twnk-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	twnk-1
Entrez Gene ID	180845
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368519.1	NP_001355546.1	TWiNKle mtDNA helicase homolog
NM_001368518.3	NP_001355545.1	SF4 helicase domain-containing protein
NM_001368519.3	NP_001355546.1	SF4 helicase domain-containing protein
NM_001368520.3	NP_001355547.1	SF4 helicase domain-containing protein
NM_076572.4	NP_508973.3	TWiNKle mtDNA helicase homolog
NM_001368518.1	NP_001355545.1	TWiNKle mtDNA helicase homolog
NM_001368520.1	NP_001355547.1	TWiNKle mtDNA helicase homolog
NM_076572.6	NP_508973.3	SF4 helicase domain-containing protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peo1	NP_722491.2
Rattus norvegicus (Norway rat)	Peo1	NP_001101069.1
Drosophila melanogaster (fruit fly)	CG5924	NP_609318.1
Homo sapiens (human)	C10orf2	NP_068602.2
Pan troglodytes (chimpanzee)	C10H10orf2	XP_003951780.1
Canis lupus familiaris (dog)	C28H10orf2	XP_543974.1
Danio rerio (zebrafish)	peo1	NP_001264527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010720	XP_311434.3
Caenorhabditis elegans (roundworm)	F46G11.1	NP_508973.3
Gallus gallus (chicken)	PEO1	NP_001026515.1
Xenopus tropicalis (tropical clawed frog)	LOC100491229	XP_002938766.1
	C9H10orf2	XP_001109287.1
Bos taurus (cattle)	C26H10orf2	NP_001091933.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following twnk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twnk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa355362	NM_076572.6 Latest version!	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa698949	NM_001368518.3 Latest version!	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa698950	NM_001368519.3 Latest version!	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa698951	NM_001368520.3 Latest version!	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCa698950	NM_001368519.1	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa698949	NM_001368518.1	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa698951	NM_001368520.1	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa355362	NM_076572.4	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twnk-1 ( NM_076572.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508973.3 Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

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CloneID	OCa355362
Clone ID Related Accession (Same CDS sequence)	NM_076572.4 , NM_076572.6
Accession Version	NM_076572.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiNKle mtDNA helicase homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_076572.3. COMPLETENESS: incomplete on both ends.

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG GTATGATGAT 
CTAGTGTTTT TTCTATTTCC TTCACATCTT CGAAGCCTCG CGCGCACTAA CTCATATAAA 
AATGGCATCA AACTTCCGCT GCATCGAGTA GAGTATAGCA ACTCGATAAA TTCATGGTTG 
GATAGATTCG AGAGATCGAG TTCGGCGTTG ACAATGCTTG ACTCCGAAGA GTTCATGGAG 
AAGTCCATAA ATGAAATAGT CAGAGCAATT CGTATTCACG TTGAAAACAG TGGTATCCAA 
CATGTTGTCA TCGATAATCT CCAGTTTTTG ATTAACCAAG GAATGATGGC AGATGAGAAG 
TCAAGTGGCC TTGATAGATT CCACCTTCAG GATCGATTTG TCGGATACAT GCGTCAACTT 
GCTACTCAGA ATCAAATTCA CATCACTATG GTTGTTCATC CCAGAAAAAC TGACGGTGAT 
ACTGAAATTG ATGTGCAACA CTTTGGAGGT TCCGCCAGAG TCACCCAGGA AGCTGATAAT 
GTCATTGCTA TTCAGCGGAA GCGTGATGAT CGTGACCGTT CCAAGTTCCG AAAATTCTTA 
TACATTCTGA AAAACCGCTA CTATGGCCGA CGAGTGGAGA GCGATCAACT TGAGATGGTT 
TTCAACCCAT CCACCTATTC TCATACAGTT GTAGAGTTTC CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508973.3
CDS	1..1788
Translation	MEDELDSDLYDRVSYLNLMDKIDTRKKTSKVGKFRDDSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGYDDLVFFLFPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_076572.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076572.4

ORF Insert Sequence:

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG GTATGATGAT 
CTAGTGTTTT TTCTATTTCC TTCACATCTT CGAAGCCTCG CGCGCACTAA CTCATATAAA 
AATGGCATCA AACTTCCGCT GCATCGAGTA GAGTATAGCA ACTCGATAAA TTCATGGTTG 
GATAGATTCG AGAGATCGAG TTCGGCGTTG ACAATGCTTG ACTCCGAAGA GTTCATGGAG 
AAGTCCATAA ATGAAATAGT CAGAGCAATT CGTATTCACG TTGAAAACAG TGGTATCCAA 
CATGTTGTCA TCGATAATCT CCAGTTTTTG ATTAACCAAG GAATGATGGC AGATGAGAAG 
TCAAGTGGCC TTGATAGATT CCACCTTCAG GATCGATTTG TCGGATACAT GCGTCAACTT 
GCTACTCAGA ATCAAATTCA CATCACTATG GTTGTTCATC CCAGAAAAAC TGACGGTGAT 
ACTGAAATTG ATGTGCAACA CTTTGGAGGT TCCGCCAGAG TCACCCAGGA AGCTGATAAT 
GTCATTGCTA TTCAGCGGAA GCGTGATGAT CGTGACCGTT CCAAGTTCCG AAAATTCTTA 
TACATTCTGA AAAACCGCTA CTATGGCCGA CGAGTGGAGA GCGATCAACT TGAGATGGTT 
TTCAACCCAT CCACCTATTC TCATACAGTT GTAGAGTTTC CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_076572.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508973.3 Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Price & Availability↑

CloneID	OCa355362
Clone ID Related Accession (Same CDS sequence)	NM_076572.4 , NM_076572.6
Accession Version	NM_076572.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SF4 helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_076572.5.

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG GTATGATGAT 
CTAGTGTTTT TTCTATTTCC TTCACATCTT CGAAGCCTCG CGCGCACTAA CTCATATAAA 
AATGGCATCA AACTTCCGCT GCATCGAGTA GAGTATAGCA ACTCGATAAA TTCATGGTTG 
GATAGATTCG AGAGATCGAG TTCGGCGTTG ACAATGCTTG ACTCCGAAGA GTTCATGGAG 
AAGTCCATAA ATGAAATAGT CAGAGCAATT CGTATTCACG TTGAAAACAG TGGTATCCAA 
CATGTTGTCA TCGATAATCT CCAGTTTTTG ATTAACCAAG GAATGATGGC AGATGAGAAG 
TCAAGTGGCC TTGATAGATT CCACCTTCAG GATCGATTTG TCGGATACAT GCGTCAACTT 
GCTACTCAGA ATCAAATTCA CATCACTATG GTTGTTCATC CCAGAAAAAC TGACGGTGAT 
ACTGAAATTG ATGTGCAACA CTTTGGAGGT TCCGCCAGAG TCACCCAGGA AGCTGATAAT 
GTCATTGCTA TTCAGCGGAA GCGTGATGAT CGTGACCGTT CCAAGTTCCG AAAATTCTTA 
TACATTCTGA AAAACCGCTA CTATGGCCGA CGAGTGGAGA GCGATCAACT TGAGATGGTT 
TTCAACCCAT CCACCTATTC TCATACAGTT GTAGAGTTTC CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508973.3
CDS	42..1829
Translation	MEDELDSDLYDRVSYLNLMDKIDTRKKTSKVGKFRDDSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGYDDLVFFLFPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_076572.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076572.6

ORF Insert Sequence:

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG GTATGATGAT 
CTAGTGTTTT TTCTATTTCC TTCACATCTT CGAAGCCTCG CGCGCACTAA CTCATATAAA 
AATGGCATCA AACTTCCGCT GCATCGAGTA GAGTATAGCA ACTCGATAAA TTCATGGTTG 
GATAGATTCG AGAGATCGAG TTCGGCGTTG ACAATGCTTG ACTCCGAAGA GTTCATGGAG 
AAGTCCATAA ATGAAATAGT CAGAGCAATT CGTATTCACG TTGAAAACAG TGGTATCCAA 
CATGTTGTCA TCGATAATCT CCAGTTTTTG ATTAACCAAG GAATGATGGC AGATGAGAAG 
TCAAGTGGCC TTGATAGATT CCACCTTCAG GATCGATTTG TCGGATACAT GCGTCAACTT 
GCTACTCAGA ATCAAATTCA CATCACTATG GTTGTTCATC CCAGAAAAAC TGACGGTGAT 
ACTGAAATTG ATGTGCAACA CTTTGGAGGT TCCGCCAGAG TCACCCAGGA AGCTGATAAT 
GTCATTGCTA TTCAGCGGAA GCGTGATGAT CGTGACCGTT CCAAGTTCCG AAAATTCTTA 
TACATTCTGA AAAACCGCTA CTATGGCCGA CGAGTGGAGA GCGATCAACT TGAGATGGTT 
TTCAACCCAT CCACCTATTC TCATACAGTT GTAGAGTTTC CAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368518.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355545.1 Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Price & Availability↑

CloneID	OCa698949
Clone ID Related Accession (Same CDS sequence)	NM_001368518.3 , NM_001368518.1
Accession Version	NM_001368518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SF4 helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368518.2.

