phka1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka1
Entrez Gene ID	100158624
Full Name	phosphorylase kinase, alpha 1 (muscle)
General protein information	Preferred Names phosphorylase kinase, alpha 1 (muscle) Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096167.1	XP_017951656.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_031890231.1	XP_031746091.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
NM_001128038.1	NP_001121510.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
XM_031890232.1	XP_031746092.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_031890233.1	XP_031746093.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following phka1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45980	XM_018096167.1 Latest version!	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa63516	XM_031890231.1 Latest version!	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63516	XM_031890232.1 Latest version!	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63517	XM_031890233.1 Latest version!	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa20534	NM_001128038.1 Latest version!	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka1 ( XM_018096167.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951656.1 Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45980
Clone ID Related Accession (Same CDS sequence)	XM_018096167.1
Accession Version	XM_018096167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGCTTCTAC AGTTTTTTGG TCTCAGGGTG GAGGACTTAG TCAGGGGATG GCACTGGAGA 
GTCCCACAGA GGGGACAGCA GTTTAGTGAG GAGGTGGTAA GAGAGACCAC GCTCGCCTAC 
AGTGAAAGTA TACCTCAGGA GGTGCTAGCT CCGCCATCCT ACCGACAAGC CTTAAAGGAT 
GGGGCTAGGC CTTTGGTGAG GGCAAGTGGG TCCGCCAGCA GCAGTACTCC CGGCTGGGAG 
GAGGACCTGG GGGAAATGGA GGAGGACGGT GCCTTTAGCC TCCCCTTGTT CAAGCTGCCT 
GATAGCTTCT GGGCCCCACA CAAAATCACC AAAGAGGAGG AAAAGGACTT TTGGGTCTAC 
ACGGCCGGGC AGACCCAGAC ATATTCTGCA GGGTGTCCCG CATCCAGCGG GTCATTAACT 
TTAAGATTTT CAGGCTCTGG GGGTACTATA TGCCCTACGC ACCCCTGTGG GTGTATGACA 
AGATGGAGGG CCTGGTGGAT CACTGCCAAA GAGAAAGGGG TGGGAAAGTC CCTTACTCAC 
CCAGAACAGG CCAGACAGAT GGCCGCATGG AGGTTTGTGT GGGCCATAGA GAAGGAGTGG 
TGGTGGGGGC ACACCGTGCT GGGCCACACT GTCCACAGCT GTGGGAGAAA GAACCCAGAG 
CCCCGCTGCT TCAGTACAGA AGTCACCTTA CCTAATGGAG CCAAGGTGGA GAAGCCCCAC 
CCTAAATACT ACGCTTCGTA TAAGGATGTG AAGGAAAGCC ATTGTACCCT TGACAGTCCT 
CAGGAGAGGC TGTTGAAGTG TGATGTTAAA AGTTTTGGGG CCACATTTCA GCCAGTTATG 
AGGAGCAGGA GTAACTCTGG TGTTCGCCTG GATAGATATG CCAAGCTTGT CCAACAGACC 
ATCCTCTGCC ACCAGAATCC AGTCACGGGT CTGTTGCCAG CCAGCTCTGA TCAGAAGGAT 
GCCTGGGTAC GTGACAACGT GTACACAATT CTGGCCGTGT GGGGGCTTGG TCTCGCATAT 
CGGAAAAATG CTGATCGGGA TGAAGATAAA GCAAAGGCAT ATGAGCTGGA ACAGAATGTG 
GTCAAGCTGA TGAGAGGCCT GCTTCAATGT ATGATGAGAC AGGTTTCCAA AGTGGAAGAG 
TTTAAATATA GCCAGAATAC CAAGGATGCC CTACATGCTA AATATAATAC CAACACCTGC 
TCCACTGTGG TGGGCGATGA CCAGTGGGGC CACCTACAGC TTGATGCCAC ATCCATATAC 
CTGCTGTTCC TGGCACAAAT GACTGCATCA GGCCTGCACA TAATCTACAA TCTGGATGAA 
GTGAATTTTA TACAGAACCT GGTCTTTTAT GTTGAAGCAG CATACAAAAC AGCGGATTTT 
GGAATGTGGG AACGAGGTGA CAAAACCAAC CAGGGCATTA CTGAGTTGAA TGCTAGCTCT 
GTGGGCATGG CCAAGGCTGC CCTTGAGTCT TTAGATGAGC TGAACTTGTT TGGTGCTAAG 
GGAGGTCCCC AGTCTGTCAT ACATGTCCTG TCTGATGAAA TCCAGCACTG CCAGTCTATC 
CTACACTCCA TGCTTCCAAG AGCTTCCACC TCTAAGGAGA TAGATGCAGG GGTTCTGTCT 
GTTATCTCAT TTCCAGCCTT TGCTGTGGAG GACAGTCATC TAGTGCGGAT TACTAAACAA 
GAGATCATTG ACAAGTTACA GGATCCCGGC AGACTATATT ATGAGCCTGC AGAACTCAAA 
CTCTTTGAAA ATATTGAATG TGAATGGCCG TTATTTTGGA CCTATTTTAT TCTGGATGGG 
ATATTCAGTA GTAACCATGA GCAGGTTCAA GAATACAAGC AAGCCATTGA TGAGGTCTTA 
ATAAAAGGAA AGTCCGGAGT GAGACTGGTC CCAGAGCTGT ACAGTGTCCC AGAGGACAAA 
GTGGAGGTGG AATATGAGCA CCCACACACA GTAGATCGCA TACCCTTGGG GAAGCTGCCA 
