TWNK gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001368275.1	NP_001355204.1	twinkle protein, mitochondrial isoform C
XM_011539975.2	XP_011538277.1	twinkle protein, mitochondrial isoform X1
NM_001163812.2	NP_001157284.1	twinkle protein, mitochondrial isoform B
NM_021830.5	NP_068602.2	twinkle protein, mitochondrial isoform A
NM_001163813.2	NP_001157285.1	twinkle protein, mitochondrial isoform C
XM_017016437.1	XP_016871926.1	twinkle protein, mitochondrial isoform X1
NM_001163813.1	NP_001157285.1	twinkle protein, mitochondrial isoform C
NM_001163814.2	NP_001157286.1	twinkle protein, mitochondrial isoform D
NM_001163814.1	NP_001157286.1	twinkle protein, mitochondrial isoform D
NM_001163812.1	NP_001157284.1	twinkle protein, mitochondrial isoform B
NM_021830.4	NP_068602.2	twinkle protein, mitochondrial isoform A

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26782	NM_021830.5 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
OHu26432	NM_001368275.1 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu26432	NM_001163813.2 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu26432	XM_011539975.2 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu26432	XM_017016437.1 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu22794	NM_001163814.2 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu26964	NM_001163812.2 Latest version!	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OHu26432	NM_001163813.1	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu22794	NM_001163814.1	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu26964	NM_001163812.1	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OHu26782	NM_021830.4	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
Hide Old Accession Versions >>

TWNK ( NM_021830.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_068602.2 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26782
Clone ID Related Accession (Same CDS sequence)	NM_021830.5 , NM_021830.4
Accession Version	NM_021830.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090322.1, AF292004.1, AK025485.1 and AL133215.17. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_021830.4. Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.153796.1, SRR1660807.228176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000311916.8/ ENSP00000309595.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC 
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA 
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG 
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG 
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG 
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC 
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068602.2
CDS	669..2723
Translation	MWVLLRSGYPLRILLPLRGEWMGRRGLPRNLAPGPPRRRYRKETLQALDMPVLPVTATEIRQYLRGHGIPFQDGHSCLRALSPFAESSQLKGQTGVTTSFSLFIDKTTGHFLCMTSLAEGSWEDFQASVEGRGDGAREGFLLSKAPEFEDSEEVRRIWNRAIPLWELPDQEEVQLADTMFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	NM_021830.5
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021830.5

ORF Insert Sequence:

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC 
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA 
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG 
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG 
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG 
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC 
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_021830.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068602.2 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26782
Clone ID Related Accession (Same CDS sequence)	NM_021830.5 , NM_021830.4
Accession Version	NM_021830.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292004.1 and AK025485.1. This sequence is a reference standard in the RefSeqGene project. On Aug 5, 2009 this sequence version replaced NM_021830.3. Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033762.1, SRR1660803.153796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC 
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA 
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG 
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG 
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG 
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC 
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068602.2
CDS	676..2730
Translation	MWVLLRSGYPLRILLPLRGEWMGRRGLPRNLAPGPPRRRYRKETLQALDMPVLPVTATEIRQYLRGHGIPFQDGHSCLRALSPFAESSQLKGQTGVTTSFSLFIDKTTGHFLCMTSLAEGSWEDFQASVEGRGDGAREGFLLSKAPEFEDSEEVRRIWNRAIPLWELPDQEEVQLADTMFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	NM_021830.4
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021830.4

ORF Insert Sequence:

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC 
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA 
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG 
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG 
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG 
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC 
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001368275.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001355204.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu26432
Clone ID Related Accession (Same CDS sequence)	NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
Accession Version	NM_001368275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133215.17. On Feb 2, 2019 this sequence version replaced XM_011539975.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.431917.1, SRR1163657.209190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355204.1
CDS	129..821
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	NM_001368275.1
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368275.1

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163813.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157285.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26432
Clone ID Related Accession (Same CDS sequence)	NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
Accession Version	NM_001163813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, BQ643363.1, AK025485.1 and AL133215.17. On May 31, 2019 this sequence version replaced NM_001163813.1. Transcript Variant: This variant (3) uses an alternate splice site in exon 1 that results in the use of an in-frame downstream start codon, compared to variant 1. The encoded protein (isoform C) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297068.1, SRR1803611.169698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157285.1
CDS	191..883
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	NM_001163813.2
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163813.2

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163813.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157285.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26432
Clone ID Related Accession (Same CDS sequence)	NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
Accession Version	NM_001163813.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, BQ643363.1 and AK025485.1. Transcript Variant: This variant (3) uses an alternate splice site in exon 1 that results in the use of an in-frame downstream start codon, compared to variant 1. The encoded protein (isoform C) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.349144.1, SRR1803611.169698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157285.1
CDS	198..890
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	NM_001163813.1
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163813.1

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( XM_011539975.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538277.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu26432
Clone ID Related Accession (Same CDS sequence)	NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
Accession Version	XM_011539975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538277.1
CDS	145..837
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	XM_011539975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539975.2

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( XM_017016437.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871926.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu26432
Clone ID Related Accession (Same CDS sequence)	NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
Accession Version	XM_017016437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871926.1
CDS	1475..2167
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK

Target ORF information:

RefSeq Version	XM_017016437.1
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016437.1