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG ACTTCCGCTG 
CATCGAGTAG AGTATAGCAA CTCGATAAAT TCATGGTTGG ATAGATTCGA GAGATCGAGT 
TCGGCGTTGA CAATGCTTGA CTCCGAAGAG TTCATGGAGA AGTCCATAAA TGAAATAGTC 
AGAGCAATTC GTATTCACGT TGAAAACAGT GGTATCCAAC ATGTTGTCAT CGATAATCTC 
CAGTTTTTGA TTAACCAAGG AATGATGGCA GATGAGAAGT CAAGTGGCCT TGATAGATTC 
CACCTTCAGG ATCGATTTGT CGGATACATG CGTCAACTTG CTACTCAGAA TCAAATTCAC 
ATCACTATGG TTGTTCATCC CAGAAAAACT GACGGTGATA CTGAAATTGA TGTGCAACAC 
TTTGGAGGTT CCGCCAGAGT CACCCAGGAA GCTGATAATG TCATTGCTAT TCAGCGGAAG 
CGTGATGATC GTGACCGTTC CAAGTTCCGA AAATTCTTAT ACATTCTGAA AAACCGCTAC 
TATGGCCGAC GAGTGGAGAG CGATCAACTT GAGATGGTTT TCAACCCATC CACCTATTCT 
CATACAGTTG TAGAGTTTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355545.1
CDS	42..1748
Translation	MEDELDSDLYDRVSYLNLMDKIDTRKKTSKVGKFRDDSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368518.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368518.3

ORF Insert Sequence:

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG ACTTCCGCTG 
CATCGAGTAG AGTATAGCAA CTCGATAAAT TCATGGTTGG ATAGATTCGA GAGATCGAGT 
TCGGCGTTGA CAATGCTTGA CTCCGAAGAG TTCATGGAGA AGTCCATAAA TGAAATAGTC 
AGAGCAATTC GTATTCACGT TGAAAACAGT GGTATCCAAC ATGTTGTCAT CGATAATCTC 
CAGTTTTTGA TTAACCAAGG AATGATGGCA GATGAGAAGT CAAGTGGCCT TGATAGATTC 
CACCTTCAGG ATCGATTTGT CGGATACATG CGTCAACTTG CTACTCAGAA TCAAATTCAC 
ATCACTATGG TTGTTCATCC CAGAAAAACT GACGGTGATA CTGAAATTGA TGTGCAACAC 
TTTGGAGGTT CCGCCAGAGT CACCCAGGAA GCTGATAATG TCATTGCTAT TCAGCGGAAG 
CGTGATGATC GTGACCGTTC CAAGTTCCGA AAATTCTTAT ACATTCTGAA AAACCGCTAC 
TATGGCCGAC GAGTGGAGAG CGATCAACTT GAGATGGTTT TCAACCCATC CACCTATTCT 
CATACAGTTG TAGAGTTTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368518.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355545.1 Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

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CloneID	OCa698949
Clone ID Related Accession (Same CDS sequence)	NM_001368518.3 , NM_001368518.1
Accession Version	NM_001368518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiNKle mtDNA helicase homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG ACTTCCGCTG 
CATCGAGTAG AGTATAGCAA CTCGATAAAT TCATGGTTGG ATAGATTCGA GAGATCGAGT 
TCGGCGTTGA CAATGCTTGA CTCCGAAGAG TTCATGGAGA AGTCCATAAA TGAAATAGTC 
AGAGCAATTC GTATTCACGT TGAAAACAGT GGTATCCAAC ATGTTGTCAT CGATAATCTC 
CAGTTTTTGA TTAACCAAGG AATGATGGCA GATGAGAAGT CAAGTGGCCT TGATAGATTC 
CACCTTCAGG ATCGATTTGT CGGATACATG CGTCAACTTG CTACTCAGAA TCAAATTCAC 
ATCACTATGG TTGTTCATCC CAGAAAAACT GACGGTGATA CTGAAATTGA TGTGCAACAC 
TTTGGAGGTT CCGCCAGAGT CACCCAGGAA GCTGATAATG TCATTGCTAT TCAGCGGAAG 
CGTGATGATC GTGACCGTTC CAAGTTCCGA AAATTCTTAT ACATTCTGAA AAACCGCTAC 
TATGGCCGAC GAGTGGAGAG CGATCAACTT GAGATGGTTT TCAACCCATC CACCTATTCT 
CATACAGTTG TAGAGTTTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355545.1
CDS	1..1707
Translation	MEDELDSDLYDRVSYLNLMDKIDTRKKTSKVGKFRDDSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368518.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368518.1

