CDC50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC50
Entrez Gene ID	32615
Full Name	CG9947 gene product from transcript CG9947-RB
Synonyms	Dmel\CG9947
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14C4-14C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298398.2	NP_001285327.1	CDC50, isoform B
NM_132900.3	NP_573128.2	CDC50, isoform A

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G54320	NP_564656.1
Oryza sativa (rice)	Os06g0665000	NP_001058302.1
Canis lupus familiaris (dog)	TMEM30A	XP_867464.3
Saccharomyces cerevisiae (baker's yeast)	YNR048W	NP_014446.3
Schizosaccharomyces pombe (fission yeast)	mug89	NP_595126.1
Magnaporthe oryzae (rice blast fungus)	MGG_12405	XP_003715571.1
	TMEM30A	XP_001109918.2
Caenorhabditis elegans (roundworm)	chat-1	NP_001023332.1
Arabidopsis thaliana (thale cress)	AT1G16360	NP_173086.2
Xenopus tropicalis (tropical clawed frog)	tmem30a	NP_989133.1
Bos taurus (cattle)	TMEM30A	NP_001068691.1
Mus musculus (house mouse)	Tmem30a	NP_598479.1
Gallus gallus (chicken)	TMEM30A	NP_001012897.1
Homo sapiens (human)	TMEM30A	NP_060717.1
Pan troglodytes (chimpanzee)	TMEM30A	XP_001143816.1
Rattus norvegicus (Norway rat)	Tmem30a	NP_001004248.1
Drosophila melanogaster (fruit fly)	CG9947	NP_573128.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000704	XP_311234.5
Neurospora crassa	NCU01165	XP_961531.1
Arabidopsis thaliana (thale cress)	ALIS5	NP_565210.1
Arabidopsis thaliana (thale cress)	ALIS1	NP_566435.1
Oryza sativa (rice)	Os02g0173800	NP_001046043.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDC50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01802	NM_132900.3 Latest version!	Drosophila melanogaster CDC50, transcript variant A (CDC50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21501	NM_001298398.2 Latest version!	Drosophila melanogaster CDC50, transcript variant B (CDC50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC50 ( NM_132900.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573128.2 Drosophila melanogaster CDC50, transcript variant A (CDC50), mRNA.

Price & Availability↑

CloneID	OFa01802
Clone ID Related Accession (Same CDS sequence)	NM_132900.3
Accession Version	NM_132900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CDC50, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132900.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACAC AGCAAGCGGA GAATGAGGAG AGTGCAGCGG CGAAGTCCAA GCGACCTTCA 
GATTCGGCCT TCAAGCAACA GCGCCTGCCC GCCTGGCAGC CCGTCCTGAC CGCCCGCACC 
GTACTGCCCA CCTTCTTCGT TATCGGAGTT CTATTCATAC CCATCGGCGT GGTCCTGCTC 
CACCTGAGCA ACACCGCCAA CGAGCTGATC ATCGACTACA CCAAGTGCAG GCGCTCGGGC 
GGAAATACCA CCTGTGCGGA GTATCTGGAG GCCAACCCCG GCGTTACGTG CCCCTGCGAG 
GTGCCCTTCG TCCTGCCCTC GGACTTCAAT GGTGTGGTAT ACATGTACTA CGGACTGACC 
AACTACTATC AGAACCATCG GCGCTATGTG AAGTCACGGG ACGACGAGCA GCTGCTGGGT 
CACCTGTCGC AAACGCCCAG TACAGACTGT GCCCCCTTCG CCTATGATCC CGATTCCGGA 
AAGCCCATCG CGCCCTGCGG TGCCATTGCC AACTCGCTGT TCAATGACAC GCTGACCCTC 
TTGCAGGGTG GATCCGAGAT CAAGCTGCTG AAGACGGGCA TTGCCTGGCC GTCGGACAAG 
CGCGTCAAGT TCCGAAACCC AGAGGGTAAC CTGAACGTCT CCCTTGAGGG CTTCTCAAAG 
CCAATTTTCT GGCAGAAGGG TCTGGCCGAC CTGGACCCGG AGAATCCGGA CAACAATGGC 
TTCCAGAACG AGGATCTGAT CGTGTGGATG CGCACCGCCG CTCTGCCCAG CTTCCGGAAG 
CTATATCGCC GTCTCAACCA AACGAACACC AACTATGCCA ACGGCCTGAA GTCTGGCAAT 
TATACGCTTA ACATTAAGTA TAATTATCCG GTGGTGTCGT TCGACGGCAC GAAGCGAATG 
ATACTCTCCA CCACATCCGT GCTCGGTGGC AAGAACCCGT TCCTGGGCAT TGCGTACATT 
GTGGTGGGTG CCATTTGCAT CACCCTGGGC CTGGCCCTGC TATTTATTCA TATGCGATGC 
AGTCGCAGCA ACATGGAGAT GATCAACGTG AACCCGCACA CGCAGTACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573128.2
CDS	95..1168
Translation	MSTQQAENEESAAAKSKRPSDSAFKQQRLPAWQPVLTARTVLPTFFVIGVLFIPIGVVLLHLSNTANELIIDYTKCRRSGGNTTCAEYLEANPGVTCPCEVPFVLPSDFNGVVYMYYGLTNYYQNHRRYVKSRDDEQLLGHLSQTPSTDCAPFAYDPDSGKPIAPCGAIANSLFNDTLTLLQGGSEIKLLKTGIAWPSDKRVKFRNPEGNLNVSLEGFSKPIFWQKGLADLDPENPDNNGFQNEDLIVWMRTAALPSFRKLYRRLNQTNTNYANGLKSGNYTLNIKYNYPVVSFDGTKRMILSTTSVLGGKNPFLGIAYIVVGAICITLGLALLFIHMRCSRSNMEMINVNPHTQYS

