PLEKHM2 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHM2
Entrez Gene ID	515552
Full Name	pleckstrin homology and RUN domain containing M2
General protein information	Preferred Names pleckstrin homology and RUN domain containing M2 Names pleckstrin homology domain-containing family M member 2 pleckstrin homology domain containing, family M (with RUN domain) member 2
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976216.1	XP_024831984.1	pleckstrin homology domain-containing family M member 2 isoform X2
NM_001192416.2	NP_001179345.1	pleckstrin homology domain-containing family M member 2
XM_024976215.1	XP_024831983.1	pleckstrin homology domain-containing family M member 2 isoform X1
XM_024976214.1	XP_024831982.1	pleckstrin homology domain-containing family M member 2 isoform X1
NM_001192416.1	NP_001179345.1	pleckstrin homology domain-containing family M member 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLEKHM2	XP_417616.3
Pan troglodytes (chimpanzee)	PLEKHM2	XP_513054.3
Homo sapiens (human)	PLEKHM2	NP_055979.2
Bos taurus (cattle)	PLEKHM2	NP_001179345.1
Mus musculus (house mouse)	Plekhm2	NP_001028322.1
Rattus norvegicus (Norway rat)	Plekhm2	NP_001178696.1
	PLEKHM2	XP_001089676.1
Canis lupus familiaris (dog)	PLEKHM2	XP_005618020.1
Danio rerio (zebrafish)	plekhm2	NP_001124255.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLEKHM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223556	XM_024976215.1 Latest version!	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa223556	XM_024976214.1 Latest version!	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa223557	XM_024976216.1 Latest version!	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa216257	NM_001192416.2 Latest version!	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa01788	NM_001192416.1 Latest version!	Bos taurus pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHM2 ( XM_024976215.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024831983.1 Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223556
Clone ID Related Accession (Same CDS sequence)	XM_024976215.1 , XM_024976214.1
Accession Version	XM_024976215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology domain-containing family M member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCCC TGCCATCCGC AACCACGACA AAGTCCTGCA GCGTCTATGT GAGCACTTGG 
ACCACGCTCT GCTCTACGGG CCGGGCCTGG CTGTACCTGG CCCTCAATGA AAACTCCCTA 
GAGAGCTACC TGCGGCTGTT CCAGGAGAAC CTGGGTCTCT TGCACAAGTA CTATGTCAAG 
AATGCCCTGG TGTGTAGCCA TGACCACCTG ACCCTCTTCC TGACCTTGGT ATCCGGGCTG 
GAGTTCATTC GGTTTGACCT GGACCTGGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831983.1
CDS	139..3099
Translation	MRPLPSATTTKSCSVYVSTWTTLCSTGRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDPVVSSPGPGQGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQLNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYRFTVESPSAVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDAQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSPALCLAMAAPVAAAAKLRVHVGCGFRQVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	XM_024976215.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976215.1

ORF Insert Sequence:

ATGAGACCCC TGCCATCCGC AACCACGACA AAGTCCTGCA GCGTCTATGT GAGCACTTGG 
ACCACGCTCT GCTCTACGGG CCGGGCCTGG CTGTACCTGG CCCTCAATGA AAACTCCCTA 
GAGAGCTACC TGCGGCTGTT CCAGGAGAAC CTGGGTCTCT TGCACAAGTA CTATGTCAAG 
AATGCCCTGG TGTGTAGCCA TGACCACCTG ACCCTCTTCC TGACCTTGGT ATCCGGGCTG 
GAGTTCATTC GGTTTGACCT GGACCTGGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHM2 ( XM_024976214.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024831982.1 Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223556
Clone ID Related Accession (Same CDS sequence)	XM_024976215.1 , XM_024976214.1
Accession Version	XM_024976214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology domain-containing family M member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCCC TGCCATCCGC AACCACGACA AAGTCCTGCA GCGTCTATGT GAGCACTTGG 
ACCACGCTCT GCTCTACGGG CCGGGCCTGG CTGTACCTGG CCCTCAATGA AAACTCCCTA 
GAGAGCTACC TGCGGCTGTT CCAGGAGAAC CTGGGTCTCT TGCACAAGTA CTATGTCAAG 
AATGCCCTGG TGTGTAGCCA TGACCACCTG ACCCTCTTCC TGACCTTGGT ATCCGGGCTG 
GAGTTCATTC GGTTTGACCT GGACCTGGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831982.1
CDS	154..3114
Translation	MRPLPSATTTKSCSVYVSTWTTLCSTGRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDPVVSSPGPGQGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQLNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYRFTVESPSAVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDAQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSPALCLAMAAPVAAAAKLRVHVGCGFRQVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	XM_024976214.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976214.1

