TWNK cDNA ORF clone, Canis lupus familiaris(dog)

The following TWNK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWNK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe27900 XM_005637625.3
Latest version!
Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe283359 XM_022412115.1
Latest version!
Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OCe27899 XM_543974.5
Latest version!
Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe283357 XM_022412113.1
Latest version!
Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe283358 XM_022412114.1
Latest version!
Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe27900 XM_005637625.2 Canis lupus familiaris chromosome 28 open reading frame, human C10orf2 (C28H10orf2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe27899 XM_543974.4 Canis lupus familiaris chromosome 28 open reading frame, human C10orf2 (C28H10orf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe27900
    Clone ID Related Accession (Same CDS sequence) XM_005637625.2 , XM_005637625.3
    Accession Version XM_005637625.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product twinkle protein, mitochondrial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTCCTTG GTGCGGCCTG GGGACCAGCA GCCCCGTCCC CTGGAGGCCC TGAACCGAGG 
    TTTGAATCTT TCTCGTATTC TGCGGACTGC CCTGCCTGCC TGGCACAAGT CTATCGTGTC
    TTTCCGGCAG CTTCGGGAGG AGGTGCTAGG GGAACTGTCA AATGTGGAGC AGGCAGCTGG
    CATCCGCTGG AACCGCTTCC CAGACCTCAA TCGTCTCTTG AAGGGACATC GGAAGGGCGA
    GCTGACAGTC TTCACGGGTC TGTGTTCAAC CCCCCTGCAG GGCCAACAGG CAGTGGAAAG
    ACGACATTCA TCAGTGAGTA TGCCCTGGAT TTGTGTACCC AGGGGGTAAA CACACTGTGG
    GGTAGCTTTG AGATCAGCAA CGTGAGACTA GCCCGAGTCA TGCTGACACA GTTCGCTGTG
    GGGCGGCTAG AAGAGCAGTT GGACAAATAC GACGAGTGGG CCGACCGCTT TGAAGACCTG
    CCTCTCTATT TCATGACTTT CCATGGGCAG CAGAGCATCA GGACGGTAAT AGACACAATG
    CAACATGCAG TATACGTTTA TGACATCTGC CATGTGGTCA TCGACAATCT GCAGTTCATG
    ATGGGGCATG AGCAGTTGTC CACAGACAGG ATTGCAGCTC AAGACTACAT CGTTGGGGCC
    TTTCGAAAGT TTGCAACAGA CAATAGCTGC CATGTGACAC TGGTCATTCA TCCCCGGAAA
    GAGGATGATG ATAAGGAACT GCAGACAGCA TCAATTTTTG GCTCAGCCAA AGCAAGCCAG
    GAAGCAGACA ATGTTCTGAT CCTACAGGAC AGGAAGCTGG TAACTGGTCC AGGGAAACGG
    TATCTGCAGG TGTCCAAGAA CCGCTTTGAT GGAGACGTAG GTGTCTTTCC ACTTGAATTC
    AACAAGAGCT CTCTTACCTT CTCCATACCA CCCAAGAGCA AGGCGCGGCT CAAGAAGGTC
    AAGGATGACA ACGGGCTTGT GACCAAGAAG CCGTCCTCTG GCAAAAAAGG GGCTGTGCCC
    CAGAATTCTG AGACTTGCTT GGGTCAGTCC CACCACCCCT ATCAGCCAGA CGTCTCCAAG
    CCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637682.1
    CDS100..1191
    Translation

    Target ORF information:

    RefSeq Version XM_005637625.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris chromosome 28 open reading frame, human C10orf2 (C28H10orf2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTCCTTG GTGCGGCCTG GGGACCAGCA GCCCCGTCCC CTGGAGGCCC TGAACCGAGG 
    TTTGAATCTT TCTCGTATTC TGCGGACTGC CCTGCCTGCC TGGCACAAGT CTATCGTGTC
    TTTCCGGCAG CTTCGGGAGG AGGTGCTAGG GGAACTGTCA AATGTGGAGC AGGCAGCTGG
    CATCCGCTGG AACCGCTTCC CAGACCTCAA TCGTCTCTTG AAGGGACATC GGAAGGGCGA
    GCTGACAGTC TTCACGGGTC TGTGTTCAAC CCCCCTGCAG GGCCAACAGG CAGTGGAAAG
    ACGACATTCA TCAGTGAGTA TGCCCTGGAT TTGTGTACCC AGGGGGTAAA CACACTGTGG
    GGTAGCTTTG AGATCAGCAA CGTGAGACTA GCCCGAGTCA TGCTGACACA GTTCGCTGTG
    GGGCGGCTAG AAGAGCAGTT GGACAAATAC GACGAGTGGG CCGACCGCTT TGAAGACCTG
    CCTCTCTATT TCATGACTTT CCATGGGCAG CAGAGCATCA GGACGGTAAT AGACACAATG
    CAACATGCAG TATACGTTTA TGACATCTGC CATGTGGTCA TCGACAATCT GCAGTTCATG
    ATGGGGCATG AGCAGTTGTC CACAGACAGG ATTGCAGCTC AAGACTACAT CGTTGGGGCC
    TTTCGAAAGT TTGCAACAGA CAATAGCTGC CATGTGACAC TGGTCATTCA TCCCCGGAAA
    GAGGATGATG ATAAGGAACT GCAGACAGCA TCAATTTTTG GCTCAGCCAA AGCAAGCCAG
    GAAGCAGACA ATGTTCTGAT CCTACAGGAC AGGAAGCTGG TAACTGGTCC AGGGAAACGG
    TATCTGCAGG TGTCCAAGAA CCGCTTTGAT GGAGACGTAG GTGTCTTTCC ACTTGAATTC
    AACAAGAGCT CTCTTACCTT CTCCATACCA CCCAAGAGCA AGGCGCGGCT CAAGAAGGTC
    AAGGATGACA ACGGGCTTGT GACCAAGAAG CCGTCCTCTG GCAAAAAAGG GGCTGTGCCC
    CAGAATTCTG AGACTTGCTT GGGTCAGTCC CACCACCCCT ATCAGCCAGA CGTCTCCAAG
    CCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27900
    Clone ID Related Accession (Same CDS sequence) XM_005637625.2 , XM_005637625.3
    Accession Version XM_005637625.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product twinkle protein, mitochondrial isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTCCTTG GTGCGGCCTG GGGACCAGCA GCCCCGTCCC CTGGAGGCCC TGAACCGAGG 
    TTTGAATCTT TCTCGTATTC TGCGGACTGC CCTGCCTGCC TGGCACAAGT CTATCGTGTC
    TTTCCGGCAG CTTCGGGAGG AGGTGCTAGG GGAACTGTCA AATGTGGAGC AGGCAGCTGG
    CATCCGCTGG AACCGCTTCC CAGACCTCAA TCGTCTCTTG AAGGGACATC GGAAGGGCGA
    GCTGACAGTC TTCACGGGTC TGTGTTCAAC CCCCCTGCAG GGCCAACAGG CAGTGGAAAG
    ACGACATTCA TCAGTGAGTA TGCCCTGGAT TTGTGTACCC AGGGGGTAAA CACACTGTGG
    GGTAGCTTTG AGATCAGCAA CGTGAGACTA GCCCGAGTCA TGCTGACACA GTTCGCTGTG
    GGGCGGCTAG AAGAGCAGTT GGACAAATAC GACGAGTGGG CCGACCGCTT TGAAGACCTG
    CCTCTCTATT TCATGACTTT CCATGGGCAG CAGAGCATCA GGACGGTAAT AGACACAATG
    CAACATGCAG TATACGTTTA TGACATCTGC CATGTGGTCA TCGACAATCT GCAGTTCATG
    ATGGGGCATG AGCAGTTGTC CACAGACAGG ATTGCAGCTC AAGACTACAT CGTTGGGGCC
    TTTCGAAAGT TTGCAACAGA CAATAGCTGC CATGTGACAC TGGTCATTCA TCCCCGGAAA
    GAGGATGATG ATAAGGAACT GCAGACAGCA TCAATTTTTG GCTCAGCCAA AGCAAGCCAG
    GAAGCAGACA ATGTTCTGAT CCTACAGGAC AGGAAGCTGG TAACTGGTCC AGGGAAACGG
    TATCTGCAGG TGTCCAAGAA CCGCTTTGAT GGAGACGTAG GTGTCTTTCC ACTTGAATTC
    AACAAGAGCT CTCTTACCTT CTCCATACCA CCCAAGAGCA AGGCGCGGCT CAAGAAGGTC
    AAGGATGACA ACGGGCTTGT GACCAAGAAG CCGTCCTCTG GCAAAAAAGG GGCTGTGCCC
    CAGAATTCTG AGACTTGCTT GGGTCAGTCC CACCACCCCT ATCAGCCAGA CGTCTCCAAG
    CCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637682.1
    CDS143..1234
    Translation

