DNA2 cDNA ORF clone, Danio rerio(Zebrafish)

The following DNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145568 XM_682882.8
Latest version!
Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa18850 XM_021480834.1
Latest version!
Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa18850 XM_021474283.1
Latest version!
Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa18850 XM_682882.7
Latest version!
Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145568
    Clone ID Related Accession (Same CDS sequence) XM_682882.8
    Accession Version XM_682882.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682882.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGAAGT GCAAATCCAG CCGATCATCT ATGGTCAGTG GAGTTCAATC AAACATTTCT 
    TCCTTTTTCC TCTCAAAGGT AGCGGTAAAG TCACCTAAAA AATGTGAAGT AGGAATTACA
    AGATCCCCTG TTTCTTTAAA GACTGGGTTT GTGAAGGACG AAAATGTTCC CTGCTCCCCA
    AACCCATCTG CTGTCCCAGA GACGCCTGAC AGTCTGAGAA TCGAGAGGCC TTCAGACACT
    TCATTTGAGA AACGCTCACC TGTTTCACGT GGTCTACATA CTAAAGGAGT CAGGGGTAAA
    CCTAGCCTGA ATGCACGAAA GCTGTTTCAG CCACAGAAAA ACAGCAGTTT AGAGTCAGAG
    GACACTTTTG AGTTCAAGAA TGGCGTAAGT CACTCAGGCT CCGTAAAGAG GTTGTTGCAC
    AGCACTGAAA ACATACAGAG TGCCAAGAAG TCCAGGACTA CTCAAGATCA GAAAATAACT
    GATTGTGGCC CAAGCTCAGC AACGGCCAGT CAAAATAAAT TTTCTGAAAC ATTGCAAAAA
    GCTTTCTCTA AGAGTCCTGA GAAAAAAATC AAAACTGAGT CTGTGAGTTT GGAGGGTAGC
    CGACTGAAGG CTGGACCTTG TTTGAAAATG AATGAAAAAT TAAATGGTTT CTCAAAAGAC
    TCAGGAAGAT CCTCTCATCA TCTGAGCTTT AGTACTAGTG AGAAGGAGAA TTCACCTACT
    TGTAATGTAA AGTCAGAGGC AGACATGGGT GCCGTTTCTG GAACAAATGA CTTTGATCTT
    GTGATGTCGT ATAGTTCTTG GATACAAAAT GGATCTACTG AAGAAAAAGG TACTTTTCTT
    AACTGTCCAA TTGCTAAGAA GAGGCCCTTA AATACATCTG AGCTGCAAGT GAAAGAACAA
    ATTACTGATG GAAAGACTTT GCCTGTACCA TTTGAGGACG ATTTTGCCGA TTTGATGGAC
    GATTGGTTTG ATGAAGGGAT GGAGCAAAAA TCTGAGGCAC CAAAACCCAA GAAGTTCAGT
    CATGAAAAAA ACAAAGGCAT ACCTGAGCAT GTTGTGCTGG CCTCTGGTGT TCATAACCGA
    TATTGGGTGC TGGATGTGCA GGACGTTACC TCGTCAAGAA GCTGCTCAGA GAAGCACTTG
    ACCATCACTT GCTCAAAGAC GAGTCAGGCC ACAGAGACCT GCATTCTCAA AGATGGCTGG
    GAAAGTACTC TGGTAACTGT AGGGGACATT ATTCACTTGG AAGGGAAGTG TGTTTCCGGC
    TTGTGGACAA TTGATAGAGA CTCTGCTTTT CTGATTCTTC TGCCTGACCT GCTGATATCT
    GGGACCAGCA TTGCCAGCAG CATTCGCTGT ATGAGGCGAG GGGTGCTGGG AGAGATGTTC
    AAGGCATTTG ATGGTGGATC CAAGCAGATG TTAAATGGTA CAATAGTTCA TGATATCTTT
    CAAAAAGCAG CCATGTCCTC AGATTTCTCC TCAGAGAAGA TACAGACATT TGCCACGGAG
    GCCTTAAGAA GCCCCAACTA CCTCGGACAA ATGTACAGTC TAAAGCTGAC TCAGGCTGAC
    ATGAGACAGG AAGTTGAAGA ATATCTTCCC TCTATATCTG AATGGGCAAA ACAATACCTC
    CACACTTCCC CACAGGCAGG ACAAAAACAG CTGACTCTAA AACTCCCTAG TGATGGGGCT
    CTGAGCAAAC AAGATGCTTC CAGTAGCGTG ATGGTTACTG ATTTTGTGGA CATTGAGGAG
    AACATTTGGT GTCCACGTTT TGGTCTGAAG GGAAAGATTG ATGTCACAGC TGGAGTTAAG
