Fim cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fim
Entrez Gene ID	32721
Full Name	Fimbrin
Synonyms	CG8649,Dmel\CG8649,FIM,fim
General protein information	Preferred Names Fimbrin Names CG8649 gene product from transcript CG8649-RA CG8649-PA CG8649-PC CG8649-PD Fim-PA Fim-PC Fim-PD fimbrin plastin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15F8-16A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078661.4	NP_523385.1	fimbrin, isoform A
NM_167564.2	NP_728073.1	fimbrin, isoform C
NM_167565.2	NP_728074.1	fimbrin, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004071	XP_309626.4
Kluyveromyces lactis	KLLA0F19778g	XP_455968.1
Magnaporthe oryzae (rice blast fungus)	MGG_04478	XP_003710980.1
Arabidopsis thaliana (thale cress)	FIM2	NP_198420.1
Oryza sativa (rice)	Os02g0588300	NP_001047274.1
Homo sapiens (human)	LCP1	NP_002289.2
Pan troglodytes (chimpanzee)	LCP1	XP_001158161.1
Rattus norvegicus (Norway rat)	Lcp1	NP_001012044.1
Gallus gallus (chicken)	LCP1	NP_001008440.1
Drosophila melanogaster (fruit fly)	Fim	NP_523385.1
Caenorhabditis elegans (roundworm)	plst-1	NP_500061.4
Saccharomyces cerevisiae (baker's yeast)	SAC6	NP_010414.3
Schizosaccharomyces pombe (fission yeast)	fim1	NP_596289.1
	LCP1	XP_002800787.1
Canis lupus familiaris (dog)	LCP1	XP_005633943.1
Mus musculus (house mouse)	Lcp1	NP_032905.2
Danio rerio (zebrafish)	lcp1	NP_571395.1
Arabidopsis thaliana (thale cress)	AT2G04750	NP_178552.1
Xenopus tropicalis (tropical clawed frog)	lcp1	NP_001015721.1
Bos taurus (cattle)	LCP1	NP_001029892.1
Eremothecium gossypii	AGOS_AGR069C	NP_986734.2
Neurospora crassa	NCU03992	XP_956577.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01897	NM_078661.4 Latest version!	Drosophila melanogaster fimbrin, transcript variant A (Fim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01898	NM_167564.2 Latest version!	Drosophila melanogaster fimbrin, transcript variant C (Fim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01899	NM_167565.2 Latest version!	Drosophila melanogaster fimbrin, transcript variant D (Fim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fim ( NM_078661.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523385.1 Drosophila melanogaster fimbrin, transcript variant A (Fim), mRNA.

