PHKA1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PHKA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285457 XM_022416330.1
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45574 XM_014111594.1
Latest version!
Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe32553 XM_005641494.2
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32554 XM_854169.4
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32555 XM_549076.5
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32556 XM_005641495.2
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32557 XM_854209.4
Latest version!
Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32558 XM_005641496.2
Latest version!
Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe32557 XM_854209.3 Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32556 XM_005641495.1 Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32555 XM_549076.4 Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32553 XM_005641494.1 Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32554 XM_854169.3 Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285457
    Clone ID Related Accession (Same CDS sequence) XM_022416330.1
    Accession Version XM_022416330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGAAAAC CCTCGGTGCA
    GATGATACCA TGTTGGCAAA GGATCCTGCG TCTGGCATCT GTACTCTTCT GTATGACAGC
    GCACCCAGTG GCAGGTTTGG CACCATGACC TACCTCTCCA AGGCAGCTGC CACCTACGTA
    CAGGAATTCC TGCCCCACAG CATCTGTGCC ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272038.1
    CDS409..3927
    Translation

    Target ORF information:

    RefSeq Version XM_022416330.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGAAAAC CCTCGGTGCA
    GATGATACCA TGTTGGCAAA GGATCCTGCG TCTGGCATCT GTACTCTTCT GTATGACAGC
    GCACCCAGTG GCAGGTTTGG CACCATGACC TACCTCTCCA AGGCAGCTGC CACCTACGTA
    CAGGAATTCC TGCCCCACAG CATCTGTGCC ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45574
    Clone ID Related Accession (Same CDS sequence) XM_014111594.1
    Accession Version XM_014111594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGGCTTGTC TTATAGCCCA TAAAAGCAAC AAACTAAACC ATAGACCAGC TAGAAGTTTA
    ACAGAGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967069.1
    CDS392..3583
    Translation

    Target ORF information:

    RefSeq Version XM_014111594.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGGCTTGTC TTATAGCCCA TAAAAGCAAC AAACTAAACC ATAGACCAGC TAGAAGTTTA
    ACAGAGAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32553
    Clone ID Related Accession (Same CDS sequence) XM_005641494.2 , XM_005641494.1
    Accession Version XM_005641494.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGATGAC TCCAGGCGAG
    ATTAAATTCT CTGTTCATGT GGAGTCTGTC CTGAATCGTG TACCTCAGCC TGAGTACCGC
    CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC ACCATGATGG CAGATATTGA AATTCATAGT
    ATCGGAAGCA TCATTGCTGT GGAAAAAATA GTGCATATTG CTAATGACTT GTTCCTTCAA
    GAACAGAAAA CCCTCGGTGC AGATGATACC ATGTTGGCAA AGGATCCTGC GTCTGGCATC
    TGTACTCTTC TGTATGACAG CGCACCCAGT GGCAGGTTTG GCACCATGAC CTACCTCTCC
    AAGGCAGCTG CCACCTACGT ACAGGAATTC CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641551.1
    CDS409..4128
    Translation

    Target ORF information:

    RefSeq Version XM_005641494.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641494.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGATGAC TCCAGGCGAG
    ATTAAATTCT CTGTTCATGT GGAGTCTGTC CTGAATCGTG TACCTCAGCC TGAGTACCGC
    CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC ACCATGATGG CAGATATTGA AATTCATAGT
    ATCGGAAGCA TCATTGCTGT GGAAAAAATA GTGCATATTG CTAATGACTT GTTCCTTCAA
    GAACAGAAAA CCCTCGGTGC AGATGATACC ATGTTGGCAA AGGATCCTGC GTCTGGCATC
    TGTACTCTTC TGTATGACAG CGCACCCAGT GGCAGGTTTG GCACCATGAC CTACCTCTCC
    AAGGCAGCTG CCACCTACGT ACAGGAATTC CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32553
    Clone ID Related Accession (Same CDS sequence) XM_005641494.2 , XM_005641494.1
    Accession Version XM_005641494.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGATGAC TCCAGGCGAG
    ATTAAATTCT CTGTTCATGT GGAGTCTGTC CTGAATCGTG TACCTCAGCC TGAGTACCGC
    CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC ACCATGATGG CAGATATTGA AATTCATAGT
    ATCGGAAGCA TCATTGCTGT GGAAAAAATA GTGCATATTG CTAATGACTT GTTCCTTCAA
    GAACAGAAAA CCCTCGGTGC AGATGATACC ATGTTGGCAA AGGATCCTGC GTCTGGCATC
    TGTACTCTTC TGTATGACAG CGCACCCAGT GGCAGGTTTG GCACCATGAC CTACCTCTCC
    AAGGCAGCTG CCACCTACGT ACAGGAATTC CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641551.1
    CDS392..4111
    Translation