    ATTCACCGGA GAGGCAGGAA GCCCATGGAA AGAATTGTGC CACTGGAGCT GAAAACTGGC
    AAAGAGTCTA ACTCCATTGA GCATCGCAGT CAGGTTATTC TGTACACACT GATGAGCTCA
    GCTAGACGTT GTGATCCAGA GGCAGGTTTC CTTGTCTACT TGAAGACTGG CAGTCTACAT
    CCTGTTGTGG GCAATCACAT GGACAGGAGA GAGCTGATTA AAATAAGGAA CTCATTGGCC
    CATCACATTG GCAACAACCT GATCAAGGTG GATGGGAAAA GTCAAATGGC CCAATTGCCT
    GCCATTGTAT CTGACCAGCA AACTTGCAAA TATTGTCCTC AGAAAAGGAA CTGTGCAATT
    TACAACAGAG CTGTGGAGAG AGATCCTGTG GAGAAGTGCA GTGAAAATCC ACAGGCCTTT
    GTGCAGTCAG AGAGTGAACA CCTCAGTCAG GTGCACCTCC AGTACTTTAG CCACTGGCTG
    CTGCTCTGCT CACTGGAGGC TCTCACCATG GAGAAAAAAG GAGGTCGGCG TAATATCTGG
    CTTCAGACTG CTCAACAGAG GGAGAAAATT GGTGGCTGTG CGGGTAACAT GCGGTTAGCT
    GGAGGCGTTA GCATTCTGTC TGATGGTGTT TACAAGCACA GCTTTGAGAG GAAGGAGGGC
    GAGCAGACTC TTACTGGCCT GATAGTGGGT GATAGGATTG TTGTCAGTGA TCAGGACTCT
    GTGCTCATTG GCCTGTCCAC CGGATACGTC ACAGAAATTG CAGTTAACCG AGTTACTTGC
    TCGCTGGACA AAAACCTTTC CAAGTATTCC TCAAACACTG TTTTCCGACT GGACCAGGAT
    GAAGGTGCAG GTGGTCTTAG CACTCATCTC GGAAACCTTT CTATGTTAAT GGAAAACACT
    CCAACAAGTG AAAGACTGAG AGAGTTGATT GTGGAATTCA GGCCTCCTCA GTTCATTGAC
    AGCCTCAGCA GTGTTTTGCC ACGAGATGCC AAGGACATTG TGGCCAACAT TCTGAAAGGA
    CTCAACAAAC CCCAGAAACA AGCAATGAAA AAAGTGCTTC TGTCTAAAGA TTACACGCTT
    ATTGTGGGAA TGCCGGGTAC TGGGAAAACT ACCACCATCT GTACACTGGT GCGAATTCTC
    CATGCTTGTG GCTTCAGTGT TCTTCTGACT AGCTACACCC ACTCTGCTGT GGACAACATC
    CTGCTGAAAC TGAAGAGGTT TAAGATCGGC TTTCTGAGAC TGGGTCGACC TCAAAAAGTC
    CATCATGAGA TCCTGCCTTT CACAGAGGAG GCATGCCGTG CCAAAGGCAT CCAAACTCTA
    GAGGAGCTGG AACAACTGTA CAGCAGAGAG CTGATTGTGG CCACGACTTG TATGGGTGTT
    AAACACCCAA TTTTCAGCCG CAGGCGCTTT GACTTCTGCA TCGTTGATGA GGCGTCTCAG
    ATCAGTCAGC CTGTGTGTAT AGGTCCACTG TTCTACGCCC AGCGCTTTGT CTTGGTGGGA
    GACCATCAAC AGCTTCCTCC TATTGTGCAG AATGCTGAGG CCAGGTCCTT GGGCATGGAT
    GAGAGTTTGT TCAAACGTCT GGAACACCAC AGGGATGCTG TTGTTCAGCT GAATGTGCAG
    TACAGGATGA ACAGTGCGAT CATGTCTCTC AGCAATGCTC TGATGTATGA GGGCCGGCTG
    GAGTGTGGCT CTGAGAAGAC GGCAAACGCA GTGCTTCAGC TGCCCAGTAG AGCTCAGGCA
    GAGGAGGAGC TGGAGCTGTA CGTGTGTCAG CCGCAGTACA GTGCTTGGGT CCAGGCCGCA
    CTGGAGCCCA ACAGTCCTGT CTGTTTCCTG GACACCTCTG AGGTTGCAGC CCCTGAAACT
    GTGGAGAAAA GTGGGATCAG TAATCACACT GAAGCTGTTT TAGTACAAGG CCTTGTTACT
    CTGCTGCTTA AGGCTGGATG CAGAGCATGT GATATTGGGG TCATCGCTCC GTATCGACAA
    CAGCTGAAAG TTATCTCCAG TCTGCTTCAG GGAGACGCCT TTAAAGCACT TGAAGTCAAC
    ACTGTGGACA AATACCAAGG ACGTGACAAG AGCGTCATCA TCGTGTCTTT TGTTAGAAGT
    AATGCTGAAG GCAACCTGGG AGAGTTGCTC CAGGACTGGC GTAGGCTGAA TGTGGCCATC
    ACTAGAGCCA AACACAAGCT CCTCATGCTG GGCTCTGCCC CCACTCTTCG CCGGTACGCA
    CCGCTGGAGA AACTTCTCTG CCACCTTCAG CAGGAAGATA TGATCTTCCA GCTACCTCCT
    GCTGCACACG AGGGACTACC TGGAGTCATC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687974.6
    CDS299..4414
    Translation

