SEPP1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEPP1B
Entrez Gene ID	791479
Full Name	selenoprotein P2
Synonyms	SePPb,SelPb,cb689,fb59b06,id:ibd5022,sePb,sepp1b,wu:fb59b06
General protein information	Preferred Names selenoprotein P2 Names selenoprotein Pb selenoprotein P, 1b selenoprotein P, plasma, 1b zSelPb
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)
Summary	This gene has a 5-exon structure similar to the paralogous selenoprotein Pa gene (GeneID:352931). These two genes are thought to have arisen by whole genome duplication. This gene encodes a vertebrate-specific selenoprotein that contains a single selenocysteine (Sec) residue compared to 17 in selenoprotein Pa. The exact function of this selenoprotein is not known; however, it is predicted to have a signal peptide, so it is probably secreted like selenoprotein Pa, and may function as an extracellular antioxidant. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs in vertebrates contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains a single SECIS element compared to two in the mRNA for selenoprotein Pa. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001353911.1	NP_001340840.1	selenoprotein Pb precursor
NM_178298.3	NP_840083.2	selenoprotein Pb precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sepp1b	NP_840083.2
Xenopus tropicalis (tropical clawed frog)	sepp1-like	NP_001006908.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of Selenoprotein P cDNA of the Antarctic toothfish Dissostichus mawsoni and its role under cold pressure.
Li Y, Luo J, Xu Q, Hou Y, Jiang P, Sun Y, Lu H, Han B, Zhang J
Gene(2018 Jan)

Re-evaluating the functional landscape of the cardiovascular system during development.
Takada N, Omae M, Sagawa F, Chi NC, Endo S, Kozawa S, Sato TN
Biology open6(11)1756-1770(2017 Nov)

Wu J, Choi TY, Shin D
Gene expression17(3)237-249(2017 Jul)

Transcriptional and Behavioral Responses of Zebrafish Larvae to Microcystin-LR Exposure.
Tzima E, Serifi I, Tsikari I, Alzualde A, Leonardos I, Papamarcaki T
International journal of molecular sciences18(2)(2017 Feb)

MiR-145 mediates zebrafish hepatic outgrowth through progranulin A signaling.
Li YW, Chiang KY, Li YH, Wu SY, Liu W, Lin CR, Wu JL
PloS one12(5)e0177887(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SEPP1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEPP1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa141154	NM_001353911.1 Latest version!	Danio rerio selenoprotein P2 (selenop2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
ODa00595	NM_178298.3 Latest version!	Danio rerio selenoprotein P, plasma, 1b (sepp1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEPP1B ( NM_001353911.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001340840.1 Danio rerio selenoprotein P2 (selenop2), mRNA.

Price & Availability↑

CloneID	ODa141154
Clone ID Related Accession (Same CDS sequence)	NM_001353911.1
Accession Version	NM_001353911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Danio rerio(zebrafish)
Product	selenoprotein Pb precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH498018.1, EH593654.1 and EH518168.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086844.1, AF322072.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 11168591 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGCTC TGTGGCCTTT GCTGCTGTCT GCTTTTCCGG CTTTGCTGGG AGCTTCATCA 
CTGTTTGTGG AAAAGGAAAG CAGTGGTTCG AGGATCTGTA AACCTGCCCC GCAGTGGGAG 
ATCGATGGCA AGACTCCCAT GAAGGAACTT CTGGGAAATG TAGTCGTCGT AGCTCTGCTT 
AAAGCCAGCT GACATTTCTG TCTGACGCAG GCAGCCAGGC TGGGAGACCT GCGTGACAAG 
CTGGCTAATG GAGGACTGAC TAACATTTCA TTCATGGTTG TGAATGAGCA GGACTCTCAG 
TCCAGAGCCA TGTACTGGGA GCTGAAAAGG AGGACAGCTC AGGACATCCC GGTGTACCAG 
CAGAGCCCAC TGCAAAATGA CGTCTGGGAG ATCCTGGAGG GAGACAAGGA TGATTTCTTA 
GTGTATGACA GATGTGGATA CCTCACCTTT CACATTGTCC TGCCATTCAG TTTTCTTCAC 
TACCCTTATA TAGAAGCTGC CATCAGGGCT ACATATCACA AAAACATGTG CAACTGCTCA 
CTAAATGCCA ACTTTTCCAT ATCAGAAAGC TCTGACAGCA CCAAAAATGA GCCCGCAGGA 
GAAAACAACC AGCGGCCCAA CAGTACAGAG CCAGTAACTG CTGCTCATCA TCATCATCAT 
CAACAACATG AGCCTCATCA TCATCATCAT AATCCATATC CAAATAGCCA TAAAAAATCA 
GGCGATTCAG ATGTGACCGG GAAACCAAAG GAGCCAACCC ATCATTCTCA TCAAGAACAT 
GTTCACAACC ACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340840.1
CDS	107..904
Translation	MQALWPLLLSAFPALLGASSLFVEKESSGSRICKPAPQWEIDGKTPMKELLGNVVVVALLKAS*HFCLTQAARLGDLRDKLANGGLTNISFMVVNEQDSQSRAMYWELKRRTAQDIPVYQQSPLQNDVWEILEGDKDDFLVYDRCGYLTFHIVLPFSFLHYPYIEAAIRATYHKNMCNCSLNANFSISESSDSTKNEPAGENNQRPNSTEPVTAAHHHHHQQHEPHHHHHNPYPNSHKKSGDSDVTGKPKEPTHHSHQEHVHNHR

