ARHGEF12A cDNA ORF clone, Danio rerio(Zebrafish)

The following ARHGEF12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa43817 XM_009291731.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa43818 XM_009291732.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa43819 XM_009291733.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa43819 XM_009291733.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa43817 XM_009291731.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa43818 XM_009291732.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa43817
    Clone ID Related Accession (Same CDS sequence) XM_009291731.3 , XM_009291731.2
    Accession Version XM_009291731.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGACC TCCTGATGTG GGCACACCTA AACCGAGCAG AAGGTTTGAG
    GATCTGCAGA GTGAGGATGA TCAGGGGAGT GATGTTTACT CTGATGTAGA TCCTCCAAAC
    TGGCAGCAGC TGGTCGGGCG TGATGTTTTA GCAGGCCTGA CGCCCCACGA GATCAAGAGA
    CAAGAAGTCA TTAATGAGCT GTTCTACACG GAGCGAGCTC ACGTACGCAT GTTGAAGGTC
    CTAGACAAGG TCTTCTACCA GAAACTCAAC CGAGACAACA TACTGCCCCC TGCTGACATC
    AAGTACATAT TCACCAACCT GGAGGAAATC ATCCAACTCC ACGTGAGAAT AGTGGATCAA
    ATGACAGCAA TTTGCAAGAA GAACGAGACG TCTGTAATCG ACCACATAGG AGACGAGTTG
    TTGTCTTGGT TCAGCAGTGG AGAGGAGGAG AAGATCAAGC AAGCAGTGGG CACTTTCTGC
    AGTAACCAGC CCTTCGCCCT GGAGCTCATC AAATCCCGAC AGAAGAAGGA CCAACGTTTC
    TCCTCCTTCA TGCAGGAGGC TGAAAGCAAT CGACTGTGTC GGCGTCTACA GCTAAAGGAC
    ATCATCCCTG CAGAGATGCA GAGGTTCACC AAATACCCAT TATTAATGGA CAACATTGCC
    AAATACACAG ATGAGTCAGA GGAAAAAGAC AAAGTGAGAC GAGCAGCAGA ATGCTGTCGA
    CAGATTCTCA ACCACGTCAA TCAGTCTGTG AAGGAGTCCG AGAATAAACA GAGACTGGAA
    GACTACCAGA GACGGCTGGA CCTCTCTTCC CTCAAGCAGA GCGAGAACCC AATGATCTCA
    GAGTTCAAGA ACCTGGATCT CACTAAACGA AAGATGGTCC ACGAGGGTCC TCTCTCCTGG
    AAGATAAATA AAGACAAAAC AATTGAGTTG TATACGTTAC TCCTGGAAGA CATACTGGTG
    CTGCTGCAGA AGCAGGACGA GCGTCTCATT CTCAGGTGTC ACAGTAAGAA CCTGGCAGGA
    ACAGCTGAAA CCAAACACAT CTTCAGCCCT ATCATCAAGC TCAGCACTGT GCTGGTGCGC
    TCTGTGGCCA CAGATAATAG ATCATTCTTT GTGATCTCCA TGTCCAAAAA CGGAGCTCAG
    ATTTATGAGC TGATGGCCCA GACGGTGTCT GAGCAGAGGA TGTGGCAACG GCAGATCACT
    CAGAGGGCAG AGGTTATGAA CACCAAACCT CACAGTGTCA TTCCTTTGCC TCAACACGAT
    GGCGACAGAG ACGGTGTGGA GTTAAGCAAG TTCAGCAAAG GCTCTGATCG GATCTCCACA
    GGAAGCATCC AGTCCGCAGA GAAAGACGGT GAATTTTCGC CCTCAGCAGC CCTGCGGGAC
    TGTGTGCCCG ATACCAACCC TGGGGAAGAG GACCAGGTCG CGAGAGCTGG CAGAGACGCT
    TCCTATCAGG ACTCTGAACT TTTGAACGGC AGGGAAACAG ACTCTCTATC TTGTTCATCT
    AAAGCTGACG AGGCCCTGAA AACATTGGCA GCGCTAAAGC AGGTGCTGCT GACTCAGCTG
    ATGGCTCAGG ATGAGGGTGA GCGATTGGGC CGATCGGCCG GCAGTCGTCT GCTCAGGACC
    ACCTCTTTAC GCACACCAGT GGAGCACAAC TCCAACCGCA CCCCTAGTCG AGCTCAGGAC
    TACACCTCTG GCGACACAGG CTTCTATGAG TGCCCGGAGG ATTACGCAGC GTACCTGGTT
    CTGGAGGGCT TCGGTGGCTC GGGCGAGAGC AGCACTGATG ATGATTTTCA GGTTGGGAGA
    AAGGAGCCTG GTTTGGCCCA CAGCTGCGCT GCTGACTCTG GGATCAGCCT GAAGTTCTCC
    TCGCCCTCCT CTGGCAGCAC GTCCCGTCTC AGCAGACAGG TGCTCTCACA CCTGCGCTGC
    CTGCAGGCCA ACCTCAACCA CCTCAAGGAG ATGGAAACTA AGTATTATAA TCTACTTCGC
    CAAAGGCCTG TCGGATCAGC AGCAGAAGCA GACGAGAGCA GAGAGAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290006.1
    CDS903..4976
    Translation

    Target ORF information:

    RefSeq Version XM_009291731.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291731.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGACC TCCTGATGTG GGCACACCTA AACCGAGCAG AAGGTTTGAG
    GATCTGCAGA GTGAGGATGA TCAGGGGAGT GATGTTTACT CTGATGTAGA TCCTCCAAAC
    TGGCAGCAGC TGGTCGGGCG TGATGTTTTA GCAGGCCTGA CGCCCCACGA GATCAAGAGA
    CAAGAAGTCA TTAATGAGCT GTTCTACACG GAGCGAGCTC ACGTACGCAT GTTGAAGGTC
    CTAGACAAGG TCTTCTACCA GAAACTCAAC CGAGACAACA TACTGCCCCC TGCTGACATC
    AAGTACATAT TCACCAACCT GGAGGAAATC ATCCAACTCC ACGTGAGAAT AGTGGATCAA
    ATGACAGCAA TTTGCAAGAA GAACGAGACG TCTGTAATCG ACCACATAGG AGACGAGTTG
    TTGTCTTGGT TCAGCAGTGG AGAGGAGGAG AAGATCAAGC AAGCAGTGGG CACTTTCTGC
    AGTAACCAGC CCTTCGCCCT GGAGCTCATC AAATCCCGAC AGAAGAAGGA CCAACGTTTC
    TCCTCCTTCA TGCAGGAGGC TGAAAGCAAT CGACTGTGTC GGCGTCTACA GCTAAAGGAC
    ATCATCCCTG CAGAGATGCA GAGGTTCACC AAATACCCAT TATTAATGGA CAACATTGCC
    AAATACACAG ATGAGTCAGA GGAAAAAGAC AAAGTGAGAC GAGCAGCAGA ATGCTGTCGA
    CAGATTCTCA ACCACGTCAA TCAGTCTGTG AAGGAGTCCG AGAATAAACA GAGACTGGAA
    GACTACCAGA GACGGCTGGA CCTCTCTTCC CTCAAGCAGA GCGAGAACCC AATGATCTCA
    GAGTTCAAGA ACCTGGATCT CACTAAACGA AAGATGGTCC ACGAGGGTCC TCTCTCCTGG
    AAGATAAATA AAGACAAAAC AATTGAGTTG TATACGTTAC TCCTGGAAGA CATACTGGTG
    CTGCTGCAGA AGCAGGACGA GCGTCTCATT CTCAGGTGTC ACAGTAAGAA CCTGGCAGGA
    ACAGCTGAAA CCAAACACAT CTTCAGCCCT ATCATCAAGC TCAGCACTGT GCTGGTGCGC
    TCTGTGGCCA CAGATAATAG ATCATTCTTT GTGATCTCCA TGTCCAAAAA CGGAGCTCAG
    ATTTATGAGC TGATGGCCCA GACGGTGTCT GAGCAGAGGA TGTGGCAACG GCAGATCACT
    CAGAGGGCAG AGGTTATGAA CACCAAACCT CACAGTGTCA TTCCTTTGCC TCAACACGAT
    GGCGACAGAG ACGGTGTGGA GTTAAGCAAG TTCAGCAAAG GCTCTGATCG GATCTCCACA
    GGAAGCATCC AGTCCGCAGA GAAAGACGGT GAATTTTCGC CCTCAGCAGC CCTGCGGGAC
    TGTGTGCCCG ATACCAACCC TGGGGAAGAG GACCAGGTCG CGAGAGCTGG CAGAGACGCT
    TCCTATCAGG ACTCTGAACT TTTGAACGGC AGGGAAACAG ACTCTCTATC TTGTTCATCT
    AAAGCTGACG AGGCCCTGAA AACATTGGCA GCGCTAAAGC AGGTGCTGCT GACTCAGCTG
    ATGGCTCAGG ATGAGGGTGA GCGATTGGGC CGATCGGCCG GCAGTCGTCT GCTCAGGACC
    ACCTCTTTAC GCACACCAGT GGAGCACAAC TCCAACCGCA CCCCTAGTCG AGCTCAGGAC
