phka1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka1a
Entrez Gene ID	572183
Full Name	phosphorylase kinase, alpha 1a (muscle)
Synonyms	phka1,wu:fl37h01
General protein information	Preferred Names phosphorylase kinase, alpha 1a (muscle) Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform phosphorylase kinase, alpha 1 (muscle)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166519.4	XP_005166576.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_005166519.3	XP_005166576.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_017357095.1	XP_017212584.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_009303454.3	XP_009301729.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_009303454.2	XP_009301729.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_017357095.2	XP_017212584.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Gene Ontology

The following phka1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59784	XM_017357095.2 Latest version!	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa13437	XM_005166519.4 Latest version!	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13438	XM_009303454.3 Latest version!	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa13437	XM_005166519.3	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13438	XM_009303454.2	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa59784	XM_017357095.1	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka1a ( XM_017357095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212584.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.

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CloneID	ODa59784
Clone ID Related Accession (Same CDS sequence)	XM_017357095.1 , XM_017357095.2
Accession Version	XM_017357095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGATTCAG ATGATCTAGC ACAATACCTG GACCAGCTTT TGCATGCAGC GGTGCCTGTC 
CAGGGGCTCA GAGTCGAGGC AGAGGCTAAA GGACTGAGTC GCTTTAAGGC TGCAGCCACA 
AAGACACGTG AGATGGTGTC TCTTGTGAAC AAAGCCAAGG ATCTACAAAT CCACAATCTC 
CATATGTATC TTCCCAATAA GCTGTTCCGC TCACCTCAGC CTGCGCTTAA TTTAAAAGAC 
TTTCCAGGAC TGGGGACGGA TCATAGTTCA GAAGTTGTGG TGCCCTCTGA AAATAACCTG 
CCTCGGGACA CTCATGGTGC CATTGACTAC AGTAGTCTTG TGCAGGTCCT TAAAGACACT 
CAGAGCCTTC ACGATCAGGC AGACATCCTC TACATTCTTT TTAAAGACAA GGGAATGGAC 
TGGGACACTC AGCTGCATGG GAAAGGCACC ACTGTTAAGA GTTTGCTAGT CGAGCTCTAT 
GAGAAAGCGG GAGATCTGAG GTGTTGGGAT ATCATCCGTA TGATTTCAGG CATGCTCCGC 
AAGAAAGTAG AAGAACTAGA CTGGGCCTGC TCAGATTTGC TGGCTCATCA GAAGCACTTG 
ACGGTCGGTT TACCACCAGA GCCCAGAGAG AAAACCATTA CAGCGCCTTT GCCTCCTGAC 
CAACTGGCCT TACTGATTGA TCAAGCCAGC GAAAACAGCA TCACTATTGC CATCCTCACA 
CAGGAGATCA TAGTGTACCT AGCGATGATC ATCAGAACGC AGCGCAGTTT GTTCAGTGAG 
ATGTTTCGGC TGAGGATCGG CCTCATCATT CAGGTTATGG CAACTGAACT GGCCCAATCT 
CTAAACTGCT CAGGTGAGGA GGCCACAGAG AGTCTGATGA AACTGAGTCC CTCTGAGTTG 
AAGAATCTTC TTCACCACAT TCTGAGCGGA AAAGAATTCG GTGTTCAGAG AAGTGTTCGT 
TCAGTTGAGC CCGGTGTCAG TCCTGTCATC TCAATCCATG AGGTGGGCAA TGTTGGAGCC 
ACCAAGAGTG AGCGCGCAGG CATCAGCAAA CTGAAGAGTG ACATGAAGAT GCTGGAAGAG 
CTCAGATTTT CATGGTCGAT CCAAAGTAAG ACTTTCTCAG GTCAGTCCTT CGATGCTGAG 
CCTGTTGAAT TAGCGAGGAG CAGGATGCCT TCAATTGATG GGTTTGACAG CTCCGTGAGT 
GTTGGTGTAC CCCGGGATAG TCGGCAGGGC CAGTGGCTGC GCAGGAGGCG GCTTGATGGA 
GCTCTCAACA GAGTTCCTGT TGGCTTTTAT CAAAAAGTCT GGAAGATTCT GCAGAAGTGT 
