mtDNA-helicase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtDNA-helicase
Entrez Gene ID	34307
Full Name	mitochondrial DNA helicase
Synonyms	CG5924,Dmel\CG5924,mtDNA helicase
General protein information	Preferred Names mitochondrial DNA helicase Names CG5924 gene product from transcript CG5924-RA CG5924-PA d-mtDNA helicase mtDNA helicase mtDNA-helicase-PA twinkle
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30E1-30E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135474.3	NP_609318.1	mitochondrial DNA helicase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peo1	NP_722491.2
Rattus norvegicus (Norway rat)	Peo1	NP_001101069.1
Drosophila melanogaster (fruit fly)	CG5924	NP_609318.1
Homo sapiens (human)	C10orf2	NP_068602.2
Pan troglodytes (chimpanzee)	C10H10orf2	XP_003951780.1
Canis lupus familiaris (dog)	C28H10orf2	XP_543974.1
Danio rerio (zebrafish)	peo1	NP_001264527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010720	XP_311434.3
Caenorhabditis elegans (roundworm)	F46G11.1	NP_508973.3
Gallus gallus (chicken)	PEO1	NP_001026515.1
Xenopus tropicalis (tropical clawed frog)	LOC100491229	XP_002938766.1
	C9H10orf2	XP_001109287.1
Bos taurus (cattle)	C26H10orf2	NP_001091933.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the cloning and molecular analysis of Drosophila mitochondrial DNA helicase (d-mtDNA helicase) homologous to human TWINKLE, which encodes one of the genes responsible for autosomal dominant progressive external ophthalmoplegia
Title: Differential phenotypes of active site and human autosomal dominant progressive external ophthalmoplegia mutations in Drosophila mitochondrial DNA helicase expressed in Schneider cells.

The following mtDNA-helicase gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtDNA-helicase cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14239	NM_135474.3 Latest version!	Drosophila melanogaster mitochondrial DNA helicase (mtDNA-helicase), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtDNA-helicase ( NM_135474.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609318.1 Drosophila melanogaster mitochondrial DNA helicase (mtDNA-helicase), mRNA.