ORF Insert Sequence:

ATGGAAGACG AACTCGACTC GGATCTCTAC GATCGTGTGA GCTATTTGAA TTTGATGGAC 
AAAATCGATA CTCGGAAGAA GACATCGAAA GTGGGAAAGT TCCGCGATGA TTCTGACGCG 
CCTTCTCCAA GACTTGACGC TTCAGAAGCC ATGAGTGCTC CTCCAGATCC GGATATCAAG 
CGCTACTGGG ACAGCAGTTT AGACATAACT GATCTGAGTG GCCACGATTT CACTATTTTC 
AAGCAACTCC AAGGGATGCT TGGAATTGAG AACATTTCAT TCGAAACTCT TGCTCGTTTC 
AACGTTCGTG TCCAAACCGA CACCTATGGT CAACCTGCCA TTCTTTTTCC ACGGTATCGT 
GGAATGACTT CCCGGCTACG CATTCCAATG GGTTTGAAGT TGATCCGTAA AGTTGGAGAC 
AGAATGGAAA AGGAGAACTA TCCACTGCCA GATGAAACTT CCGTTCGCCA AAAGTTCAAT 
GGAGTTTTCG GGCTTCATAT GGCAACTCTG TCAGATCGAA GTGTAGTCAT TACCACAAAT 
GAGCGTGATG CTTTGGCGAT CTACGAAGCC ACCAAAGCTC TGGTATTTGC TCTACCACAT 
GGAGAAATTT TGGATCCACT CGTGCTTCCG TATCTGGAGG AATTTGACAA GATCTATCTG 
TGGTTCCCGG TTCAGCATGT CAGTTATGCC AAAGACTGGG GAAGTGCTTT GAACACCCTC 
AAATGTCTTC TTGTGAAAAA CGAAGAACGC CCAATTGAAT TGGTTCGTAA TGGTGATCAT 
CGGCTCATTC GGAACTCACT ATCTAACTCG GTGGTTCGAA TGAGAGAGCG TGGTTTCCGT 
TCAATGACAG ATTTAGACAT GCGTGAAGGA ATCCGTTCAG ACCTTCTCAA CTCGACAAAT 
CGCGTTGTTG GATTTGGACA ATGGAAACGA TTTGCTGTTT TGAATAAGTA TCTTGGCGGG 
CTTCGGCCAG GGGAGATGAC AGTTTTAACC GGAGGAACCG GCAACGGAAA AACTACATTT 
TTATGTGAAT ACTCATTGGA TCTATTTACT CAAGGGGTGC GCACGCTGTT CTGCAGTTTC 
GAGATGCCTG AAAAGAAGAT CCTCCATTGG ATGCTTGTGC AATACGCTGG ACTTCCGCTG 
CATCGAGTAG AGTATAGCAA CTCGATAAAT TCATGGTTGG ATAGATTCGA GAGATCGAGT 
TCGGCGTTGA CAATGCTTGA CTCCGAAGAG TTCATGGAGA AGTCCATAAA TGAAATAGTC 
AGAGCAATTC GTATTCACGT TGAAAACAGT GGTATCCAAC ATGTTGTCAT CGATAATCTC 
CAGTTTTTGA TTAACCAAGG AATGATGGCA GATGAGAAGT CAAGTGGCCT TGATAGATTC 
CACCTTCAGG ATCGATTTGT CGGATACATG CGTCAACTTG CTACTCAGAA TCAAATTCAC 
ATCACTATGG TTGTTCATCC CAGAAAAACT GACGGTGATA CTGAAATTGA TGTGCAACAC 
TTTGGAGGTT CCGCCAGAGT CACCCAGGAA GCTGATAATG TCATTGCTAT TCAGCGGAAG 
CGTGATGATC GTGACCGTTC CAAGTTCCGA AAATTCTTAT ACATTCTGAA AAACCGCTAC 
TATGGCCGAC GAGTGGAGAG CGATCAACTT GAGATGGTTT TCAACCCATC CACCTATTCT 
CATACAGTTG TAGAGTTTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368519.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355546.1 Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