CATGGCCTTT CAATAGAAGG CTTCGTTCTC CCATCTTCTA CTACAAGAGA GATGACCCCA 
GGTGAGATTA AATTTGCGGT ACATGTGGAA AGGGTGCTGA ACCGTGTCCC TCAGCCTGAA 
TACAGGCAGC TGCTGGTGGA AGGCATCCTC GTCTTGACAA TGCTGGCTGA TGTTGACATT 
CAGAGCATCG GCAGCATCAT CCACATAGAA AAAATAGTTC ATATTGCTAA TGACATGTTC 
TACAAAGACC AGAAGGATCT TGGTGCTGAG GACAGTATTT TGGAGAAGGA TCAGTCTACG 
GGCATCTGTC GGCTCCTGTA TGACACTGCA CCCAGCGGCC GCTTCGGCAC CATGACTTAC 
CTTACTAAAG CTGTGGCTAC TTATGCCCAG GACTTCTTGC CTAGTGGCGC CTGTACTGTA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212584.1
CDS	177..3782
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMLEELRFSWSIQSKTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_017357095.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357095.1

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGATTCAG ATGATCTAGC ACAATACCTG GACCAGCTTT TGCATGCAGC GGTGCCTGTC 
CAGGGGCTCA GAGTCGAGGC AGAGGCTAAA GGACTGAGTC GCTTTAAGGC TGCAGCCACA 
AAGACACGTG AGATGGTGTC TCTTGTGAAC AAAGCCAAGG ATCTACAAAT CCACAATCTC 
CATATGTATC TTCCCAATAA GCTGTTCCGC TCACCTCAGC CTGCGCTTAA TTTAAAAGAC 
TTTCCAGGAC TGGGGACGGA TCATAGTTCA GAAGTTGTGG TGCCCTCTGA AAATAACCTG 
CCTCGGGACA CTCATGGTGC CATTGACTAC AGTAGTCTTG TGCAGGTCCT TAAAGACACT 
CAGAGCCTTC ACGATCAGGC AGACATCCTC TACATTCTTT TTAAAGACAA GGGAATGGAC 
TGGGACACTC AGCTGCATGG GAAAGGCACC ACTGTTAAGA GTTTGCTAGT CGAGCTCTAT 
GAGAAAGCGG GAGATCTGAG GTGTTGGGAT ATCATCCGTA TGATTTCAGG CATGCTCCGC 
AAGAAAGTAG AAGAACTAGA CTGGGCCTGC TCAGATTTGC TGGCTCATCA GAAGCACTTG 
ACGGTCGGTT TACCACCAGA GCCCAGAGAG AAAACCATTA CAGCGCCTTT GCCTCCTGAC 
CAACTGGCCT TACTGATTGA TCAAGCCAGC GAAAACAGCA TCACTATTGC CATCCTCACA 
CAGGAGATCA TAGTGTACCT AGCGATGATC ATCAGAACGC AGCGCAGTTT GTTCAGTGAG 
ATGTTTCGGC TGAGGATCGG CCTCATCATT CAGGTTATGG CAACTGAACT GGCCCAATCT 
CTAAACTGCT CAGGTGAGGA GGCCACAGAG AGTCTGATGA AACTGAGTCC CTCTGAGTTG 
AAGAATCTTC TTCACCACAT TCTGAGCGGA AAAGAATTCG GTGTTCAGAG AAGTGTTCGT 
TCAGTTGAGC CCGGTGTCAG TCCTGTCATC TCAATCCATG AGGTGGGCAA TGTTGGAGCC 
ACCAAGAGTG AGCGCGCAGG CATCAGCAAA CTGAAGAGTG ACATGAAGAT GCTGGAAGAG 
CTCAGATTTT CATGGTCGAT CCAAAGTAAG ACTTTCTCAG GTCAGTCCTT CGATGCTGAG 
CCTGTTGAAT TAGCGAGGAG CAGGATGCCT TCAATTGATG GGTTTGACAG CTCCGTGAGT 
GTTGGTGTAC CCCGGGATAG TCGGCAGGGC CAGTGGCTGC GCAGGAGGCG GCTTGATGGA 
GCTCTCAACA GAGTTCCTGT TGGCTTTTAT CAAAAAGTCT GGAAGATTCT GCAGAAGTGT 
CATGGCCTTT CAATAGAAGG CTTCGTTCTC CCATCTTCTA CTACAAGAGA GATGACCCCA 
GGTGAGATTA AATTTGCGGT ACATGTGGAA AGGGTGCTGA ACCGTGTCCC TCAGCCTGAA 
TACAGGCAGC TGCTGGTGGA AGGCATCCTC GTCTTGACAA TGCTGGCTGA TGTTGACATT 
CAGAGCATCG GCAGCATCAT CCACATAGAA AAAATAGTTC ATATTGCTAA TGACATGTTC 
TACAAAGACC AGAAGGATCT TGGTGCTGAG GACAGTATTT TGGAGAAGGA TCAGTCTACG 
GGCATCTGTC GGCTCCTGTA TGACACTGCA CCCAGCGGCC GCTTCGGCAC CATGACTTAC 
CTTACTAAAG CTGTGGCTAC TTATGCCCAG GACTTCTTGC CTAGTGGCGC CTGTACTGTA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1a ( XM_017357095.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212584.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59784
Clone ID Related Accession (Same CDS sequence)	XM_017357095.1 , XM_017357095.2
Accession Version	XM_017357095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGATTCAG ATGATCTAGC ACAATACCTG GACCAGCTTT TGCATGCAGC GGTGCCTGTC 
CAGGGGCTCA GAGTCGAGGC AGAGGCTAAA GGACTGAGTC GCTTTAAGGC TGCAGCCACA 
AAGACACGTG AGATGGTGTC TCTTGTGAAC AAAGCCAAGG ATCTACAAAT CCACAATCTC 