Target ORF information:

RefSeq Version	NM_132900.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CDC50, transcript variant A (CDC50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132900.3

ORF Insert Sequence:

ATGTCCACAC AGCAAGCGGA GAATGAGGAG AGTGCAGCGG CGAAGTCCAA GCGACCTTCA 
GATTCGGCCT TCAAGCAACA GCGCCTGCCC GCCTGGCAGC CCGTCCTGAC CGCCCGCACC 
GTACTGCCCA CCTTCTTCGT TATCGGAGTT CTATTCATAC CCATCGGCGT GGTCCTGCTC 
CACCTGAGCA ACACCGCCAA CGAGCTGATC ATCGACTACA CCAAGTGCAG GCGCTCGGGC 
GGAAATACCA CCTGTGCGGA GTATCTGGAG GCCAACCCCG GCGTTACGTG CCCCTGCGAG 
GTGCCCTTCG TCCTGCCCTC GGACTTCAAT GGTGTGGTAT ACATGTACTA CGGACTGACC 
AACTACTATC AGAACCATCG GCGCTATGTG AAGTCACGGG ACGACGAGCA GCTGCTGGGT 
CACCTGTCGC AAACGCCCAG TACAGACTGT GCCCCCTTCG CCTATGATCC CGATTCCGGA 
AAGCCCATCG CGCCCTGCGG TGCCATTGCC AACTCGCTGT TCAATGACAC GCTGACCCTC 
TTGCAGGGTG GATCCGAGAT CAAGCTGCTG AAGACGGGCA TTGCCTGGCC GTCGGACAAG 
CGCGTCAAGT TCCGAAACCC AGAGGGTAAC CTGAACGTCT CCCTTGAGGG CTTCTCAAAG 
CCAATTTTCT GGCAGAAGGG TCTGGCCGAC CTGGACCCGG AGAATCCGGA CAACAATGGC 
TTCCAGAACG AGGATCTGAT CGTGTGGATG CGCACCGCCG CTCTGCCCAG CTTCCGGAAG 
CTATATCGCC GTCTCAACCA AACGAACACC AACTATGCCA ACGGCCTGAA GTCTGGCAAT 
TATACGCTTA ACATTAAGTA TAATTATCCG GTGGTGTCGT TCGACGGCAC GAAGCGAATG 
ATACTCTCCA CCACATCCGT GCTCGGTGGC AAGAACCCGT TCCTGGGCAT TGCGTACATT 
GTGGTGGGTG CCATTTGCAT CACCCTGGGC CTGGCCCTGC TATTTATTCA TATGCGATGC 
AGTCGCAGCA ACATGGAGAT GATCAACGTG AACCCGCACA CGCAGTACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC50 ( NM_001298398.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285327.1 Drosophila melanogaster CDC50, transcript variant B (CDC50), mRNA.