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CloneID	OCa698950
Clone ID Related Accession (Same CDS sequence)	NM_001368519.1 , NM_001368519.3
Accession Version	NM_001368519.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiNKle mtDNA helicase homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGTCCTTC ACATCTTCGA 
AGCCTCGCGC GCACTAACTC ATATAAAAAT GGCATCAAAC TTCCGCTGCA TCGAGTAGAG 
TATAGCAACT CGATAAATTC ATGGTTGGAT AGATTCGAGA GATCGAGTTC GGCGTTGACA 
ATGCTTGACT CCGAAGAGTT CATGGAGAAG TCCATAAATG AAATAGTCAG AGCAATTCGT 
ATTCACGTTG AAAACAGTGG TATCCAACAT GTTGTCATCG ATAATCTCCA GTTTTTGATT 
AACCAAGGAA TGATGGCAGA TGAGAAGTCA AGTGGCCTTG ATAGATTCCA CCTTCAGGAT 
CGATTTGTCG GATACATGCG TCAACTTGCT ACTCAGAATC AAATTCACAT CACTATGGTT 
GTTCATCCCA GAAAAACTGA CGGTGATACT GAAATTGATG TGCAACACTT TGGAGGTTCC 
GCCAGAGTCA CCCAGGAAGC TGATAATGTC ATTGCTATTC AGCGGAAGCG TGATGATCGT 
GACCGTTCCA AGTTCCGAAA ATTCTTATAC ATTCTGAAAA ACCGCTACTA TGGCCGACGA 
GTGGAGAGCG ATCAACTTGA GATGGTTTTC AACCCATCCA CCTATTCTCA TACAGTTGTA 
GAGTTTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355546.1
CDS	1..1815
Translation	MDDYTILPSPDEAPFDHSDDEYFTGRRLRQRKAPRTGRSVTASESMNQIADFDFSSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368519.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368519.1

ORF Insert Sequence:

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGTCCTTC ACATCTTCGA 
AGCCTCGCGC GCACTAACTC ATATAAAAAT GGCATCAAAC TTCCGCTGCA TCGAGTAGAG 
TATAGCAACT CGATAAATTC ATGGTTGGAT AGATTCGAGA GATCGAGTTC GGCGTTGACA 
ATGCTTGACT CCGAAGAGTT CATGGAGAAG TCCATAAATG AAATAGTCAG AGCAATTCGT 
ATTCACGTTG AAAACAGTGG TATCCAACAT GTTGTCATCG ATAATCTCCA GTTTTTGATT 
AACCAAGGAA TGATGGCAGA TGAGAAGTCA AGTGGCCTTG ATAGATTCCA CCTTCAGGAT 
CGATTTGTCG GATACATGCG TCAACTTGCT ACTCAGAATC AAATTCACAT CACTATGGTT 
GTTCATCCCA GAAAAACTGA CGGTGATACT GAAATTGATG TGCAACACTT TGGAGGTTCC 
GCCAGAGTCA CCCAGGAAGC TGATAATGTC ATTGCTATTC AGCGGAAGCG TGATGATCGT 
GACCGTTCCA AGTTCCGAAA ATTCTTATAC ATTCTGAAAA ACCGCTACTA TGGCCGACGA 
GTGGAGAGCG ATCAACTTGA GATGGTTTTC AACCCATCCA CCTATTCTCA TACAGTTGTA 
GAGTTTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368519.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355546.1 Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Price & Availability↑

CloneID	OCa698950
Clone ID Related Accession (Same CDS sequence)	NM_001368519.1 , NM_001368519.3
Accession Version	NM_001368519.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SF4 helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368519.2.

