nub cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following nub gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nub cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa14549 NM_001298947.1
Latest version!
Drosophila melanogaster nubbin (nub), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa14549 NM_057311.5
Latest version!
Drosophila melanogaster nubbin (nub), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa14548 NM_001103683.2
Latest version!
Drosophila melanogaster nubbin (nub), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa14549
    Clone ID Related Accession (Same CDS sequence) NM_001298947.1 , NM_057311.5
    Accession Version NM_001298947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nubbin, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
    AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG
    CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285876.1
    CDS214..2019
    Translation

    Target ORF information:

    RefSeq Version NM_001298947.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nubbin (nub), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
    AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG
    CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14549
    Clone ID Related Accession (Same CDS sequence) NM_001298947.1 , NM_057311.5
    Accession Version NM_057311.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nubbin, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057311.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
    AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG
    CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476659.1
    CDS652..2457
    Translation

    Target ORF information:

    RefSeq Version NM_057311.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nubbin (nub), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057311.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
    AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG
    CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14548
    Clone ID Related Accession (Same CDS sequence) NM_001103683.2
    Accession Version NM_001103683.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nubbin, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103683.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAACGC CGACAACAAC ATCGAAATAC AAAATCAATG GCGAGGCCAC CGAAGGCGTT 
    TCAGCATCCA CACTGCTGTA TCATCATCCG CATCACAGCC ATCGTCATCA TCACCATCAG
    CAGTCGCAAC AGCAGCAGCT ACATCAGCAG CAGCAGCAGC AGCAACATCA TCATCAGCAG
    CAGCACCAGT TGCTGCTGCA GCAACAGCTC CTTCAGCAAC ATGTGGCCAA CAGTTCACCA
    CTGCAACATC TGAGCAATCT GTTTGGCCAA CATTTGCCTA CGCACAGCCT GCAGCACTTG
    ATTGCCGCCG AGTACTGGCA GCAGCAGCAG CAACAGCCAG CAGCAACAAT TAAAAGCGAG
    CCAACAACTC AATCAGTGGC AGCAACATCG GTCTTTAGCT TCACGCCGCA GCAACACGTC
    AATTTTGCCG CCTCATTAGT CGCATTACAG CAAAATGATG CCTTGCCCGA GGAAACAATG
    GCAACGGCAA CAGCAACACC GACACCGGCG ACAACTGCAT CAGGAGCGGC AACTGCAGCA
    GCAGTCGCAA CAAGCCCTGC AGCAGCGACA ACATTGAAGG CGCCAACAGC AACACAGGCG
    GCAGCAACAA TGGACGACAT CGATGATGAT GATGACGACA GCAACAACGG CGGCCACATG
    CAAAATGCAG GCGTTGGCAA CTTTAAATTC GAGCCAGGCC AACAACTTGG CACGCCGGCG
    AATGAAGCTG TCCTAACGAG TTCCGAGGAG AACAAAGATG GAACTTTGAC TGCTGTTGAC
    ACAAATATGC GCGATGTCAG CGCAAATCAC AGCAGCTCCT TTCTTTCGTC CTCGCCCGCC
    TCCTCGACTC TGGTGTCCTG TCACTCCCCC TCGCCGCCGC TGCCGCTGCT TGCGGACCAG
    GACGATGGAA CGAGGAGGAT GCGGGACAAG AAGCTAACGC TCGAATTGAA GTTGCAATCG
    TGTGATGAAT ATGTTGAGCA GCCAAAGGAT AAAGGAGCCG AACGGCTGCA ACCAGCTCAG
    ATTGCCAACG ACAATGCTCC GTCGCCTCTG CATTTGAAAC TGGAGGAGCA GGATGAGCAG
    GAGCAGGCGG ACGTAAAGGA TAAGGTCCTG GAACTGACCG GAGCAGCAGT TGCTCTCTAT
    GGCCACTTGA ACAATGCCAG CAAGGATGTG CGAGATTTAG AGCAGTGTCT GAAATTGCAG
    GACCCAAATG ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097153.1
    CDS802..3687
    Translation

    Target ORF information:

    RefSeq Version NM_001103683.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nubbin (nub), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103683.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAACGC CGACAACAAC ATCGAAATAC AAAATCAATG GCGAGGCCAC CGAAGGCGTT 
    TCAGCATCCA CACTGCTGTA TCATCATCCG CATCACAGCC ATCGTCATCA TCACCATCAG
    CAGTCGCAAC AGCAGCAGCT ACATCAGCAG CAGCAGCAGC AGCAACATCA TCATCAGCAG
    CAGCACCAGT TGCTGCTGCA GCAACAGCTC CTTCAGCAAC ATGTGGCCAA CAGTTCACCA
    CTGCAACATC TGAGCAATCT GTTTGGCCAA CATTTGCCTA CGCACAGCCT GCAGCACTTG
    ATTGCCGCCG AGTACTGGCA GCAGCAGCAG CAACAGCCAG CAGCAACAAT TAAAAGCGAG
    CCAACAACTC AATCAGTGGC AGCAACATCG GTCTTTAGCT TCACGCCGCA GCAACACGTC
    AATTTTGCCG CCTCATTAGT CGCATTACAG CAAAATGATG CCTTGCCCGA GGAAACAATG
    GCAACGGCAA CAGCAACACC GACACCGGCG ACAACTGCAT CAGGAGCGGC AACTGCAGCA
    GCAGTCGCAA CAAGCCCTGC AGCAGCGACA ACATTGAAGG CGCCAACAGC AACACAGGCG
    GCAGCAACAA TGGACGACAT CGATGATGAT GATGACGACA GCAACAACGG CGGCCACATG
    CAAAATGCAG GCGTTGGCAA CTTTAAATTC GAGCCAGGCC AACAACTTGG CACGCCGGCG
    AATGAAGCTG TCCTAACGAG TTCCGAGGAG AACAAAGATG GAACTTTGAC TGCTGTTGAC
    ACAAATATGC GCGATGTCAG CGCAAATCAC AGCAGCTCCT TTCTTTCGTC CTCGCCCGCC
    TCCTCGACTC TGGTGTCCTG TCACTCCCCC TCGCCGCCGC TGCCGCTGCT TGCGGACCAG
    GACGATGGAA CGAGGAGGAT GCGGGACAAG AAGCTAACGC TCGAATTGAA GTTGCAATCG
    TGTGATGAAT ATGTTGAGCA GCCAAAGGAT AAAGGAGCCG AACGGCTGCA ACCAGCTCAG
    ATTGCCAACG ACAATGCTCC GTCGCCTCTG CATTTGAAAC TGGAGGAGCA GGATGAGCAG
    GAGCAGGCGG ACGTAAAGGA TAAGGTCCTG GAACTGACCG GAGCAGCAGT TGCTCTCTAT
    GGCCACTTGA ACAATGCCAG CAAGGATGTG CGAGATTTAG AGCAGTGTCT GAAATTGCAG
    GACCCAAATG ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC
    GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG
    AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC
    CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC
    AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT
    AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT
    CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG
    GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG
    GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA
    CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG
    TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA
    CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC
    GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG
    GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG
    GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC
    TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT
    CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT
    GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG
    CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG
    GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA
    ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT
    GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG
    TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC
    ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT
    GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG
    GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG
    AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC
    ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.