    Target ORF information:

    RefSeq Version XM_005637625.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637625.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTCCTTG GTGCGGCCTG GGGACCAGCA GCCCCGTCCC CTGGAGGCCC TGAACCGAGG 
    TTTGAATCTT TCTCGTATTC TGCGGACTGC CCTGCCTGCC TGGCACAAGT CTATCGTGTC
    TTTCCGGCAG CTTCGGGAGG AGGTGCTAGG GGAACTGTCA AATGTGGAGC AGGCAGCTGG
    CATCCGCTGG AACCGCTTCC CAGACCTCAA TCGTCTCTTG AAGGGACATC GGAAGGGCGA
    GCTGACAGTC TTCACGGGTC TGTGTTCAAC CCCCCTGCAG GGCCAACAGG CAGTGGAAAG
    ACGACATTCA TCAGTGAGTA TGCCCTGGAT TTGTGTACCC AGGGGGTAAA CACACTGTGG
    GGTAGCTTTG AGATCAGCAA CGTGAGACTA GCCCGAGTCA TGCTGACACA GTTCGCTGTG
    GGGCGGCTAG AAGAGCAGTT GGACAAATAC GACGAGTGGG CCGACCGCTT TGAAGACCTG
    CCTCTCTATT TCATGACTTT CCATGGGCAG CAGAGCATCA GGACGGTAAT AGACACAATG
    CAACATGCAG TATACGTTTA TGACATCTGC CATGTGGTCA TCGACAATCT GCAGTTCATG
    ATGGGGCATG AGCAGTTGTC CACAGACAGG ATTGCAGCTC AAGACTACAT CGTTGGGGCC
    TTTCGAAAGT TTGCAACAGA CAATAGCTGC CATGTGACAC TGGTCATTCA TCCCCGGAAA
    GAGGATGATG ATAAGGAACT GCAGACAGCA TCAATTTTTG GCTCAGCCAA AGCAAGCCAG
    GAAGCAGACA ATGTTCTGAT CCTACAGGAC AGGAAGCTGG TAACTGGTCC AGGGAAACGG
    TATCTGCAGG TGTCCAAGAA CCGCTTTGAT GGAGACGTAG GTGTCTTTCC ACTTGAATTC
    AACAAGAGCT CTCTTACCTT CTCCATACCA CCCAAGAGCA AGGCGCGGCT CAAGAAGGTC
    AAGGATGACA ACGGGCTTGT GACCAAGAAG CCGTCCTCTG GCAAAAAAGG GGCTGTGCCC
    CAGAATTCTG AGACTTGCTT GGGTCAGTCC CACCACCCCT ATCAGCCAGA CGTCTCCAAG
    CCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283359
    Clone ID Related Accession (Same CDS sequence) XM_022412115.1
    Accession Version XM_022412115.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product twinkle protein, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGTCT GTCAGCTGGG CTGCAGGGCC CCCACCCCCA TTTCCTGCTA TGTGTGTTCC 
    GCTTGGTCTA GACTGGCCTT CCTGATCACT AGGCTTCTGA GCCTATGGAA ACAGTGCTGC
    AGGGTTGGTT CTGCTCCCCA TAAAACCATT TGCAGAGAAA ACTGGGATCC TCAGCTTCCA
    TTTGGCTGCT CCAGGCAGCT TCGGGAGGAG GTGCTAGGGG AACTGTCAAA TGTGGAGCAG
    GCAGCTGGCA TCCGCTGGAA CCGCTTCCCA GACCTCAATC GTCTCTTGAA GGGACATCGG
    AAGGGCGAGC TGACAGTCTT CACGGGTGAG CCCTTGGGAA ATCACTGCTT GGGGTCTGTG
    TTCAACCCCC CTGCAGGGCC AACAGGCAGT GGAAAGACGA CATTCATCAG TGAGTATGCC
    CTGGATTTGT GTACCCAGGG GGTAAACACA CTGTGGGGTA GCTTTGAGAT CAGCAACGTG
    AGACTAGCCC GAGTCATGCT GACACAGTTC GCTGTGGGGC GGCTAGAAGA GCAGTTGGAC
    AAATACGACG AGTGGGCCGA CCGCTTTGAA GACCTGCCTC TCTATTTCAT GACTTTCCAT
    GGGCAGCAGA GCATCAGGAC GGTAATAGAC ACAATGCAAC ATGCAGTATA CGTTTATGAC
    ATCTGCCATG TGGTCATCGA CAATCTGCAG TTCATGATGG GGCATGAGCA GTTGTCCACA
    GACAGGATTG CAGCTCAAGA CTACATCGTT GGGGCCTTTC GAAAGTTTGC AACAGACAAT
    AGCTGCCATG TGACACTGGT CATTCATCCC CGGAAAGAGG ATGATGATAA GGAACTGCAG
    ACAGCATCAA TTTTTGGCTC AGCCAAAGCA AGCCAGGAAG CAGACAATGT TCTGATCCTA
    CAGGACAGGA AGCTGGTAAC TGGTCCAGGG AAACGGTATC TGCAGGTGTC CAAGAACCGC
    TTTGATGGAG ACGTAGGTGT CTTTCCACTT GAATTCAACA AGAGCTCTCT TACCTTCTCC
    ATACCACCCA AGAGCAAGGC GCGGCTCAAG AAGGTCAAGG ATGACAACGG GCTTGTGACC
    AAGAAGCCGT CCTCTGGCAA AAAAGGGGCT GTGCCCCAGA ATTCTGAGAC TTGCTTGGGT
    CAGTCCCACC ACCCCTATCA GCCAGACGTC TCCAAGCCCT CAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267823.1
    CDS86..1273
    Translation