Price & Availability↑

CloneID	OFa14239
Clone ID Related Accession (Same CDS sequence)	NM_135474.3
Accession Version	NM_135474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial DNA helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135474.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGACGCG CCGGTTTAAT CAAACCGCTG TTGCGTATAA ATAACGAAAA ACAACGTGTT 
TGGAGAAAAA ACTACGCTAC CCAAGTGGTT TCCGGCTTGG AGGAGTGCTC ACTAGACCCC 
AAGGAGTATG TGGACTTCAA GCGGCAGCTG CGGCAGCTCA ACTTGCCCCA CAAAGACGGA 
CACACGTGCT TGCAGCTGGA GTGTCGCCTC TGCGATCGCA ATAGGCAGCC GGTGACAAAC 
GCCCAAAAGG GCACCGATCA TGGGCTGCTG GCTTACGTAA ACAAGCGGAC GGGAGCCTTT 
ATCTGCCCCA ATTGCGACGT AAAAACCTCA CTGACCAGCG CTTTATTATC CTATCAGTTG 
CCCAAGCCGG TTGGCTATAA GCAACCGCTG CAGAGGCAGC CGGTCTATGA ATCCCGATTT 
CCCCACCTGG CCGTGGTTAC TCCCGAGGCC TGTGCAGCTT TGGGAATCAA GGGCTTGAAG 
GAGGACCAAC TAAATGCCAT AGGAGCACAG TGGGAGCCGC AGCAACAGCT GCTCCACTTT 
AAGTTACGCA ATGCCGCGCA AGTGGAGGTT GGCGAAAAGG TGTTATATCT GGGCGATCGA 
CGGGAGGAGA TCTTCCAGAG CAGCTCCAGC TCCGGGCTGC TGATTCACGG TGCGATGAAC 
AAAACCAAAG CGGTTCTAGT CAGCAATCTA ATTGACTTTA TTGTCCTTGC CACACAGAAC 
ATTGAAACGC ATTGCGTTGT ATGCCTGCCC TACGAACTGA AGACTCTACC ACAAGAGTGT 
CTGCCCGCCT TGGAACGCTT CAAAGAGCTC ATCTTTTGGT TGCACTACGA TGCCAGTCAC 
AGCTGGGATG CAGCTAGAGC TTTTGCGTTA AAGTTGGACG AAAGGCGATG CCTGTTAATC 
CGACCTACTG AGACGGAACC TGCGCCACAT TTGGCTCTGC GCCGACGACT CAATCTGCGC 
CACATCCTGG CCAAAGCGAC ACCGGTGCAG CACAAGGCCA TCACAACATT CGGAGCAATG 
CGAAATGACA TTCTCAGCGA GTTGCAAAAC ATCGAGAAAG TCAATGGAGT AAAGTGGAAG 
CGCTTTCCAG TACTGAATAA GCTGCTCAAA GGCCATCGGA GGGGAGAGTT GACAATCCTC 
ACAGGACCCA CGGGTAGTGG AAAGACAACC TTTATGAGCG AGTACTCTCT GGATCTGGCC 
ATGCAGGGCG TAAACACGCT GTGGGGCTCT TTTGAGATCC GAAACACCCG GCTGGCGGCC 
ACCTTGCTTC GCCAGTATGT GGGCTACCCG TTGGACGACC GGTTGCATGA GTTTAACCAC 
TGGGCAGCGG AGTTTGAGCG TTTGCCGCTA TACTTTATGA CCTTCCACGG ACAGCAGCCC 
CTTAAGCCGG TTCTGGAAGC CATCGAGCAT GCGTCGTATG TGCACGATGT GATGCACGTG 
ATCATTGACA ATCTGCAATT TATGATGGGC GTGTCCACAT TTCGAGGCGA CAAGTTCTTT 
GAACAGGACT CTATTATAGC TGCCTTTCGC TCCTTTGCCA CCAAGCACAA TGTACACGTG 
ACACTCGTAA TGCATCCGCG CAAGGAGCGG CAGGAGGACG AGCTGACCAC CAGCTCGGTT 
TTCGGCACAG CAAAGGCCAC CCAAGAAGCG GACAACGTGT TGATCATTCA AGACAAGAGG 
CTGACTTCTG TGCGGGGCAA GAAGTATCTC CAAATTGCCA AGAATCGTTA TTCCGGCGAC 
CTGGGCATCA TGCCACTGGA ATTCGACAAA GACGGCTTGA GCTACTCAAC CCAGATCCAA 
AATGCCAAGC GAAAACGGGA AAAGACGCCA TCCGAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609318.1
CDS	28..1869
Translation	MRRAGLIKPLLRINNEKQRVWRKNYATQVVSGLEECSLDPKEYVDFKRQLRQLNLPHKDGHTCLQLECRLCDRNRQPVTNAQKGTDHGLLAYVNKRTGAFICPNCDVKTSLTSALLSYQLPKPVGYKQPLQRQPVYESRFPHLAVVTPEACAALGIKGLKEDQLNAIGAQWEPQQQLLHFKLRNAAQVEVGEKVLYLGDRREEIFQSSSSSGLLIHGAMNKTKAVLVSNLIDFIVLATQNIETHCVVCLPYELKTLPQECLPALERFKELIFWLHYDASHSWDAARAFALKLDERRCLLIRPTETEPAPHLALRRRLNLRHILAKATPVQHKAITTFGAMRNDILSELQNIEKVNGVKWKRFPVLNKLLKGHRRGELTILTGPTGSGKTTFMSEYSLDLAMQGVNTLWGSFEIRNTRLAATLLRQYVGYPLDDRLHEFNHWAAEFERLPLYFMTFHGQQPLKPVLEAIEHASYVHDVMHVIIDNLQFMMGVSTFRGDKFFEQDSIIAAFRSFATKHNVHVTLVMHPRKERQEDELTTSSVFGTAKATQEADNVLIIQDKRLTSVRGKKYLQIAKNRYSGDLGIMPLEFDKDGLSYSTQIQNAKRKREKTPSEN

Target ORF information:

RefSeq Version	NM_135474.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial DNA helicase (mtDNA-helicase), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135474.3

ORF Insert Sequence:

ATGAGACGCG CCGGTTTAAT CAAACCGCTG TTGCGTATAA ATAACGAAAA ACAACGTGTT 
TGGAGAAAAA ACTACGCTAC CCAAGTGGTT TCCGGCTTGG AGGAGTGCTC ACTAGACCCC 
AAGGAGTATG TGGACTTCAA GCGGCAGCTG CGGCAGCTCA ACTTGCCCCA CAAAGACGGA 
CACACGTGCT TGCAGCTGGA GTGTCGCCTC TGCGATCGCA ATAGGCAGCC GGTGACAAAC 
GCCCAAAAGG GCACCGATCA TGGGCTGCTG GCTTACGTAA ACAAGCGGAC GGGAGCCTTT 
ATCTGCCCCA ATTGCGACGT AAAAACCTCA CTGACCAGCG CTTTATTATC CTATCAGTTG 
CCCAAGCCGG TTGGCTATAA GCAACCGCTG CAGAGGCAGC CGGTCTATGA ATCCCGATTT 
CCCCACCTGG CCGTGGTTAC TCCCGAGGCC TGTGCAGCTT TGGGAATCAA GGGCTTGAAG 
GAGGACCAAC TAAATGCCAT AGGAGCACAG TGGGAGCCGC AGCAACAGCT GCTCCACTTT 
AAGTTACGCA ATGCCGCGCA AGTGGAGGTT GGCGAAAAGG TGTTATATCT GGGCGATCGA 
CGGGAGGAGA TCTTCCAGAG CAGCTCCAGC TCCGGGCTGC TGATTCACGG TGCGATGAAC 
AAAACCAAAG CGGTTCTAGT CAGCAATCTA ATTGACTTTA TTGTCCTTGC CACACAGAAC 
ATTGAAACGC ATTGCGTTGT ATGCCTGCCC TACGAACTGA AGACTCTACC ACAAGAGTGT 
CTGCCCGCCT TGGAACGCTT CAAAGAGCTC ATCTTTTGGT TGCACTACGA TGCCAGTCAC 
AGCTGGGATG CAGCTAGAGC TTTTGCGTTA AAGTTGGACG AAAGGCGATG CCTGTTAATC 
CGACCTACTG AGACGGAACC TGCGCCACAT TTGGCTCTGC GCCGACGACT CAATCTGCGC 
CACATCCTGG CCAAAGCGAC ACCGGTGCAG CACAAGGCCA TCACAACATT CGGAGCAATG 
CGAAATGACA TTCTCAGCGA GTTGCAAAAC ATCGAGAAAG TCAATGGAGT AAAGTGGAAG 
CGCTTTCCAG TACTGAATAA GCTGCTCAAA GGCCATCGGA GGGGAGAGTT GACAATCCTC 
ACAGGACCCA CGGGTAGTGG AAAGACAACC TTTATGAGCG AGTACTCTCT GGATCTGGCC 
ATGCAGGGCG TAAACACGCT GTGGGGCTCT TTTGAGATCC GAAACACCCG GCTGGCGGCC 
ACCTTGCTTC GCCAGTATGT GGGCTACCCG TTGGACGACC GGTTGCATGA GTTTAACCAC 
TGGGCAGCGG AGTTTGAGCG TTTGCCGCTA TACTTTATGA CCTTCCACGG ACAGCAGCCC 
CTTAAGCCGG TTCTGGAAGC CATCGAGCAT GCGTCGTATG TGCACGATGT GATGCACGTG 
ATCATTGACA ATCTGCAATT TATGATGGGC GTGTCCACAT TTCGAGGCGA CAAGTTCTTT 
GAACAGGACT CTATTATAGC TGCCTTTCGC TCCTTTGCCA CCAAGCACAA TGTACACGTG 
ACACTCGTAA TGCATCCGCG CAAGGAGCGG CAGGAGGACG AGCTGACCAC CAGCTCGGTT 
TTCGGCACAG CAAAGGCCAC CCAAGAAGCG GACAACGTGT TGATCATTCA AGACAAGAGG 
CTGACTTCTG TGCGGGGCAA GAAGTATCTC CAAATTGCCA AGAATCGTTA TTCCGGCGAC 
CTGGGCATCA TGCCACTGGA ATTCGACAAA GACGGCTTGA GCTACTCAAC CCAGATCCAA 
AATGCCAAGC GAAAACGGGA AAAGACGCCA TCCGAGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mtDNA-helicase cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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