    TACACCTCTG GCGACACAGG CTTCTATGAG TGCCCGGAGG ATTACGCAGC GTACCTGGTT
    CTGGAGGGCT TCGGTGGCTC GGGCGAGAGC AGCACTGATG ATGATTTTCA GGTTGGGAGA
    AAGGAGCCTG GTTTGGCCCA CAGCTGCGCT GCTGACTCTG GGATCAGCCT GAAGTTCTCC
    TCGCCCTCCT CTGGCAGCAC GTCCCGTCTC AGCAGACAGG TGCTCTCACA CCTGCGCTGC
    CTGCAGGCCA ACCTCAACCA CCTCAAGGAG ATGGAAACTA AGTATTATAA TCTACTTCGC
    CAAAGGCCTG TCGGATCAGC AGCAGAAGCA GACGAGAGCA GAGAGAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43817
    Clone ID Related Accession (Same CDS sequence) XM_009291731.3 , XM_009291731.2
    Accession Version XM_009291731.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGACC TCCTGATGTG GGCACACCTA AACCGAGCAG AAGGTTTGAG
    GATCTGCAGA GTGAGGATGA TCAGGGGAGT GATGTTTACT CTGATGTAGA TCCTCCAAAC
    TGGCAGCAGC TGGTCGGGCG TGATGTTTTA GCAGGCCTGA CGCCCCACGA GATCAAGAGA
    CAAGAAGTCA TTAATGAGCT GTTCTACACG GAGCGAGCTC ACGTACGCAT GTTGAAGGTC
    CTAGACAAGG TCTTCTACCA GAAACTCAAC CGAGACAACA TACTGCCCCC TGCTGACATC
    AAGTACATAT TCACCAACCT GGAGGAAATC ATCCAACTCC ACGTGAGAAT AGTGGATCAA
    ATGACAGCAA TTTGCAAGAA GAACGAGACG TCTGTAATCG ACCACATAGG AGACGAGTTG
    TTGTCTTGGT TCAGCAGTGG AGAGGAGGAG AAGATCAAGC AAGCAGTGGG CACTTTCTGC
    AGTAACCAGC CCTTCGCCCT GGAGCTCATC AAATCCCGAC AGAAGAAGGA CCAACGTTTC
    TCCTCCTTCA TGCAGGAGGC TGAAAGCAAT CGACTGTGTC GGCGTCTACA GCTAAAGGAC
    ATCATCCCTG CAGAGATGCA GAGGTTCACC AAATACCCAT TATTAATGGA CAACATTGCC
    AAATACACAG ATGAGTCAGA GGAAAAAGAC AAAGTGAGAC GAGCAGCAGA ATGCTGTCGA
    CAGATTCTCA ACCACGTCAA TCAGTCTGTG AAGGAGTCCG AGAATAAACA GAGACTGGAA
    GACTACCAGA GACGGCTGGA CCTCTCTTCC CTCAAGCAGA GCGAGAACCC AATGATCTCA
    GAGTTCAAGA ACCTGGATCT CACTAAACGA AAGATGGTCC ACGAGGGTCC TCTCTCCTGG
    AAGATAAATA AAGACAAAAC AATTGAGTTG TATACGTTAC TCCTGGAAGA CATACTGGTG
    CTGCTGCAGA AGCAGGACGA GCGTCTCATT CTCAGGTGTC ACAGTAAGAA CCTGGCAGGA
    ACAGCTGAAA CCAAACACAT CTTCAGCCCT ATCATCAAGC TCAGCACTGT GCTGGTGCGC
    TCTGTGGCCA CAGATAATAG ATCATTCTTT GTGATCTCCA TGTCCAAAAA CGGAGCTCAG
    ATTTATGAGC TGATGGCCCA GACGGTGTCT GAGCAGAGGA TGTGGCAACG GCAGATCACT
    CAGAGGGCAG AGGTTATGAA CACCAAACCT CACAGTGTCA TTCCTTTGCC TCAACACGAT
    GGCGACAGAG ACGGTGTGGA GTTAAGCAAG TTCAGCAAAG GCTCTGATCG GATCTCCACA
    GGAAGCATCC AGTCCGCAGA GAAAGACGGT GAATTTTCGC CCTCAGCAGC CCTGCGGGAC
    TGTGTGCCCG ATACCAACCC TGGGGAAGAG GACCAGGTCG CGAGAGCTGG CAGAGACGCT
    TCCTATCAGG ACTCTGAACT TTTGAACGGC AGGGAAACAG ACTCTCTATC TTGTTCATCT
    AAAGCTGACG AGGCCCTGAA AACATTGGCA GCGCTAAAGC AGGTGCTGCT GACTCAGCTG
    ATGGCTCAGG ATGAGGGTGA GCGATTGGGC CGATCGGCCG GCAGTCGTCT GCTCAGGACC
    ACCTCTTTAC GCACACCAGT GGAGCACAAC TCCAACCGCA CCCCTAGTCG AGCTCAGGAC
    TACACCTCTG GCGACACAGG CTTCTATGAG TGCCCGGAGG ATTACGCAGC GTACCTGGTT
    CTGGAGGGCT TCGGTGGCTC GGGCGAGAGC AGCACTGATG ATGATTTTCA GGTTGGGAGA
    AAGGAGCCTG GTTTGGCCCA CAGCTGCGCT GCTGACTCTG GGATCAGCCT GAAGTTCTCC
    TCGCCCTCCT CTGGCAGCAC GTCCCGTCTC AGCAGACAGG TGCTCTCACA CCTGCGCTGC
    CTGCAGGCCA ACCTCAACCA CCTCAAGGAG ATGGAAACTA AGTATTATAA TCTACTTCGC
    CAAAGGCCTG TCGGATCAGC AGCAGAAGCA GACGAGAGCA GAGAGAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290006.1
    CDS908..4981
    Translation

    Target ORF information:

    RefSeq Version XM_009291731.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291731.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGACC TCCTGATGTG GGCACACCTA AACCGAGCAG AAGGTTTGAG
    GATCTGCAGA GTGAGGATGA TCAGGGGAGT GATGTTTACT CTGATGTAGA TCCTCCAAAC
    TGGCAGCAGC TGGTCGGGCG TGATGTTTTA GCAGGCCTGA CGCCCCACGA GATCAAGAGA
    CAAGAAGTCA TTAATGAGCT GTTCTACACG GAGCGAGCTC ACGTACGCAT GTTGAAGGTC
    CTAGACAAGG TCTTCTACCA GAAACTCAAC CGAGACAACA TACTGCCCCC TGCTGACATC
    AAGTACATAT TCACCAACCT GGAGGAAATC ATCCAACTCC ACGTGAGAAT AGTGGATCAA
    ATGACAGCAA TTTGCAAGAA GAACGAGACG TCTGTAATCG ACCACATAGG AGACGAGTTG
    TTGTCTTGGT TCAGCAGTGG AGAGGAGGAG AAGATCAAGC AAGCAGTGGG CACTTTCTGC
    AGTAACCAGC CCTTCGCCCT GGAGCTCATC AAATCCCGAC AGAAGAAGGA CCAACGTTTC
    TCCTCCTTCA TGCAGGAGGC TGAAAGCAAT CGACTGTGTC GGCGTCTACA GCTAAAGGAC
    ATCATCCCTG CAGAGATGCA GAGGTTCACC AAATACCCAT TATTAATGGA CAACATTGCC
    AAATACACAG ATGAGTCAGA GGAAAAAGAC AAAGTGAGAC GAGCAGCAGA ATGCTGTCGA
    CAGATTCTCA ACCACGTCAA TCAGTCTGTG AAGGAGTCCG AGAATAAACA GAGACTGGAA
    GACTACCAGA GACGGCTGGA CCTCTCTTCC CTCAAGCAGA GCGAGAACCC AATGATCTCA
    GAGTTCAAGA ACCTGGATCT CACTAAACGA AAGATGGTCC ACGAGGGTCC TCTCTCCTGG
    AAGATAAATA AAGACAAAAC AATTGAGTTG TATACGTTAC TCCTGGAAGA CATACTGGTG
    CTGCTGCAGA AGCAGGACGA GCGTCTCATT CTCAGGTGTC ACAGTAAGAA CCTGGCAGGA
    ACAGCTGAAA CCAAACACAT CTTCAGCCCT ATCATCAAGC TCAGCACTGT GCTGGTGCGC
    TCTGTGGCCA CAGATAATAG ATCATTCTTT GTGATCTCCA TGTCCAAAAA CGGAGCTCAG
    ATTTATGAGC TGATGGCCCA GACGGTGTCT GAGCAGAGGA TGTGGCAACG GCAGATCACT
    CAGAGGGCAG AGGTTATGAA CACCAAACCT CACAGTGTCA TTCCTTTGCC TCAACACGAT
    GGCGACAGAG ACGGTGTGGA GTTAAGCAAG TTCAGCAAAG GCTCTGATCG GATCTCCACA
    GGAAGCATCC AGTCCGCAGA GAAAGACGGT GAATTTTCGC CCTCAGCAGC CCTGCGGGAC
    TGTGTGCCCG ATACCAACCC TGGGGAAGAG GACCAGGTCG CGAGAGCTGG CAGAGACGCT
    TCCTATCAGG ACTCTGAACT TTTGAACGGC AGGGAAACAG ACTCTCTATC TTGTTCATCT
    AAAGCTGACG AGGCCCTGAA AACATTGGCA GCGCTAAAGC AGGTGCTGCT GACTCAGCTG
    ATGGCTCAGG ATGAGGGTGA GCGATTGGGC CGATCGGCCG GCAGTCGTCT GCTCAGGACC
    ACCTCTTTAC GCACACCAGT GGAGCACAAC TCCAACCGCA CCCCTAGTCG AGCTCAGGAC
    TACACCTCTG GCGACACAGG CTTCTATGAG TGCCCGGAGG ATTACGCAGC GTACCTGGTT
    CTGGAGGGCT TCGGTGGCTC GGGCGAGAGC AGCACTGATG ATGATTTTCA GGTTGGGAGA
