nub cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nub
Entrez Gene ID	34669
Full Name	nubbin
Synonyms	CG15488,CG15489,CG34395,CG6246,Dmel\CG34395,Dmel_CG15488,Dmel_CG15489,Dmel_CG6246,NUB,Nb,Nub,Oct-1,Oct1,PDM-1,PDM1,POU33F1,Pdm,Pdm-1,Pdm1,dOct1,dPOU-19,dPOU-19/pdm-1,dPOU19,nb,pdm,pdm-1,pdm1,twn
General protein information	Preferred Names nubbin Names CG34395 gene product from transcript CG34395-RB CG34395-PB CG34395-PD CG34395-PE NUBBIN POU domain protein 1 nub-PB nub-PD nub-PE twain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33F1-33F1\|2-47.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298947.1	NP_001285876.1	nubbin, isoform E
NM_001103683.2	NP_001097153.1	nubbin, isoform B
NM_057311.5	NP_476659.1	nubbin, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009500	XP_310195.4
Drosophila melanogaster (fruit fly)	nub	NP_001097153.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that Pdm1 is necessary for a beneficial gut microbiota and normal lifespan
Title: The POU/Oct Transcription Factor Pdm1/nub Is Necessary for a Beneficial Gut Microbiota and Normal Lifespan of Drosophila.

We suggest that Nub protein plays an ancient role, shared with mammalian Oct/POU transcription factors, to moderate responses to immune challenge, thereby increasing the tolerance to biotic stress.
Title: The Oct1 homolog Nubbin is a repressor of NF-?B-dependent immune gene expression that increases the tolerance to gut microbiota.

Pdm-1 and Drosophila octamer elements dictate the coordinated transcription of core histone genes
Title: Drosophila octamer elements and Pdm-1 dictate the coordinated transcription of core histone genes.

These results provide a new neuroblast lineage for investigating temporal identity and reveal the importance of Pdm and Cas as timing factors that close temporal identity windows.
Title: Pdm and Castor close successive temporal identity windows in the NB3-1 lineage.

Nub and Tsh domains share some of the attributes of classical compartments, but lack their stringent and immobile boundaries.
Title: Nubbin and Teashirt mark barriers to clonal growth along the proximal-distal axis of the Drosophila wing.

The following nub gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nub cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14549	NM_001298947.1 Latest version!	Drosophila melanogaster nubbin (nub), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14549	NM_057311.5 Latest version!	Drosophila melanogaster nubbin (nub), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14548	NM_001103683.2 Latest version!	Drosophila melanogaster nubbin (nub), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nub ( NM_001298947.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285876.1 Drosophila melanogaster nubbin (nub), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa14549
Clone ID Related Accession (Same CDS sequence)	NM_001298947.1 , NM_057311.5
Accession Version	NM_001298947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nubbin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG 
CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285876.1
CDS	214..2019
Translation	MVMSELRWHTASPEDNKNSLKRDLLKSTPTSAREAAVHIMQNRYISRLSRSPSPLQSNASDCDDNNSSVGTSSDRCRSPLSPALSLSHQQAKRQLMSLQPHPAHHHHNPHHLNHLNHHQYKQEEDYDDANGGALNLTSDNSRHSTQSPSNSVKSATASPVPVISVPSPVPPMISPVLAPSGCGSTTPNSMAAAAAAAAAVASTMGSGISPLLALPGMSSPQAQLAAAGLGMNNPLLTGSLSPQDFAQFHQLLQQRQVALTQQFNSYMELLRSGSLGLAQDDPALTAQVAAAQFLMQSQLQALSQASQQLQALQKQQQRQVDEPLQLNHKMTQQPRSSTPHSIRSPIAIRSPASSPQQLHHHHHHPLQITPPSSAASLKLSGMLTPSTPTSGTQMSQGTTTPQPKTVASAAAARAAGEPSPEETTDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLQKWLDDADRTIQATGGVFDPAALQATVSTPEIIGRRRKKRTSIETTIRGALEKAFLANQKPTSEEITQLADRLSMEKEVVRVWFCNRRQKEKRINPSLDSPTGADDDESSYMMH

Target ORF information:

RefSeq Version	NM_001298947.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nubbin (nub), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298947.1

ORF Insert Sequence:

ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG 
CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nub ( NM_057311.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476659.1 Drosophila melanogaster nubbin (nub), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14549
Clone ID Related Accession (Same CDS sequence)	NM_001298947.1 , NM_057311.5
Accession Version	NM_057311.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nubbin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057311.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG 
CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476659.1
CDS	652..2457
Translation	MVMSELRWHTASPEDNKNSLKRDLLKSTPTSAREAAVHIMQNRYISRLSRSPSPLQSNASDCDDNNSSVGTSSDRCRSPLSPALSLSHQQAKRQLMSLQPHPAHHHHNPHHLNHLNHHQYKQEEDYDDANGGALNLTSDNSRHSTQSPSNSVKSATASPVPVISVPSPVPPMISPVLAPSGCGSTTPNSMAAAAAAAAAVASTMGSGISPLLALPGMSSPQAQLAAAGLGMNNPLLTGSLSPQDFAQFHQLLQQRQVALTQQFNSYMELLRSGSLGLAQDDPALTAQVAAAQFLMQSQLQALSQASQQLQALQKQQQRQVDEPLQLNHKMTQQPRSSTPHSIRSPIAIRSPASSPQQLHHHHHHPLQITPPSSAASLKLSGMLTPSTPTSGTQMSQGTTTPQPKTVASAAAARAAGEPSPEETTDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLQKWLDDADRTIQATGGVFDPAALQATVSTPEIIGRRRKKRTSIETTIRGALEKAFLANQKPTSEEITQLADRLSMEKEVVRVWFCNRRQKEKRINPSLDSPTGADDDESSYMMH

Target ORF information:

RefSeq Version	NM_057311.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nubbin (nub), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057311.5

ORF Insert Sequence:

ATGGTTATGT CGGAGCTACG TTGGCACACC GCTAGTCCCG AGGATAATAA GAATTCCTTG 
AAGCGCGATT TGCTCAAATC CACACCAACC AGTGCCCGCG AGGCCGCCGT TCACATAATG 
CAGAATCGAT ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nub ( NM_001103683.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097153.1 Drosophila melanogaster nubbin (nub), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14548
Clone ID Related Accession (Same CDS sequence)	NM_001103683.2
Accession Version	NM_001103683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nubbin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103683.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACGC CGACAACAAC ATCGAAATAC AAAATCAATG GCGAGGCCAC CGAAGGCGTT 
TCAGCATCCA CACTGCTGTA TCATCATCCG CATCACAGCC ATCGTCATCA TCACCATCAG 