    Target ORF information:

    RefSeq Version XM_022412115.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGTCT GTCAGCTGGG CTGCAGGGCC CCCACCCCCA TTTCCTGCTA TGTGTGTTCC 
    GCTTGGTCTA GACTGGCCTT CCTGATCACT AGGCTTCTGA GCCTATGGAA ACAGTGCTGC
    AGGGTTGGTT CTGCTCCCCA TAAAACCATT TGCAGAGAAA ACTGGGATCC TCAGCTTCCA
    TTTGGCTGCT CCAGGCAGCT TCGGGAGGAG GTGCTAGGGG AACTGTCAAA TGTGGAGCAG
    GCAGCTGGCA TCCGCTGGAA CCGCTTCCCA GACCTCAATC GTCTCTTGAA GGGACATCGG
    AAGGGCGAGC TGACAGTCTT CACGGGTGAG CCCTTGGGAA ATCACTGCTT GGGGTCTGTG
    TTCAACCCCC CTGCAGGGCC AACAGGCAGT GGAAAGACGA CATTCATCAG TGAGTATGCC
    CTGGATTTGT GTACCCAGGG GGTAAACACA CTGTGGGGTA GCTTTGAGAT CAGCAACGTG
    AGACTAGCCC GAGTCATGCT GACACAGTTC GCTGTGGGGC GGCTAGAAGA GCAGTTGGAC
    AAATACGACG AGTGGGCCGA CCGCTTTGAA GACCTGCCTC TCTATTTCAT GACTTTCCAT
    GGGCAGCAGA GCATCAGGAC GGTAATAGAC ACAATGCAAC ATGCAGTATA CGTTTATGAC
    ATCTGCCATG TGGTCATCGA CAATCTGCAG TTCATGATGG GGCATGAGCA GTTGTCCACA
    GACAGGATTG CAGCTCAAGA CTACATCGTT GGGGCCTTTC GAAAGTTTGC AACAGACAAT
    AGCTGCCATG TGACACTGGT CATTCATCCC CGGAAAGAGG ATGATGATAA GGAACTGCAG
    ACAGCATCAA TTTTTGGCTC AGCCAAAGCA AGCCAGGAAG CAGACAATGT TCTGATCCTA
    CAGGACAGGA AGCTGGTAAC TGGTCCAGGG AAACGGTATC TGCAGGTGTC CAAGAACCGC
    TTTGATGGAG ACGTAGGTGT CTTTCCACTT GAATTCAACA AGAGCTCTCT TACCTTCTCC
    ATACCACCCA AGAGCAAGGC GCGGCTCAAG AAGGTCAAGG ATGACAACGG GCTTGTGACC
    AAGAAGCCGT CCTCTGGCAA AAAAGGGGCT GTGCCCCAGA ATTCTGAGAC TTGCTTGGGT
    CAGTCCCACC ACCCCTATCA GCCAGACGTC TCCAAGCCCT CAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27899
    Clone ID Related Accession (Same CDS sequence) XM_543974.4 , XM_543974.5
    Accession Version XM_543974.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product twinkle protein, mitochondrial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTA CCCAGGGGGT AAACACACTG
    TGGGGTAGCT TTGAGATCAG CAACGTGAGA CTAGCCCGAG TCATGCTGAC ACAGTTCGCT
    GTGGGGCGGC TAGAAGAGCA GTTGGACAAA TACGACGAGT GGGCCGACCG CTTTGAAGAC
    CTGCCTCTCT ATTTCATGAC TTTCCATGGG CAGCAGAGCA TCAGGACGGT AATAGACACA
    ATGCAACATG CAGTATACGT TTATGACATC TGCCATGTGG TCATCGACAA TCTGCAGTTC
    ATGATGGGGC ATGAGCAGTT GTCCACAGAC AGGATTGCAG CTCAAGACTA CATCGTTGGG
    GCCTTTCGAA AGTTTGCAAC AGACAATAGC TGCCATGTGA CACTGGTCAT TCATCCCCGG
    AAAGAGGATG ATGATAAGGA ACTGCAGACA GCATCAATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTACAG GACAGGAAGC TGGTAACTGG TCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGACG TAGGTGTCTT TCCACTTGAA
    TTCAACAAGA GCTCTCTTAC CTTCTCCATA CCACCCAAGA GCAAGGCGCG GCTCAAGAAG
    GTCAAGGATG ACAACGGGCT TGTGACCAAG AAGCCGTCCT CTGGCAAAAA AGGGGCTGTG
    CCCCAGAATT CTGAGACTTG CTTGGGTCAG TCCCACCACC CCTATCAGCC AGACGTCTCC
    AAGCCCTCAA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543974.1
    CDS617..2671
    Translation

