plekhm2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plekhm2
Entrez Gene ID	564823
Full Name	pleckstrin homology domain containing, family M (with RUN domain) member 2
Synonyms	zgc:194971
General protein information	Preferred Names pleckstrin homology domain containing, family M (with RUN domain) member 2 Names pleckstrin homology domain-containing family M member 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296853.3	XP_009295128.1	pleckstrin homology domain-containing family M member 2 isoform X1
XM_009296853.2	XP_009295128.1	pleckstrin homology domain-containing family M member 2 isoform X1
NM_001130783.1	NP_001124255.1	pleckstrin homology domain-containing family M member 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLEKHM2	XP_417616.3
Pan troglodytes (chimpanzee)	PLEKHM2	XP_513054.3
Homo sapiens (human)	PLEKHM2	NP_055979.2
Bos taurus (cattle)	PLEKHM2	NP_001179345.1
Mus musculus (house mouse)	Plekhm2	NP_001028322.1
Rattus norvegicus (Norway rat)	Plekhm2	NP_001178696.1
	PLEKHM2	XP_001089676.1
Canis lupus familiaris (dog)	PLEKHM2	XP_005618020.1
Danio rerio (zebrafish)	plekhm2	NP_001124255.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following plekhm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plekhm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33150	NM_001130783.1 Latest version!	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27714	XM_009296853.3 Latest version!	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa27714	XM_009296853.2	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plekhm2 ( NM_001130783.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124255.1 Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), mRNA.

Price & Availability↑

CloneID	ODa33150
Clone ID Related Accession (Same CDS sequence)	NM_001130783.1
Accession Version	NM_001130783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family M member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163454.1. On Aug 6, 2008 this sequence version replaced XM_688153.3. ##Evidence-Data-START## Transcript exon combination :: BC163454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG ATTATGATTT TGGTGACATC 
TTCCCTGTGT TGCAGTCATT GCCAAGTGCA GACTGGGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCGTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTGTA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTAAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTC AAACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAGAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAAAATGGC 
TGTAGGGAAC CTCCGTACCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCTAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAC 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACACCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCCGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAATTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAGA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGT 
GACAGTTTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124255.1
CDS	21..3005
Translation	MDQLKVKDRILENISLSVKKLQSYFAACEDETPAIRNHDRVLQRLCEHLDHALLYGLQDISSGYWVLVLHFTRREAIRQIEELQHIATNLGRSRAWLYLALNESSLESYLRLFEENQGLLQKYYYKNALVCSHDHLTLFLTLVSGLEFIRFNLELDVPYLDVAPYMPEYYKPQNLLDFEERLPSSDSLSLHSFTSLTSTNLEWDDSAIAPSSEDYDFGDIFPVLQSLPSADWEEGDLTDPVSGPRSSGSDPQTVVSDCVVVKASAGTIRTTSLSRSPTFRQNPFTEDSDTNTSADVTPVHIASRHTSTTGEDAEITSTELEVIRMARRRKPGKKRRGRGSSVSSEQNVISPEVTDECLQIVVERDVTVNHITVDSRVPLERADSTASEPLRNGRREGEEEEPETGLRLPEMTDTSMDNVGQPLREVMDRLNGSLDAEDGAEEICTAHDGPPNQQPFREDAGGEPPDPSDGFLQAPLPPADFYCFTSQSPDPAASGGGRHDSAGHGEPQDVPVSNEDEGEAEAEAAAAGQDPSIAIETSEKKLSQTEEDDEDATQSSHAAEFKVDNNHLLLLMIHVFRENEEQLFKMVRMSTGHMEGDLQPLYLLLTDCYIYLLRKGAAEKPYSVEEAVSYNELDYISVGLHQQTITLVCTNRRRQFLLDTADSSLTDWFLTVLKEAWENGCREPPYPSILTDATMEKLALSKFVCQETHCEMSEMKIRLYSLVHWEDPMDVTVASPTSPSGSRASSSTKEGPLLYRAGSSYLGKEVWKSCYLVLSNGILYQYAERTDVTPLMSVTMGGGHCGGCRRSNSTEKPHTFQVILTEHQPLELSAENELEMADWMLLLCQSVSKGIIPQGVAPAPCIPCCLVLTDRKLLTCHQDCQTSFFRSLGGADLNDVTAVCLEDDKEYCVIEFAEDRAQFLPPWVLYFSGCKERDRLIAELSQAWTAVYQVSLPRKAVSDLSVQKKCSEALELMKSAWQRSDSLHRGRSEREPWC

