Uba1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba1
Entrez Gene ID	35998
Full Name	Ubiquitin activating enzyme 1
Synonyms	2/31,CG1782,DmUba1,Dmel\CG1782,E1,FBtr0088499,UBA-1,Uba 1,l(2)03405,l(2)05642,l(2)s3484,uba-1,uba1
General protein information	Preferred Names Ubiquitin activating enzyme 1 Names CG1782 gene product from transcript CG1782-RA CG1782-PA CG1782-PC E1 E1 ligase Uba1-PA Uba1-PC ubiquitin activating enzyme ubiquitin activating enzyme 1 ubiquitin-activating enzyme (E1)
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46A1-46A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057962.5	NP_477310.2	ubiquitin activating enzyme 1, isoform A
NM_001273902.1	NP_001260831.1	ubiquitin activating enzyme 1, isoform C

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Uba1, the E1 enzyme used in ubiquitylation, is required for autophagy and reduction of cell size.
Title: Uba1 functions in Atg7- and Atg3-independent autophagy.

These mutants of the E1, the ubiquitin activating enzyme exhibited further reduced lifespan and severe motor impairment.
Title: Mutation in E1, the ubiquitin activating enzyme, reduces Drosophila lifespan and results in motor impairment.

knockdown of SUMO or its E1 and E2 enzymes robustly disrupts proliferating cells in the developing eye, without any detectable effects on the development or differentiation of neighboring post-mitotic cells
Title: A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development.

Data demonstrate that maintaining sufficient E1 function is required both cell autonomously and non-cell autonomously to prevent inappropriate Ras-ERK-dependent growth and proliferation in vivo.
Title: Impairment of ubiquitylation by mutation in Drosophila E1 promotes both cell-autonomous and non-cell-autonomous Ras-ERK activation in vivo.

This study showed that a reduction in uba1 activity can promote tissue growth in a multicellular organism and raise the possibility that changes in uba1 activity may occur during normal development or in cancer.
Title: Mutation of the gene encoding the ubiquitin activating enzyme ubal causes tissue overgrowth in Drosophila.

The following Uba1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09394	NM_001273902.1 Latest version!	Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant C (Uba1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12341	NM_057962.5 Latest version!	Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant A (Uba1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba1 ( NM_001273902.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260831.1 Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant C (Uba1), mRNA.

