAGATGA CCTAGACCCT 
TTTGGGTGTC AGAGAGGTGG CATGATTGTG GATCCCCTGA GATCCGGCTT CCCAAGAGTA 
CTTATTGACC CATCCTCAGG CCTCCCAAAC CGGCTTCCTC CTGGTGCTGT GCCCCCAGGA 
GCTCGCTTTG ACCCCTTTGG ACCCATTGGG ACCAGCCCAT CTGGACCTAA CCCTGACCAT 
CTTCCCCCAC CGGGCTACGA TGACATGTAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psmf1 ( XM_006499306.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499369.1 Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30340
Clone ID Related Accession (Same CDS sequence)	XM_006499306.4 , XM_006499306.3
Accession Version	XM_006499306.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteasome inhibitor PI31 subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499306.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC CGCAAACATC CATGGCGGGT CTCGAGGTTC TCTTCGCGTC GGCCGCTCCA 
TCCATGAGCT GCCCGCAGGA CGCACTCGTC TGCTTCCTGC ACTGGGAAGT GGTGACAAAC 
GGCTACTATG CCTTGGGCAC GGGCGACCAG CCAGGTCCCA GTGATAAGAA ATCAGAACTA 
CTGCCAGCTA AGTGGAACAG CAATAAAGAA CTGTATGCCC TCCGGTATGA GTCTAAGGAT 
GGAGCCAGAA AACTCCTTCT GAAAGCTGTC TCTGTGGAGA ACGGCATGAT CATCAACGTG 
CTGGAACTTG GCACACAGCA GGTGGCAGAC TTGACCCTGA ACTTAGATGA TTATATTGAT 
GCAGAAGACC TGAGTGACTT CCACAGGACC TATAAGAACA GTGAGGAGCT TCGGTCTCAG 
ATCAGATCTG GAATTATCAC ACCTATCCAT GAGCAGTGGG AGAAGGCTCG TGCAAACAGT 
CCTCCGAGGG AGTTCCCACC TGCCACTGCC AGAGAGGTGG ACCCACTCCA GATTTCCTCA 
CACCGTCCTC ATACTAGCAG GCAGCCTGCT TGGCGTGATC CCCTGAGCCC ATTTGCTGTC 
GGGGGAGATG ACCTAGACCC TTTTGGGTGT CAGAGAGGTG GCATGATTGT GGATCCCCTG 
AGATCCGGCT TCCCAAGAGT ACTTATTGAC CCATCCTCAG GCCTCCCAAA CCGGCTTCCT 
CCTGGTGCTG TGCCCCCAGG AGCTCGCTTT GACCCCTTTG GACCCATTGG GACCAGCCCA 
TCTGGACCTA ACCCTGACCA TCTTCCCCCA CCGGGCTACG ATGACATGTA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499369.1
CDS	192..1028
Translation	MKKPQTSMAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL

Target ORF information:

RefSeq Version	XM_006499306.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499306.4

ORF Insert Sequence:

ATGAAGAAGC CGCAAACATC CATGGCGGGT CTCGAGGTTC TCTTCGCGTC GGCCGCTCCA 
TCCATGAGCT GCCCGCAGGA CGCACTCGTC TGCTTCCTGC ACTGGGAAGT GGTGACAAAC 
GGCTACTATG CCTTGGGCAC GGGCGACCAG CCAGGTCCCA GTGATAAGAA ATCAGAACTA 
CTGCCAGCTA AGTGGAACAG CAATAAAGAA CTGTATGCCC TCCGGTATGA GTCTAAGGAT 
GGAGCCAGAA AACTCCTTCT GAAAGCTGTC TCTGTGGAGA ACGGCATGAT CATCAACGTG 
CTGGAACTTG GCACACAGCA GGTGGCAGAC TTGACCCTGA ACTTAGATGA TTATATTGAT 
GCAGAAGACC TGAGTGACTT CCACAGGACC TATAAGAACA GTGAGGAGCT TCGGTCTCAG 
ATCAGATCTG GAATTATCAC ACCTATCCAT GAGCAGTGGG AGAAGGCTCG TGCAAACAGT 
CCTCCGAGGG AGTTCCCACC TGCCACTGCC AGAGAGGTGG ACCCACTCCA GATTTCCTCA 
CACCGTCCTC ATACTAGCAG GCAGCCTGCT TGGCGTGATC CCCTGAGCCC ATTTGCTGTC 
GGGGGAGATG ACCTAGACCC TTTTGGGTGT CAGAGAGGTG GCATGATTGT GGATCCCCTG 
AGATCCGGCT TCCCAAGAGT ACTTATTGAC CCATCCTCAG GCCTCCCAAA CCGGCTTCCT 
CCTGGTGCTG TGCCCCCAGG AGCTCGCTTT GACCCCTTTG GACCCATTGG GACCAGCCCA 
TCTGGACCTA ACCCTGACCA TCTTCCCCCA CCGGGCTACG ATGACATGTA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

PI31 is a modulator of proteasome formation and antigen processing.
Proceedings of the National Academy of Sciences of the United States of America99(22)14344-9(2002 Oct)
Zaiss DM,Standera S,Kloetzel PM,Sijts AJ

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Psmf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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