    Target ORF information:

    RefSeq Version XM_005641494.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGTCACCTG GACCATCTGT GGCTCCAAGT ATTGGGTCCT TTCCTAGTGC ATATGGTCAA
    CAGACATCTA AAGATCGTCA AGGTCAGTGG CAACGCCGAA GGCGGCTAGA TGGAGCACTG
    AACAGAGTCC CAGTTGGATT TTATCAAAAA GTATGGAAAG TTTTACAGAA GTGTCATGGA
    CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT TCAACCACTA GAGAGATGAC TCCAGGCGAG
    ATTAAATTCT CTGTTCATGT GGAGTCTGTC CTGAATCGTG TACCTCAGCC TGAGTACCGC
    CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC ACCATGATGG CAGATATTGA AATTCATAGT
    ATCGGAAGCA TCATTGCTGT GGAAAAAATA GTGCATATTG CTAATGACTT GTTCCTTCAA
    GAACAGAAAA CCCTCGGTGC AGATGATACC ATGTTGGCAA AGGATCCTGC GTCTGGCATC
    TGTACTCTTC TGTATGACAG CGCACCCAGT GGCAGGTTTG GCACCATGAC CTACCTCTCC
    AAGGCAGCTG CCACCTACGT ACAGGAATTC CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32554
    Clone ID Related Accession (Same CDS sequence) XM_854169.4 , XM_854169.3
    Accession Version XM_854169.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_854169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCACCTGG ACCATCTGTG GCTCCAAGTA TTGGGTCCTT TCCTAGTGCA
    TATGGTCAAC AGACATCTAA AGATCGTCAA GGTCAGTGGC AACGCCGAAG GCGGCTAGAT
    GGAGCACTGA ACAGAGTCCC AGTTGGATTT TATCAAAAAG TATGGAAAGT TTTACAGAAG
    TGTCATGGAC TCTCTGTGGA AGGTTTTGTT CTTCCTTCTT CAACCACTAG AGAGATGACT
    CCAGGCGAGA TTAAATTCTC TGTTCATGTG GAGTCTGTCC TGAATCGTGT ACCTCAGCCT
    GAGTACCGCC AGCTTCTGGT AGAAGCCATC CTTGTCCTCA CCATGATGGC AGATATTGAA
    ATTCATAGTA TCGGAAGCAT CATTGCTGTG GAAAAAATAG TGCATATTGC TAATGACTTG
    TTCCTTCAAG AACAGAAAAC CCTCGGTGCA GATGATACCA TGTTGGCAAA GGATCCTGCG
    TCTGGCATCT GTACTCTTCT GTATGACAGC GCACCCAGTG GCAGGTTTGG CACCATGACC
    TACCTCTCCA AGGCAGCTGC CACCTACGTA CAGGAATTCC TGCCCCACAG CATCTGTGCC
    ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859262.1
    CDS409..4077
    Translation

    Target ORF information:

    RefSeq Version XM_854169.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854169.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCACCTGG ACCATCTGTG GCTCCAAGTA TTGGGTCCTT TCCTAGTGCA
    TATGGTCAAC AGACATCTAA AGATCGTCAA GGTCAGTGGC AACGCCGAAG GCGGCTAGAT
    GGAGCACTGA ACAGAGTCCC AGTTGGATTT TATCAAAAAG TATGGAAAGT TTTACAGAAG
    TGTCATGGAC TCTCTGTGGA AGGTTTTGTT CTTCCTTCTT CAACCACTAG AGAGATGACT
    CCAGGCGAGA TTAAATTCTC TGTTCATGTG GAGTCTGTCC TGAATCGTGT ACCTCAGCCT
    GAGTACCGCC AGCTTCTGGT AGAAGCCATC CTTGTCCTCA CCATGATGGC AGATATTGAA
    ATTCATAGTA TCGGAAGCAT CATTGCTGTG GAAAAAATAG TGCATATTGC TAATGACTTG
    TTCCTTCAAG AACAGAAAAC CCTCGGTGCA GATGATACCA TGTTGGCAAA GGATCCTGCG
    TCTGGCATCT GTACTCTTCT GTATGACAGC GCACCCAGTG GCAGGTTTGG CACCATGACC
    TACCTCTCCA AGGCAGCTGC CACCTACGTA CAGGAATTCC TGCCCCACAG CATCTGTGCC
    ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32554
    Clone ID Related Accession (Same CDS sequence) XM_854169.4 , XM_854169.3
    Accession Version XM_854169.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_854169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCACCTGG ACCATCTGTG GCTCCAAGTA TTGGGTCCTT TCCTAGTGCA
    TATGGTCAAC AGACATCTAA AGATCGTCAA GGTCAGTGGC AACGCCGAAG GCGGCTAGAT
    GGAGCACTGA ACAGAGTCCC AGTTGGATTT TATCAAAAAG TATGGAAAGT TTTACAGAAG
    TGTCATGGAC TCTCTGTGGA AGGTTTTGTT CTTCCTTCTT CAACCACTAG AGAGATGACT
    CCAGGCGAGA TTAAATTCTC TGTTCATGTG GAGTCTGTCC TGAATCGTGT ACCTCAGCCT
    GAGTACCGCC AGCTTCTGGT AGAAGCCATC CTTGTCCTCA CCATGATGGC AGATATTGAA
    ATTCATAGTA TCGGAAGCAT CATTGCTGTG GAAAAAATAG TGCATATTGC TAATGACTTG
    TTCCTTCAAG AACAGAAAAC CCTCGGTGCA GATGATACCA TGTTGGCAAA GGATCCTGCG
    TCTGGCATCT GTACTCTTCT GTATGACAGC GCACCCAGTG GCAGGTTTGG CACCATGACC
    TACCTCTCCA AGGCAGCTGC CACCTACGTA CAGGAATTCC TGCCCCACAG CATCTGTGCC
    ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859262.1
    CDS392..4060
    Translation

    Target ORF information:

    RefSeq Version XM_854169.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCACCTGG ACCATCTGTG GCTCCAAGTA TTGGGTCCTT TCCTAGTGCA
    TATGGTCAAC AGACATCTAA AGATCGTCAA GGTCAGTGGC AACGCCGAAG GCGGCTAGAT
    GGAGCACTGA ACAGAGTCCC AGTTGGATTT TATCAAAAAG TATGGAAAGT TTTACAGAAG
    TGTCATGGAC TCTCTGTGGA AGGTTTTGTT CTTCCTTCTT CAACCACTAG AGAGATGACT
    CCAGGCGAGA TTAAATTCTC TGTTCATGTG GAGTCTGTCC TGAATCGTGT ACCTCAGCCT
    GAGTACCGCC AGCTTCTGGT AGAAGCCATC CTTGTCCTCA CCATGATGGC AGATATTGAA
    ATTCATAGTA TCGGAAGCAT CATTGCTGTG GAAAAAATAG TGCATATTGC TAATGACTTG
    TTCCTTCAAG AACAGAAAAC CCTCGGTGCA GATGATACCA TGTTGGCAAA GGATCCTGCG
    TCTGGCATCT GTACTCTTCT GTATGACAGC GCACCCAGTG GCAGGTTTGG CACCATGACC
    TACCTCTCCA AGGCAGCTGC CACCTACGTA CAGGAATTCC TGCCCCACAG CATCTGTGCC
    ATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32555
    Clone ID Related Accession (Same CDS sequence) XM_549076.4 , XM_549076.5
    Accession Version XM_549076.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_549076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGTCACCTG GACCATCTGT GGCTCCAAGT
    ATTGGGTCCT TTCCTAGTGC ATATGGTCAA CAGACATCTA AAGATCGTCA AGGTCAGTGG
    CAACGCCGAA GGCGGCTAGA TGGAGCACTG AACAGAGTCC CAGTTGGATT TTATCAAAAA
    GTATGGAAAG TTTTACAGAA GTGTCATGGA CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT
    TCAACCACTA GAGAGATGAC TCCAGGCGAG ATTAAATTCT CTGTTCATGT GGAGTCTGTC
    CTGAATCGTG TACCTCAGCC TGAGTACCGC CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC
    ACCATGATGG CAGATATTGA AATTCATAGT ATCGGAAGCA TCATTGCTGT GGAAAAAATA
    GTGCATATTG CTAATGACTT GTTCCTTCAA GAACAGAAAA CCCTCGGTGC AGATGATACC
    ATGTTGGCAA AGGATCCTGC GTCTGGCATC TGTACTCTTC TGTATGACAG CGCACCCAGT
    GGCAGGTTTG GCACCATGAC CTACCTCTCC AAGGCAGCTG CCACCTACGT ACAGGAATTC
    CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_549076.2
    CDS392..4021
    Translation

    Target ORF information:

    RefSeq Version XM_549076.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_549076.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGTCACCTG GACCATCTGT GGCTCCAAGT
    ATTGGGTCCT TTCCTAGTGC ATATGGTCAA CAGACATCTA AAGATCGTCA AGGTCAGTGG
    CAACGCCGAA GGCGGCTAGA TGGAGCACTG AACAGAGTCC CAGTTGGATT TTATCAAAAA
    GTATGGAAAG TTTTACAGAA GTGTCATGGA CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT
    TCAACCACTA GAGAGATGAC TCCAGGCGAG ATTAAATTCT CTGTTCATGT GGAGTCTGTC
    CTGAATCGTG TACCTCAGCC TGAGTACCGC CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC
    ACCATGATGG CAGATATTGA AATTCATAGT ATCGGAAGCA TCATTGCTGT GGAAAAAATA
    GTGCATATTG CTAATGACTT GTTCCTTCAA GAACAGAAAA CCCTCGGTGC AGATGATACC
    ATGTTGGCAA AGGATCCTGC GTCTGGCATC TGTACTCTTC TGTATGACAG CGCACCCAGT
    GGCAGGTTTG GCACCATGAC CTACCTCTCC AAGGCAGCTG CCACCTACGT ACAGGAATTC
    CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32555
    Clone ID Related Accession (Same CDS sequence) XM_549076.4 , XM_549076.5
    Accession Version XM_549076.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_549076.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGTCACCTG GACCATCTGT GGCTCCAAGT
    ATTGGGTCCT TTCCTAGTGC ATATGGTCAA CAGACATCTA AAGATCGTCA AGGTCAGTGG
    CAACGCCGAA GGCGGCTAGA TGGAGCACTG AACAGAGTCC CAGTTGGATT TTATCAAAAA
    GTATGGAAAG TTTTACAGAA GTGTCATGGA CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT
    TCAACCACTA GAGAGATGAC TCCAGGCGAG ATTAAATTCT CTGTTCATGT GGAGTCTGTC
    CTGAATCGTG TACCTCAGCC TGAGTACCGC CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC
    ACCATGATGG CAGATATTGA AATTCATAGT ATCGGAAGCA TCATTGCTGT GGAAAAAATA
    GTGCATATTG CTAATGACTT GTTCCTTCAA GAACAGAAAA CCCTCGGTGC AGATGATACC
    ATGTTGGCAA AGGATCCTGC GTCTGGCATC TGTACTCTTC TGTATGACAG CGCACCCAGT
    GGCAGGTTTG GCACCATGAC CTACCTCTCC AAGGCAGCTG CCACCTACGT ACAGGAATTC
    CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_549076.2
    CDS409..4038
    Translation

    Target ORF information:

    RefSeq Version XM_549076.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_549076.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGTCACCTG GACCATCTGT GGCTCCAAGT
    ATTGGGTCCT TTCCTAGTGC ATATGGTCAA CAGACATCTA AAGATCGTCA AGGTCAGTGG
    CAACGCCGAA GGCGGCTAGA TGGAGCACTG AACAGAGTCC CAGTTGGATT TTATCAAAAA
    GTATGGAAAG TTTTACAGAA GTGTCATGGA CTCTCTGTGG AAGGTTTTGT TCTTCCTTCT
    TCAACCACTA GAGAGATGAC TCCAGGCGAG ATTAAATTCT CTGTTCATGT GGAGTCTGTC
    CTGAATCGTG TACCTCAGCC TGAGTACCGC CAGCTTCTGG TAGAAGCCAT CCTTGTCCTC
    ACCATGATGG CAGATATTGA AATTCATAGT ATCGGAAGCA TCATTGCTGT GGAAAAAATA
    GTGCATATTG CTAATGACTT GTTCCTTCAA GAACAGAAAA CCCTCGGTGC AGATGATACC
    ATGTTGGCAA AGGATCCTGC GTCTGGCATC TGTACTCTTC TGTATGACAG CGCACCCAGT
    GGCAGGTTTG GCACCATGAC CTACCTCTCC AAGGCAGCTG CCACCTACGT ACAGGAATTC
    CTGCCCCACA GCATCTGTGC CATGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32556
    Clone ID Related Accession (Same CDS sequence) XM_005641495.1 , XM_005641495.2
    Accession Version XM_005641495.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTATCTGTC
    TCAACTGAGA GTCAGTCCAA TGGTGGTCAT CCCTTGGGTA TAGATTTGAT GTCACCGTCT
    TTTCTGTCAC CTGGACCATC TGTGGCTCCA AGTATTGGGT CCTTTCCTAG TGCATATGGT
    CAACAGACAT CTAAAGATCG TCAAGGTCAG TGGCAACGCC GAAGGCGGCT AGATGGAGCA
    CTGAACAGAG TCCCAGTTGG ATTTTATCAA AAAGTATGGA AAGTTTTACA GAAGTGTCAT
    GGACTCTCTG TGGAAGGTTT TGTTCTTCCT TCTTCAACCA CTAGAGAGAT GACTCCAGGC
    GAGATTAAAT TCTCTGTTCA TGTGGAGTCT GTCCTGAATC GTGTACCTCA GCCTGAGTAC
    CGCCAGCTTC TGGTAGAAGC CATCCTTGTC CTCACCATGA TGGCAGATAT TGAAATTCAT
    AGTATCGGAA GCATCATTGC TGTGGAAAAA ATAGTGCATA TTGCTAATGA CTTGTTCCTT
    CAAGAACAGA AAACCCTCGG TGCAGATGAT ACCATGTTGG CAAAGGATCC TGCGTCTGGC
    ATCTGTACTC TTCTGTATGA CAGCGCACCC AGTGGCAGGT TTGGCACCAT GACCTACCTC
    TCCAAGGCAG CTGCCACCTA CGTACAGGAA TTCCTGCCCC ACAGCATCTG TGCCATGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641552.1
    CDS392..3934
    Translation

