uba-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	uba-1
Entrez Gene ID	177855
Full Name	UBA (human ubiquitin) related
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001268520.2	NP_001255449.1	UBA_e1_C domain-containing protein
NM_001038316.3	NP_001033405.1	UBA (human ubiquitin) related
NM_001038315.5	NP_001033404.1	UBA (human ubiquitin) related
NM_001268520.1	NP_001255449.1	UBA (human ubiquitin) related
NM_001038316.4	NP_001033405.1	UBA_e1_C domain-containing protein
NM_001038315.6	NP_001033404.1	UBA_e1_C domain-containing protein

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

uba-1 plays multiple roles throughout C. elegans development.
Title: E1 ubiquitin-activating enzyme UBA-1 plays multiple roles throughout C. elegans development.

The following uba-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uba-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342933	NM_001038316.4 Latest version!	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa343554	NM_001268520.2 Latest version!	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa344502	NM_001038315.6 Latest version!	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa343554	NM_001268520.1	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa342933	NM_001038316.3	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa344502	NM_001038315.5	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uba-1 ( NM_001038316.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001033405.1 Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

Price & Availability↑

CloneID	OCa342933
Clone ID Related Accession (Same CDS sequence)	NM_001038316.3 , NM_001038316.4
Accession Version	NM_001038316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	UBA (human ubiquitin) related
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001038316.2. COMPLETENESS: incomplete on both ends.

ATGGACACTA GCAATAATGC CGGAGGAGTT GGTGGGAACT CAGATGAGCT TCTCGACAAA 
AATCTTTACT CCCGTCAAAT TTACACGCTC GGCGAATCCG CCATGGTCAA CCTCCGAACT 
GCTTCCGTTC TCATTTCTGG TCTCGGTTCT GTAGGAGTCG AAATTGCAAA GAATCTGATT 
CTTGGAGGAG TTCGGCATGT TACTATTCAT GACACAAAGC TTGCAAAATG GTCTGATCTC 
TCGGCTCAAT ACTACTTGCG CGATGCAGAT GTTGGTCACA ATCGTGCTAC TTCTTGCTAC 
GAGCGTCTTG CAGAGCTTAA TGACAGTGTT AATGTACAAG TATCAACTGA TGAGCTCACT 
GAGGAGTTTG TCAAAACATT TGATCTTGTT GTTCTCACGG ACGCTGCCCG TACTGCTCAA 
CGCCAAATCG CTGCATGGAC ACGAGCCCAC AATAGACGTA TTCTGATTAC TGATGCTCGT 
GGTGTATTCT CATACATCTT CAACGATTTT GGAGACAATT TCCGAATTGA TGACGCTACT 
GGGGAGCAAG TTCGCGAGTT CTTCATTGAG CACATTGATA AAACCACTGG TGAAGTGACA 
ACATTGGAGA ATCTTTTCCA TGGTCTTGAG GATGGAGATC ACGTTACTTT TTCTGAAGTT 
AAAGGACTTA CTGAAATTAA TGGATGTGAG CCACTCAAAA TCACCGTTAA AAATGCATCG 
AAATTCAATA TTGGTGATTT CGCAGTTTCA TTCTCCGACT ACAAAGAAGG TGGAAGATGT 
AGGCAAGTTA AAGTCCCAAC ATCGGTATCT CATGTTCCAT TCGAGAAATC TCTTGTTGAA 
CCAGAATTCG GAATATGGGA CTATGCCAAG TTCGAATACC CATCCCAGCT TCATGCCTTG 
TGGACTGCTC TTTACGCTTT CGAAGAAAAG TATGGAAGAT CCCCTGCTCC TCGATCCACT 
CAGGATGCCG CATTGTTAAA AGAATTGATT CCTTCTGGTA CTGAAGAAAT CCCAGAAAAG 
TTGATCGAAT TGTTCTCGTT TTCTGCATCC GGAAACCTCG TCACTGTGTC ATCTGTTGTT 
GGTGGTATTG CTGCACAAGA AGCAATGAAA GGAGTCACAC ACCATATGAC ACCACTGAAA 
CAATGGCTTC ATTTGGATCA CGTTGAAGTC TTGCCAGGGG ATTGGACTTC TTTCGACAAT 
TCCAAACTTT CGGAAACTGA TTGTCAGCCG AGACAATCTC GTTATGATGG ACAAGCTGCC 
GTATTTGGAT GGCCATATCA AGAGTGTCTA TTCCGTCAAA GATGGTTTGT TGTTGGTGCT 
GGAGCTATCG GATGCGAATT GCTCAAAAAT TTATCAATGA TGGGTGTCGC TTGTGGTGAA 
GGAGGTCTTA TCAAAATTAC TGATATGGAC CAAATTGAAA TTTCGAATCT CAACAGACAA 
TTCCTGTTCA GACGCCGTGA CGTTGGGGGA AAGAAGTCAG AATGTGCCGC CCGTGCTGTG 
ACTGCTTTCA ACTCGGATGT TCGAATTGAA GCTTTGGCAG AACGTGTTGG ATTGGAAACC 
GAGCACATTT TCAATGACGA ATTCTTCGGA GAGCTCAATG GAGTTGCCAA TGCGCTTGAT 
AATGTTGATG CTCGGCGATA CATGGATCGT CGATGCGTCT ACTATCGCTT GCCTTTGCTG 
GAGTCTGGAA CCATGGGAAC CAAAGGAAAC ACTCAAGTTG TTTATCCATA TCTCACGGAG 
TCATACTCGT CGTCTGTCGA TCCACCAGAG AAGGAGATTC CTGTTTGCAC GTTGAAGAAC 
TTCCCCAATG AAATTCAGCA CACAATTCAA TGGGCGCGTG AGCAATTTGA AACATTCTTC 
GCCCAGCCAG GAGAAATGGC CAACAAGTTC CTTTCCGATG AAAGAGGGTT CAATGAGCAT 
GTCGACAAAT TAATTTCTGG ACAACAAATT GATATTCTTC AAAAAGTAAA AGACGCTTTG 
ATTGATGCTC GTCCATCGTC TGCTGAAGAT TGTATTCGTT GGGCTCGCAA CCAATTCCAG 
GAACTCTACC ACAACAATAT TGCTCAGATG CTTCACTCTT TCCCACCAGA TCAACTCACT 
GATTCTGGAG CCAAGTTCTG GTCAGGAGCT AAGCGTTGCC CGCATGTTCT CAATTTTGAT 
CCATCCAAAG AAGAACACTT CAATTTCGTT TTTGCTGCGT CAATTCTCAT TGCCGAGTTA 
TACGGAGTTC AACCAATTTT GGACAGAGAA GAAGTTATTC GTGTCGCTCT TTCGGTGAAT 
CCGGAACCTT TCGAGCCTAA ATCCGGTGTC AAGATCGCTG TAACTGACGC CGAGGCTAAA 
GAACAGAATG AACGTGGAGC TTCTAGCATG ATCGTTGATG ATGATGCTGC CATCGAAGCT 
TTGAAATTGA AATTGGCAAC GCTGAACGTC AAGTCTACAA GCAAACTGAA CTGTGTTGAT 
TTTGAGAAGG ATGATGATTC TAACCATCAT ATGGAGTTCA TCACGGCTGC ATCGAATCTC 
CGAGCCGAAA ACTATGATAT TCTTCCAGCT GATAGAATGA GAACCAAACA AATTGCTGGA 
AAAATTATTC CAGCCATCGC TACCACCACC GCAGCAGTTG CCGGTCTTGT TTGCATCGAA 
CTGTACAAGG TTGTTGACGC AAACGGTATT CCTAAGACAC CAATGGAGCG TTTCAAGAAC 
ACTTTCCTAA ATCTGTCAAT GCCATTCTTC TCCTCTGCCG AACCAATTGG CGCTCCAAAG 
AAAACTTACA TGGACCGAGA GTTTACTCTC TGGGATAGAA TTGACGTTCA AGGACCGCTC 
ACACTTCAAG AATTCATCGA CAATGTACAA AACCAAACTG GTGGATGCGA GGTGTCGATG 
TTATCCGCTG GAGCGTGTCT TTTGTTCTCG TTTTTCATGA ATGCTGGCAA AAAACAAGAA 
AGACTCAAAA CAGAAGTGAA AGCGGTTTAC GAGGAATTGC TGAAGAAATC ACTTCATCCT 
TCTGTTCACG CTTTGGTTCT CGAGCCAATG ATGTCCGATC CAGACGGTGA AGATGTCGAA 
GTTCCATACA TTCGCTACTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033405.1
CDS	1..3087
Translation	MDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001038316.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038316.3

ORF Insert Sequence:

ATGGACACTA GCAATAATGC CGGAGGAGTT GGTGGGAACT CAGATGAGCT TCTCGACAAA 
AATCTTTACT CCCGTCAAAT TTACACGCTC GGCGAATCCG CCATGGTCAA CCTCCGAACT 
GCTTCCGTTC TCATTTCTGG TCTCGGTTCT GTAGGAGTCG AAATTGCAAA GAATCTGATT 
CTTGGAGGAG TTCGGCATGT TACTATTCAT GACACAAAGC TTGCAAAATG GTCTGATCTC 
TCGGCTCAAT ACTACTTGCG CGATGCAGAT GTTGGTCACA ATCGTGCTAC TTCTTGCTAC 
GAGCGTCTTG CAGAGCTTAA TGACAGTGTT AATGTACAAG TATCAACTGA TGAGCTCACT 
GAGGAGTTTG TCAAAACATT TGATCTTGTT GTTCTCACGG ACGCTGCCCG TACTGCTCAA 
CGCCAAATCG CTGCATGGAC ACGAGCCCAC AATAGACGTA TTCTGATTAC TGATGCTCGT 
GGTGTATTCT CATACATCTT CAACGATTTT GGAGACAATT TCCGAATTGA TGACGCTACT 
GGGGAGCAAG TTCGCGAGTT CTTCATTGAG CACATTGATA AAACCACTGG TGAAGTGACA 
ACATTGGAGA ATCTTTTCCA TGGTCTTGAG GATGGAGATC ACGTTACTTT TTCTGAAGTT 
AAAGGACTTA CTGAAATTAA TGGATGTGAG CCACTCAAAA TCACCGTTAA AAATGCATCG 
AAATTCAATA TTGGTGATTT CGCAGTTTCA TTCTCCGACT ACAAAGAAGG TGGAAGATGT 
AGGCAAGTTA AAGTCCCAAC ATCGGTATCT CATGTTCCAT TCGAGAAATC TCTTGTTGAA 
CCAGAATTCG GAATATGGGA CTATGCCAAG TTCGAATACC CATCCCAGCT TCATGCCTTG 
TGGACTGCTC TTTACGCTTT CGAAGAAAAG TATGGAAGAT CCCCTGCTCC TCGATCCACT 
CAGGATGCCG CATTGTTAAA AGAATTGATT CCTTCTGGTA CTGAAGAAAT CCCAGAAAAG 
TTGATCGAAT TGTTCTCGTT TTCTGCATCC GGAAACCTCG TCACTGTGTC ATCTGTTGTT 
GGTGGTATTG CTGCACAAGA AGCAATGAAA GGAGTCACAC ACCATATGAC ACCACTGAAA 
CAATGGCTTC ATTTGGATCA CGTTGAAGTC TTGCCAGGGG ATTGGACTTC TTTCGACAAT 
TCCAAACTTT CGGAAACTGA TTGTCAGCCG AGACAATCTC GTTATGATGG ACAAGCTGCC 
GTATTTGGAT GGCCATATCA AGAGTGTCTA TTCCGTCAAA GATGGTTTGT TGTTGGTGCT 
GGAGCTATCG GATGCGAATT GCTCAAAAAT TTATCAATGA TGGGTGTCGC TTGTGGTGAA 
GGAGGTCTTA TCAAAATTAC TGATATGGAC CAAATTGAAA TTTCGAATCT CAACAGACAA 
TTCCTGTTCA GACGCCGTGA CGTTGGGGGA AAGAAGTCAG AATGTGCCGC CCGTGCTGTG 
ACTGCTTTCA ACTCGGATGT TCGAATTGAA GCTTTGGCAG AACGTGTTGG ATTGGAAACC 
GAGCACATTT TCAATGACGA ATTCTTCGGA GAGCTCAATG GAGTTGCCAA TGCGCTTGAT 
AATGTTGATG CTCGGCGATA CATGGATCGT CGATGCGTCT ACTATCGCTT GCCTTTGCTG 
GAGTCTGGAA CCATGGGAAC CAAAGGAAAC ACTCAAGTTG TTTATCCATA TCTCACGGAG 
TCATACTCGT CGTCTGTCGA TCCACCAGAG AAGGAGATTC CTGTTTGCAC GTTGAAGAAC 
TTCCCCAATG AAATTCAGCA CACAATTCAA TGGGCGCGTG AGCAATTTGA AACATTCTTC 
GCCCAGCCAG GAGAAATGGC CAACAAGTTC CTTTCCGATG AAAGAGGGTT CAATGAGCAT 
GTCGACAAAT TAATTTCTGG ACAACAAATT GATATTCTTC AAAAAGTAAA AGACGCTTTG 
ATTGATGCTC GTCCATCGTC TGCTGAAGAT TGTATTCGTT GGGCTCGCAA CCAATTCCAG 
GAACTCTACC ACAACAATAT TGCTCAGATG CTTCACTCTT TCCCACCAGA TCAACTCACT 
GATTCTGGAG CCAAGTTCTG GTCAGGAGCT AAGCGTTGCC CGCATGTTCT CAATTTTGAT 
CCATCCAAAG AAGAACACTT CAATTTCGTT TTTGCTGCGT CAATTCTCAT TGCCGAGTTA 
TACGGAGTTC AACCAATTTT GGACAGAGAA GAAGTTATTC GTGTCGCTCT TTCGGTGAAT 
CCGGAACCTT TCGAGCCTAA ATCCGGTGTC AAGATCGCTG TAACTGACGC CGAGGCTAAA 
GAACAGAATG AACGTGGAGC TTCTAGCATG ATCGTTGATG ATGATGCTGC CATCGAAGCT 
TTGAAATTGA AATTGGCAAC GCTGAACGTC AAGTCTACAA GCAAACTGAA CTGTGTTGAT 
TTTGAGAAGG ATGATGATTC TAACCATCAT ATGGAGTTCA TCACGGCTGC ATCGAATCTC 
CGAGCCGAAA ACTATGATAT TCTTCCAGCT GATAGAATGA GAACCAAACA AATTGCTGGA 
AAAATTATTC CAGCCATCGC TACCACCACC GCAGCAGTTG CCGGTCTTGT TTGCATCGAA 
CTGTACAAGG TTGTTGACGC AAACGGTATT CCTAAGACAC CAATGGAGCG TTTCAAGAAC 
ACTTTCCTAA ATCTGTCAAT GCCATTCTTC TCCTCTGCCG AACCAATTGG CGCTCCAAAG 
AAAACTTACA TGGACCGAGA GTTTACTCTC TGGGATAGAA TTGACGTTCA AGGACCGCTC 
ACACTTCAAG AATTCATCGA CAATGTACAA AACCAAACTG GTGGATGCGA GGTGTCGATG 
TTATCCGCTG GAGCGTGTCT TTTGTTCTCG TTTTTCATGA ATGCTGGCAA AAAACAAGAA 
AGACTCAAAA CAGAAGTGAA AGCGGTTTAC GAGGAATTGC TGAAGAAATC ACTTCATCCT 
TCTGTTCACG CTTTGGTTCT CGAGCCAATG ATGTCCGATC CAGACGGTGA AGATGTCGAA 
GTTCCATACA TTCGCTACTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba-1 ( NM_001038316.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001033405.1 Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

Price & Availability↑

CloneID	OCa342933
Clone ID Related Accession (Same CDS sequence)	NM_001038316.3 , NM_001038316.4
Accession Version	NM_001038316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	UBA_e1_C domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001038316.3.

ATGGACACTA GCAATAATGC CGGAGGAGTT GGTGGGAACT CAGATGAGCT TCTCGACAAA 
AATCTTTACT CCCGTCAAAT TTACACGCTC GGCGAATCCG CCATGGTCAA CCTCCGAACT 
GCTTCCGTTC TCATTTCTGG TCTCGGTTCT GTAGGAGTCG AAATTGCAAA GAATCTGATT 
CTTGGAGGAG TTCGGCATGT TACTATTCAT GACACAAAGC TTGCAAAATG GTCTGATCTC 
TCGGCTCAAT ACTACTTGCG CGATGCAGAT GTTGGTCACA ATCGTGCTAC TTCTTGCTAC 
GAGCGTCTTG CAGAGCTTAA TGACAGTGTT AATGTACAAG TATCAACTGA TGAGCTCACT 
GAGGAGTTTG TCAAAACATT TGATCTTGTT GTTCTCACGG ACGCTGCCCG TACTGCTCAA 
CGCCAAATCG CTGCATGGAC ACGAGCCCAC AATAGACGTA TTCTGATTAC TGATGCTCGT 
GGTGTATTCT CATACATCTT CAACGATTTT GGAGACAATT TCCGAATTGA TGACGCTACT 
GGGGAGCAAG TTCGCGAGTT CTTCATTGAG CACATTGATA AAACCACTGG TGAAGTGACA 
ACATTGGAGA ATCTTTTCCA TGGTCTTGAG GATGGAGATC ACGTTACTTT TTCTGAAGTT 
AAAGGACTTA CTGAAATTAA TGGATGTGAG CCACTCAAAA TCACCGTTAA AAATGCATCG 
AAATTCAATA TTGGTGATTT CGCAGTTTCA TTCTCCGACT ACAAAGAAGG TGGAAGATGT 
AGGCAAGTTA AAGTCCCAAC ATCGGTATCT CATGTTCCAT TCGAGAAATC TCTTGTTGAA 
CCAGAATTCG GAATATGGGA CTATGCCAAG TTCGAATACC CATCCCAGCT TCATGCCTTG 
TGGACTGCTC TTTACGCTTT CGAAGAAAAG TATGGAAGAT CCCCTGCTCC TCGATCCACT 
CAGGATGCCG CATTGTTAAA AGAATTGATT CCTTCTGGTA CTGAAGAAAT CCCAGAAAAG 
TTGATCGAAT TGTTCTCGTT TTCTGCATCC GGAAACCTCG TCACTGTGTC ATCTGTTGTT 
GGTGGTATTG CTGCACAAGA AGCAATGAAA GGAGTCACAC ACCATATGAC ACCACTGAAA 
CAATGGCTTC ATTTGGATCA CGTTGAAGTC TTGCCAGGGG ATTGGACTTC TTTCGACAAT 
TCCAAACTTT CGGAAACTGA TTGTCAGCCG AGACAATCTC GTTATGATGG ACAAGCTGCC 
GTATTTGGAT GGCCATATCA AGAGTGTCTA TTCCGTCAAA GATGGTTTGT TGTTGGTGCT 
GGAGCTATCG GATGCGAATT GCTCAAAAAT TTATCAATGA TGGGTGTCGC TTGTGGTGAA 
GGAGGTCTTA TCAAAATTAC TGATATGGAC CAAATTGAAA TTTCGAATCT CAACAGACAA 
TTCCTGTTCA GACGCCGTGA CGTTGGGGGA AAGAAGTCAG AATGTGCCGC CCGTGCTGTG 
ACTGCTTTCA ACTCGGATGT TCGAATTGAA GCTTTGGCAG AACGTGTTGG ATTGGAAACC 
GAGCACATTT TCAATGACGA ATTCTTCGGA GAGCTCAATG GAGTTGCCAA TGCGCTTGAT 
AATGTTGATG CTCGGCGATA CATGGATCGT CGATGCGTCT ACTATCGCTT GCCTTTGCTG 
GAGTCTGGAA CCATGGGAAC CAAAGGAAAC ACTCAAGTTG TTTATCCATA TCTCACGGAG 
TCATACTCGT CGTCTGTCGA TCCACCAGAG AAGGAGATTC CTGTTTGCAC GTTGAAGAAC 
TTCCCCAATG AAATTCAGCA CACAATTCAA TGGGCGCGTG AGCAATTTGA AACATTCTTC 
GCCCAGCCAG GAGAAATGGC CAACAAGTTC CTTTCCGATG AAAGAGGGTT CAATGAGCAT 
GTCGACAAAT TAATTTCTGG ACAACAAATT GATATTCTTC AAAAAGTAAA AGACGCTTTG 
ATTGATGCTC GTCCATCGTC TGCTGAAGAT TGTATTCGTT GGGCTCGCAA CCAATTCCAG 
GAACTCTACC ACAACAATAT TGCTCAGATG CTTCACTCTT TCCCACCAGA TCAACTCACT 
GATTCTGGAG CCAAGTTCTG GTCAGGAGCT AAGCGTTGCC CGCATGTTCT CAATTTTGAT 
CCATCCAAAG AAGAACACTT CAATTTCGTT TTTGCTGCGT CAATTCTCAT TGCCGAGTTA 
TACGGAGTTC AACCAATTTT GGACAGAGAA GAAGTTATTC GTGTCGCTCT TTCGGTGAAT 
CCGGAACCTT TCGAGCCTAA ATCCGGTGTC AAGATCGCTG TAACTGACGC CGAGGCTAAA 
GAACAGAATG AACGTGGAGC TTCTAGCATG ATCGTTGATG ATGATGCTGC CATCGAAGCT 
TTGAAATTGA AATTGGCAAC GCTGAACGTC AAGTCTACAA GCAAACTGAA CTGTGTTGAT 
TTTGAGAAGG ATGATGATTC TAACCATCAT ATGGAGTTCA TCACGGCTGC ATCGAATCTC 
CGAGCCGAAA ACTATGATAT TCTTCCAGCT GATAGAATGA GAACCAAACA AATTGCTGGA 
AAAATTATTC CAGCCATCGC TACCACCACC GCAGCAGTTG CCGGTCTTGT TTGCATCGAA 
CTGTACAAGG TTGTTGACGC AAACGGTATT CCTAAGACAC CAATGGAGCG TTTCAAGAAC 
ACTTTCCTAA ATCTGTCAAT GCCATTCTTC TCCTCTGCCG AACCAATTGG CGCTCCAAAG 
AAAACTTACA TGGACCGAGA GTTTACTCTC TGGGATAGAA TTGACGTTCA AGGACCGCTC 
ACACTTCAAG AATTCATCGA CAATGTACAA AACCAAACTG GTGGATGCGA GGTGTCGATG 
TTATCCGCTG GAGCGTGTCT TTTGTTCTCG TTTTTCATGA ATGCTGGCAA AAAACAAGAA 
AGACTCAAAA CAGAAGTGAA AGCGGTTTAC GAGGAATTGC TGAAGAAATC ACTTCATCCT 
TCTGTTCACG CTTTGGTTCT CGAGCCAATG ATGTCCGATC CAGACGGTGA AGATGTCGAA 
GTTCCATACA TTCGCTACTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033405.1
CDS	61..3147
Translation	MDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001038316.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038316.4

ORF Insert Sequence:

ATGGACACTA GCAATAATGC CGGAGGAGTT GGTGGGAACT CAGATGAGCT TCTCGACAAA 
AATCTTTACT CCCGTCAAAT TTACACGCTC GGCGAATCCG CCATGGTCAA CCTCCGAACT 
GCTTCCGTTC TCATTTCTGG TCTCGGTTCT GTAGGAGTCG AAATTGCAAA GAATCTGATT 
CTTGGAGGAG TTCGGCATGT TACTATTCAT GACACAAAGC TTGCAAAATG GTCTGATCTC 
TCGGCTCAAT ACTACTTGCG CGATGCAGAT GTTGGTCACA ATCGTGCTAC TTCTTGCTAC 
GAGCGTCTTG CAGAGCTTAA TGACAGTGTT AATGTACAAG TATCAACTGA TGAGCTCACT 
GAGGAGTTTG TCAAAACATT TGATCTTGTT GTTCTCACGG ACGCTGCCCG TACTGCTCAA 
CGCCAAATCG CTGCATGGAC ACGAGCCCAC AATAGACGTA TTCTGATTAC TGATGCTCGT 
GGTGTATTCT CATACATCTT CAACGATTTT GGAGACAATT TCCGAATTGA TGACGCTACT 
GGGGAGCAAG TTCGCGAGTT CTTCATTGAG CACATTGATA AAACCACTGG TGAAGTGACA 
ACATTGGAGA ATCTTTTCCA TGGTCTTGAG GATGGAGATC ACGTTACTTT TTCTGAAGTT 
AAAGGACTTA CTGAAATTAA TGGATGTGAG CCACTCAAAA TCACCGTTAA AAATGCATCG 
AAATTCAATA TTGGTGATTT CGCAGTTTCA TTCTCCGACT ACAAAGAAGG TGGAAGATGT 
AGGCAAGTTA AAGTCCCAAC ATCGGTATCT CATGTTCCAT TCGAGAAATC TCTTGTTGAA 
CCAGAATTCG GAATATGGGA CTATGCCAAG TTCGAATACC CATCCCAGCT TCATGCCTTG 
TGGACTGCTC TTTACGCTTT CGAAGAAAAG TATGGAAGAT CCCCTGCTCC TCGATCCACT 
CAGGATGCCG CATTGTTAAA AGAATTGATT CCTTCTGGTA CTGAAGAAAT CCCAGAAAAG 
TTGATCGAAT TGTTCTCGTT TTCTGCATCC GGAAACCTCG TCACTGTGTC ATCTGTTGTT 
GGTGGTATTG CTGCACAAGA AGCAATGAAA GGAGTCACAC ACCATATGAC ACCACTGAAA 
CAATGGCTTC ATTTGGATCA CGTTGAAGTC TTGCCAGGGG ATTGGACTTC TTTCGACAAT 
TCCAAACTTT CGGAAACTGA TTGTCAGCCG AGACAATCTC GTTATGATGG ACAAGCTGCC 
GTATTTGGAT GGCCATATCA AGAGTGTCTA TTCCGTCAAA GATGGTTTGT TGTTGGTGCT 
GGAGCTATCG GATGCGAATT GCTCAAAAAT TTATCAATGA TGGGTGTCGC TTGTGGTGAA 
GGAGGTCTTA TCAAAATTAC TGATATGGAC CAAATTGAAA TTTCGAATCT CAACAGACAA 
TTCCTGTTCA GACGCCGTGA CGTTGGGGGA AAGAAGTCAG AATGTGCCGC CCGTGCTGTG 
ACTGCTTTCA ACTCGGATGT TCGAATTGAA GCTTTGGCAG AACGTGTTGG ATTGGAAACC 
GAGCACATTT TCAATGACGA ATTCTTCGGA GAGCTCAATG GAGTTGCCAA TGCGCTTGAT 
AATGTTGATG CTCGGCGATA CATGGATCGT CGATGCGTCT ACTATCGCTT GCCTTTGCTG 
GAGTCTGGAA CCATGGGAAC CAAAGGAAAC ACTCAAGTTG TTTATCCATA TCTCACGGAG 
TCATACTCGT CGTCTGTCGA TCCACCAGAG AAGGAGATTC CTGTTTGCAC GTTGAAGAAC 
TTCCCCAATG AAATTCAGCA CACAATTCAA TGGGCGCGTG AGCAATTTGA AACATTCTTC 
GCCCAGCCAG GAGAAATGGC CAACAAGTTC CTTTCCGATG AAAGAGGGTT CAATGAGCAT 
GTCGACAAAT TAATTTCTGG ACAACAAATT GATATTCTTC AAAAAGTAAA AGACGCTTTG 
ATTGATGCTC GTCCATCGTC TGCTGAAGAT TGTATTCGTT GGGCTCGCAA CCAATTCCAG 
GAACTCTACC ACAACAATAT TGCTCAGATG CTTCACTCTT TCCCACCAGA TCAACTCACT 
GATTCTGGAG CCAAGTTCTG GTCAGGAGCT AAGCGTTGCC CGCATGTTCT CAATTTTGAT 
CCATCCAAAG AAGAACACTT CAATTTCGTT TTTGCTGCGT CAATTCTCAT TGCCGAGTTA 
TACGGAGTTC AACCAATTTT GGACAGAGAA GAAGTTATTC GTGTCGCTCT TTCGGTGAAT 
CCGGAACCTT TCGAGCCTAA ATCCGGTGTC AAGATCGCTG TAACTGACGC CGAGGCTAAA 
GAACAGAATG AACGTGGAGC TTCTAGCATG ATCGTTGATG ATGATGCTGC CATCGAAGCT 
TTGAAATTGA AATTGGCAAC GCTGAACGTC AAGTCTACAA GCAAACTGAA CTGTGTTGAT 
TTTGAGAAGG ATGATGATTC TAACCATCAT ATGGAGTTCA TCACGGCTGC ATCGAATCTC 
CGAGCCGAAA ACTATGATAT TCTTCCAGCT GATAGAATGA GAACCAAACA AATTGCTGGA 
AAAATTATTC CAGCCATCGC TACCACCACC GCAGCAGTTG CCGGTCTTGT TTGCATCGAA 
CTGTACAAGG TTGTTGACGC AAACGGTATT CCTAAGACAC CAATGGAGCG TTTCAAGAAC 
ACTTTCCTAA ATCTGTCAAT GCCATTCTTC TCCTCTGCCG AACCAATTGG CGCTCCAAAG 
AAAACTTACA TGGACCGAGA GTTTACTCTC TGGGATAGAA TTGACGTTCA AGGACCGCTC 
ACACTTCAAG AATTCATCGA CAATGTACAA AACCAAACTG GTGGATGCGA GGTGTCGATG 
TTATCCGCTG GAGCGTGTCT TTTGTTCTCG TTTTTCATGA ATGCTGGCAA AAAACAAGAA 
AGACTCAAAA CAGAAGTGAA AGCGGTTTAC GAGGAATTGC TGAAGAAATC ACTTCATCCT 
TCTGTTCACG CTTTGGTTCT CGAGCCAATG ATGTCCGATC CAGACGGTGA AGATGTCGAA 
GTTCCATACA TTCGCTACTC TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba-1 ( NM_001268520.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255449.1 Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

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CloneID	OCa343554
Clone ID Related Accession (Same CDS sequence)	NM_001268520.2 , NM_001268520.1
Accession Version	NM_001268520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	UBA_e1_C domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268520.1.

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATTCTGG AAACGTGAAC GTCGAGACGA CCAAAAACAC TGAAGGACAA 
GACGGTGAGA AGATGGACAC TAGCAATAAT GCCGGAGGAG TTGGTGGGAA CTCAGATGAG 
CTTCTCGACA AAAATCTTTA CTCCCGTCAA ATTTACACGC TCGGCGAATC CGCCATGGTC 
AACCTCCGAA CTGCTTCCGT TCTCATTTCT GGTCTCGGTT CTGTAGGAGT CGAAATTGCA 
AAGAATCTGA TTCTTGGAGG AGTTCGGCAT GTTACTATTC ATGACACAAA GCTTGCAAAA 
TGGTCTGATC TCTCGGCTCA ATACTACTTG CGCGATGCAG ATGTTGGTCA CAATCGTGCT 
ACTTCTTGCT ACGAGCGTCT TGCAGAGCTT AATGACAGTG TTAATGTACA AGTATCAACT 
GATGAGCTCA CTGAGGAGTT TGTCAAAACA TTTGATCTTG TTGTTCTCAC GGACGCTGCC 
CGTACTGCTC AACGCCAAAT CGCTGCATGG ACACGAGCCC ACAATAGACG TATTCTGATT 
ACTGATGCTC GTGGTGTATT CTCATACATC TTCAACGATT TTGGAGACAA TTTCCGAATT 
GATGACGCTA CTGGGGAGCA AGTTCGCGAG TTCTTCATTG AGCACATTGA TAAAACCACT 
GGTGAAGTGA CAACATTGGA GAATCTTTTC CATGGTCTTG AGGATGGAGA TCACGTTACT 
TTTTCTGAAG TTAAAGGACT TACTGAAATT AATGGATGTG AGCCACTCAA AATCACCGTT 
AAAAATGCAT CGAAATTCAA TATTGGTGAT TTCGCAGTTT CATTCTCCGA CTACAAAGAA 
GGTGGAAGAT GTAGGCAAGT TAAAGTCCCA ACATCGGTAT CTCATGTTCC ATTCGAGAAA 
TCTCTTGTTG AACCAGAATT CGGAATATGG GACTATGCCA AGTTCGAATA CCCATCCCAG 
CTTCATGCCT TGTGGACTGC TCTTTACGCT TTCGAAGAAA AGTATGGAAG ATCCCCTGCT 
CCTCGATCCA CTCAGGATGC CGCATTGTTA AAAGAATTGA TTCCTTCTGG TACTGAAGAA 
ATCCCAGAAA AGTTGATCGA ATTGTTCTCG TTTTCTGCAT CCGGAAACCT CGTCACTGTG 
TCATCTGTTG TTGGTGGTAT TGCTGCACAA GAAGCAATGA AAGGAGTCAC ACACCATATG 
ACACCACTGA AACAATGGCT TCATTTGGAT CACGTTGAAG TCTTGCCAGG GGATTGGACT 
TCTTTCGACA ATTCCAAACT TTCGGAAACT GATTGTCAGC CGAGACAATC TCGTTATGAT 
GGACAAGCTG CCGTATTTGG ATGGCCATAT CAAGAGTGTC TATTCCGTCA AAGATGGTTT 
GTTGTTGGTG CTGGAGCTAT CGGATGCGAA TTGCTCAAAA ATTTATCAAT GATGGGTGTC 
GCTTGTGGTG AAGGAGGTCT TATCAAAATT ACTGATATGG ACCAAATTGA AATTTCGAAT 
CTCAACAGAC AATTCCTGTT CAGACGCCGT GACGTTGGGG GAAAGAAGTC AGAATGTGCC 
GCCCGTGCTG TGACTGCTTT CAACTCGGAT GTTCGAATTG AAGCTTTGGC AGAACGTGTT 
GGATTGGAAA CCGAGCACAT TTTCAATGAC GAATTCTTCG GAGAGCTCAA TGGAGTTGCC 
AATGCGCTTG ATAATGTTGA TGCTCGGCGA TACATGGATC GTCGATGCGT CTACTATCGC 
TTGCCTTTGC TGGAGTCTGG AACCATGGGA ACCAAAGGAA ACACTCAAGT TGTTTATCCA 
TATCTCACGG AGTCATACTC GTCGTCTGTC GATCCACCAG AGAAGGAGAT TCCTGTTTGC 
ACGTTGAAGA ACTTCCCCAA TGAAATTCAG CACACAATTC AATGGGCGCG TGAGCAATTT 
GAAACATTCT TCGCCCAGCC AGGAGAAATG GCCAACAAGT TCCTTTCCGA TGAAAGAGGG 
TTCAATGAGC ATGTCGACAA ATTAATTTCT GGACAACAAA TTGATATTCT TCAAAAAGTA 
AAAGACGCTT TGATTGATGC TCGTCCATCG TCTGCTGAAG ATTGTATTCG TTGGGCTCGC 
AACCAATTCC AGGAACTCTA CCACAACAAT ATTGCTCAGA TGCTTCACTC TTTCCCACCA 
GATCAACTCA CTGATTCTGG AGCCAAGTTC TGGTCAGGAG CTAAGCGTTG CCCGCATGTT 
CTCAATTTTG ATCCATCCAA AGAAGAACAC TTCAATTTCG TTTTTGCTGC GTCAATTCTC 
ATTGCCGAGT TATACGGAGT TCAACCAATT TTGGACAGAG AAGAAGTTAT TCGTGTCGCT 
CTTTCGGTGA ATCCGGAACC TTTCGAGCCT AAATCCGGTG TCAAGATCGC TGTAACTGAC 
GCCGAGGCTA AAGAACAGAA TGAACGTGGA GCTTCTAGCA TGATCGTTGA TGATGATGCT 
GCCATCGAAG CTTTGAAATT GAAATTGGCA ACGCTGAACG TCAAGTCTAC AAGCAAACTG 
AACTGTGTTG ATTTTGAGAA GGATGATGAT TCTAACCATC ATATGGAGTT CATCACGGCT 
GCATCGAATC TCCGAGCCGA AAACTATGAT ATTCTTCCAG CTGATAGAAT GAGAACCAAA 
CAAATTGCTG GAAAAATTAT TCCAGCCATC GCTACCACCA CCGCAGCAGT TGCCGGTCTT 
GTTTGCATCG AACTGTACAA GGTTGTTGAC GCAAACGGTA TTCCTAAGAC ACCAATGGAG 
CGTTTCAAGA ACACTTTCCT AAATCTGTCA ATGCCATTCT TCTCCTCTGC CGAACCAATT 
GGCGCTCCAA AGAAAACTTA CATGGACCGA GAGTTTACTC TCTGGGATAG AATTGACGTT 
CAAGGACCGC TCACACTTCA AGAATTCATC GACAATGTAC AAAACCAAAC TGGTGGATGC 
GAGGTGTCGA TGTTATCCGC TGGAGCGTGT CTTTTGTTCT CGTTTTTCAT GAATGCTGGC 
AAAAAACAAG AAAGACTCAA AACAGAAGTG AAAGCGGTTT ACGAGGAATT GCTGAAGAAA 
TCACTTCATC CTTCTGTTCA CGCTTTGGTT CTCGAGCCAA TGATGTCCGA TCCAGACGGT 
GAAGATGTCG AAGTTCCATA CATTCGCTAC TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255449.1
CDS	23..3361
Translation	MTTILELTSANKTNNKATNNKLQREGENEYPPAKRSKTDDEEEPTVVGNGTIVGAPKQQTLSGVNSGNVNVETTKNTEGQDGEKMDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001268520.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268520.2

