TMEM30A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM30A
Entrez Gene ID	55754
Full Name	transmembrane protein 30A
Synonyms	C6orf67,CDC50A
General protein information	Preferred Names transmembrane protein 30A Names cell cycle control protein 50A P4-ATPase flippase complex beta subunit TMEM30A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q14.1
Disorder MIM:	611028

mRNA and Protein(s)

mRNA	Protein	Name
NM_018247.4	NP_060717.1	cell cycle control protein 50A isoform 1
NM_018247.3	NP_060717.1	cell cycle control protein 50A isoform 1
NM_001143958.1	NP_001137430.1	cell cycle control protein 50A isoform 2
NM_001143958.2	NP_001137430.1	cell cycle control protein 50A isoform 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G54320	NP_564656.1
Oryza sativa (rice)	Os06g0665000	NP_001058302.1
Canis lupus familiaris (dog)	TMEM30A	XP_867464.3
Saccharomyces cerevisiae (baker's yeast)	YNR048W	NP_014446.3
Schizosaccharomyces pombe (fission yeast)	mug89	NP_595126.1
Magnaporthe oryzae (rice blast fungus)	MGG_12405	XP_003715571.1
	TMEM30A	XP_001109918.2
Caenorhabditis elegans (roundworm)	chat-1	NP_001023332.1
Arabidopsis thaliana (thale cress)	AT1G16360	NP_173086.2
Xenopus tropicalis (tropical clawed frog)	tmem30a	NP_989133.1
Bos taurus (cattle)	TMEM30A	NP_001068691.1
Mus musculus (house mouse)	Tmem30a	NP_598479.1
Gallus gallus (chicken)	TMEM30A	NP_001012897.1
Homo sapiens (human)	TMEM30A	NP_060717.1
Pan troglodytes (chimpanzee)	TMEM30A	XP_001143816.1
Rattus norvegicus (Norway rat)	Tmem30a	NP_001004248.1
Drosophila melanogaster (fruit fly)	CG9947	NP_573128.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000704	XP_311234.5
Neurospora crassa	NCU01165	XP_961531.1
Arabidopsis thaliana (thale cress)	ALIS5	NP_565210.1
Arabidopsis thaliana (thale cress)	ALIS1	NP_566435.1
Oryza sativa (rice)	Os02g0173800	NP_001046043.1

Gene Ontology
Bibliography

Related articles in PubMed

The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane.
Segawa K, Kurata S, Nagata S
The Journal of biological chemistry(2017 Dec)

The lipid flippase heterodimer ATP8B1-CDC50A is essential for surface expression of the apical sodium-dependent bile acid transporter (SLC10A2/ASBT) in intestinal Caco-2 cells.
van der Mark VA, de Waart DR, Ho-Mok KS, Tabbers MM, Voogt HW, Oude Elferink RP, Knisely AS, Paulusma CC
Biochimica et biophysica acta1842(12 Pt A)2378-86(2014 Dec)

Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure.
Segawa K, Kurata S, Yanagihashi Y, Brummelkamp TR, Matsuda F, Nagata S
Science (New York, N.Y.)344(6188)1164-8(2014 Jun)

Role for phospholipid flippase complex of ATP8A1 and CDC50A proteins in cell migration.
Kato U, Inadome H, Yamamoto M, Emoto K, Kobayashi T, Umeda M
The Journal of biological chemistry288(7)4922-34(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the lipid flippase (ATP8B1)-transmembrane protein 30A (CDC50A) heterodimer is essential for the apical localization of sodium-dependent bile acid transporter (SLC10A2/ASBT) in Caco-2 cells.
Title: The lipid flippase heterodimer ATP8B1-CDC50A is essential for surface expression of the apical sodium-dependent bile acid transporter (SLC10A2/ASBT) in intestinal Caco-2 cells.

ATP11C and CDC50A are required for aminophospholipid translocation from the outer to the inner plasma membrane leaflet; that is, they display flippase activity.
Title: Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure.

the phospholipid flippase complex of ATP8A1 and CDC50A proteins has a role in cell migration
Title: Role for phospholipid flippase complex of ATP8A1 and CDC50A proteins in cell migration.

CDC50A is the beta-subunit of ATP8A2 and is crucial for the correct folding, stable expression, export from endoplasmic reticulum, and phosphatidylserine flippase activity of ATP8A2
Title: Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.

Import of choline phospholipids into Saccharomyces cerevisiae DeltaLem3 vector is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing that the proteins are orthologous.
Title: Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells.

