THBS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS1
Entrez Gene ID	281530
Full Name	thrombospondin 1
Synonyms	THBS
General protein information	Preferred Names thrombospondin 1 Names thrombospondin-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024997243.1	XP_024853011.1	thrombospondin-1 isoform X1
NM_174196.1	NP_776621.1	thrombospondin-1 precursor

Pathways from BioSystems

Homologies

	THBS1	XP_001093770.2
Mus musculus (house mouse)	Thbs1	NP_035710.2
Gallus gallus (chicken)	THBS1	NP_001186382.1
Pan troglodytes (chimpanzee)	THBS1	XP_510294.3
Canis lupus familiaris (dog)	THBS1	XP_544610.3
Bos taurus (cattle)	THBS1	NP_776621.1
Rattus norvegicus (Norway rat)	Thbs1	NP_001013080.1
Homo sapiens (human)	THBS1	NP_003237.2
Danio rerio (zebrafish)	thbs1	XP_005160819.1
Xenopus tropicalis (tropical clawed frog)	thbs1	XP_002937245.1

Gene Ontology
Bibliography

Related articles in PubMed

Fibroblast Growth Factor-2 and Transforming Growth Factor-beta1 Oppositely Regulate miR-221 that Targets Thrombospondin-1 in Bovine Luteal Endothelial Cells.
Farberov S, Meidan R
Biology of reproduction(2017 Dec)

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.
Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW
The Journal of reproduction and development62(5)501-510(2016 Oct)

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Farberov S, Meidan R
Biology of reproduction91(3)58(2014 Sep)

Thrombospondin-1-induced vascular smooth muscle cell migration is dependent on the hyaluronic acid receptor CD44.
Maier KG, Sadowitz B, Cullen S, Han X, Gahtan V
American journal of surgery198(5)664-9(2009 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

temporal expression and localization pattern of the thrombospondins and their specific receptors in the antral follicles and corpora lutea during the different physiological phases of the estrous cycle and induced luteolysis appear to be compatible with their inhibitory role in the control of ovarian angiogenesis
Title: Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.

Data suggest THBS1 expression predominates in luteal endothelial cells; THBS2 expression predominates in luteinized granulosa cells. Luteinization down-regulates expression of THBS1/THBS2, up-regulates expression of FGF2 (fibroblast growth factor 2).
Title: Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.

The novel finding that TSP-1-induced migration is dependent on the CD44 receptor links 2 pathways thought to be disparate (ie, TSP-1 and HyA).
Title: Thrombospondin-1-induced vascular smooth muscle cell migration is dependent on the hyaluronic acid receptor CD44.

The cell-specific regulation of TSP-1 suggests a potential mechanism for the aberrant angiogenesis in diabetics and TSP-1 involvement in development of various vascular diabetic complications
Title: Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose.

Preincubation of erythroid cells with thrombospondin 1 eliminated the inhibitory activity of insulin-like growth factor
Title: Thrombospondin 1, produced by endothelial cells under the action of erythropoietin, stimulates thymidine incorporation into erythroid cells and counteracts the inhibitory action of insulin-like growth factor binding protein 3.