    AAGGAGCCTG GTTTGGCCCA CAGCTGCGCT GCTGACTCTG GGATCAGCCT GAAGTTCTCC
    TCGCCCTCCT CTGGCAGCAC GTCCCGTCTC AGCAGACAGG TGCTCTCACA CCTGCGCTGC
    CTGCAGGCCA ACCTCAACCA CCTCAAGGAG ATGGAAACTA AGTATTATAA TCTACTTCGC
    CAAAGGCCTG TCGGATCAGC AGCAGAAGCA GACGAGAGCA GAGAGAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43818
    Clone ID Related Accession (Same CDS sequence) XM_009291732.3 , XM_009291732.2
    Accession Version XM_009291732.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGGTT TGAGGATCTG CAGAGTGAGG ATGATCAGGG GAGTGATGTT
    TACTCTGATG TAGATCCTCC AAACTGGCAG CAGCTGGTCG GGCGTGATGT TTTAGCAGGC
    CTGACGCCCC ACGAGATCAA GAGACAAGAA GTCATTAATG AGCTGTTCTA CACGGAGCGA
    GCTCACGTAC GCATGTTGAA GGTCCTAGAC AAGGTCTTCT ACCAGAAACT CAACCGAGAC
    AACATACTGC CCCCTGCTGA CATCAAGTAC ATATTCACCA ACCTGGAGGA AATCATCCAA
    CTCCACGTGA GAATAGTGGA TCAAATGACA GCAATTTGCA AGAAGAACGA GACGTCTGTA
    ATCGACCACA TAGGAGACGA GTTGTTGTCT TGGTTCAGCA GTGGAGAGGA GGAGAAGATC
    AAGCAAGCAG TGGGCACTTT CTGCAGTAAC CAGCCCTTCG CCCTGGAGCT CATCAAATCC
    CGACAGAAGA AGGACCAACG TTTCTCCTCC TTCATGCAGG AGGCTGAAAG CAATCGACTG
    TGTCGGCGTC TACAGCTAAA GGACATCATC CCTGCAGAGA TGCAGAGGTT CACCAAATAC
    CCATTATTAA TGGACAACAT TGCCAAATAC ACAGATGAGT CAGAGGAAAA AGACAAAGTG
    AGACGAGCAG CAGAATGCTG TCGACAGATT CTCAACCACG TCAATCAGTC TGTGAAGGAG
    TCCGAGAATA AACAGAGACT GGAAGACTAC CAGAGACGGC TGGACCTCTC TTCCCTCAAG
    CAGAGCGAGA ACCCAATGAT CTCAGAGTTC AAGAACCTGG ATCTCACTAA ACGAAAGATG
    GTCCACGAGG GTCCTCTCTC CTGGAAGATA AATAAAGACA AAACAATTGA GTTGTATACG
    TTACTCCTGG AAGACATACT GGTGCTGCTG CAGAAGCAGG ACGAGCGTCT CATTCTCAGG
    TGTCACAGTA AGAACCTGGC AGGAACAGCT GAAACCAAAC ACATCTTCAG CCCTATCATC
    AAGCTCAGCA CTGTGCTGGT GCGCTCTGTG GCCACAGATA ATAGATCATT CTTTGTGATC
    TCCATGTCCA AAAACGGAGC TCAGATTTAT GAGCTGATGG CCCAGACGGT GTCTGAGCAG
    AGGATGTGGC AACGGCAGAT CACTCAGAGG GCAGAGGTTA TGAACACCAA ACCTCACAGT
    GTCATTCCTT TGCCTCAACA CGATGGCGAC AGAGACGGTG TGGAGTTAAG CAAGTTCAGC
    AAAGGCTCTG ATCGGATCTC CACAGGAAGC ATCCAGTCCG CAGAGAAAGA CGGTGAATTT
    TCGCCCTCAG CAGCCCTGCG GGACTGTGTG CCCGATACCA ACCCTGGGGA AGAGGACCAG
    GTCGCGAGAG CTGGCAGAGA CGCTTCCTAT CAGGACTCTG AACTTTTGAA CGGCAGGGAA
    ACAGACTCTC TATCTTGTTC ATCTAAAGCT GACGAGGCCC TGAAAACATT GGCAGCGCTA
    AAGCAGGTGC TGCTGACTCA GCTGATGGCT CAGGATGAGG GTGAGCGATT GGGCCGATCG
    GCCGGCAGTC GTCTGCTCAG GACCACCTCT TTACGCACAC CAGTGGAGCA CAACTCCAAC
    CGCACCCCTA GTCGAGCTCA GGACTACACC TCTGGCGACA CAGGCTTCTA TGAGTGCCCG
    GAGGATTACG CAGCGTACCT GGTTCTGGAG GGCTTCGGTG GCTCGGGCGA GAGCAGCACT
    GATGATGATT TTCAGGTTGG GAGAAAGGAG CCTGGTTTGG CCCACAGCTG CGCTGCTGAC
    TCTGGGATCA GCCTGAAGTT CTCCTCGCCC TCCTCTGGCA GCACGTCCCG TCTCAGCAGA
    CAGGTGCTCT CACACCTGCG CTGCCTGCAG GCCAACCTCA ACCACCTCAA GGAGATGGAA
    ACTAAGTATT ATAATCTACT TCGCCAAAGG CCTGTCGGAT CAGCAGCAGA AGCAGACGAG
    AGCAGAGAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290007.1
    CDS903..4940
    Translation

    Target ORF information:

    RefSeq Version XM_009291732.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291732.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGGTT TGAGGATCTG CAGAGTGAGG ATGATCAGGG GAGTGATGTT
    TACTCTGATG TAGATCCTCC AAACTGGCAG CAGCTGGTCG GGCGTGATGT TTTAGCAGGC
    CTGACGCCCC ACGAGATCAA GAGACAAGAA GTCATTAATG AGCTGTTCTA CACGGAGCGA
    GCTCACGTAC GCATGTTGAA GGTCCTAGAC AAGGTCTTCT ACCAGAAACT CAACCGAGAC
    AACATACTGC CCCCTGCTGA CATCAAGTAC ATATTCACCA ACCTGGAGGA AATCATCCAA
    CTCCACGTGA GAATAGTGGA TCAAATGACA GCAATTTGCA AGAAGAACGA GACGTCTGTA
    ATCGACCACA TAGGAGACGA GTTGTTGTCT TGGTTCAGCA GTGGAGAGGA GGAGAAGATC
    AAGCAAGCAG TGGGCACTTT CTGCAGTAAC CAGCCCTTCG CCCTGGAGCT CATCAAATCC
    CGACAGAAGA AGGACCAACG TTTCTCCTCC TTCATGCAGG AGGCTGAAAG CAATCGACTG
    TGTCGGCGTC TACAGCTAAA GGACATCATC CCTGCAGAGA TGCAGAGGTT CACCAAATAC
    CCATTATTAA TGGACAACAT TGCCAAATAC ACAGATGAGT CAGAGGAAAA AGACAAAGTG
    AGACGAGCAG CAGAATGCTG TCGACAGATT CTCAACCACG TCAATCAGTC TGTGAAGGAG
    TCCGAGAATA AACAGAGACT GGAAGACTAC CAGAGACGGC TGGACCTCTC TTCCCTCAAG
    CAGAGCGAGA ACCCAATGAT CTCAGAGTTC AAGAACCTGG ATCTCACTAA ACGAAAGATG
    GTCCACGAGG GTCCTCTCTC CTGGAAGATA AATAAAGACA AAACAATTGA GTTGTATACG
    TTACTCCTGG AAGACATACT GGTGCTGCTG CAGAAGCAGG ACGAGCGTCT CATTCTCAGG
    TGTCACAGTA AGAACCTGGC AGGAACAGCT GAAACCAAAC ACATCTTCAG CCCTATCATC
    AAGCTCAGCA CTGTGCTGGT GCGCTCTGTG GCCACAGATA ATAGATCATT CTTTGTGATC
    TCCATGTCCA AAAACGGAGC TCAGATTTAT GAGCTGATGG CCCAGACGGT GTCTGAGCAG
    AGGATGTGGC AACGGCAGAT CACTCAGAGG GCAGAGGTTA TGAACACCAA ACCTCACAGT
    GTCATTCCTT TGCCTCAACA CGATGGCGAC AGAGACGGTG TGGAGTTAAG CAAGTTCAGC
    AAAGGCTCTG ATCGGATCTC CACAGGAAGC ATCCAGTCCG CAGAGAAAGA CGGTGAATTT
    TCGCCCTCAG CAGCCCTGCG GGACTGTGTG CCCGATACCA ACCCTGGGGA AGAGGACCAG
    GTCGCGAGAG CTGGCAGAGA CGCTTCCTAT CAGGACTCTG AACTTTTGAA CGGCAGGGAA
    ACAGACTCTC TATCTTGTTC ATCTAAAGCT GACGAGGCCC TGAAAACATT GGCAGCGCTA
    AAGCAGGTGC TGCTGACTCA GCTGATGGCT CAGGATGAGG GTGAGCGATT GGGCCGATCG
    GCCGGCAGTC GTCTGCTCAG GACCACCTCT TTACGCACAC CAGTGGAGCA CAACTCCAAC
    CGCACCCCTA GTCGAGCTCA GGACTACACC TCTGGCGACA CAGGCTTCTA TGAGTGCCCG
    GAGGATTACG CAGCGTACCT GGTTCTGGAG GGCTTCGGTG GCTCGGGCGA GAGCAGCACT
    GATGATGATT TTCAGGTTGG GAGAAAGGAG CCTGGTTTGG CCCACAGCTG CGCTGCTGAC
    TCTGGGATCA GCCTGAAGTT CTCCTCGCCC TCCTCTGGCA GCACGTCCCG TCTCAGCAGA
    CAGGTGCTCT CACACCTGCG CTGCCTGCAG GCCAACCTCA ACCACCTCAA GGAGATGGAA
    ACTAAGTATT ATAATCTACT TCGCCAAAGG CCTGTCGGAT CAGCAGCAGA AGCAGACGAG
    AGCAGAGAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43818
    Clone ID Related Accession (Same CDS sequence) XM_009291732.3 , XM_009291732.2
    Accession Version XM_009291732.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGGTT TGAGGATCTG CAGAGTGAGG ATGATCAGGG GAGTGATGTT
    TACTCTGATG TAGATCCTCC AAACTGGCAG CAGCTGGTCG GGCGTGATGT TTTAGCAGGC
    CTGACGCCCC ACGAGATCAA GAGACAAGAA GTCATTAATG AGCTGTTCTA CACGGAGCGA
    GCTCACGTAC GCATGTTGAA GGTCCTAGAC AAGGTCTTCT ACCAGAAACT CAACCGAGAC
    AACATACTGC CCCCTGCTGA CATCAAGTAC ATATTCACCA ACCTGGAGGA AATCATCCAA
    CTCCACGTGA GAATAGTGGA TCAAATGACA GCAATTTGCA AGAAGAACGA GACGTCTGTA
    ATCGACCACA TAGGAGACGA GTTGTTGTCT TGGTTCAGCA GTGGAGAGGA GGAGAAGATC
    AAGCAAGCAG TGGGCACTTT CTGCAGTAAC CAGCCCTTCG CCCTGGAGCT CATCAAATCC
    CGACAGAAGA AGGACCAACG TTTCTCCTCC TTCATGCAGG AGGCTGAAAG CAATCGACTG
    TGTCGGCGTC TACAGCTAAA GGACATCATC CCTGCAGAGA TGCAGAGGTT CACCAAATAC
    CCATTATTAA TGGACAACAT TGCCAAATAC ACAGATGAGT CAGAGGAAAA AGACAAAGTG
    AGACGAGCAG CAGAATGCTG TCGACAGATT CTCAACCACG TCAATCAGTC TGTGAAGGAG
    TCCGAGAATA AACAGAGACT GGAAGACTAC CAGAGACGGC TGGACCTCTC TTCCCTCAAG
    CAGAGCGAGA ACCCAATGAT CTCAGAGTTC AAGAACCTGG ATCTCACTAA ACGAAAGATG
    GTCCACGAGG GTCCTCTCTC CTGGAAGATA AATAAAGACA AAACAATTGA GTTGTATACG
    TTACTCCTGG AAGACATACT GGTGCTGCTG CAGAAGCAGG ACGAGCGTCT CATTCTCAGG
    TGTCACAGTA AGAACCTGGC AGGAACAGCT GAAACCAAAC ACATCTTCAG CCCTATCATC
    AAGCTCAGCA CTGTGCTGGT GCGCTCTGTG GCCACAGATA ATAGATCATT CTTTGTGATC
    TCCATGTCCA AAAACGGAGC TCAGATTTAT GAGCTGATGG CCCAGACGGT GTCTGAGCAG
    AGGATGTGGC AACGGCAGAT CACTCAGAGG GCAGAGGTTA TGAACACCAA ACCTCACAGT
    GTCATTCCTT TGCCTCAACA CGATGGCGAC AGAGACGGTG TGGAGTTAAG CAAGTTCAGC
    AAAGGCTCTG ATCGGATCTC CACAGGAAGC ATCCAGTCCG CAGAGAAAGA CGGTGAATTT
    TCGCCCTCAG CAGCCCTGCG GGACTGTGTG CCCGATACCA ACCCTGGGGA AGAGGACCAG
    GTCGCGAGAG CTGGCAGAGA CGCTTCCTAT CAGGACTCTG AACTTTTGAA CGGCAGGGAA
    ACAGACTCTC TATCTTGTTC ATCTAAAGCT GACGAGGCCC TGAAAACATT GGCAGCGCTA
    AAGCAGGTGC TGCTGACTCA GCTGATGGCT CAGGATGAGG GTGAGCGATT GGGCCGATCG
    GCCGGCAGTC GTCTGCTCAG GACCACCTCT TTACGCACAC CAGTGGAGCA CAACTCCAAC
    CGCACCCCTA GTCGAGCTCA GGACTACACC TCTGGCGACA CAGGCTTCTA TGAGTGCCCG
    GAGGATTACG CAGCGTACCT GGTTCTGGAG GGCTTCGGTG GCTCGGGCGA GAGCAGCACT
    GATGATGATT TTCAGGTTGG GAGAAAGGAG CCTGGTTTGG CCCACAGCTG CGCTGCTGAC
    TCTGGGATCA GCCTGAAGTT CTCCTCGCCC TCCTCTGGCA GCACGTCCCG TCTCAGCAGA
    CAGGTGCTCT CACACCTGCG CTGCCTGCAG GCCAACCTCA ACCACCTCAA GGAGATGGAA
    ACTAAGTATT ATAATCTACT TCGCCAAAGG CCTGTCGGAT CAGCAGCAGA AGCAGACGAG
    AGCAGAGAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290007.1
    CDS908..4945
    Translation

    Target ORF information:

    RefSeq Version XM_009291732.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGACG TGCTGGTGCA GAAGAGTGGA TGTGTAGTGG CTGACAGGAG ACCTGAGTCT
    TGTGGGCTAG TGCAGAGATG CGTGATCATT CAGAAAGATG AGAACGGCTT TGGGCTGACC
    GTCAGTGGGG ACAACCCTGT TTTCGTGCAG CTCGTCAAAC AGGATGGCGC TGCCATGCGA
    GCAGGTGTTC AGACGGGGGA CAGGATCATT AAGGTGAACG GGACCTTGGT AACGCAATCA
    AACCATGTTG AGGTGGTGAA GTTAATCAAA TCCGGCTCTT ATGTAGCTCT CACAGTATTA
    GGCCGACCGC CCGGGTTGCC CCAGATCCCC CTCCCTGAGG TGGACGTGGG TCTCGAGTCA
    CCGGATGTTT TCGGGGACCC TTTTATTACA TCTCCCAACT CTTACAATGC AGCAGAACGC
    ACCTCCTCTC CTCTTGTATC ATGGGAAGAG AACAGTGCTC TACACAACCA AAGAATGGAC
    AGCAGAAGGA CTGGTTTAAA GGATCAACAG GACACGCAGG AGAGTCCGTG TCTCAGGAAC
    AGTGAAGATG GCTCATGGGC GGAAGAGGAT CAATATAAGA GAGATTTCAG CCCAGAGGCT
    TTTTGGAGCG GAAATTCAGT GCCATCTGTC CACCGGCGCT TCTACCCAGC TTCTCCAGAG
    AGTCAGCGGG ACTCTAACTG TTCCAGTCCG AAAAGTACCC CCAGAGACAG CTTCAACTCC
    TGCCCTTCTC CTGAAGTTGA CTACGGCTCT GACCTGGATT CTCGATTCCC ATCCACCGTA
    GGGAGCCCAC GTTTGGTTTC TGAGATCATT GCTGCTGAAG ATGATGACTT TGACTCTGAG
    CAGGAGCAGG TTAATGGCCA GTGCAGCTGC TTCCAGAGCA TCCAGCTGCT CAAGACTCGA
    CCTGCTCATC TTGCTGCTTT TCTGCATCAT GTGATCTCTC AGTTTGACCC TGCACCTCTG
    CTCTGCTATC TGTATGCTGA CCTCTACAAA CAGACCAACT CCAAAGAAAC TCGGCGCATC
    TTCACGGACT TCTTGACGTT CTTCATAGAC CGGGGGGCAA ATTTGAAAGT CACTGTGCCT
    GAATCCATCT CGTCTGAACT CGAGCGGAGG AGACCAGAAT TGATTCCTGA GGAATTACAC
    AGGCAGTACA TACAGACAAT GCAGGAAACT CTCTTTCCTG ATGTACAGAG GAACCTGGAA
    GACTTCAGGC AGAAGCGTAG TATGGGCTTG ACCCTGGCGG ATACAGAGCT GTCTCGTCTG
    GACTCTGAAC AGCTGGCTGT AGACAGAGAG AGAGCCTGTG CTGAAAGCAT CCTCTCAAAG
    ATAGATGACG TTCTGTTGAC TTCACAACCC ACAGAAGAGG AGAAATGCTC CACTATACAG
    TATGTGATCT TCATGTACAT GAAGCATCTG GGAGTTCGGG CGAAAGAACA ACGAAACCTG
    GAATCCAAAC GAGTTCGAAT TAATTTCCTT CCTTCCAAAC TAAGGAAAAG CAAAACAGAA
    AAGGATGGGG AGGAGAAACT AAAAAAGTCT CGCTTCCCCA TCCGGGTGCC CCCGCGGTGG
    CCCGGTCGCC TCGATTCAGT ACCATCTGGT AGGGTCGGGG ACAGCATCAA GCAAAGACCT
    CAGAAGCAGA TGTCTCAGCC GGCACTGGGC GGCTCAGAGC TGATCGATGC CGGACGTCTG
    AGGGGCAGTC AGTCAAGTGA GGGCTCAGAC CTGCTCCACA GCCCAGGACT GATCACCTCT
    TCCCCCTTTA GTGCCCCCTA CAGCTCACCG TCAGACCCCT CTGGCAGGGA CTCTGAGTTC
    AATATGAGCC CGTCTGCGGT GGTTCCTCGG CTCAGTGATG GGCTGCAGTC TGGAGATCCA
    CTGGACAGCA TTTACCCACC CTGTGACTAC ATCCCATACA ACGTGGACCA GCTGGATGAA
    GACAGAGATG GAGACAGGTT TGAGGATCTG CAGAGTGAGG ATGATCAGGG GAGTGATGTT
    TACTCTGATG TAGATCCTCC AAACTGGCAG CAGCTGGTCG GGCGTGATGT TTTAGCAGGC
    CTGACGCCCC ACGAGATCAA GAGACAAGAA GTCATTAATG AGCTGTTCTA CACGGAGCGA
    GCTCACGTAC GCATGTTGAA GGTCCTAGAC AAGGTCTTCT ACCAGAAACT CAACCGAGAC
    AACATACTGC CCCCTGCTGA CATCAAGTAC ATATTCACCA ACCTGGAGGA AATCATCCAA