Price & Availability↑

CloneID	OFa01897
Clone ID Related Accession (Same CDS sequence)	NM_078661.4
Accession Version	NM_078661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fimbrin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078661.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACAC TTAACAAATT CACAAAAACG CTGTCCATTG ATGAAAAGGC GGAGATCAAG 
GAGAAATTCA TAGAGTTGGA TGCCAACAAA GATGGCTTCA TCGATCTGCA CGAGCTAAAG 
GATGCCCTCA ACCAGGTGGG CTTCAAGCTG GCCGGCTACC AGGTGCGCGA AATGATTGAC 
GAGTACAAGG GCAAACAGCT GACTGCCTTC CAGGGCAAAT TGAATCTGGA GGAGTTCGAG 
GCACTGTGCC TGGACCTGAA GAGCAAGGAT GTGGCCAGCA CATTCAAGAC GGTCGTCTCC 
AAGAAGGAGA ACTTGGAGAC CCTGGGCGGC ATGTCGAGCA TTTCGTCGGA GGGCACCACC 
CATTCGGTGC GCCTCGAGGA GCAGCTGGCC TTCTCCGACT GGATTAACTC GAATCTGGGC 
CACGACAAGG ATCTGCAACA TCTGCTGCCC ATCGATAGCG AGGGCAAGCG CTTGTATCTG 
AGCATCAAGG ATGGTATTCT GCTGTGCAAA ATTATCAACC ACTCCTGCCC GGACACAATC 
GATGAGCGTG CGATCAACAA GAAGAACCTC ACCGTCTACC GGGAGTTTGA GAACTTGACC 
CTGGCCTTGG TTTCCTCCCA GGCGATTGGA TGCAACATCG TAAACATCGA TGCCCACGAT 
CTGGCCAAGG GCAAGCCACA TCTGGTGCTG GGTCTTCTCT GGCAGATCAT CCGCATCGGT 
CTGTTTAGCC ACATCACCCT GGACAGCTGT CCGGGATTGG CTGGCCTACT CTTCGACAAC 
GAACGTCTCG AGGATCTGAT GAAGATGTCG CCGGAGGCCA TCCTTTTGCG TTGGGTCAAC 
CATCATTTGG AGCGAGCCGG CATCTCGAGG CGGTGCACCA ACTTCCAGTC GGACATCGTC 
GATTCTGAGA TCTATTCGCA TTTGCTGAAG CAAATTGCCG GCAATGATGC TGATGTCAAT 
CTGGATGCCC TGCGGGAATC CGATCTGCAG TCGCGCGCGG AGATCATGTT GCAGCAGGCC 
GCCAAGCTCA ACTGCCGCAG CTTCCTCACG CCACAGGATG TCGTCAACGG AGTCTACAAA 
CTCAATCTAG CCTTCGTGGC TAATCTGTTC AACAACCATC CAGGATTGGA CAAGCCCGAG 
CAGATCGAGG GACTCGAGTC CATTGAGGAG ACGCGCGAAG AGAAGACCTA CCGCAATTGG 
ATGAACTCAA TGGGCGTGGC ACCGCACGTG AACTGGCTTT ACTCCGATTT GGCCGATGGT 
CTGGTCATTT TCCAGCTGTT CGACGTCATC AAGCCGGGTA TTGTCAACTG GAGCCGTGTG 
CACAAGCGTT TCAGCCCGCT GCGCAAGTTC ATGGAAAAGC TGGAGAACTG CAACTATGCG 
GTGGATCTGG GCAAGCAGCT CAAATTCTCG CTGGTCGGAA TCGCAGGCCA GGATCTAAAC 
GATGGCAATG CCACGCTGAC GTTGGCTCTC ATCTGGCAGC TTATGCGTGC CTACACCCTG 
TCCATTCTGT CCCGCTTGGC CAACACTGGC AACCCCATTA TCGAGAAGGA GATCGTCCAG 
TGGGTGAATA ACCGACTGTC GGAGGCAGGC AAACAGTCGC AGCTGCGTAA CTTCAACGAT 
CCGGCCATCG CCGATGGCAA GATCGTGATC GATCTGATCG ATGCCATCAA GGAGGGCAGC 
ATTAACTACG AGTTGGTGCG CACTAGCGGA ACACAGGAGG ATAACCTGGC CAATGCCAAG 
TATGCCATCT CCATGGCCCG CAAGATCGGC GCCCGTGTCT ACGCCCTGCC CGAGGACATC 
ACCGAGGTGA AGCCGAAAAT GGTGATGACC GTTTTCGCCT GCATGATGGC CCTCGACTAC 
GTGCCCAACA TGGACAGTGT GGACCAGAAC AACCACAACA GCTCCGCCAA CAACAGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523385.1
CDS	496..2418
Translation	MATLNKFTKTLSIDEKAEIKEKFIELDANKDGFIDLHELKDALNQVGFKLAGYQVREMIDEYKGKQLTAFQGKLNLEEFEALCLDLKSKDVASTFKTVVSKKENLETLGGMSSISSEGTTHSVRLEEQLAFSDWINSNLGHDKDLQHLLPIDSEGKRLYLSIKDGILLCKIINHSCPDTIDERAINKKNLTVYREFENLTLALVSSQAIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLAGLLFDNERLEDLMKMSPEAILLRWVNHHLERAGISRRCTNFQSDIVDSEIYSHLLKQIAGNDADVNLDALRESDLQSRAEIMLQQAAKLNCRSFLTPQDVVNGVYKLNLAFVANLFNNHPGLDKPEQIEGLESIEETREEKTYRNWMNSMGVAPHVNWLYSDLADGLVIFQLFDVIKPGIVNWSRVHKRFSPLRKFMEKLENCNYAVDLGKQLKFSLVGIAGQDLNDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIEKEIVQWVNNRLSEAGKQSQLRNFNDPAIADGKIVIDLIDAIKEGSINYELVRTSGTQEDNLANAKYAISMARKIGARVYALPEDITEVKPKMVMTVFACMMALDYVPNMDSVDQNNHNSSANNSN

Target ORF information:

RefSeq Version	NM_078661.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fimbrin, transcript variant A (Fim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078661.4

ORF Insert Sequence:

ATGGCAACAC TTAACAAATT CACAAAAACG CTGTCCATTG ATGAAAAGGC GGAGATCAAG 
GAGAAATTCA TAGAGTTGGA TGCCAACAAA GATGGCTTCA TCGATCTGCA CGAGCTAAAG 
GATGCCCTCA ACCAGGTGGG CTTCAAGCTG GCCGGCTACC AGGTGCGCGA AATGATTGAC 
GAGTACAAGG GCAAACAGCT GACTGCCTTC CAGGGCAAAT TGAATCTGGA GGAGTTCGAG 
GCACTGTGCC TGGACCTGAA GAGCAAGGAT GTGGCCAGCA CATTCAAGAC GGTCGTCTCC 
AAGAAGGAGA ACTTGGAGAC CCTGGGCGGC ATGTCGAGCA TTTCGTCGGA GGGCACCACC 
CATTCGGTGC GCCTCGAGGA GCAGCTGGCC TTCTCCGACT GGATTAACTC GAATCTGGGC 
CACGACAAGG ATCTGCAACA TCTGCTGCCC ATCGATAGCG AGGGCAAGCG CTTGTATCTG 
AGCATCAAGG ATGGTATTCT GCTGTGCAAA ATTATCAACC ACTCCTGCCC GGACACAATC 
GATGAGCGTG CGATCAACAA GAAGAACCTC ACCGTCTACC GGGAGTTTGA GAACTTGACC 
CTGGCCTTGG TTTCCTCCCA GGCGATTGGA TGCAACATCG TAAACATCGA TGCCCACGAT 
CTGGCCAAGG GCAAGCCACA TCTGGTGCTG GGTCTTCTCT GGCAGATCAT CCGCATCGGT 
CTGTTTAGCC ACATCACCCT GGACAGCTGT CCGGGATTGG CTGGCCTACT CTTCGACAAC 
GAACGTCTCG AGGATCTGAT GAAGATGTCG CCGGAGGCCA TCCTTTTGCG TTGGGTCAAC 
CATCATTTGG AGCGAGCCGG CATCTCGAGG CGGTGCACCA ACTTCCAGTC GGACATCGTC 
GATTCTGAGA TCTATTCGCA TTTGCTGAAG CAAATTGCCG GCAATGATGC TGATGTCAAT 
CTGGATGCCC TGCGGGAATC CGATCTGCAG TCGCGCGCGG AGATCATGTT GCAGCAGGCC 
GCCAAGCTCA ACTGCCGCAG CTTCCTCACG CCACAGGATG TCGTCAACGG AGTCTACAAA 
CTCAATCTAG CCTTCGTGGC TAATCTGTTC AACAACCATC CAGGATTGGA CAAGCCCGAG 
CAGATCGAGG GACTCGAGTC CATTGAGGAG ACGCGCGAAG AGAAGACCTA CCGCAATTGG 
ATGAACTCAA TGGGCGTGGC ACCGCACGTG AACTGGCTTT ACTCCGATTT GGCCGATGGT 
CTGGTCATTT TCCAGCTGTT CGACGTCATC AAGCCGGGTA TTGTCAACTG GAGCCGTGTG 
CACAAGCGTT TCAGCCCGCT GCGCAAGTTC ATGGAAAAGC TGGAGAACTG CAACTATGCG 
GTGGATCTGG GCAAGCAGCT CAAATTCTCG CTGGTCGGAA TCGCAGGCCA GGATCTAAAC 
GATGGCAATG CCACGCTGAC GTTGGCTCTC ATCTGGCAGC TTATGCGTGC CTACACCCTG 
TCCATTCTGT CCCGCTTGGC CAACACTGGC AACCCCATTA TCGAGAAGGA GATCGTCCAG 
TGGGTGAATA ACCGACTGTC GGAGGCAGGC AAACAGTCGC AGCTGCGTAA CTTCAACGAT 
CCGGCCATCG CCGATGGCAA GATCGTGATC GATCTGATCG ATGCCATCAA GGAGGGCAGC 
ATTAACTACG AGTTGGTGCG CACTAGCGGA ACACAGGAGG ATAACCTGGC CAATGCCAAG 
TATGCCATCT CCATGGCCCG CAAGATCGGC GCCCGTGTCT ACGCCCTGCC CGAGGACATC 
ACCGAGGTGA AGCCGAAAAT GGTGATGACC GTTTTCGCCT GCATGATGGC CCTCGACTAC 
GTGCCCAACA TGGACAGTGT GGACCAGAAC AACCACAACA GCTCCGCCAA CAACAGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fim ( NM_167564.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728073.1 Drosophila melanogaster fimbrin, transcript variant C (Fim), mRNA.