CATATGTATC TTCCCAATAA GCTGTTCCGC TCACCTCAGC CTGCGCTTAA TTTAAAAGAC 
TTTCCAGGAC TGGGGACGGA TCATAGTTCA GAAGTTGTGG TGCCCTCTGA AAATAACCTG 
CCTCGGGACA CTCATGGTGC CATTGACTAC AGTAGTCTTG TGCAGGTCCT TAAAGACACT 
CAGAGCCTTC ACGATCAGGC AGACATCCTC TACATTCTTT TTAAAGACAA GGGAATGGAC 
TGGGACACTC AGCTGCATGG GAAAGGCACC ACTGTTAAGA GTTTGCTAGT CGAGCTCTAT 
GAGAAAGCGG GAGATCTGAG GTGTTGGGAT ATCATCCGTA TGATTTCAGG CATGCTCCGC 
AAGAAAGTAG AAGAACTAGA CTGGGCCTGC TCAGATTTGC TGGCTCATCA GAAGCACTTG 
ACGGTCGGTT TACCACCAGA GCCCAGAGAG AAAACCATTA CAGCGCCTTT GCCTCCTGAC 
CAACTGGCCT TACTGATTGA TCAAGCCAGC GAAAACAGCA TCACTATTGC CATCCTCACA 
CAGGAGATCA TAGTGTACCT AGCGATGATC ATCAGAACGC AGCGCAGTTT GTTCAGTGAG 
ATGTTTCGGC TGAGGATCGG CCTCATCATT CAGGTTATGG CAACTGAACT GGCCCAATCT 
CTAAACTGCT CAGGTGAGGA GGCCACAGAG AGTCTGATGA AACTGAGTCC CTCTGAGTTG 
AAGAATCTTC TTCACCACAT TCTGAGCGGA AAAGAATTCG GTGTTCAGAG AAGTGTTCGT 
TCAGTTGAGC CCGGTGTCAG TCCTGTCATC TCAATCCATG AGGTGGGCAA TGTTGGAGCC 
ACCAAGAGTG AGCGCGCAGG CATCAGCAAA CTGAAGAGTG ACATGAAGAT GCTGGAAGAG 
CTCAGATTTT CATGGTCGAT CCAAAGTAAG ACTTTCTCAG GTCAGTCCTT CGATGCTGAG 
CCTGTTGAAT TAGCGAGGAG CAGGATGCCT TCAATTGATG GGTTTGACAG CTCCGTGAGT 
GTTGGTGTAC CCCGGGATAG TCGGCAGGGC CAGTGGCTGC GCAGGAGGCG GCTTGATGGA 
GCTCTCAACA GAGTTCCTGT TGGCTTTTAT CAAAAAGTCT GGAAGATTCT GCAGAAGTGT 
CATGGCCTTT CAATAGAAGG CTTCGTTCTC CCATCTTCTA CTACAAGAGA GATGACCCCA 
GGTGAGATTA AATTTGCGGT ACATGTGGAA AGGGTGCTGA ACCGTGTCCC TCAGCCTGAA 
TACAGGCAGC TGCTGGTGGA AGGCATCCTC GTCTTGACAA TGCTGGCTGA TGTTGACATT 
CAGAGCATCG GCAGCATCAT CCACATAGAA AAAATAGTTC ATATTGCTAA TGACATGTTC 
TACAAAGACC AGAAGGATCT TGGTGCTGAG GACAGTATTT TGGAGAAGGA TCAGTCTACG 
GGCATCTGTC GGCTCCTGTA TGACACTGCA CCCAGCGGCC GCTTCGGCAC CATGACTTAC 
CTTACTAAAG CTGTGGCTAC TTATGCCCAG GACTTCTTGC CTAGTGGCGC CTGTACTGTA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212584.1
CDS	195..3800
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMLEELRFSWSIQSKTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_017357095.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357095.2

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGATTCAG ATGATCTAGC ACAATACCTG GACCAGCTTT TGCATGCAGC GGTGCCTGTC 
CAGGGGCTCA GAGTCGAGGC AGAGGCTAAA GGACTGAGTC GCTTTAAGGC TGCAGCCACA 
AAGACACGTG AGATGGTGTC TCTTGTGAAC AAAGCCAAGG ATCTACAAAT CCACAATCTC 
CATATGTATC TTCCCAATAA GCTGTTCCGC TCACCTCAGC CTGCGCTTAA TTTAAAAGAC 
TTTCCAGGAC TGGGGACGGA TCATAGTTCA GAAGTTGTGG TGCCCTCTGA AAATAACCTG 
CCTCGGGACA CTCATGGTGC CATTGACTAC AGTAGTCTTG TGCAGGTCCT TAAAGACACT 
CAGAGCCTTC ACGATCAGGC AGACATCCTC TACATTCTTT TTAAAGACAA GGGAATGGAC 
TGGGACACTC AGCTGCATGG GAAAGGCACC ACTGTTAAGA GTTTGCTAGT CGAGCTCTAT 
GAGAAAGCGG GAGATCTGAG GTGTTGGGAT ATCATCCGTA TGATTTCAGG CATGCTCCGC 
AAGAAAGTAG AAGAACTAGA CTGGGCCTGC TCAGATTTGC TGGCTCATCA GAAGCACTTG 
ACGGTCGGTT TACCACCAGA GCCCAGAGAG AAAACCATTA CAGCGCCTTT GCCTCCTGAC 
CAACTGGCCT TACTGATTGA TCAAGCCAGC GAAAACAGCA TCACTATTGC CATCCTCACA 
CAGGAGATCA TAGTGTACCT AGCGATGATC ATCAGAACGC AGCGCAGTTT GTTCAGTGAG 
ATGTTTCGGC TGAGGATCGG CCTCATCATT CAGGTTATGG CAACTGAACT GGCCCAATCT 
CTAAACTGCT CAGGTGAGGA GGCCACAGAG AGTCTGATGA AACTGAGTCC CTCTGAGTTG 
AAGAATCTTC TTCACCACAT TCTGAGCGGA AAAGAATTCG GTGTTCAGAG AAGTGTTCGT 
TCAGTTGAGC CCGGTGTCAG TCCTGTCATC TCAATCCATG AGGTGGGCAA TGTTGGAGCC 
ACCAAGAGTG AGCGCGCAGG CATCAGCAAA CTGAAGAGTG ACATGAAGAT GCTGGAAGAG 