The following TMEM30A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM30A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01587	NM_001143958.2 Latest version!	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu08223	NM_018247.4 Latest version!	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
View Old Accession Versions >>
OHu08223	NM_018247.3	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
OHu01587	NM_001143958.1	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM30A ( NM_001143958.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137430.1 Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01587
Clone ID Related Accession (Same CDS sequence)	NM_001143958.1 , NM_001143958.2
Accession Version	NM_001143958.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	cell cycle control protein 50A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA217148.1, DB239737.1, AK292823.1, AL832490.1, AL080250.11 and AA599967.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.55511.1, SRR1803614.142625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGGGC 
AACGTGTTTA TGTATTATGG ACTGTCTAAT TTCTATCAAA ACCATCGTCG TTACGTGAAA 
TCTCGAGATG ATAGTCAACT AAATGGAGAT TCTAGTGCTT TGCTTAATCC CAGTAAGGAA 
TGTGAACCTT ATCGAAGAAA TGAAGACAAA CCAATTGCTC CTTGTGGAGC TATTGCCAAC 
AGCATGTTTA ATGATACATT AGAATTGTTT CTCATTGGCA ATGATTCTTA TCCTATACCT 
ATCGCTTTGA AAAAGAAAGG TATTGCTTGG TGGACAGATA AAAATGTGAA ATTCAGAAAT 
CCCCCTGGAG GAGACAACCT GGAAGAACGA TTTAAAGGTA CAACAAAGCC TGTGAACTGG 
CTTAAACCAG TTTACATGCT GGATTCTGAC CCAGATAATA ATGGATTCAT AAATGAGGAT 
TTTATTGTTT GGATGCGTAC TGCAGCATTA CCTACTTTTC GCAAGTTGTA TCGTCTTATA 
GAAAGGAAAA GTGATTTACA TCCAACATTA CCAGCTGGCC GATACTCTTT GAATGTCACA 
TACAATTACC CTGTACATTA TTTTGATGGA CGAAAACGGA TGATCTTGAG CACTATTTCA 
TGGATGGGAG GAAAAAATCC ATTTTTGGGG ATTGCTTACA TCGCTGTTGG ATCCATCTCC 
TTCCTTCTGG GAGTTGTACT GCTAGTAATT AATCATAAAT ATAGAAACAG TAGTAATACA 
GCTGACATTA CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137430.1
CDS	279..1256
Translation	MAMNYNAKDEVDGGPPCAPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPIFFIIGLIFIPIGIGIFVTSNNIREIEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSSALLNPSKECEPYRRNEDKPIAPCGAIANSMFNDTLELFLIGNDSYPIPIALKKKGIAWWTDKNVKFRNPPGGDNLEERFKGTTKPVNWLKPVYMLDSDPDNNGFINEDFIVWMRTAALPTFRKLYRLIERKSDLHPTLPAGRYSLNVTYNYPVHYFDGRKRMILSTISWMGGKNPFLGIAYIAVGSISFLLGVVLLVINHKYRNSSNTADITI

Target ORF information:

RefSeq Version	NM_001143958.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143958.1

ORF Insert Sequence:

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGGGC 
AACGTGTTTA TGTATTATGG ACTGTCTAAT TTCTATCAAA ACCATCGTCG TTACGTGAAA 
TCTCGAGATG ATAGTCAACT AAATGGAGAT TCTAGTGCTT TGCTTAATCC CAGTAAGGAA 
TGTGAACCTT ATCGAAGAAA TGAAGACAAA CCAATTGCTC CTTGTGGAGC TATTGCCAAC 
AGCATGTTTA ATGATACATT AGAATTGTTT CTCATTGGCA ATGATTCTTA TCCTATACCT 
ATCGCTTTGA AAAAGAAAGG TATTGCTTGG TGGACAGATA AAAATGTGAA ATTCAGAAAT 
CCCCCTGGAG GAGACAACCT GGAAGAACGA TTTAAAGGTA CAACAAAGCC TGTGAACTGG 
CTTAAACCAG TTTACATGCT GGATTCTGAC CCAGATAATA ATGGATTCAT AAATGAGGAT 
TTTATTGTTT GGATGCGTAC TGCAGCATTA CCTACTTTTC GCAAGTTGTA TCGTCTTATA 
GAAAGGAAAA GTGATTTACA TCCAACATTA CCAGCTGGCC GATACTCTTT GAATGTCACA 
TACAATTACC CTGTACATTA TTTTGATGGA CGAAAACGGA TGATCTTGAG CACTATTTCA 
TGGATGGGAG GAAAAAATCC ATTTTTGGGG ATTGCTTACA TCGCTGTTGG ATCCATCTCC 
TTCCTTCTGG GAGTTGTACT GCTAGTAATT AATCATAAAT ATAGAAACAG TAGTAATACA 
GCTGACATTA CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM30A ( NM_001143958.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137430.1 Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01587
Clone ID Related Accession (Same CDS sequence)	NM_001143958.1 , NM_001143958.2
Accession Version	NM_001143958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	cell cycle control protein 50A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB239737.1, AK292823.1, AL832490.1, AL080250.11 and AA599967.1. On May 31, 2019 this sequence version replaced NM_001143958.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.246239.1, SRR1803616.55511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGGGC 
AACGTGTTTA TGTATTATGG ACTGTCTAAT TTCTATCAAA ACCATCGTCG TTACGTGAAA 
TCTCGAGATG ATAGTCAACT AAATGGAGAT TCTAGTGCTT TGCTTAATCC CAGTAAGGAA 
TGTGAACCTT ATCGAAGAAA TGAAGACAAA CCAATTGCTC CTTGTGGAGC TATTGCCAAC 
AGCATGTTTA ATGATACATT AGAATTGTTT CTCATTGGCA ATGATTCTTA TCCTATACCT 
ATCGCTTTGA AAAAGAAAGG TATTGCTTGG TGGACAGATA AAAATGTGAA ATTCAGAAAT 
CCCCCTGGAG GAGACAACCT GGAAGAACGA TTTAAAGGTA CAACAAAGCC TGTGAACTGG 
CTTAAACCAG TTTACATGCT GGATTCTGAC CCAGATAATA ATGGATTCAT AAATGAGGAT 
TTTATTGTTT GGATGCGTAC TGCAGCATTA CCTACTTTTC GCAAGTTGTA TCGTCTTATA 
GAAAGGAAAA GTGATTTACA TCCAACATTA CCAGCTGGCC GATACTCTTT GAATGTCACA 
TACAATTACC CTGTACATTA TTTTGATGGA CGAAAACGGA TGATCTTGAG CACTATTTCA 
TGGATGGGAG GAAAAAATCC ATTTTTGGGG ATTGCTTACA TCGCTGTTGG ATCCATCTCC 
TTCCTTCTGG GAGTTGTACT GCTAGTAATT AATCATAAAT ATAGAAACAG TAGTAATACA 
GCTGACATTA CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137430.1
CDS	155..1132
Translation	MAMNYNAKDEVDGGPPCAPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPIFFIIGLIFIPIGIGIFVTSNNIREIEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSSALLNPSKECEPYRRNEDKPIAPCGAIANSMFNDTLELFLIGNDSYPIPIALKKKGIAWWTDKNVKFRNPPGGDNLEERFKGTTKPVNWLKPVYMLDSDPDNNGFINEDFIVWMRTAALPTFRKLYRLIERKSDLHPTLPAGRYSLNVTYNYPVHYFDGRKRMILSTISWMGGKNPFLGIAYIAVGSISFLLGVVLLVINHKYRNSSNTADITI

Target ORF information:

RefSeq Version	NM_001143958.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143958.2

ORF Insert Sequence:

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGGGC 
AACGTGTTTA TGTATTATGG ACTGTCTAAT TTCTATCAAA ACCATCGTCG TTACGTGAAA 
TCTCGAGATG ATAGTCAACT AAATGGAGAT TCTAGTGCTT TGCTTAATCC CAGTAAGGAA 
TGTGAACCTT ATCGAAGAAA TGAAGACAAA CCAATTGCTC CTTGTGGAGC TATTGCCAAC 
AGCATGTTTA ATGATACATT AGAATTGTTT CTCATTGGCA ATGATTCTTA TCCTATACCT 
ATCGCTTTGA AAAAGAAAGG TATTGCTTGG TGGACAGATA AAAATGTGAA ATTCAGAAAT 
CCCCCTGGAG GAGACAACCT GGAAGAACGA TTTAAAGGTA CAACAAAGCC TGTGAACTGG 
CTTAAACCAG TTTACATGCT GGATTCTGAC CCAGATAATA ATGGATTCAT AAATGAGGAT 
TTTATTGTTT GGATGCGTAC TGCAGCATTA CCTACTTTTC GCAAGTTGTA TCGTCTTATA 
GAAAGGAAAA GTGATTTACA TCCAACATTA CCAGCTGGCC GATACTCTTT GAATGTCACA 
TACAATTACC CTGTACATTA TTTTGATGGA CGAAAACGGA TGATCTTGAG CACTATTTCA 
TGGATGGGAG GAAAAAATCC ATTTTTGGGG ATTGCTTACA TCGCTGTTGG ATCCATCTCC 
TTCCTTCTGG GAGTTGTACT GCTAGTAATT AATCATAAAT ATAGAAACAG TAGTAATACA 
GCTGACATTA CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM30A ( NM_018247.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060717.1 Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08223
Clone ID Related Accession (Same CDS sequence)	NM_018247.4 , NM_018247.3
Accession Version	NM_018247.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	cell cycle control protein 50A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB239737.1, AK001718.1, AJ420517.1, AL080250.11 and AA599967.1. On Nov 24, 2018 this sequence version replaced NM_018247.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832815.1, SRR1803615.207327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000230461.11/ ENSP00000230461.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGAGCC TTCCAGTCCC TGTAATAAAT GTTTATCTCC GGATGTGACA 
CCTTGCTTTT GTACCATTAA CTTCACACTG GAAAAGTCAT TTGAGGGCAA CGTGTTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGTT ACGTGAAATC TCGAGATGAT 
AGTCAACTAA ATGGAGATTC TAGTGCTTTG CTTAATCCCA GTAAGGAATG TGAACCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGAGCTA TTGCCAACAG CATGTTTAAT 
GATACATTAG AATTGTTTCT CATTGGCAAT GATTCTTATC CTATACCTAT CGCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTGAAAT TCAGAAATCC CCCTGGAGGA 
GACAACCTGG AAGAACGATT TAAAGGTACA ACAAAGCCTG TGAACTGGCT TAAACCAGTT 
TACATGCTGG ATTCTGACCC AGATAATAAT GGATTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGC AAGTTGTATC GTCTTATAGA AAGGAAAAGT 
GATTTACATC CAACATTACC AGCTGGCCGA TACTCTTTGA ATGTCACATA CAATTACCCT 
GTACATTATT TTGATGGACG AAAACGGATG ATCTTGAGCA CTATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC GCTGTTGGAT CCATCTCCTT CCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACAGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060717.1
CDS	155..1240
Translation	MAMNYNAKDEVDGGPPCAPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPIFFIIGLIFIPIGIGIFVTSNNIREIEIDYTGTEPSSPCNKCLSPDVTPCFCTINFTLEKSFEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSSALLNPSKECEPYRRNEDKPIAPCGAIANSMFNDTLELFLIGNDSYPIPIALKKKGIAWWTDKNVKFRNPPGGDNLEERFKGTTKPVNWLKPVYMLDSDPDNNGFINEDFIVWMRTAALPTFRKLYRLIERKSDLHPTLPAGRYSLNVTYNYPVHYFDGRKRMILSTISWMGGKNPFLGIAYIAVGSISFLLGVVLLVINHKYRNSSNTADITI

Target ORF information:

RefSeq Version	NM_018247.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018247.4

ORF Insert Sequence:

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGAGCC TTCCAGTCCC TGTAATAAAT GTTTATCTCC GGATGTGACA 
CCTTGCTTTT GTACCATTAA CTTCACACTG GAAAAGTCAT TTGAGGGCAA CGTGTTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGTT ACGTGAAATC TCGAGATGAT 
AGTCAACTAA ATGGAGATTC TAGTGCTTTG CTTAATCCCA GTAAGGAATG TGAACCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGAGCTA TTGCCAACAG CATGTTTAAT 
GATACATTAG AATTGTTTCT CATTGGCAAT GATTCTTATC CTATACCTAT CGCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTGAAAT TCAGAAATCC CCCTGGAGGA 
GACAACCTGG AAGAACGATT TAAAGGTACA ACAAAGCCTG TGAACTGGCT TAAACCAGTT 
TACATGCTGG ATTCTGACCC AGATAATAAT GGATTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGC AAGTTGTATC GTCTTATAGA AAGGAAAAGT 
GATTTACATC CAACATTACC AGCTGGCCGA TACTCTTTGA ATGTCACATA CAATTACCCT 
GTACATTATT TTGATGGACG AAAACGGATG ATCTTGAGCA CTATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC GCTGTTGGAT CCATCTCCTT CCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACAGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM30A ( NM_018247.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060717.1 Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08223
Clone ID Related Accession (Same CDS sequence)	NM_018247.4 , NM_018247.3
Accession Version	NM_018247.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cell cycle control protein 50A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA217148.1, DB239737.1, AK001718.1, AJ420517.1, AL080250.11 and AA599967.1. On Jan 23, 2009 this sequence version replaced NM_018247.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.123838.1, SRR1803612.93521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGAGCC TTCCAGTCCC TGTAATAAAT GTTTATCTCC GGATGTGACA 
CCTTGCTTTT GTACCATTAA CTTCACACTG GAAAAGTCAT TTGAGGGCAA CGTGTTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGTT ACGTGAAATC TCGAGATGAT 
AGTCAACTAA ATGGAGATTC TAGTGCTTTG CTTAATCCCA GTAAGGAATG TGAACCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGAGCTA TTGCCAACAG CATGTTTAAT 
GATACATTAG AATTGTTTCT CATTGGCAAT GATTCTTATC CTATACCTAT CGCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTGAAAT TCAGAAATCC CCCTGGAGGA 
GACAACCTGG AAGAACGATT TAAAGGTACA ACAAAGCCTG TGAACTGGCT TAAACCAGTT 
TACATGCTGG ATTCTGACCC AGATAATAAT GGATTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGC AAGTTGTATC GTCTTATAGA AAGGAAAAGT 
GATTTACATC CAACATTACC AGCTGGCCGA TACTCTTTGA ATGTCACATA CAATTACCCT 
GTACATTATT TTGATGGACG AAAACGGATG ATCTTGAGCA CTATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC GCTGTTGGAT CCATCTCCTT CCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACAGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060717.1
CDS	279..1364
Translation	MAMNYNAKDEVDGGPPCAPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPIFFIIGLIFIPIGIGIFVTSNNIREIEIDYTGTEPSSPCNKCLSPDVTPCFCTINFTLEKSFEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSSALLNPSKECEPYRRNEDKPIAPCGAIANSMFNDTLELFLIGNDSYPIPIALKKKGIAWWTDKNVKFRNPPGGDNLEERFKGTTKPVNWLKPVYMLDSDPDNNGFINEDFIVWMRTAALPTFRKLYRLIERKSDLHPTLPAGRYSLNVTYNYPVHYFDGRKRMILSTISWMGGKNPFLGIAYIAVGSISFLLGVVLLVINHKYRNSSNTADITI