Price & Availability↑

CloneID	OFa09394
Clone ID Related Accession (Same CDS sequence)	NM_001273902.1
Accession Version	NM_001273902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin activating enzyme 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGAA ACAGTGCCGC CGCCGGCGGA GATATCGATG AATCGCTGTA CTCGCGCCAA 
TTGTACGTCC TGGGTCACGA TGCGATGCGT CGGATGGCCA ATTCGGATAT CCTTCTGTCG 
GGACTTGGAG GACTCGGCCT GGAGATAGCC AAGAATGTGA TTCTGGGCGG CGTGAAATCG 
ATCACCTTGC ATGACACAGC AACTTGTGGG CTCCATGACT TGTCGTCGCA ATTCTATCTC 
ACGGAAGCCG ATATCGGCAA GAATCGTGCG GAGGCCTCTT GCGCCCAGTT GGCCGAGTTA 
AACAACTATG TGCGCACCGT TTCGCACACG GGGCCGCTTA CCGAGGAGTT CCTGCGCAAG 
TTTCGCGTTG TGGTACTGAC CAACTCCGAC GGGGAGGAGC AGCAGAGGAT CGCCAAGTTT 
GCCCATGAAA ATGGCATTGC TCTGATCATC GCCGAGACGC GCGGACTGTT CGCCAAGGTG 
TTCTGTGACT TTGGCGAGAG CTTTACCATT TACGATCAGG ATGGCACACA GCCGATCTCC 
ACCATGATAG CTAGTATCAC GCACGACGCA CAGGGAGTCG TCACCTGCCT GGACGAGACG 
CGCCATGGTT TTAATGACGG TGACTACGTC ACCTTCTCGG AGGTGCAGGG CATGCAGGAA 
CTGAACGGTT GTCAGCCCCT TAAGATCACA GTGCTGGGAC CCTACACATT TAGCATCGGT 
GATACCAGCA AGTTTGGAGA ATATAAGAGC GGAGGAGTGG CCACCCAGGT GAAGATGCCC 
AAGACCATCA GCTTCAAGCC CCTGGCACAG GCCACTGAGG AGCCGGAGTT TTTGATCTCC 
GATTTCGCAA AGCTGGACTC GCCGGCCACG CTGCACGTGG CCTTCAATGC CCTCTCCTGC 
TACCGCAAGG CCCATAATGG AGCCTTACCG CGTCCCTGGA ACGAGGAGGA CGCCAACAGC 
TTCCTCGAGG TAGTCCGAGC CAGCAGCAAC GCGGAAGTCG ACGAAAAACT GGTGCTGCAA 
TTCGCCAAAA TCTGCTCGGG TAATACGTGC CCGCTGGACG CGGCCGTTGG TGGAATTGTG 
GCCCAGGAGG TGCTTAAGGC CTGCAGTGGA AAGTTTACAC CCATCTACCA GTGGTTGTAC 
TTTGACGCCC TGGAGTGTCT GCCCACAGAG GGTGTCGAGG AGGCGGATGC CCAGCCGGTG 
GGCTCGCGCT ATGATTCTCA GATTGCCATA TTTGGCAAGA AGTTCCAGGA GAAGTTGGCT 
GACTCGAAAT GGTTTATTGT CGGCGCCGGA GCCATTGGTT GTGAGCTATT GAAAAACTTT 
GGAATGCTGG GCCTGGGAAC TGGCAATGGC CAGATTTTCG TCACAGACAT GGATCTCATT 
GAGAAGTCGA ATCTGAACCG GCAGTTCCTG TTCCGCCCGC ATGATGTGCA GAAGCCTAAA 
TCAATGACGG CCGCCGACGC TATCAAACGG ATGAATCCGG AAGTGAATGT CACCGCATAC 
GAGTTGCGCG TTGGCGCCGA AACAGAGAAA GTCTTTTCCG AAGATTTCTT TGGAAAGTTG 
GATGGTGTAG CCAATGCGCT GGACAACGTG GATGCTCGCA TATATATGGA TCGCAAATGC 
ATCTTCAACC GCATTCCGCT CGTCGAGACC GGAACTCTGG GAACTTTGGG CAACGTTCAG 
GTGATTGTGC CCTTTGCCAC CGAATCTTAT AGCTCCTCCC AGGATCCCCC AGAAAAGAGT 
ATTCCCATCT GCACGCTGAA GAACTTCCCC AATGCCATCG AGCACACTCT GCAGTGGGCG 
CGTGACGCCT TTGAGGGCGT CTTCAAGCAG TCTGCAGAAA ATGCTGCTCA GTACATCGCC 