CTCAGATTTT CATGGTCGAT CCAAAGTAAG ACTTTCTCAG GTCAGTCCTT CGATGCTGAG 
CCTGTTGAAT TAGCGAGGAG CAGGATGCCT TCAATTGATG GGTTTGACAG CTCCGTGAGT 
GTTGGTGTAC CCCGGGATAG TCGGCAGGGC CAGTGGCTGC GCAGGAGGCG GCTTGATGGA 
GCTCTCAACA GAGTTCCTGT TGGCTTTTAT CAAAAAGTCT GGAAGATTCT GCAGAAGTGT 
CATGGCCTTT CAATAGAAGG CTTCGTTCTC CCATCTTCTA CTACAAGAGA GATGACCCCA 
GGTGAGATTA AATTTGCGGT ACATGTGGAA AGGGTGCTGA ACCGTGTCCC TCAGCCTGAA 
TACAGGCAGC TGCTGGTGGA AGGCATCCTC GTCTTGACAA TGCTGGCTGA TGTTGACATT 
CAGAGCATCG GCAGCATCAT CCACATAGAA AAAATAGTTC ATATTGCTAA TGACATGTTC 
TACAAAGACC AGAAGGATCT TGGTGCTGAG GACAGTATTT TGGAGAAGGA TCAGTCTACG 
GGCATCTGTC GGCTCCTGTA TGACACTGCA CCCAGCGGCC GCTTCGGCAC CATGACTTAC 
CTTACTAAAG CTGTGGCTAC TTATGCCCAG GACTTCTTGC CTAGTGGCGC CTGTACTGTA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1a ( XM_005166519.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166576.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.

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CloneID	ODa13437
Clone ID Related Accession (Same CDS sequence)	XM_005166519.4 , XM_005166519.3
Accession Version	XM_005166519.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166519.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGCTGGAA GAGCTCAGAT TTTCATGGTC GATCCAAAGT 
AAGACTTTCT CAGGTCAGTC CTTCGATGCT GAGCCTGTTG AATTAGCGAG GAGCAGGATG 
CCTTCAATTG ATGGGTTTGA CAGCTCCGTG AGTGTTGGTG TACCCCGGGA TAGTCGGCAG 
GGCCAGTGGC TGCGCAGGAG GCGGCTTGAT GGAGCTCTCA ACAGAGTTCC TGTTGGCTTT 
TATCAAAAAG TCTGGAAGAT TCTGCAGAAG TGTCATGGCC TTTCAATAGA AGGCTTCGTT 
CTCCCATCTT CTACTACAAG AGAGATGACC CCAGGTGAGA TTAAATTTGC GGTACATGTG 
GAAAGGGTGC TGAACCGTGT CCCTCAGCCT GAATACAGGC AGCTGCTGGT GGAAGGCATC 
CTCGTCTTGA CAATGCTGGC TGATGTTGAC ATTCAGAGCA TCGGCAGCAT CATCCACATA 
GAAAAAATAG TTCATATTGC TAATGACATG TTCTACAAAG ACCAGAAGGA TCTTGGTGCT 
GAGGACAGTA TTTTGGAGAA GGATCAGTCT ACGGGCATCT GTCGGCTCCT GTATGACACT 
GCACCCAGCG GCCGCTTCGG CACCATGACT TACCTTACTA AAGCTGTGGC TACTTATGCC 
CAGGACTTCT TGCCTAGTGG CGCCTGTACT GTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166576.1
CDS	195..3893
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSGKTRSNKGWDEDDDVEDDYFRGAVHDLNFSDDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMLEELRFSWSIQSKTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_005166519.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166519.4

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGCTGGAA GAGCTCAGAT TTTCATGGTC GATCCAAAGT 
AAGACTTTCT CAGGTCAGTC CTTCGATGCT GAGCCTGTTG AATTAGCGAG GAGCAGGATG 
CCTTCAATTG ATGGGTTTGA CAGCTCCGTG AGTGTTGGTG TACCCCGGGA TAGTCGGCAG 
GGCCAGTGGC TGCGCAGGAG GCGGCTTGAT GGAGCTCTCA ACAGAGTTCC TGTTGGCTTT 
TATCAAAAAG TCTGGAAGAT TCTGCAGAAG TGTCATGGCC TTTCAATAGA AGGCTTCGTT 
CTCCCATCTT CTACTACAAG AGAGATGACC CCAGGTGAGA TTAAATTTGC GGTACATGTG 
GAAAGGGTGC TGAACCGTGT CCCTCAGCCT GAATACAGGC AGCTGCTGGT GGAAGGCATC 
CTCGTCTTGA CAATGCTGGC TGATGTTGAC ATTCAGAGCA TCGGCAGCAT CATCCACATA 
GAAAAAATAG TTCATATTGC TAATGACATG TTCTACAAAG ACCAGAAGGA TCTTGGTGCT 
GAGGACAGTA TTTTGGAGAA GGATCAGTCT ACGGGCATCT GTCGGCTCCT GTATGACACT 
GCACCCAGCG GCCGCTTCGG CACCATGACT TACCTTACTA AAGCTGTGGC TACTTATGCC 
CAGGACTTCT TGCCTAGTGG CGCCTGTACT GTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1a ( XM_005166519.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166576.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.