Target ORF information:

RefSeq Version	NM_001130783.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130783.1

ORF Insert Sequence:

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG ATTATGATTT TGGTGACATC 
TTCCCTGTGT TGCAGTCATT GCCAAGTGCA GACTGGGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCGTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTGTA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTAAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTC AAACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAGAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAAAATGGC 
TGTAGGGAAC CTCCGTACCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCTAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAC 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACACCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCCGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAATTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAGA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGT 
GACAGTTTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhm2 ( XM_009296853.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295128.1 Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27714
Clone ID Related Accession (Same CDS sequence)	XM_009296853.3 , XM_009296853.2
Accession Version	XM_009296853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family M member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCTTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTATA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTGAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTG AGACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAAAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAGAATGGC 
TGTAGGGAAC CTCCATATCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCCAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAT 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACGCCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCTGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAGTTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAAA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGC 
GACAGTCTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295128.1
CDS	75..2999
Translation	MDQLKVKDRILENISLSVKKLQSYFAACEDETPAIRNHDRVLQRLCEHLDHALLYGLQDISSGYWVLVLHFTRREAIRQIEELQHIATNLGRSRAWLYLALNESSLESYLRLFEENQGLLQKYYYKNALVCSHDHLTLFLTLVSGLEFIRFNLELDVPYLDVAPYMPEYYKPQNLLDFEERLPSSDSLSLHSFTSLTSTNLEWDDSAIAPSSEEGDLTDPVSGPRSSGSDPQTVVSDCVIVKASAGTIRTTSLSRSPTFRQNPFTEDSDTNTSADVTPVHIASRHTSTTGEDAEITSTELEVIRMARRRKPGKKRRGRGSSVSSEQNVISPEVTDECLQIVVERDVTVNHITVDSRVPLERADSTASEPLRNGRREGEEEEPETGLRLPEMTDTSMDNVGQPLREVMDRLNGSLDAEDGAEEICTAHDGPPNQQPFREDAGGEPPDPSDGFLQAPLPPADFYCFTSQSPDPAASGGGRHDSAGHGEPQDVPVSNEDEGEAEAEAAAAGQDPSIAIETSEKKLSETEEDDEDATQSSHAAEFKVDNNHLLLLMIHVFRENEEQLFKMVRMSTGHMEGDLQPLYLLLTDCYIYLLRKGAAEKPYSVEEAVSYNELDYISVGLHQQTITLVCTNRRRQFLLDTADSSLTDWFLTVLKEAWENGCREPPYPSILTDATMEKLALSKFVCQETHCEMSEMKIRLYSLVHWEDPMDVTVASPTSPSGSRASSSTKEGPLLYRAGSSYLGKEVWKSCYLVLSNGILYQYAERTDVTPLMSVTMGGGHCGGCRRSNSTEKPHAFQVILTEHQPLELSAENELEMADWMLLLCQSVSKGIIPQGVAPAPCIPCCLVLTDRKLLTCHQDCQTSFFRSLGGADLNDVTAVCLEDDKEYCVIEFAEDRAQFLPPWVLYFSGCKERDRLIAELSQAWTAVYQVSLPRKAVSDLSVQKKCSEALELMKSAWQRSDSLHRGRSEREPWC

Target ORF information:

RefSeq Version	XM_009296853.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296853.3

ORF Insert Sequence:

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCTTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTATA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTGAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTG AGACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAAAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAGAATGGC 
TGTAGGGAAC CTCCATATCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCCAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAT 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACGCCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCTGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAGTTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAAA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGC 
GACAGTCTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhm2 ( XM_009296853.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295128.1 Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.