    CTCCACGTGA GAATAGTGGA TCAAATGACA GCAATTTGCA AGAAGAACGA GACGTCTGTA
    ATCGACCACA TAGGAGACGA GTTGTTGTCT TGGTTCAGCA GTGGAGAGGA GGAGAAGATC
    AAGCAAGCAG TGGGCACTTT CTGCAGTAAC CAGCCCTTCG CCCTGGAGCT CATCAAATCC
    CGACAGAAGA AGGACCAACG TTTCTCCTCC TTCATGCAGG AGGCTGAAAG CAATCGACTG
    TGTCGGCGTC TACAGCTAAA GGACATCATC CCTGCAGAGA TGCAGAGGTT CACCAAATAC
    CCATTATTAA TGGACAACAT TGCCAAATAC ACAGATGAGT CAGAGGAAAA AGACAAAGTG
    AGACGAGCAG CAGAATGCTG TCGACAGATT CTCAACCACG TCAATCAGTC TGTGAAGGAG
    TCCGAGAATA AACAGAGACT GGAAGACTAC CAGAGACGGC TGGACCTCTC TTCCCTCAAG
    CAGAGCGAGA ACCCAATGAT CTCAGAGTTC AAGAACCTGG ATCTCACTAA ACGAAAGATG
    GTCCACGAGG GTCCTCTCTC CTGGAAGATA AATAAAGACA AAACAATTGA GTTGTATACG
    TTACTCCTGG AAGACATACT GGTGCTGCTG CAGAAGCAGG ACGAGCGTCT CATTCTCAGG
    TGTCACAGTA AGAACCTGGC AGGAACAGCT GAAACCAAAC ACATCTTCAG CCCTATCATC
    AAGCTCAGCA CTGTGCTGGT GCGCTCTGTG GCCACAGATA ATAGATCATT CTTTGTGATC
    TCCATGTCCA AAAACGGAGC TCAGATTTAT GAGCTGATGG CCCAGACGGT GTCTGAGCAG
    AGGATGTGGC AACGGCAGAT CACTCAGAGG GCAGAGGTTA TGAACACCAA ACCTCACAGT
    GTCATTCCTT TGCCTCAACA CGATGGCGAC AGAGACGGTG TGGAGTTAAG CAAGTTCAGC
    AAAGGCTCTG ATCGGATCTC CACAGGAAGC ATCCAGTCCG CAGAGAAAGA CGGTGAATTT
    TCGCCCTCAG CAGCCCTGCG GGACTGTGTG CCCGATACCA ACCCTGGGGA AGAGGACCAG
    GTCGCGAGAG CTGGCAGAGA CGCTTCCTAT CAGGACTCTG AACTTTTGAA CGGCAGGGAA
    ACAGACTCTC TATCTTGTTC ATCTAAAGCT GACGAGGCCC TGAAAACATT GGCAGCGCTA
    AAGCAGGTGC TGCTGACTCA GCTGATGGCT CAGGATGAGG GTGAGCGATT GGGCCGATCG
    GCCGGCAGTC GTCTGCTCAG GACCACCTCT TTACGCACAC CAGTGGAGCA CAACTCCAAC
    CGCACCCCTA GTCGAGCTCA GGACTACACC TCTGGCGACA CAGGCTTCTA TGAGTGCCCG
    GAGGATTACG CAGCGTACCT GGTTCTGGAG GGCTTCGGTG GCTCGGGCGA GAGCAGCACT
    GATGATGATT TTCAGGTTGG GAGAAAGGAG CCTGGTTTGG CCCACAGCTG CGCTGCTGAC
    TCTGGGATCA GCCTGAAGTT CTCCTCGCCC TCCTCTGGCA GCACGTCCCG TCTCAGCAGA
    CAGGTGCTCT CACACCTGCG CTGCCTGCAG GCCAACCTCA ACCACCTCAA GGAGATGGAA
    ACTAAGTATT ATAATCTACT TCGCCAAAGG CCTGTCGGAT CAGCAGCAGA AGCAGACGAG
    AGCAGAGAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43819
    Clone ID Related Accession (Same CDS sequence) XM_009291733.3 , XM_009291733.2
    Accession Version XM_009291733.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGGGC TAGTGCAGAG ATGCGTGATC ATTCAGAAAG ATGAGAACGG CTTTGGGCTG
    ACCGTCAGTG GGGACAACCC TGTTTTCGTG CAGCTCGTCA AACAGGATGG CGCTGCCATG
    CGAGCAGGTG TTCAGACGGG GGACAGGATC ATTAAGGTGA ACGGGACCTT GGTAACGCAA
    TCAAACCATG TTGAGGTGGT GAAGTTAATC AAATCCGGCT CTTATGTAGC TCTCACAGTA
    TTAGGCCGAC CGCCCGGGTT GCCCCAGATC CCCCTCCCTG AGGTGGACGT GGGTCTCGAG
    TCACCGGATG TTTTCGGGGA CCCTTTTATT ACATCTCCCA ACTCTTACAA TGCAGCAGAA
    CGCACCTCCT CTCCTCTTGT ATCATGGGAA GAGAACAGTG CTCTACACAA CCAAAGAATG
    GACAGCAGAA GGACTGGTTT AAAGGATCAA CAGGACACGC AGGAGAGTCC GTGTCTCAGG
    AACAGTGAAG ATGGCTCATG GGCGGAAGAG GATCAATATA AGAGAGATTT CAGCCCAGAG
    GCTTTTTGGA GCGGAAATTC AGTGCCATCT GTCCACCGGC GCTTCTACCC AGCTTCTCCA
    GAGAGTCAGC GGGACTCTAA CTGTTCCAGT CCGAAAAGTA CCCCCAGAGA CAGCTTCAAC
    TCCTGCCCTT CTCCTGAAGT TGACTACGGC TCTGACCTGG ATTCTCGATT CCCATCCACC
    GTAGGGAGCC CACGTTTGGT TTCTGAGATC ATTGCTGCTG AAGATGATGA CTTTGACTCT
    GAGCAGGAGC AGGTTAATGG CCAGTGCAGC TGCTTCCAGA GCATCCAGCT GCTCAAGACT
    CGACCTGCTC ATCTTGCTGC TTTTCTGCAT CATGTGATCT CTCAGTTTGA CCCTGCACCT
    CTGCTCTGCT ATCTGTATGC TGACCTCTAC AAACAGACCA ACTCCAAAGA AACTCGGCGC
    ATCTTCACGG ACTTCTTGAC GTTCTTCATA GACCGGGGGG CAAATTTGAA AGTCACTGTG
    CCTGAATCCA TCTCGTCTGA ACTCGAGCGG AGGAGACCAG AATTGATTCC TGAGGAATTA
    CACAGGCAGT ACATACAGAC AATGCAGGAA ACTCTCTTTC CTGATGTACA GAGGAACCTG
    GAAGACTTCA GGCAGAAGCG TAGTATGGGC TTGACCCTGG CGGATACAGA GCTGTCTCGT
    CTGGACTCTG AACAGCTGGC TGTAGACAGA GAGAGAGCCT GTGCTGAAAG CATCCTCTCA
    AAGATAGATG ACGTTCTGTT GACTTCACAA CCCACAGAAG AGGAGAAATG CTCCACTATA
    CAGTATGTGA TCTTCATGTA CATGAAGCAT CTGGGAGTTC GGGCGAAAGA ACAACGAAAC
    CTGGAATCCA AACGAGTTCG AATTAATTTC CTTCCTTCCA AACTAAGGAA AAGCAAAACA
    GAAAAGGATG GGGAGGAGAA ACTAAAAAAG TCTCGCTTCC CCATCCGGGT GCCCCCGCGG
    TGGCCCGGTC GCCTCGATTC AGTACCATCT GGTAGGGTCG GGGACAGCAT CAAGCAAAGA
    CCTCAGAAGC AGATGTCTCA GCCGGCACTG GGCGGCTCAG AGCTGATCGA TGCCGGACGT
    CTGAGGGGCA GTCAGTCAAG TGAGGGCTCA GACCTGCTCC ACAGCCCAGG ACTGATCACC
    TCTTCCCCCT TTAGTGCCCC CTACAGCTCA CCGTCAGACC CCTCTGGCAG GGACTCTGAG
    TTCAATATGA GCCCGTCTGC GGTGGTTCCT CGGCTCAGTG ATGGGCTGCA GTCTGGAGAT
    CCACTGGACA GCATTTACCC ACCCTGTGAC TACATCCCAT ACAACGTGGA CCAGCTGGAT
    GAAGACAGAG ATGGAGACAG ACCTCCTGAT GTGGGCACAC CTAAACCGAG CAGAAGGTTT
    GAGGATCTGC AGAGTGAGGA TGATCAGGGG AGTGATGTTT ACTCTGATGT AGATCCTCCA
    AACTGGCAGC AGCTGGTCGG GCGTGATGTT TTAGCAGGCC TGACGCCCCA CGAGATCAAG
    AGACAAGAAG TCATTAATGA GCTGTTCTAC ACGGAGCGAG CTCACGTACG CATGTTGAAG
    GTCCTAGACA AGGTCTTCTA CCAGAAACTC AACCGAGACA ACATACTGCC CCCTGCTGAC
    ATCAAGTACA TATTCACCAA CCTGGAGGAA ATCATCCAAC TCCACGTGAG AATAGTGGAT
    CAAATGACAG CAATTTGCAA GAAGAACGAG ACGTCTGTAA TCGACCACAT AGGAGACGAG
    TTGTTGTCTT GGTTCAGCAG TGGAGAGGAG GAGAAGATCA AGCAAGCAGT GGGCACTTTC
    TGCAGTAACC AGCCCTTCGC CCTGGAGCTC ATCAAATCCC GACAGAAGAA GGACCAACGT
    TTCTCCTCCT TCATGCAGGA GGCTGAAAGC AATCGACTGT GTCGGCGTCT ACAGCTAAAG
    GACATCATCC CTGCAGAGAT GCAGAGGTTC ACCAAATACC CATTATTAAT GGACAACATT
    GCCAAATACA CAGATGAGTC AGAGGAAAAA GACAAAGTGA GACGAGCAGC AGAATGCTGT
    CGACAGATTC TCAACCACGT CAATCAGTCT GTGAAGGAGT CCGAGAATAA ACAGAGACTG
    GAAGACTACC AGAGACGGCT GGACCTCTCT TCCCTCAAGC AGAGCGAGAA CCCAATGATC