ORF Insert Sequence:

ATGAGACCCC TGCCATCCGC AACCACGACA AAGTCCTGCA GCGTCTATGT GAGCACTTGG 
ACCACGCTCT GCTCTACGGG CCGGGCCTGG CTGTACCTGG CCCTCAATGA AAACTCCCTA 
GAGAGCTACC TGCGGCTGTT CCAGGAGAAC CTGGGTCTCT TGCACAAGTA CTATGTCAAG 
AATGCCCTGG TGTGTAGCCA TGACCACCTG ACCCTCTTCC TGACCTTGGT ATCCGGGCTG 
GAGTTCATTC GGTTTGACCT GGACCTGGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHM2 ( XM_024976216.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024831984.1 Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X3, mRNA.

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CloneID	OBa223557
Clone ID Related Accession (Same CDS sequence)	XM_024976216.1
Accession Version	XM_024976216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology domain-containing family M member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGTC ATGGTCACGG TCACGCACTA GAGCCATTGC AGTGCAGCCC AGGAGCTCCA 
GAGGCACCTC TGCAGCCTCG TCAAGATGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831984.1
CDS	103..2883
Translation	MVSHGHGHALEPLQCSPGAPEAPLQPRQDDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDPVVSSPGPGQGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQLNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYRFTVESPSAVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDAQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSPALCLAMAAPVAAAAKLRVHVGCGFRQVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	XM_024976216.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976216.1

ORF Insert Sequence:

ATGGTCAGTC ATGGTCACGG TCACGCACTA GAGCCATTGC AGTGCAGCCC AGGAGCTCCA 
GAGGCACCTC TGCAGCCTCG TCAAGATGAT GCCCCTTATT TAGACCTGGC CCCCTACATG 
CCCGACTACT ACAAGCCGCA GTACCTGCTG GACTTTGAAG ACCGCCTCCC CAGCTCAGTC 
CACGGCTCAG ACAGCCTCTC CCTCAACTCC TTCAACTCCG TCACCTCCAC CACCCTGGAG 
TGGGATGACA GCGCCATCGC CCCGTCTAGT GAGGATTATG ATTTTGGAGA TGTGTTTCCA 
GCAGTGCCGT CCGTACCCAG CACAGACTGG GAAGACGGAG ACCTCACAGA CACTGTCAGC 
GGCCCCCGCT CCACCACCTC AGACCCAACC AGCAGCAAGG CTTCCACCAA GAGTCCCACC 
CAGCGCCACA ACCCCTTCAA TGAGGACCAG GCCGAGACTG TGTCCTCCTC CGACACCACC 
CCTGTGCACA CAAGCTCTCA GGAGAAGGGG GAGTCTCACA CTCCAGACCT ACCGGACACC 
TGCACAGAGC TCGAGGTCAT CAGGGTCACC AAGAAGAAGA AAACAGGCAA GAAGAAGAAG 
CCCAGATCAG ATGAGGAAGC AAGTCCACTC CACCCAGCCT CCACCCAGCA CACGTGTGCC 
CGGCAGGGCA ATGGCGACCC CGTGGTCAGC AGCCCAGGCC CTGGCCAGGG CTCCCCAGAC 
ACCACCCTGG CCTCCCCGCC ACAGGAGGGA CAGGGGCCTG GCAGCACAGT GGAGGGCAGC 
GAGCGCTCAG AGCCCGGCCA GGTGGGCCTG CTTATCCCCG AGATGAAGGA CACCTCCATG 
GAGCGCGTGG GGCAGCCCCT GAGTAAGGTC ATCGACCAGC TGAACGGGCA GCTGGACCCG 
GCCACCTGGC GCTCCCACGT CGAGCCCCCG GACCAGTCCT TTCGGACCGG CTCTCCCGGG 
GACGCCCCGG AGAGGCCGCC ATTTTGCGAC TTTAGTGAGG GGCTTCCAGC CCCAATGGAC 
TTCTACCGCT TTACCGTCGA GAGTCCAAGT GCTGTTACAT CAGGTGGCGG CCACCATGAC 
CCTGCAGGGC CTGGCCAACC GCTGCATGTT CCTGGTAGCC CTGCGGCTGC TGGCCAAGAA 
GAGGCGGGGG GAGGAGGAGG ACCAGGACAG ACGCCTCGGC CCTTAGAGGA CACCCCGGGG 
GAGGCACAGA AGCCAGAGGC CCAGGAACTG GACGCCCAGC TGCCCCAGGA CGGTGAGGGG 
CTGGAGCCAG AGCCTGGGAC CCAGGAGGCT CTTTGCAGAC TCAAGGGAGA CCAGCCCAGC 
CCCTGTCTGA GCAGCGCCGA GGACTCTGGG GTGGAGGAGG GCCAGGGGAG CCCTTCTGAG 
ATGACCCACT CGGCAGAGTT CAGAGTAGAC AACAATCACC TGCTCCTGCT GATGATCCAT 
GTGTTTCGAG AAAACGAAGA GCAGCTCTTC AGAATGATCC GGATGAGCAC CGGGCACATG 
GAGGGCAACC TGCAGCTGCT GTACGTGCTG CTCACAGACT GCTATGTCTA CCTGCTCCGG 
AAAGGGGCCA CAGAGAAGCC ATACTTGGTG GAAGAGGCTG TTTCTTACAA TGAGCTCGAC 
TACGTGTCGC CCGCCCTCTG TCTGGCCATG GCTGCCCCTG TGGCAGCCGC GGCCAAGCTC 
CGTGTGCATG TGGGGTGTGG TTTCCGGCAG GTGGGCCTCG ACCAACAGAC GGTGAAGCTG 
GTGTGCACCA ACCGCAGGAA GCAGTTTCTG CTGGACACAG CGGACGTGGC CCTGGCAGAG 
TTCTTCTTGG CTTCTTTGAA GTCAGCCATG ATCAAGGGCT GTCGGGAACC CCCCTACCCC 
AGCATCCTGA CTGATGCCAC CATGGAGAAG CTGGCTCTGG CCAAATTTGT GGCCCAGGAA 
TCTAAGTGTG AGGCGACTGC TGTTACTGTG CGCTTCTATG GGCTTGTGCA CTGGGAGGAC 
CCCCAGGACG AGTTCCTGGG CCCCACCCCC TGCCACTGCT CACCCCCCGA GGGCACCATC 
ACCAAAGAAG GCATGCTGCA TTACAAGGCA GGCACCTCCT ACCTGGGCAA GGAGCACTGG 
AAGACTTGCT TCGTGGTGCT CAGCAACGGG ATCCTCTACC AATATCCTGA CCGCACCGAC 
GTGACGCCCC TGCTCTCAGT GAACATGGGG GGGGAGCAGT GCGGTGGCTG TCGGAGATCC 
AACACCACGG ATCGGCCCCA CGCCTTCCAG GTCATCCTCG CCGACCGGCC CTGCCTTGAG 
CTGAGCGCCG ACAGCGAGGC TGAGATGGCC GACTGGATGC AGCACCTGTG CCAGGCCGTG 
TCCAAGGGGG TCATCCCCCA GGGGGTAGCT CCCAGCCCTT GCATCCCTTG CTGCCTGGTA 
ATCACCGATG ACCGCCTCTT CACGTGCCAC GAGGATTGCC AGACCAGCTT CTTCCGCTCC 
CTGGGTACTG CCAAGCTGGC TGACATCAGC GCTGTGTCCA CTGAACCGGG CAAGGAGTAC 
TGCCTCTTGG AGTTCTCCCA GGACAGCGCG CAGCCCCTCC CACCCTGGGT CGTCTACCTG 
AGCTGCACAC CTGAACTGGA CCGATTCCTA TCTGCACTGA ACTCGGGGTG GAAAACCATC 
TACCAGGTGG ACCTCCCCCA CAAGGCCATC CAGGAAGCCT CCAAGAAGAA GTTCGAGGAT 
GCCCTGAGCC TCATCCACAG TGCCTGGCAG CGGAGCGACA GCCTGTGCCG GGGCAGAGCC 
TCCCGGGACC CCTGGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHM2 ( NM_001192416.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179345.1 Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.