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGTCCTTC ACATCTTCGA 
AGCCTCGCGC GCACTAACTC ATATAAAAAT GGCATCAAAC TTCCGCTGCA TCGAGTAGAG 
TATAGCAACT CGATAAATTC ATGGTTGGAT AGATTCGAGA GATCGAGTTC GGCGTTGACA 
ATGCTTGACT CCGAAGAGTT CATGGAGAAG TCCATAAATG AAATAGTCAG AGCAATTCGT 
ATTCACGTTG AAAACAGTGG TATCCAACAT GTTGTCATCG ATAATCTCCA GTTTTTGATT 
AACCAAGGAA TGATGGCAGA TGAGAAGTCA AGTGGCCTTG ATAGATTCCA CCTTCAGGAT 
CGATTTGTCG GATACATGCG TCAACTTGCT ACTCAGAATC AAATTCACAT CACTATGGTT 
GTTCATCCCA GAAAAACTGA CGGTGATACT GAAATTGATG TGCAACACTT TGGAGGTTCC 
GCCAGAGTCA CCCAGGAAGC TGATAATGTC ATTGCTATTC AGCGGAAGCG TGATGATCGT 
GACCGTTCCA AGTTCCGAAA ATTCTTATAC ATTCTGAAAA ACCGCTACTA TGGCCGACGA 
GTGGAGAGCG ATCAACTTGA GATGGTTTTC AACCCATCCA CCTATTCTCA TACAGTTGTA 
GAGTTTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355546.1
CDS	327..2141
Translation	MDDYTILPSPDEAPFDHSDDEYFTGRRLRQRKAPRTGRSVTASESMNQIADFDFSSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGPSHLRSLARTNSYKNGIKLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368519.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368519.3

ORF Insert Sequence:

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGTCCTTC ACATCTTCGA 
AGCCTCGCGC GCACTAACTC ATATAAAAAT GGCATCAAAC TTCCGCTGCA TCGAGTAGAG 
TATAGCAACT CGATAAATTC ATGGTTGGAT AGATTCGAGA GATCGAGTTC GGCGTTGACA 
ATGCTTGACT CCGAAGAGTT CATGGAGAAG TCCATAAATG AAATAGTCAG AGCAATTCGT 
ATTCACGTTG AAAACAGTGG TATCCAACAT GTTGTCATCG ATAATCTCCA GTTTTTGATT 
AACCAAGGAA TGATGGCAGA TGAGAAGTCA AGTGGCCTTG ATAGATTCCA CCTTCAGGAT 
CGATTTGTCG GATACATGCG TCAACTTGCT ACTCAGAATC AAATTCACAT CACTATGGTT 
GTTCATCCCA GAAAAACTGA CGGTGATACT GAAATTGATG TGCAACACTT TGGAGGTTCC 
GCCAGAGTCA CCCAGGAAGC TGATAATGTC ATTGCTATTC AGCGGAAGCG TGATGATCGT 
GACCGTTCCA AGTTCCGAAA ATTCTTATAC ATTCTGAAAA ACCGCTACTA TGGCCGACGA 
GTGGAGAGCG ATCAACTTGA GATGGTTTTC AACCCATCCA CCTATTCTCA TACAGTTGTA 
GAGTTTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368520.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355547.1 Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

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CloneID	OCa698951
Clone ID Related Accession (Same CDS sequence)	NM_001368520.3 , NM_001368520.1
Accession Version	NM_001368520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SF4 helicase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368520.2.

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGACTTCC GCTGCATCGA 
GTAGAGTATA GCAACTCGAT AAATTCATGG TTGGATAGAT TCGAGAGATC GAGTTCGGCG 
TTGACAATGC TTGACTCCGA AGAGTTCATG GAGAAGTCCA TAAATGAAAT AGTCAGAGCA 
ATTCGTATTC ACGTTGAAAA CAGTGGTATC CAACATGTTG TCATCGATAA TCTCCAGTTT 
TTGATTAACC AAGGAATGAT GGCAGATGAG AAGTCAAGTG GCCTTGATAG ATTCCACCTT 
CAGGATCGAT TTGTCGGATA CATGCGTCAA CTTGCTACTC AGAATCAAAT TCACATCACT 
ATGGTTGTTC ATCCCAGAAA AACTGACGGT GATACTGAAA TTGATGTGCA ACACTTTGGA 
GGTTCCGCCA GAGTCACCCA GGAAGCTGAT AATGTCATTG CTATTCAGCG GAAGCGTGAT 
GATCGTGACC GTTCCAAGTT CCGAAAATTC TTATACATTC TGAAAAACCG CTACTATGGC 
CGACGAGTGG AGAGCGATCA ACTTGAGATG GTTTTCAACC CATCCACCTA TTCTCATACA 
GTTGTAGAGT TTCCAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355547.1
CDS	327..2087
Translation	MDDYTILPSPDEAPFDHSDDEYFTGRRLRQRKAPRTGRSVTASESMNQIADFDFSSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368520.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SF4 helicase domain-containing protein (twnk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368520.3