    Target ORF information:

    RefSeq Version XM_543974.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris chromosome 28 open reading frame, human C10orf2 (C28H10orf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543974.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTA CCCAGGGGGT AAACACACTG
    TGGGGTAGCT TTGAGATCAG CAACGTGAGA CTAGCCCGAG TCATGCTGAC ACAGTTCGCT
    GTGGGGCGGC TAGAAGAGCA GTTGGACAAA TACGACGAGT GGGCCGACCG CTTTGAAGAC
    CTGCCTCTCT ATTTCATGAC TTTCCATGGG CAGCAGAGCA TCAGGACGGT AATAGACACA
    ATGCAACATG CAGTATACGT TTATGACATC TGCCATGTGG TCATCGACAA TCTGCAGTTC
    ATGATGGGGC ATGAGCAGTT GTCCACAGAC AGGATTGCAG CTCAAGACTA CATCGTTGGG
    GCCTTTCGAA AGTTTGCAAC AGACAATAGC TGCCATGTGA CACTGGTCAT TCATCCCCGG
    AAAGAGGATG ATGATAAGGA ACTGCAGACA GCATCAATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTACAG GACAGGAAGC TGGTAACTGG TCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGACG TAGGTGTCTT TCCACTTGAA
    TTCAACAAGA GCTCTCTTAC CTTCTCCATA CCACCCAAGA GCAAGGCGCG GCTCAAGAAG
    GTCAAGGATG ACAACGGGCT TGTGACCAAG AAGCCGTCCT CTGGCAAAAA AGGGGCTGTG
    CCCCAGAATT CTGAGACTTG CTTGGGTCAG TCCCACCACC CCTATCAGCC AGACGTCTCC
    AAGCCCTCAA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27899
    Clone ID Related Accession (Same CDS sequence) XM_543974.4 , XM_543974.5
    Accession Version XM_543974.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product twinkle protein, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543974.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTA CCCAGGGGGT AAACACACTG
    TGGGGTAGCT TTGAGATCAG CAACGTGAGA CTAGCCCGAG TCATGCTGAC ACAGTTCGCT
    GTGGGGCGGC TAGAAGAGCA GTTGGACAAA TACGACGAGT GGGCCGACCG CTTTGAAGAC
    CTGCCTCTCT ATTTCATGAC TTTCCATGGG CAGCAGAGCA TCAGGACGGT AATAGACACA
    ATGCAACATG CAGTATACGT TTATGACATC TGCCATGTGG TCATCGACAA TCTGCAGTTC
    ATGATGGGGC ATGAGCAGTT GTCCACAGAC AGGATTGCAG CTCAAGACTA CATCGTTGGG
    GCCTTTCGAA AGTTTGCAAC AGACAATAGC TGCCATGTGA CACTGGTCAT TCATCCCCGG
    AAAGAGGATG ATGATAAGGA ACTGCAGACA GCATCAATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTACAG GACAGGAAGC TGGTAACTGG TCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGACG TAGGTGTCTT TCCACTTGAA
    TTCAACAAGA GCTCTCTTAC CTTCTCCATA CCACCCAAGA GCAAGGCGCG GCTCAAGAAG
    GTCAAGGATG ACAACGGGCT TGTGACCAAG AAGCCGTCCT CTGGCAAAAA AGGGGCTGTG
    CCCCAGAATT CTGAGACTTG CTTGGGTCAG TCCCACCACC CCTATCAGCC AGACGTCTCC
    AAGCCCTCAA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543974.1
    CDS657..2711
    Translation