    TCAGAGTTCA AGAACCTGGA TCTCACTAAA CGAAAGATGG TCCACGAGGG TCCTCTCTCC
    TGGAAGATAA ATAAAGACAA AACAATTGAG TTGTATACGT TACTCCTGGA AGACATACTG
    GTGCTGCTGC AGAAGCAGGA CGAGCGTCTC ATTCTCAGGT GTCACAGTAA GAACCTGGCA
    GGAACAGCTG AAACCAAACA CATCTTCAGC CCTATCATCA AGCTCAGCAC TGTGCTGGTG
    CGCTCTGTGG CCACAGATAA TAGATCATTC TTTGTGATCT CCATGTCCAA AAACGGAGCT
    CAGATTTATG AGCTGATGGC CCAGACGGTG TCTGAGCAGA GGATGTGGCA ACGGCAGATC
    ACTCAGAGGG CAGAGGTTAT GAACACCAAA CCTCACAGTG TCATTCCTTT GCCTCAACAC
    GATGGCGACA GAGACGGTGT GGAGTTAAGC AAGTTCAGCA AAGGCTCTGA TCGGATCTCC
    ACAGGAAGCA TCCAGTCCGC AGAGAAAGAC GGTGAATTTT CGCCCTCAGC AGCCCTGCGG
    GACTGTGTGC CCGATACCAA CCCTGGGGAA GAGGACCAGG TCGCGAGAGC TGGCAGAGAC
    GCTTCCTATC AGGACTCTGA ACTTTTGAAC GGCAGGGAAA CAGACTCTCT ATCTTGTTCA
    TCTAAAGCTG ACGAGGCCCT GAAAACATTG GCAGCGCTAA AGCAGGTGCT GCTGACTCAG
    CTGATGGCTC AGGATGAGGG TGAGCGATTG GGCCGATCGG CCGGCAGTCG TCTGCTCAGG
    ACCACCTCTT TACGCACACC AGTGGAGCAC AACTCCAACC GCACCCCTAG TCGAGCTCAG
    GACTACACCT CTGGCGACAC AGGCTTCTAT GAGTGCCCGG AGGATTACGC AGCGTACCTG
    GTTCTGGAGG GCTTCGGTGG CTCGGGCGAG AGCAGCACTG ATGATGATTT TCAGGTTGGG
    AGAAAGGAGC CTGGTTTGGC CCACAGCTGC GCTGCTGACT CTGGGATCAG CCTGAAGTTC
    TCCTCGCCCT CCTCTGGCAG CACGTCCCGT CTCAGCAGAC AGGTGCTCTC ACACCTGCGC
    TGCCTGCAGG CCAACCTCAA CCACCTCAAG GAGATGGAAA CTAAGTATTA TAATCTACTT
    CGCCAAAGGC CTGTCGGATC AGCAGCAGAA GCAGACGAGA GCAGAGAGAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290008.1
    CDS903..4919
    Translation

    Target ORF information:

    RefSeq Version XM_009291733.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291733.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGGGC TAGTGCAGAG ATGCGTGATC ATTCAGAAAG ATGAGAACGG CTTTGGGCTG
    ACCGTCAGTG GGGACAACCC TGTTTTCGTG CAGCTCGTCA AACAGGATGG CGCTGCCATG
    CGAGCAGGTG TTCAGACGGG GGACAGGATC ATTAAGGTGA ACGGGACCTT GGTAACGCAA
    TCAAACCATG TTGAGGTGGT GAAGTTAATC AAATCCGGCT CTTATGTAGC TCTCACAGTA
    TTAGGCCGAC CGCCCGGGTT GCCCCAGATC CCCCTCCCTG AGGTGGACGT GGGTCTCGAG
    TCACCGGATG TTTTCGGGGA CCCTTTTATT ACATCTCCCA ACTCTTACAA TGCAGCAGAA
    CGCACCTCCT CTCCTCTTGT ATCATGGGAA GAGAACAGTG CTCTACACAA CCAAAGAATG
    GACAGCAGAA GGACTGGTTT AAAGGATCAA CAGGACACGC AGGAGAGTCC GTGTCTCAGG
    AACAGTGAAG ATGGCTCATG GGCGGAAGAG GATCAATATA AGAGAGATTT CAGCCCAGAG
    GCTTTTTGGA GCGGAAATTC AGTGCCATCT GTCCACCGGC GCTTCTACCC AGCTTCTCCA
    GAGAGTCAGC GGGACTCTAA CTGTTCCAGT CCGAAAAGTA CCCCCAGAGA CAGCTTCAAC
    TCCTGCCCTT CTCCTGAAGT TGACTACGGC TCTGACCTGG ATTCTCGATT CCCATCCACC
    GTAGGGAGCC CACGTTTGGT TTCTGAGATC ATTGCTGCTG AAGATGATGA CTTTGACTCT
    GAGCAGGAGC AGGTTAATGG CCAGTGCAGC TGCTTCCAGA GCATCCAGCT GCTCAAGACT
    CGACCTGCTC ATCTTGCTGC TTTTCTGCAT CATGTGATCT CTCAGTTTGA CCCTGCACCT
    CTGCTCTGCT ATCTGTATGC TGACCTCTAC AAACAGACCA ACTCCAAAGA AACTCGGCGC
    ATCTTCACGG ACTTCTTGAC GTTCTTCATA GACCGGGGGG CAAATTTGAA AGTCACTGTG
    CCTGAATCCA TCTCGTCTGA ACTCGAGCGG AGGAGACCAG AATTGATTCC TGAGGAATTA
    CACAGGCAGT ACATACAGAC AATGCAGGAA ACTCTCTTTC CTGATGTACA GAGGAACCTG
    GAAGACTTCA GGCAGAAGCG TAGTATGGGC TTGACCCTGG CGGATACAGA GCTGTCTCGT
    CTGGACTCTG AACAGCTGGC TGTAGACAGA GAGAGAGCCT GTGCTGAAAG CATCCTCTCA
    AAGATAGATG ACGTTCTGTT GACTTCACAA CCCACAGAAG AGGAGAAATG CTCCACTATA
    CAGTATGTGA TCTTCATGTA CATGAAGCAT CTGGGAGTTC GGGCGAAAGA ACAACGAAAC
    CTGGAATCCA AACGAGTTCG AATTAATTTC CTTCCTTCCA AACTAAGGAA AAGCAAAACA
    GAAAAGGATG GGGAGGAGAA ACTAAAAAAG TCTCGCTTCC CCATCCGGGT GCCCCCGCGG
    TGGCCCGGTC GCCTCGATTC AGTACCATCT GGTAGGGTCG GGGACAGCAT CAAGCAAAGA
    CCTCAGAAGC AGATGTCTCA GCCGGCACTG GGCGGCTCAG AGCTGATCGA TGCCGGACGT
    CTGAGGGGCA GTCAGTCAAG TGAGGGCTCA GACCTGCTCC ACAGCCCAGG ACTGATCACC
    TCTTCCCCCT TTAGTGCCCC CTACAGCTCA CCGTCAGACC CCTCTGGCAG GGACTCTGAG
    TTCAATATGA GCCCGTCTGC GGTGGTTCCT CGGCTCAGTG ATGGGCTGCA GTCTGGAGAT
    CCACTGGACA GCATTTACCC ACCCTGTGAC TACATCCCAT ACAACGTGGA CCAGCTGGAT
    GAAGACAGAG ATGGAGACAG ACCTCCTGAT GTGGGCACAC CTAAACCGAG CAGAAGGTTT
    GAGGATCTGC AGAGTGAGGA TGATCAGGGG AGTGATGTTT ACTCTGATGT AGATCCTCCA
    AACTGGCAGC AGCTGGTCGG GCGTGATGTT TTAGCAGGCC TGACGCCCCA CGAGATCAAG
    AGACAAGAAG TCATTAATGA GCTGTTCTAC ACGGAGCGAG CTCACGTACG CATGTTGAAG
    GTCCTAGACA AGGTCTTCTA CCAGAAACTC AACCGAGACA ACATACTGCC CCCTGCTGAC
    ATCAAGTACA TATTCACCAA CCTGGAGGAA ATCATCCAAC TCCACGTGAG AATAGTGGAT
    CAAATGACAG CAATTTGCAA GAAGAACGAG ACGTCTGTAA TCGACCACAT AGGAGACGAG
    TTGTTGTCTT GGTTCAGCAG TGGAGAGGAG GAGAAGATCA AGCAAGCAGT GGGCACTTTC
    TGCAGTAACC AGCCCTTCGC CCTGGAGCTC ATCAAATCCC GACAGAAGAA GGACCAACGT
    TTCTCCTCCT TCATGCAGGA GGCTGAAAGC AATCGACTGT GTCGGCGTCT ACAGCTAAAG
    GACATCATCC CTGCAGAGAT GCAGAGGTTC ACCAAATACC CATTATTAAT GGACAACATT
    GCCAAATACA CAGATGAGTC AGAGGAAAAA GACAAAGTGA GACGAGCAGC AGAATGCTGT
    CGACAGATTC TCAACCACGT CAATCAGTCT GTGAAGGAGT CCGAGAATAA ACAGAGACTG
    GAAGACTACC AGAGACGGCT GGACCTCTCT TCCCTCAAGC AGAGCGAGAA CCCAATGATC
    TCAGAGTTCA AGAACCTGGA TCTCACTAAA CGAAAGATGG TCCACGAGGG TCCTCTCTCC
    TGGAAGATAA ATAAAGACAA AACAATTGAG TTGTATACGT TACTCCTGGA AGACATACTG
    GTGCTGCTGC AGAAGCAGGA CGAGCGTCTC ATTCTCAGGT GTCACAGTAA GAACCTGGCA
    GGAACAGCTG AAACCAAACA CATCTTCAGC CCTATCATCA AGCTCAGCAC TGTGCTGGTG
    CGCTCTGTGG CCACAGATAA TAGATCATTC TTTGTGATCT CCATGTCCAA AAACGGAGCT
    CAGATTTATG AGCTGATGGC CCAGACGGTG TCTGAGCAGA GGATGTGGCA ACGGCAGATC
    ACTCAGAGGG CAGAGGTTAT GAACACCAAA CCTCACAGTG TCATTCCTTT GCCTCAACAC