GATCCTCAGT TCACCGAGCG AATCGCCAAG CTGCCTGGTA TTCAGCCTCT GGAGATCCTC 
GATTCCATCA AGAAAGCTCT GATCGACGAC AAACCCAAGA GTTTCGCGCA CTGCGTGGAG 
TGGGCCCGTC TTTATTGGGA GGATCAATAT GTTAACCAGA TTAAGCAGTT GCTCTTTAAC 
TTCCCACCTG ATCAGATCAC CTCCAGTGGT CAGCCGTTTT GGTCTGGTCC CAAGCGGTGC 
CCCGATCCCC TGGTCTTTGA TGTCAACGAT CCCATGCACC TCGACTTTAT CTACGCAGCC 
GCCAATCTTC GAGCTGAGGT CTATGGCATT GAGCAGGTTC GCAATCGCGA GACGATCGCA 
GAGCTGGTAC AGAAGGTTAA GGTGCCCGAG TTTAAACCCC GTTCGGGCGT GAAAATAGAA 
ACCAATGAGG CAGCAGCCGC CGCCTCGGCC AATAACTTCG ATGACGGCGA GTTGGACCAG 
GATCGCGTTG ACAAGATCAT TTCAGAGCTG CTAAAGAACG CCGACAAAAG CTCCAAGATC 
ACGCCACTTG AGTTTGAAAA GGACGACGAC AGTAACTTGC ACATGGACTT CATTGTGGCC 
TGCTCAAATC TACGCGCCGC CAACTACAAG ATTCCGCCAG CGGATCGCCA CAAGTCCAAG 
TTGATTGCTG GCAAAATCAT ACCTGCTATT GCCACCACCA CGTCGGTGCT CTCTGGATTG 
GCTGTGCTCG AGGTGATTAA GCTGATCGTC GGCCACCGGG ATCTCGTAAA ATTCAAGAAC 
GGCTTCGCCA ACTTGGCATT GCCGTTCATG GCCTTCTCTG AGCCGCTTCC TGCCGCCAAG 
AACACATACT ACGGCAAGGA GTGGACGCTG TGGGACCGTT TTGAGGTGAC CGGGGAGCTG 
TCGCTGCAGG AATTCCTCAA CTATTTCGAG GAGAATGAGA AGCTTAAGAT CACCATGCTC 
TCGCAGGGCG TGTCCATGCT GTACTCGTTC TTCATGCCCA AGGCCAAGTG CTCTGAGAGA 
CTGCCGTTGC CGATGTCGGA GGTCGTGCGT CGCGTGTCCA AGCGTCGCCT GGAGCCACAC 
GAGCGATCCC TGGTTTTCGA GATCTGCTGC AACGATGTGG ATGGCGAGGA TGTGGAGGTG 
CCCTACGTCC GTTACACGCT GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260831.1
CDS	838..3864
Translation	MAGNSAAAGGDIDESLYSRQLYVLGHDAMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGKNRAEASCAQLAELNNYVRTVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHENGIALIIAETRGLFAKVFCDFGESFTIYDQDGTQPISTMIASITHDAQGVVTCLDETRHGFNDGDYVTFSEVQGMQELNGCQPLKITVLGPYTFSIGDTSKFGEYKSGGVATQVKMPKTISFKPLAQATEEPEFLISDFAKLDSPATLHVAFNALSCYRKAHNGALPRPWNEEDANSFLEVVRASSNAEVDEKLVLQFAKICSGNTCPLDAAVGGIVAQEVLKACSGKFTPIYQWLYFDALECLPTEGVEEADAQPVGSRYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGVANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIVPFATESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDAFEGVFKQSAENAAQYIADPQFTERIAKLPGIQPLEILDSIKKALIDDKPKSFAHCVEWARLYWEDQYVNQIKQLLFNFPPDQITSSGQPFWSGPKRCPDPLVFDVNDPMHLDFIYAAANLRAEVYGIEQVRNRETIAELVQKVKVPEFKPRSGVKIETNEAAAAASANNFDDGELDQDRVDKIISELLKNADKSSKITPLEFEKDDDSNLHMDFIVACSNLRAANYKIPPADRHKSKLIAGKIIPAIATTTSVLSGLAVLEVIKLIVGHRDLVKFKNGFANLALPFMAFSEPLPAAKNTYYGKEWTLWDRFEVTGELSLQEFLNYFEENEKLKITMLSQGVSMLYSFFMPKAKCSERLPLPMSEVVRRVSKRRLEPHERSLVFEICCNDVDGEDVEVPYVRYTLP

