TMEM30A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM30A
Entrez Gene ID	505797
Full Name	transmembrane protein 30A
General protein information	Preferred Names transmembrane protein 30A Names cell cycle control protein 50A P4-ATPase flippase complex beta subunit TMEM30A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001075223.1	NP_001068691.1	cell cycle control protein 50A

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G54320	NP_564656.1
Oryza sativa (rice)	Os06g0665000	NP_001058302.1
Canis lupus familiaris (dog)	TMEM30A	XP_867464.3
Saccharomyces cerevisiae (baker's yeast)	YNR048W	NP_014446.3
Schizosaccharomyces pombe (fission yeast)	mug89	NP_595126.1
Magnaporthe oryzae (rice blast fungus)	MGG_12405	XP_003715571.1
	TMEM30A	XP_001109918.2
Caenorhabditis elegans (roundworm)	chat-1	NP_001023332.1
Arabidopsis thaliana (thale cress)	AT1G16360	NP_173086.2
Xenopus tropicalis (tropical clawed frog)	tmem30a	NP_989133.1
Bos taurus (cattle)	TMEM30A	NP_001068691.1
Mus musculus (house mouse)	Tmem30a	NP_598479.1
Gallus gallus (chicken)	TMEM30A	NP_001012897.1
Homo sapiens (human)	TMEM30A	NP_060717.1
Pan troglodytes (chimpanzee)	TMEM30A	XP_001143816.1
Rattus norvegicus (Norway rat)	Tmem30a	NP_001004248.1
Drosophila melanogaster (fruit fly)	CG9947	NP_573128.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000704	XP_311234.5
Neurospora crassa	NCU01165	XP_961531.1
Arabidopsis thaliana (thale cress)	ALIS5	NP_565210.1
Arabidopsis thaliana (thale cress)	ALIS1	NP_566435.1
Oryza sativa (rice)	Os02g0173800	NP_001046043.1

Gene Ontology
Bibliography

Related articles in PubMed

Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.
Vestergaard AL, Mikkelsen SA, Coleman JA, Molday RS, Vilsen B, Andersen JP
FEBS letters589(24 Pt B)3908-14(2015 Dec)

Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.
Coleman JA, Molday RS
The Journal of biological chemistry286(19)17205-16(2011 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit
Title: Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.

CDC50A is the beta-subunit of ATP8A2 and is crucial for the correct folding, stable expression, export from endoplasmic reticulum, and phosphatidylserine flippase activity of ATP8A2
Title: Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.

The following TMEM30A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM30A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37317	NM_001075223.1 Latest version!	Bos taurus transmembrane protein 30A (TMEM30A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM30A ( NM_001075223.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068691.1 Bos taurus transmembrane protein 30A (TMEM30A), mRNA.

Price & Availability↑

CloneID	OBa37317
Clone ID Related Accession (Same CDS sequence)	NM_001075223.1
Accession Version	NM_001075223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	cell cycle control protein 50A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC118287.1. On Oct 4, 2006 this sequence version replaced XM_582142.2. ##Evidence-Data-START## Transcript exon combination :: BC118287.1, DV893226.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGCG GGCCCCCGTG TCCTCCCGGG 
GGCACCGCTA AGACCCGGAG ACCAGATAAC ACGGCCTTCA AACAGCAACG GCTGCCGGCT 
TGGCAGCCCA TCCTGACGGC TGGCACGGTG CTGCCCACCT TCTTCATCAT CGGCCTCATC 
TTCATCCCCA TTGGCATCGG CATCTTCGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGATCC TTCCAGTCCC TGTAATAAAT GTTTGTCTCC GAATGTTACA 
CCTTGTGTTT GTACCATTAA CTTCACACTG GAACAGTCAT TTGAGGGCAA TGTATTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGCT ATGTGAAATC GCGAGATGAT 
GGTCAGCTAA ATGGAGATCC TAGTGCTTTG CTTAATCCCA GTAAGGAGTG TGAGCCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGGGCTA TAGCCAACAG CATGTTTAAT 
GATACACTAG AATTGTTTCA GGTTGGCAAT GCATCTGATC TTACGCCTAT TCCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTAAAAT TCAGAAATCC CCCTGGAACA 
GATCCCTTAG AAGAACGATT CAAAGGCACA ACAAAGCCAG TAAACTGGGT TAAACCAGTT 
TACATGTTGG ATTCTGACGA AGATAATAAT GGCTTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGT AAGTTATATC GTCTTATAGA GCGGAAAAAT 
GATTTACATC CAACATTACC AGCTGGACGA TACTATTTGA ATATCACATA CAATTACCCT 
GTACATTCAT TTGATGGACG GAAGCGGATG ATCTTGAGCA CCATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC ACTATTGGAT CCATCTCCTT TCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACTGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068691.1
CDS	103..1188
Translation	MAMNYNAKDEVDGGPPCPPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPTFFIIGLIFIPIGIGIFVTSNNIREIEIDYTGTDPSSPCNKCLSPNVTPCVCTINFTLEQSFEGNVFMYYGLSNFYQNHRRYVKSRDDGQLNGDPSALLNPSKECEPYRRNEDKPIAPCGAIANSMFNDTLELFQVGNASDLTPIPLKKKGIAWWTDKNVKFRNPPGTDPLEERFKGTTKPVNWVKPVYMLDSDEDNNGFINEDFIVWMRTAALPTFRKLYRLIERKNDLHPTLPAGRYYLNITYNYPVHSFDGRKRMILSTISWMGGKNPFLGIAYITIGSISFLLGVVLLVINHKYRNSSNTADITI