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CloneID	ODa27714
Clone ID Related Accession (Same CDS sequence)	XM_009296853.3 , XM_009296853.2
Accession Version	XM_009296853.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family M member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCTTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTATA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTGAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTG AGACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAAAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAGAATGGC 
TGTAGGGAAC CTCCATATCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCCAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAT 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACGCCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCTGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAGTTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAAA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGC 
GACAGTCTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295128.1
CDS	70..2994
Translation	MDQLKVKDRILENISLSVKKLQSYFAACEDETPAIRNHDRVLQRLCEHLDHALLYGLQDISSGYWVLVLHFTRREAIRQIEELQHIATNLGRSRAWLYLALNESSLESYLRLFEENQGLLQKYYYKNALVCSHDHLTLFLTLVSGLEFIRFNLELDVPYLDVAPYMPEYYKPQNLLDFEERLPSSDSLSLHSFTSLTSTNLEWDDSAIAPSSEEGDLTDPVSGPRSSGSDPQTVVSDCVIVKASAGTIRTTSLSRSPTFRQNPFTEDSDTNTSADVTPVHIASRHTSTTGEDAEITSTELEVIRMARRRKPGKKRRGRGSSVSSEQNVISPEVTDECLQIVVERDVTVNHITVDSRVPLERADSTASEPLRNGRREGEEEEPETGLRLPEMTDTSMDNVGQPLREVMDRLNGSLDAEDGAEEICTAHDGPPNQQPFREDAGGEPPDPSDGFLQAPLPPADFYCFTSQSPDPAASGGGRHDSAGHGEPQDVPVSNEDEGEAEAEAAAAGQDPSIAIETSEKKLSETEEDDEDATQSSHAAEFKVDNNHLLLLMIHVFRENEEQLFKMVRMSTGHMEGDLQPLYLLLTDCYIYLLRKGAAEKPYSVEEAVSYNELDYISVGLHQQTITLVCTNRRRQFLLDTADSSLTDWFLTVLKEAWENGCREPPYPSILTDATMEKLALSKFVCQETHCEMSEMKIRLYSLVHWEDPMDVTVASPTSPSGSRASSSTKEGPLLYRAGSSYLGKEVWKSCYLVLSNGILYQYAERTDVTPLMSVTMGGGHCGGCRRSNSTEKPHAFQVILTEHQPLELSAENELEMADWMLLLCQSVSKGIIPQGVAPAPCIPCCLVLTDRKLLTCHQDCQTSFFRSLGGADLNDVTAVCLEDDKEYCVIEFAEDRAQFLPPWVLYFSGCKERDRLIAELSQAWTAVYQVSLPRKAVSDLSVQKKCSEALELMKSAWQRSDSLHRGRSEREPWC

Target ORF information:

RefSeq Version	XM_009296853.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family M (with RUN domain) member 2 (plekhm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296853.2

ORF Insert Sequence:

ATGGATCAAC TCAAAGTCAA AGACAGGATC CTCGAAAACA TCTCTCTGTC CGTCAAGAAG 
TTGCAGAGTT ATTTTGCGGC CTGCGAGGAT GAGACGCCAG CCATCAGGAA TCACGACCGG 
GTTCTTCAGC GCTTGTGTGA ACACCTGGAT CACGCTCTGC TTTATGGGCT GCAGGACATT 
TCATCAGGTT ACTGGGTCCT CGTATTACAC TTCACACGAC GAGAAGCCAT TCGACAGATC 
GAGGAACTCC AACACATAGC CACCAATCTT GGCAGAAGCC GTGCCTGGCT GTACTTGGCG 
CTCAATGAAA GTTCTTTAGA AAGCTACCTC CGACTTTTTG AGGAGAATCA AGGACTGCTC 
CAGAAGTACT ATTACAAAAA TGCATTGGTT TGCAGTCATG ACCATCTCAC TCTGTTTCTT 
ACGCTCGTCT CTGGGCTTGA GTTCATCCGC TTTAACCTGG AGCTGGATGT TCCATACTTG 
GATGTTGCTC CCTACATGCC TGAATACTAC AAACCTCAGA ATCTGTTGGA TTTTGAGGAG 
AGGTTGCCGA GCTCTGATAG TCTGTCACTA CACTCGTTCA CTTCTCTCAC ATCCACTAAT 
CTCGAGTGGG ATGACAGTGC CATCGCCCCA TCCAGTGAAG AGGGAGACTT GACTGATCCA 
GTCAGTGGGC CGCGCTCTTC AGGATCAGAC CCTCAGACTG TTGTCAGTGA CTGTGTTATA 
GTAAAAGCCT CGGCGGGCAC CATCAGAACC ACATCTCTGT CAAGAAGCCC TACATTTCGG 
CAAAATCCCT TCACTGAGGA CTCGGACACC AACACTTCAG CTGACGTCAC CCCTGTGCAC 
ATAGCAAGCC GGCACACTTC AACAACTGGA GAAGACGCTG AAATCACCAG CACTGAGCTG 
GAAGTCATTA GAATGGCACG GCGACGAAAG CCTGGCAAGA AACGACGCGG CAGAGGATCA 
TCAGTGAGTA GTGAACAGAA CGTCATCTCT CCAGAAGTGA CTGATGAATG CCTTCAGATT 
GTAGTTGAGA GAGACGTCAC AGTAAACCAC ATCACCGTGG ACAGTCGAGT TCCTCTGGAG 
CGTGCTGACA GCACTGCTTC AGAACCCTTG AGGAATGGGA GACGCGAAGG AGAAGAAGAG 
GAGCCTGAAA CTGGACTGAG GCTTCCAGAA ATGACGGACA CTTCAATGGA CAACGTAGGG 
CAACCCCTCC GTGAGGTCAT GGACAGGCTG AATGGGTCTC TGGATGCTGA GGATGGAGCA 
GAAGAAATTT GCACCGCTCA CGACGGGCCC CCCAATCAGC AGCCCTTTCG AGAGGATGCT 
GGCGGTGAAC CTCCAGACCC AAGCGATGGT TTCCTGCAGG CCCCGCTGCC GCCTGCAGAC 
TTCTACTGCT TTACCTCCCA GAGTCCAGAC CCTGCTGCCT CAGGTGGTGG GCGCCATGAC 
TCTGCAGGGC ATGGCGAGCC GCAGGATGTT CCTGTTAGCA ATGAAGATGA GGGAGAAGCA 
GAAGCAGAGG CAGCAGCTGC AGGGCAGGAT CCCTCCATCG CCATAGAGAC CTCAGAGAAG 
AAACTGAGTG AGACAGAAGA AGATGATGAA GATGCAACAC AGAGCTCTCA TGCTGCAGAA 
TTCAAGGTGG ATAACAATCA TCTGCTGCTG CTCATGATTC ATGTTTTCAG GGAGAATGAA 
GAACAGCTCT TTAAGATGGT GAGGATGAGC ACAGGCCACA TGGAAGGAGA TCTGCAGCCT 
CTATATCTGC TCTTAACAGA CTGCTACATT TACCTCTTGC GAAAAGGAGC AGCAGAAAAA 
CCTTACAGTG TTGAGGAGGC AGTGTCTTAT AATGAGCTGG ACTACATCTC TGTGGGTCTT 
CACCAGCAGA CCATCACTCT GGTGTGCACC AACAGAAGAC GGCAGTTCCT GCTGGACACT 
GCGGACTCGT CTCTGACTGA CTGGTTTCTG ACTGTGTTGA AGGAGGCTTG GGAGAATGGC 
TGTAGGGAAC CTCCATATCC CTCTATTCTC ACTGACGCCA CCATGGAGAA ACTCGCTCTC 
TCCAAGTTTG TGTGCCAGGA GACTCACTGT GAGATGTCTG AAATGAAGAT TCGCCTTTAT 
TCTCTGGTTC ACTGGGAAGA CCCAATGGAT GTGACCGTGG CTTCACCGAC ATCTCCGTCG 
GGCTCTAGAG CATCCAGCAG CACTAAAGAG GGGCCGCTGC TGTACCGAGC CGGAAGCTCT 
TATTTGGGGA AAGAAGTGTG GAAAAGTTGT TACTTAGTTC TCAGCAATGG CATCCTGTAT 
CAGTATGCAG AGAGAACAGA TGTTACACCT TTGATGTCTG TTACCATGGG GGGAGGGCAT 
TGTGGAGGCT GCAGGCGTTC AAACAGCACA GAAAAACCTC ACGCCTTTCA GGTGATTCTG 
ACAGAGCATC AGCCATTAGA GCTCAGCGCA GAGAACGAGC TGGAAATGGC AGACTGGATG 
CTGCTGCTCT GCCAGTCTGT CTCTAAAGGG ATTATCCCAC AGGGAGTTGC TCCTGCACCC 
TGCATCCCAT GTTGCCTTGT GCTCACAGAC AGGAAGTTAC TCACGTGTCA TCAGGATTGC 
CAGACCAGTT TTTTCCGTTC TCTTGGAGGA GCCGATTTGA ACGATGTCAC AGCTGTTTGC 
CTGGAGGACG ACAAGGAATA CTGTGTCATT GAGTTTGCAG AGGATCGAGC TCAGTTTCTT 
CCTCCCTGGG TTTTGTATTT TAGCGGATGT AAGGAACGAG ATCGGCTTAT AGCAGAATTA 
AGTCAAGCAT GGACTGCTGT ATATCAGGTG AGTCTACCTC GTAAGGCAGT GTCAGACCTG 
TCGGTTCAAA AGAAGTGCAG CGAGGCTCTG GAGCTGATGA AGAGCGCCTG GCAGAGAAGC 
GACAGTCTAC ACAGAGGCCG ATCAGAAAGA GAACCATGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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plekhm2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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