CACATGTGGG GACAATCTTT ATATATACTG GGATGTCTAA TGTCTGAGGG CTTCCTGGCT 
CCTGGGGAGA TTGATCCCCT GAACAGGAGA TTCTCTACAG TCCCTAAGCC TGATGTGGTT 
GTCCAAGTTT CTATCCTAGC AGAAACCGAT GAGATTCAAG AAATATTGAA GAAGCATGGA 
ATTGAAGCAG AGACAGTTGC TGGAGTGTAC CCAATTAGAG TGCAGCCGGC CAGGATTCTG 
AGTCACATCT ATGCACGGCT TGGTCGTAAC CGGAAAATGA ATCTCAGTGG AAGACCCTAC 
AGAAATATGG GCGTTCTGGG TACCTCAAAG CTCTACACCA TTCGCCAGAA CATCTTTGCC 
TTCACCCCAC AGTTCATAGA TCAGCAGCAG TTCTATCTGG CTTTGGATAA CCGGATGATT 
GTGGAGATGT TGCGCACTGA CCTATCTTAC CTGTGCAGTC GCTGGAGAAT GACCGGCCGC 
CCAACCATCA CCTTCCCAAT CTCTCACACT ATGCTAGATG ATTCTGGTAA TGACCTTGAC 
CCAGTTGTCC TTGCCACAAT CAGAAAACTA CAGGATGGAT ATTTTGGGGG TGCCAGGACC 
CAGACCGGCT TCCTGTCGAA ATTCCTAAAC ACATCCTGCT GCAAACACCT GAGCTTCATG 
GATGCCGGTG TGGAGGGTAA GCTGAGCACC TGGGGCAGTG TCAACCGTGG GGGCCAGCCA 
ATCATCGTGC GGGAGCCAGA TGAGGCCCCA TCAGCCGATG ATGAGGATGT GGATGACGAA 
GTGTCACTGT ATCTTAATCA ACTACTTGGC ATGAGCTCCC CCAAACCAAG TCCTACATCA 
CAGCGAGGGG GTCGGCATCG CTTTAAAGCT GCCATTCACA GTACACGAGA TATGTTATCT 
CTGCGGAGCA AAGCCAAGGG TCTTAATGTG CAAGACATAC AGATGTACCT CCCATCAAAA 
CTGTTCCTGG CATCACAGCC CTCCTTTGAT TTGCTGGAAT CGGCTCCGAG CTACCAGGCT 
TCAGGAAGGA TCCCAACAAT CCATGCAGAA CTTCATCTTC CTAAGGACAC AGAGGGGCAG 
GTAGATTATG CAGCTATGGT CGATCATTTA CGGGAAACCA ACAATTTACA GGAACAAGCG 
GACATCTTGT ACCTGCTGTA CACCATGAAA GGAGGAGATT GGGACACAAA AATATATGGA 
CAGCCAGGTG TGACCGTTAA GGATCTCCTG AAGGATCTGT ATGGTAAAGT GGGTTCCATG 
CGGCAATGGG CACTGATCCG AGGCATTTCT GGGATACTGA AAAAGAAAGT GGAGGCACTG 
GATGAGGCCT GCACAGACCT TCTGTCCCAT CAGAAGCAAC TAACCGTAGG CCTGCCTCCT 
GAGCCACGGG AAATAGCCAT CACAGCGCCT CTACCTTATG AAAAACTCAT TCGTCTTATT 
GATGAAGCCA GTGAGCAAAA TAGGACCATA TCCATCCTGA CTCAGGAAAT TATGGTGTAT 
CTGGCAATGT ACATGAGGAC CCAGCCATCT CTGTTTGCTG AGATGTTCCG ACTGCGCATT 
GGGCTCATCA TACAGGTCAT GGCTACAGAA CTGTCCCATT CACTCCGTTG CTCTGCTGAG 
GAAGCCACGG AAAGCCTTAT GAACCTCAGC CCGTCTGACA TGAAGAACCT TCTTCACCAT 
ATTCTGAGCG GAAAGGAATT TGGGGTAGAG AGAAGTGTAC GAGGTGGAGA ACCTTGGGTC 
AGTCCAGCGA TTTCTATTCA TGAACTTGGA GCTGTGGGAG CCACAAAGAG TGAAAGAGCA 
GGTATTACCC GACTAAAGAG TGAGATCAAG CTGCTGGATA AACGTAGGCT GTCTCTGGCT 
GCAGAAGGCC AGGTGAAATA TTCCTTCACT GTACATAAAT CACTGCTGAG TTTACACGCG 
GCTGCTGAAA CACCGCATGG GCTCTTCACT TGGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951656.1
CDS	1..3939
Translation	MLLQFFGLRVEDLVRGWHWRVPQRGQQFSEEVVRETTLAYSESIPQEVLAPPSYRQALKDGARPLVRASGSASSSTPGWEEDLGEMEEDGAFSLPLFKLPDSFWAPHKITKEEEKDFWVYTAGQTQTYSAGCPASSGSLTLRFSGSGGTICPTHPCGCMTRWRAWWITAKEKGVGKSLTHPEQARQMAAWRFVWAIEKEWWWGHTVLGHTVHSCGRKNPEPRCFSTEVTLPNGAKVEKPHPKYYASYKDVKESHCTLDSPQERLLKCDVKSFGATFQPVMRSRSNSGVRLDRYAKLVQQTILCHQNPVTGLLPASSDQKDAWVRDNVYTILAVWGLGLAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVSKVEEFKYSQNTKDALHAKYNTNTCSTVVGDDQWGHLQLDATSIYLLFLAQMTASGLHIIYNLDEVNFIQNLVFYVEAAYKTADFGMWERGDKTNQGITELNASSVGMAKAALESLDELNLFGAKGGPQSVIHVLSDEIQHCQSILHSMLPRASTSKEIDAGVLSVISFPAFAVEDSHLVRITKQEIIDKLQDPGRLYYEPAELKLFENIECEWPLFWTYFILDGIFSSNHEQVQEYKQAIDEVLIKGKSGVRLVPELYSVPEDKVEVEYEHPHTVDRIPLGKLPHMWGQSLYILGCLMSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIQEILKKHGIEAETVAGVYPIRVQPARILSHIYARLGRNRKMNLSGRPYRNMGVLGTSKLYTIRQNIFAFTPQFIDQQQFYLALDNRMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDDSGNDLDPVVLATIRKLQDGYFGGARTQTGFLSKFLNTSCCKHLSFMDAGVEGKLSTWGSVNRGGQPIIVREPDEAPSADDEDVDDEVSLYLNQLLGMSSPKPSPTSQRGGRHRFKAAIHSTRDMLSLRSKAKGLNVQDIQMYLPSKLFLASQPSFDLLESAPSYQASGRIPTIHAELHLPKDTEGQVDYAAMVDHLRETNNLQEQADILYLLYTMKGGDWDTKIYGQPGVTVKDLLKDLYGKVGSMRQWALIRGISGILKKKVEALDEACTDLLSHQKQLTVGLPPEPREIAITAPLPYEKLIRLIDEASEQNRTISILTQEIMVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELSHSLRCSAEEATESLMNLSPSDMKNLLHHILSGKEFGVERSVRGGEPWVSPAISIHELGAVGATKSERAGITRLKSEIKLLDKRRLSLAAEGQVKYSFTVHKSLLSLHAAAETPHGLFTWG