ORF Insert Sequence:

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGACTTCC GCTGCATCGA 
GTAGAGTATA GCAACTCGAT AAATTCATGG TTGGATAGAT TCGAGAGATC GAGTTCGGCG 
TTGACAATGC TTGACTCCGA AGAGTTCATG GAGAAGTCCA TAAATGAAAT AGTCAGAGCA 
ATTCGTATTC ACGTTGAAAA CAGTGGTATC CAACATGTTG TCATCGATAA TCTCCAGTTT 
TTGATTAACC AAGGAATGAT GGCAGATGAG AAGTCAAGTG GCCTTGATAG ATTCCACCTT 
CAGGATCGAT TTGTCGGATA CATGCGTCAA CTTGCTACTC AGAATCAAAT TCACATCACT 
ATGGTTGTTC ATCCCAGAAA AACTGACGGT GATACTGAAA TTGATGTGCA ACACTTTGGA 
GGTTCCGCCA GAGTCACCCA GGAAGCTGAT AATGTCATTG CTATTCAGCG GAAGCGTGAT 
GATCGTGACC GTTCCAAGTT CCGAAAATTC TTATACATTC TGAAAAACCG CTACTATGGC 
CGACGAGTGG AGAGCGATCA ACTTGAGATG GTTTTCAACC CATCCACCTA TTCTCATACA 
GTTGTAGAGT TTCCAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twnk-1 ( NM_001368520.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355547.1 Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

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CloneID	OCa698951
Clone ID Related Accession (Same CDS sequence)	NM_001368520.3 , NM_001368520.1
Accession Version	NM_001368520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiNKle mtDNA helicase homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGACTTCC GCTGCATCGA 
GTAGAGTATA GCAACTCGAT AAATTCATGG TTGGATAGAT TCGAGAGATC GAGTTCGGCG 
TTGACAATGC TTGACTCCGA AGAGTTCATG GAGAAGTCCA TAAATGAAAT AGTCAGAGCA 
ATTCGTATTC ACGTTGAAAA CAGTGGTATC CAACATGTTG TCATCGATAA TCTCCAGTTT 
TTGATTAACC AAGGAATGAT GGCAGATGAG AAGTCAAGTG GCCTTGATAG ATTCCACCTT 
CAGGATCGAT TTGTCGGATA CATGCGTCAA CTTGCTACTC AGAATCAAAT TCACATCACT 
ATGGTTGTTC ATCCCAGAAA AACTGACGGT GATACTGAAA TTGATGTGCA ACACTTTGGA 
GGTTCCGCCA GAGTCACCCA GGAAGCTGAT AATGTCATTG CTATTCAGCG GAAGCGTGAT 
GATCGTGACC GTTCCAAGTT CCGAAAATTC TTATACATTC TGAAAAACCG CTACTATGGC 
CGACGAGTGG AGAGCGATCA ACTTGAGATG GTTTTCAACC CATCCACCTA TTCTCATACA 
GTTGTAGAGT TTCCAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355547.1
CDS	1..1761
Translation	MDDYTILPSPDEAPFDHSDDEYFTGRRLRQRKAPRTGRSVTASESMNQIADFDFSSDAPSPRLDASEAMSAPPDPDIKRYWDSSLDITDLSGHDFTIFKQLQGMLGIENISFETLARFNVRVQTDTYGQPAILFPRYRGMTSRLRIPMGLKLIRKVGDRMEKENYPLPDETSVRQKFNGVFGLHMATLSDRSVVITTNERDALAIYEATKALVFALPHGEILDPLVLPYLEEFDKIYLWFPVQHVSYAKDWGSALNTLKCLLVKNEERPIELVRNGDHRLIRNSLSNSVVRMRERGFRSMTDLDMREGIRSDLLNSTNRVVGFGQWKRFAVLNKYLGGLRPGEMTVLTGGTGNGKTTFLCEYSLDLFTQGVRTLFCSFEMPEKKILHWMLVQYAGLPLHRVEYSNSINSWLDRFERSSSALTMLDSEEFMEKSINEIVRAIRIHVENSGIQHVVIDNLQFLINQGMMADEKSSGLDRFHLQDRFVGYMRQLATQNQIHITMVVHPRKTDGDTEIDVQHFGGSARVTQEADNVIAIQRKRDDRDRSKFRKFLYILKNRYYGRRVESDQLEMVFNPSTYSHTVVEFPK

