THBS1B cDNA ORF clone, Danio rerio(Zebrafish)

The following THBS1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa25039 XM_005160762.4
Latest version!
Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa25040 XM_685303.9
Latest version!
Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa25039 XM_005160762.3 Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa25040 XM_685303.8 Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa25039
    Clone ID Related Accession (Same CDS sequence) XM_005160762.4 , XM_005160762.3
    Accession Version XM_005160762.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product thrombospondin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160762.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT
    ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC
    AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT
    AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG
    ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC
    AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT
    TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT
    GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG
    GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT
    CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG
    ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC
    CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC
    CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG
    CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG
    CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC
    AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG
    ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG
    GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC
    ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC
    TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT
    TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT
    AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA
    AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA
    CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG
    AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA
    AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC
    GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC
    CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC
    AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG
    GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC
    GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA
    CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC
    CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT
    GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT
    AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA
    GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT
    CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG
    TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT
    CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT
    TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT
    GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG
    TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA
    GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC
    CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT
    GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA
    CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC
    GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT
    TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160819.1
    CDS187..3702
    Translation

    Target ORF information:

    RefSeq Version XM_005160762.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160762.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT
    ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC
    AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT
    AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG
    ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC
    AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT
    TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT
    GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG
    GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT
    CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG
    ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC
    CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC
    CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG
    CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG
    CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC
    AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG
    ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG
    GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC
    ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC
    TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT
    TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT
    AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA
    AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA
    CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG
    AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA
    AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC
    GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC
    CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC
    AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG
    GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC
    GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA
    CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC
    CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT
    GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT
    AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA
    GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT
    CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG
    TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT
    CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT
    TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT
    GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG
    TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA
    GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC
    CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT
    GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA
    CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC
    GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT
    TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25039
    Clone ID Related Accession (Same CDS sequence) XM_005160762.4 , XM_005160762.3
    Accession Version XM_005160762.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product thrombospondin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT
    ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC
    AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT
    AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG
    ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC
    AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT
    TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT
    GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG
    GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT
    CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG
    ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC
    CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC
    CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG
    CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG
    CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC
    AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG
    ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG
    GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC
    ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC
    TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT
    TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT
    AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA
    AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA
    CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG
    AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA
    AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC
    GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC
    CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC
    AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG
    GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC
    GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA
    CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC
    CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT
    GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT
    AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA
    GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT
    CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG
    TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT
    CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT
    TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT
    GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG
    TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA
    GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC
    CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT
    GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA
    CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC
    GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT
    TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160819.1
    CDS191..3706
    Translation

    Target ORF information:

    RefSeq Version XM_005160762.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160762.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT
    ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC
    AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT
    AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG
    ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC
    AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT
    TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT
    GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG
    GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT
    CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG
    ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC
    CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC
    CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG
    CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG
    CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC
    AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG
    ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG
    GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC
    ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC
    TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT
    TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT
    AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA
    AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA
    CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG
    AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA
    AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC
    GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC
    CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC
    AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG
    GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC
    GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA
    CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC
    CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT
    GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT
    AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA
    GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT
    CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG
    TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT
    CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT
    TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT
    GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG
    TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA
    GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC
    CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT
    GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA
    CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC
    GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT
    TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25040
    Clone ID Related Accession (Same CDS sequence) XM_685303.9 , XM_685303.8
    Accession Version XM_685303.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product thrombospondin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685303.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC
    CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA
    ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG
    GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG
    GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT
    GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC
    CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC
    ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT
    GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG
    CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC
    AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC
    TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT
    CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA
    ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC
    TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT
    AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT
    TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC
    CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT
    GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT
    GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC
    CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG
    AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC
    GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT
    GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT
    GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT
    CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT
    GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG
    AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC
    AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC
    ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC
    CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC
    AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG
    GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC
    AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT
    GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT
    TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG
    GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC
    CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT
    CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT
    GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC
    GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG
    AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG
    CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT
    TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG
    GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC
    ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC
    AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC
    TCAGACCTCA AATACGAATG CAGAGATGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690395.4
    CDS187..3699
    Translation

    Target ORF information:

    RefSeq Version XM_685303.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685303.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC
    CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA
    ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG
    GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG
    GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT
    GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC
    CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC
    ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT
    GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG
    CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC
    AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC
    TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT
    CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA
    ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC
    TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT
    AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT
    TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC
    CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT
    GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT
    GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC
    CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG
    AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC
    GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT
    GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT
    GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT
    CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT
    GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG
    AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC
    AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC
    ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC
    CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC
    AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG
    GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC
    AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT
    GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT
    TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG
    GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC
    CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT
    CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT
    GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC
    GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG
    AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG
    CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT
    TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG
    GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC
    ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC
    AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC
    TCAGACCTCA AATACGAATG CAGAGATGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25040
    Clone ID Related Accession (Same CDS sequence) XM_685303.9 , XM_685303.8
    Accession Version XM_685303.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product thrombospondin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685303.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC
    CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA
    ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG
    GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG
    GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT
    GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC
    CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC
    ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT
    GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG
    CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC
    AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC
    TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT
    CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA
    ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC
    TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT
    AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT
    TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC
    CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT
    GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT
    GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC
    CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG
    AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC
    GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT
    GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT
    GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT
    CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT
    GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG
    AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC
    AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC
    ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC
    CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC
    AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG
    GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC
    AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT
    GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT
    TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG
    GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC
    CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT
    CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT
    GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC
    GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG
    AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG
    CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT
    TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG
    GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC
    ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC
    AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC
    TCAGACCTCA AATACGAATG CAGAGATGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690395.4
    CDS191..3703
    Translation

    Target ORF information:

    RefSeq Version XM_685303.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685303.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
    GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG
    AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC
    CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC
    ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG
    GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC
    AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG
    TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA
    GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC
    TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA
    GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC
    ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC
    CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA
    ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG
    GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG
    GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT
    GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC
    CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC
    ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT
    GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG
    CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC
    AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC
    TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT
    CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA
    ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC
    TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT
    AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT
    TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC
    CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT
    GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT
    GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC
    CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG
    AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC
    GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT
    GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT
    GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT
    CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT
    GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG
    AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC
    AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC
    ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC
    CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC
    AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG
    GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC
    AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT
    GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT
    TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG
    GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC
    CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT
    CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT
    GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC
    GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG
    AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG
    CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT
    TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG
    GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC
    ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC
    AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC
    TCAGACCTCA AATACGAATG CAGAGATGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.