Mhc cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Mhc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mhc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa41349 NM_001316398.1
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant V (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OFa14777 NM_165191.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant L (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14778 NM_165181.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant C (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14779 NM_165182.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant G (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14779 NM_001169520.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant O (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14780 NM_165183.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant E (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14781 NM_165185.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant F (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14782 NM_165186.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant D (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14783 NM_165187.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant A (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14784 NM_165188.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant I (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14785 NM_078863.6
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant H (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14786 NM_165190.4
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant K (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14787 NM_165192.4
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant M (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14788 NM_165189.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant B (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa14789 NM_165184.3
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant Q (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16842 NM_001259119.2
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant R (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16843 NM_001169519.2
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant N (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16844 NM_001169521.2
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant P (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16845 NM_001259122.1
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant U (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16846 NM_001259120.1
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant S (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa16847 NM_001259121.2
Latest version!
Drosophila melanogaster myosin heavy chain, transcript variant T (Mhc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa41349
    Clone ID Related Accession (Same CDS sequence) NM_001316398.1
    Accession Version NM_001316398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform V
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGGTA
    TTTCTGGGCC AACATATTGG TGATTATCCC GGCATTTGCC AGGGCAAAAC ACGGATACCC
    GGTGTCAACG ATGGGGAAGA ATTTGAGTTA ACTGACCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303327.1
    CDS276..6164
    Translation

    Target ORF information:

    RefSeq Version NM_001316398.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant V (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001316398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGGTA
    TTTCTGGGCC AACATATTGG TGATTATCCC GGCATTTGCC AGGGCAAAAC ACGGATACCC
    GGTGTCAACG ATGGGGAAGA ATTTGAGTTA ACTGACCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14777
    Clone ID Related Accession (Same CDS sequence) NM_165191.3
    Accession Version NM_165191.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165191.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC
    AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC
    AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724009.1
    CDS121..5931
    Translation

    Target ORF information:

    RefSeq Version NM_165191.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant L (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165191.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC
    AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC
    AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14778
    Clone ID Related Accession (Same CDS sequence) NM_165181.3
    Accession Version NM_165181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165181.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723999.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165181.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant C (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14779
    Clone ID Related Accession (Same CDS sequence) NM_165182.3 , NM_001169520.3
    Accession Version NM_165182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165182.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724000.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165182.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant G (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14779
    Clone ID Related Accession (Same CDS sequence) NM_165182.3 , NM_001169520.3
    Accession Version NM_001169520.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169520.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162991.1
    CDS283..6171
    Translation

    Target ORF information:

    RefSeq Version NM_001169520.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant O (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169520.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14780
    Clone ID Related Accession (Same CDS sequence) NM_165183.3
    Accession Version NM_165183.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165183.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724001.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165183.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant E (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14781
    Clone ID Related Accession (Same CDS sequence) NM_165185.3
    Accession Version NM_165185.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165185.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGGTACC AGATCCTGAA CCCCCGTGGC ATTAAGGACC TCGATTGTCC CAAGAAAGCC
    TCCAAGGTTC TGATTGAGTC CACCGAGCTG AACGAAGACC TTTACCGTCT GGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724003.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165185.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant F (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGGTACC AGATCCTGAA CCCCCGTGGC ATTAAGGACC TCGATTGTCC CAAGAAAGCC
    TCCAAGGTTC TGATTGAGTC CACCGAGCTG AACGAAGACC TTTACCGTCT GGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14782
    Clone ID Related Accession (Same CDS sequence) NM_165186.3
    Accession Version NM_165186.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165186.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724004.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165186.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant D (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165186.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14783
    Clone ID Related Accession (Same CDS sequence) NM_165187.3
    Accession Version NM_165187.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165187.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724005.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165187.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant A (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165187.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14784
    Clone ID Related Accession (Same CDS sequence) NM_165188.3
    Accession Version NM_165188.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165188.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724006.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165188.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant I (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165188.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14785
    Clone ID Related Accession (Same CDS sequence) NM_078863.6
    Accession Version NM_078863.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078863.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523587.4
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_078863.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant H (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078863.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT
    AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC
    CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT
    GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14786
    Clone ID Related Accession (Same CDS sequence) NM_165190.4
    Accession Version NM_165190.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165190.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC
    AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC
    AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT
    GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724008.1
    CDS289..6099
    Translation