Target ORF information:

RefSeq Version	NM_001273902.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant C (Uba1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273902.1

ORF Insert Sequence:

ATGGCTGGAA ACAGTGCCGC CGCCGGCGGA GATATCGATG AATCGCTGTA CTCGCGCCAA 
TTGTACGTCC TGGGTCACGA TGCGATGCGT CGGATGGCCA ATTCGGATAT CCTTCTGTCG 
GGACTTGGAG GACTCGGCCT GGAGATAGCC AAGAATGTGA TTCTGGGCGG CGTGAAATCG 
ATCACCTTGC ATGACACAGC AACTTGTGGG CTCCATGACT TGTCGTCGCA ATTCTATCTC 
ACGGAAGCCG ATATCGGCAA GAATCGTGCG GAGGCCTCTT GCGCCCAGTT GGCCGAGTTA 
AACAACTATG TGCGCACCGT TTCGCACACG GGGCCGCTTA CCGAGGAGTT CCTGCGCAAG 
TTTCGCGTTG TGGTACTGAC CAACTCCGAC GGGGAGGAGC AGCAGAGGAT CGCCAAGTTT 
GCCCATGAAA ATGGCATTGC TCTGATCATC GCCGAGACGC GCGGACTGTT CGCCAAGGTG 
TTCTGTGACT TTGGCGAGAG CTTTACCATT TACGATCAGG ATGGCACACA GCCGATCTCC 
ACCATGATAG CTAGTATCAC GCACGACGCA CAGGGAGTCG TCACCTGCCT GGACGAGACG 
CGCCATGGTT TTAATGACGG TGACTACGTC ACCTTCTCGG AGGTGCAGGG CATGCAGGAA 
CTGAACGGTT GTCAGCCCCT TAAGATCACA GTGCTGGGAC CCTACACATT TAGCATCGGT 
GATACCAGCA AGTTTGGAGA ATATAAGAGC GGAGGAGTGG CCACCCAGGT GAAGATGCCC 
AAGACCATCA GCTTCAAGCC CCTGGCACAG GCCACTGAGG AGCCGGAGTT TTTGATCTCC 
GATTTCGCAA AGCTGGACTC GCCGGCCACG CTGCACGTGG CCTTCAATGC CCTCTCCTGC 
TACCGCAAGG CCCATAATGG AGCCTTACCG CGTCCCTGGA ACGAGGAGGA CGCCAACAGC 
TTCCTCGAGG TAGTCCGAGC CAGCAGCAAC GCGGAAGTCG ACGAAAAACT GGTGCTGCAA 
TTCGCCAAAA TCTGCTCGGG TAATACGTGC CCGCTGGACG CGGCCGTTGG TGGAATTGTG 
GCCCAGGAGG TGCTTAAGGC CTGCAGTGGA AAGTTTACAC CCATCTACCA GTGGTTGTAC 
TTTGACGCCC TGGAGTGTCT GCCCACAGAG GGTGTCGAGG AGGCGGATGC CCAGCCGGTG 
GGCTCGCGCT ATGATTCTCA GATTGCCATA TTTGGCAAGA AGTTCCAGGA GAAGTTGGCT 
GACTCGAAAT GGTTTATTGT CGGCGCCGGA GCCATTGGTT GTGAGCTATT GAAAAACTTT 
GGAATGCTGG GCCTGGGAAC TGGCAATGGC CAGATTTTCG TCACAGACAT GGATCTCATT 
GAGAAGTCGA ATCTGAACCG GCAGTTCCTG TTCCGCCCGC ATGATGTGCA GAAGCCTAAA 
TCAATGACGG CCGCCGACGC TATCAAACGG ATGAATCCGG AAGTGAATGT CACCGCATAC 
GAGTTGCGCG TTGGCGCCGA AACAGAGAAA GTCTTTTCCG AAGATTTCTT TGGAAAGTTG 
GATGGTGTAG CCAATGCGCT GGACAACGTG GATGCTCGCA TATATATGGA TCGCAAATGC 
ATCTTCAACC GCATTCCGCT CGTCGAGACC GGAACTCTGG GAACTTTGGG CAACGTTCAG 
GTGATTGTGC CCTTTGCCAC CGAATCTTAT AGCTCCTCCC AGGATCCCCC AGAAAAGAGT 
ATTCCCATCT GCACGCTGAA GAACTTCCCC AATGCCATCG AGCACACTCT GCAGTGGGCG 
CGTGACGCCT TTGAGGGCGT CTTCAAGCAG TCTGCAGAAA ATGCTGCTCA GTACATCGCC 
GATCCTCAGT TCACCGAGCG AATCGCCAAG CTGCCTGGTA TTCAGCCTCT GGAGATCCTC 
GATTCCATCA AGAAAGCTCT GATCGACGAC AAACCCAAGA GTTTCGCGCA CTGCGTGGAG 
TGGGCCCGTC TTTATTGGGA GGATCAATAT GTTAACCAGA TTAAGCAGTT GCTCTTTAAC 
TTCCCACCTG ATCAGATCAC CTCCAGTGGT CAGCCGTTTT GGTCTGGTCC CAAGCGGTGC 
CCCGATCCCC TGGTCTTTGA TGTCAACGAT CCCATGCACC TCGACTTTAT CTACGCAGCC 
GCCAATCTTC GAGCTGAGGT CTATGGCATT GAGCAGGTTC GCAATCGCGA GACGATCGCA 
GAGCTGGTAC AGAAGGTTAA GGTGCCCGAG TTTAAACCCC GTTCGGGCGT GAAAATAGAA 
ACCAATGAGG CAGCAGCCGC CGCCTCGGCC AATAACTTCG ATGACGGCGA GTTGGACCAG 
GATCGCGTTG ACAAGATCAT TTCAGAGCTG CTAAAGAACG CCGACAAAAG CTCCAAGATC 
ACGCCACTTG AGTTTGAAAA GGACGACGAC AGTAACTTGC ACATGGACTT CATTGTGGCC 
TGCTCAAATC TACGCGCCGC CAACTACAAG ATTCCGCCAG CGGATCGCCA CAAGTCCAAG 
TTGATTGCTG GCAAAATCAT ACCTGCTATT GCCACCACCA CGTCGGTGCT CTCTGGATTG 
GCTGTGCTCG AGGTGATTAA GCTGATCGTC GGCCACCGGG ATCTCGTAAA ATTCAAGAAC 
GGCTTCGCCA ACTTGGCATT GCCGTTCATG GCCTTCTCTG AGCCGCTTCC TGCCGCCAAG 
AACACATACT ACGGCAAGGA GTGGACGCTG TGGGACCGTT TTGAGGTGAC CGGGGAGCTG 
TCGCTGCAGG AATTCCTCAA CTATTTCGAG GAGAATGAGA AGCTTAAGAT CACCATGCTC 
TCGCAGGGCG TGTCCATGCT GTACTCGTTC TTCATGCCCA AGGCCAAGTG CTCTGAGAGA 
CTGCCGTTGC CGATGTCGGA GGTCGTGCGT CGCGTGTCCA AGCGTCGCCT GGAGCCACAC 
GAGCGATCCC TGGTTTTCGA GATCTGCTGC AACGATGTGG ATGGCGAGGA TGTGGAGGTG 
CCCTACGTCC GTTACACGCT GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba1 ( NM_057962.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477310.2 Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant A (Uba1), mRNA.