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CloneID	ODa13437
Clone ID Related Accession (Same CDS sequence)	XM_005166519.4 , XM_005166519.3
Accession Version	XM_005166519.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGCTGGAA GAGCTCAGAT TTTCATGGTC GATCCAAAGT 
AAGACTTTCT CAGGTCAGTC CTTCGATGCT GAGCCTGTTG AATTAGCGAG GAGCAGGATG 
CCTTCAATTG ATGGGTTTGA CAGCTCCGTG AGTGTTGGTG TACCCCGGGA TAGTCGGCAG 
GGCCAGTGGC TGCGCAGGAG GCGGCTTGAT GGAGCTCTCA ACAGAGTTCC TGTTGGCTTT 
TATCAAAAAG TCTGGAAGAT TCTGCAGAAG TGTCATGGCC TTTCAATAGA AGGCTTCGTT 
CTCCCATCTT CTACTACAAG AGAGATGACC CCAGGTGAGA TTAAATTTGC GGTACATGTG 
GAAAGGGTGC TGAACCGTGT CCCTCAGCCT GAATACAGGC AGCTGCTGGT GGAAGGCATC 
CTCGTCTTGA CAATGCTGGC TGATGTTGAC ATTCAGAGCA TCGGCAGCAT CATCCACATA 
GAAAAAATAG TTCATATTGC TAATGACATG TTCTACAAAG ACCAGAAGGA TCTTGGTGCT 
GAGGACAGTA TTTTGGAGAA GGATCAGTCT ACGGGCATCT GTCGGCTCCT GTATGACACT 
GCACCCAGCG GCCGCTTCGG CACCATGACT TACCTTACTA AAGCTGTGGC TACTTATGCC 
CAGGACTTCT TGCCTAGTGG CGCCTGTACT GTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166576.1
CDS	177..3875
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSGKTRSNKGWDEDDDVEDDYFRGAVHDLNFSDDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMLEELRFSWSIQSKTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_005166519.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166519.3

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGCTGGAA GAGCTCAGAT TTTCATGGTC GATCCAAAGT 
AAGACTTTCT CAGGTCAGTC CTTCGATGCT GAGCCTGTTG AATTAGCGAG GAGCAGGATG 
CCTTCAATTG ATGGGTTTGA CAGCTCCGTG AGTGTTGGTG TACCCCGGGA TAGTCGGCAG 
GGCCAGTGGC TGCGCAGGAG GCGGCTTGAT GGAGCTCTCA ACAGAGTTCC TGTTGGCTTT 
TATCAAAAAG TCTGGAAGAT TCTGCAGAAG TGTCATGGCC TTTCAATAGA AGGCTTCGTT 
CTCCCATCTT CTACTACAAG AGAGATGACC CCAGGTGAGA TTAAATTTGC GGTACATGTG 
GAAAGGGTGC TGAACCGTGT CCCTCAGCCT GAATACAGGC AGCTGCTGGT GGAAGGCATC 
CTCGTCTTGA CAATGCTGGC TGATGTTGAC ATTCAGAGCA TCGGCAGCAT CATCCACATA 
GAAAAAATAG TTCATATTGC TAATGACATG TTCTACAAAG ACCAGAAGGA TCTTGGTGCT 
GAGGACAGTA TTTTGGAGAA GGATCAGTCT ACGGGCATCT GTCGGCTCCT GTATGACACT 
GCACCCAGCG GCCGCTTCGG CACCATGACT TACCTTACTA AAGCTGTGGC TACTTATGCC 
CAGGACTTCT TGCCTAGTGG CGCCTGTACT GTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1a ( XM_009303454.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301729.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13438
Clone ID Related Accession (Same CDS sequence)	XM_009303454.3 , XM_009303454.2
Accession Version	XM_009303454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGACTTTC TCAGGTCAGT CCTTCGATGC TGAGCCTGTT 
GAATTAGCGA GGAGCAGGAT GCCTTCAATT GATGGGTTTG ACAGCTCCGT GAGTGTTGGT 
GTACCCCGGG ATAGTCGGCA GGGCCAGTGG CTGCGCAGGA GGCGGCTTGA TGGAGCTCTC 
AACAGAGTTC CTGTTGGCTT TTATCAAAAA GTCTGGAAGA TTCTGCAGAA GTGTCATGGC 
CTTTCAATAG AAGGCTTCGT TCTCCCATCT TCTACTACAA GAGAGATGAC CCCAGGTGAG 