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa45161	XM_024997243.1 Latest version!	Bos taurus thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa43367	NM_174196.1 Latest version!	Bos taurus thrombospondin 1 (THBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS1 ( XM_024997243.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853011.1 Bos taurus thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45161
Clone ID Related Accession (Same CDS sequence)	XM_024997243.1
Accession Version	XM_024997243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC 
TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG 
GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC 
AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC 
CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GACGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG CGGCTGGAGC 
CACTGGTCCC CATGGTCATC TTGCTCCGTA ACATGTGGAG ACGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACTCCTGC CCCATCAATG GAGGCTGGGG ACCTTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGTGGA GGAGGGGTAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CACCCCAGTT TGGAGGCAAG GACTGCATTG GTGATGTGAC AGAAAACCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAC GGATGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCCTGTCC CCCAGGCTAT 
AGTGGAGATG GAGTCGAGTG CAAAGACGTT GATGAGTGCA AAGAAGTCCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCGC GCTTCACTGG CTCGCAGCCC TTTGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAGCAGG TATGCAAGCC CCGAAACCCC TGCACAGACG GGACACACGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGCGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AATGAGGACC TGCTGTGCGT GGCCAACGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGCGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ACGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGTGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGATCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AATCTGGACA ACTGTCCCTA CGTGCCCAAC 
GCCAACCAGG CTGACCATGA CAAGGATGGC AAAGGCGATG CCTGTGACCA TGATGACGAC 
AATGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAACGCCGT GGACTTCAGT GGCACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAGTC CTACTGGGAC ACCAACCCCA CGAGGGCTCA GGGGTACTCT 
GGACTTTCCG TGAAGGTTGT AAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CTCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCATCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTAATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG GAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853011.1
CDS	195..3710
Translation	MGLAWGLGVLLLLHACGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVISNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFVTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPASDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDSCPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCIGDVTENQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVECKDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTSGQVRTLWHDPRHIGWKDFTAYRWHLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_024997243.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997243.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC 
TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG 
GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC 
AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC 
CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC 
GACGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC 
CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG 
ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG CGGCTGGAGC 
CACTGGTCCC CATGGTCATC TTGCTCCGTA ACATGTGGAG ACGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG 
GAGACCAAAG CCTGCCAGAA AGACTCCTGC CCCATCAATG GAGGCTGGGG ACCTTGGTCA 
CCATGGGACA TCTGTTCTGT CACCTGTGGA GGAGGGGTAC AGAAACGTAG CCGGCTCTGC 
AACAACCCCA CACCCCAGTT TGGAGGCAAG GACTGCATTG GTGATGTGAC AGAAAACCAG 
ATCTGCAACA AGCAGGACTG TCCCATTGAC GGATGCCTGT CCAATCCCTG CTTTGCTGGT 
GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCCTGTCC CCCAGGCTAT 
AGTGGAGATG GAGTCGAGTG CAAAGACGTT GATGAGTGCA AAGAAGTCCC TGATGCCTGC 
TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC 
TGCCCACCGC GCTTCACTGG CTCGCAGCCC TTTGGCCGGG GCGTGGAACA TGCCACCGCC 
AACAAGCAGG TATGCAAGCC CCGAAACCCC TGCACAGACG GGACACACGA CTGCAACAAG 
AACGCCAAGT GCAACTACCT GGGCCACTAC AGCGACCCCA TGTACCGCTG CGAGTGCAAG 
CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC 
AATGAGGACC TGCTGTGCGT GGCCAACGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC 
AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGCGAT 
GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ACGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGTGCAGCT 
GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG 
GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGATCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT 
CAGGATATTG ATGAAGACGG CCACCAGAAC AATCTGGACA ACTGTCCCTA CGTGCCCAAC 
GCCAACCAGG CTGACCATGA CAAGGATGGC AAAGGCGATG CCTGTGACCA TGATGACGAC 
AATGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG 
CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA 
CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGACGAAT TTAACGCCGT GGACTTCAGT GGCACCTTCT TCATCAACAC CGAGAGGGAT 
GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG 
TGGAAGCAAG TCACTCAGTC CTACTGGGAC ACCAACCCCA CGAGGGCTCA GGGGTACTCT 
GGACTTTCCG TGAAGGTTGT AAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC 
CTGTGGCACA CAGGAAACAC CTCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC 
ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCATCTGA GCCACAGGCC AAAGACAGGT 
TTCATCAGAG TGGTAATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT 
GACAAAACCT ATGCTGGTGG GAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC 
TTCTCCGACC TGAAATATGA ATGCAGAGAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( NM_174196.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776621.1 Bos taurus thrombospondin 1 (THBS1), mRNA.