    GATGGCGACA GAGACGGTGT GGAGTTAAGC AAGTTCAGCA AAGGCTCTGA TCGGATCTCC
    ACAGGAAGCA TCCAGTCCGC AGAGAAAGAC GGTGAATTTT CGCCCTCAGC AGCCCTGCGG
    GACTGTGTGC CCGATACCAA CCCTGGGGAA GAGGACCAGG TCGCGAGAGC TGGCAGAGAC
    GCTTCCTATC AGGACTCTGA ACTTTTGAAC GGCAGGGAAA CAGACTCTCT ATCTTGTTCA
    TCTAAAGCTG ACGAGGCCCT GAAAACATTG GCAGCGCTAA AGCAGGTGCT GCTGACTCAG
    CTGATGGCTC AGGATGAGGG TGAGCGATTG GGCCGATCGG CCGGCAGTCG TCTGCTCAGG
    ACCACCTCTT TACGCACACC AGTGGAGCAC AACTCCAACC GCACCCCTAG TCGAGCTCAG
    GACTACACCT CTGGCGACAC AGGCTTCTAT GAGTGCCCGG AGGATTACGC AGCGTACCTG
    GTTCTGGAGG GCTTCGGTGG CTCGGGCGAG AGCAGCACTG ATGATGATTT TCAGGTTGGG
    AGAAAGGAGC CTGGTTTGGC CCACAGCTGC GCTGCTGACT CTGGGATCAG CCTGAAGTTC
    TCCTCGCCCT CCTCTGGCAG CACGTCCCGT CTCAGCAGAC AGGTGCTCTC ACACCTGCGC
    TGCCTGCAGG CCAACCTCAA CCACCTCAAG GAGATGGAAA CTAAGTATTA TAATCTACTT
    CGCCAAAGGC CTGTCGGATC AGCAGCAGAA GCAGACGAGA GCAGAGAGAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43819
    Clone ID Related Accession (Same CDS sequence) XM_009291733.3 , XM_009291733.2
    Accession Version XM_009291733.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGGGC TAGTGCAGAG ATGCGTGATC ATTCAGAAAG ATGAGAACGG CTTTGGGCTG
    ACCGTCAGTG GGGACAACCC TGTTTTCGTG CAGCTCGTCA AACAGGATGG CGCTGCCATG
    CGAGCAGGTG TTCAGACGGG GGACAGGATC ATTAAGGTGA ACGGGACCTT GGTAACGCAA
    TCAAACCATG TTGAGGTGGT GAAGTTAATC AAATCCGGCT CTTATGTAGC TCTCACAGTA
    TTAGGCCGAC CGCCCGGGTT GCCCCAGATC CCCCTCCCTG AGGTGGACGT GGGTCTCGAG
    TCACCGGATG TTTTCGGGGA CCCTTTTATT ACATCTCCCA ACTCTTACAA TGCAGCAGAA
    CGCACCTCCT CTCCTCTTGT ATCATGGGAA GAGAACAGTG CTCTACACAA CCAAAGAATG
    GACAGCAGAA GGACTGGTTT AAAGGATCAA CAGGACACGC AGGAGAGTCC GTGTCTCAGG
    AACAGTGAAG ATGGCTCATG GGCGGAAGAG GATCAATATA AGAGAGATTT CAGCCCAGAG
    GCTTTTTGGA GCGGAAATTC AGTGCCATCT GTCCACCGGC GCTTCTACCC AGCTTCTCCA
    GAGAGTCAGC GGGACTCTAA CTGTTCCAGT CCGAAAAGTA CCCCCAGAGA CAGCTTCAAC
    TCCTGCCCTT CTCCTGAAGT TGACTACGGC TCTGACCTGG ATTCTCGATT CCCATCCACC
    GTAGGGAGCC CACGTTTGGT TTCTGAGATC ATTGCTGCTG AAGATGATGA CTTTGACTCT
    GAGCAGGAGC AGGTTAATGG CCAGTGCAGC TGCTTCCAGA GCATCCAGCT GCTCAAGACT
    CGACCTGCTC ATCTTGCTGC TTTTCTGCAT CATGTGATCT CTCAGTTTGA CCCTGCACCT
    CTGCTCTGCT ATCTGTATGC TGACCTCTAC AAACAGACCA ACTCCAAAGA AACTCGGCGC
    ATCTTCACGG ACTTCTTGAC GTTCTTCATA GACCGGGGGG CAAATTTGAA AGTCACTGTG
    CCTGAATCCA TCTCGTCTGA ACTCGAGCGG AGGAGACCAG AATTGATTCC TGAGGAATTA
    CACAGGCAGT ACATACAGAC AATGCAGGAA ACTCTCTTTC CTGATGTACA GAGGAACCTG
    GAAGACTTCA GGCAGAAGCG TAGTATGGGC TTGACCCTGG CGGATACAGA GCTGTCTCGT
    CTGGACTCTG AACAGCTGGC TGTAGACAGA GAGAGAGCCT GTGCTGAAAG CATCCTCTCA
    AAGATAGATG ACGTTCTGTT GACTTCACAA CCCACAGAAG AGGAGAAATG CTCCACTATA
    CAGTATGTGA TCTTCATGTA CATGAAGCAT CTGGGAGTTC GGGCGAAAGA ACAACGAAAC
    CTGGAATCCA AACGAGTTCG AATTAATTTC CTTCCTTCCA AACTAAGGAA AAGCAAAACA
    GAAAAGGATG GGGAGGAGAA ACTAAAAAAG TCTCGCTTCC CCATCCGGGT GCCCCCGCGG
    TGGCCCGGTC GCCTCGATTC AGTACCATCT GGTAGGGTCG GGGACAGCAT CAAGCAAAGA
    CCTCAGAAGC AGATGTCTCA GCCGGCACTG GGCGGCTCAG AGCTGATCGA TGCCGGACGT
    CTGAGGGGCA GTCAGTCAAG TGAGGGCTCA GACCTGCTCC ACAGCCCAGG ACTGATCACC
    TCTTCCCCCT TTAGTGCCCC CTACAGCTCA CCGTCAGACC CCTCTGGCAG GGACTCTGAG
    TTCAATATGA GCCCGTCTGC GGTGGTTCCT CGGCTCAGTG ATGGGCTGCA GTCTGGAGAT
    CCACTGGACA GCATTTACCC ACCCTGTGAC TACATCCCAT ACAACGTGGA CCAGCTGGAT
    GAAGACAGAG ATGGAGACAG ACCTCCTGAT GTGGGCACAC CTAAACCGAG CAGAAGGTTT
    GAGGATCTGC AGAGTGAGGA TGATCAGGGG AGTGATGTTT ACTCTGATGT AGATCCTCCA
    AACTGGCAGC AGCTGGTCGG GCGTGATGTT TTAGCAGGCC TGACGCCCCA CGAGATCAAG
    AGACAAGAAG TCATTAATGA GCTGTTCTAC ACGGAGCGAG CTCACGTACG CATGTTGAAG
    GTCCTAGACA AGGTCTTCTA CCAGAAACTC AACCGAGACA ACATACTGCC CCCTGCTGAC
    ATCAAGTACA TATTCACCAA CCTGGAGGAA ATCATCCAAC TCCACGTGAG AATAGTGGAT
    CAAATGACAG CAATTTGCAA GAAGAACGAG ACGTCTGTAA TCGACCACAT AGGAGACGAG
    TTGTTGTCTT GGTTCAGCAG TGGAGAGGAG GAGAAGATCA AGCAAGCAGT GGGCACTTTC
    TGCAGTAACC AGCCCTTCGC CCTGGAGCTC ATCAAATCCC GACAGAAGAA GGACCAACGT
    TTCTCCTCCT TCATGCAGGA GGCTGAAAGC AATCGACTGT GTCGGCGTCT ACAGCTAAAG
    GACATCATCC CTGCAGAGAT GCAGAGGTTC ACCAAATACC CATTATTAAT GGACAACATT
    GCCAAATACA CAGATGAGTC AGAGGAAAAA GACAAAGTGA GACGAGCAGC AGAATGCTGT
    CGACAGATTC TCAACCACGT CAATCAGTCT GTGAAGGAGT CCGAGAATAA ACAGAGACTG
    GAAGACTACC AGAGACGGCT GGACCTCTCT TCCCTCAAGC AGAGCGAGAA CCCAATGATC
    TCAGAGTTCA AGAACCTGGA TCTCACTAAA CGAAAGATGG TCCACGAGGG TCCTCTCTCC
    TGGAAGATAA ATAAAGACAA AACAATTGAG TTGTATACGT TACTCCTGGA AGACATACTG
    GTGCTGCTGC AGAAGCAGGA CGAGCGTCTC ATTCTCAGGT GTCACAGTAA GAACCTGGCA
    GGAACAGCTG AAACCAAACA CATCTTCAGC CCTATCATCA AGCTCAGCAC TGTGCTGGTG
    CGCTCTGTGG CCACAGATAA TAGATCATTC TTTGTGATCT CCATGTCCAA AAACGGAGCT
    CAGATTTATG AGCTGATGGC CCAGACGGTG TCTGAGCAGA GGATGTGGCA ACGGCAGATC
    ACTCAGAGGG CAGAGGTTAT GAACACCAAA CCTCACAGTG TCATTCCTTT GCCTCAACAC
    GATGGCGACA GAGACGGTGT GGAGTTAAGC AAGTTCAGCA AAGGCTCTGA TCGGATCTCC
    ACAGGAAGCA TCCAGTCCGC AGAGAAAGAC GGTGAATTTT CGCCCTCAGC AGCCCTGCGG
    GACTGTGTGC CCGATACCAA CCCTGGGGAA GAGGACCAGG TCGCGAGAGC TGGCAGAGAC
    GCTTCCTATC AGGACTCTGA ACTTTTGAAC GGCAGGGAAA CAGACTCTCT ATCTTGTTCA
    TCTAAAGCTG ACGAGGCCCT GAAAACATTG GCAGCGCTAA AGCAGGTGCT GCTGACTCAG
    CTGATGGCTC AGGATGAGGG TGAGCGATTG GGCCGATCGG CCGGCAGTCG TCTGCTCAGG
    ACCACCTCTT TACGCACACC AGTGGAGCAC AACTCCAACC GCACCCCTAG TCGAGCTCAG
    GACTACACCT CTGGCGACAC AGGCTTCTAT GAGTGCCCGG AGGATTACGC AGCGTACCTG