ATTAAATTTG CGGTACATGT GGAAAGGGTG CTGAACCGTG TCCCTCAGCC TGAATACAGG 
CAGCTGCTGG TGGAAGGCAT CCTCGTCTTG ACAATGCTGG CTGATGTTGA CATTCAGAGC 
ATCGGCAGCA TCATCCACAT AGAAAAAATA GTTCATATTG CTAATGACAT GTTCTACAAA 
GACCAGAAGG ATCTTGGTGC TGAGGACAGT ATTTTGGAGA AGGATCAGTC TACGGGCATC 
TGTCGGCTCC TGTATGACAC TGCACCCAGC GGCCGCTTCG GCACCATGAC TTACCTTACT 
AAAGCTGTGG CTACTTATGC CCAGGACTTC TTGCCTAGTG GCGCCTGTAC TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301729.1
CDS	195..3854
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSGKTRSNKGWDEDDDVEDDYFRGAVHDLNFSDDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_009303454.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303454.3

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGACTTTC TCAGGTCAGT CCTTCGATGC TGAGCCTGTT 
GAATTAGCGA GGAGCAGGAT GCCTTCAATT GATGGGTTTG ACAGCTCCGT GAGTGTTGGT 
GTACCCCGGG ATAGTCGGCA GGGCCAGTGG CTGCGCAGGA GGCGGCTTGA TGGAGCTCTC 
AACAGAGTTC CTGTTGGCTT TTATCAAAAA GTCTGGAAGA TTCTGCAGAA GTGTCATGGC 
CTTTCAATAG AAGGCTTCGT TCTCCCATCT TCTACTACAA GAGAGATGAC CCCAGGTGAG 
ATTAAATTTG CGGTACATGT GGAAAGGGTG CTGAACCGTG TCCCTCAGCC TGAATACAGG 
CAGCTGCTGG TGGAAGGCAT CCTCGTCTTG ACAATGCTGG CTGATGTTGA CATTCAGAGC 
ATCGGCAGCA TCATCCACAT AGAAAAAATA GTTCATATTG CTAATGACAT GTTCTACAAA 
GACCAGAAGG ATCTTGGTGC TGAGGACAGT ATTTTGGAGA AGGATCAGTC TACGGGCATC 
TGTCGGCTCC TGTATGACAC TGCACCCAGC GGCCGCTTCG GCACCATGAC TTACCTTACT 
AAAGCTGTGG CTACTTATGC CCAGGACTTC TTGCCTAGTG GCGCCTGTAC TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1a ( XM_009303454.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301729.1 Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.

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CloneID	ODa13438
Clone ID Related Accession (Same CDS sequence)	XM_009303454.3 , XM_009303454.2
Accession Version	XM_009303454.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGACTTTC TCAGGTCAGT CCTTCGATGC TGAGCCTGTT 
GAATTAGCGA GGAGCAGGAT GCCTTCAATT GATGGGTTTG ACAGCTCCGT GAGTGTTGGT 
GTACCCCGGG ATAGTCGGCA GGGCCAGTGG CTGCGCAGGA GGCGGCTTGA TGGAGCTCTC 
AACAGAGTTC CTGTTGGCTT TTATCAAAAA GTCTGGAAGA TTCTGCAGAA GTGTCATGGC 
CTTTCAATAG AAGGCTTCGT TCTCCCATCT TCTACTACAA GAGAGATGAC CCCAGGTGAG 
ATTAAATTTG CGGTACATGT GGAAAGGGTG CTGAACCGTG TCCCTCAGCC TGAATACAGG 
CAGCTGCTGG TGGAAGGCAT CCTCGTCTTG ACAATGCTGG CTGATGTTGA CATTCAGAGC 
ATCGGCAGCA TCATCCACAT AGAAAAAATA GTTCATATTG CTAATGACAT GTTCTACAAA 
GACCAGAAGG ATCTTGGTGC TGAGGACAGT ATTTTGGAGA AGGATCAGTC TACGGGCATC 
TGTCGGCTCC TGTATGACAC TGCACCCAGC GGCCGCTTCG GCACCATGAC TTACCTTACT 
AAAGCTGTGG CTACTTATGC CCAGGACTTC TTGCCTAGTG GCGCCTGTAC TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301729.1
CDS	177..3836