Price & Availability↑

CloneID	OFa12341
Clone ID Related Accession (Same CDS sequence)	NM_057962.5
Accession Version	NM_057962.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin activating enzyme 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057962.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG GTCAGCAATT GATGGCCGAT TCGCCAGCCT GCAGCTCAAG TGACCATTCG 
CCGCTGGCCA AAAAAAGGCG TTTAGAGTTG TTGGGTGACG GAGTGGGGGC AATGGGAATG 
GAAGAGGAAG AAGCCGGCGG AGCAACGAGC AGCAGTAGCA GCAACAACTA CGAAAATCAC 
GACAAAGCAG GCGGCGTTGA CATTTCGGGC AAGTGCCAGA GCAAGACGAC TAGCGAGCAC 
CAGCTGGAAT TGTGCGTATC GTCGTCTACC TCTGACACAT GCGGCGTTAA TGCTGCGGCG 
AAGCGAGCCG AAAAGCAGAA ACTGAAACAG AGCCAAGGAA GCGGCGACGA CGTTAGTACC 
TCGTCAGCAG TGGAAAAGGA AGGAAGCAAG CGGCCGGAGA AGCGCAATTA CCTGAACACC 
ACCACAATAG AATCGCAAGA AGCCTCCTCC AGTGCATCCG CCACTAGCAA CAGCAACAAC 
AACGTCAGCT CCAGCAACAA TAACAGTAAT AACAGCACTA ATAACCGACA ACTGGAAGGA 
AGCAGCAACA TGGCTGGAAA CAGTGCCGCC GCCGGCGGAG ATATCGATGA ATCGCTGTAC 
TCGCGCCAAT TGTACGTCCT GGGTCACGAT GCGATGCGTC GGATGGCCAA TTCGGATATC 
CTTCTGTCGG GACTTGGAGG ACTCGGCCTG GAGATAGCCA AGAATGTGAT TCTGGGCGGC 
GTGAAATCGA TCACCTTGCA TGACACAGCA ACTTGTGGGC TCCATGACTT GTCGTCGCAA 
TTCTATCTCA CGGAAGCCGA TATCGGCAAG AATCGTGCGG AGGCCTCTTG CGCCCAGTTG 
GCCGAGTTAA ACAACTATGT GCGCACCGTT TCGCACACGG GGCCGCTTAC CGAGGAGTTC 
CTGCGCAAGT TTCGCGTTGT GGTACTGACC AACTCCGACG GGGAGGAGCA GCAGAGGATC 
GCCAAGTTTG CCCATGAAAA TGGCATTGCT CTGATCATCG CCGAGACGCG CGGACTGTTC 
GCCAAGGTGT TCTGTGACTT TGGCGAGAGC TTTACCATTT ACGATCAGGA TGGCACACAG 
CCGATCTCCA CCATGATAGC TAGTATCACG CACGACGCAC AGGGAGTCGT CACCTGCCTG 
GACGAGACGC GCCATGGTTT TAATGACGGT GACTACGTCA CCTTCTCGGA GGTGCAGGGC 
ATGCAGGAAC TGAACGGTTG TCAGCCCCTT AAGATCACAG TGCTGGGACC CTACACATTT 
AGCATCGGTG ATACCAGCAA GTTTGGAGAA TATAAGAGCG GAGGAGTGGC CACCCAGGTG 
AAGATGCCCA AGACCATCAG CTTCAAGCCC CTGGCACAGG CCACTGAGGA GCCGGAGTTT 
TTGATCTCCG ATTTCGCAAA GCTGGACTCG CCGGCCACGC TGCACGTGGC CTTCAATGCC 
CTCTCCTGCT ACCGCAAGGC CCATAATGGA GCCTTACCGC GTCCCTGGAA CGAGGAGGAC 
GCCAACAGCT TCCTCGAGGT AGTCCGAGCC AGCAGCAACG CGGAAGTCGA CGAAAAACTG 
GTGCTGCAAT TCGCCAAAAT CTGCTCGGGT AATACGTGCC CGCTGGACGC GGCCGTTGGT 
GGAATTGTGG CCCAGGAGGT GCTTAAGGCC TGCAGTGGAA AGTTTACACC CATCTACCAG 
TGGTTGTACT TTGACGCCCT GGAGTGTCTG CCCACAGAGG GTGTCGAGGA GGCGGATGCC 
CAGCCGGTGG GCTCGCGCTA TGATTCTCAG ATTGCCATAT TTGGCAAGAA GTTCCAGGAG 
AAGTTGGCTG ACTCGAAATG GTTTATTGTC GGCGCCGGAG CCATTGGTTG TGAGCTATTG 
AAAAACTTTG GAATGCTGGG CCTGGGAACT GGCAATGGCC AGATTTTCGT CACAGACATG 
GATCTCATTG AGAAGTCGAA TCTGAACCGG CAGTTCCTGT TCCGCCCGCA TGATGTGCAG 
AAGCCTAAAT CAATGACGGC CGCCGACGCT ATCAAACGGA TGAATCCGGA AGTGAATGTC 
ACCGCATACG AGTTGCGCGT TGGCGCCGAA ACAGAGAAAG TCTTTTCCGA AGATTTCTTT 
GGAAAGTTGG ATGGTGTAGC CAATGCGCTG GACAACGTGG ATGCTCGCAT ATATATGGAT 
CGCAAATGCA TCTTCAACCG CATTCCGCTC GTCGAGACCG GAACTCTGGG AACTTTGGGC 