    Target ORF information:

    RefSeq Version XM_682882.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_682882.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGATGAAGT GCAAATCCAG CCGATCATCT ATGGTCAGTG GAGTTCAATC AAACATTTCT 
    TCCTTTTTCC TCTCAAAGGT AGCGGTAAAG TCACCTAAAA AATGTGAAGT AGGAATTACA
    AGATCCCCTG TTTCTTTAAA GACTGGGTTT GTGAAGGACG AAAATGTTCC CTGCTCCCCA
    AACCCATCTG CTGTCCCAGA GACGCCTGAC AGTCTGAGAA TCGAGAGGCC TTCAGACACT
    TCATTTGAGA AACGCTCACC TGTTTCACGT GGTCTACATA CTAAAGGAGT CAGGGGTAAA
    CCTAGCCTGA ATGCACGAAA GCTGTTTCAG CCACAGAAAA ACAGCAGTTT AGAGTCAGAG
    GACACTTTTG AGTTCAAGAA TGGCGTAAGT CACTCAGGCT CCGTAAAGAG GTTGTTGCAC
    AGCACTGAAA ACATACAGAG TGCCAAGAAG TCCAGGACTA CTCAAGATCA GAAAATAACT
    GATTGTGGCC CAAGCTCAGC AACGGCCAGT CAAAATAAAT TTTCTGAAAC ATTGCAAAAA
    GCTTTCTCTA AGAGTCCTGA GAAAAAAATC AAAACTGAGT CTGTGAGTTT GGAGGGTAGC
    CGACTGAAGG CTGGACCTTG TTTGAAAATG AATGAAAAAT TAAATGGTTT CTCAAAAGAC
    TCAGGAAGAT CCTCTCATCA TCTGAGCTTT AGTACTAGTG AGAAGGAGAA TTCACCTACT
    TGTAATGTAA AGTCAGAGGC AGACATGGGT GCCGTTTCTG GAACAAATGA CTTTGATCTT
    GTGATGTCGT ATAGTTCTTG GATACAAAAT GGATCTACTG AAGAAAAAGG TACTTTTCTT
    AACTGTCCAA TTGCTAAGAA GAGGCCCTTA AATACATCTG AGCTGCAAGT GAAAGAACAA
    ATTACTGATG GAAAGACTTT GCCTGTACCA TTTGAGGACG ATTTTGCCGA TTTGATGGAC
    GATTGGTTTG ATGAAGGGAT GGAGCAAAAA TCTGAGGCAC CAAAACCCAA GAAGTTCAGT
    CATGAAAAAA ACAAAGGCAT ACCTGAGCAT GTTGTGCTGG CCTCTGGTGT TCATAACCGA
    TATTGGGTGC TGGATGTGCA GGACGTTACC TCGTCAAGAA GCTGCTCAGA GAAGCACTTG
    ACCATCACTT GCTCAAAGAC GAGTCAGGCC ACAGAGACCT GCATTCTCAA AGATGGCTGG
    GAAAGTACTC TGGTAACTGT AGGGGACATT ATTCACTTGG AAGGGAAGTG TGTTTCCGGC
    TTGTGGACAA TTGATAGAGA CTCTGCTTTT CTGATTCTTC TGCCTGACCT GCTGATATCT
    GGGACCAGCA TTGCCAGCAG CATTCGCTGT ATGAGGCGAG GGGTGCTGGG AGAGATGTTC
    AAGGCATTTG ATGGTGGATC CAAGCAGATG TTAAATGGTA CAATAGTTCA TGATATCTTT
    CAAAAAGCAG CCATGTCCTC AGATTTCTCC TCAGAGAAGA TACAGACATT TGCCACGGAG
    GCCTTAAGAA GCCCCAACTA CCTCGGACAA ATGTACAGTC TAAAGCTGAC TCAGGCTGAC
    ATGAGACAGG AAGTTGAAGA ATATCTTCCC TCTATATCTG AATGGGCAAA ACAATACCTC
    CACACTTCCC CACAGGCAGG ACAAAAACAG CTGACTCTAA AACTCCCTAG TGATGGGGCT
    CTGAGCAAAC AAGATGCTTC CAGTAGCGTG ATGGTTACTG ATTTTGTGGA CATTGAGGAG
    AACATTTGGT GTCCACGTTT TGGTCTGAAG GGAAAGATTG ATGTCACAGC TGGAGTTAAG
    ATTCACCGGA GAGGCAGGAA GCCCATGGAA AGAATTGTGC CACTGGAGCT GAAAACTGGC
    AAAGAGTCTA ACTCCATTGA GCATCGCAGT CAGGTTATTC TGTACACACT GATGAGCTCA
    GCTAGACGTT GTGATCCAGA GGCAGGTTTC CTTGTCTACT TGAAGACTGG CAGTCTACAT
    CCTGTTGTGG GCAATCACAT GGACAGGAGA GAGCTGATTA AAATAAGGAA CTCATTGGCC
    CATCACATTG GCAACAACCT GATCAAGGTG GATGGGAAAA GTCAAATGGC CCAATTGCCT