Translation	MRSRSNSGVKLDNYARIVHQTILRHQDPVTGLLPASKEQPDAWVRDNVYSILSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSKSALDSLHAKYNTRTCATVVGDREWGHLQLDATSLYMLFLAQMTASGLHIVYTQDEVDVVQNLLFYIEAAYKVADYGMWERGDKTNQGIPELNASSIGMAKAALEALDDLNLFGAKGGPDSVVHVLADDIQHCQSILNSMLPRASTSKEVDAGLLSIISYPAFAVEDMELVNITKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFINSPEQVEEYREALEGILIKQKDGIRLLPELYSVPPEKVDEEYVNPHTVERIPLGKCPLKWGQSLYVLGNLLAEGFLAPGEIDPLNRRFSAIPKPDVVVQVSILAENEDIKALLQKNGIDVETVADIHPIRVQPSRVLSHIYARLGRNERLGLTGRPYRRIGVLGTSKLYIIRNTIFSFTPQFIDHQQFYLALDNKMIVEMLRTDLSYLSSRWRMTGRPTVTFPISQTMLNHDHTDLDPAVLATLKKLQDGYFGGARVQTGKLSEFLTTCCCAHLSFLDSGKTRSNKGWDEDDDVEDDYFRGAVHDLNFSDDSDDLAQYLDQLLHAAVPVQGLRVEAEAKGLSRFKAAATKTREMVSLVNKAKDLQIHNLHMYLPNKLFRSPQPALNLKDFPGLGTDHSSEVVVPSENNLPRDTHGAIDYSSLVQVLKDTQSLHDQADILYILFKDKGMDWDTQLHGKGTTVKSLLVELYEKAGDLRCWDIIRMISGMLRKKVEELDWACSDLLAHQKHLTVGLPPEPREKTITAPLPPDQLALLIDQASENSITIAILTQEIIVYLAMIIRTQRSLFSEMFRLRIGLIIQVMATELAQSLNCSGEEATESLMKLSPSELKNLLHHILSGKEFGVQRSVRSVEPGVSPVISIHEVGNVGATKSERAGISKLKSDMKMTFSGQSFDAEPVELARSRMPSIDGFDSSVSVGVPRDSRQGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFAVHVERVLNRVPQPEYRQLLVEGILVLTMLADVDIQSIGSIIHIEKIVHIANDMFYKDQKDLGAEDSILEKDQSTGICRLLYDTAPSGRFGTMTYLTKAVATYAQDFLPSGACTVQ

Target ORF information:

RefSeq Version	XM_009303454.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 1a (muscle) (phka1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303454.2

ORF Insert Sequence:

ATGAGGAGTC GCAGCAACTC TGGAGTTAAA CTTGACAACT ACGCGCGGAT AGTTCATCAG 
ACTATTCTGC GTCATCAAGA TCCAGTGACT GGTCTGTTGC CAGCAAGCAA GGAGCAGCCT 
GATGCCTGGG TGAGAGACAA TGTTTACAGC ATCCTGTCGG TCTGGGCTCT TAGTCTGGCT 
TACAGGAAAA ATGCAGATCG TGATGAAGAC AAGGCTAAAG CCTACGAGCT CGAGCAGAGT 
GTGGTGAAGT TAATGAGGGG AGTTCTTCAA TGCATTATGA GACAGCTGGA CAAAGTGGAG 
AAATTCAAAT ACAGTAAAAG CGCTTTGGAC AGCCTCCACG CCAAGTATAA CACTCGAACC 
TGTGCCACTG TTGTGGGTGA CCGGGAATGG GGGCATCTCC AGCTCGACGC CACCTCCCTA 
TACATGCTCT TCCTAGCCCA GATGACTGCC TCAGGGCTGC ACATAGTGTA CACTCAAGAC 
GAGGTGGATG TGGTCCAGAA TCTGCTCTTC TACATTGAAG CTGCATACAA AGTGGCTGAC 
TACGGGATGT GGGAGAGAGG AGACAAAACA AATCAGGGCA TCCCTGAGCT CAATGCCAGC 
TCAATTGGGA TGGCTAAAGC AGCCCTTGAA GCCCTAGATG ACTTGAATTT GTTTGGGGCC 
AAAGGGGGAC CGGACTCTGT GGTTCATGTC TTAGCAGATG ATATCCAGCA CTGTCAGTCT 
ATTCTGAACT CCATGTTGCC CAGAGCTTCA ACATCCAAAG AGGTGGATGC CGGGCTTCTG 
TCTATTATTT CATATCCAGC CTTTGCAGTG GAAGACATGG AGCTAGTCAA TATCACCAAA 
GAGGAAATCA TCTCCAAGCT CCAGGGAAGA TACGGCTGTT GTCGGTTTTT AAGAGATGGC 
CACAAAACTC CTAAAGAGGA CCCAAACAGG TTGTATTATG AATCTGCCGA GCTGAAGCTT 
TTTGAAAACA TTGAATGCGA GTGGCCACTT TTCTGGACCT ACCTGATCTT GGATGGCATC 
TTTATCAACA GTCCAGAGCA GGTTGAGGAG TACAGAGAAG CTCTGGAAGG TATCCTAATC 
AAACAGAAAG ATGGGATTCG GTTGCTGCCT GAGCTCTACA GTGTTCCTCC TGAGAAGGTG 
GATGAGGAGT ATGTTAATCC TCACACGGTG GAGAGGATCC CGTTGGGAAA GTGCCCTCTG 
AAGTGGGGCC AGTCCCTTTA CGTTCTGGGC AACCTGTTGG CAGAGGGTTT TTTGGCTCCT 
GGAGAGATCG ACCCCCTAAA CCGCAGGTTC TCTGCCATCC CTAAACCAGA TGTTGTTGTG 