    Target ORF information:

    RefSeq Version XM_543974.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543974.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTA CCCAGGGGGT AAACACACTG
    TGGGGTAGCT TTGAGATCAG CAACGTGAGA CTAGCCCGAG TCATGCTGAC ACAGTTCGCT
    GTGGGGCGGC TAGAAGAGCA GTTGGACAAA TACGACGAGT GGGCCGACCG CTTTGAAGAC
    CTGCCTCTCT ATTTCATGAC TTTCCATGGG CAGCAGAGCA TCAGGACGGT AATAGACACA
    ATGCAACATG CAGTATACGT TTATGACATC TGCCATGTGG TCATCGACAA TCTGCAGTTC
    ATGATGGGGC ATGAGCAGTT GTCCACAGAC AGGATTGCAG CTCAAGACTA CATCGTTGGG
    GCCTTTCGAA AGTTTGCAAC AGACAATAGC TGCCATGTGA CACTGGTCAT TCATCCCCGG
    AAAGAGGATG ATGATAAGGA ACTGCAGACA GCATCAATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTACAG GACAGGAAGC TGGTAACTGG TCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGACG TAGGTGTCTT TCCACTTGAA
    TTCAACAAGA GCTCTCTTAC CTTCTCCATA CCACCCAAGA GCAAGGCGCG GCTCAAGAAG
    GTCAAGGATG ACAACGGGCT TGTGACCAAG AAGCCGTCCT CTGGCAAAAA AGGGGCTGTG
    CCCCAGAATT CTGAGACTTG CTTGGGTCAG TCCCACCACC CCTATCAGCC AGACGTCTCC
    AAGCCCTCAA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283357
    Clone ID Related Accession (Same CDS sequence) XM_022412113.1
    Accession Version XM_022412113.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product twinkle protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGTGAGCC CTTGGGAAAT
    CACTGCTTGG GGTCTGTGTT CAACCCCCCT GCAGGGCCAA CAGGCAGTGG AAAGACGACA
    TTCATCAGTG AGTATGCCCT GGATTTGTGT ACCCAGGGGG TAAACACACT GTGGGGTAGC
    TTTGAGATCA GCAACGTGAG ACTAGCCCGA GTCATGCTGA CACAGTTCGC TGTGGGGCGG
    CTAGAAGAGC AGTTGGACAA ATACGACGAG TGGGCCGACC GCTTTGAAGA CCTGCCTCTC
    TATTTCATGA CTTTCCATGG GCAGCAGAGC ATCAGGACGG TAATAGACAC AATGCAACAT
    GCAGTATACG TTTATGACAT CTGCCATGTG GTCATCGACA ATCTGCAGTT CATGATGGGG
    CATGAGCAGT TGTCCACAGA CAGGATTGCA GCTCAAGACT ACATCGTTGG GGCCTTTCGA
    AAGTTTGCAA CAGACAATAG CTGCCATGTG ACACTGGTCA TTCATCCCCG GAAAGAGGAT
    GATGATAAGG AACTGCAGAC AGCATCAATT TTTGGCTCAG CCAAAGCAAG CCAGGAAGCA
    GACAATGTTC TGATCCTACA GGACAGGAAG CTGGTAACTG GTCCAGGGAA ACGGTATCTG
    CAGGTGTCCA AGAACCGCTT TGATGGAGAC GTAGGTGTCT TTCCACTTGA ATTCAACAAG
    AGCTCTCTTA CCTTCTCCAT ACCACCCAAG AGCAAGGCGC GGCTCAAGAA GGTCAAGGAT
    GACAACGGGC TTGTGACCAA GAAGCCGTCC TCTGGCAAAA AAGGGGCTGT GCCCCAGAAT
    TCTGAGACTT GCTTGGGTCA GTCCCACCAC CCCTATCAGC CAGACGTCTC CAAGCCCTCA
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267821.1
    CDS657..2762
    Translation