    GCCATTGTAT CTGACCAGCA AACTTGCAAA TATTGTCCTC AGAAAAGGAA CTGTGCAATT
    TACAACAGAG CTGTGGAGAG AGATCCTGTG GAGAAGTGCA GTGAAAATCC ACAGGCCTTT
    GTGCAGTCAG AGAGTGAACA CCTCAGTCAG GTGCACCTCC AGTACTTTAG CCACTGGCTG
    CTGCTCTGCT CACTGGAGGC TCTCACCATG GAGAAAAAAG GAGGTCGGCG TAATATCTGG
    CTTCAGACTG CTCAACAGAG GGAGAAAATT GGTGGCTGTG CGGGTAACAT GCGGTTAGCT
    GGAGGCGTTA GCATTCTGTC TGATGGTGTT TACAAGCACA GCTTTGAGAG GAAGGAGGGC
    GAGCAGACTC TTACTGGCCT GATAGTGGGT GATAGGATTG TTGTCAGTGA TCAGGACTCT
    GTGCTCATTG GCCTGTCCAC CGGATACGTC ACAGAAATTG CAGTTAACCG AGTTACTTGC
    TCGCTGGACA AAAACCTTTC CAAGTATTCC TCAAACACTG TTTTCCGACT GGACCAGGAT
    GAAGGTGCAG GTGGTCTTAG CACTCATCTC GGAAACCTTT CTATGTTAAT GGAAAACACT
    CCAACAAGTG AAAGACTGAG AGAGTTGATT GTGGAATTCA GGCCTCCTCA GTTCATTGAC
    AGCCTCAGCA GTGTTTTGCC ACGAGATGCC AAGGACATTG TGGCCAACAT TCTGAAAGGA
    CTCAACAAAC CCCAGAAACA AGCAATGAAA AAAGTGCTTC TGTCTAAAGA TTACACGCTT
    ATTGTGGGAA TGCCGGGTAC TGGGAAAACT ACCACCATCT GTACACTGGT GCGAATTCTC
    CATGCTTGTG GCTTCAGTGT TCTTCTGACT AGCTACACCC ACTCTGCTGT GGACAACATC
    CTGCTGAAAC TGAAGAGGTT TAAGATCGGC TTTCTGAGAC TGGGTCGACC TCAAAAAGTC
    CATCATGAGA TCCTGCCTTT CACAGAGGAG GCATGCCGTG CCAAAGGCAT CCAAACTCTA
    GAGGAGCTGG AACAACTGTA CAGCAGAGAG CTGATTGTGG CCACGACTTG TATGGGTGTT
    AAACACCCAA TTTTCAGCCG CAGGCGCTTT GACTTCTGCA TCGTTGATGA GGCGTCTCAG
    ATCAGTCAGC CTGTGTGTAT AGGTCCACTG TTCTACGCCC AGCGCTTTGT CTTGGTGGGA
    GACCATCAAC AGCTTCCTCC TATTGTGCAG AATGCTGAGG CCAGGTCCTT GGGCATGGAT
    GAGAGTTTGT TCAAACGTCT GGAACACCAC AGGGATGCTG TTGTTCAGCT GAATGTGCAG
    TACAGGATGA ACAGTGCGAT CATGTCTCTC AGCAATGCTC TGATGTATGA GGGCCGGCTG
    GAGTGTGGCT CTGAGAAGAC GGCAAACGCA GTGCTTCAGC TGCCCAGTAG AGCTCAGGCA
    GAGGAGGAGC TGGAGCTGTA CGTGTGTCAG CCGCAGTACA GTGCTTGGGT CCAGGCCGCA
    CTGGAGCCCA ACAGTCCTGT CTGTTTCCTG GACACCTCTG AGGTTGCAGC CCCTGAAACT
    GTGGAGAAAA GTGGGATCAG TAATCACACT GAAGCTGTTT TAGTACAAGG CCTTGTTACT
    CTGCTGCTTA AGGCTGGATG CAGAGCATGT GATATTGGGG TCATCGCTCC GTATCGACAA
    CAGCTGAAAG TTATCTCCAG TCTGCTTCAG GGAGACGCCT TTAAAGCACT TGAAGTCAAC
    ACTGTGGACA AATACCAAGG ACGTGACAAG AGCGTCATCA TCGTGTCTTT TGTTAGAAGT
    AATGCTGAAG GCAACCTGGG AGAGTTGCTC CAGGACTGGC GTAGGCTGAA TGTGGCCATC
    ACTAGAGCCA AACACAAGCT CCTCATGCTG GGCTCTGCCC CCACTCTTCG CCGGTACGCA
    CCGCTGGAGA AACTTCTCTG CCACCTTCAG CAGGAAGATA TGATCTTCCA GCTACCTCCT
    GCTGCACACG AGGGACTACC TGGAGTCATC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18850
    Clone ID Related Accession (Same CDS sequence) XM_021480834.1 , XM_021474283.1 , XM_682882.7
    Accession Version XM_021480834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336509.1
    CDS287..4411
    Translation