Target ORF information:

RefSeq Version	NM_001075223.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 30A (TMEM30A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075223.1

ORF Insert Sequence:

ATGGCGATGA ACTATAACGC GAAGGATGAA GTGGACGGCG GGCCCCCGTG TCCTCCCGGG 
GGCACCGCTA AGACCCGGAG ACCAGATAAC ACGGCCTTCA AACAGCAACG GCTGCCGGCT 
TGGCAGCCCA TCCTGACGGC TGGCACGGTG CTGCCCACCT TCTTCATCAT CGGCCTCATC 
TTCATCCCCA TTGGCATCGG CATCTTCGTC ACCTCCAACA ACATCCGCGA GATCGAGATT 
GATTATACCG GAACAGATCC TTCCAGTCCC TGTAATAAAT GTTTGTCTCC GAATGTTACA 
CCTTGTGTTT GTACCATTAA CTTCACACTG GAACAGTCAT TTGAGGGCAA TGTATTTATG 
TATTATGGAC TGTCTAATTT CTATCAAAAC CATCGTCGCT ATGTGAAATC GCGAGATGAT 
GGTCAGCTAA ATGGAGATCC TAGTGCTTTG CTTAATCCCA GTAAGGAGTG TGAGCCTTAT 
CGAAGAAATG AAGACAAACC AATTGCTCCT TGTGGGGCTA TAGCCAACAG CATGTTTAAT 
GATACACTAG AATTGTTTCA GGTTGGCAAT GCATCTGATC TTACGCCTAT TCCTTTGAAA 
AAGAAAGGTA TTGCTTGGTG GACAGATAAA AATGTAAAAT TCAGAAATCC CCCTGGAACA 
GATCCCTTAG AAGAACGATT CAAAGGCACA ACAAAGCCAG TAAACTGGGT TAAACCAGTT 
TACATGTTGG ATTCTGACGA AGATAATAAT GGCTTCATAA ATGAGGATTT TATTGTTTGG 
ATGCGTACTG CAGCATTACC TACTTTTCGT AAGTTATATC GTCTTATAGA GCGGAAAAAT 
GATTTACATC CAACATTACC AGCTGGACGA TACTATTTGA ATATCACATA CAATTACCCT 
GTACATTCAT TTGATGGACG GAAGCGGATG ATCTTGAGCA CCATTTCATG GATGGGAGGA 
AAAAATCCAT TTTTGGGGAT TGCTTACATC ACTATTGGAT CCATCTCCTT TCTTCTGGGA 
GTTGTACTGC TAGTAATTAA TCATAAATAT AGAAACAGTA GTAATACTGC TGACATTACC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.
FEBS letters589(24 Pt B)3908-14(2015 Dec)
Vestergaard AL,Mikkelsen SA,Coleman JA,Molday RS,Vilsen B,Andersen JP

Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.
The Journal of biological chemistry286(19)17205-16(2011 May)
Coleman JA,Molday RS

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TMEM30A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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