Price & Availability↑

CloneID	OFa01898
Clone ID Related Accession (Same CDS sequence)	NM_167564.2
Accession Version	NM_167564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fimbrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGATG CCAACAAAGA TGGCTTCATC GATCTGCACG AGCTAAAGGA TGCCCTCAAC 
CAGGTGGGCT TCAAGCTGGC CGGCTACCAG GTGCGCGAAA TGATTGACGA GTACAAGGGC 
AAACAGCTGA CTGCCTTCCA GGGCAAATTG AATCTGGAGG AGTTCGAGGC ACTGTGCCTG 
GACCTGAAGA GCAAGGATGT GGCCAGCACA TTCAAGACGG TCGTCTCCAA GAAGGAGAAC 
TTGGAGACCC TGGGCGGCAT GTCGAGCATT TCGTCGGAGG GCACCACCCA TTCGGTGCGC 
CTCGAGGAGC AGCTGGCCTT CTCCGACTGG ATTAACTCGA ATCTGGGCCA CGACAAGGAT 
CTGCAACATC TGCTGCCCAT CGATAGCGAG GGCAAGCGCT TGTATCTGAG CATCAAGGAT 
GGTATTCTGC TGTGCAAAAT TATCAACCAC TCCTGCCCGG ACACAATCGA TGAGCGTGCG 
ATCAACAAGA AGAACCTCAC CGTCTACCGG GAGTTTGAGA ACTTGACCCT GGCCTTGGTT 
TCCTCCCAGG CGATTGGATG CAACATCGTA AACATCGATG CCCACGATCT GGCCAAGGGC 
AAGCCACATC TGGTGCTGGG TCTTCTCTGG CAGATCATCC GCATCGGTCT GTTTAGCCAC 
ATCACCCTGG ACAGCTGTCC GGGATTGGCT GGCCTACTCT TCGACAACGA ACGTCTCGAG 
GATCTGATGA AGATGTCGCC GGAGGCCATC CTTTTGCGTT GGGTCAACCA TCATTTGGAG 
CGAGCCGGCA TCTCGAGGCG GTGCACCAAC TTCCAGTCGG ACATCGTCGA TTCTGAGATC 
TATTCGCATT TGCTGAAGCA AATTGCCGGC AATGATGCTG ATGTCAATCT GGATGCCCTG 
CGGGAATCCG ATCTGCAGTC GCGCGCGGAG ATCATGTTGC AGCAGGCCGC CAAGCTCAAC 
TGCCGCAGCT TCCTCACGCC ACAGGATGTC GTCAACGGAG TCTACAAACT CAATCTAGCC 
TTCGTGGCTA ATCTGTTCAA CAACCATCCA GGATTGGACA AGCCCGAGCA GATCGAGGGA 
CTCGAGTCCA TTGAGGAGAC GCGCGAAGAG AAGACCTACC GCAATTGGAT GAACTCAATG 
GGCGTGGCAC CGCACGTGAA CTGGCTTTAC TCCGATTTGG CCGATGGTCT GGTCATTTTC 
CAGCTGTTCG ACGTCATCAA GCCGGGTATT GTCAACTGGA GCCGTGTGCA CAAGCGTTTC 
AGCCCGCTGC GCAAGTTCAT GGAAAAGCTG GAGAACTGCA ACTATGCGGT GGATCTGGGC 
AAGCAGCTCA AATTCTCGCT GGTCGGAATC GCAGGCCAGG ATCTAAACGA TGGCAATGCC 
ACGCTGACGT TGGCTCTCAT CTGGCAGCTT ATGCGTGCCT ACACCCTGTC CATTCTGTCC 
CGCTTGGCCA ACACTGGCAA CCCCATTATC GAGAAGGAGA TCGTCCAGTG GGTGAATAAC 
CGACTGTCGG AGGCAGGCAA ACAGTCGCAG CTGCGTAACT TCAACGATCC GGCCATCGCC 
GATGGCAAGA TCGTGATCGA TCTGATCGAT GCCATCAAGG AGGGCAGCAT TAACTACGAG 
TTGGTGCGCA CTAGCGGAAC ACAGGAGGAT AACCTGGCCA ATGCCAAGTA TGCCATCTCC 
ATGGCCCGCA AGATCGGCGC CCGTGTCTAC GCCCTGCCCG AGGACATCAC CGAGGTGAAG 
CCGAAAATGG TGATGACCGT TTTCGCCTGC ATGATGGCCC TCGACTACGT GCCCAACATG 
GACAGTGTGG ACCAGAACAA CCACAACAGC TCCGCCAACA ACAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728073.1
CDS	450..2300
Translation	MLDANKDGFIDLHELKDALNQVGFKLAGYQVREMIDEYKGKQLTAFQGKLNLEEFEALCLDLKSKDVASTFKTVVSKKENLETLGGMSSISSEGTTHSVRLEEQLAFSDWINSNLGHDKDLQHLLPIDSEGKRLYLSIKDGILLCKIINHSCPDTIDERAINKKNLTVYREFENLTLALVSSQAIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLAGLLFDNERLEDLMKMSPEAILLRWVNHHLERAGISRRCTNFQSDIVDSEIYSHLLKQIAGNDADVNLDALRESDLQSRAEIMLQQAAKLNCRSFLTPQDVVNGVYKLNLAFVANLFNNHPGLDKPEQIEGLESIEETREEKTYRNWMNSMGVAPHVNWLYSDLADGLVIFQLFDVIKPGIVNWSRVHKRFSPLRKFMEKLENCNYAVDLGKQLKFSLVGIAGQDLNDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIEKEIVQWVNNRLSEAGKQSQLRNFNDPAIADGKIVIDLIDAIKEGSINYELVRTSGTQEDNLANAKYAISMARKIGARVYALPEDITEVKPKMVMTVFACMMALDYVPNMDSVDQNNHNSSANNSN