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG 
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA 
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC 
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT 
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC 
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163814.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157286.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22794
Clone ID Related Accession (Same CDS sequence)	NM_001163814.2 , NM_001163814.1
Accession Version	NM_001163814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, AL133215.17 and AK025485.1. On May 31, 2019 this sequence version replaced NM_001163814.1. Transcript Variant: This variant (4) has multiple splice site differences, compared to variant 1. The encoded protein (isoform D) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, and has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.192749.1, AL529767.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157286.1
CDS	191..577
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVSGL

Target ORF information:

RefSeq Version	NM_001163814.2
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163814.2

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163814.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157286.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22794
Clone ID Related Accession (Same CDS sequence)	NM_001163814.2 , NM_001163814.1
Accession Version	NM_001163814.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, AL133215.17 and AK025485.1. Transcript Variant: This variant (4) has multiple splice site differences, compared to variant 1. The encoded protein (isoform D) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, and has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL529767.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157286.1
CDS	198..584
Translation	MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVSGL

Target ORF information:

RefSeq Version	NM_001163814.1
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163814.1

ORF Insert Sequence:

ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC 
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC 
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT 
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA 
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT 
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163812.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157284.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26964
Clone ID Related Accession (Same CDS sequence)	NM_001163812.2 , NM_001163812.1
Accession Version	NM_001163812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090322.1, AF292005.1, BC013349.2, AK025485.1 and AL133215.17. On May 31, 2019 this sequence version replaced NM_001163812.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region which introduces a novel stop codon, compared to variant 1. The encoded protein (isoform B; also known as Twinky) has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1714090.1, SRR1803616.64312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT 
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157284.1
CDS	669..2417
Translation	MWVLLRSGYPLRILLPLRGEWMGRRGLPRNLAPGPPRRRYRKETLQALDMPVLPVTATEIRQYLRGHGIPFQDGHSCLRALSPFAESSQLKGQTGVTTSFSLFIDKTTGHFLCMTSLAEGSWEDFQASVEGRGDGAREGFLLSKAPEFEDSEEVRRIWNRAIPLWELPDQEEVQLADTMFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVSGL

Target ORF information:

RefSeq Version	NM_001163812.2
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163812.2

ORF Insert Sequence:

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT 
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWNK ( NM_001163812.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157284.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26964
Clone ID Related Accession (Same CDS sequence)	NM_001163812.2 , NM_001163812.1
Accession Version	NM_001163812.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	twinkle protein, mitochondrial isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292005.1, BC013349.2 and AK025485.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region which introduces a novel stop codon, compared to variant 1. The encoded protein (isoform B; also known as Twinky) has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.12391.1, SRR1803616.64312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT 
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157284.1
CDS	676..2424
Translation	MWVLLRSGYPLRILLPLRGEWMGRRGLPRNLAPGPPRRRYRKETLQALDMPVLPVTATEIRQYLRGHGIPFQDGHSCLRALSPFAESSQLKGQTGVTTSFSLFIDKTTGHFLCMTSLAEGSWEDFQASVEGRGDGAREGFLLSKAPEFEDSEEVRRIWNRAIPLWELPDQEEVQLADTMFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKVSGL

Target ORF information:

RefSeq Version	NM_001163812.1
Organism	Homo sapiens(human)
Definition	Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163812.1

ORF Insert Sequence:

ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC 
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC 
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA 
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC 
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG 
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT 
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA 
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT 
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT 
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG 
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA 
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG 
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC 
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG 
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG 
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC 
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG 
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA 
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT 
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA 
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG 
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC 
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC 
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA 
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC 
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG 
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG 
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT 
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TWNK
Entrez Gene ID	56652
Full Name	twinkle mtDNA helicase
Synonyms	ATXN8,C10orf2,IOSCA,MTDPS7,PEO,PEO1,PEOA3,PRLTS5,SANDO,SCA8,TWINL
General protein information	Preferred Names twinkle mtDNA helicase Names twinkle protein, mitochondrial T7 gp4-like protein with intramitochondrial nucleoid localization T7 helicase-related protein with intramitochondrial nucleoid localization T7-like mitochondrial DNA helicase ataxin 8 mitochondrial twinkle protein progressive external ophthalmoplegia 1 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q24.31
Summary	This gene encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Aug 2009].
Disorder MIM:	606075
Disorder Html:	Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 3, 609286 (3); Mitochondrial DNA depletion syndrome 7 (hepatocerebral type), 271245 (3); Perrault syndrome 5, 616138 (3)

Mus musculus (house mouse)	Peo1	NP_722491.2
Rattus norvegicus (Norway rat)	Peo1	NP_001101069.1
Drosophila melanogaster (fruit fly)	CG5924	NP_609318.1
Homo sapiens (human)	C10orf2	NP_068602.2
Pan troglodytes (chimpanzee)	C10H10orf2	XP_003951780.1
Canis lupus familiaris (dog)	C28H10orf2	XP_543974.1
Danio rerio (zebrafish)	peo1	NP_001264527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010720	XP_311434.3
Caenorhabditis elegans (roundworm)	F46G11.1	NP_508973.3
Gallus gallus (chicken)	PEO1	NP_001026515.1
Xenopus tropicalis (tropical clawed frog)	LOC100491229	XP_002938766.1
	C9H10orf2	XP_001109287.1
Bos taurus (cattle)	C26H10orf2	NP_001091933.1

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TWNK cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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