Target ORF information:

RefSeq Version	XM_018096167.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096167.1

ORF Insert Sequence:

ATGCTTCTAC AGTTTTTTGG TCTCAGGGTG GAGGACTTAG TCAGGGGATG GCACTGGAGA 
GTCCCACAGA GGGGACAGCA GTTTAGTGAG GAGGTGGTAA GAGAGACCAC GCTCGCCTAC 
AGTGAAAGTA TACCTCAGGA GGTGCTAGCT CCGCCATCCT ACCGACAAGC CTTAAAGGAT 
GGGGCTAGGC CTTTGGTGAG GGCAAGTGGG TCCGCCAGCA GCAGTACTCC CGGCTGGGAG 
GAGGACCTGG GGGAAATGGA GGAGGACGGT GCCTTTAGCC TCCCCTTGTT CAAGCTGCCT 
GATAGCTTCT GGGCCCCACA CAAAATCACC AAAGAGGAGG AAAAGGACTT TTGGGTCTAC 
ACGGCCGGGC AGACCCAGAC ATATTCTGCA GGGTGTCCCG CATCCAGCGG GTCATTAACT 
TTAAGATTTT CAGGCTCTGG GGGTACTATA TGCCCTACGC ACCCCTGTGG GTGTATGACA 
AGATGGAGGG CCTGGTGGAT CACTGCCAAA GAGAAAGGGG TGGGAAAGTC CCTTACTCAC 
CCAGAACAGG CCAGACAGAT GGCCGCATGG AGGTTTGTGT GGGCCATAGA GAAGGAGTGG 
TGGTGGGGGC ACACCGTGCT GGGCCACACT GTCCACAGCT GTGGGAGAAA GAACCCAGAG 
CCCCGCTGCT TCAGTACAGA AGTCACCTTA CCTAATGGAG CCAAGGTGGA GAAGCCCCAC 
CCTAAATACT ACGCTTCGTA TAAGGATGTG AAGGAAAGCC ATTGTACCCT TGACAGTCCT 
CAGGAGAGGC TGTTGAAGTG TGATGTTAAA AGTTTTGGGG CCACATTTCA GCCAGTTATG 
AGGAGCAGGA GTAACTCTGG TGTTCGCCTG GATAGATATG CCAAGCTTGT CCAACAGACC 
ATCCTCTGCC ACCAGAATCC AGTCACGGGT CTGTTGCCAG CCAGCTCTGA TCAGAAGGAT 
GCCTGGGTAC GTGACAACGT GTACACAATT CTGGCCGTGT GGGGGCTTGG TCTCGCATAT 
CGGAAAAATG CTGATCGGGA TGAAGATAAA GCAAAGGCAT ATGAGCTGGA ACAGAATGTG 
GTCAAGCTGA TGAGAGGCCT GCTTCAATGT ATGATGAGAC AGGTTTCCAA AGTGGAAGAG 
TTTAAATATA GCCAGAATAC CAAGGATGCC CTACATGCTA AATATAATAC CAACACCTGC 
TCCACTGTGG TGGGCGATGA CCAGTGGGGC CACCTACAGC TTGATGCCAC ATCCATATAC 
CTGCTGTTCC TGGCACAAAT GACTGCATCA GGCCTGCACA TAATCTACAA TCTGGATGAA 
GTGAATTTTA TACAGAACCT GGTCTTTTAT GTTGAAGCAG CATACAAAAC AGCGGATTTT 
GGAATGTGGG AACGAGGTGA CAAAACCAAC CAGGGCATTA CTGAGTTGAA TGCTAGCTCT 
GTGGGCATGG CCAAGGCTGC CCTTGAGTCT TTAGATGAGC TGAACTTGTT TGGTGCTAAG 
GGAGGTCCCC AGTCTGTCAT ACATGTCCTG TCTGATGAAA TCCAGCACTG CCAGTCTATC 
CTACACTCCA TGCTTCCAAG AGCTTCCACC TCTAAGGAGA TAGATGCAGG GGTTCTGTCT 
GTTATCTCAT TTCCAGCCTT TGCTGTGGAG GACAGTCATC TAGTGCGGAT TACTAAACAA 
GAGATCATTG ACAAGTTACA GGATCCCGGC AGACTATATT ATGAGCCTGC AGAACTCAAA 
CTCTTTGAAA ATATTGAATG TGAATGGCCG TTATTTTGGA CCTATTTTAT TCTGGATGGG 
ATATTCAGTA GTAACCATGA GCAGGTTCAA GAATACAAGC AAGCCATTGA TGAGGTCTTA 
ATAAAAGGAA AGTCCGGAGT GAGACTGGTC CCAGAGCTGT ACAGTGTCCC AGAGGACAAA 
GTGGAGGTGG AATATGAGCA CCCACACACA GTAGATCGCA TACCCTTGGG GAAGCTGCCA 
CACATGTGGG GACAATCTTT ATATATACTG GGATGTCTAA TGTCTGAGGG CTTCCTGGCT 
CCTGGGGAGA TTGATCCCCT GAACAGGAGA TTCTCTACAG TCCCTAAGCC TGATGTGGTT 
GTCCAAGTTT CTATCCTAGC AGAAACCGAT GAGATTCAAG AAATATTGAA GAAGCATGGA 
ATTGAAGCAG AGACAGTTGC TGGAGTGTAC CCAATTAGAG TGCAGCCGGC CAGGATTCTG 
AGTCACATCT ATGCACGGCT TGGTCGTAAC CGGAAAATGA ATCTCAGTGG AAGACCCTAC 
AGAAATATGG GCGTTCTGGG TACCTCAAAG CTCTACACCA TTCGCCAGAA CATCTTTGCC 
TTCACCCCAC AGTTCATAGA TCAGCAGCAG TTCTATCTGG CTTTGGATAA CCGGATGATT 
GTGGAGATGT TGCGCACTGA CCTATCTTAC CTGTGCAGTC GCTGGAGAAT GACCGGCCGC 
CCAACCATCA CCTTCCCAAT CTCTCACACT ATGCTAGATG ATTCTGGTAA TGACCTTGAC 
CCAGTTGTCC TTGCCACAAT CAGAAAACTA CAGGATGGAT ATTTTGGGGG TGCCAGGACC 
CAGACCGGCT TCCTGTCGAA ATTCCTAAAC ACATCCTGCT GCAAACACCT GAGCTTCATG 
GATGCCGGTG TGGAGGGTAA GCTGAGCACC TGGGGCAGTG TCAACCGTGG GGGCCAGCCA 