    GTTCTGGAGG GCTTCGGTGG CTCGGGCGAG AGCAGCACTG ATGATGATTT TCAGGTTGGG
    AGAAAGGAGC CTGGTTTGGC CCACAGCTGC GCTGCTGACT CTGGGATCAG CCTGAAGTTC
    TCCTCGCCCT CCTCTGGCAG CACGTCCCGT CTCAGCAGAC AGGTGCTCTC ACACCTGCGC
    TGCCTGCAGG CCAACCTCAA CCACCTCAAG GAGATGGAAA CTAAGTATTA TAATCTACTT
    CGCCAAAGGC CTGTCGGATC AGCAGCAGAA GCAGACGAGA GCAGAGAGAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290008.1
    CDS908..4924
    Translation

    Target ORF information:

    RefSeq Version XM_009291733.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12a (arhgef12a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGACA CACGGTCTAC AGTCAGTGAC AGAACCAGTA GCTTTCTCAG CAGAGACCAC 
    CCTCCCGACA AGAGGCCCAA AAGTGATAAG CTGCCCGGTA CCCCAACACA CGAGTTTGAC
    CCAACAGGGC TAGTGCAGAG ATGCGTGATC ATTCAGAAAG ATGAGAACGG CTTTGGGCTG
    ACCGTCAGTG GGGACAACCC TGTTTTCGTG CAGCTCGTCA AACAGGATGG CGCTGCCATG
    CGAGCAGGTG TTCAGACGGG GGACAGGATC ATTAAGGTGA ACGGGACCTT GGTAACGCAA
    TCAAACCATG TTGAGGTGGT GAAGTTAATC AAATCCGGCT CTTATGTAGC TCTCACAGTA
    TTAGGCCGAC CGCCCGGGTT GCCCCAGATC CCCCTCCCTG AGGTGGACGT GGGTCTCGAG
    TCACCGGATG TTTTCGGGGA CCCTTTTATT ACATCTCCCA ACTCTTACAA TGCAGCAGAA
    CGCACCTCCT CTCCTCTTGT ATCATGGGAA GAGAACAGTG CTCTACACAA CCAAAGAATG
    GACAGCAGAA GGACTGGTTT AAAGGATCAA CAGGACACGC AGGAGAGTCC GTGTCTCAGG
    AACAGTGAAG ATGGCTCATG GGCGGAAGAG GATCAATATA AGAGAGATTT CAGCCCAGAG
    GCTTTTTGGA GCGGAAATTC AGTGCCATCT GTCCACCGGC GCTTCTACCC AGCTTCTCCA
    GAGAGTCAGC GGGACTCTAA CTGTTCCAGT CCGAAAAGTA CCCCCAGAGA CAGCTTCAAC
    TCCTGCCCTT CTCCTGAAGT TGACTACGGC TCTGACCTGG ATTCTCGATT CCCATCCACC
    GTAGGGAGCC CACGTTTGGT TTCTGAGATC ATTGCTGCTG AAGATGATGA CTTTGACTCT
    GAGCAGGAGC AGGTTAATGG CCAGTGCAGC TGCTTCCAGA GCATCCAGCT GCTCAAGACT
    CGACCTGCTC ATCTTGCTGC TTTTCTGCAT CATGTGATCT CTCAGTTTGA CCCTGCACCT
    CTGCTCTGCT ATCTGTATGC TGACCTCTAC AAACAGACCA ACTCCAAAGA AACTCGGCGC
    ATCTTCACGG ACTTCTTGAC GTTCTTCATA GACCGGGGGG CAAATTTGAA AGTCACTGTG
    CCTGAATCCA TCTCGTCTGA ACTCGAGCGG AGGAGACCAG AATTGATTCC TGAGGAATTA
    CACAGGCAGT ACATACAGAC AATGCAGGAA ACTCTCTTTC CTGATGTACA GAGGAACCTG
    GAAGACTTCA GGCAGAAGCG TAGTATGGGC TTGACCCTGG CGGATACAGA GCTGTCTCGT
    CTGGACTCTG AACAGCTGGC TGTAGACAGA GAGAGAGCCT GTGCTGAAAG CATCCTCTCA
    AAGATAGATG ACGTTCTGTT GACTTCACAA CCCACAGAAG AGGAGAAATG CTCCACTATA
    CAGTATGTGA TCTTCATGTA CATGAAGCAT CTGGGAGTTC GGGCGAAAGA ACAACGAAAC
    CTGGAATCCA AACGAGTTCG AATTAATTTC CTTCCTTCCA AACTAAGGAA AAGCAAAACA
    GAAAAGGATG GGGAGGAGAA ACTAAAAAAG TCTCGCTTCC CCATCCGGGT GCCCCCGCGG
    TGGCCCGGTC GCCTCGATTC AGTACCATCT GGTAGGGTCG GGGACAGCAT CAAGCAAAGA
    CCTCAGAAGC AGATGTCTCA GCCGGCACTG GGCGGCTCAG AGCTGATCGA TGCCGGACGT
    CTGAGGGGCA GTCAGTCAAG TGAGGGCTCA GACCTGCTCC ACAGCCCAGG ACTGATCACC
    TCTTCCCCCT TTAGTGCCCC CTACAGCTCA CCGTCAGACC CCTCTGGCAG GGACTCTGAG
    TTCAATATGA GCCCGTCTGC GGTGGTTCCT CGGCTCAGTG ATGGGCTGCA GTCTGGAGAT
    CCACTGGACA GCATTTACCC ACCCTGTGAC TACATCCCAT ACAACGTGGA CCAGCTGGAT
    GAAGACAGAG ATGGAGACAG ACCTCCTGAT GTGGGCACAC CTAAACCGAG CAGAAGGTTT
    GAGGATCTGC AGAGTGAGGA TGATCAGGGG AGTGATGTTT ACTCTGATGT AGATCCTCCA
    AACTGGCAGC AGCTGGTCGG GCGTGATGTT TTAGCAGGCC TGACGCCCCA CGAGATCAAG
    AGACAAGAAG TCATTAATGA GCTGTTCTAC ACGGAGCGAG CTCACGTACG CATGTTGAAG
    GTCCTAGACA AGGTCTTCTA CCAGAAACTC AACCGAGACA ACATACTGCC CCCTGCTGAC
    ATCAAGTACA TATTCACCAA CCTGGAGGAA ATCATCCAAC TCCACGTGAG AATAGTGGAT
    CAAATGACAG CAATTTGCAA GAAGAACGAG ACGTCTGTAA TCGACCACAT AGGAGACGAG
    TTGTTGTCTT GGTTCAGCAG TGGAGAGGAG GAGAAGATCA AGCAAGCAGT GGGCACTTTC
    TGCAGTAACC AGCCCTTCGC CCTGGAGCTC ATCAAATCCC GACAGAAGAA GGACCAACGT
    TTCTCCTCCT TCATGCAGGA GGCTGAAAGC AATCGACTGT GTCGGCGTCT ACAGCTAAAG
    GACATCATCC CTGCAGAGAT GCAGAGGTTC ACCAAATACC CATTATTAAT GGACAACATT
    GCCAAATACA CAGATGAGTC AGAGGAAAAA GACAAAGTGA GACGAGCAGC AGAATGCTGT
    CGACAGATTC TCAACCACGT CAATCAGTCT GTGAAGGAGT CCGAGAATAA ACAGAGACTG
    GAAGACTACC AGAGACGGCT GGACCTCTCT TCCCTCAAGC AGAGCGAGAA CCCAATGATC
    TCAGAGTTCA AGAACCTGGA TCTCACTAAA CGAAAGATGG TCCACGAGGG TCCTCTCTCC
    TGGAAGATAA ATAAAGACAA AACAATTGAG TTGTATACGT TACTCCTGGA AGACATACTG
    GTGCTGCTGC AGAAGCAGGA CGAGCGTCTC ATTCTCAGGT GTCACAGTAA GAACCTGGCA
    GGAACAGCTG AAACCAAACA CATCTTCAGC CCTATCATCA AGCTCAGCAC TGTGCTGGTG
    CGCTCTGTGG CCACAGATAA TAGATCATTC TTTGTGATCT CCATGTCCAA AAACGGAGCT
    CAGATTTATG AGCTGATGGC CCAGACGGTG TCTGAGCAGA GGATGTGGCA ACGGCAGATC
    ACTCAGAGGG CAGAGGTTAT GAACACCAAA CCTCACAGTG TCATTCCTTT GCCTCAACAC
    GATGGCGACA GAGACGGTGT GGAGTTAAGC AAGTTCAGCA AAGGCTCTGA TCGGATCTCC
    ACAGGAAGCA TCCAGTCCGC AGAGAAAGAC GGTGAATTTT CGCCCTCAGC AGCCCTGCGG
    GACTGTGTGC CCGATACCAA CCCTGGGGAA GAGGACCAGG TCGCGAGAGC TGGCAGAGAC
    GCTTCCTATC AGGACTCTGA ACTTTTGAAC GGCAGGGAAA CAGACTCTCT ATCTTGTTCA
    TCTAAAGCTG ACGAGGCCCT GAAAACATTG GCAGCGCTAA AGCAGGTGCT GCTGACTCAG
    CTGATGGCTC AGGATGAGGG TGAGCGATTG GGCCGATCGG CCGGCAGTCG TCTGCTCAGG
    ACCACCTCTT TACGCACACC AGTGGAGCAC AACTCCAACC GCACCCCTAG TCGAGCTCAG
    GACTACACCT CTGGCGACAC AGGCTTCTAT GAGTGCCCGG AGGATTACGC AGCGTACCTG
    GTTCTGGAGG GCTTCGGTGG CTCGGGCGAG AGCAGCACTG ATGATGATTT TCAGGTTGGG
    AGAAAGGAGC CTGGTTTGGC CCACAGCTGC GCTGCTGACT CTGGGATCAG CCTGAAGTTC
    TCCTCGCCCT CCTCTGGCAG CACGTCCCGT CTCAGCAGAC AGGTGCTCTC ACACCTGCGC
    TGCCTGCAGG CCAACCTCAA CCACCTCAAG GAGATGGAAA CTAAGTATTA TAATCTACTT
    CGCCAAAGGC CTGTCGGATC AGCAGCAGAA GCAGACGAGA GCAGAGAGAA GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.