AACGTTCAGG TGATTGTGCC CTTTGCCACC GAATCTTATA GCTCCTCCCA GGATCCCCCA 
GAAAAGAGTA TTCCCATCTG CACGCTGAAG AACTTCCCCA ATGCCATCGA GCACACTCTG 
CAGTGGGCGC GTGACGCCTT TGAGGGCGTC TTCAAGCAGT CTGCAGAAAA TGCTGCTCAG 
TACATCGCCG ATCCTCAGTT CACCGAGCGA ATCGCCAAGC TGCCTGGTAT TCAGCCTCTG 
GAGATCCTCG ATTCCATCAA GAAAGCTCTG ATCGACGACA AACCCAAGAG TTTCGCGCAC 
TGCGTGGAGT GGGCCCGTCT TTATTGGGAG GATCAATATG TTAACCAGAT TAAGCAGTTG 
CTCTTTAACT TCCCACCTGA TCAGATCACC TCCAGTGGTC AGCCGTTTTG GTCTGGTCCC 
AAGCGGTGCC CCGATCCCCT GGTCTTTGAT GTCAACGATC CCATGCACCT CGACTTTATC 
TACGCAGCCG CCAATCTTCG AGCTGAGGTC TATGGCATTG AGCAGGTTCG CAATCGCGAG 
ACGATCGCAG AGCTGGTACA GAAGGTTAAG GTGCCCGAGT TTAAACCCCG TTCGGGCGTG 
AAAATAGAAA CCAATGAGGC AGCAGCCGCC GCCTCGGCCA ATAACTTCGA TGACGGCGAG 
TTGGACCAGG ATCGCGTTGA CAAGATCATT TCAGAGCTGC TAAAGAACGC CGACAAAAGC 
TCCAAGATCA CGCCACTTGA GTTTGAAAAG GACGACGACA GTAACTTGCA CATGGACTTC 
ATTGTGGCCT GCTCAAATCT ACGCGCCGCC AACTACAAGA TTCCGCCAGC GGATCGCCAC 
AAGTCCAAGT TGATTGCTGG CAAAATCATA CCTGCTATTG CCACCACCAC GTCGGTGCTC 
TCTGGATTGG CTGTGCTCGA GGTGATTAAG CTGATCGTCG GCCACCGGGA TCTCGTAAAA 
TTCAAGAACG GCTTCGCCAA CTTGGCATTG CCGTTCATGG CCTTCTCTGA GCCGCTTCCT 
GCCGCCAAGA ACACATACTA CGGCAAGGAG TGGACGCTGT GGGACCGTTT TGAGGTGACC 
GGGGAGCTGT CGCTGCAGGA ATTCCTCAAC TATTTCGAGG AGAATGAGAA GCTTAAGATC 
ACCATGCTCT CGCAGGGCGT GTCCATGCTG TACTCGTTCT TCATGCCCAA GGCCAAGTGC 
TCTGAGAGAC TGCCGTTGCC GATGTCGGAG GTCGTGCGTC GCGTGTCCAA GCGTCGCCTG 
GAGCCACACG AGCGATCCCT GGTTTTCGAG ATCTGCTGCA ACGATGTGGA TGGCGAGGAT 
GTGGAGGTGC CCTACGTCCG TTACACGCTG CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477310.2
CDS	289..3864
Translation	MSSGQQLMADSPACSSSDHSPLAKKRRLELLGDGVGAMGMEEEEAGGATSSSSSNNYENHDKAGGVDISGKCQSKTTSEHQLELCVSSSTSDTCGVNAAAKRAEKQKLKQSQGSGDDVSTSSAVEKEGSKRPEKRNYLNTTTIESQEASSSASATSNSNNNVSSSNNNSNNSTNNRQLEGSSNMAGNSAAAGGDIDESLYSRQLYVLGHDAMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGKNRAEASCAQLAELNNYVRTVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHENGIALIIAETRGLFAKVFCDFGESFTIYDQDGTQPISTMIASITHDAQGVVTCLDETRHGFNDGDYVTFSEVQGMQELNGCQPLKITVLGPYTFSIGDTSKFGEYKSGGVATQVKMPKTISFKPLAQATEEPEFLISDFAKLDSPATLHVAFNALSCYRKAHNGALPRPWNEEDANSFLEVVRASSNAEVDEKLVLQFAKICSGNTCPLDAAVGGIVAQEVLKACSGKFTPIYQWLYFDALECLPTEGVEEADAQPVGSRYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGVANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIVPFATESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDAFEGVFKQSAENAAQYIADPQFTERIAKLPGIQPLEILDSIKKALIDDKPKSFAHCVEWARLYWEDQYVNQIKQLLFNFPPDQITSSGQPFWSGPKRCPDPLVFDVNDPMHLDFIYAAANLRAEVYGIEQVRNRETIAELVQKVKVPEFKPRSGVKIETNEAAAAASANNFDDGELDQDRVDKIISELLKNADKSSKITPLEFEKDDDSNLHMDFIVACSNLRAANYKIPPADRHKSKLIAGKIIPAIATTTSVLSGLAVLEVIKLIVGHRDLVKFKNGFANLALPFMAFSEPLPAAKNTYYGKEWTLWDRFEVTGELSLQEFLNYFEENEKLKITMLSQGVSMLYSFFMPKAKCSERLPLPMSEVVRRVSKRRLEPHERSLVFEICCNDVDGEDVEVPYVRYTLP