ATCATCGTGC GGGAGCCAGA TGAGGCCCCA TCAGCCGATG ATGAGGATGT GGATGACGAA 
GTGTCACTGT ATCTTAATCA ACTACTTGGC ATGAGCTCCC CCAAACCAAG TCCTACATCA 
CAGCGAGGGG GTCGGCATCG CTTTAAAGCT GCCATTCACA GTACACGAGA TATGTTATCT 
CTGCGGAGCA AAGCCAAGGG TCTTAATGTG CAAGACATAC AGATGTACCT CCCATCAAAA 
CTGTTCCTGG CATCACAGCC CTCCTTTGAT TTGCTGGAAT CGGCTCCGAG CTACCAGGCT 
TCAGGAAGGA TCCCAACAAT CCATGCAGAA CTTCATCTTC CTAAGGACAC AGAGGGGCAG 
GTAGATTATG CAGCTATGGT CGATCATTTA CGGGAAACCA ACAATTTACA GGAACAAGCG 
GACATCTTGT ACCTGCTGTA CACCATGAAA GGAGGAGATT GGGACACAAA AATATATGGA 
CAGCCAGGTG TGACCGTTAA GGATCTCCTG AAGGATCTGT ATGGTAAAGT GGGTTCCATG 
CGGCAATGGG CACTGATCCG AGGCATTTCT GGGATACTGA AAAAGAAAGT GGAGGCACTG 
GATGAGGCCT GCACAGACCT TCTGTCCCAT CAGAAGCAAC TAACCGTAGG CCTGCCTCCT 
GAGCCACGGG AAATAGCCAT CACAGCGCCT CTACCTTATG AAAAACTCAT TCGTCTTATT 
GATGAAGCCA GTGAGCAAAA TAGGACCATA TCCATCCTGA CTCAGGAAAT TATGGTGTAT 
CTGGCAATGT ACATGAGGAC CCAGCCATCT CTGTTTGCTG AGATGTTCCG ACTGCGCATT 
GGGCTCATCA TACAGGTCAT GGCTACAGAA CTGTCCCATT CACTCCGTTG CTCTGCTGAG 
GAAGCCACGG AAAGCCTTAT GAACCTCAGC CCGTCTGACA TGAAGAACCT TCTTCACCAT 
ATTCTGAGCG GAAAGGAATT TGGGGTAGAG AGAAGTGTAC GAGGTGGAGA ACCTTGGGTC 
AGTCCAGCGA TTTCTATTCA TGAACTTGGA GCTGTGGGAG CCACAAAGAG TGAAAGAGCA 
GGTATTACCC GACTAAAGAG TGAGATCAAG CTGCTGGATA AACGTAGGCT GTCTCTGGCT 
GCAGAAGGCC AGGTGAAATA TTCCTTCACT GTACATAAAT CACTGCTGAG TTTACACGCG 
GCTGCTGAAA CACCGCATGG GCTCTTCACT TGGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_031890231.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746091.1 Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa63516
Clone ID Related Accession (Same CDS sequence)	XM_031890231.1 , XM_031890232.1
Accession Version	XM_031890231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGGA TGTTGACCCA ACGACTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746091.1
CDS	217..3918
Translation	MRSRSNSGVRLDRYAKLVQQTILCHQNPVTGLLPASSDQKDAWVRDNVYTILAVWGLGLAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVSKVEEFKYSQNTKDALHAKYNTNTCSTVVGDDQWGHLQLDATSIYLLFLAQMTASGLHIIYNLDEVNFIQNLVFYVEAAYKTADFGMWERGDKTNQGITELNASSVGMAKAALESLDELNLFGAKGGPQSVIHVLSDEIQHCQSILHSMLPRASTSKEIDAGVLSVISFPAFAVEDSHLVRITKQEIIDKLQGRYGCCRFLRDGYRTPKEDPGRLYYEPAELKLFENIECEWPLFWTYFILDGIFSSNHEQVQEYKQAIDEVLIKGKSGVRLVPELYSVPEDKVEVEYEHPHTVDRIPLGKLPHMWGQSLYILGCLMSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIQEILKKHGIEAETVAGVYPIRVQPARILSHIYARLGRNRKMNLSGRPYRNMGVLGTSKLYTIRQNIFAFTPQFIDQQQFYLALDNRMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDDSGNDLDPVVLATIRKLQDGYFGGARTQTGFLSKFLNTSCCKHLSFMDAGVEGKLSTWGSVNRGGQPIIVREPDEAPSADDEDVDDEVSLYLNQLLGMSSPKPSPTSQRGGRHRFKAAIHSTRDMLSLRSKAKGLNVQDIQMYLPSKLFLASQPSFDLLESAPSYQASGRIPTIHAELHLPKDTEGQVDYAAMVDHLRETNNLQEQADILYLLYTMKGGDWDTKIYGQPGVTVKDLLKDLYGKVGSMRQWALIRGISGILKKKVEALDEACTDLLSHQKQLTVGLPPEPREIAITAPLPYEKLIRLIDEASEQNRTISILTQEIMVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELSHSLRCSAEEATESLMNLSPSDMKNLLHHILSGKEFGVERSVRGGEPWVSPAISIHELGAVGATKSERAGITRLKSEIKLLDKRRLSLAAEGQDMDLSSGMSLKPGDSLSVSGDLSTSTISDSDSANDSRQGQWLRRRRLDGALNRVPIGFYQKVWLILQKCHGLSVEGFVLPSSSTREMTPGELKFSAHVESVLNRVPQPEYRQLLVEAILVLTMLADVDIQSVGGIIHVEKIVHIANELFCQEQKVLGADDTMLDVDPTTGICHLLYDSAPSGRFGTMTYLSKSVAITLQESLPHSGCSMQ