Target ORF information:

RefSeq Version	NM_001353911.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein P2 (selenop2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353911.1

ORF Insert Sequence:

ATGCAGGCTC TGTGGCCTTT GCTGCTGTCT GCTTTTCCGG CTTTGCTGGG AGCTTCATCA 
CTGTTTGTGG AAAAGGAAAG CAGTGGTTCG AGGATCTGTA AACCTGCCCC GCAGTGGGAG 
ATCGATGGCA AGACTCCCAT GAAGGAACTT CTGGGAAATG TAGTCGTCGT AGCTCTGCTT 
AAAGCCAGCT GACATTTCTG TCTGACGCAG GCAGCCAGGC TGGGAGACCT GCGTGACAAG 
CTGGCTAATG GAGGACTGAC TAACATTTCA TTCATGGTTG TGAATGAGCA GGACTCTCAG 
TCCAGAGCCA TGTACTGGGA GCTGAAAAGG AGGACAGCTC AGGACATCCC GGTGTACCAG 
CAGAGCCCAC TGCAAAATGA CGTCTGGGAG ATCCTGGAGG GAGACAAGGA TGATTTCTTA 
GTGTATGACA GATGTGGATA CCTCACCTTT CACATTGTCC TGCCATTCAG TTTTCTTCAC 
TACCCTTATA TAGAAGCTGC CATCAGGGCT ACATATCACA AAAACATGTG CAACTGCTCA 
CTAAATGCCA ACTTTTCCAT ATCAGAAAGC TCTGACAGCA CCAAAAATGA GCCCGCAGGA 
GAAAACAACC AGCGGCCCAA CAGTACAGAG CCAGTAACTG CTGCTCATCA TCATCATCAT 
CAACAACATG AGCCTCATCA TCATCATCAT AATCCATATC CAAATAGCCA TAAAAAATCA 
GGCGATTCAG ATGTGACCGG GAAACCAAAG GAGCCAACCC ATCATTCTCA TCAAGAACAT 
GTTCACAACC ACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEPP1B ( NM_178298.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_840083.2 Danio rerio selenoprotein P, plasma, 1b (sepp1b), mRNA.

Price & Availability↑

CloneID	ODa00595
Clone ID Related Accession (Same CDS sequence)	NM_178298.3
Accession Version	NM_178298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-24
Organism	Danio rerio(Zebrafish)
Product	selenoprotein Pb precursor
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH498018.1, EH527207.1 and EH518168.1. On Dec 4, 2010 this sequence version replaced NM_178298.2. ##Evidence-Data-START## Transcript exon combination :: EH498018.1, BC086844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA708828, SAMEA898401 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 11168591 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGCTC TGTGGCCTTT GCTGCTGTCT GCTTTTCCGG CTTTGCTGGG AGCTTCATCA 
CTGTTTGTGG AAAAGGAAAG CAGTGGTTCG AGGATCTGTA AACCTGCCCC GCAGTGGGAG 
ATCGATGGCA AGACTCCCAT GAAGGAACTT CTGGGAAATG TAGTCGTCGT AGCTCTGCTT 
AAAGCCAGCT GACATTTCTG TCTGACGCAG GCAGCCAGGC TGGGAGACCT GCGTGACAAG 
CTGGCTAATG GAGGACTGAC TAACATTTCA TTCATGGTTG TGAATGAGCA GGACTCTCAG 
TCCAGAGCCA TGTACTGGGA GCTGAAAAGG AGGACAGCTC AGGACATCCC GGTGTACCAG 
CAGAGCCCAC TGCAAAATGA CGTCTGGGAG ATCCTGGAGG GAGACAAGGA TGATTTCTTA 
GTGTATGACA GATGTGGATA CCTCACCTTT CACATTGTCC TGCCATTCAG TTTTCTTCAC 
TACCCTTATA TAGAAGCTGC CATCAGGGCT ACATATCACA AAAACATGTG CAACTGCTCA 
CTAAATGCCA ACTTTTCCAT ATCAGAAAGC TCTGACAGCA CCAAAAATGA GCCCGCAGGA 
GAAAACAACC AGCGGCCCAA CAGTACAGAG CCAGTAACTG CTGCTCATCA TCATCATCAT 
CAACAACATG AGCCTCATCA TCATCATAAT CCATATCCAA ATAACCATAA AAAATCAGGC 
GATTCAGATG TGACCGGGAA ACCAAAGGAG CCAACCCATC ATTCTCATCA AGAACATGTT 
CACAACCACA GATTTTACAC AGTTTTTAGT TTTATTATTT TATTTTATAT AATGTTTTTT 
TTTTTTGTAA ATATATGGAG CTGCTCTTGT GTCAGAACTT CATATTTACC TCAGAGGGGG 
GTATTCGGTT GTCTTCAGTG TATTTTTGTT CTTAATGAAG TGTACACTTT AAACCTGAGA 
CAGGGGTCTG TATGCGGATG CTGAACACAG GAGTCATTTA TTTACACTAA ACTTCTGATT 
TCCCCCCTTT TTGGGAAAAT GTGATTGTGC TGAACTAAGT GGTTGCTTGG ATTATTATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840083.2
CDS	107..1189
Translation	MQALWPLLLSAFPALLGASSLFVEKESSGSRICKPAPQWEIDGKTPMKELLGNVVVVALLKASHFCLTQAARLGDLRDKLANGGLTNISFMVVNEQDSQSRAMYWELKRRTAQDIPVYQQSPLQNDVWEILEGDKDDFLVYDRCGYLTFHIVLPFSFLHYPYIEAAIRATYHKNMCNCSLNANFSISESSDSTKNEPAGENNQRPNSTEPVTAAHHHHHQQHEPHHHHNPYPNNHKKSGDSDVTGKPKEPTHHSHQEHVHNHRFYTVFSFIILFYIMFFFFVNIWSCSCVRTSYLPQRGVFGCLQCIFVLNEVYTLNLRQGSVCGCTQESFIYTKLLISPLFGKMLCTKWLLGLLC