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CloneID	OBa216257
Clone ID Related Accession (Same CDS sequence)	NM_001192416.2
Accession Version	NM_001192416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	pleckstrin homology domain-containing family M member 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB438536.1, DN538966.1 and NKLS02000016.1. On Aug 24, 2017 this sequence version replaced NM_001192416.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571257.47784.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTATGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAATGAAA ACTCCCTAGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTCTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCGCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTCCA CGGCTCAGAC AGCCTCTCCC TCAACTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCC GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGCGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTG 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCTCACACT 
CCAGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACCCCG TGGTCAGCAG CCCAGGCCCT 
GGCCAGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGCTG 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACCGCTTT ACCGTCGAGA GTCCAAGTGC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCACAGAAG CCAGAGGCCC AGGAACTGGA CGCCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCTGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ACGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCAGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCCA AATTTGTGGC CCAGGAATCT 
AAGTGTGAGG CGACTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCCGAGGG CACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCAGGC ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACTTGCTTCG TGGTGCTCAG CAACGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCAGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTTGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC GTGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCTGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCGCAG CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCACAA GGCCATCCAG GAAGCCTCCA AGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179345.1
CDS	28..3105
Translation	MEPREVKDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAVKHIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDPVVSSPGPGQGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQLNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYRFTVESPSAVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDAQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	NM_001192416.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RUN domain containing M2 (PLEKHM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192416.2

ORF Insert Sequence:

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTATGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAATGAAA ACTCCCTAGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTCTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCGCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTCCA CGGCTCAGAC AGCCTCTCCC TCAACTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCC GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGCGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTG 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCTCACACT 
CCAGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACCCCG TGGTCAGCAG CCCAGGCCCT 
GGCCAGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGCTG 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACCGCTTT ACCGTCGAGA GTCCAAGTGC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCACAGAAG CCAGAGGCCC AGGAACTGGA CGCCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCTGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ACGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCAGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCCA AATTTGTGGC CCAGGAATCT 
AAGTGTGAGG CGACTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCCGAGGG CACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCAGGC ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACTTGCTTCG TGGTGCTCAG CAACGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCAGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTTGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC GTGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCTGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCGCAG CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCACAA GGCCATCCAG GAAGCCTCCA AGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHM2 ( NM_001192416.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179345.1 Bos taurus pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.

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CloneID	OBa01788
Clone ID Related Accession (Same CDS sequence)	NM_001192416.1
Accession Version	NM_001192416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-15
Organism	Bos taurus(Cattle)
Product	pleckstrin homology domain-containing family M member 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03088645.1. On Jul 17, 2010 this sequence version replaced XM_593587.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTATGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAATGAAA ACTCCCTAGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTCTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCGCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTCCA CGGCTCAGAC AGCCTCTCCC TCAACTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCC GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGCGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTG 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCTCACACT 
CCAGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACCCCG TGGTCAGCAG CCCAGGCCCT 
GGCCAGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGCTG 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACCGCTTT ACCGTCGAGA GTCCAAGTGC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCACAGAAG CCAGAGGCCC AGGAACTGGA CGCCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCTGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ACGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCAGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCCA AATTTGTGGC CCAGGAATCT 
AAGTGTGAGG CGACTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCCGAGGG CACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCAGGC ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACTTGCTTCG TGGTGCTCAG CAACGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCAGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTTGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC GTGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCTGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCGCAA CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCACAA GGCCATCCAG GAAGCCTCCA AGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179345.1
CDS	8..3085
Translation	MEPREVKDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAVKHIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTTLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTTSDPTSSKASTKSPTQRHNPFNEDQAETVSSSDTTPVHTSSQEKGESHTPDLPDTCTELEVIRVTKKKKTGKKKKPRSDEEASPLHPASTQHTCARQGNGDPVVSSPGPGQGSPDTTLASPPQEGQGPGSTVEGSERSEPGQVGLLIPEMKDTSMERVGQPLSKVIDQLNGQLDPATWRSHVEPPDQSFRTGSPGDAPERPPFCDFSEGLPAPMDFYRFTVESPSAVTSGGGHHDPAGPGQPLHVPGSPAAAGQEEAGGGGGPGQTPRPLEDTPGEAQKPEAQELDAQLPQDGEGLEPEPGTQEALCRLKGDQPSPCLSSAEDSGVEEGQGSPSEMTHSAEFRVDNNHLLLLMIHVFRENEEQLFRMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEATAVTVRFYGLVHWEDPQDEFLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVTPLLSVNMGGEQCGGCRRSNTTDRPHAFQVILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGVAPSPCIPCCLVITDDRLFTCHEDCQTSFFRSLGTAKLADISAVSTEPGKEYCLLEFSQDSAQPLPPWVVYLSCTPELDRFLSALNSGWKTIYQVDLPHKAIQEASKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC

Target ORF information:

RefSeq Version	NM_001192416.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192416.1

ORF Insert Sequence:

ATGGAGCCGC GGGAGGTGAA GGACCGGATT CTGGAGAACA TCTCGCTGTC GGTGAAGAAG 
TTGCAGAGCT ACTTTGCTGC CTGTGAAGAT GAGACCCCTG CCATCCGCAA CCACGACAAA 
GTCCTGCAGC GTCTATGTGA GCACTTGGAC CACGCTCTGC TCTACGGGCT GCAGGACCTC 
TCATCTGGTT ACTGGGTGCT CGTGGTTCAT TTCACCAGGA GAGAGGCCGT CAAGCACATC 
GAGGTGCTAC AGCACGTGGC CACCAACCTG GGGCGCAGCC GGGCCTGGCT GTACCTGGCC 
CTCAATGAAA ACTCCCTAGA GAGCTACCTG CGGCTGTTCC AGGAGAACCT GGGTCTCTTG 
CACAAGTACT ATGTCAAGAA TGCCCTGGTG TGTAGCCATG ACCACCTGAC CCTCTTCCTG 
ACCTTGGTAT CCGGGCTGGA GTTCATTCGG TTTGACCTGG ACCTGGATGC CCCTTATTTA 
GACCTGGCCC CCTACATGCC CGACTACTAC AAGCCGCAGT ACCTGCTGGA CTTTGAAGAC 
CGCCTCCCCA GCTCAGTCCA CGGCTCAGAC AGCCTCTCCC TCAACTCCTT CAACTCCGTC 
ACCTCCACCA CCCTGGAGTG GGATGACAGC GCCATCGCCC CGTCTAGTGA GGATTATGAT 
TTTGGAGATG TGTTTCCAGC AGTGCCGTCC GTACCCAGCA CAGACTGGGA AGACGGAGAC 
CTCACAGACA CTGTCAGCGG CCCCCGCTCC ACCACCTCAG ACCCAACCAG CAGCAAGGCT 
TCCACCAAGA GTCCCACCCA GCGCCACAAC CCCTTCAATG AGGACCAGGC CGAGACTGTG 
TCCTCCTCCG ACACCACCCC TGTGCACACA AGCTCTCAGG AGAAGGGGGA GTCTCACACT 
CCAGACCTAC CGGACACCTG CACAGAGCTC GAGGTCATCA GGGTCACCAA GAAGAAGAAA 
ACAGGCAAGA AGAAGAAGCC CAGATCAGAT GAGGAAGCAA GTCCACTCCA CCCAGCCTCC 
ACCCAGCACA CGTGTGCCCG GCAGGGCAAT GGCGACCCCG TGGTCAGCAG CCCAGGCCCT 
GGCCAGGGCT CCCCAGACAC CACCCTGGCC TCCCCGCCAC AGGAGGGACA GGGGCCTGGC 
AGCACAGTGG AGGGCAGCGA GCGCTCAGAG CCCGGCCAGG TGGGCCTGCT TATCCCCGAG 
ATGAAGGACA CCTCCATGGA GCGCGTGGGG CAGCCCCTGA GTAAGGTCAT CGACCAGCTG 
AACGGGCAGC TGGACCCGGC CACCTGGCGC TCCCACGTCG AGCCCCCGGA CCAGTCCTTT 
CGGACCGGCT CTCCCGGGGA CGCCCCGGAG AGGCCGCCAT TTTGCGACTT TAGTGAGGGG 
CTTCCAGCCC CAATGGACTT CTACCGCTTT ACCGTCGAGA GTCCAAGTGC TGTTACATCA 
GGTGGCGGCC ACCATGACCC TGCAGGGCCT GGCCAACCGC TGCATGTTCC TGGTAGCCCT 
GCGGCTGCTG GCCAAGAAGA GGCGGGGGGA GGAGGAGGAC CAGGACAGAC GCCTCGGCCC 