Target ORF information:

RefSeq Version	NM_167564.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fimbrin, transcript variant C (Fim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167564.2

ORF Insert Sequence:

ATGTTGGATG CCAACAAAGA TGGCTTCATC GATCTGCACG AGCTAAAGGA TGCCCTCAAC 
CAGGTGGGCT TCAAGCTGGC CGGCTACCAG GTGCGCGAAA TGATTGACGA GTACAAGGGC 
AAACAGCTGA CTGCCTTCCA GGGCAAATTG AATCTGGAGG AGTTCGAGGC ACTGTGCCTG 
GACCTGAAGA GCAAGGATGT GGCCAGCACA TTCAAGACGG TCGTCTCCAA GAAGGAGAAC 
TTGGAGACCC TGGGCGGCAT GTCGAGCATT TCGTCGGAGG GCACCACCCA TTCGGTGCGC 
CTCGAGGAGC AGCTGGCCTT CTCCGACTGG ATTAACTCGA ATCTGGGCCA CGACAAGGAT 
CTGCAACATC TGCTGCCCAT CGATAGCGAG GGCAAGCGCT TGTATCTGAG CATCAAGGAT 
GGTATTCTGC TGTGCAAAAT TATCAACCAC TCCTGCCCGG ACACAATCGA TGAGCGTGCG 
ATCAACAAGA AGAACCTCAC CGTCTACCGG GAGTTTGAGA ACTTGACCCT GGCCTTGGTT 
TCCTCCCAGG CGATTGGATG CAACATCGTA AACATCGATG CCCACGATCT GGCCAAGGGC 
AAGCCACATC TGGTGCTGGG TCTTCTCTGG CAGATCATCC GCATCGGTCT GTTTAGCCAC 
ATCACCCTGG ACAGCTGTCC GGGATTGGCT GGCCTACTCT TCGACAACGA ACGTCTCGAG 
GATCTGATGA AGATGTCGCC GGAGGCCATC CTTTTGCGTT GGGTCAACCA TCATTTGGAG 
CGAGCCGGCA TCTCGAGGCG GTGCACCAAC TTCCAGTCGG ACATCGTCGA TTCTGAGATC 
TATTCGCATT TGCTGAAGCA AATTGCCGGC AATGATGCTG ATGTCAATCT GGATGCCCTG 
CGGGAATCCG ATCTGCAGTC GCGCGCGGAG ATCATGTTGC AGCAGGCCGC CAAGCTCAAC 
TGCCGCAGCT TCCTCACGCC ACAGGATGTC GTCAACGGAG TCTACAAACT CAATCTAGCC 
TTCGTGGCTA ATCTGTTCAA CAACCATCCA GGATTGGACA AGCCCGAGCA GATCGAGGGA 
CTCGAGTCCA TTGAGGAGAC GCGCGAAGAG AAGACCTACC GCAATTGGAT GAACTCAATG 
GGCGTGGCAC CGCACGTGAA CTGGCTTTAC TCCGATTTGG CCGATGGTCT GGTCATTTTC 
CAGCTGTTCG ACGTCATCAA GCCGGGTATT GTCAACTGGA GCCGTGTGCA CAAGCGTTTC 
AGCCCGCTGC GCAAGTTCAT GGAAAAGCTG GAGAACTGCA ACTATGCGGT GGATCTGGGC 
AAGCAGCTCA AATTCTCGCT GGTCGGAATC GCAGGCCAGG ATCTAAACGA TGGCAATGCC 
ACGCTGACGT TGGCTCTCAT CTGGCAGCTT ATGCGTGCCT ACACCCTGTC CATTCTGTCC 
CGCTTGGCCA ACACTGGCAA CCCCATTATC GAGAAGGAGA TCGTCCAGTG GGTGAATAAC 
CGACTGTCGG AGGCAGGCAA ACAGTCGCAG CTGCGTAACT TCAACGATCC GGCCATCGCC 
GATGGCAAGA TCGTGATCGA TCTGATCGAT GCCATCAAGG AGGGCAGCAT TAACTACGAG 
TTGGTGCGCA CTAGCGGAAC ACAGGAGGAT AACCTGGCCA ATGCCAAGTA TGCCATCTCC 
ATGGCCCGCA AGATCGGCGC CCGTGTCTAC GCCCTGCCCG AGGACATCAC CGAGGTGAAG 
CCGAAAATGG TGATGACCGT TTTCGCCTGC ATGATGGCCC TCGACTACGT GCCCAACATG 
GACAGTGTGG ACCAGAACAA CCACAACAGC TCCGCCAACA ACAGCAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fim ( NM_167565.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728074.1 Drosophila melanogaster fimbrin, transcript variant D (Fim), mRNA.