Target ORF information:

RefSeq Version	NM_178298.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio selenoprotein P, plasma, 1b (sepp1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178298.3

ORF Insert Sequence:

ATGCAGGCTC TGTGGCCTTT GCTGCTGTCT GCTTTTCCGG CTTTGCTGGG AGCTTCATCA 
CTGTTTGTGG AAAAGGAAAG CAGTGGTTCG AGGATCTGTA AACCTGCCCC GCAGTGGGAG 
ATCGATGGCA AGACTCCCAT GAAGGAACTT CTGGGAAATG TAGTCGTCGT AGCTCTGCTT 
AAAGCCAGCT GACATTTCTG TCTGACGCAG GCAGCCAGGC TGGGAGACCT GCGTGACAAG 
CTGGCTAATG GAGGACTGAC TAACATTTCA TTCATGGTTG TGAATGAGCA GGACTCTCAG 
TCCAGAGCCA TGTACTGGGA GCTGAAAAGG AGGACAGCTC AGGACATCCC GGTGTACCAG 
CAGAGCCCAC TGCAAAATGA CGTCTGGGAG ATCCTGGAGG GAGACAAGGA TGATTTCTTA 
GTGTATGACA GATGTGGATA CCTCACCTTT CACATTGTCC TGCCATTCAG TTTTCTTCAC 
TACCCTTATA TAGAAGCTGC CATCAGGGCT ACATATCACA AAAACATGTG CAACTGCTCA 
CTAAATGCCA ACTTTTCCAT ATCAGAAAGC TCTGACAGCA CCAAAAATGA GCCCGCAGGA 
GAAAACAACC AGCGGCCCAA CAGTACAGAG CCAGTAACTG CTGCTCATCA TCATCATCAT 
CAACAACATG AGCCTCATCA TCATCATAAT CCATATCCAA ATAACCATAA AAAATCAGGC 
GATTCAGATG TGACCGGGAA ACCAAAGGAG CCAACCCATC ATTCTCATCA AGAACATGTT 
CACAACCACA GATTTTACAC AGTTTTTAGT TTTATTATTT TATTTTATAT AATGTTTTTT 
TTTTTTGTAA ATATATGGAG CTGCTCTTGT GTCAGAACTT CATATTTACC TCAGAGGGGG 
GTATTCGGTT GTCTTCAGTG TATTTTTGTT CTTAATGAAG TGTACACTTT AAACCTGAGA 
CAGGGGTCTG TATGCGGATG CTGAACACAG GAGTCATTTA TTTACACTAA ACTTCTGATT 
TCCCCCCTTT TTGGGAAAAT GTGATTGTGC TGAACTAAGT GGTTGCTTGG ATTATTATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of Selenoprotein P cDNA of the Antarctic toothfish Dissostichus mawsoni and its role under cold pressure.
Gene(2018 Jan)
Li Y,Luo J,Xu Q,Hou Y,Jiang P,Sun Y,Lu H,Han B,Zhang J

Re-evaluating the functional landscape of the cardiovascular system during development.
Biology open6(11)1756-1770(2017 Nov)
Takada N,Omae M,Sagawa F,Chi NC,Endo S,Kozawa S,Sato TN

Gene expression17(3)237-249(2017 Jul)
Wu J,Choi TY,Shin D

Transcriptional and Behavioral Responses of Zebrafish Larvae to Microcystin-LR Exposure.
International journal of molecular sciences18(2)(2017 Feb)
Tzima E,Serifi I,Tsikari I,Alzualde A,Leonardos I,Papamarcaki T

MiR-145 mediates zebrafish hepatic outgrowth through progranulin A signaling.
PloS one12(5)e0177887(2017)
Li YW,Chiang KY,Li YH,Wu SY,Liu W,Lin CR,Wu JL

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SEPP1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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