CAGTCGCAAC AGCAGCAGCT ACATCAGCAG CAGCAGCAGC AGCAACATCA TCATCAGCAG 
CAGCACCAGT TGCTGCTGCA GCAACAGCTC CTTCAGCAAC ATGTGGCCAA CAGTTCACCA 
CTGCAACATC TGAGCAATCT GTTTGGCCAA CATTTGCCTA CGCACAGCCT GCAGCACTTG 
ATTGCCGCCG AGTACTGGCA GCAGCAGCAG CAACAGCCAG CAGCAACAAT TAAAAGCGAG 
CCAACAACTC AATCAGTGGC AGCAACATCG GTCTTTAGCT TCACGCCGCA GCAACACGTC 
AATTTTGCCG CCTCATTAGT CGCATTACAG CAAAATGATG CCTTGCCCGA GGAAACAATG 
GCAACGGCAA CAGCAACACC GACACCGGCG ACAACTGCAT CAGGAGCGGC AACTGCAGCA 
GCAGTCGCAA CAAGCCCTGC AGCAGCGACA ACATTGAAGG CGCCAACAGC AACACAGGCG 
GCAGCAACAA TGGACGACAT CGATGATGAT GATGACGACA GCAACAACGG CGGCCACATG 
CAAAATGCAG GCGTTGGCAA CTTTAAATTC GAGCCAGGCC AACAACTTGG CACGCCGGCG 
AATGAAGCTG TCCTAACGAG TTCCGAGGAG AACAAAGATG GAACTTTGAC TGCTGTTGAC 
ACAAATATGC GCGATGTCAG CGCAAATCAC AGCAGCTCCT TTCTTTCGTC CTCGCCCGCC 
TCCTCGACTC TGGTGTCCTG TCACTCCCCC TCGCCGCCGC TGCCGCTGCT TGCGGACCAG 
GACGATGGAA CGAGGAGGAT GCGGGACAAG AAGCTAACGC TCGAATTGAA GTTGCAATCG 
TGTGATGAAT ATGTTGAGCA GCCAAAGGAT AAAGGAGCCG AACGGCTGCA ACCAGCTCAG 
ATTGCCAACG ACAATGCTCC GTCGCCTCTG CATTTGAAAC TGGAGGAGCA GGATGAGCAG 
GAGCAGGCGG ACGTAAAGGA TAAGGTCCTG GAACTGACCG GAGCAGCAGT TGCTCTCTAT 
GGCCACTTGA ACAATGCCAG CAAGGATGTG CGAGATTTAG AGCAGTGTCT GAAATTGCAG 
GACCCAAATG ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097153.1
CDS	802..3687
Translation	MSTPTTTSKYKINGEATEGVSASTLLYHHPHHSHRHHHHQQSQQQQLHQQQQQQQHHHQQQHQLLLQQQLLQQHVANSSPLQHLSNLFGQHLPTHSLQHLIAAEYWQQQQQQPAATIKSEPTTQSVAATSVFSFTPQQHVNFAASLVALQQNDALPEETMATATATPTPATTASGAATAAAVATSPAAATTLKAPTATQAAATMDDIDDDDDDSNNGGHMQNAGVGNFKFEPGQQLGTPANEAVLTSSEENKDGTLTAVDTNMRDVSANHSSSFLSSSPASSTLVSCHSPSPPLPLLADQDDGTRRMRDKKLTLELKLQSCDEYVEQPKDKGAERLQPAQIANDNAPSPLHLKLEEQDEQEQADVKDKVLELTGAAVALYGHLNNASKDVRDLEQCLKLQDPNDISRLSRSPSPLQSNASDCDDNNSSVGTSSDRCRSPLSPALSLSHQQAKRQLMSLQPHPAHHHHNPHHLNHLNHHQYKQEEDYDDANGGALNLTSDNSRHSTQSPSNSVKSATASPVPVISVPSPVPPMISPVLAPSGCGSTTPNSMAAAAAAAAAVASTMGSGISPLLALPGMSSPQAQLAAAGLGMNNPLLTGSLSPQDFAQFHQLLQQRQVALTQQFNSYMELLRSGSLGLAQDDPALTAQVAAAQFLMQSQLQALSQASQQLQALQKQQQRQVDEPLQLNHKMTQQPRSSTPHSIRSPIAIRSPASSPQQLHHHHHHPLQITPPSSAASLKLSGMLTPSTPTSGTQMSQGTTTPQPKTVASAAAARAAGEPSPEETTDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLQKWLDDADRTIQATGGVFDPAALQATVSTPEIIGRRRKKRTSIETTIRGALEKAFLANQKPTSEEITQLADRLSMEKEVVRVWFCNRRQKEKRINPSLDSPTGADDDESSYMMH