Target ORF information:

RefSeq Version	NM_057962.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin activating enzyme 1, transcript variant A (Uba1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057962.5

ORF Insert Sequence:

ATGTCGAGCG GTCAGCAATT GATGGCCGAT TCGCCAGCCT GCAGCTCAAG TGACCATTCG 
CCGCTGGCCA AAAAAAGGCG TTTAGAGTTG TTGGGTGACG GAGTGGGGGC AATGGGAATG 
GAAGAGGAAG AAGCCGGCGG AGCAACGAGC AGCAGTAGCA GCAACAACTA CGAAAATCAC 
GACAAAGCAG GCGGCGTTGA CATTTCGGGC AAGTGCCAGA GCAAGACGAC TAGCGAGCAC 
CAGCTGGAAT TGTGCGTATC GTCGTCTACC TCTGACACAT GCGGCGTTAA TGCTGCGGCG 
AAGCGAGCCG AAAAGCAGAA ACTGAAACAG AGCCAAGGAA GCGGCGACGA CGTTAGTACC 
TCGTCAGCAG TGGAAAAGGA AGGAAGCAAG CGGCCGGAGA AGCGCAATTA CCTGAACACC 
ACCACAATAG AATCGCAAGA AGCCTCCTCC AGTGCATCCG CCACTAGCAA CAGCAACAAC 
AACGTCAGCT CCAGCAACAA TAACAGTAAT AACAGCACTA ATAACCGACA ACTGGAAGGA 
AGCAGCAACA TGGCTGGAAA CAGTGCCGCC GCCGGCGGAG ATATCGATGA ATCGCTGTAC 
TCGCGCCAAT TGTACGTCCT GGGTCACGAT GCGATGCGTC GGATGGCCAA TTCGGATATC 
CTTCTGTCGG GACTTGGAGG ACTCGGCCTG GAGATAGCCA AGAATGTGAT TCTGGGCGGC 
GTGAAATCGA TCACCTTGCA TGACACAGCA ACTTGTGGGC TCCATGACTT GTCGTCGCAA 
TTCTATCTCA CGGAAGCCGA TATCGGCAAG AATCGTGCGG AGGCCTCTTG CGCCCAGTTG 
GCCGAGTTAA ACAACTATGT GCGCACCGTT TCGCACACGG GGCCGCTTAC CGAGGAGTTC 
CTGCGCAAGT TTCGCGTTGT GGTACTGACC AACTCCGACG GGGAGGAGCA GCAGAGGATC 
GCCAAGTTTG CCCATGAAAA TGGCATTGCT CTGATCATCG CCGAGACGCG CGGACTGTTC 
GCCAAGGTGT TCTGTGACTT TGGCGAGAGC TTTACCATTT ACGATCAGGA TGGCACACAG 
CCGATCTCCA CCATGATAGC TAGTATCACG CACGACGCAC AGGGAGTCGT CACCTGCCTG 
GACGAGACGC GCCATGGTTT TAATGACGGT GACTACGTCA CCTTCTCGGA GGTGCAGGGC 
ATGCAGGAAC TGAACGGTTG TCAGCCCCTT AAGATCACAG TGCTGGGACC CTACACATTT 
AGCATCGGTG ATACCAGCAA GTTTGGAGAA TATAAGAGCG GAGGAGTGGC CACCCAGGTG 
AAGATGCCCA AGACCATCAG CTTCAAGCCC CTGGCACAGG CCACTGAGGA GCCGGAGTTT 
TTGATCTCCG ATTTCGCAAA GCTGGACTCG CCGGCCACGC TGCACGTGGC CTTCAATGCC 
CTCTCCTGCT ACCGCAAGGC CCATAATGGA GCCTTACCGC GTCCCTGGAA CGAGGAGGAC 
GCCAACAGCT TCCTCGAGGT AGTCCGAGCC AGCAGCAACG CGGAAGTCGA CGAAAAACTG 
GTGCTGCAAT TCGCCAAAAT CTGCTCGGGT AATACGTGCC CGCTGGACGC GGCCGTTGGT 
GGAATTGTGG CCCAGGAGGT GCTTAAGGCC TGCAGTGGAA AGTTTACACC CATCTACCAG 
TGGTTGTACT TTGACGCCCT GGAGTGTCTG CCCACAGAGG GTGTCGAGGA GGCGGATGCC 