    Target ORF information:

    RefSeq Version NM_165190.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant K (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165190.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC
    AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC
    AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT
    GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14787
    Clone ID Related Accession (Same CDS sequence) NM_165192.4
    Accession Version NM_165192.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165192.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724010.1
    CDS121..5931
    Translation

    Target ORF information:

    RefSeq Version NM_165192.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant M (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165192.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14788
    Clone ID Related Accession (Same CDS sequence) NM_165189.3
    Accession Version NM_165189.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724007.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_165189.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant B (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14789
    Clone ID Related Accession (Same CDS sequence) NM_165184.3
    Accession Version NM_165184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform Q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_165184.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724002.2
    CDS263..6151
    Translation

    Target ORF information:

    RefSeq Version NM_165184.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant Q (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16842
    Clone ID Related Accession (Same CDS sequence) NM_001259119.2
    Accession Version NM_001259119.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform R
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259119.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246048.1
    CDS121..6009
    Translation

    Target ORF information:

    RefSeq Version NM_001259119.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant R (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259119.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16843
    Clone ID Related Accession (Same CDS sequence) NM_001169519.2
    Accession Version NM_001169519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169519.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162990.1
    CDS121..5970
    Translation

    Target ORF information:

    RefSeq Version NM_001169519.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant N (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16844
    Clone ID Related Accession (Same CDS sequence) NM_001169521.2
    Accession Version NM_001169521.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169521.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162992.1
    CDS121..5970
    Translation

    Target ORF information:

    RefSeq Version NM_001169521.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant P (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169521.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC
    TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC
    AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT
    GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16845
    Clone ID Related Accession (Same CDS sequence) NM_001259122.1
    Accession Version NM_001259122.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform U
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246051.1
    CDS281..6130
    Translation

    Target ORF information:

    RefSeq Version NM_001259122.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant U (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259122.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT
    CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC
    AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC
    ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG
    GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16846
    Clone ID Related Accession (Same CDS sequence) NM_001259120.1
    Accession Version NM_001259120.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246049.1
    CDS276..6164
    Translation

    Target ORF information:

    RefSeq Version NM_001259120.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant S (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259120.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC
    AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT
    TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC
    ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC
    CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16847
    Clone ID Related Accession (Same CDS sequence) NM_001259121.2
    Accession Version NM_001259121.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product myosin heavy chain, isoform T
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259121.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT
    GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246050.1
    CDS71..5959
    Translation

    Target ORF information:

    RefSeq Version NM_001259121.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster myosin heavy chain, transcript variant T (Mhc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259121.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
    TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC
    CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC
    TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG
    AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC
    TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT
    CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT
    AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC
    GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG
    TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT
    GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG
    CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC
    TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT
    GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT
    TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT
    CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC
    AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC
    ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC
    ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT
    GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG
    CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC
    AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG
    AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT
    ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC
    AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC
    AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC
    GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC
    AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT
    CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC
    CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT
    CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC
    TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG
    AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG
    ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG
    CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT
    GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG
    ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT
    GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC
    AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG
    GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC
    AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC
    CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC
    GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA
    CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG
    GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC
    CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT
    ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG
    AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC
    CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG
    ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG
    ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC
    AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG
    AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC
    ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG
    CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT
    CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG
    GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC
    GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT
    GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC
    AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC
    GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG
    AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG
    AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG
    AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC
    ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG
    TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG
    GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC
    AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG
    AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC
    CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG
    GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG
    TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT
    GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC
    GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG
    TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG
    AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC
    AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG
    GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT
    CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG
    GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC
    GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC
    ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG
    AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG
    CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC
    CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG
    GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC
    TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG
    GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT
    GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA
    TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG
    GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG
    CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC
    AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC
    GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG
    ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG
    GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC
    GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC
    GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC
    GAATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.