ORF Insert Sequence:

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATTCTGG AAACGTGAAC GTCGAGACGA CCAAAAACAC TGAAGGACAA 
GACGGTGAGA AGATGGACAC TAGCAATAAT GCCGGAGGAG TTGGTGGGAA CTCAGATGAG 
CTTCTCGACA AAAATCTTTA CTCCCGTCAA ATTTACACGC TCGGCGAATC CGCCATGGTC 
AACCTCCGAA CTGCTTCCGT TCTCATTTCT GGTCTCGGTT CTGTAGGAGT CGAAATTGCA 
AAGAATCTGA TTCTTGGAGG AGTTCGGCAT GTTACTATTC ATGACACAAA GCTTGCAAAA 
TGGTCTGATC TCTCGGCTCA ATACTACTTG CGCGATGCAG ATGTTGGTCA CAATCGTGCT 
ACTTCTTGCT ACGAGCGTCT TGCAGAGCTT AATGACAGTG TTAATGTACA AGTATCAACT 
GATGAGCTCA CTGAGGAGTT TGTCAAAACA TTTGATCTTG TTGTTCTCAC GGACGCTGCC 
CGTACTGCTC AACGCCAAAT CGCTGCATGG ACACGAGCCC ACAATAGACG TATTCTGATT 
ACTGATGCTC GTGGTGTATT CTCATACATC TTCAACGATT TTGGAGACAA TTTCCGAATT 
GATGACGCTA CTGGGGAGCA AGTTCGCGAG TTCTTCATTG AGCACATTGA TAAAACCACT 
GGTGAAGTGA CAACATTGGA GAATCTTTTC CATGGTCTTG AGGATGGAGA TCACGTTACT 
TTTTCTGAAG TTAAAGGACT TACTGAAATT AATGGATGTG AGCCACTCAA AATCACCGTT 
AAAAATGCAT CGAAATTCAA TATTGGTGAT TTCGCAGTTT CATTCTCCGA CTACAAAGAA 
GGTGGAAGAT GTAGGCAAGT TAAAGTCCCA ACATCGGTAT CTCATGTTCC ATTCGAGAAA 
TCTCTTGTTG AACCAGAATT CGGAATATGG GACTATGCCA AGTTCGAATA CCCATCCCAG 
CTTCATGCCT TGTGGACTGC TCTTTACGCT TTCGAAGAAA AGTATGGAAG ATCCCCTGCT 
CCTCGATCCA CTCAGGATGC CGCATTGTTA AAAGAATTGA TTCCTTCTGG TACTGAAGAA 
ATCCCAGAAA AGTTGATCGA ATTGTTCTCG TTTTCTGCAT CCGGAAACCT CGTCACTGTG 
TCATCTGTTG TTGGTGGTAT TGCTGCACAA GAAGCAATGA AAGGAGTCAC ACACCATATG 
ACACCACTGA AACAATGGCT TCATTTGGAT CACGTTGAAG TCTTGCCAGG GGATTGGACT 
TCTTTCGACA ATTCCAAACT TTCGGAAACT GATTGTCAGC CGAGACAATC TCGTTATGAT 
GGACAAGCTG CCGTATTTGG ATGGCCATAT CAAGAGTGTC TATTCCGTCA AAGATGGTTT 
GTTGTTGGTG CTGGAGCTAT CGGATGCGAA TTGCTCAAAA ATTTATCAAT GATGGGTGTC 
GCTTGTGGTG AAGGAGGTCT TATCAAAATT ACTGATATGG ACCAAATTGA AATTTCGAAT 
CTCAACAGAC AATTCCTGTT CAGACGCCGT GACGTTGGGG GAAAGAAGTC AGAATGTGCC 
GCCCGTGCTG TGACTGCTTT CAACTCGGAT GTTCGAATTG AAGCTTTGGC AGAACGTGTT 
GGATTGGAAA CCGAGCACAT TTTCAATGAC GAATTCTTCG GAGAGCTCAA TGGAGTTGCC 
AATGCGCTTG ATAATGTTGA TGCTCGGCGA TACATGGATC GTCGATGCGT CTACTATCGC 
TTGCCTTTGC TGGAGTCTGG AACCATGGGA ACCAAAGGAA ACACTCAAGT TGTTTATCCA 
TATCTCACGG AGTCATACTC GTCGTCTGTC GATCCACCAG AGAAGGAGAT TCCTGTTTGC 
ACGTTGAAGA ACTTCCCCAA TGAAATTCAG CACACAATTC AATGGGCGCG TGAGCAATTT 
GAAACATTCT TCGCCCAGCC AGGAGAAATG GCCAACAAGT TCCTTTCCGA TGAAAGAGGG 
TTCAATGAGC ATGTCGACAA ATTAATTTCT GGACAACAAA TTGATATTCT TCAAAAAGTA 
AAAGACGCTT TGATTGATGC TCGTCCATCG TCTGCTGAAG ATTGTATTCG TTGGGCTCGC 
AACCAATTCC AGGAACTCTA CCACAACAAT ATTGCTCAGA TGCTTCACTC TTTCCCACCA 
GATCAACTCA CTGATTCTGG AGCCAAGTTC TGGTCAGGAG CTAAGCGTTG CCCGCATGTT 
CTCAATTTTG ATCCATCCAA AGAAGAACAC TTCAATTTCG TTTTTGCTGC GTCAATTCTC 
ATTGCCGAGT TATACGGAGT TCAACCAATT TTGGACAGAG AAGAAGTTAT TCGTGTCGCT 
CTTTCGGTGA ATCCGGAACC TTTCGAGCCT AAATCCGGTG TCAAGATCGC TGTAACTGAC 
GCCGAGGCTA AAGAACAGAA TGAACGTGGA GCTTCTAGCA TGATCGTTGA TGATGATGCT 
GCCATCGAAG CTTTGAAATT GAAATTGGCA ACGCTGAACG TCAAGTCTAC AAGCAAACTG 
AACTGTGTTG ATTTTGAGAA GGATGATGAT TCTAACCATC ATATGGAGTT CATCACGGCT 
GCATCGAATC TCCGAGCCGA AAACTATGAT ATTCTTCCAG CTGATAGAAT GAGAACCAAA 
CAAATTGCTG GAAAAATTAT TCCAGCCATC GCTACCACCA CCGCAGCAGT TGCCGGTCTT 
GTTTGCATCG AACTGTACAA GGTTGTTGAC GCAAACGGTA TTCCTAAGAC ACCAATGGAG 
CGTTTCAAGA ACACTTTCCT AAATCTGTCA ATGCCATTCT TCTCCTCTGC CGAACCAATT 
GGCGCTCCAA AGAAAACTTA CATGGACCGA GAGTTTACTC TCTGGGATAG AATTGACGTT 
CAAGGACCGC TCACACTTCA AGAATTCATC GACAATGTAC AAAACCAAAC TGGTGGATGC 
GAGGTGTCGA TGTTATCCGC TGGAGCGTGT CTTTTGTTCT CGTTTTTCAT GAATGCTGGC 
AAAAAACAAG AAAGACTCAA AACAGAAGTG AAAGCGGTTT ACGAGGAATT GCTGAAGAAA 
TCACTTCATC CTTCTGTTCA CGCTTTGGTT CTCGAGCCAA TGATGTCCGA TCCAGACGGT 
GAAGATGTCG AAGTTCCATA CATTCGCTAC TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba-1 ( NM_001268520.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255449.1 Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