CAAGTGTCCA TCCTGGCTGA GAACGAGGAC ATTAAGGCGC TCCTGCAGAA GAATGGGATT 
GATGTGGAGA CAGTGGCAGA CATCCACCCA ATAAGAGTGC AGCCGTCTCG AGTTCTCAGC 
CACATTTATG CTCGGCTAGG GCGCAATGAG AGGCTGGGGC TGACCGGCAG ACCATACCGA 
AGGATCGGTG TGTTGGGAAC CTCAAAGCTG TACATCATCC GGAACACCAT CTTCAGCTTC 
ACACCTCAGT TCATAGATCA TCAGCAGTTC TATTTGGCCC TGGATAACAA GATGATTGTG 
GAGATGTTGC GCACTGACCT GTCTTACCTG TCTTCTCGTT GGAGGATGAC CGGCCGTCCG 
ACTGTCACTT TTCCCATCTC CCAGACTATG CTTAACCATG ACCACACTGA CTTGGACCCT 
GCTGTTCTGG CCACACTTAA GAAGCTTCAG GATGGGTACT TCGGAGGAGC AAGGGTGCAG 
ACTGGGAAGT TATCAGAGTT TCTGACTACG TGTTGCTGTG CTCACCTGAG CTTTCTGGAC 
AGTGGTAAGA CACGCTCCAA TAAGGGCTGG GATGAAGATG ATGATGTTGA GGATGACTAC 
TTTAGAGGTG CTGTGCATGA CCTGAACTTC AGTGATGATT CAGATGATCT AGCACAATAC 
CTGGACCAGC TTTTGCATGC AGCGGTGCCT GTCCAGGGGC TCAGAGTCGA GGCAGAGGCT 
AAAGGACTGA GTCGCTTTAA GGCTGCAGCC ACAAAGACAC GTGAGATGGT GTCTCTTGTG 
AACAAAGCCA AGGATCTACA AATCCACAAT CTCCATATGT ATCTTCCCAA TAAGCTGTTC 
CGCTCACCTC AGCCTGCGCT TAATTTAAAA GACTTTCCAG GACTGGGGAC GGATCATAGT 
TCAGAAGTTG TGGTGCCCTC TGAAAATAAC CTGCCTCGGG ACACTCATGG TGCCATTGAC 
TACAGTAGTC TTGTGCAGGT CCTTAAAGAC ACTCAGAGCC TTCACGATCA GGCAGACATC 
CTCTACATTC TTTTTAAAGA CAAGGGAATG GACTGGGACA CTCAGCTGCA TGGGAAAGGC 
ACCACTGTTA AGAGTTTGCT AGTCGAGCTC TATGAGAAAG CGGGAGATCT GAGGTGTTGG 
GATATCATCC GTATGATTTC AGGCATGCTC CGCAAGAAAG TAGAAGAACT AGACTGGGCC 
TGCTCAGATT TGCTGGCTCA TCAGAAGCAC TTGACGGTCG GTTTACCACC AGAGCCCAGA 
GAGAAAACCA TTACAGCGCC TTTGCCTCCT GACCAACTGG CCTTACTGAT TGATCAAGCC 
AGCGAAAACA GCATCACTAT TGCCATCCTC ACACAGGAGA TCATAGTGTA CCTAGCGATG 
ATCATCAGAA CGCAGCGCAG TTTGTTCAGT GAGATGTTTC GGCTGAGGAT CGGCCTCATC 
ATTCAGGTTA TGGCAACTGA ACTGGCCCAA TCTCTAAACT GCTCAGGTGA GGAGGCCACA 
GAGAGTCTGA TGAAACTGAG TCCCTCTGAG TTGAAGAATC TTCTTCACCA CATTCTGAGC 
GGAAAAGAAT TCGGTGTTCA GAGAAGTGTT CGTTCAGTTG AGCCCGGTGT CAGTCCTGTC 
ATCTCAATCC ATGAGGTGGG CAATGTTGGA GCCACCAAGA GTGAGCGCGC AGGCATCAGC 
AAACTGAAGA GTGACATGAA GATGACTTTC TCAGGTCAGT CCTTCGATGC TGAGCCTGTT 
GAATTAGCGA GGAGCAGGAT GCCTTCAATT GATGGGTTTG ACAGCTCCGT GAGTGTTGGT 
GTACCCCGGG ATAGTCGGCA GGGCCAGTGG CTGCGCAGGA GGCGGCTTGA TGGAGCTCTC 
AACAGAGTTC CTGTTGGCTT TTATCAAAAA GTCTGGAAGA TTCTGCAGAA GTGTCATGGC 
CTTTCAATAG AAGGCTTCGT TCTCCCATCT TCTACTACAA GAGAGATGAC CCCAGGTGAG 
ATTAAATTTG CGGTACATGT GGAAAGGGTG CTGAACCGTG TCCCTCAGCC TGAATACAGG 
CAGCTGCTGG TGGAAGGCAT CCTCGTCTTG ACAATGCTGG CTGATGTTGA CATTCAGAGC 
ATCGGCAGCA TCATCCACAT AGAAAAAATA GTTCATATTG CTAATGACAT GTTCTACAAA 
GACCAGAAGG ATCTTGGTGC TGAGGACAGT ATTTTGGAGA AGGATCAGTC TACGGGCATC 
TGTCGGCTCC TGTATGACAC TGCACCCAGC GGCCGCTTCG GCACCATGAC TTACCTTACT 
AAAGCTGTGG CTACTTATGC CCAGGACTTC TTGCCTAGTG GCGCCTGTAC TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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phka1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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