Target ORF information:

RefSeq Version	XM_031890231.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890231.1

ORF Insert Sequence:

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGGA TGTTGACCCA ACGACTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_031890232.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746092.1 Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X4, mRNA.

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CloneID	OXa63516
Clone ID Related Accession (Same CDS sequence)	XM_031890231.1 , XM_031890232.1
Accession Version	XM_031890232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGGA TGTTGACCCA ACGACTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746092.1
CDS	216..3917
Translation	MRSRSNSGVRLDRYAKLVQQTILCHQNPVTGLLPASSDQKDAWVRDNVYTILAVWGLGLAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVSKVEEFKYSQNTKDALHAKYNTNTCSTVVGDDQWGHLQLDATSIYLLFLAQMTASGLHIIYNLDEVNFIQNLVFYVEAAYKTADFGMWERGDKTNQGITELNASSVGMAKAALESLDELNLFGAKGGPQSVIHVLSDEIQHCQSILHSMLPRASTSKEIDAGVLSVISFPAFAVEDSHLVRITKQEIIDKLQGRYGCCRFLRDGYRTPKEDPGRLYYEPAELKLFENIECEWPLFWTYFILDGIFSSNHEQVQEYKQAIDEVLIKGKSGVRLVPELYSVPEDKVEVEYEHPHTVDRIPLGKLPHMWGQSLYILGCLMSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIQEILKKHGIEAETVAGVYPIRVQPARILSHIYARLGRNRKMNLSGRPYRNMGVLGTSKLYTIRQNIFAFTPQFIDQQQFYLALDNRMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDDSGNDLDPVVLATIRKLQDGYFGGARTQTGFLSKFLNTSCCKHLSFMDAGVEGKLSTWGSVNRGGQPIIVREPDEAPSADDEDVDDEVSLYLNQLLGMSSPKPSPTSQRGGRHRFKAAIHSTRDMLSLRSKAKGLNVQDIQMYLPSKLFLASQPSFDLLESAPSYQASGRIPTIHAELHLPKDTEGQVDYAAMVDHLRETNNLQEQADILYLLYTMKGGDWDTKIYGQPGVTVKDLLKDLYGKVGSMRQWALIRGISGILKKKVEALDEACTDLLSHQKQLTVGLPPEPREIAITAPLPYEKLIRLIDEASEQNRTISILTQEIMVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELSHSLRCSAEEATESLMNLSPSDMKNLLHHILSGKEFGVERSVRGGEPWVSPAISIHELGAVGATKSERAGITRLKSEIKLLDKRRLSLAAEGQDMDLSSGMSLKPGDSLSVSGDLSTSTISDSDSANDSRQGQWLRRRRLDGALNRVPIGFYQKVWLILQKCHGLSVEGFVLPSSSTREMTPGELKFSAHVESVLNRVPQPEYRQLLVEAILVLTMLADVDIQSVGGIIHVEKIVHIANELFCQEQKVLGADDTMLDVDPTTGICHLLYDSAPSGRFGTMTYLSKSVAITLQESLPHSGCSMQ

Target ORF information:

RefSeq Version	XM_031890232.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890232.1

ORF Insert Sequence:

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGGA TGTTGACCCA ACGACTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_031890233.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746093.1 Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X6, mRNA.

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CloneID	OXa63517
Clone ID Related Accession (Same CDS sequence)	XM_031890233.1
Accession Version	XM_031890233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGAA TGTTGATCCA ACGGCTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746093.1
CDS	218..3919
Translation	MRSRSNSGVRLDRYAKLVQQTILCHQNPVTGLLPASSDQKDAWVRDNVYTILAVWGLGLAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVSKVEEFKYSQNTKDALHAKYNTNTCSTVVGDDQWGHLQLDATSIYLLFLAQMTASGLHIIYNLDEVNFIQNLVFYVEAAYKTADFGMWERGDKTNQGITELNASSVGMAKAALESLDELNLFGAKGGPQSVIHVLSDEIQHCQSILHSMLPRASTSKEIDAGVLSVISFPAFAVEDSHLVRITKQEIIDKLQGRYGCCRFLRDGYRTPKEDPGRLYYEPAELKLFENIECEWPLFWTYFILDGIFSSNHEQVQEYKQAIDEVLIKGKSGVRLVPELYSVPEDKVEVEYEHPHTVDRIPLGKLPHMWGQSLYILGCLMSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIQEILKKHGIEAETVAGVYPIRVQPARILSHIYARLGRNRKMNLSGRPYRNMGVLGTSKLYTIRQNIFAFTPQFIDQQQFYLALDNRMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDDSGNDLDPVVLATIRKLQDGYFGGARTQTGFLSKFLNTSCCKHLSFMDAGVEGKLSTWGSVNRGGQPIIVREPDEAPSADDEDVDDEVSLYLNQLLGMSSPKPSPTSQRGGRHRFKAAIHSTRDMLSLRSKAKGLNVQDIQMYLPSKLFLASQPSFDLLESAPSYQASGRIPTIHAELHLPKDTEGQVDYAAMVDHLRETNNLQEQADILYLLYTMKGGDWDTKIYGQPGVTVKDLLKDLYGKVGSMRQWALIRGISGILKKKVEALDEACTDLLSHQKQLTVGLPPEPREIAITAPLPYEKLIRLIDEASEQNRTISILTQEIMVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELSHSLRCSAEEATESLMNLSPSDMKNLLHHILSGKEFGVERSVRGGEPWVSPAISIHELGAVGATKSERAGITRLKSEIKLLDKRRLSLAAEGQDMDLSSGMSLKPGDSLSVSGDLSTSTISDSDSANDSRQGQWLRRRRLDGALNRVPIGFYQKVWLILQKCHGLSVEGFVLPSSSTREMTPGELKFSAHVESVLNRVPQPEYRQLLVEAILVLTMLADVDIQSVGGIIHVEKIVHIANELFCQEQKVLGADDTMLNVDPTAGICHLLYDSAPSGRFGTMTYLSKSVAITLQESLPHSGCSMQ

Target ORF information:

RefSeq Version	XM_031890233.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890233.1

ORF Insert Sequence:

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGG TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGAC AATCTTTATA TATACTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCTGA TGTGGTTGTC 
CAAGTTTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGGTAAGCT GAGCACCTGG GGCAGTGTCA ACCGTGGGGG CCAGCCAATC 
ATCGTGCGGG AGCCAGATGA GGCCCCATCA GCCGATGATG AGGATGTGGA TGACGAAGTG 
TCACTGTATC TTAATCAACT ACTTGGCATG AGCTCCCCCA AACCAAGTCC TACATCACAG 
CGAGGGGGTC GGCATCGCTT TAAAGCTGCC ATTCACAGTA CACGAGATAT GTTATCTCTG 
CGGAGCAAAG CCAAGGGTCT TAATGTGCAA GACATACAGA TGTACCTCCC ATCAAAACTG 
TTCCTGGCAT CACAGCCCTC CTTTGATTTG CTGGAATCGG CTCCGAGCTA CCAGGCTTCA 
GGAAGGATCC CAACAATCCA TGCAGAACTT CATCTTCCTA AGGACACAGA GGGGCAGGTA 
GATTATGCAG CTATGGTCGA TCATTTACGG GAAACCAACA ATTTACAGGA ACAAGCGGAC 
ATCTTGTACC TGCTGTACAC CATGAAAGGA GGAGATTGGG ACACAAAAAT ATATGGACAG 
CCAGGTGTGA CCGTTAAGGA TCTCCTGAAG GATCTGTATG GTAAAGTGGG TTCCATGCGG 
CAATGGGCAC TGATCCGAGG CATTTCTGGG ATACTGAAAA AGAAAGTGGA GGCACTGGAT 
GAGGCCTGCA CAGACCTTCT GTCCCATCAG AAGCAACTAA CCGTAGGCCT GCCTCCTGAG 
CCACGGGAAA TAGCCATCAC AGCGCCTCTA CCTTATGAAA AACTCATTCG TCTTATTGAT 
GAAGCCAGTG AGCAAAATAG GACCATATCC ATCCTGACTC AGGAAATTAT GGTGTATCTG 
GCAATGTACA TGAGGACCCA GCCATCTCTG TTTGCTGAGA TGTTCCGACT GCGCATTGGG 
CTCATCATAC AGGTCATGGC TACAGAACTG TCCCATTCAC TCCGTTGCTC TGCTGAGGAA 
GCCACGGAAA GCCTTATGAA CCTCAGCCCG TCTGACATGA AGAACCTTCT TCACCATATT 
CTGAGCGGAA AGGAATTTGG GGTAGAGAGA AGTGTACGAG GTGGAGAACC TTGGGTCAGT 
CCAGCGATTT CTATTCATGA ACTTGGAGCT GTGGGAGCCA CAAAGAGTGA AAGAGCAGGT 
ATTACCCGAC TAAAGAGTGA GATCAAGCTG CTGGATAAAC GTAGGCTGTC TCTGGCTGCA 
GAAGGCCAGG ACATGGATCT ATCTTCAGGA ATGTCACTGA AACCCGGTGA TTCCCTCTCT 
GTGTCTGGTG ATCTTTCTAC TTCCACTATA TCAGACAGCG ATAGTGCCAA TGATAGCCGC 
CAAGGCCAGT GGCTTCGAAG AAGGCGCCTT GATGGAGCTT TAAACAGAGT GCCTATTGGG 
TTCTACCAGA AAGTTTGGCT AATCCTGCAA AAGTGCCATG GATTGTCTGT AGAAGGTTTT 
GTTTTACCAT CATCAAGCAC GCGTGAGATG ACACCGGGTG AGCTGAAGTT TTCTGCCCAT 
GTGGAATCTG TTTTGAATCG TGTGCCTCAG CCAGAGTACC GCCAGCTCCT AGTGGAAGCT 
ATTTTGGTGC TGACCATGCT TGCTGATGTA GATATCCAGA GTGTTGGGGG TATCATCCAC 
GTGGAAAAGA TTGTGCATAT TGCAAATGAA CTGTTCTGTC AGGAGCAGAA GGTGCTTGGT 
GCGGATGACA CCATGTTGAA TGTTGATCCA ACGGCTGGTA TTTGCCATTT GCTCTATGAC 
AGTGCCCCCA GTGGACGTTT TGGGACAATG ACTTATCTCT CTAAATCAGT AGCCATCACC 
CTGCAGGAGT CCTTACCCCA CAGCGGATGT TCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( NM_001128038.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121510.1 Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.