Target ORF information:

RefSeq Version	NM_001368520.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiNKle mtDNA helicase homolog (twnk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368520.1

ORF Insert Sequence:

ATGGATGACT ATACAATTCT GCCAAGCCCC GACGAAGCTC CATTTGATCA CAGCGACGAC 
GAATATTTCA CCGGAAGACG ACTACGGCAG AGAAAAGCAC CCCGGACGGG AAGATCGGTG 
ACAGCGTCTG AATCGATGAA TCAGATTGCA GACTTTGATT TCAGTTCTGA CGCGCCTTCT 
CCAAGACTTG ACGCTTCAGA AGCCATGAGT GCTCCTCCAG ATCCGGATAT CAAGCGCTAC 
TGGGACAGCA GTTTAGACAT AACTGATCTG AGTGGCCACG ATTTCACTAT TTTCAAGCAA 
CTCCAAGGGA TGCTTGGAAT TGAGAACATT TCATTCGAAA CTCTTGCTCG TTTCAACGTT 
CGTGTCCAAA CCGACACCTA TGGTCAACCT GCCATTCTTT TTCCACGGTA TCGTGGAATG 
ACTTCCCGGC TACGCATTCC AATGGGTTTG AAGTTGATCC GTAAAGTTGG AGACAGAATG 
GAAAAGGAGA ACTATCCACT GCCAGATGAA ACTTCCGTTC GCCAAAAGTT CAATGGAGTT 
TTCGGGCTTC ATATGGCAAC TCTGTCAGAT CGAAGTGTAG TCATTACCAC AAATGAGCGT 
GATGCTTTGG CGATCTACGA AGCCACCAAA GCTCTGGTAT TTGCTCTACC ACATGGAGAA 
ATTTTGGATC CACTCGTGCT TCCGTATCTG GAGGAATTTG ACAAGATCTA TCTGTGGTTC 
CCGGTTCAGC ATGTCAGTTA TGCCAAAGAC TGGGGAAGTG CTTTGAACAC CCTCAAATGT 
CTTCTTGTGA AAAACGAAGA ACGCCCAATT GAATTGGTTC GTAATGGTGA TCATCGGCTC 
ATTCGGAACT CACTATCTAA CTCGGTGGTT CGAATGAGAG AGCGTGGTTT CCGTTCAATG 
ACAGATTTAG ACATGCGTGA AGGAATCCGT TCAGACCTTC TCAACTCGAC AAATCGCGTT 
GTTGGATTTG GACAATGGAA ACGATTTGCT GTTTTGAATA AGTATCTTGG CGGGCTTCGG 
CCAGGGGAGA TGACAGTTTT AACCGGAGGA ACCGGCAACG GAAAAACTAC ATTTTTATGT 
GAATACTCAT TGGATCTATT TACTCAAGGG GTGCGCACGC TGTTCTGCAG TTTCGAGATG 
CCTGAAAAGA AGATCCTCCA TTGGATGCTT GTGCAATACG CTGGACTTCC GCTGCATCGA 
GTAGAGTATA GCAACTCGAT AAATTCATGG TTGGATAGAT TCGAGAGATC GAGTTCGGCG 
TTGACAATGC TTGACTCCGA AGAGTTCATG GAGAAGTCCA TAAATGAAAT AGTCAGAGCA 
ATTCGTATTC ACGTTGAAAA CAGTGGTATC CAACATGTTG TCATCGATAA TCTCCAGTTT 
TTGATTAACC AAGGAATGAT GGCAGATGAG AAGTCAAGTG GCCTTGATAG ATTCCACCTT 
CAGGATCGAT TTGTCGGATA CATGCGTCAA CTTGCTACTC AGAATCAAAT TCACATCACT 
ATGGTTGTTC ATCCCAGAAA AACTGACGGT GATACTGAAA TTGATGTGCA ACACTTTGGA 
GGTTCCGCCA GAGTCACCCA GGAAGCTGAT AATGTCATTG CTATTCAGCG GAAGCGTGAT 
GATCGTGACC GTTCCAAGTT CCGAAAATTC TTATACATTC TGAAAAACCG CTACTATGGC 
CGACGAGTGG AGAGCGATCA ACTTGAGATG GTTTTCAACC CATCCACCTA TTCTCATACA 
GTTGTAGAGT TTCCAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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twnk-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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