TTAGAGGACA CCCCGGGGGA GGCACAGAAG CCAGAGGCCC AGGAACTGGA CGCCCAGCTG 
CCCCAGGACG GTGAGGGGCT GGAGCCAGAG CCTGGGACCC AGGAGGCTCT TTGCAGACTC 
AAGGGAGACC AGCCCAGCCC CTGTCTGAGC AGCGCCGAGG ACTCTGGGGT GGAGGAGGGC 
CAGGGGAGCC CTTCTGAGAT GACCCACTCG GCAGAGTTCA GAGTAGACAA CAATCACCTG 
CTCCTGCTGA TGATCCATGT GTTTCGAGAA AACGAAGAGC AGCTCTTCAG AATGATCCGG 
ATGAGCACCG GGCACATGGA GGGCAACCTG CAGCTGCTGT ACGTGCTGCT CACAGACTGC 
TATGTCTACC TGCTCCGGAA AGGGGCCACA GAGAAGCCAT ACTTGGTGGA AGAGGCTGTT 
TCTTACAATG AGCTCGACTA CGTGTCGGTG GGCCTCGACC AACAGACGGT GAAGCTGGTG 
TGCACCAACC GCAGGAAGCA GTTTCTGCTG GACACAGCGG ACGTGGCCCT GGCAGAGTTC 
TTCTTGGCTT CTTTGAAGTC AGCCATGATC AAGGGCTGTC GGGAACCCCC CTACCCCAGC 
ATCCTGACTG ATGCCACCAT GGAGAAGCTG GCTCTGGCCA AATTTGTGGC CCAGGAATCT 
AAGTGTGAGG CGACTGCTGT TACTGTGCGC TTCTATGGGC TTGTGCACTG GGAGGACCCC 
CAGGACGAGT TCCTGGGCCC CACCCCCTGC CACTGCTCAC CCCCCGAGGG CACCATCACC 
AAAGAAGGCA TGCTGCATTA CAAGGCAGGC ACCTCCTACC TGGGCAAGGA GCACTGGAAG 
ACTTGCTTCG TGGTGCTCAG CAACGGGATC CTCTACCAAT ATCCTGACCG CACCGACGTG 
ACGCCCCTGC TCTCAGTGAA CATGGGGGGG GAGCAGTGCG GTGGCTGTCG GAGATCCAAC 
ACCACGGATC GGCCCCACGC CTTCCAGGTC ATCCTCGCCG ACCGGCCCTG CCTTGAGCTG 
AGCGCCGACA GCGAGGCTGA GATGGCCGAC TGGATGCAGC ACCTGTGCCA GGCCGTGTCC 
AAGGGGGTCA TCCCCCAGGG GGTAGCTCCC AGCCCTTGCA TCCCTTGCTG CCTGGTAATC 
ACCGATGACC GCCTCTTCAC GTGCCACGAG GATTGCCAGA CCAGCTTCTT CCGCTCCCTG 
GGTACTGCCA AGCTGGCTGA CATCAGCGCT GTGTCCACTG AACCGGGCAA GGAGTACTGC 
CTCTTGGAGT TCTCCCAGGA CAGCGCGCAA CCCCTCCCAC CCTGGGTCGT CTACCTGAGC 
TGCACACCTG AACTGGACCG ATTCCTATCT GCACTGAACT CGGGGTGGAA AACCATCTAC 
CAGGTGGACC TCCCCCACAA GGCCATCCAG GAAGCCTCCA AGAAGAAGTT CGAGGATGCC 
CTGAGCCTCA TCCACAGTGC CTGGCAGCGG AGCGACAGCC TGTGCCGGGG CAGAGCCTCC 
CGGGACCCCT GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PLEKHM2 cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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