UBA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA1
Entrez Gene ID	282869
Full Name	ubiquitin like modifier activating enzyme 1
Synonyms	UBE1,UBE1X
General protein information	Preferred Names ubiquitin like modifier activating enzyme 1 Names ubiquitin-like modifier-activating enzyme 1 ubiquitin-activating enzyme E1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005228067.3	XP_005228124.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_005228068.3	XP_005228125.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_024987774.1	XP_024843542.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
NM_001102477.1	NP_001095947.1	ubiquitin-like modifier-activating enzyme 1
XM_005228068.2	XP_005228125.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_005228069.2	XP_005228126.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
XM_005228067.4	XP_005228124.1	ubiquitin-like modifier-activating enzyme 1 isoform X1

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Molecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses.
Oldham JM, Martyn JA, Sharma M, Jeanplong F, Kambadur R, Bass JJ
American journal of physiology. Regulatory, integrative and comparative physiology280(5)R1488-93(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa35511	NM_001102477.1 Latest version!	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35511	XM_024987774.1 Latest version!	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35511	XM_005228069.2 Latest version!	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35511	XM_005228067.4 Latest version!	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35511	XM_005228068.3 Latest version!	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa35511	XM_005228068.2	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35511	XM_005228067.3	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA1 ( NM_001102477.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095947.1 Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	NM_001102477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133293.1. On or before Aug 29, 2007 this sequence version replaced XM_864601.2, XM_001252171.1, XM_873786.2. ##Evidence-Data-START## Transcript exon combination :: BC133293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095947.1
CDS	29..3205
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_001102477.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102477.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_005228067.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228124.1 Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_005228067.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228124.1
CDS	98..3274
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005228067.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228067.3

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_005228068.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228125.1 Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_005228068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228125.1
CDS	108..3284
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005228068.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228068.3

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_024987774.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843542.1 Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_024987774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843542.1
CDS	314..3490
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_024987774.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987774.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_005228068.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228125.1 Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

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CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_005228068.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228125.1
CDS	153..3329
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005228068.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228068.2

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_005228069.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228126.1 Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_005228069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228126.1
CDS	319..3495
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005228069.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228069.2

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_005228067.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228124.1 Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35511
Clone ID Related Accession (Same CDS sequence)	XM_005228067.3 , XM_005228068.3 , XM_024987774.1 , NM_001102477.1 , XM_005228068.2 , XM_005228069.2 , XM_005228067.4
Accession Version	XM_005228067.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228067.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228124.1
CDS	130..3306
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_005228067.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228067.4