    Target ORF information:

    RefSeq Version XM_005641495.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTATCTGTC
    TCAACTGAGA GTCAGTCCAA TGGTGGTCAT CCCTTGGGTA TAGATTTGAT GTCACCGTCT
    TTTCTGTCAC CTGGACCATC TGTGGCTCCA AGTATTGGGT CCTTTCCTAG TGCATATGGT
    CAACAGACAT CTAAAGATCG TCAAGGTCAG TGGCAACGCC GAAGGCGGCT AGATGGAGCA
    CTGAACAGAG TCCCAGTTGG ATTTTATCAA AAAGTATGGA AAGTTTTACA GAAGTGTCAT
    GGACTCTCTG TGGAAGGTTT TGTTCTTCCT TCTTCAACCA CTAGAGAGAT GACTCCAGGC
    GAGATTAAAT TCTCTGTTCA TGTGGAGTCT GTCCTGAATC GTGTACCTCA GCCTGAGTAC
    CGCCAGCTTC TGGTAGAAGC CATCCTTGTC CTCACCATGA TGGCAGATAT TGAAATTCAT
    AGTATCGGAA GCATCATTGC TGTGGAAAAA ATAGTGCATA TTGCTAATGA CTTGTTCCTT
    CAAGAACAGA AAACCCTCGG TGCAGATGAT ACCATGTTGG CAAAGGATCC TGCGTCTGGC
    ATCTGTACTC TTCTGTATGA CAGCGCACCC AGTGGCAGGT TTGGCACCAT GACCTACCTC
    TCCAAGGCAG CTGCCACCTA CGTACAGGAA TTCCTGCCCC ACAGCATCTG TGCCATGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32556
    Clone ID Related Accession (Same CDS sequence) XM_005641495.1 , XM_005641495.2
    Accession Version XM_005641495.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTATCTGTC
    TCAACTGAGA GTCAGTCCAA TGGTGGTCAT CCCTTGGGTA TAGATTTGAT GTCACCGTCT
    TTTCTGTCAC CTGGACCATC TGTGGCTCCA AGTATTGGGT CCTTTCCTAG TGCATATGGT
    CAACAGACAT CTAAAGATCG TCAAGGTCAG TGGCAACGCC GAAGGCGGCT AGATGGAGCA
    CTGAACAGAG TCCCAGTTGG ATTTTATCAA AAAGTATGGA AAGTTTTACA GAAGTGTCAT
    GGACTCTCTG TGGAAGGTTT TGTTCTTCCT TCTTCAACCA CTAGAGAGAT GACTCCAGGC
    GAGATTAAAT TCTCTGTTCA TGTGGAGTCT GTCCTGAATC GTGTACCTCA GCCTGAGTAC
    CGCCAGCTTC TGGTAGAAGC CATCCTTGTC CTCACCATGA TGGCAGATAT TGAAATTCAT
    AGTATCGGAA GCATCATTGC TGTGGAAAAA ATAGTGCATA TTGCTAATGA CTTGTTCCTT
    CAAGAACAGA AAACCCTCGG TGCAGATGAT ACCATGTTGG CAAAGGATCC TGCGTCTGGC
    ATCTGTACTC TTCTGTATGA CAGCGCACCC AGTGGCAGGT TTGGCACCAT GACCTACCTC
    TCCAAGGCAG CTGCCACCTA CGTACAGGAA TTCCTGCCCC ACAGCATCTG TGCCATGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641552.1
    CDS409..3951
    Translation