    Target ORF information:

    RefSeq Version XM_021480834.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18850
    Clone ID Related Accession (Same CDS sequence) XM_021480834.1 , XM_021474283.1 , XM_682882.7
    Accession Version XM_021474283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395309.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329958.1
    CDS317..4441
    Translation

    Target ORF information:

    RefSeq Version XM_021474283.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18850
    Clone ID Related Accession (Same CDS sequence) XM_021480834.1 , XM_021474283.1 , XM_682882.7
    Accession Version XM_682882.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product DNA replication ATP-dependent helicase/nuclease DNA2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682882.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687974.5
    CDS304..4428
    Translation

    Target ORF information:

    RefSeq Version XM_682882.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_682882.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
    AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA
    GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC
    TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT
    TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC
    AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA
    GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG
    TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG
    AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA
    TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG
    GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC
    TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT
    TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC
    TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT
    ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG
    AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT
    TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG
    AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT
    CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG
    AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA
    GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT
    GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG
    CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA
    GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT
    GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT
    GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT
    CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA
    CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT
    GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC
    ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT
    GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG
    AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG
    ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC
    AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC
    TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC
    CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC
    TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA
    CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC
    CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT
    AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG
    CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG
    AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT
    CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA
    GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG
    GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG
    GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG
    TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT
    CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT
    TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG
    CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG
    GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT
    CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC
    CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT
    ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG
    GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC
    TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG
    GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG
    AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG
    GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA
    GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC
    CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC
    CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC
    CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG
    TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT
    GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT
    GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT
    GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC
    CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG
    CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.