    Target ORF information:

    RefSeq Version XM_022412113.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGTGAGCC CTTGGGAAAT
    CACTGCTTGG GGTCTGTGTT CAACCCCCCT GCAGGGCCAA CAGGCAGTGG AAAGACGACA
    TTCATCAGTG AGTATGCCCT GGATTTGTGT ACCCAGGGGG TAAACACACT GTGGGGTAGC
    TTTGAGATCA GCAACGTGAG ACTAGCCCGA GTCATGCTGA CACAGTTCGC TGTGGGGCGG
    CTAGAAGAGC AGTTGGACAA ATACGACGAG TGGGCCGACC GCTTTGAAGA CCTGCCTCTC
    TATTTCATGA CTTTCCATGG GCAGCAGAGC ATCAGGACGG TAATAGACAC AATGCAACAT
    GCAGTATACG TTTATGACAT CTGCCATGTG GTCATCGACA ATCTGCAGTT CATGATGGGG
    CATGAGCAGT TGTCCACAGA CAGGATTGCA GCTCAAGACT ACATCGTTGG GGCCTTTCGA
    AAGTTTGCAA CAGACAATAG CTGCCATGTG ACACTGGTCA TTCATCCCCG GAAAGAGGAT
    GATGATAAGG AACTGCAGAC AGCATCAATT TTTGGCTCAG CCAAAGCAAG CCAGGAAGCA
    GACAATGTTC TGATCCTACA GGACAGGAAG CTGGTAACTG GTCCAGGGAA ACGGTATCTG
    CAGGTGTCCA AGAACCGCTT TGATGGAGAC GTAGGTGTCT TTCCACTTGA ATTCAACAAG
    AGCTCTCTTA CCTTCTCCAT ACCACCCAAG AGCAAGGCGC GGCTCAAGAA GGTCAAGGAT
    GACAACGGGC TTGTGACCAA GAAGCCGTCC TCTGGCAAAA AAGGGGCTGT GCCCCAGAAT
    TCTGAGACTT GCTTGGGTCA GTCCCACCAC CCCTATCAGC CAGACGTCTC CAAGCCCTCA
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283358
    Clone ID Related Accession (Same CDS sequence) XM_022412114.1
    Accession Version XM_022412114.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product twinkle protein, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGTCTGTG TTCAACCCCC
    CTGCAGGGCC AACAGGCAGT GGAAAGACGA CATTCATCAT TCGCTGTGGG GCGGCTAGAA
    GAGCAGTTGG ACAAATACGA CGAGTGGGCC GACCGCTTTG AAGACCTGCC TCTCTATTTC
    ATGACTTTCC ATGGGCAGCA GAGCATCAGG ACGGTAATAG ACACAATGCA ACATGCAGTA
    TACGTTTATG ACATCTGCCA TGTGGTCATC GACAATCTGC AGTTCATGAT GGGGCATGAG
    CAGTTGTCCA CAGACAGGAT TGCAGCTCAA GACTACATCG TTGGGGCCTT TCGAAAGTTT
    GCAACAGACA ATAGCTGCCA TGTGACACTG GTCATTCATC CCCGGAAAGA GGATGATGAT
    AAGGAACTGC AGACAGCATC AATTTTTGGC TCAGCCAAAG CAAGCCAGGA AGCAGACAAT
    GTTCTGATCC TACAGGACAG GAAGCTGGTA ACTGGTCCAG GGAAACGGTA TCTGCAGGTG
    TCCAAGAACC GCTTTGATGG AGACGTAGGT GTCTTTCCAC TTGAATTCAA CAAGAGCTCT
    CTTACCTTCT CCATACCACC CAAGAGCAAG GCGCGGCTCA AGAAGGTCAA GGATGACAAC
    GGGCTTGTGA CCAAGAAGCC GTCCTCTGGC AAAAAAGGGG CTGTGCCCCA GAATTCTGAG
    ACTTGCTTGG GTCAGTCCCA CCACCCCTAT CAGCCAGACG TCTCCAAGCC CTCAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267822.1
    CDS657..2636
    Translation

    Target ORF information:

    RefSeq Version XM_022412114.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris twinkle mtDNA helicase (TWNK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412114.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGGGTAC TCCTTCGAGG CGGGTACCCC CTCCGTATGC TGCTCCCCCT GCGTGCGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCAGAAGC TTGGCCCCAG GCCCTCCTCG CAGGCGTTAC
    AGGAAGGAGG CTCTCCCAGC CTTGGAGGTA CCAGTGTTGC CCGTAACTGC AACTGAAATC
    CGCCAATATT TGCGGGCACA TGGGATCCCC TTCCAGGATG GACACAGCTG CCTGCGGGCA
    CCGAGCCCCT TTGTGAAGGC CTTGGAGCTC AAGGACCAGA GTGGTGCTAC TGCTTCCTTC
    AGCCTTTTCA TTGACAAGAC CACAGGCCGC TTTCTCTGCA TGACCAGCCT AGCAGAGGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCGAG AGAAGGGATC
    CTGCTCGGTG AGGCCCCAGA AGCCGAGGAC AGTGAGGAGG TCCGGAGGAT CTGGGACCGA
    GCCGTACCTC TCTGGGAGCT GCCTGAGCCG GTGGAGGGCC AGCTGGCTCG TGTGATGTTT
    GGCCTTACCA AAGTGACTGA TGACACGCTC AGGCGTTTCA ATGTGCGGTA TTTGTGGTCT
    GCTCGCAGCC TTGTCTTCCC TTGGCTCTCC CCTGGAGGTT TGGGATTACG AGGCCTGAAG
    CTACTAGGTG CCGAAGGCCA GGGAGACGGA GTGCAGTATA AGGAAACCAC CATTCCCCGG
    CCTGGTGTCT ACTACAATCT GTTTGGATTA CCACTGATCA GTCGGCGAGA TGTTGAGGTG
    GTATTGACGA GTCGTGAGCT GGACAGCTTG GCCTTAAACC AAGCCACAGG GCTGCCCACC
    CTGGCCCTGC CCCGAGGAAT AGCCTGCTTA CCCCCTGCCT TGCTCCCTTA CCTCGAACAG
    TTTCGACGTA TCGTGCTCTG GCTGGGAGAT GACCTTCGGT CCTGGGAAGC TGCCAAGTTG
    TTTGCCCGAA AACTGAATCC CAAGCGATGC TCCTTGGTGC GGCCTGGGGA CCAGCAGCCC
    CGTCCCCTGG AGGCCCTGAA CCGAGGTTTG AATCTTTCTC GTATTCTGCG GACTGCCCTG
    CCTGCCTGGC ACAAGTCTAT CGTGTCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGGGAA
    CTGTCAAATG TGGAGCAGGC AGCTGGCATC CGCTGGAACC GCTTCCCAGA CCTCAATCGT
    CTCTTGAAGG GACATCGGAA GGGCGAGCTG ACAGTCTTCA CGGGTCTGTG TTCAACCCCC
    CTGCAGGGCC AACAGGCAGT GGAAAGACGA CATTCATCAT TCGCTGTGGG GCGGCTAGAA
    GAGCAGTTGG ACAAATACGA CGAGTGGGCC GACCGCTTTG AAGACCTGCC TCTCTATTTC
    ATGACTTTCC ATGGGCAGCA GAGCATCAGG ACGGTAATAG ACACAATGCA ACATGCAGTA
    TACGTTTATG ACATCTGCCA TGTGGTCATC GACAATCTGC AGTTCATGAT GGGGCATGAG
    CAGTTGTCCA CAGACAGGAT TGCAGCTCAA GACTACATCG TTGGGGCCTT TCGAAAGTTT
    GCAACAGACA ATAGCTGCCA TGTGACACTG GTCATTCATC CCCGGAAAGA GGATGATGAT
    AAGGAACTGC AGACAGCATC AATTTTTGGC TCAGCCAAAG CAAGCCAGGA AGCAGACAAT
    GTTCTGATCC TACAGGACAG GAAGCTGGTA ACTGGTCCAG GGAAACGGTA TCTGCAGGTG
    TCCAAGAACC GCTTTGATGG AGACGTAGGT GTCTTTCCAC TTGAATTCAA CAAGAGCTCT
    CTTACCTTCT CCATACCACC CAAGAGCAAG GCGCGGCTCA AGAAGGTCAA GGATGACAAC
    GGGCTTGTGA CCAAGAAGCC GTCCTCTGGC AAAAAAGGGG CTGTGCCCCA GAATTCTGAG
    ACTTGCTTGG GTCAGTCCCA CCACCCCTAT CAGCCAGACG TCTCCAAGCC CTCAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.