    Target ORF information:

    RefSeq Version XM_005641495.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG ACTATCTGTC
    TCAACTGAGA GTCAGTCCAA TGGTGGTCAT CCCTTGGGTA TAGATTTGAT GTCACCGTCT
    TTTCTGTCAC CTGGACCATC TGTGGCTCCA AGTATTGGGT CCTTTCCTAG TGCATATGGT
    CAACAGACAT CTAAAGATCG TCAAGGTCAG TGGCAACGCC GAAGGCGGCT AGATGGAGCA
    CTGAACAGAG TCCCAGTTGG ATTTTATCAA AAAGTATGGA AAGTTTTACA GAAGTGTCAT
    GGACTCTCTG TGGAAGGTTT TGTTCTTCCT TCTTCAACCA CTAGAGAGAT GACTCCAGGC
    GAGATTAAAT TCTCTGTTCA TGTGGAGTCT GTCCTGAATC GTGTACCTCA GCCTGAGTAC
    CGCCAGCTTC TGGTAGAAGC CATCCTTGTC CTCACCATGA TGGCAGATAT TGAAATTCAT
    AGTATCGGAA GCATCATTGC TGTGGAAAAA ATAGTGCATA TTGCTAATGA CTTGTTCCTT
    CAAGAACAGA AAACCCTCGG TGCAGATGAT ACCATGTTGG CAAAGGATCC TGCGTCTGGC
    ATCTGTACTC TTCTGTATGA CAGCGCACCC AGTGGCAGGT TTGGCACCAT GACCTACCTC
    TCCAAGGCAG CTGCCACCTA CGTACAGGAA TTCCTGCCCC ACAGCATCTG TGCCATGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32557
    Clone ID Related Accession (Same CDS sequence) XM_854209.4 , XM_854209.3
    Accession Version XM_854209.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_854209.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGTCAC CTGGACCATC TGTGGCTCCA
    AGTATTGGGT CCTTTCCTAG TGCATATGGT CAACAGACAT CTAAAGATCG TCAAGGTCAG
    TGGCAACGCC GAAGGCGGCT AGATGGAGCA CTGAACAGAG TCCCAGTTGG ATTTTATCAA
    AAAGTATGGA AAGTTTTACA GAAGTGTCAT GGACTCTCTG TGGAAGGTTT TGTTCTTCCT
    TCTTCAACCA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
    GTCCTGAATC GTGTACCTCA GCCTGAGTAC CGCCAGCTTC TGGTAGAAGC CATCCTTGTC
    CTCACCATGA TGGCAGATAT TGAAATTCAT AGTATCGGAA GCATCATTGC TGTGGAAAAA
    ATAGTGCATA TTGCTAATGA CTTGTTCCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT
    ACCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGCGCACCC
    AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CTGCCACCTA CGTACAGGAA
    TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859302.2
    CDS409..3861
    Translation

    Target ORF information:

    RefSeq Version XM_854209.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854209.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGTCAC CTGGACCATC TGTGGCTCCA
    AGTATTGGGT CCTTTCCTAG TGCATATGGT CAACAGACAT CTAAAGATCG TCAAGGTCAG
    TGGCAACGCC GAAGGCGGCT AGATGGAGCA CTGAACAGAG TCCCAGTTGG ATTTTATCAA
    AAAGTATGGA AAGTTTTACA GAAGTGTCAT GGACTCTCTG TGGAAGGTTT TGTTCTTCCT
    TCTTCAACCA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
    GTCCTGAATC GTGTACCTCA GCCTGAGTAC CGCCAGCTTC TGGTAGAAGC CATCCTTGTC
    CTCACCATGA TGGCAGATAT TGAAATTCAT AGTATCGGAA GCATCATTGC TGTGGAAAAA
    ATAGTGCATA TTGCTAATGA CTTGTTCCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT
    ACCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGCGCACCC
    AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CTGCCACCTA CGTACAGGAA
    TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32557
    Clone ID Related Accession (Same CDS sequence) XM_854209.4 , XM_854209.3
    Accession Version XM_854209.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_854209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGTCAC CTGGACCATC TGTGGCTCCA
    AGTATTGGGT CCTTTCCTAG TGCATATGGT CAACAGACAT CTAAAGATCG TCAAGGTCAG
    TGGCAACGCC GAAGGCGGCT AGATGGAGCA CTGAACAGAG TCCCAGTTGG ATTTTATCAA
    AAAGTATGGA AAGTTTTACA GAAGTGTCAT GGACTCTCTG TGGAAGGTTT TGTTCTTCCT
    TCTTCAACCA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
    GTCCTGAATC GTGTACCTCA GCCTGAGTAC CGCCAGCTTC TGGTAGAAGC CATCCTTGTC
    CTCACCATGA TGGCAGATAT TGAAATTCAT AGTATCGGAA GCATCATTGC TGTGGAAAAA
    ATAGTGCATA TTGCTAATGA CTTGTTCCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT
    ACCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGCGCACCC
    AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CTGCCACCTA CGTACAGGAA
    TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_859302.2
    CDS392..3844
    Translation

