Alp12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alp12
Entrez Gene ID	35218
Full Name	CG16771 gene product from transcript CG16771-RE
Synonyms	Dmel\CG16771
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37D7-37E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201920.2	NP_001188849.1	alkaline phosphatase 12, isoform C
NM_001273647.1	NP_001260576.1	alkaline phosphatase 12, isoform E
NM_001201919.1	NP_001188848.1	alkaline phosphatase 12, isoform B
NM_001273646.2	NP_001260575.1	alkaline phosphatase 12, isoform D
NM_136119.4	NP_609963.1	alkaline phosphatase 12, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alp12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alp12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14961	NM_136119.4 Latest version!	Drosophila melanogaster alkaline phosphatase 12, transcript variant A (Alp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14961	NM_001201919.1 Latest version!	Drosophila melanogaster alkaline phosphatase 12, transcript variant B (Alp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14961	NM_001273646.2 Latest version!	Drosophila melanogaster alkaline phosphatase 12, transcript variant D (Alp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14961	NM_001273647.1 Latest version!	Drosophila melanogaster alkaline phosphatase 12, transcript variant E (Alp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14961	NM_001201920.2 Latest version!	Drosophila melanogaster alkaline phosphatase 12, transcript variant C (Alp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alp12 ( NM_001201920.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188849.1 Drosophila melanogaster alkaline phosphatase 12, transcript variant C (Alp12), mRNA.

Price & Availability↑

CloneID	OFa14961
Clone ID Related Accession (Same CDS sequence)	NM_001201920.2 , NM_001273647.1 , NM_001201919.1 , NM_001273646.2 , NM_136119.4
Accession Version	NM_001201920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alkaline phosphatase 12, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201920.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188849.1
CDS	203..1993
Translation	MEKRSGTPINSQKFRKLSDVDMTEVQLNNLDDSSAKETVEIGSFQGNDQSNPVQHTKWFRSRLFLISVVFSALLITAMCIGFVVHYGMSGDVGDMETVSYWPVDLPKEQKEWYDQGIDELQKAVSRQFNRRRAKNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATALFGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPAALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGSAADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANGHLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSEVLELNAAVESTLSFLKSSDRLDETLVIVTADHSHSLTINGHPDRGSSILGLAGNSKTEGTPYTTLTYGTSYQGFQVDPQTQSRKDPTADDITAWEYTQQAAINTDENLHGGSDVTIHADGAMSYLFHGVHEQSYVAHAISYALRIGRFRDSSIAETLAELTPI

Target ORF information:

RefSeq Version	NM_001201920.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alkaline phosphatase 12, transcript variant C (Alp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201920.2

ORF Insert Sequence:

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alp12 ( NM_001273647.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260576.1 Drosophila melanogaster alkaline phosphatase 12, transcript variant E (Alp12), mRNA.

Price & Availability↑

CloneID	OFa14961
Clone ID Related Accession (Same CDS sequence)	NM_001201920.2 , NM_001273647.1 , NM_001201919.1 , NM_001273646.2 , NM_136119.4
Accession Version	NM_001273647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alkaline phosphatase 12, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260576.1
CDS	744..2534
Translation	MEKRSGTPINSQKFRKLSDVDMTEVQLNNLDDSSAKETVEIGSFQGNDQSNPVQHTKWFRSRLFLISVVFSALLITAMCIGFVVHYGMSGDVGDMETVSYWPVDLPKEQKEWYDQGIDELQKAVSRQFNRRRAKNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATALFGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPAALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGSAADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANGHLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSEVLELNAAVESTLSFLKSSDRLDETLVIVTADHSHSLTINGHPDRGSSILGLAGNSKTEGTPYTTLTYGTSYQGFQVDPQTQSRKDPTADDITAWEYTQQAAINTDENLHGGSDVTIHADGAMSYLFHGVHEQSYVAHAISYALRIGRFRDSSIAETLAELTPI

Target ORF information:

RefSeq Version	NM_001273647.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alkaline phosphatase 12, transcript variant E (Alp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273647.1

ORF Insert Sequence:

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alp12 ( NM_001201919.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188848.1 Drosophila melanogaster alkaline phosphatase 12, transcript variant B (Alp12), mRNA.