Target ORF information:

RefSeq Version	NM_018247.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 30A (TMEM30A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018247.3

ORF Insert Sequence:

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGTG GGCCCCCGTG TGCTCCGGGG 
GGCACCGCGA AGACTCGGAG ACCGGATAAC ACGGCCTTCA AACAGCAACG GCTGCCAGCT 
TGGCAGCCCA TCCTTACGGC TGGCACGGTG CTACCTATTT TCTTCATCAT CGGTCTCATC 
TTCATTCCCA TCGGCATTGG CATTTTTGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGAGCC TTCCAGTCCC TGTAATAAAT GTTTATCTCC GGATGTGACA 
CCTTGCTTTT GTACCATTAA CTTCACACTG GAAAAGTCAT TTGAGGGCAA CGTGTTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGTT ACGTGAAATC TCGAGATGAT 
AGTCAACTAA ATGGAGATTC TAGTGCTTTG CTTAATCCCA GTAAGGAATG TGAACCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGAGCTA TTGCCAACAG CATGTTTAAT 
GATACATTAG AATTGTTTCT CATTGGCAAT GATTCTTATC CTATACCTAT CGCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTGAAAT TCAGAAATCC CCCTGGAGGA 
GACAACCTGG AAGAACGATT TAAAGGTACA ACAAAGCCTG TGAACTGGCT TAAACCAGTT 
TACATGCTGG ATTCTGACCC AGATAATAAT GGATTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGC AAGTTGTATC GTCTTATAGA AAGGAAAAGT 
GATTTACATC CAACATTACC AGCTGGCCGA TACTCTTTGA ATGTCACATA CAATTACCCT 
GTACATTATT TTGATGGACG AAAACGGATG ATCTTGAGCA CTATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC GCTGTTGGAT CCATCTCCTT CCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACAGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane.
The Journal of biological chemistry(2017 Dec)
Segawa K,Kurata S,Nagata S

The lipid flippase heterodimer ATP8B1-CDC50A is essential for surface expression of the apical sodium-dependent bile acid transporter (SLC10A2/ASBT) in intestinal Caco-2 cells.
Biochimica et biophysica acta1842(12 Pt A)2378-86(2014 Dec)
van der Mark VA,de Waart DR,Ho-Mok KS,Tabbers MM,Voogt HW,Oude Elferink RP,Knisely AS,Paulusma CC

Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure.
Science (New York, N.Y.)344(6188)1164-8(2014 Jun)
Segawa K,Kurata S,Yanagihashi Y,Brummelkamp TR,Matsuda F,Nagata S

Role for phospholipid flippase complex of ATP8A1 and CDC50A proteins in cell migration.
The Journal of biological chemistry288(7)4922-34(2013 Feb)
Kato U,Inadome H,Yamamoto M,Emoto K,Kobayashi T,Umeda M

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

TMEM30A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報