Price & Availability↑

CloneID	OCa343554
Clone ID Related Accession (Same CDS sequence)	NM_001268520.2 , NM_001268520.1
Accession Version	NM_001268520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	UBA (human ubiquitin) related
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATTCTGG AAACGTGAAC GTCGAGACGA CCAAAAACAC TGAAGGACAA 
GACGGTGAGA AGATGGACAC TAGCAATAAT GCCGGAGGAG TTGGTGGGAA CTCAGATGAG 
CTTCTCGACA AAAATCTTTA CTCCCGTCAA ATTTACACGC TCGGCGAATC CGCCATGGTC 
AACCTCCGAA CTGCTTCCGT TCTCATTTCT GGTCTCGGTT CTGTAGGAGT CGAAATTGCA 
AAGAATCTGA TTCTTGGAGG AGTTCGGCAT GTTACTATTC ATGACACAAA GCTTGCAAAA 
TGGTCTGATC TCTCGGCTCA ATACTACTTG CGCGATGCAG ATGTTGGTCA CAATCGTGCT 
ACTTCTTGCT ACGAGCGTCT TGCAGAGCTT AATGACAGTG TTAATGTACA AGTATCAACT 
GATGAGCTCA CTGAGGAGTT TGTCAAAACA TTTGATCTTG TTGTTCTCAC GGACGCTGCC 
CGTACTGCTC AACGCCAAAT CGCTGCATGG ACACGAGCCC ACAATAGACG TATTCTGATT 
ACTGATGCTC GTGGTGTATT CTCATACATC TTCAACGATT TTGGAGACAA TTTCCGAATT 
GATGACGCTA CTGGGGAGCA AGTTCGCGAG TTCTTCATTG AGCACATTGA TAAAACCACT 
GGTGAAGTGA CAACATTGGA GAATCTTTTC CATGGTCTTG AGGATGGAGA TCACGTTACT 
TTTTCTGAAG TTAAAGGACT TACTGAAATT AATGGATGTG AGCCACTCAA AATCACCGTT 
AAAAATGCAT CGAAATTCAA TATTGGTGAT TTCGCAGTTT CATTCTCCGA CTACAAAGAA 
GGTGGAAGAT GTAGGCAAGT TAAAGTCCCA ACATCGGTAT CTCATGTTCC ATTCGAGAAA 
TCTCTTGTTG AACCAGAATT CGGAATATGG GACTATGCCA AGTTCGAATA CCCATCCCAG 
CTTCATGCCT TGTGGACTGC TCTTTACGCT TTCGAAGAAA AGTATGGAAG ATCCCCTGCT 
CCTCGATCCA CTCAGGATGC CGCATTGTTA AAAGAATTGA TTCCTTCTGG TACTGAAGAA 
ATCCCAGAAA AGTTGATCGA ATTGTTCTCG TTTTCTGCAT CCGGAAACCT CGTCACTGTG 
TCATCTGTTG TTGGTGGTAT TGCTGCACAA GAAGCAATGA AAGGAGTCAC ACACCATATG 
ACACCACTGA AACAATGGCT TCATTTGGAT CACGTTGAAG TCTTGCCAGG GGATTGGACT 
TCTTTCGACA ATTCCAAACT TTCGGAAACT GATTGTCAGC CGAGACAATC TCGTTATGAT 
GGACAAGCTG CCGTATTTGG ATGGCCATAT CAAGAGTGTC TATTCCGTCA AAGATGGTTT 
GTTGTTGGTG CTGGAGCTAT CGGATGCGAA TTGCTCAAAA ATTTATCAAT GATGGGTGTC 
GCTTGTGGTG AAGGAGGTCT TATCAAAATT ACTGATATGG ACCAAATTGA AATTTCGAAT 
CTCAACAGAC AATTCCTGTT CAGACGCCGT GACGTTGGGG GAAAGAAGTC AGAATGTGCC 
GCCCGTGCTG TGACTGCTTT CAACTCGGAT GTTCGAATTG AAGCTTTGGC AGAACGTGTT 
GGATTGGAAA CCGAGCACAT TTTCAATGAC GAATTCTTCG GAGAGCTCAA TGGAGTTGCC 
AATGCGCTTG ATAATGTTGA TGCTCGGCGA TACATGGATC GTCGATGCGT CTACTATCGC 
TTGCCTTTGC TGGAGTCTGG AACCATGGGA ACCAAAGGAA ACACTCAAGT TGTTTATCCA 
TATCTCACGG AGTCATACTC GTCGTCTGTC GATCCACCAG AGAAGGAGAT TCCTGTTTGC 
ACGTTGAAGA ACTTCCCCAA TGAAATTCAG CACACAATTC AATGGGCGCG TGAGCAATTT 
GAAACATTCT TCGCCCAGCC AGGAGAAATG GCCAACAAGT TCCTTTCCGA TGAAAGAGGG 
TTCAATGAGC ATGTCGACAA ATTAATTTCT GGACAACAAA TTGATATTCT TCAAAAAGTA 
AAAGACGCTT TGATTGATGC TCGTCCATCG TCTGCTGAAG ATTGTATTCG TTGGGCTCGC 
AACCAATTCC AGGAACTCTA CCACAACAAT ATTGCTCAGA TGCTTCACTC TTTCCCACCA 
GATCAACTCA CTGATTCTGG AGCCAAGTTC TGGTCAGGAG CTAAGCGTTG CCCGCATGTT 
CTCAATTTTG ATCCATCCAA AGAAGAACAC TTCAATTTCG TTTTTGCTGC GTCAATTCTC 
ATTGCCGAGT TATACGGAGT TCAACCAATT TTGGACAGAG AAGAAGTTAT TCGTGTCGCT 
CTTTCGGTGA ATCCGGAACC TTTCGAGCCT AAATCCGGTG TCAAGATCGC TGTAACTGAC 
GCCGAGGCTA AAGAACAGAA TGAACGTGGA GCTTCTAGCA TGATCGTTGA TGATGATGCT 
GCCATCGAAG CTTTGAAATT GAAATTGGCA ACGCTGAACG TCAAGTCTAC AAGCAAACTG 
AACTGTGTTG ATTTTGAGAA GGATGATGAT TCTAACCATC ATATGGAGTT CATCACGGCT 
GCATCGAATC TCCGAGCCGA AAACTATGAT ATTCTTCCAG CTGATAGAAT GAGAACCAAA 
CAAATTGCTG GAAAAATTAT TCCAGCCATC GCTACCACCA CCGCAGCAGT TGCCGGTCTT 
GTTTGCATCG AACTGTACAA GGTTGTTGAC GCAAACGGTA TTCCTAAGAC ACCAATGGAG 
CGTTTCAAGA ACACTTTCCT AAATCTGTCA ATGCCATTCT TCTCCTCTGC CGAACCAATT 
GGCGCTCCAA AGAAAACTTA CATGGACCGA GAGTTTACTC TCTGGGATAG AATTGACGTT 
CAAGGACCGC TCACACTTCA AGAATTCATC GACAATGTAC AAAACCAAAC TGGTGGATGC 
GAGGTGTCGA TGTTATCCGC TGGAGCGTGT CTTTTGTTCT CGTTTTTCAT GAATGCTGGC 
AAAAAACAAG AAAGACTCAA AACAGAAGTG AAAGCGGTTT ACGAGGAATT GCTGAAGAAA 
TCACTTCATC CTTCTGTTCA CGCTTTGGTT CTCGAGCCAA TGATGTCCGA TCCAGACGGT 
GAAGATGTCG AAGTTCCATA CATTCGCTAC TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255449.1
CDS	1..3339
Translation	MTTILELTSANKTNNKATNNKLQREGENEYPPAKRSKTDDEEEPTVVGNGTIVGAPKQQTLSGVNSGNVNVETTKNTEGQDGEKMDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001268520.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268520.1

ORF Insert Sequence:

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATTCTGG AAACGTGAAC GTCGAGACGA CCAAAAACAC TGAAGGACAA 
GACGGTGAGA AGATGGACAC TAGCAATAAT GCCGGAGGAG TTGGTGGGAA CTCAGATGAG 
CTTCTCGACA AAAATCTTTA CTCCCGTCAA ATTTACACGC TCGGCGAATC CGCCATGGTC 
AACCTCCGAA CTGCTTCCGT TCTCATTTCT GGTCTCGGTT CTGTAGGAGT CGAAATTGCA 
AAGAATCTGA TTCTTGGAGG AGTTCGGCAT GTTACTATTC ATGACACAAA GCTTGCAAAA 
TGGTCTGATC TCTCGGCTCA ATACTACTTG CGCGATGCAG ATGTTGGTCA CAATCGTGCT 
ACTTCTTGCT ACGAGCGTCT TGCAGAGCTT AATGACAGTG TTAATGTACA AGTATCAACT 
GATGAGCTCA CTGAGGAGTT TGTCAAAACA TTTGATCTTG TTGTTCTCAC GGACGCTGCC 
CGTACTGCTC AACGCCAAAT CGCTGCATGG ACACGAGCCC ACAATAGACG TATTCTGATT 
ACTGATGCTC GTGGTGTATT CTCATACATC TTCAACGATT TTGGAGACAA TTTCCGAATT 
GATGACGCTA CTGGGGAGCA AGTTCGCGAG TTCTTCATTG AGCACATTGA TAAAACCACT 
GGTGAAGTGA CAACATTGGA GAATCTTTTC CATGGTCTTG AGGATGGAGA TCACGTTACT 
TTTTCTGAAG TTAAAGGACT TACTGAAATT AATGGATGTG AGCCACTCAA AATCACCGTT 
AAAAATGCAT CGAAATTCAA TATTGGTGAT TTCGCAGTTT CATTCTCCGA CTACAAAGAA 
GGTGGAAGAT GTAGGCAAGT TAAAGTCCCA ACATCGGTAT CTCATGTTCC ATTCGAGAAA 
TCTCTTGTTG AACCAGAATT CGGAATATGG GACTATGCCA AGTTCGAATA CCCATCCCAG 
CTTCATGCCT TGTGGACTGC TCTTTACGCT TTCGAAGAAA AGTATGGAAG ATCCCCTGCT 
CCTCGATCCA CTCAGGATGC CGCATTGTTA AAAGAATTGA TTCCTTCTGG TACTGAAGAA 
ATCCCAGAAA AGTTGATCGA ATTGTTCTCG TTTTCTGCAT CCGGAAACCT CGTCACTGTG 
TCATCTGTTG TTGGTGGTAT TGCTGCACAA GAAGCAATGA AAGGAGTCAC ACACCATATG 
ACACCACTGA AACAATGGCT TCATTTGGAT CACGTTGAAG TCTTGCCAGG GGATTGGACT 
TCTTTCGACA ATTCCAAACT TTCGGAAACT GATTGTCAGC CGAGACAATC TCGTTATGAT 
GGACAAGCTG CCGTATTTGG ATGGCCATAT CAAGAGTGTC TATTCCGTCA AAGATGGTTT 
GTTGTTGGTG CTGGAGCTAT CGGATGCGAA TTGCTCAAAA ATTTATCAAT GATGGGTGTC 
GCTTGTGGTG AAGGAGGTCT TATCAAAATT ACTGATATGG ACCAAATTGA AATTTCGAAT 
CTCAACAGAC AATTCCTGTT CAGACGCCGT GACGTTGGGG GAAAGAAGTC AGAATGTGCC 
GCCCGTGCTG TGACTGCTTT CAACTCGGAT GTTCGAATTG AAGCTTTGGC AGAACGTGTT 
GGATTGGAAA CCGAGCACAT TTTCAATGAC GAATTCTTCG GAGAGCTCAA TGGAGTTGCC 
AATGCGCTTG ATAATGTTGA TGCTCGGCGA TACATGGATC GTCGATGCGT CTACTATCGC 
TTGCCTTTGC TGGAGTCTGG AACCATGGGA ACCAAAGGAA ACACTCAAGT TGTTTATCCA 
TATCTCACGG AGTCATACTC GTCGTCTGTC GATCCACCAG AGAAGGAGAT TCCTGTTTGC 
ACGTTGAAGA ACTTCCCCAA TGAAATTCAG CACACAATTC AATGGGCGCG TGAGCAATTT 
GAAACATTCT TCGCCCAGCC AGGAGAAATG GCCAACAAGT TCCTTTCCGA TGAAAGAGGG 
TTCAATGAGC ATGTCGACAA ATTAATTTCT GGACAACAAA TTGATATTCT TCAAAAAGTA 
AAAGACGCTT TGATTGATGC TCGTCCATCG TCTGCTGAAG ATTGTATTCG TTGGGCTCGC 
AACCAATTCC AGGAACTCTA CCACAACAAT ATTGCTCAGA TGCTTCACTC TTTCCCACCA 
GATCAACTCA CTGATTCTGG AGCCAAGTTC TGGTCAGGAG CTAAGCGTTG CCCGCATGTT 
CTCAATTTTG ATCCATCCAA AGAAGAACAC TTCAATTTCG TTTTTGCTGC GTCAATTCTC 
ATTGCCGAGT TATACGGAGT TCAACCAATT TTGGACAGAG AAGAAGTTAT TCGTGTCGCT 
CTTTCGGTGA ATCCGGAACC TTTCGAGCCT AAATCCGGTG TCAAGATCGC TGTAACTGAC 
GCCGAGGCTA AAGAACAGAA TGAACGTGGA GCTTCTAGCA TGATCGTTGA TGATGATGCT 
GCCATCGAAG CTTTGAAATT GAAATTGGCA ACGCTGAACG TCAAGTCTAC AAGCAAACTG 
AACTGTGTTG ATTTTGAGAA GGATGATGAT TCTAACCATC ATATGGAGTT CATCACGGCT 
GCATCGAATC TCCGAGCCGA AAACTATGAT ATTCTTCCAG CTGATAGAAT GAGAACCAAA 
CAAATTGCTG GAAAAATTAT TCCAGCCATC GCTACCACCA CCGCAGCAGT TGCCGGTCTT 
GTTTGCATCG AACTGTACAA GGTTGTTGAC GCAAACGGTA TTCCTAAGAC ACCAATGGAG 
CGTTTCAAGA ACACTTTCCT AAATCTGTCA ATGCCATTCT TCTCCTCTGC CGAACCAATT 
GGCGCTCCAA AGAAAACTTA CATGGACCGA GAGTTTACTC TCTGGGATAG AATTGACGTT 
CAAGGACCGC TCACACTTCA AGAATTCATC GACAATGTAC AAAACCAAAC TGGTGGATGC 
GAGGTGTCGA TGTTATCCGC TGGAGCGTGT CTTTTGTTCT CGTTTTTCAT GAATGCTGGC 
AAAAAACAAG AAAGACTCAA AACAGAAGTG AAAGCGGTTT ACGAGGAATT GCTGAAGAAA 
TCACTTCATC CTTCTGTTCA CGCTTTGGTT CTCGAGCCAA TGATGTCCGA TCCAGACGGT 
GAAGATGTCG AAGTTCCATA CATTCGCTAC TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba-1 ( NM_001038315.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001033404.1 Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