Price & Availability↑

CloneID	OFa14961
Clone ID Related Accession (Same CDS sequence)	NM_001201920.2 , NM_001273647.1 , NM_001201919.1 , NM_001273646.2 , NM_136119.4
Accession Version	NM_001201919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alkaline phosphatase 12, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188848.1
CDS	74..1864
Translation	MEKRSGTPINSQKFRKLSDVDMTEVQLNNLDDSSAKETVEIGSFQGNDQSNPVQHTKWFRSRLFLISVVFSALLITAMCIGFVVHYGMSGDVGDMETVSYWPVDLPKEQKEWYDQGIDELQKAVSRQFNRRRAKNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATALFGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPAALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGSAADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANGHLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSEVLELNAAVESTLSFLKSSDRLDETLVIVTADHSHSLTINGHPDRGSSILGLAGNSKTEGTPYTTLTYGTSYQGFQVDPQTQSRKDPTADDITAWEYTQQAAINTDENLHGGSDVTIHADGAMSYLFHGVHEQSYVAHAISYALRIGRFRDSSIAETLAELTPI

Target ORF information:

RefSeq Version	NM_001201919.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alkaline phosphatase 12, transcript variant B (Alp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201919.1

ORF Insert Sequence:

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alp12 ( NM_001273646.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260575.1 Drosophila melanogaster alkaline phosphatase 12, transcript variant D (Alp12), mRNA.

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CloneID	OFa14961
Clone ID Related Accession (Same CDS sequence)	NM_001201920.2 , NM_001273647.1 , NM_001201919.1 , NM_001273646.2 , NM_136119.4
Accession Version	NM_001273646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alkaline phosphatase 12, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273646.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260575.1
CDS	441..2231
Translation	MEKRSGTPINSQKFRKLSDVDMTEVQLNNLDDSSAKETVEIGSFQGNDQSNPVQHTKWFRSRLFLISVVFSALLITAMCIGFVVHYGMSGDVGDMETVSYWPVDLPKEQKEWYDQGIDELQKAVSRQFNRRRAKNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATALFGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPAALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGSAADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANGHLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSEVLELNAAVESTLSFLKSSDRLDETLVIVTADHSHSLTINGHPDRGSSILGLAGNSKTEGTPYTTLTYGTSYQGFQVDPQTQSRKDPTADDITAWEYTQQAAINTDENLHGGSDVTIHADGAMSYLFHGVHEQSYVAHAISYALRIGRFRDSSIAETLAELTPI

Target ORF information:

RefSeq Version	NM_001273646.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alkaline phosphatase 12, transcript variant D (Alp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273646.2

ORF Insert Sequence:

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alp12 ( NM_136119.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609963.1 Drosophila melanogaster alkaline phosphatase 12, transcript variant A (Alp12), mRNA.

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CloneID	OFa14961
Clone ID Related Accession (Same CDS sequence)	NM_001201920.2 , NM_001273647.1 , NM_001201919.1 , NM_001273646.2 , NM_136119.4
Accession Version	NM_136119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alkaline phosphatase 12, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136119.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609963.1
CDS	479..2269
Translation	MEKRSGTPINSQKFRKLSDVDMTEVQLNNLDDSSAKETVEIGSFQGNDQSNPVQHTKWFRSRLFLISVVFSALLITAMCIGFVVHYGMSGDVGDMETVSYWPVDLPKEQKEWYDQGIDELQKAVSRQFNRRRAKNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATALFGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPAALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGSAADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANGHLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSEVLELNAAVESTLSFLKSSDRLDETLVIVTADHSHSLTINGHPDRGSSILGLAGNSKTEGTPYTTLTYGTSYQGFQVDPQTQSRKDPTADDITAWEYTQQAAINTDENLHGGSDVTIHADGAMSYLFHGVHEQSYVAHAISYALRIGRFRDSSIAETLAELTPI

Target ORF information:

RefSeq Version	NM_136119.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alkaline phosphatase 12, transcript variant A (Alp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136119.4

ORF Insert Sequence:

ATGGAAAAGC GAAGTGGAAC GCCGATAAAT AGCCAAAAGT TTCGAAAACT CAGCGATGTG 
GACATGACGG AAGTGCAGCT GAACAATTTG GATGACTCCA GTGCCAAGGA GACGGTGGAA 
ATAGGTAGCT TTCAGGGCAA TGACCAGAGC AATCCCGTGC AGCACACGAA ATGGTTCAGA 
TCCAGGCTCT TCCTCATATC CGTGGTTTTC TCGGCCCTCC TGATAACCGC CATGTGCATT 
GGCTTCGTTG TGCACTACGG CATGTCCGGC GATGTGGGCG ACATGGAGAC GGTGAGCTAC 
TGGCCCGTAG ATCTGCCCAA GGAGCAAAAG GAGTGGTACG ATCAGGGAAT CGATGAGCTT 
CAGAAGGCCG TTTCCAGGCA GTTCAATCGC CGGCGAGCCA AGAACGTAAT CCTGTTCGTG 
GGCGATGGAA TGGGTCCGAA CACGGTGACG GCCGCACGGA TACTCGGAGT CAAGGAGGAG 
GGACTCCTGC GTTGGGAGCA GTTTCCGGAC ATGGGACTGC TGAAGACCTA CTGTGCCGAC 
AAGCAAGTAC CGGACTCGTT CTCCACGGCC ACAGCTCTCT TTGGCGGAGT AAAGGTAAAC 
TACGAAACGG GAGGAGTTGA TGCAAATGTA CCGCTGGGGA ACTGCAGCGC ATCCCTGAAG 
GAGGACCACC ACGTTCAGAC GATCCTCAAG TGGGCCCAGG TGGATGGAAT GCGAACGGGT 
TTCGTGACCA CCACCCGGGT GACGCATGCC ACTCCGGCTG CCCTCTACGC CCACGTGCCG 
GATCGTCGAT GGGAGTGTGA GAGCGGAATG CCAGCCGAGG CTCAGGGTCA GGGATGTATG 
GACATAGCTC GCCAGCTGAT CGAACAGCCG ACGGGTCAGA GCATAAACGT AATCATGGGC 
GGTGGTCGTC AGATGCTGGT CTCAGATGTA ACCGGTTCAG CAGCCGATCC CCTGGACACT 
TGGGCCGGCC AGTCGAAGGA TGGACGAGAT CTCATCCGAG ATTGGAGGCT AAAGAAAGCG 
GATGAGGGAG TGTCACATGC GGTTGTGCAA AACAATCGTG AGCTGTGGAA TCTCAATGGT 
CAGGATGTTG ACTACGTACT TGGTATTTTC GCCAATGGAC ACCTAATGTA CGACCACGAA 
CGAGATCGCA GTGACGCCGG CATGCCCTCG CTGTCCAATA TGACCCTCAA GGCGCTGGAG 
GTCCTGGGAA ACAGTGATAA GGGGTTTCTG CTCGTGGTGG AAGCCGGTCT AATCGACCAA 
GCCCATCACA GGGGAAACGC TCGAAAGGCG CTAAGTGAGG TGCTCGAACT GAACGCAGCC 
GTTGAGTCTA CACTTTCCTT CCTCAAGTCA TCGGATCGCC TGGACGAAAC CTTGGTCATT 
GTGACCGCCG ATCACTCGCA CAGCCTGACC ATCAATGGGC ATCCCGATCG GGGATCCAGT 
ATACTCGGCT TGGCGGGAAA CTCGAAGACA GAAGGGACGC CGTACACCAC GTTAACCTAT 
GGCACCAGCT ATCAGGGATT CCAGGTGGAT CCGCAAACGC AGAGCCGCAA GGATCCAACT 
GCTGACGATA TTACCGCCTG GGAGTACACC CAGCAGGCAG CAATCAACAC GGACGAGAAT 
CTGCACGGTG GCTCGGATGT AACGATTCAC GCGGATGGTG CCATGTCCTA CTTGTTCCAC 
GGCGTCCACG AGCAGAGCTA TGTGGCGCAC GCCATATCGT ACGCCCTGAG GATCGGAAGA 
TTCCGGGATA GCTCCATCGC GGAAACTCTG GCCGAGCTGA CGCCCATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Alp12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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