Price & Availability↑

CloneID	OFa01899
Clone ID Related Accession (Same CDS sequence)	NM_167565.2
Accession Version	NM_167565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fimbrin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167565.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATA TTCTCAACGT GGAGCAGCAC CAAACGGTTC AGGAGTTCAA GCAGAATCTC 
GAGGAGCTCA TTCCCAACTT GGATGCCAAC AAAGATGGCT TCATCGATCT GCACGAGCTA 
AAGGATGCCC TCAACCAGGT GGGCTTCAAG CTGGCCGGCT ACCAGGTGCG CGAAATGATT 
GACGAGTACA AGGGCAAACA GCTGACTGCC TTCCAGGGCA AATTGAATCT GGAGGAGTTC 
GAGGCACTGT GCCTGGACCT GAAGAGCAAG GATGTGGCCA GCACATTCAA GACGGTCGTC 
TCCAAGAAGG AGAACTTGGA GACCCTGGGC GGCATGTCGA GCATTTCGTC GGAGGGCACC 
ACCCATTCGG TGCGCCTCGA GGAGCAGCTG GCCTTCTCCG ACTGGATTAA CTCGAATCTG 
GGCCACGACA AGGATCTGCA ACATCTGCTG CCCATCGATA GCGAGGGCAA GCGCTTGTAT 
CTGAGCATCA AGGATGGTAT TCTGCTGTGC AAAATTATCA ACCACTCCTG CCCGGACACA 
ATCGATGAGC GTGCGATCAA CAAGAAGAAC CTCACCGTCT ACCGGGAGTT TGAGAACTTG 
ACCCTGGCCT TGGTTTCCTC CCAGGCGATT GGATGCAACA TCGTAAACAT CGATGCCCAC 
GATCTGGCCA AGGGCAAGCC ACATCTGGTG CTGGGTCTTC TCTGGCAGAT CATCCGCATC 
GGTCTGTTTA GCCACATCAC CCTGGACAGC TGTCCGGGAT TGGCTGGCCT ACTCTTCGAC 
AACGAACGTC TCGAGGATCT GATGAAGATG TCGCCGGAGG CCATCCTTTT GCGTTGGGTC 
AACCATCATT TGGAGCGAGC CGGCATCTCG AGGCGGTGCA CCAACTTCCA GTCGGACATC 
GTCGATTCTG AGATCTATTC GCATTTGCTG AAGCAAATTG CCGGCAATGA TGCTGATGTC 
AATCTGGATG CCCTGCGGGA ATCCGATCTG CAGTCGCGCG CGGAGATCAT GTTGCAGCAG 
GCCGCCAAGC TCAACTGCCG CAGCTTCCTC ACGCCACAGG ATGTCGTCAA CGGAGTCTAC 
AAACTCAATC TAGCCTTCGT GGCTAATCTG TTCAACAACC ATCCAGGATT GGACAAGCCC 
GAGCAGATCG AGGGACTCGA GTCCATTGAG GAGACGCGCG AAGAGAAGAC CTACCGCAAT 
TGGATGAACT CAATGGGCGT GGCACCGCAC GTGAACTGGC TTTACTCCGA TTTGGCCGAT 