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CloneID	OCa344502
Clone ID Related Accession (Same CDS sequence)	NM_001038315.5 , NM_001038315.6
Accession Version	NM_001038315.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	UBA (human ubiquitin) related
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001038315.4. COMPLETENESS: incomplete on both ends.

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATCAGTC TGGAAACGTG AACGTCGAGA CGACCAAAAA CACTGAAGGA 
CAAGACGGTG AGAAGATGGA CACTAGCAAT AATGCCGGAG GAGTTGGTGG GAACTCAGAT 
GAGCTTCTCG ACAAAAATCT TTACTCCCGT CAAATTTACA CGCTCGGCGA ATCCGCCATG 
GTCAACCTCC GAACTGCTTC CGTTCTCATT TCTGGTCTCG GTTCTGTAGG AGTCGAAATT 
GCAAAGAATC TGATTCTTGG AGGAGTTCGG CATGTTACTA TTCATGACAC AAAGCTTGCA 
AAATGGTCTG ATCTCTCGGC TCAATACTAC TTGCGCGATG CAGATGTTGG TCACAATCGT 
GCTACTTCTT GCTACGAGCG TCTTGCAGAG CTTAATGACA GTGTTAATGT ACAAGTATCA 
ACTGATGAGC TCACTGAGGA GTTTGTCAAA ACATTTGATC TTGTTGTTCT CACGGACGCT 
GCCCGTACTG CTCAACGCCA AATCGCTGCA TGGACACGAG CCCACAATAG ACGTATTCTG 
ATTACTGATG CTCGTGGTGT ATTCTCATAC ATCTTCAACG ATTTTGGAGA CAATTTCCGA 
ATTGATGACG CTACTGGGGA GCAAGTTCGC GAGTTCTTCA TTGAGCACAT TGATAAAACC 
ACTGGTGAAG TGACAACATT GGAGAATCTT TTCCATGGTC TTGAGGATGG AGATCACGTT 
ACTTTTTCTG AAGTTAAAGG ACTTACTGAA ATTAATGGAT GTGAGCCACT CAAAATCACC 
GTTAAAAATG CATCGAAATT CAATATTGGT GATTTCGCAG TTTCATTCTC CGACTACAAA 
GAAGGTGGAA GATGTAGGCA AGTTAAAGTC CCAACATCGG TATCTCATGT TCCATTCGAG 
AAATCTCTTG TTGAACCAGA ATTCGGAATA TGGGACTATG CCAAGTTCGA ATACCCATCC 
CAGCTTCATG CCTTGTGGAC TGCTCTTTAC GCTTTCGAAG AAAAGTATGG AAGATCCCCT 
GCTCCTCGAT CCACTCAGGA TGCCGCATTG TTAAAAGAAT TGATTCCTTC TGGTACTGAA 
GAAATCCCAG AAAAGTTGAT CGAATTGTTC TCGTTTTCTG CATCCGGAAA CCTCGTCACT 
GTGTCATCTG TTGTTGGTGG TATTGCTGCA CAAGAAGCAA TGAAAGGAGT CACACACCAT 
ATGACACCAC TGAAACAATG GCTTCATTTG GATCACGTTG AAGTCTTGCC AGGGGATTGG 
ACTTCTTTCG ACAATTCCAA ACTTTCGGAA ACTGATTGTC AGCCGAGACA ATCTCGTTAT 
GATGGACAAG CTGCCGTATT TGGATGGCCA TATCAAGAGT GTCTATTCCG TCAAAGATGG 
TTTGTTGTTG GTGCTGGAGC TATCGGATGC GAATTGCTCA AAAATTTATC AATGATGGGT 
GTCGCTTGTG GTGAAGGAGG TCTTATCAAA ATTACTGATA TGGACCAAAT TGAAATTTCG 
AATCTCAACA GACAATTCCT GTTCAGACGC CGTGACGTTG GGGGAAAGAA GTCAGAATGT 
GCCGCCCGTG CTGTGACTGC TTTCAACTCG GATGTTCGAA TTGAAGCTTT GGCAGAACGT 
GTTGGATTGG AAACCGAGCA CATTTTCAAT GACGAATTCT TCGGAGAGCT CAATGGAGTT 
GCCAATGCGC TTGATAATGT TGATGCTCGG CGATACATGG ATCGTCGATG CGTCTACTAT 
CGCTTGCCTT TGCTGGAGTC TGGAACCATG GGAACCAAAG GAAACACTCA AGTTGTTTAT 
CCATATCTCA CGGAGTCATA CTCGTCGTCT GTCGATCCAC CAGAGAAGGA GATTCCTGTT 
TGCACGTTGA AGAACTTCCC CAATGAAATT CAGCACACAA TTCAATGGGC GCGTGAGCAA 
TTTGAAACAT TCTTCGCCCA GCCAGGAGAA ATGGCCAACA AGTTCCTTTC CGATGAAAGA 
GGGTTCAATG AGCATGTCGA CAAATTAATT TCTGGACAAC AAATTGATAT TCTTCAAAAA 
GTAAAAGACG CTTTGATTGA TGCTCGTCCA TCGTCTGCTG AAGATTGTAT TCGTTGGGCT 
CGCAACCAAT TCCAGGAACT CTACCACAAC AATATTGCTC AGATGCTTCA CTCTTTCCCA 
CCAGATCAAC TCACTGATTC TGGAGCCAAG TTCTGGTCAG GAGCTAAGCG TTGCCCGCAT 
GTTCTCAATT TTGATCCATC CAAAGAAGAA CACTTCAATT TCGTTTTTGC TGCGTCAATT 
CTCATTGCCG AGTTATACGG AGTTCAACCA ATTTTGGACA GAGAAGAAGT TATTCGTGTC 
GCTCTTTCGG TGAATCCGGA ACCTTTCGAG CCTAAATCCG GTGTCAAGAT CGCTGTAACT 
GACGCCGAGG CTAAAGAACA GAATGAACGT GGAGCTTCTA GCATGATCGT TGATGATGAT 
GCTGCCATCG AAGCTTTGAA ATTGAAATTG GCAACGCTGA ACGTCAAGTC TACAAGCAAA 
CTGAACTGTG TTGATTTTGA GAAGGATGAT GATTCTAACC ATCATATGGA GTTCATCACG 
GCTGCATCGA ATCTCCGAGC CGAAAACTAT GATATTCTTC CAGCTGATAG AATGAGAACC 
AAACAAATTG CTGGAAAAAT TATTCCAGCC ATCGCTACCA CCACCGCAGC AGTTGCCGGT 
CTTGTTTGCA TCGAACTGTA CAAGGTTGTT GACGCAAACG GTATTCCTAA GACACCAATG 
GAGCGTTTCA AGAACACTTT CCTAAATCTG TCAATGCCAT TCTTCTCCTC TGCCGAACCA 
ATTGGCGCTC CAAAGAAAAC TTACATGGAC CGAGAGTTTA CTCTCTGGGA TAGAATTGAC 
GTTCAAGGAC CGCTCACACT TCAAGAATTC ATCGACAATG TACAAAACCA AACTGGTGGA 
TGCGAGGTGT CGATGTTATC CGCTGGAGCG TGTCTTTTGT TCTCGTTTTT CATGAATGCT 
GGCAAAAAAC AAGAAAGACT CAAAACAGAA GTGAAAGCGG TTTACGAGGA ATTGCTGAAG 
AAATCACTTC ATCCTTCTGT TCACGCTTTG GTTCTCGAGC CAATGATGTC CGATCCAGAC 
GGTGAAGATG TCGAAGTTCC ATACATTCGC TACTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033404.1
CDS	1..3342
Translation	MTTILELTSANKTNNKATNNKLQREGENEYPPAKRSKTDDEEEPTVVGNGTIVGAPKQQTLSGVNQSGNVNVETTKNTEGQDGEKMDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001038315.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA (human ubiquitin) related (uba-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038315.5

ORF Insert Sequence:

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATCAGTC TGGAAACGTG AACGTCGAGA CGACCAAAAA CACTGAAGGA 
CAAGACGGTG AGAAGATGGA CACTAGCAAT AATGCCGGAG GAGTTGGTGG GAACTCAGAT 
GAGCTTCTCG ACAAAAATCT TTACTCCCGT CAAATTTACA CGCTCGGCGA ATCCGCCATG 
GTCAACCTCC GAACTGCTTC CGTTCTCATT TCTGGTCTCG GTTCTGTAGG AGTCGAAATT 
GCAAAGAATC TGATTCTTGG AGGAGTTCGG CATGTTACTA TTCATGACAC AAAGCTTGCA 
AAATGGTCTG ATCTCTCGGC TCAATACTAC TTGCGCGATG CAGATGTTGG TCACAATCGT 
GCTACTTCTT GCTACGAGCG TCTTGCAGAG CTTAATGACA GTGTTAATGT ACAAGTATCA 
ACTGATGAGC TCACTGAGGA GTTTGTCAAA ACATTTGATC TTGTTGTTCT CACGGACGCT 
GCCCGTACTG CTCAACGCCA AATCGCTGCA TGGACACGAG CCCACAATAG ACGTATTCTG 
ATTACTGATG CTCGTGGTGT ATTCTCATAC ATCTTCAACG ATTTTGGAGA CAATTTCCGA 
ATTGATGACG CTACTGGGGA GCAAGTTCGC GAGTTCTTCA TTGAGCACAT TGATAAAACC 
ACTGGTGAAG TGACAACATT GGAGAATCTT TTCCATGGTC TTGAGGATGG AGATCACGTT 
ACTTTTTCTG AAGTTAAAGG ACTTACTGAA ATTAATGGAT GTGAGCCACT CAAAATCACC 
GTTAAAAATG CATCGAAATT CAATATTGGT GATTTCGCAG TTTCATTCTC CGACTACAAA 
GAAGGTGGAA GATGTAGGCA AGTTAAAGTC CCAACATCGG TATCTCATGT TCCATTCGAG 
AAATCTCTTG TTGAACCAGA ATTCGGAATA TGGGACTATG CCAAGTTCGA ATACCCATCC 
CAGCTTCATG CCTTGTGGAC TGCTCTTTAC GCTTTCGAAG AAAAGTATGG AAGATCCCCT 
GCTCCTCGAT CCACTCAGGA TGCCGCATTG TTAAAAGAAT TGATTCCTTC TGGTACTGAA 
GAAATCCCAG AAAAGTTGAT CGAATTGTTC TCGTTTTCTG CATCCGGAAA CCTCGTCACT 
GTGTCATCTG TTGTTGGTGG TATTGCTGCA CAAGAAGCAA TGAAAGGAGT CACACACCAT 
ATGACACCAC TGAAACAATG GCTTCATTTG GATCACGTTG AAGTCTTGCC AGGGGATTGG 
ACTTCTTTCG ACAATTCCAA ACTTTCGGAA ACTGATTGTC AGCCGAGACA ATCTCGTTAT 
GATGGACAAG CTGCCGTATT TGGATGGCCA TATCAAGAGT GTCTATTCCG TCAAAGATGG 
TTTGTTGTTG GTGCTGGAGC TATCGGATGC GAATTGCTCA AAAATTTATC AATGATGGGT 
GTCGCTTGTG GTGAAGGAGG TCTTATCAAA ATTACTGATA TGGACCAAAT TGAAATTTCG 
AATCTCAACA GACAATTCCT GTTCAGACGC CGTGACGTTG GGGGAAAGAA GTCAGAATGT 
GCCGCCCGTG CTGTGACTGC TTTCAACTCG GATGTTCGAA TTGAAGCTTT GGCAGAACGT 
GTTGGATTGG AAACCGAGCA CATTTTCAAT GACGAATTCT TCGGAGAGCT CAATGGAGTT 
GCCAATGCGC TTGATAATGT TGATGCTCGG CGATACATGG ATCGTCGATG CGTCTACTAT 
CGCTTGCCTT TGCTGGAGTC TGGAACCATG GGAACCAAAG GAAACACTCA AGTTGTTTAT 
CCATATCTCA CGGAGTCATA CTCGTCGTCT GTCGATCCAC CAGAGAAGGA GATTCCTGTT 
TGCACGTTGA AGAACTTCCC CAATGAAATT CAGCACACAA TTCAATGGGC GCGTGAGCAA 
TTTGAAACAT TCTTCGCCCA GCCAGGAGAA ATGGCCAACA AGTTCCTTTC CGATGAAAGA 
GGGTTCAATG AGCATGTCGA CAAATTAATT TCTGGACAAC AAATTGATAT TCTTCAAAAA 
GTAAAAGACG CTTTGATTGA TGCTCGTCCA TCGTCTGCTG AAGATTGTAT TCGTTGGGCT 
CGCAACCAAT TCCAGGAACT CTACCACAAC AATATTGCTC AGATGCTTCA CTCTTTCCCA 
CCAGATCAAC TCACTGATTC TGGAGCCAAG TTCTGGTCAG GAGCTAAGCG TTGCCCGCAT 
GTTCTCAATT TTGATCCATC CAAAGAAGAA CACTTCAATT TCGTTTTTGC TGCGTCAATT 
CTCATTGCCG AGTTATACGG AGTTCAACCA ATTTTGGACA GAGAAGAAGT TATTCGTGTC 
GCTCTTTCGG TGAATCCGGA ACCTTTCGAG CCTAAATCCG GTGTCAAGAT CGCTGTAACT 
GACGCCGAGG CTAAAGAACA GAATGAACGT GGAGCTTCTA GCATGATCGT TGATGATGAT 
GCTGCCATCG AAGCTTTGAA ATTGAAATTG GCAACGCTGA ACGTCAAGTC TACAAGCAAA 
CTGAACTGTG TTGATTTTGA GAAGGATGAT GATTCTAACC ATCATATGGA GTTCATCACG 
GCTGCATCGA ATCTCCGAGC CGAAAACTAT GATATTCTTC CAGCTGATAG AATGAGAACC 
AAACAAATTG CTGGAAAAAT TATTCCAGCC ATCGCTACCA CCACCGCAGC AGTTGCCGGT 
CTTGTTTGCA TCGAACTGTA CAAGGTTGTT GACGCAAACG GTATTCCTAA GACACCAATG 
GAGCGTTTCA AGAACACTTT CCTAAATCTG TCAATGCCAT TCTTCTCCTC TGCCGAACCA 
ATTGGCGCTC CAAAGAAAAC TTACATGGAC CGAGAGTTTA CTCTCTGGGA TAGAATTGAC 
GTTCAAGGAC CGCTCACACT TCAAGAATTC ATCGACAATG TACAAAACCA AACTGGTGGA 
TGCGAGGTGT CGATGTTATC CGCTGGAGCG TGTCTTTTGT TCTCGTTTTT CATGAATGCT 
GGCAAAAAAC AAGAAAGACT CAAAACAGAA GTGAAAGCGG TTTACGAGGA ATTGCTGAAG 
AAATCACTTC ATCCTTCTGT TCACGCTTTG GTTCTCGAGC CAATGATGTC CGATCCAGAC 
GGTGAAGATG TCGAAGTTCC ATACATTCGC TACTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

uba-1 ( NM_001038315.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001033404.1 Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

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CloneID	OCa344502
Clone ID Related Accession (Same CDS sequence)	NM_001038315.5 , NM_001038315.6
Accession Version	NM_001038315.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	UBA_e1_C domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001038315.5.

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATCAGTC TGGAAACGTG AACGTCGAGA CGACCAAAAA CACTGAAGGA 
CAAGACGGTG AGAAGATGGA CACTAGCAAT AATGCCGGAG GAGTTGGTGG GAACTCAGAT 
GAGCTTCTCG ACAAAAATCT TTACTCCCGT CAAATTTACA CGCTCGGCGA ATCCGCCATG 
GTCAACCTCC GAACTGCTTC CGTTCTCATT TCTGGTCTCG GTTCTGTAGG AGTCGAAATT 
GCAAAGAATC TGATTCTTGG AGGAGTTCGG CATGTTACTA TTCATGACAC AAAGCTTGCA 
AAATGGTCTG ATCTCTCGGC TCAATACTAC TTGCGCGATG CAGATGTTGG TCACAATCGT 
GCTACTTCTT GCTACGAGCG TCTTGCAGAG CTTAATGACA GTGTTAATGT ACAAGTATCA 
ACTGATGAGC TCACTGAGGA GTTTGTCAAA ACATTTGATC TTGTTGTTCT CACGGACGCT 
GCCCGTACTG CTCAACGCCA AATCGCTGCA TGGACACGAG CCCACAATAG ACGTATTCTG 
ATTACTGATG CTCGTGGTGT ATTCTCATAC ATCTTCAACG ATTTTGGAGA CAATTTCCGA 
ATTGATGACG CTACTGGGGA GCAAGTTCGC GAGTTCTTCA TTGAGCACAT TGATAAAACC 
ACTGGTGAAG TGACAACATT GGAGAATCTT TTCCATGGTC TTGAGGATGG AGATCACGTT 
ACTTTTTCTG AAGTTAAAGG ACTTACTGAA ATTAATGGAT GTGAGCCACT CAAAATCACC 
GTTAAAAATG CATCGAAATT CAATATTGGT GATTTCGCAG TTTCATTCTC CGACTACAAA 
GAAGGTGGAA GATGTAGGCA AGTTAAAGTC CCAACATCGG TATCTCATGT TCCATTCGAG 
AAATCTCTTG TTGAACCAGA ATTCGGAATA TGGGACTATG CCAAGTTCGA ATACCCATCC 
CAGCTTCATG CCTTGTGGAC TGCTCTTTAC GCTTTCGAAG AAAAGTATGG AAGATCCCCT 
GCTCCTCGAT CCACTCAGGA TGCCGCATTG TTAAAAGAAT TGATTCCTTC TGGTACTGAA 
GAAATCCCAG AAAAGTTGAT CGAATTGTTC TCGTTTTCTG CATCCGGAAA CCTCGTCACT 
GTGTCATCTG TTGTTGGTGG TATTGCTGCA CAAGAAGCAA TGAAAGGAGT CACACACCAT 
ATGACACCAC TGAAACAATG GCTTCATTTG GATCACGTTG AAGTCTTGCC AGGGGATTGG 
ACTTCTTTCG ACAATTCCAA ACTTTCGGAA ACTGATTGTC AGCCGAGACA ATCTCGTTAT 
GATGGACAAG CTGCCGTATT TGGATGGCCA TATCAAGAGT GTCTATTCCG TCAAAGATGG 
TTTGTTGTTG GTGCTGGAGC TATCGGATGC GAATTGCTCA AAAATTTATC AATGATGGGT 
GTCGCTTGTG GTGAAGGAGG TCTTATCAAA ATTACTGATA TGGACCAAAT TGAAATTTCG 
AATCTCAACA GACAATTCCT GTTCAGACGC CGTGACGTTG GGGGAAAGAA GTCAGAATGT 
GCCGCCCGTG CTGTGACTGC TTTCAACTCG GATGTTCGAA TTGAAGCTTT GGCAGAACGT 
GTTGGATTGG AAACCGAGCA CATTTTCAAT GACGAATTCT TCGGAGAGCT CAATGGAGTT 
GCCAATGCGC TTGATAATGT TGATGCTCGG CGATACATGG ATCGTCGATG CGTCTACTAT 
CGCTTGCCTT TGCTGGAGTC TGGAACCATG GGAACCAAAG GAAACACTCA AGTTGTTTAT 
CCATATCTCA CGGAGTCATA CTCGTCGTCT GTCGATCCAC CAGAGAAGGA GATTCCTGTT 
TGCACGTTGA AGAACTTCCC CAATGAAATT CAGCACACAA TTCAATGGGC GCGTGAGCAA 
TTTGAAACAT TCTTCGCCCA GCCAGGAGAA ATGGCCAACA AGTTCCTTTC CGATGAAAGA 
GGGTTCAATG AGCATGTCGA CAAATTAATT TCTGGACAAC AAATTGATAT TCTTCAAAAA 
GTAAAAGACG CTTTGATTGA TGCTCGTCCA TCGTCTGCTG AAGATTGTAT TCGTTGGGCT 
CGCAACCAAT TCCAGGAACT CTACCACAAC AATATTGCTC AGATGCTTCA CTCTTTCCCA 
CCAGATCAAC TCACTGATTC TGGAGCCAAG TTCTGGTCAG GAGCTAAGCG TTGCCCGCAT 
GTTCTCAATT TTGATCCATC CAAAGAAGAA CACTTCAATT TCGTTTTTGC TGCGTCAATT 
CTCATTGCCG AGTTATACGG AGTTCAACCA ATTTTGGACA GAGAAGAAGT TATTCGTGTC 
GCTCTTTCGG TGAATCCGGA ACCTTTCGAG CCTAAATCCG GTGTCAAGAT CGCTGTAACT 
GACGCCGAGG CTAAAGAACA GAATGAACGT GGAGCTTCTA GCATGATCGT TGATGATGAT 
GCTGCCATCG AAGCTTTGAA ATTGAAATTG GCAACGCTGA ACGTCAAGTC TACAAGCAAA 
CTGAACTGTG TTGATTTTGA GAAGGATGAT GATTCTAACC ATCATATGGA GTTCATCACG 
GCTGCATCGA ATCTCCGAGC CGAAAACTAT GATATTCTTC CAGCTGATAG AATGAGAACC 
AAACAAATTG CTGGAAAAAT TATTCCAGCC ATCGCTACCA CCACCGCAGC AGTTGCCGGT 
CTTGTTTGCA TCGAACTGTA CAAGGTTGTT GACGCAAACG GTATTCCTAA GACACCAATG 
GAGCGTTTCA AGAACACTTT CCTAAATCTG TCAATGCCAT TCTTCTCCTC TGCCGAACCA 
ATTGGCGCTC CAAAGAAAAC TTACATGGAC CGAGAGTTTA CTCTCTGGGA TAGAATTGAC 
GTTCAAGGAC CGCTCACACT TCAAGAATTC ATCGACAATG TACAAAACCA AACTGGTGGA 
TGCGAGGTGT CGATGTTATC CGCTGGAGCG TGTCTTTTGT TCTCGTTTTT CATGAATGCT 
GGCAAAAAAC AAGAAAGACT CAAAACAGAA GTGAAAGCGG TTTACGAGGA ATTGCTGAAG 
AAATCACTTC ATCCTTCTGT TCACGCTTTG GTTCTCGAGC CAATGATGTC CGATCCAGAC 
GGTGAAGATG TCGAAGTTCC ATACATTCGC TACTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033404.1
CDS	23..3364
Translation	MTTILELTSANKTNNKATNNKLQREGENEYPPAKRSKTDDEEEPTVVGNGTIVGAPKQQTLSGVNQSGNVNVETTKNTEGQDGEKMDTSNNAGGVGGNSDELLDKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVSTDELTEEFVKTFDLVVLTDAARTAQRQIAAWTRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVREFFIEHIDKTTGEVTTLENLFHGLEDGDHVTFSEVKGLTEINGCEPLKITVKNASKFNIGDFAVSFSDYKEGGRCRQVKVPTSVSHVPFEKSLVEPEFGIWDYAKFEYPSQLHALWTALYAFEEKYGRSPAPRSTQDAALLKELIPSGTEEIPEKLIELFSFSASGNLVTVSSVVGGIAAQEAMKGVTHHMTPLKQWLHLDHVEVLPGDWTSFDNSKLSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGGLIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAARAVTAFNSDVRIEALAERVGLETEHIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTESYSSSVDPPEKEIPVCTLKNFPNEIQHTIQWAREQFETFFAQPGEMANKFLSDERGFNEHVDKLISGQQIDILQKVKDALIDARPSSAEDCIRWARNQFQELYHNNIAQMLHSFPPDQLTDSGAKFWSGAKRCPHVLNFDPSKEEHFNFVFAASILIAELYGVQPILDREEVIRVALSVNPEPFEPKSGVKIAVTDAEAKEQNERGASSMIVDDDAAIEALKLKLATLNVKSTSKLNCVDFEKDDDSNHHMEFITAASNLRAENYDILPADRMRTKQIAGKIIPAIATTTAAVAGLVCIELYKVVDANGIPKTPMERFKNTFLNLSMPFFSSAEPIGAPKKTYMDREFTLWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAVYEELLKKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYSF

Target ORF information:

RefSeq Version	NM_001038315.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans UBA_e1_C domain-containing protein (uba-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038315.6

ORF Insert Sequence:

ATGACTACCA TCCTTGAGCT AACATCGGCC AACAAAACTA ACAACAAGGC CACCAATAAC 
AAGCTGCAGC GTGAAGGAGA AAACGAATAT CCTCCGGCGA AACGATCCAA GACTGACGAC 
GAAGAAGAAC CAACAGTCGT AGGAAACGGA ACGATCGTCG GAGCGCCAAA GCAGCAAACA 
CTTTCTGGGG TCAATCAGTC TGGAAACGTG AACGTCGAGA CGACCAAAAA CACTGAAGGA 
CAAGACGGTG AGAAGATGGA CACTAGCAAT AATGCCGGAG GAGTTGGTGG GAACTCAGAT 
GAGCTTCTCG ACAAAAATCT TTACTCCCGT CAAATTTACA CGCTCGGCGA ATCCGCCATG 
GTCAACCTCC GAACTGCTTC CGTTCTCATT TCTGGTCTCG GTTCTGTAGG AGTCGAAATT 
GCAAAGAATC TGATTCTTGG AGGAGTTCGG CATGTTACTA TTCATGACAC AAAGCTTGCA 
AAATGGTCTG ATCTCTCGGC TCAATACTAC TTGCGCGATG CAGATGTTGG TCACAATCGT 
GCTACTTCTT GCTACGAGCG TCTTGCAGAG CTTAATGACA GTGTTAATGT ACAAGTATCA 
ACTGATGAGC TCACTGAGGA GTTTGTCAAA ACATTTGATC TTGTTGTTCT CACGGACGCT 
GCCCGTACTG CTCAACGCCA AATCGCTGCA TGGACACGAG CCCACAATAG ACGTATTCTG 
ATTACTGATG CTCGTGGTGT ATTCTCATAC ATCTTCAACG ATTTTGGAGA CAATTTCCGA 
ATTGATGACG CTACTGGGGA GCAAGTTCGC GAGTTCTTCA TTGAGCACAT TGATAAAACC 
ACTGGTGAAG TGACAACATT GGAGAATCTT TTCCATGGTC TTGAGGATGG AGATCACGTT 
ACTTTTTCTG AAGTTAAAGG ACTTACTGAA ATTAATGGAT GTGAGCCACT CAAAATCACC 
GTTAAAAATG CATCGAAATT CAATATTGGT GATTTCGCAG TTTCATTCTC CGACTACAAA 
GAAGGTGGAA GATGTAGGCA AGTTAAAGTC CCAACATCGG TATCTCATGT TCCATTCGAG 
AAATCTCTTG TTGAACCAGA ATTCGGAATA TGGGACTATG CCAAGTTCGA ATACCCATCC 
CAGCTTCATG CCTTGTGGAC TGCTCTTTAC GCTTTCGAAG AAAAGTATGG AAGATCCCCT 
GCTCCTCGAT CCACTCAGGA TGCCGCATTG TTAAAAGAAT TGATTCCTTC TGGTACTGAA 
GAAATCCCAG AAAAGTTGAT CGAATTGTTC TCGTTTTCTG CATCCGGAAA CCTCGTCACT 
GTGTCATCTG TTGTTGGTGG TATTGCTGCA CAAGAAGCAA TGAAAGGAGT CACACACCAT 
ATGACACCAC TGAAACAATG GCTTCATTTG GATCACGTTG AAGTCTTGCC AGGGGATTGG 
ACTTCTTTCG ACAATTCCAA ACTTTCGGAA ACTGATTGTC AGCCGAGACA ATCTCGTTAT 
GATGGACAAG CTGCCGTATT TGGATGGCCA TATCAAGAGT GTCTATTCCG TCAAAGATGG 
TTTGTTGTTG GTGCTGGAGC TATCGGATGC GAATTGCTCA AAAATTTATC AATGATGGGT 
GTCGCTTGTG GTGAAGGAGG TCTTATCAAA ATTACTGATA TGGACCAAAT TGAAATTTCG 
AATCTCAACA GACAATTCCT GTTCAGACGC CGTGACGTTG GGGGAAAGAA GTCAGAATGT 
GCCGCCCGTG CTGTGACTGC TTTCAACTCG GATGTTCGAA TTGAAGCTTT GGCAGAACGT 
GTTGGATTGG AAACCGAGCA CATTTTCAAT GACGAATTCT TCGGAGAGCT CAATGGAGTT 
GCCAATGCGC TTGATAATGT TGATGCTCGG CGATACATGG ATCGTCGATG CGTCTACTAT 
CGCTTGCCTT TGCTGGAGTC TGGAACCATG GGAACCAAAG GAAACACTCA AGTTGTTTAT 
CCATATCTCA CGGAGTCATA CTCGTCGTCT GTCGATCCAC CAGAGAAGGA GATTCCTGTT 
TGCACGTTGA AGAACTTCCC CAATGAAATT CAGCACACAA TTCAATGGGC GCGTGAGCAA 
TTTGAAACAT TCTTCGCCCA GCCAGGAGAA ATGGCCAACA AGTTCCTTTC CGATGAAAGA 
GGGTTCAATG AGCATGTCGA CAAATTAATT TCTGGACAAC AAATTGATAT TCTTCAAAAA 
GTAAAAGACG CTTTGATTGA TGCTCGTCCA TCGTCTGCTG AAGATTGTAT TCGTTGGGCT 
CGCAACCAAT TCCAGGAACT CTACCACAAC AATATTGCTC AGATGCTTCA CTCTTTCCCA 
CCAGATCAAC TCACTGATTC TGGAGCCAAG TTCTGGTCAG GAGCTAAGCG TTGCCCGCAT 
GTTCTCAATT TTGATCCATC CAAAGAAGAA CACTTCAATT TCGTTTTTGC TGCGTCAATT 
CTCATTGCCG AGTTATACGG AGTTCAACCA ATTTTGGACA GAGAAGAAGT TATTCGTGTC 
GCTCTTTCGG TGAATCCGGA ACCTTTCGAG CCTAAATCCG GTGTCAAGAT CGCTGTAACT 
GACGCCGAGG CTAAAGAACA GAATGAACGT GGAGCTTCTA GCATGATCGT TGATGATGAT 
GCTGCCATCG AAGCTTTGAA ATTGAAATTG GCAACGCTGA ACGTCAAGTC TACAAGCAAA 
CTGAACTGTG TTGATTTTGA GAAGGATGAT GATTCTAACC ATCATATGGA GTTCATCACG 
GCTGCATCGA ATCTCCGAGC CGAAAACTAT GATATTCTTC CAGCTGATAG AATGAGAACC 
AAACAAATTG CTGGAAAAAT TATTCCAGCC ATCGCTACCA CCACCGCAGC AGTTGCCGGT 
CTTGTTTGCA TCGAACTGTA CAAGGTTGTT GACGCAAACG GTATTCCTAA GACACCAATG 
GAGCGTTTCA AGAACACTTT CCTAAATCTG TCAATGCCAT TCTTCTCCTC TGCCGAACCA 
ATTGGCGCTC CAAAGAAAAC TTACATGGAC CGAGAGTTTA CTCTCTGGGA TAGAATTGAC 
GTTCAAGGAC CGCTCACACT TCAAGAATTC ATCGACAATG TACAAAACCA AACTGGTGGA 
TGCGAGGTGT CGATGTTATC CGCTGGAGCG TGTCTTTTGT TCTCGTTTTT CATGAATGCT 
GGCAAAAAAC AAGAAAGACT CAAAACAGAA GTGAAAGCGG TTTACGAGGA ATTGCTGAAG 
AAATCACTTC ATCCTTCTGT TCACGCTTTG GTTCTCGAGC CAATGATGTC CGATCCAGAC 
GGTGAAGATG TCGAAGTTCC ATACATTCGC TACTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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uba-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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