    Target ORF information:

    RefSeq Version XM_854209.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_854209.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG ATGTTCACAT GTATCTTCCA
    ACAAAGATAT TTCAGGCTTC TCGGCCTTCG TTCAACTTAC TTGACTCACC TCACTGCCCA
    CGAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CCAGTCTGGG
    GAGTTGGACT TCAAAGCACT GGTTTTACAG TTGAAGGAGA CCTCCAGCTT ACAGGAGCAA
    GCTGATATCC TTTACATGCT GTATACTATG AAAGGACCTG ACTGGGACAC TGAATTATAT
    GATGAAGAAA GTGCAACAGT CCAAGAGCTG CTTACTGAAC TGTATGGCAA AGTTGGAGAA
    ATTCGTCACT GGGGCCTGAT CCGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA
    CTTGATGAGG CCTGCACAGC CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCT
    CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTGCT CACTCGGCTG
    ATAGATGAGG CTAGTGAAGG GGACATGAGC ATCTCAATCC TTACACAGGA AATAATGGTA
    TATCTAGCCA TGTATATGCG AACTCAGCCT GGCCTCTTCG CTGAAATGTT TCGACTTCGA
    ATTGGTCTAA TCATACAAGT TATGGCAACA GAACTGGCCC ATTCCCTTCG ATGCTCAGCT
    GAGGAAGCCA CAGACAGCCT AATGAATCTT AGTCCTTCAG CCATGAAGAA CCTCCTGCAT
    CACATTCTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCCAC TGATTCAAAT
    GTTAGCCCTG CTATTTCTAT CCATGAGATT GGTGCTGTTG GAGCAACCAA AACAGAACGA
    ACTGGAATAA TGCAATTAAA AAGTGAGATA AAGCAGTCAC CTGGACCATC TGTGGCTCCA
    AGTATTGGGT CCTTTCCTAG TGCATATGGT CAACAGACAT CTAAAGATCG TCAAGGTCAG
    TGGCAACGCC GAAGGCGGCT AGATGGAGCA CTGAACAGAG TCCCAGTTGG ATTTTATCAA
    AAAGTATGGA AAGTTTTACA GAAGTGTCAT GGACTCTCTG TGGAAGGTTT TGTTCTTCCT
    TCTTCAACCA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT
    GTCCTGAATC GTGTACCTCA GCCTGAGTAC CGCCAGCTTC TGGTAGAAGC CATCCTTGTC
    CTCACCATGA TGGCAGATAT TGAAATTCAT AGTATCGGAA GCATCATTGC TGTGGAAAAA
    ATAGTGCATA TTGCTAATGA CTTGTTCCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT
    ACCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGCGCACCC
    AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAGGCAG CTGCCACCTA CGTACAGGAA
    TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32558
    Clone ID Related Accession (Same CDS sequence) XM_005641496.2
    Accession Version XM_005641496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGCCCATAA AAGCAACAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641553.1
    CDS392..3535
    Translation

    Target ORF information:

    RefSeq Version XM_005641496.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGAGCC GGAGTAACTC TGGTGTCCGA CTGGATGGCT ACGCCCGGCT TGTACATCAG 
    ACCATCCTGT GCCATCAAAA TCCAGTAACT GGATTGCTTC CAGCCAGCTA TGATCAGAAA
    GATGCCTGGG TCCGAGATAA TGTGTATAGT ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC
    TATCGGAAGA ATGCTGACCG GGATGAGGAT AAGGCAAAGG CCTATGAACT GGAGCAGAGT
    GTAGTGAAGC TGATGAGGAG CCTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAA
    TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CTAAGTACAA TACCAAAACC
    TGTGCCACTG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACCTCTTTG
    TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT
    GAAGTCAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC
    TTCGGGATAT GGGAACGTGG AGATAAGACT AACCAAGGGA TCTCAGAATT GAATGCCAGT
    TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTGGATG AACTGGACCT GTTTGGTGTG
    AAAGGTGGGC CCCAATCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT
    ATCCTTAATT CGATACTCCC CCGGGCTTCA ACATCCAAAG AGGTAGATGC TAGTCTGCTC
    TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAGCC AGTTGGTAGA GCTCACAAAG
    CAAGAAATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGCTTTCT ACGAGATGGA
    TATAAAACCC CTAAAGAGGA TCCCAGTCGC CTGTACTACG AACCATCTGA GTTGAAGTTA
    TTTGAAAATA TCGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGGTC
    TTTGGTGGCA ATGCAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGAAGC AGTCCTCATC
    AAGGGAAAAA ATGGAGTTCC ACTTTTGCCA GAGCTATACA GTGTTCCTCC AGACAAGGTT
    GATGAAGAAT ATCAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA GTTGCCTCAT
    ATGTGGGGTC AGTCTCTGTA CATCTTAGGA AGCTTGATGG CAGAGGGATT CTTAGCTCCT
    GGAGAAATTG ATCCCCTGAA TCGAAGGTTT TCTACAGTAC CAAAGCCAGA TGTTGTGGTT
    CAAGTTTCCA TTCTAGCCGA GACAGAAGAA ATCAAGGCCA TTTTGAAAGA CAAGGGAATC
    GATGTGGAGA CCATTGCTGA GGTATACCCT ATCAGAGTAC AACCAGCCTG TATTCTCAGC
    CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG
    CATATGGGAG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC
    ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACAA GATGATAGTA
    GAAATGCTTA GAACAGACCT CTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC
    ACCATCACCT TCCCCATCTC ACATACCATG CTTGATGATG ATGGAACCAG CTTGAATTCA
    AGTATCCTAG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGAGC AAGGATTCAA
    ACAGGTAAAT TGTCAGAGTT TTTAACAACA TCTTGCTGCA CACATTTGAG CTTCATGGAT
    CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTACGATG ATAACTATAA TGAGCTGGAA
    TCTGGCGACT GGATGAATGG CTATGGTTCA ACCAGTAATG CTCACTGTGG TGATGAAGTT
    GCTCGTTATT TAGATCACCT TTTGACGCAC ACTATTCCCC ATCCTAAACT GGCCCCTACC
    TCACAGAAGG GAGGGCTAGA TCGGTTTCAA GCTGCTGTGC AAACAACCTG CAATTTAATG
    TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT ATACAGAATG TTCACATGTA TCTTCCAACA
    AAGATATTTC AGGCTTCTCG GCCTTCGTTC AACTTACTTG ACTCACCTCA CTGCCCACGA
    GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACCA GTCTGGGGAG
    TTGGACTTCA AAGCACTGGT TTTACAGTTG AAGGAGACCT CCAGCTTACA GGAGCAAGCT
    GATATCCTTT ACATGCTGTA TACTATGAAA GGACCTGACT GGGACACTGA ATTATATGAT
    GAAGAAAGTG CAACAGTCCA AGAGCTGCTT ACTGAACTGT ATGGCAAAGT TGGAGAAATT
    CGTCACTGGG GCCTGATCCG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTT
    GATGAGGCCT GCACAGCCCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCTCCA
    GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTGCTCAC TCGGCTGATA
    GATGAGGCTA GTGAAGGGGA CATGAGCATC TCAATCCTTA CACAGGAAAT AATGGTATAT
    CTAGCCATGT ATATGCGAAC TCAGCCTGGC CTCTTCGCTG AAATGTTTCG ACTTCGAATT
    GGTCTAATCA TACAAGTTAT GGCAACAGAA CTGGCCCATT CCCTTCGATG CTCAGCTGAG
    GAAGCCACAG ACAGCCTAAT GAATCTTAGT CCTTCAGCCA TGAAGAACCT CCTGCATCAC
    ATTCTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCCACTGA TTCAAATGTT
    AGCCCTGCTA TTTCTATCCA TGAGATTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT
    GGAATAATGC AATTAAAAAG TGAGATAAAG CAGGTGGAAT TTCGTAGACT ATCTGTCTCA
    ACTGAGAGTC AGTCCAATGG TGGTCATCCC TTGGGTATAG ATTTGATGTC ACCGTCTTTT
    CTGCCCATAA AAGCAACAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.