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGTT CCCCTGCCCA CTCCGTATTG TCCGAAGTGC CCTCGGTGCC AGCTAACGGA 
ATGGCGAAAA ATGTCAGTGA CGCAGACATA GATGAAGGCC TTTACTCCCG GCAGCTGTAT 
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACCTCCA GCGTACTGGT ATCAGGCCTT 
CGGGGCCTGG GCGTGGAGAT TGCCAAGAAC ATCATCCTCG GTGGGGTCAA GGCTGTCACA 
CTGCATGACC AGGGCACTGC CCAGTGGGCC GACCTCTCCT CCCAGTTCTA CCTGCGGGAA 
GAAGACATAG GAAAAAACCG AGCTGAGGTC TCCCAGCCCC GTCTCGCTGA GCTCAACAGC 
TATGTGCCTG TCAGTGCCTA CACCGGGCCC CTTGTGGAGG ACTTTCTCAG TGACTTCCAG 
GTGGTGGTCC TCACTAACAG CCCCCTGGAG GACCAGCTGC GGGTAGGTGA GTTCTGTCAC 
AGCCATGGCA TCAAGCTGGT GGTGGCAGAC ACCCGGGGCC TGTTCGGGCA GCTCTTCTGT 
GATTTTGGAG AGGAAATGAT CCTCACTGAT TCCAATGGGG AGCAGCCGCT CAGTGCTATG 
GTTTCTATGG TCACCAAGGA CAACCCCGGT GTTGTTACTT GTCTGGATGA GGCCCGACAT 
GGCTTTGAGA GTGGTGACTT CGTCTCCTTT TCGGAAGTAC AGGGCATGAT TGAACTCAAT 
GGAAGTCAGC CCATGGAAAT CAAAGTTCTG GGTCCTTACA CCTTTAGCAT CTGTGACACC 
TCCAACTTCT CTGACTACAT CCGCGGAGGC ATCGTCAGCC AGGTCAAAGT ACCCAAGAAG 
ATCAGCTTTA AATCCTTGCC AGCCTCCCTA GCAGAGCCTG ACTTTGTGAT GACAGACTTC 
GCCAAGTATT CCCGCCCTGC CCAGCTACAC ATTGGCTTCC AGGCCCTGCA CCATTTCTGT 
GCTCAGCATG GCCGGTCCCC TCGGCCCCAC AATGAGGAGG ATGCAGCAGA GCTGGTGACC 
ATAGCCCAGG CTGTGAACGC TCGATCCCTG CCCGCAGTGC AGCAGGGCAG CCTGGATGAG 
GACCTCATCC GGAAGCTAGC ATATGTGGCT GCTGGGGACC TGGCTCCCAT AAATGCCTTC 
ATTGGGGGCC TTGCTGCCCA GGAGGTCATG AAGGCTTGCT CTGGGAAGTT TATGCCCATC 
ATGCAGTGGC TGTACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGCCCTCACA 
GAGGACAAGT GCCTCCCGCG CCAGAACCGT TATGATGGGC AGGTAGCTGT ATTTGGCTCA 
GACCTGCAGG AGAGGTTGGG CAAGCAGAAG TACTTCCTGG TGGGCGCAGG GGCCATTGGC 
TGTGAACTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GTGCAGAAGA TGGAGAAATT 
GTTGTCACAG ACATGGACAC CATTGAGAAG TCAAATTTGA ACAGACAGTT TCTGTTCCGG 
CCCTGGGATG TCACTAAATT AAAGTCTGAC ACAGCTGCTG CAGCTGTGCG CCAGATGAAT 
CCTCATATCC GAGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA CCTGGATGGT GTAACCAATG CCCTGGATAA TGTGGATGCC 
CGCATGTACA TGGACCGCCG CTGCGTGTAC TACCGTAAGC CACTGCTGGA GTCGGGCACA 
CTGGGCACCA AGGGCAACGT GCAGGTGGTG ATCCCCTTCC TAACAGAATC TTACAGCTCC 
AGCCAGGACC CGCCTGAAAA GTCCATCCCC ATCTGCACAC TGAAGAACTT CCCCAATGCC 
ATCGAGCACA CCCTGCAGTG GGCTAGAGAT GAGTTCGAAG GCCTCTTCAA GCAGCCGGCA 
GAAAACGTCA ACCAATACCT CACAGACCCC AAGTTTGTGG AGCGGACCTT GCGACTGGCG 
GGCACTCAAC CCCTGGAGGT GCTGGAGGCC GTGCAGCGTA GTCTCGTGCT GCAGCGGCCA 
CAGACCTGGG CTGACTGTGT GACCTGGGCC TGCCACCACT GGCACACGCA GTACTCTAAC 
AATATCCGGC AGCTGCTACA CAACTTCCCT CCTGATCAGC TCACAAGCTC AGGAGCTCCA 
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCACTCACCT TTGATGTCAG CAATCCCCTC 
CATCTGGACT ACGTGATAGC TGCTGCCAAC CTGTTCGCCC AGACCTACGG GCTGACAGGC 
TCTCAGGACC GAGCTGCTGT GGCCACACTC CTACAGTCTG TGCAGGTCCC CGAGTTCACC 
CCCAAGTCTG GAGTCAAGAT CCATGTCTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT 
GTGGATGACA GCCGTCTGGA AGAGCTCAAG GCTACACTGC CCAGTCCCGA AAAGCTCCCT 
GGGTTCAAGA TGTACCCTAT CGACTTTGAG AAGGATGATG ACACCAACTT CCATATGGAT 
TTCATCGTGG CCGCATCCAA CCTCCGGGCA GAGAACTATG ACATTCCCCC TGCAGACCGG 
CACAAGAGCA AGCTGATTGC AGGAAAGATC ATCCCAGCCA TTGCCACAAC CACAGCAGCC 
GTGGTTGGCC TTGTGTGTTT AGAGCTGTAC AAGGTGGTGC AGGGGCACCG ACAGCTCAAC 
TCCTACAAGA ATGGTTTCCT TAACTTGGCC CTGCCCTTTT TTGGCTTCTC CGAGCCCCTC 
GCAGCACCTC GTCACCAGTA CTATAACCAA GAGTGGACGT TGTGGGATCG CTTTGAGGTA 
CAGGGTTTGC AGCCTAACGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTACTTCAAG 
ACAGAACATA AGTTGGAGAT CACTATGCTG TCCCAGGGCG TGTCTATGCT CTACTCTTTC 
TTCATGCCAG CCGCCAAGCT CAAGGAACGA TTGGATCAGC CGATGACCGA GATTGTAAGC 
CGTGTGTCGA AACGAAAGCT TGGCCGCCAT GTGCGAGCAC TGGTGCTTGA GCTGTGCTGT 
AACGATGAGA GCGGCGAGGA TGTTGAGGTC CCCTATGTAC GATACACCAT CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Molecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses.
American journal of physiology. Regulatory, integrative and comparative physiology280(5)R1488-93(2001 May)
Oldham JM,Martyn JA,Sharma M,Jeanplong F,Kambadur R,Bass JJ

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UBA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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