GGTCTGGTCA TTTTCCAGCT GTTCGACGTC ATCAAGCCGG GTATTGTCAA CTGGAGCCGT 
GTGCACAAGC GTTTCAGCCC GCTGCGCAAG TTCATGGAAA AGCTGGAGAA CTGCAACTAT 
GCGGTGGATC TGGGCAAGCA GCTCAAATTC TCGCTGGTCG GAATCGCAGG CCAGGATCTA 
AACGATGGCA ATGCCACGCT GACGTTGGCT CTCATCTGGC AGCTTATGCG TGCCTACACC 
CTGTCCATTC TGTCCCGCTT GGCCAACACT GGCAACCCCA TTATCGAGAA GGAGATCGTC 
CAGTGGGTGA ATAACCGACT GTCGGAGGCA GGCAAACAGT CGCAGCTGCG TAACTTCAAC 
GATCCGGCCA TCGCCGATGG CAAGATCGTG ATCGATCTGA TCGATGCCAT CAAGGAGGGC 
AGCATTAACT ACGAGTTGGT GCGCACTAGC GGAACACAGG AGGATAACCT GGCCAATGCC 
AAGTATGCCA TCTCCATGGC CCGCAAGATC GGCGCCCGTG TCTACGCCCT GCCCGAGGAC 
ATCACCGAGG TGAAGCCGAA AATGGTGATG ACCGTTTTCG CCTGCATGAT GGCCCTCGAC 
TACGTGCCCA ACATGGACAG TGTGGACCAG AACAACCACA ACAGCTCCGC CAACAACAGC 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728074.1
CDS	342..2267
Translation	MADILNVEQHQTVQEFKQNLEELIPNLDANKDGFIDLHELKDALNQVGFKLAGYQVREMIDEYKGKQLTAFQGKLNLEEFEALCLDLKSKDVASTFKTVVSKKENLETLGGMSSISSEGTTHSVRLEEQLAFSDWINSNLGHDKDLQHLLPIDSEGKRLYLSIKDGILLCKIINHSCPDTIDERAINKKNLTVYREFENLTLALVSSQAIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLAGLLFDNERLEDLMKMSPEAILLRWVNHHLERAGISRRCTNFQSDIVDSEIYSHLLKQIAGNDADVNLDALRESDLQSRAEIMLQQAAKLNCRSFLTPQDVVNGVYKLNLAFVANLFNNHPGLDKPEQIEGLESIEETREEKTYRNWMNSMGVAPHVNWLYSDLADGLVIFQLFDVIKPGIVNWSRVHKRFSPLRKFMEKLENCNYAVDLGKQLKFSLVGIAGQDLNDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIEKEIVQWVNNRLSEAGKQSQLRNFNDPAIADGKIVIDLIDAIKEGSINYELVRTSGTQEDNLANAKYAISMARKIGARVYALPEDITEVKPKMVMTVFACMMALDYVPNMDSVDQNNHNSSANNSN

Target ORF information:

RefSeq Version	NM_167565.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fimbrin, transcript variant D (Fim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167565.2

ORF Insert Sequence:

ATGGCCGATA TTCTCAACGT GGAGCAGCAC CAAACGGTTC AGGAGTTCAA GCAGAATCTC 
GAGGAGCTCA TTCCCAACTT GGATGCCAAC AAAGATGGCT TCATCGATCT GCACGAGCTA 
AAGGATGCCC TCAACCAGGT GGGCTTCAAG CTGGCCGGCT ACCAGGTGCG CGAAATGATT 
GACGAGTACA AGGGCAAACA GCTGACTGCC TTCCAGGGCA AATTGAATCT GGAGGAGTTC 
GAGGCACTGT GCCTGGACCT GAAGAGCAAG GATGTGGCCA GCACATTCAA GACGGTCGTC 
TCCAAGAAGG AGAACTTGGA GACCCTGGGC GGCATGTCGA GCATTTCGTC GGAGGGCACC 
ACCCATTCGG TGCGCCTCGA GGAGCAGCTG GCCTTCTCCG ACTGGATTAA CTCGAATCTG 
GGCCACGACA AGGATCTGCA ACATCTGCTG CCCATCGATA GCGAGGGCAA GCGCTTGTAT 
CTGAGCATCA AGGATGGTAT TCTGCTGTGC AAAATTATCA ACCACTCCTG CCCGGACACA 
ATCGATGAGC GTGCGATCAA CAAGAAGAAC CTCACCGTCT ACCGGGAGTT TGAGAACTTG 
ACCCTGGCCT TGGTTTCCTC CCAGGCGATT GGATGCAACA TCGTAAACAT CGATGCCCAC 
GATCTGGCCA AGGGCAAGCC ACATCTGGTG CTGGGTCTTC TCTGGCAGAT CATCCGCATC 
GGTCTGTTTA GCCACATCAC CCTGGACAGC TGTCCGGGAT TGGCTGGCCT ACTCTTCGAC 
AACGAACGTC TCGAGGATCT GATGAAGATG TCGCCGGAGG CCATCCTTTT GCGTTGGGTC 
AACCATCATT TGGAGCGAGC CGGCATCTCG AGGCGGTGCA CCAACTTCCA GTCGGACATC 
GTCGATTCTG AGATCTATTC GCATTTGCTG AAGCAAATTG CCGGCAATGA TGCTGATGTC 
AATCTGGATG CCCTGCGGGA ATCCGATCTG CAGTCGCGCG CGGAGATCAT GTTGCAGCAG 
GCCGCCAAGC TCAACTGCCG CAGCTTCCTC ACGCCACAGG ATGTCGTCAA CGGAGTCTAC 
AAACTCAATC TAGCCTTCGT GGCTAATCTG TTCAACAACC ATCCAGGATT GGACAAGCCC 
GAGCAGATCG AGGGACTCGA GTCCATTGAG GAGACGCGCG AAGAGAAGAC CTACCGCAAT 
TGGATGAACT CAATGGGCGT GGCACCGCAC GTGAACTGGC TTTACTCCGA TTTGGCCGAT 
GGTCTGGTCA TTTTCCAGCT GTTCGACGTC ATCAAGCCGG GTATTGTCAA CTGGAGCCGT 
GTGCACAAGC GTTTCAGCCC GCTGCGCAAG TTCATGGAAA AGCTGGAGAA CTGCAACTAT 
GCGGTGGATC TGGGCAAGCA GCTCAAATTC TCGCTGGTCG GAATCGCAGG CCAGGATCTA 
AACGATGGCA ATGCCACGCT GACGTTGGCT CTCATCTGGC AGCTTATGCG TGCCTACACC 
CTGTCCATTC TGTCCCGCTT GGCCAACACT GGCAACCCCA TTATCGAGAA GGAGATCGTC 
CAGTGGGTGA ATAACCGACT GTCGGAGGCA GGCAAACAGT CGCAGCTGCG TAACTTCAAC 
GATCCGGCCA TCGCCGATGG CAAGATCGTG ATCGATCTGA TCGATGCCAT CAAGGAGGGC 
AGCATTAACT ACGAGTTGGT GCGCACTAGC GGAACACAGG AGGATAACCT GGCCAATGCC 
AAGTATGCCA TCTCCATGGC CCGCAAGATC GGCGCCCGTG TCTACGCCCT GCCCGAGGAC 
ATCACCGAGG TGAAGCCGAA AATGGTGATG ACCGTTTTCG CCTGCATGAT GGCCCTCGAC 
TACGTGCCCA ACATGGACAG TGTGGACCAG AACAACCACA ACAGCTCCGC CAACAACAGC 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fim cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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