Price & Availability↑

CloneID	OBa43367
Clone ID Related Accession (Same CDS sequence)	NM_174196.1
Accession Version	NM_174196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Bos taurus(cattle)
Product	thrombospondin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB005287.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB005287.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGCGGT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC 
TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG 
GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC 
AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC 
CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC 
TGGTCCCCAT GGTCATCTTG CTCCGTAACA TGTGGAGACG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CTCCTGCCCC ATCAATGGAG GCTGGGGACC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCAAAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTGACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC CATTGACGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CCTGTCCCCC AGGCTATAGT 
GGAGATGGAG TCGAGTGCAA AGACGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCGCGCT TCACTGGCTC GCAGCCCTTT GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAGCAGGTAT GCAAGCCCCG AAACCCCTGC ACAGACGGGA CACACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT 
GAGGACCTGC TGTGCGTGGC CAACGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTACG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG TGCAGCTGAC 
ATTGATGGGG ACAGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GATCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAT CTGGACAACT GTCCCTACGT GCCCAACGCC 
AACCAGGCTG ACCATGACAA GGATGGCAAA GGCGATGCCT GTGACCATGA TGACGACAAT 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ACGCCGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG GTACTCTGGA 
CTTTCCGTGA AGGTTGTAAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCTC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CATCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TAATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGGAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGACTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776621.1
CDS	148..3660
Translation	MGLAWGLGVLLLLHACGSNRIPESGGDNSVFDIFELTGAARKRSGRRLVKGPDPSSPAFRIEDANLIPPVPDKKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVISNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVFVTLDNNVVNGSSPAIRTDYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRTGDEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGKARETKACQKDSCPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPKPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVQCTSYPDGSWKCGACPPGYSGDGVECKDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGRGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLLCVANATYHCRKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDSILNERDNCQYVYNVDQKDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDQDKVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTSGQVRTLWHDPRHIGWKDFTAYRWHLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	NM_174196.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin 1 (THBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174196.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC 
CGCAAGCGGT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC 
TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG 
GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT 
GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC 
AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC 
ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC 
CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA 
GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC 
AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC 
TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT 
GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG 
CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG 
CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC 
TGGTCCCCAT GGTCATCTTG CTCCGTAACA TGTGGAGACG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG 
ACCAAAGCCT GCCAGAAAGA CTCCTGCCCC ATCAATGGAG GCTGGGGACC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGCCG GCTCTGCAAC 
AACCCCAAAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTGACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC CATTGACGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC 
CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CCTGTCCCCC AGGCTATAGT 
GGAGATGGAG TCGAGTGCAA AGACGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC 
CCACCGCGCT TCACTGGCTC GCAGCCCTTT GGCCGGGGCG TGGAACATGC CACCGCCAAC 
AAGCAGGTAT GCAAGCCCCG AAACCCCTGC ACAGACGGGA CACACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTACAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT 
GAGGACCTGC TGTGCGTGGC CAACGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT 
GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTACG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG TGCAGCTGAC 
ATTGATGGGG ACAGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC 
CAGAAAGACA CTGACATGGA CGGGGTTGGT GATCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG 
GATATTGATG AAGACGGCCA CCAGAACAAT CTGGACAACT GTCCCTACGT GCCCAACGCC 
AACCAGGCTG ACCATGACAA GGATGGCAAA GGCGATGCCT GTGACCATGA TGACGACAAT 
GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA 
GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GACGAATTTA ACGCCGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA GAGGGATGAC 
GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG GTACTCTGGA 
CTTTCCGTGA AGGTTGTAAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG 
TGGCACACAG GAAACACCTC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CATCTGAGCC ACAGGCCAAA GACAGGTTTC 
ATCAGAGTGG TAATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC 
AAAACCTATG CTGGTGGGAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCCGACCTGA AATATGAATG CAGAGACTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fibroblast Growth Factor-2 and Transforming Growth Factor-beta1 Oppositely Regulate miR-221 that Targets Thrombospondin-1 in Bovine Luteal Endothelial Cells.
Biology of reproduction(2017 Dec)
Farberov S,Meidan R

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.
The Journal of reproduction and development62(5)501-510(2016 Oct)
Berisha B,Schams D,Rodler D,Sinowatz F,Pfaffl MW

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Biology of reproduction91(3)58(2014 Sep)
Farberov S,Meidan R

Thrombospondin-1-induced vascular smooth muscle cell migration is dependent on the hyaluronic acid receptor CD44.
American journal of surgery198(5)664-9(2009 Nov)
Maier KG,Sadowitz B,Cullen S,Han X,Gahtan V

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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THBS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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