CAGCCGGTGG GCTCGCGCTA TGATTCTCAG ATTGCCATAT TTGGCAAGAA GTTCCAGGAG 
AAGTTGGCTG ACTCGAAATG GTTTATTGTC GGCGCCGGAG CCATTGGTTG TGAGCTATTG 
AAAAACTTTG GAATGCTGGG CCTGGGAACT GGCAATGGCC AGATTTTCGT CACAGACATG 
GATCTCATTG AGAAGTCGAA TCTGAACCGG CAGTTCCTGT TCCGCCCGCA TGATGTGCAG 
AAGCCTAAAT CAATGACGGC CGCCGACGCT ATCAAACGGA TGAATCCGGA AGTGAATGTC 
ACCGCATACG AGTTGCGCGT TGGCGCCGAA ACAGAGAAAG TCTTTTCCGA AGATTTCTTT 
GGAAAGTTGG ATGGTGTAGC CAATGCGCTG GACAACGTGG ATGCTCGCAT ATATATGGAT 
CGCAAATGCA TCTTCAACCG CATTCCGCTC GTCGAGACCG GAACTCTGGG AACTTTGGGC 
AACGTTCAGG TGATTGTGCC CTTTGCCACC GAATCTTATA GCTCCTCCCA GGATCCCCCA 
GAAAAGAGTA TTCCCATCTG CACGCTGAAG AACTTCCCCA ATGCCATCGA GCACACTCTG 
CAGTGGGCGC GTGACGCCTT TGAGGGCGTC TTCAAGCAGT CTGCAGAAAA TGCTGCTCAG 
TACATCGCCG ATCCTCAGTT CACCGAGCGA ATCGCCAAGC TGCCTGGTAT TCAGCCTCTG 
GAGATCCTCG ATTCCATCAA GAAAGCTCTG ATCGACGACA AACCCAAGAG TTTCGCGCAC 
TGCGTGGAGT GGGCCCGTCT TTATTGGGAG GATCAATATG TTAACCAGAT TAAGCAGTTG 
CTCTTTAACT TCCCACCTGA TCAGATCACC TCCAGTGGTC AGCCGTTTTG GTCTGGTCCC 
AAGCGGTGCC CCGATCCCCT GGTCTTTGAT GTCAACGATC CCATGCACCT CGACTTTATC 
TACGCAGCCG CCAATCTTCG AGCTGAGGTC TATGGCATTG AGCAGGTTCG CAATCGCGAG 
ACGATCGCAG AGCTGGTACA GAAGGTTAAG GTGCCCGAGT TTAAACCCCG TTCGGGCGTG 
AAAATAGAAA CCAATGAGGC AGCAGCCGCC GCCTCGGCCA ATAACTTCGA TGACGGCGAG 
TTGGACCAGG ATCGCGTTGA CAAGATCATT TCAGAGCTGC TAAAGAACGC CGACAAAAGC 
TCCAAGATCA CGCCACTTGA GTTTGAAAAG GACGACGACA GTAACTTGCA CATGGACTTC 
ATTGTGGCCT GCTCAAATCT ACGCGCCGCC AACTACAAGA TTCCGCCAGC GGATCGCCAC 
AAGTCCAAGT TGATTGCTGG CAAAATCATA CCTGCTATTG CCACCACCAC GTCGGTGCTC 
TCTGGATTGG CTGTGCTCGA GGTGATTAAG CTGATCGTCG GCCACCGGGA TCTCGTAAAA 
TTCAAGAACG GCTTCGCCAA CTTGGCATTG CCGTTCATGG CCTTCTCTGA GCCGCTTCCT 
GCCGCCAAGA ACACATACTA CGGCAAGGAG TGGACGCTGT GGGACCGTTT TGAGGTGACC 
GGGGAGCTGT CGCTGCAGGA ATTCCTCAAC TATTTCGAGG AGAATGAGAA GCTTAAGATC 
ACCATGCTCT CGCAGGGCGT GTCCATGCTG TACTCGTTCT TCATGCCCAA GGCCAAGTGC 
TCTGAGAGAC TGCCGTTGCC GATGTCGGAG GTCGTGCGTC GCGTGTCCAA GCGTCGCCTG 
GAGCCACACG AGCGATCCCT GGTTTTCGAG ATCTGCTGCA ACGATGTGGA TGGCGAGGAT 
GTGGAGGTGC CCTACGTCCG TTACACGCTG CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Uba1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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