Smg5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Smg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa16743 NM_165069.2
Latest version!
Drosophila melanogaster Smg5, transcript variant B (Smg5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16743 NM_001298985.1
Latest version!
Drosophila melanogaster Smg5, transcript variant C (Smg5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16743 NM_135841.5
Latest version!
Drosophila melanogaster Smg5, transcript variant A (Smg5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa16743
    Clone ID Related Accession (Same CDS sequence) NM_165069.2 , NM_135841.5 , NM_001298985.1
    Accession Version NM_165069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Smg5, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165069.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723851.1
    CDS282..3815
    Translation

    Target ORF information:

    RefSeq Version NM_165069.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Smg5, transcript variant B (Smg5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16743
    Clone ID Related Accession (Same CDS sequence) NM_165069.2 , NM_135841.5 , NM_001298985.1
    Accession Version NM_135841.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Smg5, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135841.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_609685.1
    CDS276..3809
    Translation

    Target ORF information:

    RefSeq Version NM_135841.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Smg5, transcript variant A (Smg5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_135841.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16743
    Clone ID Related Accession (Same CDS sequence) NM_165069.2 , NM_135841.5 , NM_001298985.1
    Accession Version NM_001298985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Smg5, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285914.1
    CDS116..3649
    Translation

    Target ORF information:

    RefSeq Version NM_001298985.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Smg5, transcript variant C (Smg5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC 
    GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
    GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
    AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
    CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
    CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
    CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTC
    CTCTGCGCTG GCATCTTCAA CTACAAACGA TTGTCGGCAC GCATGGAAGA GATCTATGAT
    CTGGATCTAA AGTATTTGAT TGATTTCTCC ATAATCAGTG ATGGCTACAT AAGCGACATG
    ATCGGAGACA CCATGGAGTC ATCGCACTCG GGCACCCACG CGGTGGACAA GAGTCTGGAG
    GCCGTGTCCT TTGCACTCGA CACCATACAC TCATCGCTGC TCAGTCTGGG CGATCTTCAT
    CGCTACTTTC TAGACTTTCG CATCGACAGC AAACTAAGCA TAAGCAAGGA GCAGGTGGCC
    AAGTATTATC TGGAGGCCTT CAAGCTTAAT CCGGCCATTG GCATGGCCCA GAACCAGTTG
    GGTACACTGC ACTACGGACA GAATCATGAC CTGGACTCCA CTTACCACTA CTTGTACTCC
    CTTGTGTGCA TCATACCATT CGAACTAAGC GAAAATAATC TTAACAAGCT GTTCGCCAAG
    CACACCGAAT ACCTAGAGCG TATGGATCCA GAAAAACTAG ACTTCAACAT CGAGGACTTC
    TTTGCCCGTT TCTATCTGAT TGTGGACATA TTTTTCTTCG ACAAGGAAGT GCCGGACTTT
    AACGCTCTGT GCCACTGTGT GCTGATTGAT CTACGCAAGA TCCTGTGCTC CCAGCAGCTG
    CGCGTTCCGG AGCCAAGCAT CTTCAAGATT GTCTCCATTC TGTTCTTCTG TCTGTCCAAG
    CTTAAGATGA TCAACTCACC AAAGGTTTAC TCGCTGCACG CCTTTCTGGT TGCTGCCTGT
    TCCGATCTGA TGGACGCCTG CATCGTCAGC ATCGAGCAGA CAATCCTGGC TCGCGCCCAA
    GACAACGAGC GCTTCCAGGC GGAGTACGAG GAACGCTTTC AGGAGTTCGA TACTGTGGTG
    CGCAAGTCCC GGAATGAGTA CAAGAACTGG CTAGCTGCAG TAGGCGAATG TGAGCGCAAG
    GACAAGAAGT TGATGCCACG CCCGCACTCT CTAAAGGATT GCAGTTCGGA TTCGCGGGAC
    AGCCAGCACT CTGGCGAGGA GGCAAAGACC CAGGAGGCTG CTGACGGCGA CAAGATCGGT
    GAGGCCGTTT CCACGCGCTC CAGTGACGAG GCCAAGGAGT CCGACCTTAA GACCCCGCTC
    TCCTGCAAGA GCAAGCGACG GGGAATGCGT CTACGCCGCC GACGCCGCAA GATCGCCCAG
    TCGGACGACA GCTCCTGCTA TGACAGCGAA AGCGAACTGG ACACAGACTT TTACAGCGAC
    GAGGAGGATT ACACAGATCT AAGTTCTAAC TTTGACACCG ATTTCAGCGA CATTGATGCA
    GCTGATCCTG GGGAGCCATG CGGCGAAGAG CGCTATGCAG CAGCTAATTA CAGCAAAAAG
    GAGCGCAAAG CTGGCGGCGG CGGCGGAGGA GGAGTCGGTG GCGTGGTGTA CTCCGACAAC
    GAAGACGTTG TTATTGAGGA GGAGAAAATC GTGTATCCCA ACGAAGTAGA GCGCAGATCC
    AACTCACAGG AGGCGGACTC TGAGGAGTTG CTGCGCAGCT TTGAGGTACT GCAGCTGAAC
    TTTAAGAGCG CAGAGGATGC TCAAAGCAAG CCGGTGGCAC CTCCGCCCGT CAGCTTTTCA
    GAGCCACCAA AGAAGCTGGT TTATCGCAAC AAGTACACCA AGATCAATCC CAACATAGTC
    ATTGACTGTC TGCACAATGA ACCGACCATT AGAGCCCTGA AAATGCTTTT TGACTGGCTG
    CGAATCAACC CAGAGATTCT CATCGGCTGC TACCACTCGA ATCCGGAGTT TGTGCACAAG
    ATCATGAAGC TTCTCAACTA CTGCAACATC GACATCTTCA CCAGGAAGGT TTACTTCGAG
    CGCGACCTTC TGACCACTGC CAACGTGCGT GATTCTCTGG TCGAGTTATT CGATGTGCGC
    GCCAACGTTC CGCTCACCGA GGACTTTGCT TTCAAGAACT TTGACATCTT CCAGAGCACC
    CAGATTACAC TGGACTGGGA GACCATTATC CGGGAGCGTG TCACTCCCCA GGAAGAGTCC
    ATTCTCCGTA TTTTCAAAAT GGTCGACTTT GGCTTTTTCA TTTGCAAGCA GAAGAAGTTT
    AACTACAGAT TCTGCGTCAA GACGCGTCGT TTCACCGAGA CCCAAAAGAA GAAACAGCGC
    GAGGAGAAGA AGGGAGGCGG ATCACGTCGC CGCGAGCGTC GTACCGCTCC GAAGACCAAC
    CGAACAAAGC GGAACGAAGG ACGCACACGT CGCTACTCCG ATCGCAAGAA CGGCATCGTC
    CGCAAGAGCT ACACCAGTAA CGAGGAAAAG GACACCGTGG AGGAGTGCCG CAAGATGCCA
    CGCAAGGGTT ACCTAAGGAA TCGCAATGCC GAGAAGGCGG CTGCTGTTAT AACCAGTGCC
    ACGGGAACCT CTGTCGCCGG CGGAACCTCC GCCTCCACAA CCACAAACAG TGTCATCGAG
    AAACTGAATT TGCAGTCCAG CTCGGGGGCC GACGAAGAGC GCTCGGAGGC CAAGTCGAAG
    AAGTGTGAGC TCATGGGCAA GCTGTGGCTG CAAAACGAGG TGGAGACTTT GGAGGAGGAG
    CTGCGGGACA GTCCCGCTCA TGTGGTGATG TCCCCATTTG TGGTGGTCGA TTCGAAGGCA
    TTGACCGAAT ACAATGGCAT AGTCAAGTCG CTGGTGAGGA CCAAGAAGTG CATTGTCCTC
    ATTCCAAATG CAGTGCTCAA TGAGTTGGAT GACTTGAAGA AACGCAGCGA GAATGTGCGC
    CATGTCATCC GTTGGCTGGA GCAGGAGTTT AAACAGGGCA ATAGGCACTT GCGTGCCCAG
    CGCGATAACG AAAGTCAGCC GCTATCCCTG CTGAAGATAT CACGCAAGAT GGATCGTGAC
    GCCTCAGTTT TCCTTCAAAT TGCCCAGTTC TGCAATCACT TGGTGGCCAA CCATGCCGAT
    CCGCACGTCA GCGAACTGCA GAAGAGCGTC GTCACGTACT TGTCCGGCGA TAATTTGCAC
    GAGAAGAACC GCCAGCAACA GAACTTCAGC TACACCGGCA TCCTTGACTC GATACCCGTA
    CGCTATGCCC AAATCCAGAG CTTCTACGCA AAATTTAAGG CCAACAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.