Target ORF information:

RefSeq Version	NM_001103683.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nubbin (nub), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103683.2

ORF Insert Sequence:

ATGTCAACGC CGACAACAAC ATCGAAATAC AAAATCAATG GCGAGGCCAC CGAAGGCGTT 
TCAGCATCCA CACTGCTGTA TCATCATCCG CATCACAGCC ATCGTCATCA TCACCATCAG 
CAGTCGCAAC AGCAGCAGCT ACATCAGCAG CAGCAGCAGC AGCAACATCA TCATCAGCAG 
CAGCACCAGT TGCTGCTGCA GCAACAGCTC CTTCAGCAAC ATGTGGCCAA CAGTTCACCA 
CTGCAACATC TGAGCAATCT GTTTGGCCAA CATTTGCCTA CGCACAGCCT GCAGCACTTG 
ATTGCCGCCG AGTACTGGCA GCAGCAGCAG CAACAGCCAG CAGCAACAAT TAAAAGCGAG 
CCAACAACTC AATCAGTGGC AGCAACATCG GTCTTTAGCT TCACGCCGCA GCAACACGTC 
AATTTTGCCG CCTCATTAGT CGCATTACAG CAAAATGATG CCTTGCCCGA GGAAACAATG 
GCAACGGCAA CAGCAACACC GACACCGGCG ACAACTGCAT CAGGAGCGGC AACTGCAGCA 
GCAGTCGCAA CAAGCCCTGC AGCAGCGACA ACATTGAAGG CGCCAACAGC AACACAGGCG 
GCAGCAACAA TGGACGACAT CGATGATGAT GATGACGACA GCAACAACGG CGGCCACATG 
CAAAATGCAG GCGTTGGCAA CTTTAAATTC GAGCCAGGCC AACAACTTGG CACGCCGGCG 
AATGAAGCTG TCCTAACGAG TTCCGAGGAG AACAAAGATG GAACTTTGAC TGCTGTTGAC 
ACAAATATGC GCGATGTCAG CGCAAATCAC AGCAGCTCCT TTCTTTCGTC CTCGCCCGCC 
TCCTCGACTC TGGTGTCCTG TCACTCCCCC TCGCCGCCGC TGCCGCTGCT TGCGGACCAG 
GACGATGGAA CGAGGAGGAT GCGGGACAAG AAGCTAACGC TCGAATTGAA GTTGCAATCG 
TGTGATGAAT ATGTTGAGCA GCCAAAGGAT AAAGGAGCCG AACGGCTGCA ACCAGCTCAG 
ATTGCCAACG ACAATGCTCC GTCGCCTCTG CATTTGAAAC TGGAGGAGCA GGATGAGCAG 
GAGCAGGCGG ACGTAAAGGA TAAGGTCCTG GAACTGACCG GAGCAGCAGT TGCTCTCTAT 
GGCCACTTGA ACAATGCCAG CAAGGATGTG CGAGATTTAG AGCAGTGTCT GAAATTGCAG 
GACCCAAATG ATATCAGTCG CCTGTCGCGT TCGCCATCGC CACTTCAATC GAATGCTTCC 
GATTGCGATG ATAACAACTC GAGTGTGGGC ACCTCGAGCG ATCGCTGCCG ATCTCCTTTG 
AGTCCTGCGC TCTCCTTGAG CCACCAGCAG GCCAAACGCC AGCTGATGTC GCTGCAGCCC 
CACCCGGCAC ACCATCATCA CAATCCGCAC CATTTGAACC ACCTGAACCA CCATCAGTAC 
AAACAGGAGG AGGATTACGA TGACGCCAAT GGTGGGGCAT TGAATTTAAC CAGCGACAAT 
AGTCGCCACA GCACTCAGTC TCCATCGAAT TCGGTGAAAT CGGCCACAGC ATCGCCGGTT 
CCGGTAATTT CAGTGCCCTC GCCAGTGCCG CCCATGATCT CTCCGGTTTT GGCTCCCTCG 
GGTTGTGGCT CCACCACACC CAATTCCATG GCAGCAGCGG CTGCAGCCGC CGCCGCTGTG 
GCATCCACCA TGGGTAGTGG CATCTCGCCG TTGCTGGCCC TGCCGGGAAT GTCCTCGCCA 
CAGGCTCAAC TCGCAGCAGC TGGCTTGGGC ATGAACAATC CACTGCTGAC TGGATCACTG 
TCGCCACAGG ATTTTGCCCA GTTCCATCAG CTATTGCAGC AACGTCAAGT TGCGTTGACA 
CAGCAGTTCA ACAGCTACAT GGAGCTGCTG CGAAGTGGTT CCCTGGGCCT GGCACAGGAC 
GATCCGGCAC TGACCGCCCA GGTGGCGGCA GCCCAGTTCC TAATGCAGAG CCAACTGCAG 
GCCCTCAGTC AGGCCAGTCA GCAATTGCAG GCACTGCAGA AGCAACAGCA GCGCCAGGTG 
GATGAGCCAC TGCAGCTGAA CCACAAGATG ACGCAACAGC CACGCAGTTC CACACCGCAC 
TCGATCCGCA GCCCCATCGC AATTCGCAGT CCGGCCAGCT CACCCCAGCA GTTGCACCAT 
CACCACCACC ATCCACTGCA AATCACGCCG CCCAGCTCGG CGGCCAGTTT GAAATTGAGT 
GGTATGCTGA CGCCCAGTAC GCCCACCAGT GGCACCCAGA TGAGCCAGGG CACCACCACG 
CCGCAGCCCA AGACGGTGGC CAGTGCTGCA GCTGCTCGGG CAGCGGGCGA GCCATCGCCG 
GAGGAAACCA CCGATCTAGA AGAGTTGGAG CAGTTTGCCA AGACCTTCAA GCAGCGGCGA 
ATCAAGCTGG GCTTCACCCA GGGCGATGTG GGTCTGGCCA TGGGCAAACT GTATGGCAAT 
GACTTCTCGC AGACCACCAT TTCACGTTTC GAGGCCCTCA ATCTGAGCTT CAAGAACATG 
TGCAAGCTGA AGCCGCTGCT CCAGAAGTGG TTGGATGACG CCGATCGCAC CATCCAGGCC 
ACCGGTGGTG TCTTCGATCC TGCCGCCTTG CAGGCCACCG TCAGCACACC GGAGATCATT 
GGTCGCCGTC GCAAGAAGCG CACCTCCATC GAGACCACCA TTCGCGGTGC TCTGGAGAAG 
GCCTTTCTGG CCAACCAGAA GCCCACCTCA GAGGAGATCA CTCAGCTGGC CGATCGTCTG 
AGCATGGAGA AGGAGGTGGT GCGCGTGTGG TTCTGCAACC GTCGGCAGAA GGAGAAGCGC 
ATCAATCCCT CCCTGGACAG TCCCACGGGC GCCGATGACG ACGAGTCCTC CTATATGATG 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nub cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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