Price & Availability↑

CloneID	OFa21501
Clone ID Related Accession (Same CDS sequence)	NM_001298398.2
Accession Version	NM_001298398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CDC50, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001298398.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACAC AGCAAGCGGA GAATGAGGAG AGTGCAGCGG CGAAGTCCAA GCGACCTTCA 
GATTCGGCCT TCAAGCAACA GCGCCTGCCC GCCTGGCAGC CCGTCCTGAC CGCCCGCACC 
GTACTGCCCA CCTTCTTCGT TATCGGAGTT CTATTCATAC CCATCGGCGT GGTCCTGCTC 
CACCTGAGCA ACACCGCCAA CGAGCTGATC ATCGACTACA CCAAGTGCAG GCGCTCGGGC 
GGAAATACCA CCTGTGCGGA GTATCTGGAG GCCAACCCCG GCGTTACGTG CCCCTGCGAG 
GTGCCCTTCG TCCTGCCCTC GGACTTCAAT GGTGTGGTAT ACATGTACTA CGGACTGACC 
AACTACTATC AGAACCATCG GCGCTATGTG AAGTCACGGG ACGACGAGCA GCTGCTGGGT 
CACCTGTCGC AAACGCCCAG TACAGACTGT GCCCCCTTCG CCTATGATCC CGATTCCGGA 
AAGCCCATCG CGCCCTGCGG TGCCATTGCC AACTCGCTGT TCAATGACAC GCTGACCCTC 
TTGCAGGGTG GATCCGAGAT CAAGCTGCTG AAGACGGGCA TTGCCTGGCC GTCGGACAAG 
CGCGTCAAGT TCCGAAACCC AGAGGGTAAC CTGAACGTCT CCCTTGAGGG CTTCTCAAAG 
CCAATTTTCT GGCAGAAGGG TCTGGCCGAC CTGGACCCGG AGAATCCGGA CAACAATGGC 
TTCCAGAACG AGGATCTGAT CGTGTGGATG CGCACCGCCG CTCTGCCCAG CTTCCGGAAG 
CTATATCGCC GTCTCAACCA AACGAACACC AACTATGCCA ACGGCCTGAA GTCTGGCAAT 
TATACGCTTA ACATTAAGTA TAATTATCCG GTGGTGTCGT TCGACGGCAC GAAGCGAATG 
ATACTCTCCA CCACATCCGT GCTCGGTGGC AAGAACCCGT TCCTGGGCAT TGCGTACATT 
GTGGTGGGTG CCATTTGCAT CACCCTGGGC CTGGCCCTGC TATTTATTCA TATGCGATGC 
AGTCGCAGCA TTATAGTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285327.1
CDS	95..1138
Translation	MSTQQAENEESAAAKSKRPSDSAFKQQRLPAWQPVLTARTVLPTFFVIGVLFIPIGVVLLHLSNTANELIIDYTKCRRSGGNTTCAEYLEANPGVTCPCEVPFVLPSDFNGVVYMYYGLTNYYQNHRRYVKSRDDEQLLGHLSQTPSTDCAPFAYDPDSGKPIAPCGAIANSLFNDTLTLLQGGSEIKLLKTGIAWPSDKRVKFRNPEGNLNVSLEGFSKPIFWQKGLADLDPENPDNNGFQNEDLIVWMRTAALPSFRKLYRRLNQTNTNYANGLKSGNYTLNIKYNYPVVSFDGTKRMILSTTSVLGGKNPFLGIAYIVVGAICITLGLALLFIHMRCSRSIIVH

Target ORF information:

RefSeq Version	NM_001298398.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CDC50, transcript variant B (CDC50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298398.2

ORF Insert Sequence:

ATGTCCACAC AGCAAGCGGA GAATGAGGAG AGTGCAGCGG CGAAGTCCAA GCGACCTTCA 
GATTCGGCCT TCAAGCAACA GCGCCTGCCC GCCTGGCAGC CCGTCCTGAC CGCCCGCACC 
GTACTGCCCA CCTTCTTCGT TATCGGAGTT CTATTCATAC CCATCGGCGT GGTCCTGCTC 
CACCTGAGCA ACACCGCCAA CGAGCTGATC ATCGACTACA CCAAGTGCAG GCGCTCGGGC 
GGAAATACCA CCTGTGCGGA GTATCTGGAG GCCAACCCCG GCGTTACGTG CCCCTGCGAG 
GTGCCCTTCG TCCTGCCCTC GGACTTCAAT GGTGTGGTAT ACATGTACTA CGGACTGACC 
AACTACTATC AGAACCATCG GCGCTATGTG AAGTCACGGG ACGACGAGCA GCTGCTGGGT 
CACCTGTCGC AAACGCCCAG TACAGACTGT GCCCCCTTCG CCTATGATCC CGATTCCGGA 
AAGCCCATCG CGCCCTGCGG TGCCATTGCC AACTCGCTGT TCAATGACAC GCTGACCCTC 
TTGCAGGGTG GATCCGAGAT CAAGCTGCTG AAGACGGGCA TTGCCTGGCC GTCGGACAAG 
CGCGTCAAGT TCCGAAACCC AGAGGGTAAC CTGAACGTCT CCCTTGAGGG CTTCTCAAAG 
CCAATTTTCT GGCAGAAGGG TCTGGCCGAC CTGGACCCGG AGAATCCGGA CAACAATGGC 
TTCCAGAACG AGGATCTGAT CGTGTGGATG CGCACCGCCG CTCTGCCCAG CTTCCGGAAG 
CTATATCGCC GTCTCAACCA AACGAACACC AACTATGCCA ACGGCCTGAA GTCTGGCAAT 
TATACGCTTA ACATTAAGTA TAATTATCCG GTGGTGTCGT TCGACGGCAC GAAGCGAATG 
ATACTCTCCA CCACATCCGT GCTCGGTGGC AAGAACCCGT TCCTGGGCAT TGCGTACATT 
GTGGTGGGTG CCATTTGCAT CACCCTGGGC CTGGCCCTGC TATTTATTCA TATGCGATGC 
AGTCGCAGCA TTATAGTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CDC50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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