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CloneID	OXa20534
Clone ID Related Accession (Same CDS sequence)	NM_001128038.1
Accession Version	NM_001128038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166348.1. ##Evidence-Data-START## Transcript exon combination :: BC166348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGA TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGCC AATCTTTATA TATTCTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCCGA TGTGGTTGTC 
CAAGTGTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGAGGATGT GGATGACGAA GTGTCACTGT ATCTTAATCA ACTACTTGGC 
ATGAGCTCCC CCAAACCAAG TCCTACATCA CAGCGAGGGG GTCGGCATCG CTTTAAAGCT 
GCCATTCACA GTACACGAGA TATGTTATCT CTGCGGAGCA AAGCCAAGGG TCTTAATGTG 
CAAGACATAC AGATGTACCT CCCATCAAAA CTGTTCCTGG CATCACAGCC CTCCTTTGAT 
TTGCTGGAAT CGGCTCCGAG CTACCAGGCT TCAGGAAGGA TCCCAACAAT CCATGCAGAA 
CTTCATCTTC CTAAGGACAC AGAGGGGCAG GTAGATTATG CAGCTATGGT CGATCATTTA 
CGGGAAACCA ACAATTTACA GGAACAAGCG GACATCTTGT ACCTGCTGTA CACCATGAAA 
GGAGGAGATT GGGACACAAA AATATATGGA CAGCCAGGTG TGACCGTTAA GGATCTCCTG 
AAGGATCTGT ATGGTAAAGT GGGTTCCATG CGGCAATGGG CACTGATCCG AGGCATTTCT 
GGGATACTGA AAAAGAAAGT GGAGGCACTG GATGAGGCCT GCACAGACCT TCTGTCCCAT 
CAGAAGCAAC TAACCGTAGG CCTGCCTCCT GAGCCACGGG AAATAGCCAT CACAGCGCCT 
CTACCTTATG AAAAACTCAT TCGTCTTATT GATGAAGCCA GTGAGCAAAA TAGGACCATA 
TCCATCCTGA CTCAGGAAAT TATGGTGTAT CTGGCAATGT ACATGAGGAC CCAGCCATCT 
CTGTTTGCTG AGATGTTCCG ACTGCGCATT GGGCTCATCA TACAGGTCAT GGCTACAGAA 
CTGTCCCATT CACTCCGTTG CTCTGCTGAG GAAGCCACGG AAAGCCTTAT GAACCTCAGC 
CCGTCTGACA TGAAGAACCT TCTTCACCAT ATTCTGAGCG GAAAGGAATT TGGGGTAGAG 
AGAAGTGTAC GAGGTGGAGA ACCTTGGGTC AGTCCAGCGA TTTCTATTCA TGAACTTGGA 
GCTGTGGGAG CCACAAAGAG TGAAAGAGCA GGTATTACCC GACTAAAGAG TGAGATCAAG 
CTGGACATGG ATCTATCTTC AGGAATGTCA CTGAAACCCG GCGATTCCCT CTCTGTGTCT 
GGTGATCTTT CTACTTCCAC TATATCAGAC AGCGATAGTG CCAATGATAG CCGCCAAGGC 
CAGTGGCTTC GAAGAAGGCG CCTTGATGGA GCTTTAAACA GAGTGCCTAT TGGGTTCTAC 
CAGAAAGTTT GGCTAATCCT GCAAAAGTGC CATGGATTGT CTGTAGAAGG TTTTGTTTTA 
CCATCATCAA GCACGCGTGA GATGACACCG GGTGAGCTGA AGTTTTCTGC CCATGTGGAA 
TCTGTTTTGA ATCGTGTGCC TCAGCCAGAG TACCGCCAGC TCCTAGTGGA AGCTATTTTG 
GTGCTGACCA TGCTTGCTGA TGTAGATATC CAGAGTGTTG GGGGTATCAT CCATGTGGAA 
AAGATTGTGC ATATTGCAAA TGAACTGTTC TGTCAGGAGC AGAAGGTGCT TGGTGCGGAT 
GACACCATGT TGGATGTTGA CCCAACGACT GGTATTTGCC ATTTGCTCTA TGACAGTGCC 
CCCAGTGGAC GTTTTGGGAC AATGACTTAT CTCTCTAAAT CAGTAGCCAT CACCCTGCAG 
GAGTCCTTAC CCCACAGCGG ATGTTCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121510.1
CDS	128..3703
Translation	MRSRSNSGVRLDRYAKLVQQTILCHQNPVTGLLPASSDQKDAWIRDNVYTILAVWGLGLAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVSKVEEFKYSQNTKDALHAKYNTNTCSTVVGDDQWGHLQLDATSIYLLFLAQMTASGLHIIYNLDEVNFIQNLVFYVEAAYKTADFGMWERGDKTNQGITELNASSVGMAKAALESLDELNLFGAKGGPQSVIHVLSDEIQHCQSILHSMLPRASTSKEIDAGVLSVISFPAFAVEDSHLVRITKQEIIDKLQGRYGCCRFLRDGYRTPKEDPGRLYYEPAELKLFENIECEWPLFWTYFILDGIFSSNHEQVQEYKQAIDEVLIKGKSGVRLVPELYSVPEDKVEVEYEHPHTVDRIPLGKLPHMWGQSLYILGCLMSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIQEILKKHGIEAETVAGVYPIRVQPARILSHIYARLGRNRKMNLSGRPYRNMGVLGTSKLYTIRQNIFAFTPQFIDQQQFYLALDNRMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDDSGNDLDPVVLATIRKLQDGYFGGARTQTGFLSKFLNTSCCKHLSFMDAGVEEDVDDEVSLYLNQLLGMSSPKPSPTSQRGGRHRFKAAIHSTRDMLSLRSKAKGLNVQDIQMYLPSKLFLASQPSFDLLESAPSYQASGRIPTIHAELHLPKDTEGQVDYAAMVDHLRETNNLQEQADILYLLYTMKGGDWDTKIYGQPGVTVKDLLKDLYGKVGSMRQWALIRGISGILKKKVEALDEACTDLLSHQKQLTVGLPPEPREIAITAPLPYEKLIRLIDEASEQNRTISILTQEIMVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELSHSLRCSAEEATESLMNLSPSDMKNLLHHILSGKEFGVERSVRGGEPWVSPAISIHELGAVGATKSERAGITRLKSEIKLDMDLSSGMSLKPGDSLSVSGDLSTSTISDSDSANDSRQGQWLRRRRLDGALNRVPIGFYQKVWLILQKCHGLSVEGFVLPSSSTREMTPGELKFSAHVESVLNRVPQPEYRQLLVEAILVLTMLADVDIQSVGGIIHVEKIVHIANELFCQEQKVLGADDTMLDVDPTTGICHLLYDSAPSGRFGTMTYLSKSVAITLQESLPHSGCSMQ

Target ORF information:

RefSeq Version	NM_001128038.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128038.1

ORF Insert Sequence:

ATGAGGAGCA GGAGTAACTC TGGTGTTCGC CTGGATAGAT ATGCCAAGCT TGTCCAACAG 
ACCATCCTCT GCCACCAGAA TCCAGTCACG GGTCTGTTGC CAGCCAGCTC TGATCAGAAG 
GATGCCTGGA TACGTGACAA CGTGTACACA ATTCTGGCCG TGTGGGGGCT TGGTCTCGCA 
TATCGGAAAA ATGCTGATCG GGATGAAGAT AAAGCAAAGG CATATGAGCT GGAACAGAAT 
GTGGTCAAGC TGATGAGAGG CCTGCTTCAA TGTATGATGA GACAGGTTTC CAAAGTGGAA 
GAGTTTAAAT ATAGCCAGAA TACCAAGGAT GCCCTACATG CTAAATATAA TACCAACACC 
TGCTCCACTG TGGTGGGCGA TGACCAGTGG GGCCACCTAC AGCTTGATGC CACATCCATA 
TACCTGCTGT TCCTGGCACA AATGACTGCA TCAGGCCTGC ACATAATCTA CAATCTGGAT 
GAAGTGAATT TTATACAGAA CCTGGTCTTT TATGTTGAAG CAGCATACAA AACAGCGGAT 
TTTGGAATGT GGGAACGAGG TGACAAAACC AACCAGGGCA TTACTGAGTT GAATGCTAGC 
TCTGTGGGCA TGGCCAAGGC TGCCCTTGAG TCTTTAGATG AGCTGAACTT GTTTGGTGCT 
AAGGGAGGTC CCCAGTCTGT CATACATGTC CTGTCTGATG AAATCCAGCA CTGCCAGTCT 
ATCCTACACT CCATGCTTCC AAGAGCTTCC ACCTCTAAGG AGATAGATGC AGGGGTTCTG 
TCTGTTATCT CATTTCCAGC CTTTGCTGTG GAGGACAGTC ATCTAGTGCG GATTACTAAA 
CAAGAGATCA TTGACAAGTT ACAGGGCCGT TACGGATGCT GTAGGTTTCT CCGAGATGGA 
TACAGAACTC CAAAAGAGGA TCCCGGCAGA CTATATTATG AGCCTGCAGA ACTCAAACTC 
TTTGAAAATA TTGAATGTGA ATGGCCGTTA TTTTGGACCT ATTTTATTCT GGATGGGATA 
TTCAGTAGTA ACCATGAGCA GGTTCAAGAA TACAAGCAAG CCATTGATGA GGTCTTAATA 
AAAGGAAAGT CCGGAGTGAG ACTGGTCCCA GAGCTGTACA GTGTCCCAGA GGACAAAGTG 
GAGGTGGAAT ATGAGCACCC ACACACAGTA GATCGCATAC CCTTGGGGAA GCTGCCACAC 
ATGTGGGGCC AATCTTTATA TATTCTGGGA TGTCTAATGT CTGAGGGCTT CCTGGCTCCT 
GGGGAGATTG ATCCCCTGAA CAGGAGATTC TCTACAGTCC CTAAGCCCGA TGTGGTTGTC 
CAAGTGTCTA TCCTAGCAGA AACCGATGAG ATTCAAGAAA TATTGAAGAA GCATGGAATT 
GAAGCAGAGA CAGTTGCTGG AGTGTACCCA ATTAGAGTGC AGCCGGCCAG GATTCTGAGT 
CACATCTATG CACGGCTTGG TCGTAACCGG AAAATGAATC TCAGTGGAAG ACCCTACAGA 
AATATGGGCG TTCTGGGTAC CTCAAAGCTC TACACCATTC GCCAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGATCA GCAGCAGTTC TATCTGGCTT TGGATAACCG GATGATTGTG 
GAGATGTTGC GCACTGACCT ATCTTACCTG TGCAGTCGCT GGAGAATGAC CGGCCGCCCA 
ACCATCACCT TCCCAATCTC TCACACTATG CTAGATGATT CTGGTAATGA CCTTGACCCA 
GTTGTCCTTG CCACAATCAG AAAACTACAG GATGGATATT TTGGGGGTGC CAGGACCCAG 
ACCGGCTTCC TGTCGAAATT CCTAAACACA TCCTGCTGCA AACACCTGAG CTTCATGGAT 
GCCGGTGTGG AGGAGGATGT GGATGACGAA GTGTCACTGT ATCTTAATCA ACTACTTGGC 
ATGAGCTCCC CCAAACCAAG TCCTACATCA CAGCGAGGGG GTCGGCATCG CTTTAAAGCT 
GCCATTCACA GTACACGAGA TATGTTATCT CTGCGGAGCA AAGCCAAGGG TCTTAATGTG 
CAAGACATAC AGATGTACCT CCCATCAAAA CTGTTCCTGG CATCACAGCC CTCCTTTGAT 
TTGCTGGAAT CGGCTCCGAG CTACCAGGCT TCAGGAAGGA TCCCAACAAT CCATGCAGAA 
CTTCATCTTC CTAAGGACAC AGAGGGGCAG GTAGATTATG CAGCTATGGT CGATCATTTA 
CGGGAAACCA ACAATTTACA GGAACAAGCG GACATCTTGT ACCTGCTGTA CACCATGAAA 
GGAGGAGATT GGGACACAAA AATATATGGA CAGCCAGGTG TGACCGTTAA GGATCTCCTG 
AAGGATCTGT ATGGTAAAGT GGGTTCCATG CGGCAATGGG CACTGATCCG AGGCATTTCT 
GGGATACTGA AAAAGAAAGT GGAGGCACTG GATGAGGCCT GCACAGACCT TCTGTCCCAT 
CAGAAGCAAC TAACCGTAGG CCTGCCTCCT GAGCCACGGG AAATAGCCAT CACAGCGCCT 
CTACCTTATG AAAAACTCAT TCGTCTTATT GATGAAGCCA GTGAGCAAAA TAGGACCATA 
TCCATCCTGA CTCAGGAAAT TATGGTGTAT CTGGCAATGT ACATGAGGAC CCAGCCATCT 
CTGTTTGCTG AGATGTTCCG ACTGCGCATT GGGCTCATCA TACAGGTCAT GGCTACAGAA 
CTGTCCCATT CACTCCGTTG CTCTGCTGAG GAAGCCACGG AAAGCCTTAT GAACCTCAGC 
CCGTCTGACA TGAAGAACCT TCTTCACCAT ATTCTGAGCG GAAAGGAATT TGGGGTAGAG 
AGAAGTGTAC GAGGTGGAGA ACCTTGGGTC AGTCCAGCGA TTTCTATTCA TGAACTTGGA 
GCTGTGGGAG CCACAAAGAG TGAAAGAGCA GGTATTACCC GACTAAAGAG TGAGATCAAG 
CTGGACATGG ATCTATCTTC AGGAATGTCA CTGAAACCCG GCGATTCCCT CTCTGTGTCT 
GGTGATCTTT CTACTTCCAC TATATCAGAC AGCGATAGTG CCAATGATAG CCGCCAAGGC 
CAGTGGCTTC GAAGAAGGCG CCTTGATGGA GCTTTAAACA GAGTGCCTAT TGGGTTCTAC 
CAGAAAGTTT GGCTAATCCT GCAAAAGTGC CATGGATTGT CTGTAGAAGG TTTTGTTTTA 
CCATCATCAA GCACGCGTGA GATGACACCG GGTGAGCTGA AGTTTTCTGC CCATGTGGAA 
TCTGTTTTGA ATCGTGTGCC TCAGCCAGAG TACCGCCAGC TCCTAGTGGA AGCTATTTTG 
GTGCTGACCA TGCTTGCTGA TGTAGATATC CAGAGTGTTG GGGGTATCAT CCATGTGGAA 
AAGATTGTGC ATATTGCAAA TGAACTGTTC TGTCAGGAGC AGAAGGTGCT TGGTGCGGAT 
GACACCATGT TGGATGTTGA CCCAACGACT GGTATTTGCC ATTTGCTCTA TGACAGTGCC 
CCCAGTGGAC GTTTTGGGAC AATGACTTAT CTCTCTAAAT CAGTAGCCAT CACCCTGCAG 
GAGTCCTTAC CCCACAGCGG ATGTTCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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phka1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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